COL20A1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene
Clones
gRNAs

Summary

Gene Symbol	COL20A1
Entrez Gene ID	485971
Full Name	collagen type XX alpha 1 chain
General protein information	Preferred Names collagen type XX alpha 1 chain Names collagen alpha-1(XX) chain
Gene Type	protein-coding
Organism	Canis lupus familiaris(dog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_014106882.1	XP_013962357.1	collagen alpha-1(XX) chain
XM_022409235.1	XP_022264943.1	collagen alpha-1(XX) chain isoform X3
XM_022409236.1	XP_022264944.1	collagen alpha-1(XX) chain isoform X4
XM_022409234.1	XP_022264942.1	collagen alpha-1(XX) chain isoform X2
XM_022409233.1	XP_022264941.1	collagen alpha-1(XX) chain isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Col20a1	XP_006235874.1
Homo sapiens (human)	COL20A1	NP_065933.2
Pan troglodytes (chimpanzee)	COL20A1	XP_001148451.1
Bos taurus (cattle)	COL20A1	XP_002692297.2
Mus musculus (house mouse)	Col20a1	NP_082794.1
Gallus gallus (chicken)	COL20A1	NP_001004392.1
Canis lupus familiaris (dog)	COL20A1	XP_543096.3

Gene Ontology

The following COL20A1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the COL20A1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCe296842	XM_022409233.1 Latest version!	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe296843	XM_022409234.1 Latest version!	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe296844	XM_022409235.1 Latest version!	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
OCe296845	XM_022409236.1 Latest version!	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
OCe42670	XM_014106882.1 Latest version!	Canis lupus familiaris collagen, type XX, alpha 1 (COL20A1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

COL20A1 ( XM_022409233.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264941.1 Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCe296842
Clone ID Related Accession (Same CDS sequence)	XM_022409233.1
Accession Version	XM_022409233.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4323bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	collagen alpha-1(XX) chain isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGTAGGTG CTGCCCCTCC CCCACCCCCT 
TCCTCCCCAA CGGACCAGCC GCTGAGGCTC TCTCCTCTCC AGGGCCCGCC GGGGGTCAAA 
GGAGAGAAGG GGGACCATGG GCTCCCGGGC CTGCAGGGCC ACCCCGGTCA CCAGGGCGCC 
CCCGGGAAGG TCGGCCTCCA GGGACCAAAG GGAATGAGAG GCCTGGAGGG GAGCGCTGGC 
CTGCCTGGAC CCCCCGGCCC GAGGGGCTTC CAGGGCATGG CGGGAGCCAG GGGCACCGCA 
GGGGAGAGAG GCCCCCCGGG GGCCGTGGGG CCCACGGGGC TGCCGGGACC CAAAGGGGAG 
CGAGGAGAGA AGGTGAGGCG GCCCCTGTCT AGGGTCCGGG CACTTCCTGG GGAGCAGGGG 
GGCAGCTGGC CACGTGGCCC GTCCTCCCTT GCAGGGCGAG CCACAGTCCC TGGCCACCAT 
CTCCCAGCTG CTGAGCCGGG TCTGCGAGTC TGCCATCCAG ACTCACGTGC TGAAGCTCCA 
CTCCTTCCTC CACGAGAGCG CCAGGCCCCC CCGGCCCATC TTGGAGGCGC CCAAGCTCCC 
TGGACGAGGA GGACAGGACA GTCCCCGCCC CGAGGACAGA GGCGAGCCCA GAACCCTCGG 
TCCCATGGGC AGCCCTCGGC CGAAAGGGAG CGAGCCTCCA AGACCCCTGA CAGGAACGGG 
ACGGCCCCCG TGCTCCCCAC AGTGAGCCTG CAACCCCTCC CCAGCCTCCA GCACCAAGAC 
GTCCCGGGAC GACCTGGAGC AAAAGGAGGG CGGTGGGGAG CCCTTCCTCT GTACGCTTTC 
CGCACTCAGA TAAACCGCCG CATGCTTGTC ACCACGGGGC TGCTCACTGC AGGGAGGGGT 
GTCCCAGGGC CCAGAGTTCG GCCCTGCCAA GTGGAGTCCA AGGCTCCCCC CAGTCTCCCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264941.1
CDS	143..4465
Translation	MTAPAGPRLGLRLCLWLGAALGLGQGPASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPMAGDAEQEVMLTTKTPKATVGGLSPSKGYTVQIFELTGSGNALLAQREFVIEDLKSHSGSRSDQKPLGAAPEPTPPDVGCPDPEPLGTSSPSQDPPTLAGSDWGKAGPQFRCTPPSPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPSKVQVGLTQYSGDPQTEWDLNALRTKEDVLAAVRRLRYKGGNTFTGLALTHVLEHNLRPAAGPRPEATKVLILVTDGKSQDDARAAGRILKDLGVAIFAVGVKNADEAELQLLASQPLDITVHNVQDFPQLGTLAGLLGRLICQKVQGGSRDPARPAAATPALDTLFTPSSLLLTQVTSSSAHLSWTPAAQLPLKYLIVWRPSRGGPPREVVVDGPTLSAELHDLAAGTEYSVSVIPVGKAGVGEGLRGLVTTAPLPPPQALTVAAVTPRTIRLTWRPSAGATQYLVRCLPASPAGQEEGREVRVGQPEVLLDGLEPGRDYDVRVQSLQGTEASEARGVRARTSTLAPPRHLGFSDVSHDSARVFWDGLPRPVRLFRVSFFSSDGSHSGQVEVPGNATSASLGPLSSSTTYAVRVTCLYPGGGSSTLTGHLTTRKVPSPSQLVVTRLPGDEARLQWAAASGVLVYQIKWTPLGDGKAHEVSVPGSRAMAVLPGLRSHLEYDITILAYYRDGARSDPVSLRYSPPRRSPPSDLTLASESPNSLRVSWTPPSGPVLHYRLTYALASGSGPEKSISVPGPRSHVTLPDLLAATKYRVLVSAVYGAGESPTVSATGRTAACPALSPDSPLPGFDLMRAFGLVEKEYASVRGVAMEPSAFGRARTFTILRDAQLSRRASAPHLAAPPPEHTVVFLLRVLPETPRETFALWQMTADDFQPVLGVLLDAGRRSLTYFHHDRTAALQEVTFDLPEVKRIFFGSFHKVHVAVGRSKVGLYVDCRKVAERPIGEAGSPPAAGFMMLGRLAKARGPRSSSASFQLQMLQIVCNDSWADEDVCCELPASKDGETCPAFASACTCASQTPALPGPQGPPGLPGRNGAPGERGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTVQGPVVGAAPPPPPSSPTDQPLRLSPLQGPPGVKGEKGDHGLPGLQGHPGHQGAPGKVGLQGPKGMRGLEGSAGLPGPPGPRGFQGMAGARGTAGERGPPGAVGPTGLPGPKGERGEKVRRPLSRVRALPGEQGGSWPRGPSSLAGRATVPGHHLPAAEPGLRVCHPDSRAEAPLLPPRERQAPPAHLGGAQAPWTRRTGQSPPRGQRRAQNPRSHGQPSAERERASKTPDRNGTAPVLPTVSLQPLPSLQHQDVPGRPGAKGGRWGALPLYAFRTQINRRMLVTTGLLTAGRGVPGPRVRPCQVESKAPPSLP

Target ORF information:

RefSeq Version	XM_022409233.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409233.1

ORF Insert Sequence:

