AFDN cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene
Clones
gRNAs

Summary

Gene Symbol	AFDN
Entrez Gene ID	463135
Full Name	afadin, adherens junction formation factor
General protein information	Preferred Names afadin, adherens junction formation factor Names afadin myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 4 myeloid/lymphoid or mixed-lineage leukemia ; translocated to, 4
Gene Type	protein-coding
Organism	Pan troglodytes(chimpanzee)

mRNA and Protein(s)

mRNA	Protein	Name
XM_016956636.1	XP_016812125.1	afadin isoform X5
XM_016956655.1	XP_016812144.1	afadin isoform X24
XM_016956634.1	XP_016812123.1	afadin isoform X3
XM_016956656.1	XP_016812145.1	afadin isoform X25
XM_016956644.2	XP_016812133.2	afadin isoform X13
XM_016956645.2	XP_016812134.2	afadin isoform X14
XM_016956647.2	XP_016812136.2	afadin isoform X16
XM_016956653.1	XP_016812142.1	afadin isoform X22
XM_016956654.1	XP_016812143.1	afadin isoform X23
XM_016956635.1	XP_016812124.1	afadin isoform X4
XM_016956650.1	XP_016812139.1	afadin isoform X19
XM_016956633.2	XP_016812122.2	afadin isoform X2
XM_016956649.2	XP_016812138.1	afadin isoform X18
XM_024357512.1	XP_024213280.1	afadin isoform X21
XM_024357513.1	XP_024213281.1	afadin isoform X22
XM_016956652.2	XP_016812141.1	afadin isoform X23
XM_016956653.2	XP_016812142.1	afadin isoform X24
XM_016956652.1	XP_016812141.1	afadin isoform X21
XM_016956645.1	XP_016812134.1	afadin isoform X14
XM_016956647.1	XP_016812136.1	afadin isoform X16
XM_016956643.1	XP_016812132.1	afadin isoform X12
XM_016956638.1	XP_016812127.1	afadin isoform X7
XM_016956640.1	XP_016812129.1	afadin isoform X9
XM_016956649.1	XP_016812138.1	afadin isoform X18
XM_016956638.2	XP_016812127.2	afadin isoform X7
XM_016956640.2	XP_016812129.2	afadin isoform X9
XM_016956651.2	XP_016812140.1	afadin isoform X20
XM_016956656.2	XP_016812145.1	afadin isoform X25
XM_016956639.1	XP_016812128.1	afadin isoform X8
XM_016956641.1	XP_016812130.1	afadin isoform X10
XM_016956642.1	XP_016812131.1	afadin isoform X11
XM_016956646.1	XP_016812135.1	afadin isoform X15
XM_016956651.1	XP_016812140.1	afadin isoform X20
XM_016956632.2	XP_016812121.2	afadin isoform X1
XM_016956636.2	XP_016812125.2	afadin isoform X5
XM_016956639.2	XP_016812128.2	afadin isoform X8
XM_016956641.2	XP_016812130.2	afadin isoform X10
XM_016956643.2	XP_016812132.2	afadin isoform X12
XM_016956646.2	XP_016812135.2	afadin isoform X15
XM_016956648.2	XP_016812137.1	afadin isoform X17
XM_016956632.1	XP_016812121.1	afadin isoform X1
XM_016956633.1	XP_016812122.1	afadin isoform X2
XM_016956637.1	XP_016812126.1	afadin isoform X6
XM_016956644.1	XP_016812133.1	afadin isoform X13
XM_016956648.1	XP_016812137.1	afadin isoform X17
XM_016956634.2	XP_016812123.2	afadin isoform X3
XM_016956635.2	XP_016812124.2	afadin isoform X4
XM_016956637.2	XP_016812126.2	afadin isoform X6
XM_016956642.2	XP_016812131.2	afadin isoform X11
XM_016956650.2	XP_016812139.1	afadin isoform X19

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	afd-1	NP_001021660.1
Gallus gallus (chicken)	MLLT4	XP_004935593.1
Danio rerio (zebrafish)	mllt4	XP_686655.5
Homo sapiens (human)	MLLT4	NP_001193937.1
Pan troglodytes (chimpanzee)	MLLT4	XP_518857.4
	MLLT4	XP_001083153.1
Canis lupus familiaris (dog)	MLLT4	XP_005615632.1
Bos taurus (cattle)	MLLT4	XP_005211198.1
Mus musculus (house mouse)	Mllt4	NP_034936.1
Rattus norvegicus (Norway rat)	Mllt4	NP_037349.1
Xenopus tropicalis (tropical clawed frog)	mllt4	XP_002931548.2

Gene Ontology

The following AFDN gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the AFDN cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OPc107537	XM_016956632.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107538	XM_016956633.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107539	XM_016956634.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107540	XM_016956635.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107541	XM_016956636.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107542	XM_016956637.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107543	XM_016956638.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107544	XM_016956639.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107545	XM_016956640.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107546	XM_016956641.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107547	XM_016956642.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107548	XM_016956643.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107549	XM_016956644.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107550	XM_016956645.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107551	XM_016956646.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107552	XM_016956647.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107553	XM_024357512.1 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc107554	XM_024357513.1 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
OPc87364	XM_016956652.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc87365	XM_016956653.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc87366	XM_016956654.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OPc87368	XM_016956656.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc87344	XM_016956632.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87345	XM_016956633.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87346	XM_016956634.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87347	XM_016956635.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87348	XM_016956636.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87349	XM_016956637.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87350	XM_016956638.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87351	XM_016956639.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87352	XM_016956640.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87353	XM_016956641.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87354	XM_016956642.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87355	XM_016956643.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87356	XM_016956644.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87357	XM_016956645.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87358	XM_016956646.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87359	XM_016956647.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87360	XM_016956648.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87361	XM_016956649.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87362	XM_016956650.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87363	XM_016956651.2 Latest version!	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87367	XM_016956655.1 Latest version!	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$881.30 $1259.00
View Old Accession Versions >>
OPc87365	XM_016956653.1	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc87364	XM_016956652.1	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc87361	XM_016956649.1	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87368	XM_016956656.1	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
OPc87363	XM_016956651.1	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87360	XM_016956648.1	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
OPc87362	XM_016956650.1	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

AFDN ( XM_016956632.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812121.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

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CloneID	OPc107537
Clone ID Related Accession (Same CDS sequence)	XM_016956632.2
Accession Version	XM_016956632.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956632.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACGAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGTAGAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGAGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812121.2
CDS	258..6023
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956632.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956632.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACGAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGTAGAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGAGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956633.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812122.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

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CloneID	OPc107538
Clone ID Related Accession (Same CDS sequence)	XM_016956633.2
Accession Version	XM_016956633.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956633.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACGAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812122.2
CDS	258..6020
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956633.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956633.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACGAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956634.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812123.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

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CloneID	OPc107539
Clone ID Related Accession (Same CDS sequence)	XM_016956634.2
Accession Version	XM_016956634.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956634.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACGAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812123.2
CDS	259..6021
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956634.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956634.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACGAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956635.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812124.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

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CloneID	OPc107540
Clone ID Related Accession (Same CDS sequence)	XM_016956635.2
Accession Version	XM_016956635.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956635.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACGAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812124.2
CDS	258..6020
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956635.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956635.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACGAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956636.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812125.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

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CloneID	OPc107541
Clone ID Related Accession (Same CDS sequence)	XM_016956636.2
Accession Version	XM_016956636.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956636.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACGAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812125.2
CDS	258..6017
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956636.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956636.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACGAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956637.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812126.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

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CloneID	OPc107542
Clone ID Related Accession (Same CDS sequence)	XM_016956637.2
Accession Version	XM_016956637.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956637.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812126.2
CDS	258..6017
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956637.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956637.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956638.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812127.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

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CloneID	OPc107543
Clone ID Related Accession (Same CDS sequence)	XM_016956638.2
Accession Version	XM_016956638.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956638.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGTTT CTGGGCCTGA ACTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGCGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT 
GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAAAGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC 
TGGGGTGCAA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG 
CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCACTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC 
CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT 
TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812127.2
CDS	259..6018
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956638.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956638.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGTTT CTGGGCCTGA ACTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGCGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT 
GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAAAGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC 
TGGGGTGCAA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG 
CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCACTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC 
CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT 
TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956639.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812128.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

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CloneID	OPc107544
Clone ID Related Accession (Same CDS sequence)	XM_016956639.2
Accession Version	XM_016956639.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956639.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AACCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAGTCTC ATGGGGACCT TCTTCCTGCC 
CCTCTTAAAC CACGGCCACC CCCCTGCCAG CCACGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCAGCCAAA GCCAACAGTA CTGCTCACGA AAAAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ATCCAGCTCC TACCATCCCA GCCACTGCAA AGGTAGAGGA 
CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGAGAT 
GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT 
GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT 
AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812128.2
CDS	259..6015
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARTAMPAISVLDLDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956639.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956639.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AACCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAGTCTC ATGGGGACCT TCTTCCTGCC 
CCTCTTAAAC CACGGCCACC CCCCTGCCAG CCACGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCAGCCAAA GCCAACAGTA CTGCTCACGA AAAAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ATCCAGCTCC TACCATCCCA GCCACTGCAA AGGTAGAGGA 
CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGAGAT 
GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT 
GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT 
AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956640.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812129.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

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CloneID	OPc107545
Clone ID Related Accession (Same CDS sequence)	XM_016956640.2
Accession Version	XM_016956640.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956640.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAA CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGTCAG 
GATAAGTACT CCAGCACAAG AAAGTCTCAT GGGGACCTTC TTCCTGCCCC TCTTAAACCA 
CGGCCACCCC CCTGCCAGCC ACGCCCGGCC TCCGATGGCA TCTTTCTCTC CAACTCATTC 
CAGCCACCCT CAGCCAAAGC CAACAGTACT GCTCACGAAA AAGGCCAGCC CCCGCCCCCT 
CCAAAAAAAT CCAGCTCCTA CCATCCCAGC CACTGCAAAG GTAGAGGACC AAACTCTTAC 
CCAGGATCTA CTGGAGCAGC TGTTGGAGCC CATGACGCCT GTCGAGATGC AAAAGAGAAG 
CGCTCTAAAA GCCAAGATGC AGATTCACCT GGAAGTTCTG GGGCCCCTGA AAACTTGACA 
TTCAAGGAAC GCCAGCGTCT TTTTTCACAA GGTCAAGATG TATCCAATAA AGTGAAAGCT 
TCTCGTAAAT TAACAGAACT GGAGAATGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812129.2
CDS	258..6002
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956640.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956640.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAA CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGTCAG 
GATAAGTACT CCAGCACAAG AAAGTCTCAT GGGGACCTTC TTCCTGCCCC TCTTAAACCA 
CGGCCACCCC CCTGCCAGCC ACGCCCGGCC TCCGATGGCA TCTTTCTCTC CAACTCATTC 
CAGCCACCCT CAGCCAAAGC CAACAGTACT GCTCACGAAA AAGGCCAGCC CCCGCCCCCT 
CCAAAAAAAT CCAGCTCCTA CCATCCCAGC CACTGCAAAG GTAGAGGACC AAACTCTTAC 
CCAGGATCTA CTGGAGCAGC TGTTGGAGCC CATGACGCCT GTCGAGATGC AAAAGAGAAG 
CGCTCTAAAA GCCAAGATGC AGATTCACCT GGAAGTTCTG GGGCCCCTGA AAACTTGACA 
TTCAAGGAAC GCCAGCGTCT TTTTTCACAA GGTCAAGATG TATCCAATAA AGTGAAAGCT 
TCTCGTAAAT TAACAGAACT GGAGAATGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956641.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812130.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

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CloneID	OPc107546
Clone ID Related Accession (Same CDS sequence)	XM_016956641.2
Accession Version	XM_016956641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG 
AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GACGAGGCGG CGCGCAGGTT GCTGGAACCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT 
CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT 
CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC 
AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGTCAGGAT 
AAGTACTCCA GCACAAGAAA GTCTCATGGG GACCTTCTTC CTGCCCCTCT TAAACCACGG 
CCACCCCCCT GCCAGCCACG CCCGGCCTCC GATGGCATCT TTCTCTCCAA CTCATTCCAG 
CCACCCTCAG CCAAAGCCAA CAGTACTGCT CACGAAAAAG GCCAGCCCCC GCCCCCTCCA 
AAAAAATCCA GCTCCTACCA TCCCAGCCAC TGCAAAGGTA GAGGACCAAA CTCTTACCCA 
GGATCTACTG GAGCAGCTGT TGGAGCCCAT GACGCCTGTC GAGATGCAAA AGAGAAGCGC 
TCTAAAAGCC AAGATGCAGA TTCACCTGGA AGTTCTGGGG CCCCTGAAAA CTTGACATTC 
AAGGAACGCC AGCGTCTTTT TTCACAAGGT CAAGATGTAT CCAATAAAGT GAAAGCTTCT 
CGTAAATTAA CAGAACTGGA GAATGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812130.2
CDS	258..5999
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956641.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956641.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG 
AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GACGAGGCGG CGCGCAGGTT GCTGGAACCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT 
CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT 
CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC 
AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGTCAGGAT 
AAGTACTCCA GCACAAGAAA GTCTCATGGG GACCTTCTTC CTGCCCCTCT TAAACCACGG 
CCACCCCCCT GCCAGCCACG CCCGGCCTCC GATGGCATCT TTCTCTCCAA CTCATTCCAG 
CCACCCTCAG CCAAAGCCAA CAGTACTGCT CACGAAAAAG GCCAGCCCCC GCCCCCTCCA 
AAAAAATCCA GCTCCTACCA TCCCAGCCAC TGCAAAGGTA GAGGACCAAA CTCTTACCCA 
GGATCTACTG GAGCAGCTGT TGGAGCCCAT GACGCCTGTC GAGATGCAAA AGAGAAGCGC 
TCTAAAAGCC AAGATGCAGA TTCACCTGGA AGTTCTGGGG CCCCTGAAAA CTTGACATTC 
AAGGAACGCC AGCGTCTTTT TTCACAAGGT CAAGATGTAT CCAATAAAGT GAAAGCTTCT 
CGTAAATTAA CAGAACTGGA GAATGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956642.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812131.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

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CloneID	OPc107547
Clone ID Related Accession (Same CDS sequence)	XM_016956642.2
Accession Version	XM_016956642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC 
GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC 
TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA 
TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GTCAGGATAA GTACTCCAGC 
ACAAGAAAGT CTCATGGGGA CCTTCTTCCT GCCCCTCTTA AACCACGGCC ACCCCCCTGC 
CAGCCACGCC CGGCCTCCGA TGGCATCTTT CTCTCCAACT CATTCCAGCC ACCCTCAGCC 
AAAGCCAACA GTACTGCTCA CGAAAAAGGC CAGCCCCCGC CCCCTCCAAA AAAATCCAGC 
TCCTACCATC CCAGCCACTG CAAAGGTAGA GGACCAAACT CTTACCCAGG ATCTACTGGA 
GCAGCTGTTG GAGCCCATGA CGCCTGTCGA GATGCAAAAG AGAAGCGCTC TAAAAGCCAA 
GATGCAGATT CACCTGGAAG TTCTGGGGCC CCTGAAAACT TGACATTCAA GGAACGCCAG 
CGTCTTTTTT CACAAGGTCA AGATGTATCC AATAAAGTGA AAGCTTCTCG TAAATTAACA 
GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812131.2
CDS	257..5986
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956642.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956642.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC 
GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC 
TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA 
TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GTCAGGATAA GTACTCCAGC 
ACAAGAAAGT CTCATGGGGA CCTTCTTCCT GCCCCTCTTA AACCACGGCC ACCCCCCTGC 
CAGCCACGCC CGGCCTCCGA TGGCATCTTT CTCTCCAACT CATTCCAGCC ACCCTCAGCC 
AAAGCCAACA GTACTGCTCA CGAAAAAGGC CAGCCCCCGC CCCCTCCAAA AAAATCCAGC 
TCCTACCATC CCAGCCACTG CAAAGGTAGA GGACCAAACT CTTACCCAGG ATCTACTGGA 
GCAGCTGTTG GAGCCCATGA CGCCTGTCGA GATGCAAAAG AGAAGCGCTC TAAAAGCCAA 
GATGCAGATT CACCTGGAAG TTCTGGGGCC CCTGAAAACT TGACATTCAA GGAACGCCAG 
CGTCTTTTTT CACAAGGTCA AGATGTATCC AATAAAGTGA AAGCTTCTCG TAAATTAACA 
GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956643.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812132.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

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CloneID	OPc107548
Clone ID Related Accession (Same CDS sequence)	XM_016956643.2
Accession Version	XM_016956643.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956643.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGTCAGG ATAAGTACTC CAGCACAAGA 
AAGTCTCATG GGGACCTTCT TCCTGCCCCT CTTAAACCAC GGCCACCCCC CTGCCAGCCA 
CGCCCGGCCT CCGATGGCAT CTTTCTCTCC AACTCATTCC AGCCACCCTC AGCCAAAGCC 
AACAGTACTG CTCACGAAAA AGGCCAGCCC CCGCCCCCTC CAAAAAAATC CAGCTCCTAC 
CATCCCAGCC ACTGCAAAGG TAGAGGACCA AACTCTTACC CAGGATCTAC TGGAGCAGCT 
GTTGGAGCCC ATGACGCCTG TCGAGATGCA AAAGAGAAGC GCTCTAAAAG CCAAGATGCA 
GATTCACCTG GAAGTTCTGG GGCCCCTGAA AACTTGACAT TCAAGGAACG CCAGCGTCTT 
TTTTCACAAG GTCAAGATGT ATCCAATAAA GTGAAAGCTT CTCGTAAATT AACAGAACTG 
GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812132.2
CDS	258..5981
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956643.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956643.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGTCAGG ATAAGTACTC CAGCACAAGA 
AAGTCTCATG GGGACCTTCT TCCTGCCCCT CTTAAACCAC GGCCACCCCC CTGCCAGCCA 
CGCCCGGCCT CCGATGGCAT CTTTCTCTCC AACTCATTCC AGCCACCCTC AGCCAAAGCC 
AACAGTACTG CTCACGAAAA AGGCCAGCCC CCGCCCCCTC CAAAAAAATC CAGCTCCTAC 
CATCCCAGCC ACTGCAAAGG TAGAGGACCA AACTCTTACC CAGGATCTAC TGGAGCAGCT 
GTTGGAGCCC ATGACGCCTG TCGAGATGCA AAAGAGAAGC GCTCTAAAAG CCAAGATGCA 
GATTCACCTG GAAGTTCTGG GGCCCCTGAA AACTTGACAT TCAAGGAACG CCAGCGTCTT 
TTTTCACAAG GTCAAGATGT ATCCAATAAA GTGAAAGCTT CTCGTAAATT AACAGAACTG 
GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956644.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812133.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

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CloneID	OPc107549
Clone ID Related Accession (Same CDS sequence)	XM_016956644.2
Accession Version	XM_016956644.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956644.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGTT 
TCTGGGCCTG AACTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGCGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCA ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCACTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCGCAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAAGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCTAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCATCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCAC CAACCAGATA GGGCTGCCGT CTGCACAGGT GGCTGCTGCC 
GAACGGAAAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATCACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGAGGACGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGACGAGG AGCGGAGGCG GCAGCAGCAG 
TTAGAAGAGA TGCGCAAGCG GGAAGCGGAA GACCGAGCGA GGCAAGAGGA AGAGCGCCGG 
CGGCAGGAGG AGGAGCGAAC AAAACGAGAC GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG ACGAGGCGGC GCGCAGGTTG 
CTGGAACCCG AGGCGCCCGG TCTGTGCCGC CCTCCGCTTC CCCGGGACTA CGAGCCCCCG 
TCCCCGTCCC CCGCGCCCGG CGCCCCTCCT CCCCCGCCTC AGCGAAACGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC CGACTCGCTG TTCACTGCCA AGTTTGTTGC ATACAATGAG 
GAGGAGGAGG AGGAGGACTG CAGCCTAGCA GGTCAGGATA AGTACTCCAG CACAAGAAAG 
TCTCATGGGG ACCTTCTTCC TGCCCCTCTT AAACCACGGC CACCCCCCTG CCAGCCACGC 
CCGGCCTCCG ATGGCATCTT TCTCTCCAAC TCATTCCAGC CACCCTCAGC CAAAGCCAAC 
AGTACTGCTC ACGAAAAAGG CCAGCCCCCG CCCCCTCCAA AAAAATCCAG CTCCTACCAT 
CCCAGCCACT GCAAAGGTAG AGGACCAAAC TCTTACCCAG GATCTACTGG AGCAGCTGTT 
GGAGCCCATG ACGCCTGTCG AGATGCAAAA GAGAAGCGCT CTAAAAGCCA AGATGCAGAT 
TCACCTGGAA GTTCTGGGGC CCCTGAAAAC TTGACATTCA AGGAACGCCA GCGTCTTTTT 
TCACAAGGTC AAGATGTATC CAATAAAGTG AAAGCTTCTC GTAAATTAAC AGAACTGGAG 
AATGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812133.2
CDS	258..5978
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956644.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956644.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGTT 
TCTGGGCCTG AACTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGCGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCA ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCACTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCGCAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAAGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCTAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCATCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCAC CAACCAGATA GGGCTGCCGT CTGCACAGGT GGCTGCTGCC 
GAACGGAAAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATCACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGAGGACGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGACGAGG AGCGGAGGCG GCAGCAGCAG 
TTAGAAGAGA TGCGCAAGCG GGAAGCGGAA GACCGAGCGA GGCAAGAGGA AGAGCGCCGG 
CGGCAGGAGG AGGAGCGAAC AAAACGAGAC GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG ACGAGGCGGC GCGCAGGTTG 
CTGGAACCCG AGGCGCCCGG TCTGTGCCGC CCTCCGCTTC CCCGGGACTA CGAGCCCCCG 
TCCCCGTCCC CCGCGCCCGG CGCCCCTCCT CCCCCGCCTC AGCGAAACGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC CGACTCGCTG TTCACTGCCA AGTTTGTTGC ATACAATGAG 
GAGGAGGAGG AGGAGGACTG CAGCCTAGCA GGTCAGGATA AGTACTCCAG CACAAGAAAG 
TCTCATGGGG ACCTTCTTCC TGCCCCTCTT AAACCACGGC CACCCCCCTG CCAGCCACGC 
CCGGCCTCCG ATGGCATCTT TCTCTCCAAC TCATTCCAGC CACCCTCAGC CAAAGCCAAC 
AGTACTGCTC ACGAAAAAGG CCAGCCCCCG CCCCCTCCAA AAAAATCCAG CTCCTACCAT 
CCCAGCCACT GCAAAGGTAG AGGACCAAAC TCTTACCCAG GATCTACTGG AGCAGCTGTT 
GGAGCCCATG ACGCCTGTCG AGATGCAAAA GAGAAGCGCT CTAAAAGCCA AGATGCAGAT 
TCACCTGGAA GTTCTGGGGC CCCTGAAAAC TTGACATTCA AGGAACGCCA GCGTCTTTTT 
TCACAAGGTC AAGATGTATC CAATAAAGTG AAAGCTTCTC GTAAATTAAC AGAACTGGAG 
AATGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956645.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812134.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

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CloneID	OPc107550
Clone ID Related Accession (Same CDS sequence)	XM_016956645.2
Accession Version	XM_016956645.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956645.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812134.2
CDS	258..5957
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956645.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956645.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956646.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812135.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

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CloneID	OPc107551
Clone ID Related Accession (Same CDS sequence)	XM_016956646.2
Accession Version	XM_016956646.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956646.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812135.2
CDS	259..5958
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956646.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956646.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CGAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956647.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812136.2 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

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CloneID	OPc107552
Clone ID Related Accession (Same CDS sequence)	XM_016956647.2
Accession Version	XM_016956647.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956647.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGAG GCGGCAGCAG CAGTTAGAAG AGATGCGCAA GCGGGAAGCG 
GAAGACCGAG CGAGGCAAGA GGAAGAGCGC CGGCGGCAGG AGGAGGAGCG AACAAAACGA 
GACGCTGAAG AAAAGAGGCG ACAGGAAGAA GGGTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGACGAGGC GGCGCGCAGG TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC 
CGCCCTCCGC TTCCCCGGGA CTACGAGCCC CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT 
CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC CTCAAAACAC AGGTCCTCTC CCCCGACTCG 
CTGTTCACTG CCAAGTTTGT TGCATACAAT GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA 
GCAGGTCAGG ATAAGTACTC CAGCACAAGA AAGTCTCATG GGGACCTTCT TCCTGCCCCT 
CTTAAACCAC GGCCACCCCC CTGCCAGCCA CGCCCGGCCT CCGATGGCAT CTTTCTCTCC 
AACTCATTCC AGCCACCCTC AGCCAAAGCC AACAGTACTG CTCACGAAAA AGGCCAGCCC 
CCGCCCCCTC CAAAAAAATC CAGCTCCTAC CATCCCAGCC ACTGCAAAGG TAGAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGAGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812136.2
CDS	258..5951
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHEKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956647.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956647.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGAG GCGGCAGCAG CAGTTAGAAG AGATGCGCAA GCGGGAAGCG 
GAAGACCGAG CGAGGCAAGA GGAAGAGCGC CGGCGGCAGG AGGAGGAGCG AACAAAACGA 
GACGCTGAAG AAAAGAGGCG ACAGGAAGAA GGGTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGACGAGGC GGCGCGCAGG TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC 
CGCCCTCCGC TTCCCCGGGA CTACGAGCCC CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT 
CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC CTCAAAACAC AGGTCCTCTC CCCCGACTCG 
CTGTTCACTG CCAAGTTTGT TGCATACAAT GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA 
GCAGGTCAGG ATAAGTACTC CAGCACAAGA AAGTCTCATG GGGACCTTCT TCCTGCCCCT 
CTTAAACCAC GGCCACCCCC CTGCCAGCCA CGCCCGGCCT CCGATGGCAT CTTTCTCTCC 
AACTCATTCC AGCCACCCTC AGCCAAAGCC AACAGTACTG CTCACGAAAA AGGCCAGCCC 
CCGCCCCCTC CAAAAAAATC CAGCTCCTAC CATCCCAGCC ACTGCAAAGG TAGAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGAGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024357512.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024213280.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X21, mRNA.

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CloneID	OPc107553
Clone ID Related Accession (Same CDS sequence)	XM_024357512.1
Accession Version	XM_024357512.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5469bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GACCAAACTC TTACCCAGGA TCTACTGGAG CAGCTGTTGG AGCCCATGAC 
GCCTGTCGAG ATGCAAAAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATTC ACCTGGAAGT 
TCTGGGGCCC CTGAAAACTT GACATTCAAG GAACGCCAGC GTCTTTTTTC ACAAGGTCAA 
GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024213280.1
CDS	257..5725
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024357512.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024357512.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GACCAAACTC TTACCCAGGA TCTACTGGAG CAGCTGTTGG AGCCCATGAC 
GCCTGTCGAG ATGCAAAAGA GAAGCGCTCT AAAAGCCAAG ATGCAGATTC ACCTGGAAGT 
TCTGGGGCCC CTGAAAACTT GACATTCAAG GAACGCCAGC GTCTTTTTTC ACAAGGTCAA 
GATGTATCCA ATAAAGTGAA AGCTTCTCGT AAATTAACAG AACTGGAGAA TGAACTGAAC 
ACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_024357513.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_024213281.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X22, mRNA.

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CloneID	OPc107554
Clone ID Related Accession (Same CDS sequence)	XM_024357513.1
Accession Version	XM_024357513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5346bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTAATC GAAACGCTCG CGGAGAAATT TCGACCTGAT 
ATGCGAATGC TGTCGTCTCC CAAGTATTCA CTCTATGAAG TGCATGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCTCTA GTTGTACAAC TGAATTGGAA CAAAGATGAT 
CGGGAAGGCA GATTTGTTCT TAAGAATGAG AATGACGCCA TTCCTCCTAA GAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGTTT CTGGGCCTGA ACTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGCGTCAT CCAGAAACAG 
AAGAATATTG CAGGGGCACT TGCCTTCTGG ATGGCAAATG CATCTGAACT TCTCAACTTC 
ATTAAGCAAG ACCGAGACCT TAGTCGGATC ACACTGGATG CTCAAGATGT TTTAGCACAT 
TTGGTTCAAA TGGCATTTAA ATACTTGGTT CACTGTCTTC AATCAGAACT TAATAATTAC 
ATGCCAGCCT TTCTAGATGA CCCTGAAGAG AACAGTCTGC AAAGACCAAA AATAGATGAT 
GTGCTGCACA CGCTCACAGG AGCCATGTCC TTGCTACGAC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTCATCA ATATGTGGCT GTTCAATAGA 
TTGGTGACCG ACCCAGATTC GGGGCTGTGC TCCCATTACT GGGGTGCAAT TATCCGTCAG 
CAGTTGGGCC ATATTGAAGC CTGGGCTGAG AAGCAGGGGC TGGAACTGGC TGCGGACTGT 
CATCTGAGCA GGATCGTGCA GGCAACGACT TTGCTTACCA TGGATAAGTA TGCACCTGAT 
GACATTCCAA ATATAAACAG CACCTGCTTT AAGTTAAATT CACTACAACT TCAAGCCTTA 
TTACAGAACT ATCACTGTGC ACCTGATGAG CCTTTTATCC CAACGGATCT TATAGAAAAT 
GTAGTGACTG TGGCTGAAAA CACTGCCGAT GAGCTGGCCC GCAGTGATGG AAGGGAAGTG 
CAGTTGGAGG AGGATCCTGA TCTGCAGCTG CCGTTTCTTT TGCCAGAAGA TGGTTATTCT 
TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG 
AGAGGATTTT GCAGGTTAAT TCCTCACACA CGTTCACCAG GTACTTGGAC AATATATTTT 
GAAGGTGCAG ATTATGAAAG TCACCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACC CTAAAAAAGC AGAATGGAAT GGGCCTTAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTAGGAA TCTATGTGAA GTCGGTTGTG 
AAAGGAGGTG CTGCAGATGT GGATGGACGT CTAGCTGCAG GTGATCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTTGG ACTCTCTCAG GAAAGGGCAG CAGAACTCAT GACAAGAACA 
AGCTCTGTGG TGACACTGGA AGTAGCAAAG CAGGGTGCCA TCTACCACGG TCTGGCCACC 
CTTCTCAATC AGCCATCCCC CATGATGCAG AGAATTTCAG ATCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG CTTTGAGCTC TATAATAATT CAACTCAAAA TGGGTCTCCT 
GAGAGTCCTC AGCTGCCTTG GGCAGAATAT AGTGAACCAA AGAAATTGCC TGGTGATGAC 
AGACTGATGA AAAATAGAGC TGATCACCGT TCCAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGTCCTGGAG GGAAAAGTGC ATATGCCTCT GGAACAACAG CGAAGATAAC ATCTGTCTCT 
ACTGGAAACC TCTGCACTGA GGAGCAGACG CCTCCGCCTA GACCTGAAGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTT ACCTTCCCAG CTTCCAAATC CCAGGATCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TATGAGGAAA AGCCGCATAT GCACACAGAT 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACACGTT CCCAAGAAGA ACTTCGAGAA 
GATAAAGCTT ACCAACTTGA GCGGCATCGA ATAGAGGCAG CTATGGACCG AAAGTCTGAT 
AGTGATATGT GGATAAATCA GAGCTCCTCA CTGGACTCCA GTACCTCTAG CCAAGAGCAT 
CTGAACCATT CCTCTAAGTC GGTCACCCCT GCTTCTACAC TGACCAAAAG TGGCCCTGGC 
CGTTGGAAAA CACCAGCAGC CATACCGGCC ACCCCTGTGG CCATCTCCCA GCCAATCCGA 
ACAGACCTGC CTCCGCCACC CCCGCCACCT CCAGTCCACT ATGCCGGTGA TTTCGATGGA 
ATGTCCATGG ATTTGCCTCT CCCACCACCC CCTTCCACCA ACCAGATAGG GCTGCCGTCT 
GCACAGGTGG CTGCTGCCGA ACGGAAAAAG AGAGAAGAAC ATCAGCGTTG GTATGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAG AGGAAGCGGA GAGAGCAGGA GAGGAAGTTG 
GGCCAGATGC GCACTCAGTC CTTAAACCCT GCTCCGTTTT CTCCCCTGAC TGCACAGCAG 
ATGAAGCCCG AAAAGCCTTC CACACTCCAG CGGCCACAGG AAACAGTCAT TCGGGAGCTG 
CAGCCTCAGC AGCAGCCCCG CACGATCGAG CGCAGAGACT TGCAGTACAT CACAGTCAGC 
AAAGAGGAGC TTTCCTCGGG GGACAGTCTG TCCCCCGACC CGTGGAAGCG GGACGCCAAG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC ATCGTGGACA TGCTGAGCAA GGAGATCCAG 
GAGCTCCAGA GCAAACCGGA CCGCAGCGCC GAGGAGAGCG ACCGGCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTCCAG GAGTCGAAGC AGAAGGACGA AGATGACGAG 
GAGGAGGAGG AGGACGATGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCTGAACGA 
AGAGCGAGGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AACCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGAGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024213281.1
CDS	319..5664
Translation	MRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_024357513.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024357513.1

ORF Insert Sequence:

ATGAGATTTT ATTTTCAAGA TAAAGCTGCT GGAAACTTTG CAACAAAATG TATTCGGGTC 
TCTAGTACTG CCACCACTCA AGATGTAATC GAAACGCTCG CGGAGAAATT TCGACCTGAT 
ATGCGAATGC TGTCGTCTCC CAAGTATTCA CTCTATGAAG TGCATGTCAG CGGAGAAGAA 
AGAAGATTGG ATATAGATGA GAAACCTCTA GTTGTACAAC TGAATTGGAA CAAAGATGAT 
CGGGAAGGCA GATTTGTTCT TAAGAATGAG AATGACGCCA TTCCTCCTAA GAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGTTT CTGGGCCTGA ACTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGCGTCAT CCAGAAACAG 
AAGAATATTG CAGGGGCACT TGCCTTCTGG ATGGCAAATG CATCTGAACT TCTCAACTTC 
ATTAAGCAAG ACCGAGACCT TAGTCGGATC ACACTGGATG CTCAAGATGT TTTAGCACAT 
TTGGTTCAAA TGGCATTTAA ATACTTGGTT CACTGTCTTC AATCAGAACT TAATAATTAC 
ATGCCAGCCT TTCTAGATGA CCCTGAAGAG AACAGTCTGC AAAGACCAAA AATAGATGAT 
GTGCTGCACA CGCTCACAGG AGCCATGTCC TTGCTACGAC GCTGCAGAGT CAATGCCGCC 
CTGACCATCC AGCTCTTCTC TCAGCTCTTC CACTTCATCA ATATGTGGCT GTTCAATAGA 
TTGGTGACCG ACCCAGATTC GGGGCTGTGC TCCCATTACT GGGGTGCAAT TATCCGTCAG 
CAGTTGGGCC ATATTGAAGC CTGGGCTGAG AAGCAGGGGC TGGAACTGGC TGCGGACTGT 
CATCTGAGCA GGATCGTGCA GGCAACGACT TTGCTTACCA TGGATAAGTA TGCACCTGAT 
GACATTCCAA ATATAAACAG CACCTGCTTT AAGTTAAATT CACTACAACT TCAAGCCTTA 
TTACAGAACT ATCACTGTGC ACCTGATGAG CCTTTTATCC CAACGGATCT TATAGAAAAT 
GTAGTGACTG TGGCTGAAAA CACTGCCGAT GAGCTGGCCC GCAGTGATGG AAGGGAAGTG 
CAGTTGGAGG AGGATCCTGA TCTGCAGCTG CCGTTTCTTT TGCCAGAAGA TGGTTATTCT 
TGTGATGTTG TCAGAAACAT TCCAAATGGT TTACAAGAAT TTTTAGACCC TCTGTGCCAG 
AGAGGATTTT GCAGGTTAAT TCCTCACACA CGTTCACCAG GTACTTGGAC AATATATTTT 
GAAGGTGCAG ATTATGAAAG TCACCTTCTG CGTGAGAACA CAGAGCTGGC TCAGCCTCTG 
AGGAAAGAAC CTGAAATAAT CACTGTGACC CTAAAAAAGC AGAATGGAAT GGGCCTTAGC 
ATTGTTGCAG CAAAGGGTGC TGGTCAAGAT AAACTAGGAA TCTATGTGAA GTCGGTTGTG 
AAAGGAGGTG CTGCAGATGT GGATGGACGT CTAGCTGCAG GTGATCAGCT CCTCAGTGTG 
GATGGACGAA GTCTGGTTGG ACTCTCTCAG GAAAGGGCAG CAGAACTCAT GACAAGAACA 
AGCTCTGTGG TGACACTGGA AGTAGCAAAG CAGGGTGCCA TCTACCACGG TCTGGCCACC 
CTTCTCAATC AGCCATCCCC CATGATGCAG AGAATTTCAG ATCGTCGTGG CTCAGGTAAA 
CCCCGACCAA AGAGTGAAGG CTTTGAGCTC TATAATAATT CAACTCAAAA TGGGTCTCCT 
GAGAGTCCTC AGCTGCCTTG GGCAGAATAT AGTGAACCAA AGAAATTGCC TGGTGATGAC 
AGACTGATGA AAAATAGAGC TGATCACCGT TCCAGCCCCA ACGTAGCAAA TCAGCCTCCT 
AGTCCTGGAG GGAAAAGTGC ATATGCCTCT GGAACAACAG CGAAGATAAC ATCTGTCTCT 
ACTGGAAACC TCTGCACTGA GGAGCAGACG CCTCCGCCTA GACCTGAAGC CTACCCCATC 
CCCACTCAGA CGTACACCAG AGAGTATTTT ACCTTCCCAG CTTCCAAATC CCAGGATCGG 
ATGGCTCCTC CTCAGAACCA GTGGCCAAAT TATGAGGAAA AGCCGCATAT GCACACAGAT 
AGTAATCATT CCAGTATTGC AATTCAGCGT GTTACACGTT CCCAAGAAGA ACTTCGAGAA 
GATAAAGCTT ACCAACTTGA GCGGCATCGA ATAGAGGCAG CTATGGACCG AAAGTCTGAT 
AGTGATATGT GGATAAATCA GAGCTCCTCA CTGGACTCCA GTACCTCTAG CCAAGAGCAT 
CTGAACCATT CCTCTAAGTC GGTCACCCCT GCTTCTACAC TGACCAAAAG TGGCCCTGGC 
CGTTGGAAAA CACCAGCAGC CATACCGGCC ACCCCTGTGG CCATCTCCCA GCCAATCCGA 
ACAGACCTGC CTCCGCCACC CCCGCCACCT CCAGTCCACT ATGCCGGTGA TTTCGATGGA 
ATGTCCATGG ATTTGCCTCT CCCACCACCC CCTTCCACCA ACCAGATAGG GCTGCCGTCT 
GCACAGGTGG CTGCTGCCGA ACGGAAAAAG AGAGAAGAAC ATCAGCGTTG GTATGAGAAG 
GAGAAGGCCC GCCTGGAGGA GGAGCGGGAG AGGAAGCGGA GAGAGCAGGA GAGGAAGTTG 
GGCCAGATGC GCACTCAGTC CTTAAACCCT GCTCCGTTTT CTCCCCTGAC TGCACAGCAG 
ATGAAGCCCG AAAAGCCTTC CACACTCCAG CGGCCACAGG AAACAGTCAT TCGGGAGCTG 
CAGCCTCAGC AGCAGCCCCG CACGATCGAG CGCAGAGACT TGCAGTACAT CACAGTCAGC 
AAAGAGGAGC TTTCCTCGGG GGACAGTCTG TCCCCCGACC CGTGGAAGCG GGACGCCAAG 
GAGAAGCTGG AGAAGCAGCA GCAGATGCAC ATCGTGGACA TGCTGAGCAA GGAGATCCAG 
GAGCTCCAGA GCAAACCGGA CCGCAGCGCC GAGGAGAGCG ACCGGCTGCG CAAGCTCATG 
CTGGAGTGGC AGTTCCAGAA GAGACTCCAG GAGTCGAAGC AGAAGGACGA AGATGACGAG 
GAGGAGGAGG AGGACGATGT GGACACCATG CTGATCATGC AGCGCCTGGA GGCTGAACGA 
AGAGCGAGGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AACCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGAGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956652.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812141.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X23, mRNA.

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CloneID	OPc87364
Clone ID Related Accession (Same CDS sequence)	XM_016956652.2 , XM_016956652.1
Accession Version	XM_016956652.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956652.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812141.1
CDS	257..5599
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956652.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956652.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956652.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812141.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X21, mRNA.

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CloneID	OPc87364
Clone ID Related Accession (Same CDS sequence)	XM_016956652.2 , XM_016956652.1
Accession Version	XM_016956652.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812141.1
CDS	244..5586
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956652.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956652.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956653.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812142.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X22, mRNA.

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CloneID	OPc87365
Clone ID Related Accession (Same CDS sequence)	XM_016956653.1 , XM_016956653.2
Accession Version	XM_016956653.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGAGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812142.1
CDS	243..5549
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956653.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956653.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGAGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956653.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812142.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X24, mRNA.

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CloneID	OPc87365
Clone ID Related Accession (Same CDS sequence)	XM_016956653.1 , XM_016956653.2
Accession Version	XM_016956653.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956653.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGAGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812142.1
CDS	256..5562
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956653.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956653.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGA CCAAACTCTT ACCCAGGATC TACTGGAGCA 
GCTGTTGGAG CCCATGACGC CTGTCGAGAT GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT 
GCAGATTCAC CTGGAAGTTC TGGGGCCCCT GAAAACTTGA CATTCAAGGA ACGCCAGCGT 
CTTTTTTCAC AAGGTCAAGA TGTATCCAAT AAAGTGAAAG CTTCTCGTAA ATTAACAGAA 
CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956654.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812143.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X23, mRNA.

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CloneID	OPc87366
Clone ID Related Accession (Same CDS sequence)	XM_016956654.1
Accession Version	XM_016956654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4956bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812143.1
CDS	244..5199
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKVMVL

Target ORF information:

RefSeq Version	XM_016956654.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956654.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GGTTATGGTC CTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956656.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812145.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X25, mRNA.

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CloneID	OPc87368
Clone ID Related Accession (Same CDS sequence)	XM_016956656.1 , XM_016956656.2
Accession Version	XM_016956656.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAACCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812145.1
CDS	242..5476
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956656.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956656.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAACCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956656.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812145.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X25, mRNA.

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CloneID	OPc87368
Clone ID Related Accession (Same CDS sequence)	XM_016956656.1 , XM_016956656.2
Accession Version	XM_016956656.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5235bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956656.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAACCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812145.1
CDS	256..5490
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956656.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956656.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGTTGCAG GACGAGGAGC GGAGGCGGCA GCAGCAGTTA 
GAAGAGATGC GCAAGCGGGA AGCGGAAGAC CGAGCGAGGC AAGAGGAAGA GCGCCGGCGG 
CAGGAGGAGG AGCGAACAAA ACGAGACGCT GAAGAAAAGA GGCGACAGGA AGAAGGGTAT 
TACAGCCGCC TGGAAGCCGA GAGGCGCAGA CAGCACGACG AGGCGGCGCG CAGGTTGCTG 
GAACCCGAGG CGCCCGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956632.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812121.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OPc87344
Clone ID Related Accession (Same CDS sequence)	XM_016956632.1
Accession Version	XM_016956632.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACAAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGTAGAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGAGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812121.1
CDS	245..6010
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956632.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956632.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACAAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGTAGAGGAC CAAACTCTTA CCCAGGATCT ACTGGAGCAG CTGTTGGAGC CCATGACGCC 
TGTCGAGATG CAAAAGAGAA GCGCTCTAAA AGCCAAGATG CAGATTCACC TGGAAGTTCT 
GGGGCCCCTG AAAACTTGAC ATTCAAGGAA CGCCAGCGTC TTTTTTCACA AGGTCAAGAT 
GTATCCAATA AAGTGAAAGC TTCTCGTAAA TTAACAGAAC TGGAGAATGA ACTGAACACA 
AAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956633.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812122.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

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CloneID	OPc87345
Clone ID Related Accession (Same CDS sequence)	XM_016956633.1
Accession Version	XM_016956633.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812122.1
CDS	245..6007
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956633.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956633.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956634.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812123.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

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CloneID	OPc87346
Clone ID Related Accession (Same CDS sequence)	XM_016956634.1
Accession Version	XM_016956634.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812123.1
CDS	245..6007
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956634.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956634.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956635.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812124.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

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CloneID	OPc87347
Clone ID Related Accession (Same CDS sequence)	XM_016956635.1
Accession Version	XM_016956635.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5763bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812124.1
CDS	245..6007
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956635.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956635.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956636.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812125.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

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CloneID	OPc87348
Clone ID Related Accession (Same CDS sequence)	XM_016956636.1
Accession Version	XM_016956636.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACAAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812125.1
CDS	245..6004
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956636.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956636.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGTCA GGATAAGTAC TCCAGCACAA GAAAGTCTCA TGGGGACCTT 
CTTCCTGCCC CTCTTAAACC ACGGCCACCC CCCTGCCAGC CACGCCCGGC CTCCGATGGC 
ATCTTTCTCT CCAACTCATT CCAGCCACCC TCAGCCAAAG CCAACAGTAC TGCTCACAAA 
AAAGGCCAGC CCCCGCCCCC TCCAAAAAAA TCCAGCTCCT ACCATCCCAG CCACTGCAAA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956637.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812126.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

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CloneID	OPc87349
Clone ID Related Accession (Same CDS sequence)	XM_016956637.1
Accession Version	XM_016956637.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812126.1
CDS	245..6004
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956637.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956637.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956638.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812127.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

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CloneID	OPc87350
Clone ID Related Accession (Same CDS sequence)	XM_016956638.1
Accession Version	XM_016956638.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5760bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGTTT CTGGGCCTGA ACTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGCGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT 
GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAAAGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC 
TGGGGTGCAA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG 
CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCACTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC 
CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT 
TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812127.1
CDS	245..6004
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956638.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956638.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGGGAGAA GGAATCACTT TGCCTACTAC 
AACTATCACA CTTACGAAGA TGGTTCTGAC TCTAGAGATA AGCCAAAGCT TTACCGCCTT 
CAGTTAAGTG TTACTGAAGT TGGGACAGAA AAGTTGGATG ACAACTCTAT CCAGTTGTTT 
GGCCCAGGAA TTCAGCCCCA TCACTGTGAC CTTACCAACA TGGATGGAGT GGTCACTGTG 
ACGCCCAGAA GTATGGACGC AGAAACCTAC GTGGAAGGCC AGCGCATCTC AGAAACCACC 
ATGCTGCAGA GTGGCATGAA AGTGCAGTTT GGGGCGTCCC ATGTATTTAA GTTTGTGGAC 
CCCAGTCAGG ATCATGCTCT TGCAAAAAGA TCTGTGGATG GAGGCCTGAT GGTTAAGGGC 
CCAAGACATA AACCTGGAAT TGTTCAGGAG ACAACTTTTG ATTTGGGAGG AGATATTCAT 
AGTGGGACAG CATTACCGAC AAGCAAGAGC ACCACTAGGC TGGACAGCGA CAGAGTGTCG 
TCTGCCTCTA GCACAGCCGA GCGGGGAATG GTGAAGCCGA TGATCAGAGT AGAACAGCAG 
CCAGATTATC GCAGGCAAGA AAGCAGAACA CAGGATGTTT CTGGGCCTGA ACTGATACTA 
CCTGCAAGCA TTGAATTCAG GGAAAGTTCT GAAGATTCAT TTTTGTCTGC CATTATAAAT 
TATACTAATA GCTCTACAGT CCACTTTAAG TTGTCCCCTA CATATGTATT ATATATGGCA 
TGCCGGTATG TATTGTCCAA CCAGTACAGA CCTGACATCA GCCCTACAGA GCGCACACAT 
AAAGTCATTG CAGTCGTCAA CAAGATGGTG AGCATGATGG AGGGCGTCAT CCAGGAAGTA 
GACCAGGTTG ACCAGAAACA GAAGAATATT GCAGGGGCAC TTGCCTTCTG GATGGCAAAT 
GCATCTGAAC TTCTCAACTT CATTAAGCAA GACCGAGACC TTAGTCGGAT CACACTGGAT 
GCTCAAGATG TTTTAGCACA TTTGGTTCAA ATGGCATTTA AATACTTGGT TCACTGTCTT 
CAATCAGAAC TTAATAATTA CATGCCAGCC TTTCTAGATG ACCCTGAAGA GAACAGTCTG 
CAAAGACCAA AAATAGATGA TGTGCTGCAC ACGCTCACAG GAGCCATGTC CTTGCTACGA 
CGCTGCAGAG TCAATGCCGC CCTGACCATC CAGCTCTTCT CTCAGCTCTT CCACTTCATC 
AATATGTGGC TGTTCAATAG ATTGGTGACC GACCCAGATT CGGGGCTGTG CTCCCATTAC 
TGGGGTGCAA TTATCCGTCA GCAGTTGGGC CATATTGAAG CCTGGGCTGA GAAGCAGGGG 
CTGGAACTGG CTGCGGACTG TCATCTGAGC AGGATCGTGC AGGCAACGAC TTTGCTTACC 
ATGGATAAGT ATGCACCTGA TGACATTCCA AATATAAACA GCACCTGCTT TAAGTTAAAT 
TCACTACAAC TTCAAGCCTT ATTACAGAAC TATCACTGTG CACCTGATGA GCCTTTTATC 
CCAACGGATC TTATAGAAAA TGTAGTGACT GTGGCTGAAA ACACTGCCGA TGAGCTGGCC 
CGCAGTGATG GAAGGGAAGT GCAGTTGGAG GAGGATCCTG ATCTGCAGCT GCCGTTTCTT 
TTGCCAGAAG ATGGTTATTC TTGTGATGTT GTCAGAAACA TTCCAAATGG TTTACAAGAA 
TTTTTAGACC CTCTGTGCCA GAGAGGATTT TGCAGGTTAA TTCCTCACAC ACGTTCACCA 
GGTACTTGGA CAATATATTT TGAAGGTGCA GATTATGAAA GTCACCTTCT GCGTGAGAAC 
ACAGAGCTGG CTCAGCCTCT GAGGAAAGAA CCTGAAATAA TCACTGTGAC CCTAAAAAAG 
CAGAATGGAA TGGGCCTTAG CATTGTTGCA GCAAAGGGTG CTGGTCAAGA TAAACTAGGA 
ATCTATGTGA AGTCGGTTGT GAAAGGAGGT GCTGCAGATG TGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956639.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812128.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

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CloneID	OPc87351
Clone ID Related Accession (Same CDS sequence)	XM_016956639.1
Accession Version	XM_016956639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5757bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AACCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAGTCTC ATGGGGACCT TCTTCCTGCC 
CCTCTTAAAC CACGGCCACC CCCCTGCCAG CCACGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCAGCCAAA GCCAACAGTA CTGCTCACAA AAAAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ATCCAGCTCC TACCATCCCA GCCACTGCAA AGGTAGAGGA 
CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGAGAT 
GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT 
GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT 
AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812128.1
CDS	245..6001
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARTAMPAISVLDLDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956639.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956639.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGG ACGAGGAGCG GAGGCGGCAG CAGCAGTTAG AAGAGATGCG CAAGCGGGAA 
GCGGAAGACC GAGCGAGGCA AGAGGAAGAG CGCCGGCGGC AGGAGGAGGA GCGAACAAAA 
CGAGACGCTG AAGAAAAGAG GCGACAGGAA GAAGGGTATT ACAGCCGCCT GGAAGCCGAG 
AGGCGCAGAC AGCACGACGA GGCGGCGCGC AGGTTGCTGG AACCCGAGGC GCCCGGTCTG 
TGCCGCCCTC CGCTTCCCCG GGACTACGAG CCCCCGTCCC CGTCCCCCGC GCCCGGCGCC 
CCTCCTCCCC CGCCTCAGCG AAACGCCTCC TACCTCAAAA CACAGGTCCT CTCCCCCGAC 
TCGCTGTTCA CTGCCAAGTT TGTTGCATAC AATGAGGAGG AGGAGGAGGA GGACTGCAGC 
CTAGCAGGTC AGGATAAGTA CTCCAGCACA AGAAAGTCTC ATGGGGACCT TCTTCCTGCC 
CCTCTTAAAC CACGGCCACC CCCCTGCCAG CCACGCCCGG CCTCCGATGG CATCTTTCTC 
TCCAACTCAT TCCAGCCACC CTCAGCCAAA GCCAACAGTA CTGCTCACAA AAAAGGCCAG 
CCCCCGCCCC CTCCAAAAAA ATCCAGCTCC TACCATCCCA GCCACTGCAA AGGTAGAGGA 
CCAAACTCTT ACCCAGGATC TACTGGAGCA GCTGTTGGAG CCCATGACGC CTGTCGAGAT 
GCAAAAGAGA AGCGCTCTAA AAGCCAAGAT GCAGATTCAC CTGGAAGTTC TGGGGCCCCT 
GAAAACTTGA CATTCAAGGA ACGCCAGCGT CTTTTTTCAC AAGGTCAAGA TGTATCCAAT 
AAAGTGAAAG CTTCTCGTAA ATTAACAGAA CTGGAGAATG AACTGAACAC AAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956640.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812129.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