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGTAGGTG CTGCCCCTCC CCCACCCCCT 
TCCTCCCCAA CGGACCAGCC GCTGAGGCTC TCTCCTCTCC AGGGCCCGCC GGGGGTCAAA 
GGAGAGAAGG GGGACCATGG GCTCCCGGGC CTGCAGGGCC ACCCCGGTCA CCAGGGCGCC 
CCCGGGAAGG TCGGCCTCCA GGGACCAAAG GGAATGAGAG GCCTGGAGGG GAGCGCTGGC 
CTGCCTGGAC CCCCCGGCCC GAGGGGCTTC CAGGGCATGG CGGGAGCCAG GGGCACCGCA 
GGGGAGAGAG GCCCCCCGGG GGCCGTGGGG CCCACGGGGC TGCCGGGACC CAAAGGGGAG 
CGAGGAGAGA AGGTGAGGCG GCCCCTGTCT AGGGTCCGGG CACTTCCTGG GGAGCAGGGG 
GGCAGCTGGC CACGTGGCCC GTCCTCCCTT GCAGGGCGAG CCACAGTCCC TGGCCACCAT 
CTCCCAGCTG CTGAGCCGGG TCTGCGAGTC TGCCATCCAG ACTCACGTGC TGAAGCTCCA 
CTCCTTCCTC CACGAGAGCG CCAGGCCCCC CCGGCCCATC TTGGAGGCGC CCAAGCTCCC 
TGGACGAGGA GGACAGGACA GTCCCCGCCC CGAGGACAGA GGCGAGCCCA GAACCCTCGG 
TCCCATGGGC AGCCCTCGGC CGAAAGGGAG CGAGCCTCCA AGACCCCTGA CAGGAACGGG 
ACGGCCCCCG TGCTCCCCAC AGTGAGCCTG CAACCCCTCC CCAGCCTCCA GCACCAAGAC 
GTCCCGGGAC GACCTGGAGC AAAAGGAGGG CGGTGGGGAG CCCTTCCTCT GTACGCTTTC 
CGCACTCAGA TAAACCGCCG CATGCTTGTC ACCACGGGGC TGCTCACTGC AGGGAGGGGT 
GTCCCAGGGC CCAGAGTTCG GCCCTGCCAA GTGGAGTCCA AGGCTCCCCC CAGTCTCCCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_022409234.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264942.1 Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OCe296843
Clone ID Related Accession (Same CDS sequence)	XM_022409234.1
Accession Version	XM_022409234.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	collagen alpha-1(XX) chain isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGGCCCGC CGGGGGTCAA AGGAGAGAAG 
GGGGACCATG GGCTCCCGGG CCTGCAGGGC CACCCCGGTC ACCAGGGCGC CCCCGGGAAG 
GTCGGCCTCC AGGGACCAAA GGGAATGAGA GGCCTGGAGG GGAGCGCTGG CCTGCCTGGA 
CCCCCCGGCC CGAGGGGCTT CCAGGGCATG GCGGGAGCCA GGGGCACCGC AGGGGAGAGA 
GGCCCCCCGG GGGCCGTGGG GCCCACGGGG CTGCCGGGAC CCAAAGGGGA GCGAGGAGAG 
AAGGTGAGGC GGCCCCTGTC TAGGGTCCGG GCACTTCCTG GGGAGCAGGG GGGCAGCTGG 
CCACGTGGCC CGTCCTCCCT TGCAGGGCGA GCCACAGTCC CTGGCCACCA TCTCCCAGCT 
GCTGAGCCGG GTCTGCGAGT CTGCCATCCA GACTCACGTG CTGAAGCTCC ACTCCTTCCT 
CCACGAGAGC GCCAGGCCCC CCCGGCCCAT CTTGGAGGCG CCCAAGCTCC CTGGACGAGG 
AGGACAGGAC AGTCCCCGCC CCGAGGACAG AGGCGAGCCC AGAACCCTCG GTCCCATGGG 
CAGCCCTCGG CCGAAAGGGA GCGAGCCTCC AAGACCCCTG ACAGGAACGG GACGGCCCCC 
GTGCTCCCCA CAGTGAGCCT GCAACCCCTC CCCAGCCTCC AGCACCAAGA CGTCCCGGGA 
CGACCTGGAG CAAAAGGAGG GCGGTGGGGA GCCCTTCCTC TGTACGCTTT CCGCACTCAG 
ATAAACCGCC GCATGCTTGT CACCACGGGG CTGCTCACTG CAGGGAGGGG TGTCCCAGGG 
CCCAGAGTTC GGCCCTGCCA AGTGGAGTCC AAGGCTCCCC CCAGTCTCCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264942.1
CDS	143..4396
Translation	MTAPAGPRLGLRLCLWLGAALGLGQGPASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPMAGDAEQEVMLTTKTPKATVGGLSPSKGYTVQIFELTGSGNALLAQREFVIEDLKSHSGSRSDQKPLGAAPEPTPPDVGCPDPEPLGTSSPSQDPPTLAGSDWGKAGPQFRCTPPSPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPSKVQVGLTQYSGDPQTEWDLNALRTKEDVLAAVRRLRYKGGNTFTGLALTHVLEHNLRPAAGPRPEATKVLILVTDGKSQDDARAAGRILKDLGVAIFAVGVKNADEAELQLLASQPLDITVHNVQDFPQLGTLAGLLGRLICQKVQGGSRDPARPAAATPALDTLFTPSSLLLTQVTSSSAHLSWTPAAQLPLKYLIVWRPSRGGPPREVVVDGPTLSAELHDLAAGTEYSVSVIPVGKAGVGEGLRGLVTTAPLPPPQALTVAAVTPRTIRLTWRPSAGATQYLVRCLPASPAGQEEGREVRVGQPEVLLDGLEPGRDYDVRVQSLQGTEASEARGVRARTSTLAPPRHLGFSDVSHDSARVFWDGLPRPVRLFRVSFFSSDGSHSGQVEVPGNATSASLGPLSSSTTYAVRVTCLYPGGGSSTLTGHLTTRKVPSPSQLVVTRLPGDEARLQWAAASGVLVYQIKWTPLGDGKAHEVSVPGSRAMAVLPGLRSHLEYDITILAYYRDGARSDPVSLRYSPPRRSPPSDLTLASESPNSLRVSWTPPSGPVLHYRLTYALASGSGPEKSISVPGPRSHVTLPDLLAATKYRVLVSAVYGAGESPTVSATGRTAACPALSPDSPLPGFDLMRAFGLVEKEYASVRGVAMEPSAFGRARTFTILRDAQLSRRASAPHLAAPPPEHTVVFLLRVLPETPRETFALWQMTADDFQPVLGVLLDAGRRSLTYFHHDRTAALQEVTFDLPEVKRIFFGSFHKVHVAVGRSKVGLYVDCRKVAERPIGEAGSPPAAGFMMLGRLAKARGPRSSSASFQLQMLQIVCNDSWADEDVCCELPASKDGETCPAFASACTCASQTPALPGPQGPPGLPGRNGAPGERGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGAPGKVGLQGPKGMRGLEGSAGLPGPPGPRGFQGMAGARGTAGERGPPGAVGPTGLPGPKGERGEKVRRPLSRVRALPGEQGGSWPRGPSSLAGRATVPGHHLPAAEPGLRVCHPDSRAEAPLLPPRERQAPPAHLGGAQAPWTRRTGQSPPRGQRRAQNPRSHGQPSAERERASKTPDRNGTAPVLPTVSLQPLPSLQHQDVPGRPGAKGGRWGALPLYAFRTQINRRMLVTTGLLTAGRGVPGPRVRPCQVESKAPPSLP

Target ORF information:

RefSeq Version	XM_022409234.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409234.1

ORF Insert Sequence:

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGGCCCGC CGGGGGTCAA AGGAGAGAAG 
GGGGACCATG GGCTCCCGGG CCTGCAGGGC CACCCCGGTC ACCAGGGCGC CCCCGGGAAG 
GTCGGCCTCC AGGGACCAAA GGGAATGAGA GGCCTGGAGG GGAGCGCTGG CCTGCCTGGA 
CCCCCCGGCC CGAGGGGCTT CCAGGGCATG GCGGGAGCCA GGGGCACCGC AGGGGAGAGA 
GGCCCCCCGG GGGCCGTGGG GCCCACGGGG CTGCCGGGAC CCAAAGGGGA GCGAGGAGAG 
AAGGTGAGGC GGCCCCTGTC TAGGGTCCGG GCACTTCCTG GGGAGCAGGG GGGCAGCTGG 
CCACGTGGCC CGTCCTCCCT TGCAGGGCGA GCCACAGTCC CTGGCCACCA TCTCCCAGCT 
GCTGAGCCGG GTCTGCGAGT CTGCCATCCA GACTCACGTG CTGAAGCTCC ACTCCTTCCT 
CCACGAGAGC GCCAGGCCCC CCCGGCCCAT CTTGGAGGCG CCCAAGCTCC CTGGACGAGG 
AGGACAGGAC AGTCCCCGCC CCGAGGACAG AGGCGAGCCC AGAACCCTCG GTCCCATGGG 
CAGCCCTCGG CCGAAAGGGA GCGAGCCTCC AAGACCCCTG ACAGGAACGG GACGGCCCCC 
GTGCTCCCCA CAGTGAGCCT GCAACCCCTC CCCAGCCTCC AGCACCAAGA CGTCCCGGGA 
CGACCTGGAG CAAAAGGAGG GCGGTGGGGA GCCCTTCCTC TGTACGCTTT CCGCACTCAG 
ATAAACCGCC GCATGCTTGT CACCACGGGG CTGCTCACTG CAGGGAGGGG TGTCCCAGGG 
CCCAGAGTTC GGCCCTGCCA AGTGGAGTCC AAGGCTCCCC CCAGTCTCCC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_022409235.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264943.1 Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.

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CloneID	OCe296844
Clone ID Related Accession (Same CDS sequence)	XM_022409235.1
Accession Version	XM_022409235.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4023bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	collagen alpha-1(XX) chain isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGTAGGTG CTGCCCCTCC CCCACCCCCT 
TCCTCCCCAA CGGACCAGCC GCTGAGGCTC TCTCCTCTCC AGGGCCCGCC GGGGGTCAAA 
GGAGAGAAGG GGGACCATGG GCTCCCGGGC CTGCAGGGCC ACCCCGGTCA CCAGGGCGCC 
CCCGGGAAGG TCGGCCTCCA GGGACCAAAG GGAATGAGAG GCCTGGAGGG GAGCGCTGGC 
CTGCCTGGAC CCCCCGGCCC GAGGGGCTTC CAGGGCATGG CGGGAGCCAG GGGCACCGCA 
GGGGAGAGAG GCCCCCCGGG GGCCGTGGGG CCCACGGGGC TGCCGGGACC CAAAGGGGAG 
CGAGGAGAGA AGGGCGAGCC ACAGTCCCTG GCCACCATCT CCCAGCTGCT GAGCCGGGTC 
TGCGAGTCTG CCATCCAGAC TCACGTGCTG AAGCTCCACT CCTTCCTCCA CGAGAGCGCC 
AGGCCCCCCC GGCCCATCTT GGAGGCGCCC AAGCTCCCTG GACGAGGAGG ACAGGACAGT 
CCCCGCCCCG AGGACAGAGG CGAGCCCAGA ACCCTCGGTC CCATGGGCAG CCCTCGGCCG 
AAAGGGAGCG AGCCTCCAAG ACCCCTGACA GGAACGGGAC GGCCCCCGTG CTCCCCACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264943.1
CDS	143..4165
Translation	MTAPAGPRLGLRLCLWLGAALGLGQGPASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPMAGDAEQEVMLTTKTPKATVGGLSPSKGYTVQIFELTGSGNALLAQREFVIEDLKSHSGSRSDQKPLGAAPEPTPPDVGCPDPEPLGTSSPSQDPPTLAGSDWGKAGPQFRCTPPSPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPSKVQVGLTQYSGDPQTEWDLNALRTKEDVLAAVRRLRYKGGNTFTGLALTHVLEHNLRPAAGPRPEATKVLILVTDGKSQDDARAAGRILKDLGVAIFAVGVKNADEAELQLLASQPLDITVHNVQDFPQLGTLAGLLGRLICQKVQGGSRDPARPAAATPALDTLFTPSSLLLTQVTSSSAHLSWTPAAQLPLKYLIVWRPSRGGPPREVVVDGPTLSAELHDLAAGTEYSVSVIPVGKAGVGEGLRGLVTTAPLPPPQALTVAAVTPRTIRLTWRPSAGATQYLVRCLPASPAGQEEGREVRVGQPEVLLDGLEPGRDYDVRVQSLQGTEASEARGVRARTSTLAPPRHLGFSDVSHDSARVFWDGLPRPVRLFRVSFFSSDGSHSGQVEVPGNATSASLGPLSSSTTYAVRVTCLYPGGGSSTLTGHLTTRKVPSPSQLVVTRLPGDEARLQWAAASGVLVYQIKWTPLGDGKAHEVSVPGSRAMAVLPGLRSHLEYDITILAYYRDGARSDPVSLRYSPPRRSPPSDLTLASESPNSLRVSWTPPSGPVLHYRLTYALASGSGPEKSISVPGPRSHVTLPDLLAATKYRVLVSAVYGAGESPTVSATGRTAACPALSPDSPLPGFDLMRAFGLVEKEYASVRGVAMEPSAFGRARTFTILRDAQLSRRASAPHLAAPPPEHTVVFLLRVLPETPRETFALWQMTADDFQPVLGVLLDAGRRSLTYFHHDRTAALQEVTFDLPEVKRIFFGSFHKVHVAVGRSKVGLYVDCRKVAERPIGEAGSPPAAGFMMLGRLAKARGPRSSSASFQLQMLQIVCNDSWADEDVCCELPASKDGETCPAFASACTCASQTPALPGPQGPPGLPGRNGAPGERGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTVQGPVVGAAPPPPPSSPTDQPLRLSPLQGPPGVKGEKGDHGLPGLQGHPGHQGAPGKVGLQGPKGMRGLEGSAGLPGPPGPRGFQGMAGARGTAGERGPPGAVGPTGLPGPKGERGEKGEPQSLATISQLLSRVCESAIQTHVLKLHSFLHESARPPRPILEAPKLPGRGGQDSPRPEDRGEPRTLGPMGSPRPKGSEPPRPLTGTGRPPCSPQ

Target ORF information:

RefSeq Version	XM_022409235.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409235.1

ORF Insert Sequence:

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGTAGGTG CTGCCCCTCC CCCACCCCCT 
TCCTCCCCAA CGGACCAGCC GCTGAGGCTC TCTCCTCTCC AGGGCCCGCC GGGGGTCAAA 
GGAGAGAAGG GGGACCATGG GCTCCCGGGC CTGCAGGGCC ACCCCGGTCA CCAGGGCGCC 
CCCGGGAAGG TCGGCCTCCA GGGACCAAAG GGAATGAGAG GCCTGGAGGG GAGCGCTGGC 
CTGCCTGGAC CCCCCGGCCC GAGGGGCTTC CAGGGCATGG CGGGAGCCAG GGGCACCGCA 
GGGGAGAGAG GCCCCCCGGG GGCCGTGGGG CCCACGGGGC TGCCGGGACC CAAAGGGGAG 
CGAGGAGAGA AGGGCGAGCC ACAGTCCCTG GCCACCATCT CCCAGCTGCT GAGCCGGGTC 
TGCGAGTCTG CCATCCAGAC TCACGTGCTG AAGCTCCACT CCTTCCTCCA CGAGAGCGCC 
AGGCCCCCCC GGCCCATCTT GGAGGCGCCC AAGCTCCCTG GACGAGGAGG ACAGGACAGT 
CCCCGCCCCG AGGACAGAGG CGAGCCCAGA ACCCTCGGTC CCATGGGCAG CCCTCGGCCG 
AAAGGGAGCG AGCCTCCAAG ACCCCTGACA GGAACGGGAC GGCCCCCGTG CTCCCCACAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_022409236.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_022264944.1 Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.

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CloneID	OCe296845
Clone ID Related Accession (Same CDS sequence)	XM_022409236.1
Accession Version	XM_022409236.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3954bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-09-04
Organism	Canis lupus familiaris(dog)
Product	collagen alpha-1(XX) chain isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006606.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGGCCCGC CGGGGGTCAA AGGAGAGAAG 
GGGGACCATG GGCTCCCGGG CCTGCAGGGC CACCCCGGTC ACCAGGGCGC CCCCGGGAAG 
GTCGGCCTCC AGGGACCAAA GGGAATGAGA GGCCTGGAGG GGAGCGCTGG CCTGCCTGGA 
CCCCCCGGCC CGAGGGGCTT CCAGGGCATG GCGGGAGCCA GGGGCACCGC AGGGGAGAGA 
GGCCCCCCGG GGGCCGTGGG GCCCACGGGG CTGCCGGGAC CCAAAGGGGA GCGAGGAGAG 
AAGGGCGAGC CACAGTCCCT GGCCACCATC TCCCAGCTGC TGAGCCGGGT CTGCGAGTCT 
GCCATCCAGA CTCACGTGCT GAAGCTCCAC TCCTTCCTCC ACGAGAGCGC CAGGCCCCCC 
CGGCCCATCT TGGAGGCGCC CAAGCTCCCT GGACGAGGAG GACAGGACAG TCCCCGCCCC 
GAGGACAGAG GCGAGCCCAG AACCCTCGGT CCCATGGGCA GCCCTCGGCC GAAAGGGAGC 
GAGCCTCCAA GACCCCTGAC AGGAACGGGA CGGCCCCCGT GCTCCCCACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_022264944.1
CDS	143..4096
Translation	MTAPAGPRLGLRLCLWLGAALGLGQGPASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPMAGDAEQEVMLTTKTPKATVGGLSPSKGYTVQIFELTGSGNALLAQREFVIEDLKSHSGSRSDQKPLGAAPEPTPPDVGCPDPEPLGTSSPSQDPPTLAGSDWGKAGPQFRCTPPSPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPSKVQVGLTQYSGDPQTEWDLNALRTKEDVLAAVRRLRYKGGNTFTGLALTHVLEHNLRPAAGPRPEATKVLILVTDGKSQDDARAAGRILKDLGVAIFAVGVKNADEAELQLLASQPLDITVHNVQDFPQLGTLAGLLGRLICQKVQGGSRDPARPAAATPALDTLFTPSSLLLTQVTSSSAHLSWTPAAQLPLKYLIVWRPSRGGPPREVVVDGPTLSAELHDLAAGTEYSVSVIPVGKAGVGEGLRGLVTTAPLPPPQALTVAAVTPRTIRLTWRPSAGATQYLVRCLPASPAGQEEGREVRVGQPEVLLDGLEPGRDYDVRVQSLQGTEASEARGVRARTSTLAPPRHLGFSDVSHDSARVFWDGLPRPVRLFRVSFFSSDGSHSGQVEVPGNATSASLGPLSSSTTYAVRVTCLYPGGGSSTLTGHLTTRKVPSPSQLVVTRLPGDEARLQWAAASGVLVYQIKWTPLGDGKAHEVSVPGSRAMAVLPGLRSHLEYDITILAYYRDGARSDPVSLRYSPPRRSPPSDLTLASESPNSLRVSWTPPSGPVLHYRLTYALASGSGPEKSISVPGPRSHVTLPDLLAATKYRVLVSAVYGAGESPTVSATGRTAACPALSPDSPLPGFDLMRAFGLVEKEYASVRGVAMEPSAFGRARTFTILRDAQLSRRASAPHLAAPPPEHTVVFLLRVLPETPRETFALWQMTADDFQPVLGVLLDAGRRSLTYFHHDRTAALQEVTFDLPEVKRIFFGSFHKVHVAVGRSKVGLYVDCRKVAERPIGEAGSPPAAGFMMLGRLAKARGPRSSSASFQLQMLQIVCNDSWADEDVCCELPASKDGETCPAFASACTCASQTPALPGPQGPPGLPGRNGAPGERGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGAPGKVGLQGPKGMRGLEGSAGLPGPPGPRGFQGMAGARGTAGERGPPGAVGPTGLPGPKGERGEKGEPQSLATISQLLSRVCESAIQTHVLKLHSFLHESARPPRPILEAPKLPGRGGQDSPRPEDRGEPRTLGPMGSPRPKGSEPPRPLTGTGRPPCSPQ

Target ORF information:

RefSeq Version	XM_022409236.1
Organism	Canis lupus familiaris(dog)
Definition	Canis lupus familiaris collagen type XX alpha 1 chain (COL20A1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_022409236.1

ORF Insert Sequence:

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGCCGGACCC CAGTTCCGCT GCACACCCCC CAGCCCCGTG 
GACATGATCT TCCTGGTGGA CGGGTCCTGG AGCATCGGCC ACAGTCACTT CCAGCAGGTC 
AAAGATTTCC TGGCCAGCGT CATCGAGCCC TTTGAAATCG GGCCAAGTAA AGTCCAAGTC 
GGCTTGACTC AGTACAGCGG AGACCCCCAG ACCGAGTGGG ACCTCAACGC CCTCCGCACC 
AAGGAGGACG TGCTGGCAGC TGTCCGCCGT CTGCGCTACA AAGGGGGAAA CACGTTCACC 
GGCCTGGCGC TGACGCACGT GCTCGAGCAC AACCTGCGGC CCGCAGCGGG GCCCCGGCCT 
GAGGCCACCA AGGTGCTGAT CCTGGTGACA GACGGCAAAT CCCAGGACGA TGCCCGCGCC 
GCTGGCCGCA TCCTCAAGGA CCTGGGTGTT GCCATCTTTG CTGTGGGTGT GAAGAACGCG 
GACGAGGCTG AGCTGCAGCT CCTGGCGTCC CAGCCACTGG ACATCACCGT CCACAACGTG 
CAGGACTTCC CGCAGCTCGG CACCTTGGCC GGCTTGCTCG GCCGCCTCAT CTGCCAGAAG 
GTCCAGGGCG GGAGCAGGGA CCCGGCCCGG CCGGCAGCAG CCACCCCCGC CCTGGACACC 
CTCTTCACTC CCAGCAGCCT GCTGTTGACC CAGGTGACCT CCTCCAGCGC CCACCTGTCC 
TGGACTCCAG CCGCCCAGCT GCCCCTCAAG TATCTGATCG TGTGGCGGCC CTCGAGGGGT 
GGCCCGCCCC GGGAGGTGGT GGTGGACGGG CCCACCTTGT CCGCGGAGCT GCACGACCTG 
GCCGCCGGCA CGGAGTACTC GGTGTCTGTG ATCCCCGTCG GCAAGGCCGG GGTCGGCGAG 
GGCCTGCGGG GCCTGGTGAC CACAGCGCCT CTGCCCCCGC CCCAGGCGCT GACCGTGGCT 
GCGGTGACAC CCAGAACCAT CCGCCTCACC TGGCGGCCCT CGGCCGGGGC CACACAGTAC 
CTGGTGCGAT GCCTGCCAGC CTCCCCTGCG GGCCAGGAGG AGGGCAGAGA GGTGCGGGTG 
GGGCAGCCCG AGGTGCTGCT GGACGGCCTG GAGCCTGGCA GGGACTACGA TGTCCGGGTG 
CAGAGCTTGC AGGGCACAGA GGCCAGCGAG GCCCGGGGCG TCCGCGCCAG GACCTCCACC 
CTGGCGCCCC CCAGACACCT GGGCTTCTCG GATGTGAGTC ACGACTCCGC ACGCGTGTTC 
TGGGACGGCC TCCCGAGGCC CGTCCGCCTG TTCAGGGTCA GCTTTTTCTC CAGCGACGGC 
AGCCACTCGG GGCAGGTGGA GGTTCCCGGG AATGCCACCT CGGCCTCTCT GGGCCCTCTG 
TCCTCCTCCA CCACGTATGC TGTCCGTGTC ACCTGCCTCT ACCCTGGGGG CGGCTCCTCC 
ACGTTGACCG GCCACCTGAC CACACGGAAG GTCCCCAGCC CCAGCCAGCT CGTGGTGACC 
AGGCTCCCCG GGGACGAGGC TCGGCTGCAG TGGGCGGCAG CTTCCGGAGT GCTCGTCTAT 
CAGATCAAGT GGACACCCCT GGGAGACGGG AAGGCCCACG AGGTCTCTGT CCCCGGGAGC 
CGGGCCATGG CAGTCCTACC TGGCCTGCGA AGCCACCTGG AGTACGACAT CACCATCCTG 
GCCTACTACA GGGACGGGGC CCGCAGCGAC CCGGTGTCCC TGCGCTACAG CCCCCCGCGC 
CGCAGCCCGC CGTCTGACCT GACCCTGGCT TCCGAGTCGC CCAACAGCCT GCGAGTCAGC 
TGGACGCCCC CGAGCGGCCC CGTCCTCCAC TACCGGCTCA CCTATGCGCT GGCCTCGGGC 
TCGGGACCCG AGAAGTCGAT CTCTGTCCCA GGACCCAGGA GCCACGTGAC ACTGCCCGAC 
CTGCTGGCAG CCACCAAATA CAGGGTCCTG GTCTCGGCCG TCTACGGAGC AGGGGAGAGT 
CCGACAGTGT CTGCCACAGG CCGGACCGCA GCCTGCCCTG CGCTCAGCCC TGACAGCCCC 
CTCCCAGGCT TCGACCTCAT GAGGGCCTTC GGGCTGGTGG AGAAGGAGTA CGCCTCCGTC 
CGCGGCGTCG CCATGGAGCC CTCGGCTTTC GGCCGCGCCA GGACCTTCAC CATCCTCAGG 
GACGCCCAGC TGAGCCGCCG GGCCAGTGCC CCCCACCTGG CTGCGCCCCC GCCCGAGCAC 
ACGGTGGTCT TCCTCCTGCG TGTGCTGCCC GAGACCCCCC GGGAGACCTT TGCGCTGTGG 
CAGATGACGG CCGACGACTT CCAGCCCGTC CTGGGCGTCC TGCTGGACGC GGGCAGGAGG 
TCTCTGACCT ACTTCCACCA CGACCGCACG GCCGCCTTGC AAGAGGTCAC CTTTGACCTG 
CCCGAAGTGA AGAGGATATT CTTCGGAAGC TTCCACAAGG TGCACGTGGC CGTGGGCCGC 
TCCAAGGTCG GGCTGTACGT GGACTGCAGG AAGGTGGCCG AGAGGCCCAT CGGGGAGGCG 
GGCAGCCCGC CTGCCGCCGG CTTCATGATG CTGGGGAGGC TGGCCAAGGC GCGCGGGCCC 
CGGAGCAGCT CCGCCTCATT CCAGCTCCAG ATGCTGCAGA TCGTGTGCAA TGACTCCTGG 
GCAGACGAAG ACGTGTGCTG CGAGCTCCCT GCGTCGAAGG ACGGAGAGAC CTGCCCGGCC 
TTCGCGTCTG CCTGCACCTG CGCCTCGCAG ACCCCCGCGC TCCCGGGGCC CCAAGGACCT 
CCGGGCCTCC CTGGAAGGAA CGGAGCCCCA GGAGAGCGGG GCTTCCCGGG GCCCAGGGGA 
GAGCCTGGGC CACCCGGACA GATGGGACCA GAGGGTCCTG GAGGTCAGCA GGGCTCGCCA 
GGGACCCAGG GCCGGACTGT GCAGGGACCT GTGGGCCCGC CGGGGGTCAA AGGAGAGAAG 
GGGGACCATG GGCTCCCGGG CCTGCAGGGC CACCCCGGTC ACCAGGGCGC CCCCGGGAAG 
GTCGGCCTCC AGGGACCAAA GGGAATGAGA GGCCTGGAGG GGAGCGCTGG CCTGCCTGGA 
CCCCCCGGCC CGAGGGGCTT CCAGGGCATG GCGGGAGCCA GGGGCACCGC AGGGGAGAGA 
GGCCCCCCGG GGGCCGTGGG GCCCACGGGG CTGCCGGGAC CCAAAGGGGA GCGAGGAGAG 
AAGGGCGAGC CACAGTCCCT GGCCACCATC TCCCAGCTGC TGAGCCGGGT CTGCGAGTCT 
GCCATCCAGA CTCACGTGCT GAAGCTCCAC TCCTTCCTCC ACGAGAGCGC CAGGCCCCCC 
CGGCCCATCT TGGAGGCGCC CAAGCTCCCT GGACGAGGAG GACAGGACAG TCCCCGCCCC 
GAGGACAGAG GCGAGCCCAG AACCCTCGGT CCCATGGGCA GCCCTCGGCC GAAAGGGAGC 
GAGCCTCCAA GACCCCTGAC AGGAACGGGA CGGCCCCCGT GCTCCCCACA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

COL20A1 ( XM_014106882.1 ) cDNA ORF clone, Canis lupus familiaris(dog) -> XP_013962357.1 Canis lupus familiaris collagen, type XX, alpha 1 (COL20A1), mRNA.

Price & Availability↑

CloneID	OCe42670
Clone ID Related Accession (Same CDS sequence)	XM_014106882.1
Accession Version	XM_014106882.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-09-16
Organism	Canis lupus familiaris(Dog)
Product	collagen alpha-1(XX) chain
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726101.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 2% of CDS bases ##RefSeq-Attributes-END##