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CloneID	OPc87352
Clone ID Related Accession (Same CDS sequence)	XM_016956640.1
Accession Version	XM_016956640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5745bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAA CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGTCAG 
GATAAGTACT CCAGCACAAG AAAGTCTCAT GGGGACCTTC TTCCTGCCCC TCTTAAACCA 
CGGCCACCCC CCTGCCAGCC ACGCCCGGCC TCCGATGGCA TCTTTCTCTC CAACTCATTC 
CAGCCACCCT CAGCCAAAGC CAACAGTACT GCTCACAAAA AAGGCCAGCC CCCGCCCCCT 
CCAAAAAAAT CCAGCTCCTA CCATCCCAGC CACTGCAAAG GTAGAGGACC AAACTCTTAC 
CCAGGATCTA CTGGAGCAGC TGTTGGAGCC CATGACGCCT GTCGAGATGC AAAAGAGAAG 
CGCTCTAAAA GCCAAGATGC AGATTCACCT GGAAGTTCTG GGGCCCCTGA AAACTTGACA 
TTCAAGGAAC GCCAGCGTCT TTTTTCACAA GGTCAAGATG TATCCAATAA AGTGAAAGCT 
TCTCGTAAAT TAACAGAACT GGAGAATGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812129.1
CDS	245..5989
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956640.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956640.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGCAGAC TGCTATGCCA GCAATCTCTG TTCTAGATTT GTTGCAGGAC 
GAGGAGCGGA GGCGGCAGCA GCAGTTAGAA GAGATGCGCA AGCGGGAAGC GGAAGACCGA 
GCGAGGCAAG AGGAAGAGCG CCGGCGGCAG GAGGAGGAGC GAACAAAACG AGACGCTGAA 
GAAAAGAGGC GACAGGAAGA AGGGTATTAC AGCCGCCTGG AAGCCGAGAG GCGCAGACAG 
CACGACGAGG CGGCGCGCAG GTTGCTGGAA CCCGAGGCGC CCGGTCTGTG CCGCCCTCCG 
CTTCCCCGGG ACTACGAGCC CCCGTCCCCG TCCCCCGCGC CCGGCGCCCC TCCTCCCCCG 
CCTCAGCGAA ACGCCTCCTA CCTCAAAACA CAGGTCCTCT CCCCCGACTC GCTGTTCACT 
GCCAAGTTTG TTGCATACAA TGAGGAGGAG GAGGAGGAGG ACTGCAGCCT AGCAGGTCAG 
GATAAGTACT CCAGCACAAG AAAGTCTCAT GGGGACCTTC TTCCTGCCCC TCTTAAACCA 
CGGCCACCCC CCTGCCAGCC ACGCCCGGCC TCCGATGGCA TCTTTCTCTC CAACTCATTC 
CAGCCACCCT CAGCCAAAGC CAACAGTACT GCTCACAAAA AAGGCCAGCC CCCGCCCCCT 
CCAAAAAAAT CCAGCTCCTA CCATCCCAGC CACTGCAAAG GTAGAGGACC AAACTCTTAC 
CCAGGATCTA CTGGAGCAGC TGTTGGAGCC CATGACGCCT GTCGAGATGC AAAAGAGAAG 
CGCTCTAAAA GCCAAGATGC AGATTCACCT GGAAGTTCTG GGGCCCCTGA AAACTTGACA 
TTCAAGGAAC GCCAGCGTCT TTTTTCACAA GGTCAAGATG TATCCAATAA AGTGAAAGCT 
TCTCGTAAAT TAACAGAACT GGAGAATGAA CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956641.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812130.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.

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CloneID	OPc87353
Clone ID Related Accession (Same CDS sequence)	XM_016956641.1
Accession Version	XM_016956641.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5742bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG 
AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GACGAGGCGG CGCGCAGGTT GCTGGAACCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT 
CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT 
CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC 
AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGTCAGGAT 
AAGTACTCCA GCACAAGAAA GTCTCATGGG GACCTTCTTC CTGCCCCTCT TAAACCACGG 
CCACCCCCCT GCCAGCCACG CCCGGCCTCC GATGGCATCT TTCTCTCCAA CTCATTCCAG 
CCACCCTCAG CCAAAGCCAA CAGTACTGCT CACAAAAAAG GCCAGCCCCC GCCCCCTCCA 
AAAAAATCCA GCTCCTACCA TCCCAGCCAC TGCAAAGGTA GAGGACCAAA CTCTTACCCA 
GGATCTACTG GAGCAGCTGT TGGAGCCCAT GACGCCTGTC GAGATGCAAA AGAGAAGCGC 
TCTAAAAGCC AAGATGCAGA TTCACCTGGA AGTTCTGGGG CCCCTGAAAA CTTGACATTC 
AAGGAACGCC AGCGTCTTTT TTCACAAGGT CAAGATGTAT CCAATAAAGT GAAAGCTTCT 
CGTAAATTAA CAGAACTGGA GAATGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812130.1
CDS	245..5986
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956641.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956641.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGCAGACTGC TATGCCAGCA ATCTCTGTTC TAGATTTGTT GCAGGACGAG 
GAGCGGAGGC GGCAGCAGCA GTTAGAAGAG ATGCGCAAGC GGGAAGCGGA AGACCGAGCG 
AGGCAAGAGG AAGAGCGCCG GCGGCAGGAG GAGGAGCGAA CAAAACGAGA CGCTGAAGAA 
AAGAGGCGAC AGGAAGAAGG GTATTACAGC CGCCTGGAAG CCGAGAGGCG CAGACAGCAC 
GACGAGGCGG CGCGCAGGTT GCTGGAACCC GAGGCGCCCG GTCTGTGCCG CCCTCCGCTT 
CCCCGGGACT ACGAGCCCCC GTCCCCGTCC CCCGCGCCCG GCGCCCCTCC TCCCCCGCCT 
CAGCGAAACG CCTCCTACCT CAAAACACAG GTCCTCTCCC CCGACTCGCT GTTCACTGCC 
AAGTTTGTTG CATACAATGA GGAGGAGGAG GAGGAGGACT GCAGCCTAGC AGGTCAGGAT 
AAGTACTCCA GCACAAGAAA GTCTCATGGG GACCTTCTTC CTGCCCCTCT TAAACCACGG 
CCACCCCCCT GCCAGCCACG CCCGGCCTCC GATGGCATCT TTCTCTCCAA CTCATTCCAG 
CCACCCTCAG CCAAAGCCAA CAGTACTGCT CACAAAAAAG GCCAGCCCCC GCCCCCTCCA 
AAAAAATCCA GCTCCTACCA TCCCAGCCAC TGCAAAGGTA GAGGACCAAA CTCTTACCCA 
GGATCTACTG GAGCAGCTGT TGGAGCCCAT GACGCCTGTC GAGATGCAAA AGAGAAGCGC 
TCTAAAAGCC AAGATGCAGA TTCACCTGGA AGTTCTGGGG CCCCTGAAAA CTTGACATTC 
AAGGAACGCC AGCGTCTTTT TTCACAAGGT CAAGATGTAT CCAATAAAGT GAAAGCTTCT 
CGTAAATTAA CAGAACTGGA GAATGAACTG AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956642.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812131.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.

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CloneID	OPc87354
Clone ID Related Accession (Same CDS sequence)	XM_016956642.1
Accession Version	XM_016956642.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5730bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC 
GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC 
TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA 
TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GTCAGGATAA GTACTCCAGC 
ACAAGAAAGT CTCATGGGGA CCTTCTTCCT GCCCCTCTTA AACCACGGCC ACCCCCCTGC 
CAGCCACGCC CGGCCTCCGA TGGCATCTTT CTCTCCAACT CATTCCAGCC ACCCTCAGCC 
AAAGCCAACA GTACTGCTCA CAAAAAAGGC CAGCCCCCGC CCCCTCCAAA AAAATCCAGC 
TCCTACCATC CCAGCCACTG CAAAGGTAGA GGACCAAACT CTTACCCAGG ATCTACTGGA 
GCAGCTGTTG GAGCCCATGA CGCCTGTCGA GATGCAAAAG AGAAGCGCTC TAAAAGCCAA 
GATGCAGATT CACCTGGAAG TTCTGGGGCC CCTGAAAACT TGACATTCAA GGAACGCCAG 
CGTCTTTTTT CACAAGGTCA AGATGTATCC AATAAAGTGA AAGCTTCTCG TAAATTAACA 
GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812131.1
CDS	244..5973
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956642.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956642.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGTTGC AGGACGAGGA GCGGAGGCGG 
CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA 
GAGCGCCGGC GGCAGGAGGA GGAGCGAACA AAACGAGACG CTGAAGAAAA GAGGCGACAG 
GAAGAAGGGT ATTACAGCCG CCTGGAAGCC GAGAGGCGCA GACAGCACGA CGAGGCGGCG 
CGCAGGTTGC TGGAACCCGA GGCGCCCGGT CTGTGCCGCC CTCCGCTTCC CCGGGACTAC 
GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC GCCCCTCCTC CCCCGCCTCA GCGAAACGCC 
TCCTACCTCA AAACACAGGT CCTCTCCCCC GACTCGCTGT TCACTGCCAA GTTTGTTGCA 
TACAATGAGG AGGAGGAGGA GGAGGACTGC AGCCTAGCAG GTCAGGATAA GTACTCCAGC 
ACAAGAAAGT CTCATGGGGA CCTTCTTCCT GCCCCTCTTA AACCACGGCC ACCCCCCTGC 
CAGCCACGCC CGGCCTCCGA TGGCATCTTT CTCTCCAACT CATTCCAGCC ACCCTCAGCC 
AAAGCCAACA GTACTGCTCA CAAAAAAGGC CAGCCCCCGC CCCCTCCAAA AAAATCCAGC 
TCCTACCATC CCAGCCACTG CAAAGGTAGA GGACCAAACT CTTACCCAGG ATCTACTGGA 
GCAGCTGTTG GAGCCCATGA CGCCTGTCGA GATGCAAAAG AGAAGCGCTC TAAAAGCCAA 
GATGCAGATT CACCTGGAAG TTCTGGGGCC CCTGAAAACT TGACATTCAA GGAACGCCAG 
CGTCTTTTTT CACAAGGTCA AGATGTATCC AATAAAGTGA AAGCTTCTCG TAAATTAACA 
GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956643.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812132.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

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CloneID	OPc87355
Clone ID Related Accession (Same CDS sequence)	XM_016956643.1
Accession Version	XM_016956643.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5724bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGTCAGG ATAAGTACTC CAGCACAAGA 
AAGTCTCATG GGGACCTTCT TCCTGCCCCT CTTAAACCAC GGCCACCCCC CTGCCAGCCA 
CGCCCGGCCT CCGATGGCAT CTTTCTCTCC AACTCATTCC AGCCACCCTC AGCCAAAGCC 
AACAGTACTG CTCACAAAAA AGGCCAGCCC CCGCCCCCTC CAAAAAAATC CAGCTCCTAC 
CATCCCAGCC ACTGCAAAGG TAGAGGACCA AACTCTTACC CAGGATCTAC TGGAGCAGCT 
GTTGGAGCCC ATGACGCCTG TCGAGATGCA AAAGAGAAGC GCTCTAAAAG CCAAGATGCA 
GATTCACCTG GAAGTTCTGG GGCCCCTGAA AACTTGACAT TCAAGGAACG CCAGCGTCTT 
TTTTCACAAG GTCAAGATGT ATCCAATAAA GTGAAAGCTT CTCGTAAATT AACAGAACTG 
GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812132.1
CDS	245..5968
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956643.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956643.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGTCAGG ATAAGTACTC CAGCACAAGA 
AAGTCTCATG GGGACCTTCT TCCTGCCCCT CTTAAACCAC GGCCACCCCC CTGCCAGCCA 
CGCCCGGCCT CCGATGGCAT CTTTCTCTCC AACTCATTCC AGCCACCCTC AGCCAAAGCC 
AACAGTACTG CTCACAAAAA AGGCCAGCCC CCGCCCCCTC CAAAAAAATC CAGCTCCTAC 
CATCCCAGCC ACTGCAAAGG TAGAGGACCA AACTCTTACC CAGGATCTAC TGGAGCAGCT 
GTTGGAGCCC ATGACGCCTG TCGAGATGCA AAAGAGAAGC GCTCTAAAAG CCAAGATGCA 
GATTCACCTG GAAGTTCTGG GGCCCCTGAA AACTTGACAT TCAAGGAACG CCAGCGTCTT 
TTTTCACAAG GTCAAGATGT ATCCAATAAA GTGAAAGCTT CTCGTAAATT AACAGAACTG 
GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956644.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812133.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

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CloneID	OPc87356
Clone ID Related Accession (Same CDS sequence)	XM_016956644.1
Accession Version	XM_016956644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGTT 
TCTGGGCCTG AACTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGCGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCA ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCACTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCGCAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAAGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCTAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCATCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCAC CAACCAGATA GGGCTGCCGT CTGCACAGGT GGCTGCTGCC 
GAACGGAAAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATCACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGAGGACGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGACGAGG AGCGGAGGCG GCAGCAGCAG 
TTAGAAGAGA TGCGCAAGCG GGAAGCGGAA GACCGAGCGA GGCAAGAGGA AGAGCGCCGG 
CGGCAGGAGG AGGAGCGAAC AAAACGAGAC GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG ACGAGGCGGC GCGCAGGTTG 
CTGGAACCCG AGGCGCCCGG TCTGTGCCGC CCTCCGCTTC CCCGGGACTA CGAGCCCCCG 
TCCCCGTCCC CCGCGCCCGG CGCCCCTCCT CCCCCGCCTC AGCGAAACGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC CGACTCGCTG TTCACTGCCA AGTTTGTTGC ATACAATGAG 
GAGGAGGAGG AGGAGGACTG CAGCCTAGCA GGTCAGGATA AGTACTCCAG CACAAGAAAG 
TCTCATGGGG ACCTTCTTCC TGCCCCTCTT AAACCACGGC CACCCCCCTG CCAGCCACGC 
CCGGCCTCCG ATGGCATCTT TCTCTCCAAC TCATTCCAGC CACCCTCAGC CAAAGCCAAC 
AGTACTGCTC ACAAAAAAGG CCAGCCCCCG CCCCCTCCAA AAAAATCCAG CTCCTACCAT 
CCCAGCCACT GCAAAGGTAG AGGACCAAAC TCTTACCCAG GATCTACTGG AGCAGCTGTT 
GGAGCCCATG ACGCCTGTCG AGATGCAAAA GAGAAGCGCT CTAAAAGCCA AGATGCAGAT 
TCACCTGGAA GTTCTGGGGC CCCTGAAAAC TTGACATTCA AGGAACGCCA GCGTCTTTTT 
TCACAAGGTC AAGATGTATC CAATAAAGTG AAAGCTTCTC GTAAATTAAC AGAACTGGAG 
AATGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812133.1
CDS	245..5965
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956644.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956644.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG ATGGTTCTGA CTCTAGAGAT 
AAGCCAAAGC TTTACCGCCT TCAGTTAAGT GTTACTGAAG TTGGGACAGA AAAGTTGGAT 
GACAACTCTA TCCAGTTGTT TGGCCCAGGA ATTCAGCCCC ATCACTGTGA CCTTACCAAC 
ATGGATGGAG TGGTCACTGT GACGCCCAGA AGTATGGACG CAGAAACCTA CGTGGAAGGC 
CAGCGCATCT CAGAAACCAC CATGCTGCAG AGTGGCATGA AAGTGCAGTT TGGGGCGTCC 
CATGTATTTA AGTTTGTGGA CCCCAGTCAG GATCATGCTC TTGCAAAAAG ATCTGTGGAT 
GGAGGCCTGA TGGTTAAGGG CCCAAGACAT AAACCTGGAA TTGTTCAGGA GACAACTTTT 
GATTTGGGAG GAGATATTCA TAGTGGGACA GCATTACCGA CAAGCAAGAG CACCACTAGG 
CTGGACAGCG ACAGAGTGTC GTCTGCCTCT AGCACAGCCG AGCGGGGAAT GGTGAAGCCG 
ATGATCAGAG TAGAACAGCA GCCAGATTAT CGCAGGCAAG AAAGCAGAAC ACAGGATGTT 
TCTGGGCCTG AACTGATACT ACCTGCAAGC ATTGAATTCA GGGAAAGTTC TGAAGATTCA 
TTTTTGTCTG CCATTATAAA TTATACTAAT AGCTCTACAG TCCACTTTAA GTTGTCCCCT 
ACATATGTAT TATATATGGC ATGCCGGTAT GTATTGTCCA ACCAGTACAG ACCTGACATC 
AGCCCTACAG AGCGCACACA TAAAGTCATT GCAGTCGTCA ACAAGATGGT GAGCATGATG 
GAGGGCGTCA TCCAGGAAGT AGACCAGGTT GACCAGAAAC AGAAGAATAT TGCAGGGGCA 
CTTGCCTTCT GGATGGCAAA TGCATCTGAA CTTCTCAACT TCATTAAGCA AGACCGAGAC 
CTTAGTCGGA TCACACTGGA TGCTCAAGAT GTTTTAGCAC ATTTGGTTCA AATGGCATTT 
AAATACTTGG TTCACTGTCT TCAATCAGAA CTTAATAATT ACATGCCAGC CTTTCTAGAT 
GACCCTGAAG AGAACAGTCT GCAAAGACCA AAAATAGATG ATGTGCTGCA CACGCTCACA 
GGAGCCATGT CCTTGCTACG ACGCTGCAGA GTCAATGCCG CCCTGACCAT CCAGCTCTTC 
TCTCAGCTCT TCCACTTCAT CAATATGTGG CTGTTCAATA GATTGGTGAC CGACCCAGAT 
TCGGGGCTGT GCTCCCATTA CTGGGGTGCA ATTATCCGTC AGCAGTTGGG CCATATTGAA 
GCCTGGGCTG AGAAGCAGGG GCTGGAACTG GCTGCGGACT GTCATCTGAG CAGGATCGTG 
CAGGCAACGA CTTTGCTTAC CATGGATAAG TATGCACCTG ATGACATTCC AAATATAAAC 
AGCACCTGCT TTAAGTTAAA TTCACTACAA CTTCAAGCCT TATTACAGAA CTATCACTGT 
GCACCTGATG AGCCTTTTAT CCCAACGGAT CTTATAGAAA ATGTAGTGAC TGTGGCTGAA 
AACACTGCCG ATGAGCTGGC CCGCAGTGAT GGAAGGGAAG TGCAGTTGGA GGAGGATCCT 
GATCTGCAGC TGCCGTTTCT TTTGCCAGAA GATGGTTATT CTTGTGATGT TGTCAGAAAC 
ATTCCAAATG GTTTACAAGA ATTTTTAGAC CCTCTGTGCC AGAGAGGATT TTGCAGGTTA 
ATTCCTCACA CACGTTCACC AGGTACTTGG ACAATATATT TTGAAGGTGC AGATTATGAA 
AGTCACCTTC TGCGTGAGAA CACAGAGCTG GCTCAGCCTC TGAGGAAAGA ACCTGAAATA 
ATCACTGTGA CCCTAAAAAA GCAGAATGGA ATGGGCCTTA GCATTGTTGC AGCAAAGGGT 
GCTGGTCAAG ATAAACTAGG AATCTATGTG AAGTCGGTTG TGAAAGGAGG TGCTGCAGAT 
GTGGATGGAC GTCTAGCTGC AGGTGATCAG CTCCTCAGTG TGGATGGACG AAGTCTGGTT 
GGACTCTCTC AGGAAAGGGC AGCAGAACTC ATGACAAGAA CAAGCTCTGT GGTGACACTG 
GAAGTAGCAA AGCAGGGTGC CATCTACCAC GGTCTGGCCA CCCTTCTCAA TCAGCCATCC 
CCCATGATGC AGAGAATTTC AGATCGTCGT GGCTCAGGTA AACCCCGACC AAAGAGTGAA 
GGCTTTGAGC TCTATAATAA TTCAACTCAA AATGGGTCTC CTGAGAGTCC TCAGCTGCCT 
TGGGCAGAAT ATAGTGAACC AAAGAAATTG CCTGGTGATG ACAGACTGAT GAAAAATAGA 
GCTGATCACC GTTCCAGCCC CAACGTAGCA AATCAGCCTC CTAGTCCTGG AGGGAAAAGT 
GCATATGCCT CTGGAACAAC AGCGAAGATA ACATCTGTCT CTACTGGAAA CCTCTGCACT 
GAGGAGCAGA CGCCTCCGCC TAGACCTGAA GCCTACCCCA TCCCCACTCA GACGTACACC 
AGAGAGTATT TTACCTTCCC AGCTTCCAAA TCCCAGGATC GGATGGCTCC TCCTCAGAAC 
CAGTGGCCAA ATTATGAGGA AAAGCCGCAT ATGCACACAG ATAGTAATCA TTCCAGTATT 
GCAATTCAGC GTGTTACACG TTCCCAAGAA GAACTTCGAG AAGATAAAGC TTACCAACTT 
GAGCGGCATC GAATAGAGGC AGCTATGGAC CGAAAGTCTG ATAGTGATAT GTGGATAAAT 
CAGAGCTCCT CACTGGACTC CAGTACCTCT AGCCAAGAGC ATCTGAACCA TTCCTCTAAG 
TCGGTCACCC CTGCTTCTAC ACTGACCAAA AGTGGCCCTG GCCGTTGGAA AACACCAGCA 
GCCATACCGG CCACCCCTGT GGCCATCTCC CAGCCAATCC GAACAGACCT GCCTCCGCCA 
CCCCCGCCAC CTCCAGTCCA CTATGCCGGT GATTTCGATG GAATGTCCAT GGATTTGCCT 
CTCCCACCAC CCCCTTCCAC CAACCAGATA GGGCTGCCGT CTGCACAGGT GGCTGCTGCC 
GAACGGAAAA AGAGAGAAGA ACATCAGCGT TGGTATGAGA AGGAGAAGGC CCGCCTGGAG 
GAGGAGCGGG AGAGGAAGCG GAGAGAGCAG GAGAGGAAGT TGGGCCAGAT GCGCACTCAG 
TCCTTAAACC CTGCTCCGTT TTCTCCCCTG ACTGCACAGC AGATGAAGCC CGAAAAGCCT 
TCCACACTCC AGCGGCCACA GGAAACAGTC ATTCGGGAGC TGCAGCCTCA GCAGCAGCCC 
CGCACGATCG AGCGCAGAGA CTTGCAGTAC ATCACAGTCA GCAAAGAGGA GCTTTCCTCG 
GGGGACAGTC TGTCCCCCGA CCCGTGGAAG CGGGACGCCA AGGAGAAGCT GGAGAAGCAG 
CAGCAGATGC ACATCGTGGA CATGCTGAGC AAGGAGATCC AGGAGCTCCA GAGCAAACCG 
GACCGCAGCG CCGAGGAGAG CGACCGGCTG CGCAAGCTCA TGCTGGAGTG GCAGTTCCAG 
AAGAGACTCC AGGAGTCGAA GCAGAAGGAC GAAGATGACG AGGAGGAGGA GGAGGACGAT 
GTGGACACCA TGCTGATCAT GCAGCGCCTG GAGGCTGAAC GAAGAGCGAG GCAGACTGCT 
ATGCCAGCAA TCTCTGTTCT AGATTTGTTG CAGGACGAGG AGCGGAGGCG GCAGCAGCAG 
TTAGAAGAGA TGCGCAAGCG GGAAGCGGAA GACCGAGCGA GGCAAGAGGA AGAGCGCCGG 
CGGCAGGAGG AGGAGCGAAC AAAACGAGAC GCTGAAGAAA AGAGGCGACA GGAAGAAGGG 
TATTACAGCC GCCTGGAAGC CGAGAGGCGC AGACAGCACG ACGAGGCGGC GCGCAGGTTG 
CTGGAACCCG AGGCGCCCGG TCTGTGCCGC CCTCCGCTTC CCCGGGACTA CGAGCCCCCG 
TCCCCGTCCC CCGCGCCCGG CGCCCCTCCT CCCCCGCCTC AGCGAAACGC CTCCTACCTC 
AAAACACAGG TCCTCTCCCC CGACTCGCTG TTCACTGCCA AGTTTGTTGC ATACAATGAG 
GAGGAGGAGG AGGAGGACTG CAGCCTAGCA GGTCAGGATA AGTACTCCAG CACAAGAAAG 
TCTCATGGGG ACCTTCTTCC TGCCCCTCTT AAACCACGGC CACCCCCCTG CCAGCCACGC 
CCGGCCTCCG ATGGCATCTT TCTCTCCAAC TCATTCCAGC CACCCTCAGC CAAAGCCAAC 
AGTACTGCTC ACAAAAAAGG CCAGCCCCCG CCCCCTCCAA AAAAATCCAG CTCCTACCAT 
CCCAGCCACT GCAAAGGTAG AGGACCAAAC TCTTACCCAG GATCTACTGG AGCAGCTGTT 
GGAGCCCATG ACGCCTGTCG AGATGCAAAA GAGAAGCGCT CTAAAAGCCA AGATGCAGAT 
TCACCTGGAA GTTCTGGGGC CCCTGAAAAC TTGACATTCA AGGAACGCCA GCGTCTTTTT 
TCACAAGGTC AAGATGTATC CAATAAAGTG AAAGCTTCTC GTAAATTAAC AGAACTGGAG 
AATGAACTGA ACACAAAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956645.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812134.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.

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CloneID	OPc87357
Clone ID Related Accession (Same CDS sequence)	XM_016956645.1
Accession Version	XM_016956645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812134.1
CDS	245..5944
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956645.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956645.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956646.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812135.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA.

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CloneID	OPc87358
Clone ID Related Accession (Same CDS sequence)	XM_016956646.1
Accession Version	XM_016956646.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812135.1
CDS	245..5944
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956646.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956646.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGATGGACG TCTAGCTGCA 
GGTGATCAGC TCCTCAGTGT GGATGGACGA AGTCTGGTTG GACTCTCTCA GGAAAGGGCA 
GCAGAACTCA TGACAAGAAC AAGCTCTGTG GTGACACTGG AAGTAGCAAA GCAGGGTGCC 
ATCTACCACG GTCTGGCCAC CCTTCTCAAT CAGCCATCCC CCATGATGCA GAGAATTTCA 
GATCGTCGTG GCTCAGGTAA ACCCCGACCA AAGAGTGAAG GCTTTGAGCT CTATAATAAT 
TCAACTCAAA ATGGGTCTCC TGAGAGTCCT CAGCTGCCTT GGGCAGAATA TAGTGAACCA 
AAGAAATTGC CTGGTGATGA CAGACTGATG AAAAATAGAG CTGATCACCG TTCCAGCCCC 
AACGTAGCAA ATCAGCCTCC TAGTCCTGGA GGGAAAAGTG CATATGCCTC TGGAACAACA 
GCGAAGATAA CATCTGTCTC TACTGGAAAC CTCTGCACTG AGGAGCAGAC GCCTCCGCCT 
AGACCTGAAG CCTACCCCAT CCCCACTCAG ACGTACACCA GAGAGTATTT TACCTTCCCA 
GCTTCCAAAT CCCAGGATCG GATGGCTCCT CCTCAGAACC AGTGGCCAAA TTATGAGGAA 
AAGCCGCATA TGCACACAGA TAGTAATCAT TCCAGTATTG CAATTCAGCG TGTTACACGT 
TCCCAAGAAG AACTTCGAGA AGATAAAGCT TACCAACTTG AGCGGCATCG AATAGAGGCA 
GCTATGGACC GAAAGTCTGA TAGTGATATG TGGATAAATC AGAGCTCCTC ACTGGACTCC 
AGTACCTCTA GCCAAGAGCA TCTGAACCAT TCCTCTAAGT CGGTCACCCC TGCTTCTACA 
CTGACCAAAA GTGGCCCTGG CCGTTGGAAA ACACCAGCAG CCATACCGGC CACCCCTGTG 
GCCATCTCCC AGCCAATCCG AACAGACCTG CCTCCGCCAC CCCCGCCACC TCCAGTCCAC 
TATGCCGGTG ATTTCGATGG AATGTCCATG GATTTGCCTC TCCCACCACC CCCTTCCACC 
AACCAGATAG GGCTGCCGTC TGCACAGGTG GCTGCTGCCG AACGGAAAAA GAGAGAAGAA 
CATCAGCGTT GGTATGAGAA GGAGAAGGCC CGCCTGGAGG AGGAGCGGGA GAGGAAGCGG 
AGAGAGCAGG AGAGGAAGTT GGGCCAGATG CGCACTCAGT CCTTAAACCC TGCTCCGTTT 
TCTCCCCTGA CTGCACAGCA GATGAAGCCC GAAAAGCCTT CCACACTCCA GCGGCCACAG 
GAAACAGTCA TTCGGGAGCT GCAGCCTCAG CAGCAGCCCC GCACGATCGA GCGCAGAGAC 
TTGCAGTACA TCACAGTCAG CAAAGAGGAG CTTTCCTCGG GGGACAGTCT GTCCCCCGAC 
CCGTGGAAGC GGGACGCCAA GGAGAAGCTG GAGAAGCAGC AGCAGATGCA CATCGTGGAC 
ATGCTGAGCA AGGAGATCCA GGAGCTCCAG AGCAAACCGG ACCGCAGCGC CGAGGAGAGC 
GACCGGCTGC GCAAGCTCAT GCTGGAGTGG CAGTTCCAGA AGAGACTCCA GGAGTCGAAG 
CAGAAGGACG AAGATGACGA GGAGGAGGAG GAGGACGATG TGGACACCAT GCTGATCATG 
CAGCGCCTGG AGGCTGAACG AAGAGCGAGG CAGACTGCTA TGCCAGCAAT CTCTGTTCTA 
GATTTGTTGC AGGACGAGGA GCGGAGGCGG CAGCAGCAGT TAGAAGAGAT GCGCAAGCGG 
GAAGCGGAAG ACCGAGCGAG GCAAGAGGAA GAGCGCCGGC GGCAGGAGGA GGAGCGAACA 
AAACGAGACG CTGAAGAAAA GAGGCGACAG GAAGAAGGGT ATTACAGCCG CCTGGAAGCC 
GAGAGGCGCA GACAGCACGA CGAGGCGGCG CGCAGGTTGC TGGAACCCGA GGCGCCCGGT 
CTGTGCCGCC CTCCGCTTCC CCGGGACTAC GAGCCCCCGT CCCCGTCCCC CGCGCCCGGC 
GCCCCTCCTC CCCCGCCTCA GCGAAACGCC TCCTACCTCA AAACACAGGT CCTCTCCCCC 
GACTCGCTGT TCACTGCCAA GTTTGTTGCA TACAATGAGG AGGAGGAGGA GGAGGACTGC 
AGCCTAGCAG GTCAGGATAA GTACTCCAGC ACAAGAAAGT CTCATGGGGA CCTTCTTCCT 
GCCCCTCTTA AACCACGGCC ACCCCCCTGC CAGCCACGCC CGGCCTCCGA TGGCATCTTT 
CTCTCCAACT CATTCCAGCC ACCCTCAGCC AAAGCCAACA GTACTGCTCA CAAAAAAGGC 
CAGCCCCCGC CCCCTCCAAA AAAATCCAGC TCCTACCATC CCAGCCACTG CAAAGGTAGA 
GGACCAAACT CTTACCCAGG ATCTACTGGA GCAGCTGTTG GAGCCCATGA CGCCTGTCGA 
GATGCAAAAG AGAAGCGCTC TAAAAGCCAA GATGCAGATT CACCTGGAAG TTCTGGGGCC 
CCTGAAAACT TGACATTCAA GGAACGCCAG CGTCTTTTTT CACAAGGTCA AGATGTATCC 
AATAAAGTGA AAGCTTCTCG TAAATTAACA GAACTGGAGA ATGAACTGAA CACAAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956647.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812136.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X16, mRNA.