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGGTAAGTCC GGCAGGCGGA GTGACCCCCG CGTCTCAAGG 
TTTCACGGGT TCACTGTCCT GAGTCGCTCA GCCTCGTGGG GGCAGGTCAC CCGCTGGGGG 
TGTGCTCGGC CCGTGCCCCT TGCCTCCCAG CCCGCGGGTG GCAGCTCACC CGCCGCCATG 
AGCCCCGCGG CCCCTCAGCC ACCACCAACC CCGGGGAAGG GGATCCAGGG GCTTTGGGAG 
GGAGGTCGCG CGGTGACCAG TGACGTGGGC CTGGCCCAGC TGCTCACCAC CCTCCCTGCC 
CGCCCAGCCG GACCCCAGTT CCGCTGCACA CCCCCCAGCC CCGTGGACAT GATCTTCCTG 
GTGGACGGGT CCTGGAGCAT CGGCCACAGT CACTTCCAGC AGGTCAAAGA TTTCCTGGCC 
AGCGTCATCG AGCCCTTTGA AATCGGGCCA AGTAAAGTCC AAGTCGGCTT GACTCAGTAC 
AGCGGAGACC CCCAGACCGA GTGGGACCTC AACGCCCTCC GCACCAAGGA GGACGTGCTG 
GCAGCTGTCC GCCGTCTGCG CTACAAAGGG GGAAACACGT TCACCGGCCT GGCGCTGACG 
CACGTGCTCG AGCACAACCT GCGGCCCGCA GCGGGGCCCC GGCCTGAGGC CACCAAGGTG 
CTGATCCTGG TGACAGACGG CAAATCCCAG GACGATGCCC GCGCCGCTGG CCGCATCCTC 
AAGGACCTGG GTGTTGCCAT CTTTGCTGTG GGTGTGAAGA ACGCGGACGA GGCTGAGCTG 
CAGCTCCTGG CGTCCCAGCC ACTGGACATC ACCGTCCACA ACGTGCAGGA CTTCCCGCAG 
CTCGGCACCT TGGCCGGCTT GCTCGGCCGC CTCATCTGCC AGAAGGTCCA GGGCGGGAGC 
AGGGACCCGG CCCGGCCGGC AGCAGCCACC CCCGCCCTGG ACACCCTCTT CACTCCCAGC 
AGCCTGCTGT TGACCCAGGT GACCTCCTCC AGCGCCCACC TGTCCTGGAC TCCAGCCGCC 
CAGCTGCCCC TCAAGTATCT GATCGTGTGG CGGCCCTCGA GGGGTGGCCC GCCCCGGGAG 
GTGGTGGTGG ACGGGCCCAC CTTGTCCGCG GAGCTGCACG ACCTGGCCGC CGGCACGGAG 
TACTCGGTGT CTGTGATCCC CGTCGGCAAG GCCGGGGTCG GCGAGGGCCT GCGGGGCCTG 
GTGACCACAG CGCCTCTGCC CCCGCCCCAG GCGCTGACCG TGGCTGCGGT GACACCCAGA 
ACCATCCGCC TCACCTGGCG GCCCTCGGCC GGGGCCACAC AGTACCTGGT GCGATGCCTG 
CCAGCCTCCC CTGCGGGCCA GGAGGAGGGC AGAGAGGTGC GGGTGGGGCA GCCCGAGGTG 
CTGCTGGACG GCCTGGAGCC TGGCAGGGAC TACGATGTCC GGGTGCAGAG CTTGCAGGGC 
ACAGAGGCCA GCGAGGCCCG GGGCGTCCGC GCCAGGACCT CCACCCTGGC GCCCCCCAGA 
CACCTGGGCT TCTCGGATGT GAGTCACGAC TCCGCACGCG TGTTCTGGGA CGGCCTCCCG 
AGGCCCGTCC GCCTGTTCAG GGTCAGCTTT TTCTCCAGCG ACGGCAGCCA CTCGGGGCAG 
GTGGAGGTTC CCGGGAATGC CACCTCGGCC TCTCTGGGCC CTCTGTCCTC CTCCACCACG 
TATGCTGTCC GTGTCACCTG CCTCTACCCT GGGGGCGGCT CCTCCACGTT GACCGGCCAC 
CTGACCACAC GGAAGGTCCC CAGCCCCAGC CAGCTCGTGG TGACCAGGCT CCCCGGGGAC 
GAGGCTCGGC TGCAGTGGGC GGCAGCTTCC GGAGTGCTCG TCTATCAGAT CAAGTGGACA 
CCCCTGGGAG ACGGGAAGGC CCACGAGGTC TCTGTCCCCG GGAGCCGGGC CATGGCAGTC 
CTACCTGGCC TGCGAAGCCA CCTGGAGTAC GACATCACCA TCCTGGCCTA CTACAGGGAC 
GGGGCCCGCA GCGACCCGGT GTCCCTGCGC TACAGCCCCC CGCGCCGCAG CCCGCCGTCT 
GACCTGACCC TGGCTTCCGA GTCGCCCAAC AGCCTGCGAG TCAGCTGGAC GCCCCCGAGC 
GGCCCCGTCC TCCACTACCG GCTCACCTAT GCGCTGGCCT CGGGCTCGGG ACCCGAGAAG 
TCGATCTCTG TCCCAGGACC CAGGAGCCAC GTGACACTGC CCGACCTGCT GGCAGCCACC 
AAATACAGGG TCCTGGTCTC GGCCGTCTAC GGAGCAGGGG AGAGTCCGAC AGTGTCTGCC 
ACAGGCCGGA CCGCAGCCTG CCCTGCGCTC AGCCCTGACA GCCCCCTCCC AGGCTTCGAC 
CTCATGAGGG CCTTCGGGCT GGTGGAGAAG GAGTACGCCT CCGTCCGCGG CGTCGCCATG 
GAGCCCTCGG CTTTCGGCCG CGCCAGGACC TTCACCATCC TCAGGGACGC CCAGCTGAGC 
CGCCGGGCCA GTGCCCCCCA CCTGGCTGCG CCCCCGCCCG AGCACACGGT GGTCTTCCTC 
CTGCGTGTGC TGCCCGAGAC CCCCCGGGAG ACCTTTGCGC TGTGGCAGAT GACGGCCGAC 
GACTTCCAGC CCGTCCTGGG CGTCCTGCTG GACGCGGGCA GGAGGTCTCT GACCTACTTC 