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CloneID	OPc87359
Clone ID Related Accession (Same CDS sequence)	XM_016956647.1
Accession Version	XM_016956647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5694bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGAG GCGGCAGCAG CAGTTAGAAG AGATGCGCAA GCGGGAAGCG 
GAAGACCGAG CGAGGCAAGA GGAAGAGCGC CGGCGGCAGG AGGAGGAGCG AACAAAACGA 
GACGCTGAAG AAAAGAGGCG ACAGGAAGAA GGGTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGACGAGGC GGCGCGCAGG TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC 
CGCCCTCCGC TTCCCCGGGA CTACGAGCCC CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT 
CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC CTCAAAACAC AGGTCCTCTC CCCCGACTCG 
CTGTTCACTG CCAAGTTTGT TGCATACAAT GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA 
GCAGGTCAGG ATAAGTACTC CAGCACAAGA AAGTCTCATG GGGACCTTCT TCCTGCCCCT 
CTTAAACCAC GGCCACCCCC CTGCCAGCCA CGCCCGGCCT CCGATGGCAT CTTTCTCTCC 
AACTCATTCC AGCCACCCTC AGCCAAAGCC AACAGTACTG CTCACAAAAA AGGCCAGCCC 
CCGCCCCCTC CAAAAAAATC CAGCTCCTAC CATCCCAGCC ACTGCAAAGG TAGAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGAGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812136.1
CDS	245..5938
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956647.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956647.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGGCTCAA 
AGTAATGGAC CTGAAAAGCA GGAAAAAGAA GGGGTTATCC AGAACTTCAA GAGAACTCTC 
TCAAAGAAAG AAAAGAAGGA AAAAAAGAAG AGAGAAAAAG AGGCATTGCG ACAGGCATCT 
GATAAAGATG ATAGACCTTT CCAAGGGGAG GATGTTGAAA ATTCTCGACT GGCTGCTGAG 
GTTTACAAAG ACATGCCGGA AACCAGCTTT ACTCGAACCA TTTCTAATCC TGAGGTGGTT 
ATGAAACGAC GGAGGCAGCA AAAATTGGAA AAGAGAATGC AGGAATTTCG GAGCTCAGAT 
GGGCGGCCTG ATTCAGGTGG AACATTGAGA ATTTATGCAG ATAGTTTAAA ACCAAATATT 
CCCTACAAGA CAATCCTGCT GTCTACTACA GATCCTGCAG ACTTTGCTGT GGCTGAAGCT 
TTAGAGAAGT ATGGTCTGGA AAAAGAAAAC CCTAAGGATT ACTGCATCGC CCGGGTTATG 
CTTCCTCCTG GAGCCCAGCA TTCTGATGAA AAGGGTGCTA AAGAAATTAT TCTTGATGAT 
GATGAGTGTC CTTTACAAAT CTTCAGGGAA TGGCCAAGTG ACAAAGGGAT TTTAGTCTTT 
CAATTGAAGA GGAGGCCACC AGACCACATC CCAAAGAAAA CCAAGAAACA CTTGGAAGGC 
AAGACACCCA AGGGAAAGGA GAGAGCTGAC GGGTCTGGCT ATGGCTCCAC CCTTCCTCCG 
GAGAAGCTGC CCTATTTAGT AGAGTTAAGC CCAGATGGTT CTGACTCTAG AGATAAGCCA 
AAGCTTTACC GCCTTCAGTT AAGTGTTACT GAAGTTGGGA CAGAAAAGTT GGATGACAAC 
TCTATCCAGT TGTTTGGCCC AGGAATTCAG CCCCATCACT GTGACCTTAC CAACATGGAT 
GGAGTGGTCA CTGTGACGCC CAGAAGTATG GACGCAGAAA CCTACGTGGA AGGCCAGCGC 
ATCTCAGAAA CCACCATGCT GCAGAGTGGC ATGAAAGTGC AGTTTGGGGC GTCCCATGTA 
TTTAAGTTTG TGGACCCCAG TCAGGATCAT GCTCTTGCAA AAAGATCTGT GGATGGAGGC 
CTGATGGTTA AGGGCCCAAG ACATAAACCT GGAATTGTTC AGGAGACAAC TTTTGATTTG 
GGAGGAGATA TTCATAGTGG GACAGCATTA CCGACAAGCA AGAGCACCAC TAGGCTGGAC 
AGCGACAGAG TGTCGTCTGC CTCTAGCACA GCCGAGCGGG GAATGGTGAA GCCGATGATC 
AGAGTAGAAC AGCAGCCAGA TTATCGCAGG CAAGAAAGCA GAACACAGGA TGTTTCTGGG 
CCTGAACTGA TACTACCTGC AAGCATTGAA TTCAGGGAAA GTTCTGAAGA TTCATTTTTG 
TCTGCCATTA TAAATTATAC TAATAGCTCT ACAGTCCACT TTAAGTTGTC CCCTACATAT 
GTATTATATA TGGCATGCCG GTATGTATTG TCCAACCAGT ACAGACCTGA CATCAGCCCT 
ACAGAGCGCA CACATAAAGT CATTGCAGTC GTCAACAAGA TGGTGAGCAT GATGGAGGGC 
GTCATCCAGA AACAGAAGAA TATTGCAGGG GCACTTGCCT TCTGGATGGC AAATGCATCT 
GAACTTCTCA ACTTCATTAA GCAAGACCGA GACCTTAGTC GGATCACACT GGATGCTCAA 
GATGTTTTAG CACATTTGGT TCAAATGGCA TTTAAATACT TGGTTCACTG TCTTCAATCA 
GAACTTAATA ATTACATGCC AGCCTTTCTA GATGACCCTG AAGAGAACAG TCTGCAAAGA 
CCAAAAATAG ATGATGTGCT GCACACGCTC ACAGGAGCCA TGTCCTTGCT ACGACGCTGC 
AGAGTCAATG CCGCCCTGAC CATCCAGCTC TTCTCTCAGC TCTTCCACTT CATCAATATG 
TGGCTGTTCA ATAGATTGGT GACCGACCCA GATTCGGGGC TGTGCTCCCA TTACTGGGGT 
GCAATTATCC GTCAGCAGTT GGGCCATATT GAAGCCTGGG CTGAGAAGCA GGGGCTGGAA 
CTGGCTGCGG ACTGTCATCT GAGCAGGATC GTGCAGGCAA CGACTTTGCT TACCATGGAT 
AAGTATGCAC CTGATGACAT TCCAAATATA AACAGCACCT GCTTTAAGTT AAATTCACTA 
CAACTTCAAG CCTTATTACA GAACTATCAC TGTGCACCTG ATGAGCCTTT TATCCCAACG 
GATCTTATAG AAAATGTAGT GACTGTGGCT GAAAACACTG CCGATGAGCT GGCCCGCAGT 
GATGGAAGGG AAGTGCAGTT GGAGGAGGAT CCTGATCTGC AGCTGCCGTT TCTTTTGCCA 
GAAGATGGTT ATTCTTGTGA TGTTGTCAGA AACATTCCAA ATGGTTTACA AGAATTTTTA 
GACCCTCTGT GCCAGAGAGG ATTTTGCAGG TTAATTCCTC ACACACGTTC ACCAGGTACT 
TGGACAATAT ATTTTGAAGG TGCAGATTAT GAAAGTCACC TTCTGCGTGA GAACACAGAG 
CTGGCTCAGC CTCTGAGGAA AGAACCTGAA ATAATCACTG TGACCCTAAA AAAGCAGAAT 
GGAATGGGCC TTAGCATTGT TGCAGCAAAG GGTGCTGGTC AAGATAAACT AGGAATCTAT 
GTGAAGTCGG TTGTGAAAGG AGGTGCTGCA GATGTGGATG GACGTCTAGC TGCAGGTGAT 
CAGCTCCTCA GTGTGGATGG ACGAAGTCTG GTTGGACTCT CTCAGGAAAG GGCAGCAGAA 
CTCATGACAA GAACAAGCTC TGTGGTGACA CTGGAAGTAG CAAAGCAGGG TGCCATCTAC 
CACGGTCTGG CCACCCTTCT CAATCAGCCA TCCCCCATGA TGCAGAGAAT TTCAGATCGT 
CGTGGCTCAG GTAAACCCCG ACCAAAGAGT GAAGGCTTTG AGCTCTATAA TAATTCAACT 
CAAAATGGGT CTCCTGAGAG TCCTCAGCTG CCTTGGGCAG AATATAGTGA ACCAAAGAAA 
TTGCCTGGTG ATGACAGACT GATGAAAAAT AGAGCTGATC ACCGTTCCAG CCCCAACGTA 
GCAAATCAGC CTCCTAGTCC TGGAGGGAAA AGTGCATATG CCTCTGGAAC AACAGCGAAG 
ATAACATCTG TCTCTACTGG AAACCTCTGC ACTGAGGAGC AGACGCCTCC GCCTAGACCT 
GAAGCCTACC CCATCCCCAC TCAGACGTAC ACCAGAGAGT ATTTTACCTT CCCAGCTTCC 
AAATCCCAGG ATCGGATGGC TCCTCCTCAG AACCAGTGGC CAAATTATGA GGAAAAGCCG 
CATATGCACA CAGATAGTAA TCATTCCAGT ATTGCAATTC AGCGTGTTAC ACGTTCCCAA 
GAAGAACTTC GAGAAGATAA AGCTTACCAA CTTGAGCGGC ATCGAATAGA GGCAGCTATG 
GACCGAAAGT CTGATAGTGA TATGTGGATA AATCAGAGCT CCTCACTGGA CTCCAGTACC 
TCTAGCCAAG AGCATCTGAA CCATTCCTCT AAGTCGGTCA CCCCTGCTTC TACACTGACC 
AAAAGTGGCC CTGGCCGTTG GAAAACACCA GCAGCCATAC CGGCCACCCC TGTGGCCATC 
TCCCAGCCAA TCCGAACAGA CCTGCCTCCG CCACCCCCGC CACCTCCAGT CCACTATGCC 
GGTGATTTCG ATGGAATGTC CATGGATTTG CCTCTCCCAC CACCCCCTTC CACCAACCAG 
ATAGGGCTGC CGTCTGCACA GGTGGCTGCT GCCGAACGGA AAAAGAGAGA AGAACATCAG 
CGTTGGTATG AGAAGGAGAA GGCCCGCCTG GAGGAGGAGC GGGAGAGGAA GCGGAGAGAG 
CAGGAGAGGA AGTTGGGCCA GATGCGCACT CAGTCCTTAA ACCCTGCTCC GTTTTCTCCC 
CTGACTGCAC AGCAGATGAA GCCCGAAAAG CCTTCCACAC TCCAGCGGCC ACAGGAAACA 
GTCATTCGGG AGCTGCAGCC TCAGCAGCAG CCCCGCACGA TCGAGCGCAG AGACTTGCAG 
TACATCACAG TCAGCAAAGA GGAGCTTTCC TCGGGGGACA GTCTGTCCCC CGACCCGTGG 
AAGCGGGACG CCAAGGAGAA GCTGGAGAAG CAGCAGCAGA TGCACATCGT GGACATGCTG 
AGCAAGGAGA TCCAGGAGCT CCAGAGCAAA CCGGACCGCA GCGCCGAGGA GAGCGACCGG 
CTGCGCAAGC TCATGCTGGA GTGGCAGTTC CAGAAGAGAC TCCAGGAGTC GAAGCAGAAG 
GACGAAGATG ACGAGGAGGA GGAGGAGGAC GATGTGGACA CCATGCTGAT CATGCAGCGC 
CTGGAGGCTG AACGAAGAGC GAGGCAGACT GCTATGCCAG CAATCTCTGT TCTAGATTTG 
TTGCAGGACG AGGAGCGGAG GCGGCAGCAG CAGTTAGAAG AGATGCGCAA GCGGGAAGCG 
GAAGACCGAG CGAGGCAAGA GGAAGAGCGC CGGCGGCAGG AGGAGGAGCG AACAAAACGA 
GACGCTGAAG AAAAGAGGCG ACAGGAAGAA GGGTATTACA GCCGCCTGGA AGCCGAGAGG 
CGCAGACAGC ACGACGAGGC GGCGCGCAGG TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC 
CGCCCTCCGC TTCCCCGGGA CTACGAGCCC CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT 
CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC CTCAAAACAC AGGTCCTCTC CCCCGACTCG 
CTGTTCACTG CCAAGTTTGT TGCATACAAT GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA 
GCAGGTCAGG ATAAGTACTC CAGCACAAGA AAGTCTCATG GGGACCTTCT TCCTGCCCCT 
CTTAAACCAC GGCCACCCCC CTGCCAGCCA CGCCCGGCCT CCGATGGCAT CTTTCTCTCC 
AACTCATTCC AGCCACCCTC AGCCAAAGCC AACAGTACTG CTCACAAAAA AGGCCAGCCC 
CCGCCCCCTC CAAAAAAATC CAGCTCCTAC CATCCCAGCC ACTGCAAAGG TAGAGGACCA 
AACTCTTACC CAGGATCTAC TGGAGCAGCT GTTGGAGCCC ATGACGCCTG TCGAGATGCA 
AAAGAGAAGC GCTCTAAAAG CCAAGATGCA GATTCACCTG GAAGTTCTGG GGCCCCTGAA 
AACTTGACAT TCAAGGAACG CCAGCGTCTT TTTTCACAAG GTCAAGATGT ATCCAATAAA 
GTGAAAGCTT CTCGTAAATT AACAGAACTG GAGAATGAAC TGAACACAAA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956648.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812137.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

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CloneID	OPc87360
Clone ID Related Accession (Same CDS sequence)	XM_016956648.2 , XM_016956648.1
Accession Version	XM_016956648.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956648.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812137.1
CDS	258..5792
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956648.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956648.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956648.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812137.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA.

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CloneID	OPc87360
Clone ID Related Accession (Same CDS sequence)	XM_016956648.2 , XM_016956648.1
Accession Version	XM_016956648.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5535bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812137.1
CDS	244..5778
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956648.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956648.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGCAGA CTGCTATGCC AGCAATCTCT 
GTTCTAGATT TGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956649.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812138.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.

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CloneID	OPc87361
Clone ID Related Accession (Same CDS sequence)	XM_016956649.2 , XM_016956649.1
Accession Version	XM_016956649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956649.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT 
GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGAGATGCAA AAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812138.1
CDS	257..5749
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956649.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956649.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT 
GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGAGATGCAA AAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956649.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812138.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA.

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CloneID	OPc87361
Clone ID Related Accession (Same CDS sequence)	XM_016956649.2 , XM_016956649.1
Accession Version	XM_016956649.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5493bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT 
GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGAGATGCAA AAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812138.1
CDS	244..5736
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956649.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956649.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
GAAGTAGACC AGGTTGACCA GAAACAGAAG AATATTGCAG GGGCACTTGC CTTCTGGATG 
GCAAATGCAT CTGAACTTCT CAACTTCATT AAGCAAGACC GAGACCTTAG TCGGATCACA 
CTGGATGCTC AAGATGTTTT AGCACATTTG GTTCAAATGG CATTTAAATA CTTGGTTCAC 
TGTCTTCAAT CAGAACTTAA TAATTACATG CCAGCCTTTC TAGATGACCC TGAAGAGAAC 
AGTCTGCAAA GACCAAAAAT AGATGATGTG CTGCACACGC TCACAGGAGC CATGTCCTTG 
CTACGACGCT GCAGAGTCAA TGCCGCCCTG ACCATCCAGC TCTTCTCTCA GCTCTTCCAC 
TTCATCAATA TGTGGCTGTT CAATAGATTG GTGACCGACC CAGATTCGGG GCTGTGCTCC 
CATTACTGGG GTGCAATTAT CCGTCAGCAG TTGGGCCATA TTGAAGCCTG GGCTGAGAAG 
CAGGGGCTGG AACTGGCTGC GGACTGTCAT CTGAGCAGGA TCGTGCAGGC AACGACTTTG 
CTTACCATGG ATAAGTATGC ACCTGATGAC ATTCCAAATA TAAACAGCAC CTGCTTTAAG 
TTAAATTCAC TACAACTTCA AGCCTTATTA CAGAACTATC ACTGTGCACC TGATGAGCCT 
TTTATCCCAA CGGATCTTAT AGAAAATGTA GTGACTGTGG CTGAAAACAC TGCCGATGAG 
CTGGCCCGCA GTGATGGAAG GGAAGTGCAG TTGGAGGAGG ATCCTGATCT GCAGCTGCCG 
TTTCTTTTGC CAGAAGATGG TTATTCTTGT GATGTTGTCA GAAACATTCC AAATGGTTTA 
CAAGAATTTT TAGACCCTCT GTGCCAGAGA GGATTTTGCA GGTTAATTCC TCACACACGT 
TCACCAGGTA CTTGGACAAT ATATTTTGAA GGTGCAGATT ATGAAAGTCA CCTTCTGCGT 
GAGAACACAG AGCTGGCTCA GCCTCTGAGG AAAGAACCTG AAATAATCAC TGTGACCCTA 
AAAAAGCAGA ATGGAATGGG CCTTAGCATT GTTGCAGCAA AGGGTGCTGG TCAAGATAAA 
CTAGGAATCT ATGTGAAGTC GGTTGTGAAA GGAGGTGCTG CAGATGTGGA TGGACGTCTA 
GCTGCAGGTG ATCAGCTCCT CAGTGTGGAT GGACGAAGTC TGGTTGGACT CTCTCAGGAA 
AGGGCAGCAG AACTCATGAC AAGAACAAGC TCTGTGGTGA CACTGGAAGT AGCAAAGCAG 
GGTGCCATCT ACCACGGTCT GGCCACCCTT CTCAATCAGC CATCCCCCAT GATGCAGAGA 
ATTTCAGATC GTCGTGGCTC AGGTAAACCC CGACCAAAGA GTGAAGGCTT TGAGCTCTAT 
AATAATTCAA CTCAAAATGG GTCTCCTGAG AGTCCTCAGC TGCCTTGGGC AGAATATAGT 
GAACCAAAGA AATTGCCTGG TGATGACAGA CTGATGAAAA ATAGAGCTGA TCACCGTTCC 
AGCCCCAACG TAGCAAATCA GCCTCCTAGT CCTGGAGGGA AAAGTGCATA TGCCTCTGGA 
ACAACAGCGA AGATAACATC TGTCTCTACT GGAAACCTCT GCACTGAGGA GCAGACGCCT 
CCGCCTAGAC CTGAAGCCTA CCCCATCCCC ACTCAGACGT ACACCAGAGA GTATTTTACC 
TTCCCAGCTT CCAAATCCCA GGATCGGATG GCTCCTCCTC AGAACCAGTG GCCAAATTAT 
GAGGAAAAGC CGCATATGCA CACAGATAGT AATCATTCCA GTATTGCAAT TCAGCGTGTT 
ACACGTTCCC AAGAAGAACT TCGAGAAGAT AAAGCTTACC AACTTGAGCG GCATCGAATA 
GAGGCAGCTA TGGACCGAAA GTCTGATAGT GATATGTGGA TAAATCAGAG CTCCTCACTG 
GACTCCAGTA CCTCTAGCCA AGAGCATCTG AACCATTCCT CTAAGTCGGT CACCCCTGCT 
TCTACACTGA CCAAAAGTGG CCCTGGCCGT TGGAAAACAC CAGCAGCCAT ACCGGCCACC 
CCTGTGGCCA TCTCCCAGCC AATCCGAACA GACCTGCCTC CGCCACCCCC GCCACCTCCA 
GTCCACTATG CCGGTGATTT CGATGGAATG TCCATGGATT TGCCTCTCCC ACCACCCCCT 
TCCACCAACC AGATAGGGCT GCCGTCTGCA CAGGTGGCTG CTGCCGAACG GAAAAAGAGA 
GAAGAACATC AGCGTTGGTA TGAGAAGGAG AAGGCCCGCC TGGAGGAGGA GCGGGAGAGG 
AAGCGGAGAG AGCAGGAGAG GAAGTTGGGC CAGATGCGCA CTCAGTCCTT AAACCCTGCT 
CCGTTTTCTC CCCTGACTGC ACAGCAGATG AAGCCCGAAA AGCCTTCCAC ACTCCAGCGG 
CCACAGGAAA CAGTCATTCG GGAGCTGCAG CCTCAGCAGC AGCCCCGCAC GATCGAGCGC 
AGAGACTTGC AGTACATCAC AGTCAGCAAA GAGGAGCTTT CCTCGGGGGA CAGTCTGTCC 
CCCGACCCGT GGAAGCGGGA CGCCAAGGAG AAGCTGGAGA AGCAGCAGCA GATGCACATC 
GTGGACATGC TGAGCAAGGA GATCCAGGAG CTCCAGAGCA AACCGGACCG CAGCGCCGAG 
GAGAGCGACC GGCTGCGCAA GCTCATGCTG GAGTGGCAGT TCCAGAAGAG ACTCCAGGAG 
TCGAAGCAGA AGGACGAAGA TGACGAGGAG GAGGAGGAGG ACGATGTGGA CACCATGCTG 
ATCATGCAGC GCCTGGAGGC TGAACGAAGA GCGAGGGACG AGGAGCGGAG GCGGCAGCAG 
CAGTTAGAAG AGATGCGCAA GCGGGAAGCG GAAGACCGAG CGAGGCAAGA GGAAGAGCGC 
CGGCGGCAGG AGGAGGAGCG AACAAAACGA GACGCTGAAG AAAAGAGGCG ACAGGAAGAA 
GGGTATTACA GCCGCCTGGA AGCCGAGAGG CGCAGACAGC ACGACGAGGC GGCGCGCAGG 
TTGCTGGAAC CCGAGGCGCC CGGTCTGTGC CGCCCTCCGC TTCCCCGGGA CTACGAGCCC 
CCGTCCCCGT CCCCCGCGCC CGGCGCCCCT CCTCCCCCGC CTCAGCGAAA CGCCTCCTAC 
CTCAAAACAC AGGTCCTCTC CCCCGACTCG CTGTTCACTG CCAAGTTTGT TGCATACAAT 
GAGGAGGAGG AGGAGGAGGA CTGCAGCCTA GCAGGACCAA ACTCTTACCC AGGATCTACT 
GGAGCAGCTG TTGGAGCCCA TGACGCCTGT CGAGATGCAA AAGAGAAGCG CTCTAAAAGC 
CAAGATGCAG ATTCACCTGG AAGTTCTGGG GCCCCTGAAA ACTTGACATT CAAGGAACGC 
CAGCGTCTTT TTTCACAAGG TCAAGATGTA TCCAATAAAG TGAAAGCTTC TCGTAAATTA 
ACAGAACTGG AGAATGAACT GAACACAAAG TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956650.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812139.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X19, mRNA.