CACCACGACC GCACGGCCGC CTTGCAAGAG GTCACCTTTG ACCTGCCCGA AGTGAAGAGG 
ATATTCTTCG GAAGCTTCCA CAAGGTGCAC GTGGCCGTGG GCCGCTCCAA GGTCGGGCTG 
TACGTGGACT GCAGGAAGGT GGCCGAGAGG CCCATCGGGG AGGCGGGCAG CCCGCCTGCC 
GCCGGCTTCA TGATGCTGGG GAGGCTGGCC AAGGCGCGCG GGCCCCGGAG CAGCTCCGCC 
TCATTCCAGC TCCAGATGCT GCAGATCGTG TGCAATGACT CCTGGGCAGA CGAAGACGTG 
TGCTGCGAGC TCCCTGCGTC GAAGGACGGA GAGACCTGCC CGGCCTTCGC GTCTGCCTGC 
ACCTGCGCCT CGCAGACCCC CGCGCTCCCG GGGCCCCAAG GACCTCCGGG CCTCCCTGGA 
AGGAACGGAG CCCCAGGAGA GCGGGGCTTC CCGGGGCCCA GGGGAGAGCC TGGGCCACCC 
GGACAGATGG GACCAGAGGG TCCTGGAGGT CAGCAGGGCT CGCCAGGGAC CCAGGGCCGG 
ACTGTGCAGG GACCTGTGGG CCCGCCGGGG GTCAAAGGAG AGAAGGGGGA CCATGGGCTC 
CCGGGCCTGC AGGGCCACCC CGGTCACCAG GGCGCCCCCG GGAAGGTCGG CCTCCAGGGA 
CCAAAGGGAA TGAGAGGCCT GGAGGGGAGC GCTGGCCTGC CTGGACCCCC CGGCCCGAGG 
GGCTTCCAGG GCATGGCGGG AGCCAGGGGC ACCGCAGGGG AGAGAGGCCC CCCGGGGGCC 
GTGGGGCCCA CGGGGCTGCC GGGACCCAAA GGGGAGCGAG GAGAGAAGGG CGAGCCACAG 
TCCCTGGCCA CCATCTCCCA GCTGCTGAGC CGGGTCTGCG AGTCTGCCAT CCAGACTCAC 
GTGCTGAAGC TCCACTCCTT CCTCCACGAG AGCGCCAGGC CCCCCCGGCC CATCTTGGAG 
GCGCCCAAGC TCCCTGGACG AGGAGGACAG GACAGTCCCC GCCCCGAGGA CAGAGGCGAG 
CCCAGAACCC TCGGTCCCAT GGGCAGCCCT CGGCCGAAAG GGAGCGAGCC TCCAAGACCC 
CTGACAGGAA CGGGTGAGCA CCCACCCAGG GGAGGGGAGA CTGGGGGGGC TGCAGGGTGG 
GGGTGCAGCA CGGATAAACA CGAAGCTGCC GAGAAGCAGG GCCTCTGGCT GGCTGGGCAC 
AGGGGGGTTC AGGGGCCTTG GGGGAGACTT GCAAGCCCGT GGGCAGATGT GGGGGAGCCA 
GGAGGGCCCA TGGTGGTTGT TAGCGCATCA GGAGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_013962357.1
CDS	1..4419
Translation	MTAPAGPRLGLRLCLWLGAALGLGQGPASGRLRLAVLPEDRLQMKWRESEGSSLGYLVQVKPMAGDAEQEVMLTTKTPKATVGGLSPSKGYTVQIFELTGSGNALLAQREFVIEDLKSHSGSRSDQKPLGAAPEPTPPDVGCPDPEPLGTSSPSQDPPTLAGSDWGKGKSGRRSDPRVSRFHGFTVLSRSASWGQVTRWGCARPVPLASQPAGGSSPAAMSPAAPQPPPTPGKGIQGLWEGGRAVTSDVGLAQLLTTLPARPAGPQFRCTPPSPVDMIFLVDGSWSIGHSHFQQVKDFLASVIEPFEIGPSKVQVGLTQYSGDPQTEWDLNALRTKEDVLAAVRRLRYKGGNTFTGLALTHVLEHNLRPAAGPRPEATKVLILVTDGKSQDDARAAGRILKDLGVAIFAVGVKNADEAELQLLASQPLDITVHNVQDFPQLGTLAGLLGRLICQKVQGGSRDPARPAAATPALDTLFTPSSLLLTQVTSSSAHLSWTPAAQLPLKYLIVWRPSRGGPPREVVVDGPTLSAELHDLAAGTEYSVSVIPVGKAGVGEGLRGLVTTAPLPPPQALTVAAVTPRTIRLTWRPSAGATQYLVRCLPASPAGQEEGREVRVGQPEVLLDGLEPGRDYDVRVQSLQGTEASEARGVRARTSTLAPPRHLGFSDVSHDSARVFWDGLPRPVRLFRVSFFSSDGSHSGQVEVPGNATSASLGPLSSSTTYAVRVTCLYPGGGSSTLTGHLTTRKVPSPSQLVVTRLPGDEARLQWAAASGVLVYQIKWTPLGDGKAHEVSVPGSRAMAVLPGLRSHLEYDITILAYYRDGARSDPVSLRYSPPRRSPPSDLTLASESPNSLRVSWTPPSGPVLHYRLTYALASGSGPEKSISVPGPRSHVTLPDLLAATKYRVLVSAVYGAGESPTVSATGRTAACPALSPDSPLPGFDLMRAFGLVEKEYASVRGVAMEPSAFGRARTFTILRDAQLSRRASAPHLAAPPPEHTVVFLLRVLPETPRETFALWQMTADDFQPVLGVLLDAGRRSLTYFHHDRTAALQEVTFDLPEVKRIFFGSFHKVHVAVGRSKVGLYVDCRKVAERPIGEAGSPPAAGFMMLGRLAKARGPRSSSASFQLQMLQIVCNDSWADEDVCCELPASKDGETCPAFASACTCASQTPALPGPQGPPGLPGRNGAPGERGFPGPRGEPGPPGQMGPEGPGGQQGSPGTQGRTVQGPVGPPGVKGEKGDHGLPGLQGHPGHQGAPGKVGLQGPKGMRGLEGSAGLPGPPGPRGFQGMAGARGTAGERGPPGAVGPTGLPGPKGERGEKGEPQSLATISQLLSRVCESAIQTHVLKLHSFLHESARPPRPILEAPKLPGRGGQDSPRPEDRGEPRTLGPMGSPRPKGSEPPRPLTGTGEHPPRGGETGGAAGWGCSTDKHEAAEKQGLWLAGHRGVQGPWGRLASPWADVGEPGGPMVVVSASGG

Target ORF information:

RefSeq Version	XM_014106882.1
Organism	Canis lupus familiaris(Dog)
Definition	Canis lupus familiaris collagen, type XX, alpha 1 (COL20A1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_014106882.1

ORF Insert Sequence:

ATGACTGCCC CCGCCGGCCC CCGGCTCGGG CTCCGCCTCT GCCTGTGGCT TGGTGCTGCC 
CTGGGACTCG GCCAGGGACC AGCGAGCGGC CGCCTGAGGC TGGCTGTGCT GCCTGAGGAC 
CGGCTACAGA TGAAGTGGAG GGAGTCGGAG GGGAGCAGCC TTGGCTACCT GGTCCAGGTG 
AAGCCCATGG CAGGGGACGC GGAACAGGAG GTGATGCTGA CCACCAAGAC CCCCAAGGCC 
ACGGTGGGGG GCCTGAGCCC CTCCAAGGGC TACACCGTGC AGATCTTCGA GCTCACCGGC 
TCAGGGAACG CCCTGCTGGC GCAGAGGGAG TTTGTGATCG AGGATTTGAA GAGTCACTCT 
GGGAGCAGGA GCGACCAGAA GCCCCTGGGG GCCGCCCCGG AGCCCACGCC CCCCGACGTG 
GGGTGCCCGG ACCCTGAGCC CCTGGGCACC TCGTCCCCAA GCCAAGACCC GCCCACCCTC 
GCTGGTTCAG ACTGGGGCAA GGGTAAGTCC GGCAGGCGGA GTGACCCCCG CGTCTCAAGG 
TTTCACGGGT TCACTGTCCT GAGTCGCTCA GCCTCGTGGG GGCAGGTCAC CCGCTGGGGG 
TGTGCTCGGC CCGTGCCCCT TGCCTCCCAG CCCGCGGGTG GCAGCTCACC CGCCGCCATG 
AGCCCCGCGG CCCCTCAGCC ACCACCAACC CCGGGGAAGG GGATCCAGGG GCTTTGGGAG 
GGAGGTCGCG CGGTGACCAG TGACGTGGGC CTGGCCCAGC TGCTCACCAC CCTCCCTGCC 
CGCCCAGCCG GACCCCAGTT CCGCTGCACA CCCCCCAGCC CCGTGGACAT GATCTTCCTG 
GTGGACGGGT CCTGGAGCAT CGGCCACAGT CACTTCCAGC AGGTCAAAGA TTTCCTGGCC 
AGCGTCATCG AGCCCTTTGA AATCGGGCCA AGTAAAGTCC AAGTCGGCTT GACTCAGTAC 
AGCGGAGACC CCCAGACCGA GTGGGACCTC AACGCCCTCC GCACCAAGGA GGACGTGCTG 
GCAGCTGTCC GCCGTCTGCG CTACAAAGGG GGAAACACGT TCACCGGCCT GGCGCTGACG 
CACGTGCTCG AGCACAACCT GCGGCCCGCA GCGGGGCCCC GGCCTGAGGC CACCAAGGTG 
CTGATCCTGG TGACAGACGG CAAATCCCAG GACGATGCCC GCGCCGCTGG CCGCATCCTC 
AAGGACCTGG GTGTTGCCAT CTTTGCTGTG GGTGTGAAGA ACGCGGACGA GGCTGAGCTG 
CAGCTCCTGG CGTCCCAGCC ACTGGACATC ACCGTCCACA ACGTGCAGGA CTTCCCGCAG 
CTCGGCACCT TGGCCGGCTT GCTCGGCCGC CTCATCTGCC AGAAGGTCCA GGGCGGGAGC 
AGGGACCCGG CCCGGCCGGC AGCAGCCACC CCCGCCCTGG ACACCCTCTT CACTCCCAGC 
AGCCTGCTGT TGACCCAGGT GACCTCCTCC AGCGCCCACC TGTCCTGGAC TCCAGCCGCC 
CAGCTGCCCC TCAAGTATCT GATCGTGTGG CGGCCCTCGA GGGGTGGCCC GCCCCGGGAG 
GTGGTGGTGG ACGGGCCCAC CTTGTCCGCG GAGCTGCACG ACCTGGCCGC CGGCACGGAG 
TACTCGGTGT CTGTGATCCC CGTCGGCAAG GCCGGGGTCG GCGAGGGCCT GCGGGGCCTG 
GTGACCACAG CGCCTCTGCC CCCGCCCCAG GCGCTGACCG TGGCTGCGGT GACACCCAGA 
ACCATCCGCC TCACCTGGCG GCCCTCGGCC GGGGCCACAC AGTACCTGGT GCGATGCCTG 
CCAGCCTCCC CTGCGGGCCA GGAGGAGGGC AGAGAGGTGC GGGTGGGGCA GCCCGAGGTG 
CTGCTGGACG GCCTGGAGCC TGGCAGGGAC TACGATGTCC GGGTGCAGAG CTTGCAGGGC 
ACAGAGGCCA GCGAGGCCCG GGGCGTCCGC GCCAGGACCT CCACCCTGGC GCCCCCCAGA 
CACCTGGGCT TCTCGGATGT GAGTCACGAC TCCGCACGCG TGTTCTGGGA CGGCCTCCCG 
AGGCCCGTCC GCCTGTTCAG GGTCAGCTTT TTCTCCAGCG ACGGCAGCCA CTCGGGGCAG 
GTGGAGGTTC CCGGGAATGC CACCTCGGCC TCTCTGGGCC CTCTGTCCTC CTCCACCACG 
TATGCTGTCC GTGTCACCTG CCTCTACCCT GGGGGCGGCT CCTCCACGTT GACCGGCCAC 
CTGACCACAC GGAAGGTCCC CAGCCCCAGC CAGCTCGTGG TGACCAGGCT CCCCGGGGAC 
GAGGCTCGGC TGCAGTGGGC GGCAGCTTCC GGAGTGCTCG TCTATCAGAT CAAGTGGACA 
CCCCTGGGAG ACGGGAAGGC CCACGAGGTC TCTGTCCCCG GGAGCCGGGC CATGGCAGTC 
CTACCTGGCC TGCGAAGCCA CCTGGAGTAC GACATCACCA TCCTGGCCTA CTACAGGGAC 
GGGGCCCGCA GCGACCCGGT GTCCCTGCGC TACAGCCCCC CGCGCCGCAG CCCGCCGTCT 
GACCTGACCC TGGCTTCCGA GTCGCCCAAC AGCCTGCGAG TCAGCTGGAC GCCCCCGAGC 
GGCCCCGTCC TCCACTACCG GCTCACCTAT GCGCTGGCCT CGGGCTCGGG ACCCGAGAAG 
TCGATCTCTG TCCCAGGACC CAGGAGCCAC GTGACACTGC CCGACCTGCT GGCAGCCACC 
AAATACAGGG TCCTGGTCTC GGCCGTCTAC GGAGCAGGGG AGAGTCCGAC AGTGTCTGCC 
ACAGGCCGGA CCGCAGCCTG CCCTGCGCTC AGCCCTGACA GCCCCCTCCC AGGCTTCGAC 
CTCATGAGGG CCTTCGGGCT GGTGGAGAAG GAGTACGCCT CCGTCCGCGG CGTCGCCATG 
GAGCCCTCGG CTTTCGGCCG CGCCAGGACC TTCACCATCC TCAGGGACGC CCAGCTGAGC 
CGCCGGGCCA GTGCCCCCCA CCTGGCTGCG CCCCCGCCCG AGCACACGGT GGTCTTCCTC 
CTGCGTGTGC TGCCCGAGAC CCCCCGGGAG ACCTTTGCGC TGTGGCAGAT GACGGCCGAC 
GACTTCCAGC CCGTCCTGGG CGTCCTGCTG GACGCGGGCA GGAGGTCTCT GACCTACTTC 
CACCACGACC GCACGGCCGC CTTGCAAGAG GTCACCTTTG ACCTGCCCGA AGTGAAGAGG 
ATATTCTTCG GAAGCTTCCA CAAGGTGCAC GTGGCCGTGG GCCGCTCCAA GGTCGGGCTG 
TACGTGGACT GCAGGAAGGT GGCCGAGAGG CCCATCGGGG AGGCGGGCAG CCCGCCTGCC 
GCCGGCTTCA TGATGCTGGG GAGGCTGGCC AAGGCGCGCG GGCCCCGGAG CAGCTCCGCC 
TCATTCCAGC TCCAGATGCT GCAGATCGTG TGCAATGACT CCTGGGCAGA CGAAGACGTG 
TGCTGCGAGC TCCCTGCGTC GAAGGACGGA GAGACCTGCC CGGCCTTCGC GTCTGCCTGC 
ACCTGCGCCT CGCAGACCCC CGCGCTCCCG GGGCCCCAAG GACCTCCGGG CCTCCCTGGA 
AGGAACGGAG CCCCAGGAGA GCGGGGCTTC CCGGGGCCCA GGGGAGAGCC TGGGCCACCC 
GGACAGATGG GACCAGAGGG TCCTGGAGGT CAGCAGGGCT CGCCAGGGAC CCAGGGCCGG 
ACTGTGCAGG GACCTGTGGG CCCGCCGGGG GTCAAAGGAG AGAAGGGGGA CCATGGGCTC 
CCGGGCCTGC AGGGCCACCC CGGTCACCAG GGCGCCCCCG GGAAGGTCGG CCTCCAGGGA 
CCAAAGGGAA TGAGAGGCCT GGAGGGGAGC GCTGGCCTGC CTGGACCCCC CGGCCCGAGG 
GGCTTCCAGG GCATGGCGGG AGCCAGGGGC ACCGCAGGGG AGAGAGGCCC CCCGGGGGCC 
GTGGGGCCCA CGGGGCTGCC GGGACCCAAA GGGGAGCGAG GAGAGAAGGG CGAGCCACAG 
TCCCTGGCCA CCATCTCCCA GCTGCTGAGC CGGGTCTGCG AGTCTGCCAT CCAGACTCAC 
GTGCTGAAGC TCCACTCCTT CCTCCACGAG AGCGCCAGGC CCCCCCGGCC CATCTTGGAG 
GCGCCCAAGC TCCCTGGACG AGGAGGACAG GACAGTCCCC GCCCCGAGGA CAGAGGCGAG 
CCCAGAACCC TCGGTCCCAT GGGCAGCCCT CGGCCGAAAG GGAGCGAGCC TCCAAGACCC 
CTGACAGGAA CGGGTGAGCA CCCACCCAGG GGAGGGGAGA CTGGGGGGGC TGCAGGGTGG 
GGGTGCAGCA CGGATAAACA CGAAGCTGCC GAGAAGCAGG GCCTCTGGCT GGCTGGGCAC 
AGGGGGGTTC AGGGGCCTTG GGGGAGACTT GCAAGCCCGT GGGCAGATGT GGGGGAGCCA 
GGAGGGCCCA TGGTGGTTGT TAGCGCATCA GGAGGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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COL20A1 cDNA ORF clone, Canis lupus familiaris(dog)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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