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CloneID	OPc87362
Clone ID Related Accession (Same CDS sequence)	XM_016956650.1 , XM_016956650.2
Accession Version	XM_016956650.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812139.1
CDS	243..5717
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956650.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956650.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956650.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812139.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X19, mRNA.

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CloneID	OPc87362
Clone ID Related Accession (Same CDS sequence)	XM_016956650.1 , XM_016956650.2
Accession Version	XM_016956650.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5475bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956650.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812139.1
CDS	256..5730
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956650.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956650.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAAGAAGAT TGGATATAGA TGAGAAACCT CTAGTTGTAC AACTGAATTG GAACAAAGAT 
GATCGGGAAG GCAGATTTGT TCTTAAGAAT GAGAATGACG CCATTCCTCC TAAGAAGGCT 
CAAAGTAATG GACCTGAAAA GCAGGAAAAA GAAGGGGTTA TCCAGAACTT CAAGAGAACT 
CTCTCAAAGA AAGAAAAGAA GGAAAAAAAG AAGAGAGAAA AAGAGGCATT GCGACAGGCA 
TCTGATAAAG ATGATAGACC TTTCCAAGGG GAGGATGTTG AAAATTCTCG ACTGGCTGCT 
GAGGTTTACA AAGACATGCC GGAAACCAGC TTTACTCGAA CCATTTCTAA TCCTGAGGTG 
GTTATGAAAC GACGGAGGCA GCAAAAATTG GAAAAGAGAA TGCAGGAATT TCGGAGCTCA 
GATGGGCGGC CTGATTCAGG TGGAACATTG AGAATTTATG CAGATAGTTT AAAACCAAAT 
ATTCCCTACA AGACAATCCT GCTGTCTACT ACAGATCCTG CAGACTTTGC TGTGGCTGAA 
GCTTTAGAGA AGTATGGTCT GGAAAAAGAA AACCCTAAGG ATTACTGCAT CGCCCGGGTT 
ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGAAA 
CAGAAGAATA TTGCAGGGGC ACTTGCCTTC TGGATGGCAA ATGCATCTGA ACTTCTCAAC 
TTCATTAAGC AAGACCGAGA CCTTAGTCGG ATCACACTGG ATGCTCAAGA TGTTTTAGCA 
CATTTGGTTC AAATGGCATT TAAATACTTG GTTCACTGTC TTCAATCAGA ACTTAATAAT 
TACATGCCAG CCTTTCTAGA TGACCCTGAA GAGAACAGTC TGCAAAGACC AAAAATAGAT 
GATGTGCTGC ACACGCTCAC AGGAGCCATG TCCTTGCTAC GACGCTGCAG AGTCAATGCC 
GCCCTGACCA TCCAGCTCTT CTCTCAGCTC TTCCACTTCA TCAATATGTG GCTGTTCAAT 
AGATTGGTGA CCGACCCAGA TTCGGGGCTG TGCTCCCATT ACTGGGGTGC AATTATCCGT 
CAGCAGTTGG GCCATATTGA AGCCTGGGCT GAGAAGCAGG GGCTGGAACT GGCTGCGGAC 
TGTCATCTGA GCAGGATCGT GCAGGCAACG ACTTTGCTTA CCATGGATAA GTATGCACCT 
GATGACATTC CAAATATAAA CAGCACCTGC TTTAAGTTAA ATTCACTACA ACTTCAAGCC 
TTATTACAGA ACTATCACTG TGCACCTGAT GAGCCTTTTA TCCCAACGGA TCTTATAGAA 
AATGTAGTGA CTGTGGCTGA AAACACTGCC GATGAGCTGG CCCGCAGTGA TGGAAGGGAA 
GTGCAGTTGG AGGAGGATCC TGATCTGCAG CTGCCGTTTC TTTTGCCAGA AGATGGTTAT 
TCTTGTGATG TTGTCAGAAA CATTCCAAAT GGTTTACAAG AATTTTTAGA CCCTCTGTGC 
CAGAGAGGAT TTTGCAGGTT AATTCCTCAC ACACGTTCAC CAGGTACTTG GACAATATAT 
TTTGAAGGTG CAGATTATGA AAGTCACCTT CTGCGTGAGA ACACAGAGCT GGCTCAGCCT 
CTGAGGAAAG AACCTGAAAT AATCACTGTG ACCCTAAAAA AGCAGAATGG AATGGGCCTT 
AGCATTGTTG CAGCAAAGGG TGCTGGTCAA GATAAACTAG GAATCTATGT GAAGTCGGTT 
GTGAAAGGAG GTGCTGCAGA TGTGGATGGA CGTCTAGCTG CAGGTGATCA GCTCCTCAGT 
GTGGATGGAC GAAGTCTGGT TGGACTCTCT CAGGAAAGGG CAGCAGAACT CATGACAAGA 
ACAAGCTCTG TGGTGACACT GGAAGTAGCA AAGCAGGGTG CCATCTACCA CGGTCTGGCC 
ACCCTTCTCA ATCAGCCATC CCCCATGATG CAGAGAATTT CAGATCGTCG TGGCTCAGGT 
AAACCCCGAC CAAAGAGTGA AGGCTTTGAG CTCTATAATA ATTCAACTCA AAATGGGTCT 
CCTGAGAGTC CTCAGCTGCC TTGGGCAGAA TATAGTGAAC CAAAGAAATT GCCTGGTGAT 
GACAGACTGA TGAAAAATAG AGCTGATCAC CGTTCCAGCC CCAACGTAGC AAATCAGCCT 
CCTAGTCCTG GAGGGAAAAG TGCATATGCC TCTGGAACAA CAGCGAAGAT AACATCTGTC 
TCTACTGGAA ACCTCTGCAC TGAGGAGCAG ACGCCTCCGC CTAGACCTGA AGCCTACCCC 
ATCCCCACTC AGACGTACAC CAGAGAGTAT TTTACCTTCC CAGCTTCCAA ATCCCAGGAT 
CGGATGGCTC CTCCTCAGAA CCAGTGGCCA AATTATGAGG AAAAGCCGCA TATGCACACA 
GATAGTAATC ATTCCAGTAT TGCAATTCAG CGTGTTACAC GTTCCCAAGA AGAACTTCGA 
GAAGATAAAG CTTACCAACT TGAGCGGCAT CGAATAGAGG CAGCTATGGA CCGAAAGTCT 
GATAGTGATA TGTGGATAAA TCAGAGCTCC TCACTGGACT CCAGTACCTC TAGCCAAGAG 
CATCTGAACC ATTCCTCTAA GTCGGTCACC CCTGCTTCTA CACTGACCAA AAGTGGCCCT 
GGCCGTTGGA AAACACCAGC AGCCATACCG GCCACCCCTG TGGCCATCTC CCAGCCAATC 
CGAACAGACC TGCCTCCGCC ACCCCCGCCA CCTCCAGTCC ACTATGCCGG TGATTTCGAT 
GGAATGTCCA TGGATTTGCC TCTCCCACCA CCCCCTTCCA CCAACCAGAT AGGGCTGCCG 
TCTGCACAGG TGGCTGCTGC CGAACGGAAA AAGAGAGAAG AACATCAGCG TTGGTATGAG 
AAGGAGAAGG CCCGCCTGGA GGAGGAGCGG GAGAGGAAGC GGAGAGAGCA GGAGAGGAAG 
TTGGGCCAGA TGCGCACTCA GTCCTTAAAC CCTGCTCCGT TTTCTCCCCT GACTGCACAG 
CAGATGAAGC CCGAAAAGCC TTCCACACTC CAGCGGCCAC AGGAAACAGT CATTCGGGAG 
CTGCAGCCTC AGCAGCAGCC CCGCACGATC GAGCGCAGAG ACTTGCAGTA CATCACAGTC 
AGCAAAGAGG AGCTTTCCTC GGGGGACAGT CTGTCCCCCG ACCCGTGGAA GCGGGACGCC 
AAGGAGAAGC TGGAGAAGCA GCAGCAGATG CACATCGTGG ACATGCTGAG CAAGGAGATC 
CAGGAGCTCC AGAGCAAACC GGACCGCAGC GCCGAGGAGA GCGACCGGCT GCGCAAGCTC 
ATGCTGGAGT GGCAGTTCCA GAAGAGACTC CAGGAGTCGA AGCAGAAGGA CGAAGATGAC 
GAGGAGGAGG AGGAGGACGA TGTGGACACC ATGCTGATCA TGCAGCGCCT GGAGGCTGAA 
CGAAGAGCGA GGTTGCAGGA CGAGGAGCGG AGGCGGCAGC AGCAGTTAGA AGAGATGCGC 
AAGCGGGAAG CGGAAGACCG AGCGAGGCAA GAGGAAGAGC GCCGGCGGCA GGAGGAGGAG 
CGAACAAAAC GAGACGCTGA AGAAAAGAGG CGACAGGAAG AAGGGTATTA CAGCCGCCTG 
GAAGCCGAGA GGCGCAGACA GCACGACGAG GCGGCGCGCA GGTTGCTGGA ACCCGAGGCG 
CCCGGTCTGT GCCGCCCTCC GCTTCCCCGG GACTACGAGC CCCCGTCCCC GTCCCCCGCG 
CCCGGCGCCC CTCCTCCCCC GCCTCAGCGA AACGCCTCCT ACCTCAAAAC ACAGGTCCTC 
TCCCCCGACT CGCTGTTCAC TGCCAAGTTT GTTGCATACA ATGAGGAGGA GGAGGAGGAG 
GACTGCAGCC TAGCAGGACC AAACTCTTAC CCAGGATCTA CTGGAGCAGC TGTTGGAGCC 
CATGACGCCT GTCGAGATGC AAAAGAGAAG CGCTCTAAAA GCCAAGATGC AGATTCACCT 
GGAAGTTCTG GGGCCCCTGA AAACTTGACA TTCAAGGAAC GCCAGCGTCT TTTTTCACAA 
GGTCAAGATG TATCCAATAA AGTGAAAGCT TCTCGTAAAT TAACAGAACT GGAGAATGAA 
CTGAACACAA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956651.2 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812140.1 Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X20, mRNA.

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CloneID	OPc87363
Clone ID Related Accession (Same CDS sequence)	XM_016956651.2 , XM_016956651.1
Accession Version	XM_016956651.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-03-19
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_036885.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Mar 20, 2018 this sequence version replaced XM_016956651.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Pan troglodytes Annotation Release 105 Annotation Version :: 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GAGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812140.1
CDS	257..5728
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956651.2
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes afadin, adherens junction formation factor (AFDN), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956651.2

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GAGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956651.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812140.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X20, mRNA.

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CloneID	OPc87363
Clone ID Related Accession (Same CDS sequence)	XM_016956651.2 , XM_016956651.1
Accession Version	XM_016956651.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5472bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GAGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812140.1
CDS	244..5715
Translation	MSAGGRDEERRKLADIIHHWNANRLDLFEISQPTEDLEFHGVMRFYFQDKAAGNFATKCIRVSSTATTQDVIETLAEKFRPDMRMLSSPKYSLYEVHVSGEERRLDIDEKPLVVQLNWNKDDREGRFVLKNENDAIPPKKAQSNGPEKQEKEGVIQNFKRTLSKKEKKEKKKREKEALRQASDKDDRPFQGEDVENSRLAAEVYKDMPETSFTRTISNPEVVMKRRRQQKLEKRMQEFRSSDGRPDSGGTLRIYADSLKPNIPYKTILLSTTDPADFAVAEALEKYGLEKENPKDYCIARVMLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956651.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956651.1

ORF Insert Sequence:

ATGTCGGCGG GCGGCCGTGA CGAGGAGCGG CGGAAGCTGG CCGACATCAT CCACCACTGG 
AACGCCAACC GGCTGGACCT GTTCGAGATC AGCCAGCCGA CCGAGGATTT GGAGTTCCAT 
GGAGTGATGA GATTTTATTT TCAAGATAAA GCTGCTGGAA ACTTTGCAAC AAAATGTATT 
CGGGTCTCTA GTACTGCCAC CACTCAAGAT GTAATCGAAA CGCTCGCGGA GAAATTTCGA 
CCTGATATGC GAATGCTGTC GTCTCCCAAG TATTCACTCT ATGAAGTGCA TGTCAGCGGA 
GAAGAAAGAA GATTGGATAT AGATGAGAAA CCTCTAGTTG TACAACTGAA TTGGAACAAA 
GATGATCGGG AAGGCAGATT TGTTCTTAAG AATGAGAATG ACGCCATTCC TCCTAAGAAG 
GCTCAAAGTA ATGGACCTGA AAAGCAGGAA AAAGAAGGGG TTATCCAGAA CTTCAAGAGA 
ACTCTCTCAA AGAAAGAAAA GAAGGAAAAA AAGAAGAGAG AAAAAGAGGC ATTGCGACAG 
GCATCTGATA AAGATGATAG ACCTTTCCAA GGGGAGGATG TTGAAAATTC TCGACTGGCT 
GCTGAGGTTT ACAAAGACAT GCCGGAAACC AGCTTTACTC GAACCATTTC TAATCCTGAG 
GTGGTTATGA AACGACGGAG GCAGCAAAAA TTGGAAAAGA GAATGCAGGA ATTTCGGAGC 
TCAGATGGGC GGCCTGATTC AGGTGGAACA TTGAGAATTT ATGCAGATAG TTTAAAACCA 
AATATTCCCT ACAAGACAAT CCTGCTGTCT ACTACAGATC CTGCAGACTT TGCTGTGGCT 
GAAGCTTTAG AGAAGTATGG TCTGGAAAAA GAAAACCCTA AGGATTACTG CATCGCCCGG 
GTTATGCTTC CTCCTGGAGC CCAGCATTCT GATGAAAAGG GTGCTAAAGA AATTATTCTT 
GATGATGATG AGTGTCCTTT ACAAATCTTC AGGGAATGGC CAAGTGACAA AGGGATTTTA 
GTCTTTCAAT TGAAGAGGAG GCCACCAGAC CACATCCCAA AGAAAACCAA GAAACACTTG 
GAAGGCAAGA CACCCAAGGG AAAGGAGAGA GCTGACGGGT CTGGCTATGG CTCCACCCTT 
CCTCCGGAGA AGCTGCCCTA TTTAGTAGAG TTAAGCCCAG GGAGAAGGAA TCACTTTGCC 
TACTACAACT ATCACACTTA CGAAGATGGT TCTGACTCTA GAGATAAGCC AAAGCTTTAC 
CGCCTTCAGT TAAGTGTTAC TGAAGTTGGG ACAGAAAAGT TGGATGACAA CTCTATCCAG 
TTGTTTGGCC CAGGAATTCA GCCCCATCAC TGTGACCTTA CCAACATGGA TGGAGTGGTC 
ACTGTGACGC CCAGAAGTAT GGACGCAGAA ACCTACGTGG AAGGCCAGCG CATCTCAGAA 
ACCACCATGC TGCAGAGTGG CATGAAAGTG CAGTTTGGGG CGTCCCATGT ATTTAAGTTT 
GTGGACCCCA GTCAGGATCA TGCTCTTGCA AAAAGATCTG TGGATGGAGG CCTGATGGTT 
AAGGGCCCAA GACATAAACC TGGAATTGTT CAGGAGACAA CTTTTGATTT GGGAGGAGAT 
ATTCATAGTG GGACAGCATT ACCGACAAGC AAGAGCACCA CTAGGCTGGA CAGCGACAGA 
GTGTCGTCTG CCTCTAGCAC AGCCGAGCGG GGAATGGTGA AGCCGATGAT CAGAGTAGAA 
CAGCAGCCAG ATTATCGCAG GCAAGAAAGC AGAACACAGG ATGTTTCTGG GCCTGAACTG 
ATACTACCTG CAAGCATTGA ATTCAGGGAA AGTTCTGAAG ATTCATTTTT GTCTGCCATT 
ATAAATTATA CTAATAGCTC TACAGTCCAC TTTAAGTTGT CCCCTACATA TGTATTATAT 
ATGGCATGCC GGTATGTATT GTCCAACCAG TACAGACCTG ACATCAGCCC TACAGAGCGC 
ACACATAAAG TCATTGCAGT CGTCAACAAG ATGGTGAGCA TGATGGAGGG CGTCATCCAG 
AAACAGAAGA ATATTGCAGG GGCACTTGCC TTCTGGATGG CAAATGCATC TGAACTTCTC 
AACTTCATTA AGCAAGACCG AGACCTTAGT CGGATCACAC TGGATGCTCA AGATGTTTTA 
GCACATTTGG TTCAAATGGC ATTTAAATAC TTGGTTCACT GTCTTCAATC AGAACTTAAT 
AATTACATGC CAGCCTTTCT AGATGACCCT GAAGAGAACA GTCTGCAAAG ACCAAAAATA 
GATGATGTGC TGCACACGCT CACAGGAGCC ATGTCCTTGC TACGACGCTG CAGAGTCAAT 
GCCGCCCTGA CCATCCAGCT CTTCTCTCAG CTCTTCCACT TCATCAATAT GTGGCTGTTC 
AATAGATTGG TGACCGACCC AGATTCGGGG CTGTGCTCCC ATTACTGGGG TGCAATTATC 
CGTCAGCAGT TGGGCCATAT TGAAGCCTGG GCTGAGAAGC AGGGGCTGGA ACTGGCTGCG 
GACTGTCATC TGAGCAGGAT CGTGCAGGCA ACGACTTTGC TTACCATGGA TAAGTATGCA 
CCTGATGACA TTCCAAATAT AAACAGCACC TGCTTTAAGT TAAATTCACT ACAACTTCAA 
GCCTTATTAC AGAACTATCA CTGTGCACCT GATGAGCCTT TTATCCCAAC GGATCTTATA 
GAAAATGTAG TGACTGTGGC TGAAAACACT GCCGATGAGC TGGCCCGCAG TGATGGAAGG 
GAAGTGCAGT TGGAGGAGGA TCCTGATCTG CAGCTGCCGT TTCTTTTGCC AGAAGATGGT 
TATTCTTGTG ATGTTGTCAG AAACATTCCA AATGGTTTAC AAGAATTTTT AGACCCTCTG 
TGCCAGAGAG GATTTTGCAG GTTAATTCCT CACACACGTT CACCAGGTAC TTGGACAATA 
TATTTTGAAG GTGCAGATTA TGAAAGTCAC CTTCTGCGTG AGAACACAGA GCTGGCTCAG 
CCTCTGAGGA AAGAACCTGA AATAATCACT GTGACCCTAA AAAAGCAGAA TGGAATGGGC 
CTTAGCATTG TTGCAGCAAA GGGTGCTGGT CAAGATAAAC TAGGAATCTA TGTGAAGTCG 
GTTGTGAAAG GAGGTGCTGC AGATGTGGAT GGACGTCTAG CTGCAGGTGA TCAGCTCCTC 
AGTGTGGATG GACGAAGTCT GGTTGGACTC TCTCAGGAAA GGGCAGCAGA ACTCATGACA 
AGAACAAGCT CTGTGGTGAC ACTGGAAGTA GCAAAGCAGG GTGCCATCTA CCACGGTCTG 
GCCACCCTTC TCAATCAGCC ATCCCCCATG ATGCAGAGAA TTTCAGATCG TCGTGGCTCA 
GGTAAACCCC GACCAAAGAG TGAAGGCTTT GAGCTCTATA ATAATTCAAC TCAAAATGGG 
TCTCCTGAGA GTCCTCAGCT GCCTTGGGCA GAATATAGTG AACCAAAGAA ATTGCCTGGT 
GATGACAGAC TGATGAAAAA TAGAGCTGAT CACCGTTCCA GCCCCAACGT AGCAAATCAG 
CCTCCTAGTC CTGGAGGGAA AAGTGCATAT GCCTCTGGAA CAACAGCGAA GATAACATCT 
GTCTCTACTG GAAACCTCTG CACTGAGGAG CAGACGCCTC CGCCTAGACC TGAAGCCTAC 
CCCATCCCCA CTCAGACGTA CACCAGAGAG TATTTTACCT TCCCAGCTTC CAAATCCCAG 
GATCGGATGG CTCCTCCTCA GAACCAGTGG CCAAATTATG AGGAAAAGCC GCATATGCAC 
ACAGATAGTA ATCATTCCAG TATTGCAATT CAGCGTGTTA CACGTTCCCA AGAAGAACTT 
CGAGAAGATA AAGCTTACCA ACTTGAGCGG CATCGAATAG AGGCAGCTAT GGACCGAAAG 
TCTGATAGTG ATATGTGGAT AAATCAGAGC TCCTCACTGG ACTCCAGTAC CTCTAGCCAA 
GAGCATCTGA ACCATTCCTC TAAGTCGGTC ACCCCTGCTT CTACACTGAC CAAAAGTGGC 
CCTGGCCGTT GGAAAACACC AGCAGCCATA CCGGCCACCC CTGTGGCCAT CTCCCAGCCA 
ATCCGAACAG ACCTGCCTCC GCCACCCCCG CCACCTCCAG TCCACTATGC CGGTGATTTC 
GATGGAATGT CCATGGATTT GCCTCTCCCA CCACCCCCTT CCACCAACCA GATAGGGCTG 
CCGTCTGCAC AGGTGGCTGC TGCCGAACGG AAAAAGAGAG AAGAACATCA GCGTTGGTAT 
GAGAAGGAGA AGGCCCGCCT GGAGGAGGAG CGGGAGAGGA AGCGGAGAGA GCAGGAGAGG 
AAGTTGGGCC AGATGCGCAC TCAGTCCTTA AACCCTGCTC CGTTTTCTCC CCTGACTGCA 
CAGCAGATGA AGCCCGAAAA GCCTTCCACA CTCCAGCGGC CACAGGAAAC AGTCATTCGG 
GAGCTGCAGC CTCAGCAGCA GCCCCGCACG ATCGAGCGCA GAGACTTGCA GTACATCACA 
GTCAGCAAAG AGGAGCTTTC CTCGGGGGAC AGTCTGTCCC CCGACCCGTG GAAGCGGGAC 
GCCAAGGAGA AGCTGGAGAA GCAGCAGCAG ATGCACATCG TGGACATGCT GAGCAAGGAG 
ATCCAGGAGC TCCAGAGCAA ACCGGACCGC AGCGCCGAGG AGAGCGACCG GCTGCGCAAG 
CTCATGCTGG AGTGGCAGTT CCAGAAGAGA CTCCAGGAGT CGAAGCAGAA GGACGAAGAT 
GACGAGGAGG AGGAGGAGGA CGATGTGGAC ACCATGCTGA TCATGCAGCG CCTGGAGGCT 
GAACGAAGAG CGAGGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGACCAAA CTCTTACCCA GGATCTACTG GAGCAGCTGT TGGAGCCCAT 
GACGCCTGTC GAGATGCAAA AGAGAAGCGC TCTAAAAGCC AAGATGCAGA TTCACCTGGA 
AGTTCTGGGG CCCCTGAAAA CTTGACATTC AAGGAACGCC AGCGTCTTTT TTCACAAGGT 
CAAGATGTAT CCAATAAAGT GAAAGCTTCT CGTAAATTAA CAGAACTGGA GAATGAACTG 
AACACAAAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

AFDN ( XM_016956655.1 ) cDNA ORF clone, Pan troglodytes(chimpanzee) -> XP_016812144.1 Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X24, mRNA.

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CloneID	OPc87367
Clone ID Related Accession (Same CDS sequence)	XM_016956655.1
Accession Version	XM_016956655.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-01
Organism	Pan troglodytes(chimpanzee)
Product	afadin isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006473.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Pan troglodytes Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_016812144.1
CDS	241..5103
Translation	MLPPGAQHSDEKGAKEIILDDDECPLQIFREWPSDKGILVFQLKRRPPDHIPKKTKKHLEGKTPKGKERADGSGYGSTLPPEKLPYLVELSPGRRNHFAYYNYHTYEDGSDSRDKPKLYRLQLSVTEVGTEKLDDNSIQLFGPGIQPHHCDLTNMDGVVTVTPRSMDAETYVEGQRISETTMLQSGMKVQFGASHVFKFVDPSQDHALAKRSVDGGLMVKGPRHKPGIVQETTFDLGGDIHSGTALPTSKSTTRLDSDRVSSASSTAERGMVKPMIRVEQQPDYRRQESRTQDVSGPELILPASIEFRESSEDSFLSAIINYTNSSTVHFKLSPTYVLYMACRYVLSNQYRPDISPTERTHKVIAVVNKMVSMMEGVIQEVDQVDQKQKNIAGALAFWMANASELLNFIKQDRDLSRITLDAQDVLAHLVQMAFKYLVHCLQSELNNYMPAFLDDPEENSLQRPKIDDVLHTLTGAMSLLRRCRVNAALTIQLFSQLFHFINMWLFNRLVTDPDSGLCSHYWGAIIRQQLGHIEAWAEKQGLELAADCHLSRIVQATTLLTMDKYAPDDIPNINSTCFKLNSLQLQALLQNYHCAPDEPFIPTDLIENVVTVAENTADELARSDGREVQLEEDPDLQLPFLLPEDGYSCDVVRNIPNGLQEFLDPLCQRGFCRLIPHTRSPGTWTIYFEGADYESHLLRENTELAQPLRKEPEIITVTLKKQNGMGLSIVAAKGAGQDKLGIYVKSVVKGGAADVDGRLAAGDQLLSVDGRSLVGLSQERAAELMTRTSSVVTLEVAKQGAIYHGLATLLNQPSPMMQRISDRRGSGKPRPKSEGFELYNNSTQNGSPESPQLPWAEYSEPKKLPGDDRLMKNRADHRSSPNVANQPPSPGGKSAYASGTTAKITSVSTGNLCTEEQTPPPRPEAYPIPTQTYTREYFTFPASKSQDRMAPPQNQWPNYEEKPHMHTDSNHSSIAIQRVTRSQEELREDKAYQLERHRIEAAMDRKSDSDMWINQSSSLDSSTSSQEHLNHSSKSVTPASTLTKSGPGRWKTPAAIPATPVAISQPIRTDLPPPPPPPPVHYAGDFDGMSMDLPLPPPPSTNQIGLPSAQVAAAERKKREEHQRWYEKEKARLEEERERKRREQERKLGQMRTQSLNPAPFSPLTAQQMKPEKPSTLQRPQETVIRELQPQQQPRTIERRDLQYITVSKEELSSGDSLSPDPWKRDAKEKLEKQQQMHIVDMLSKEIQELQSKPDRSAEESDRLRKLMLEWQFQKRLQESKQKDEDDEEEEEDDVDTMLIMQRLEAERRARQTAMPAISVLDLLQDEERRRQQQLEEMRKREAEDRARQEEERRRQEEERTKRDAEEKRRQEEGYYSRLEAERRRQHDEAARRLLEPEAPGLCRPPLPRDYEPPSPSPAPGAPPPPPQRNASYLKTQVLSPDSLFTAKFVAYNEEEEEEDCSLAGQDKYSSTRKSHGDLLPAPLKPRPPPCQPRPASDGIFLSNSFQPPSAKANSTAHKKGQPPPPPKKSSSYHPSHCKGRGPNSYPGSTGAAVGAHDACRDAKEKRSKSQDADSPGSSGAPENLTFKERQRLFSQGQDVSNKVKASRKLTELENELNTK

Target ORF information:

RefSeq Version	XM_016956655.1
Organism	Pan troglodytes(chimpanzee)
Definition	Pan troglodytes myeloid/lymphoid or mixed-lineage leukemia; translocated to, 4 (MLLT4), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_016956655.1

ORF Insert Sequence:

ATGCTTCCTC CTGGAGCCCA GCATTCTGAT GAAAAGGGTG CTAAAGAAAT TATTCTTGAT 
GATGATGAGT GTCCTTTACA AATCTTCAGG GAATGGCCAA GTGACAAAGG GATTTTAGTC 
TTTCAATTGA AGAGGAGGCC ACCAGACCAC ATCCCAAAGA AAACCAAGAA ACACTTGGAA 
GGCAAGACAC CCAAGGGAAA GGAGAGAGCT GACGGGTCTG GCTATGGCTC CACCCTTCCT 
CCGGAGAAGC TGCCCTATTT AGTAGAGTTA AGCCCAGGGA GAAGGAATCA CTTTGCCTAC 
TACAACTATC ACACTTACGA AGATGGTTCT GACTCTAGAG ATAAGCCAAA GCTTTACCGC 
CTTCAGTTAA GTGTTACTGA AGTTGGGACA GAAAAGTTGG ATGACAACTC TATCCAGTTG 
TTTGGCCCAG GAATTCAGCC CCATCACTGT GACCTTACCA ACATGGATGG AGTGGTCACT 
GTGACGCCCA GAAGTATGGA CGCAGAAACC TACGTGGAAG GCCAGCGCAT CTCAGAAACC 
ACCATGCTGC AGAGTGGCAT GAAAGTGCAG TTTGGGGCGT CCCATGTATT TAAGTTTGTG 
GACCCCAGTC AGGATCATGC TCTTGCAAAA AGATCTGTGG ATGGAGGCCT GATGGTTAAG 
GGCCCAAGAC ATAAACCTGG AATTGTTCAG GAGACAACTT TTGATTTGGG AGGAGATATT 
CATAGTGGGA CAGCATTACC GACAAGCAAG AGCACCACTA GGCTGGACAG CGACAGAGTG 
TCGTCTGCCT CTAGCACAGC CGAGCGGGGA ATGGTGAAGC CGATGATCAG AGTAGAACAG 
CAGCCAGATT ATCGCAGGCA AGAAAGCAGA ACACAGGATG TTTCTGGGCC TGAACTGATA 
CTACCTGCAA GCATTGAATT CAGGGAAAGT TCTGAAGATT CATTTTTGTC TGCCATTATA 
AATTATACTA ATAGCTCTAC AGTCCACTTT AAGTTGTCCC CTACATATGT ATTATATATG 
GCATGCCGGT ATGTATTGTC CAACCAGTAC AGACCTGACA TCAGCCCTAC AGAGCGCACA 
CATAAAGTCA TTGCAGTCGT CAACAAGATG GTGAGCATGA TGGAGGGCGT CATCCAGGAA 
GTAGACCAGG TTGACCAGAA ACAGAAGAAT ATTGCAGGGG CACTTGCCTT CTGGATGGCA 
AATGCATCTG AACTTCTCAA CTTCATTAAG CAAGACCGAG ACCTTAGTCG GATCACACTG 
GATGCTCAAG ATGTTTTAGC ACATTTGGTT CAAATGGCAT TTAAATACTT GGTTCACTGT 
CTTCAATCAG AACTTAATAA TTACATGCCA GCCTTTCTAG ATGACCCTGA AGAGAACAGT 
CTGCAAAGAC CAAAAATAGA TGATGTGCTG CACACGCTCA CAGGAGCCAT GTCCTTGCTA 
CGACGCTGCA GAGTCAATGC CGCCCTGACC ATCCAGCTCT TCTCTCAGCT CTTCCACTTC 
ATCAATATGT GGCTGTTCAA TAGATTGGTG ACCGACCCAG ATTCGGGGCT GTGCTCCCAT 
TACTGGGGTG CAATTATCCG TCAGCAGTTG GGCCATATTG AAGCCTGGGC TGAGAAGCAG 
GGGCTGGAAC TGGCTGCGGA CTGTCATCTG AGCAGGATCG TGCAGGCAAC GACTTTGCTT 
ACCATGGATA AGTATGCACC TGATGACATT CCAAATATAA ACAGCACCTG CTTTAAGTTA 
AATTCACTAC AACTTCAAGC CTTATTACAG AACTATCACT GTGCACCTGA TGAGCCTTTT 
ATCCCAACGG ATCTTATAGA AAATGTAGTG ACTGTGGCTG AAAACACTGC CGATGAGCTG 
GCCCGCAGTG ATGGAAGGGA AGTGCAGTTG GAGGAGGATC CTGATCTGCA GCTGCCGTTT 
CTTTTGCCAG AAGATGGTTA TTCTTGTGAT GTTGTCAGAA ACATTCCAAA TGGTTTACAA 
GAATTTTTAG ACCCTCTGTG CCAGAGAGGA TTTTGCAGGT TAATTCCTCA CACACGTTCA 
CCAGGTACTT GGACAATATA TTTTGAAGGT GCAGATTATG AAAGTCACCT TCTGCGTGAG 
AACACAGAGC TGGCTCAGCC TCTGAGGAAA GAACCTGAAA TAATCACTGT GACCCTAAAA 
AAGCAGAATG GAATGGGCCT TAGCATTGTT GCAGCAAAGG GTGCTGGTCA AGATAAACTA 
GGAATCTATG TGAAGTCGGT TGTGAAAGGA GGTGCTGCAG ATGTGGATGG ACGTCTAGCT 
GCAGGTGATC AGCTCCTCAG TGTGGATGGA CGAAGTCTGG TTGGACTCTC TCAGGAAAGG 
GCAGCAGAAC TCATGACAAG AACAAGCTCT GTGGTGACAC TGGAAGTAGC AAAGCAGGGT 
GCCATCTACC ACGGTCTGGC CACCCTTCTC AATCAGCCAT CCCCCATGAT GCAGAGAATT 
TCAGATCGTC GTGGCTCAGG TAAACCCCGA CCAAAGAGTG AAGGCTTTGA GCTCTATAAT 
AATTCAACTC AAAATGGGTC TCCTGAGAGT CCTCAGCTGC CTTGGGCAGA ATATAGTGAA 
CCAAAGAAAT TGCCTGGTGA TGACAGACTG ATGAAAAATA GAGCTGATCA CCGTTCCAGC 
CCCAACGTAG CAAATCAGCC TCCTAGTCCT GGAGGGAAAA GTGCATATGC CTCTGGAACA 
ACAGCGAAGA TAACATCTGT CTCTACTGGA AACCTCTGCA CTGAGGAGCA GACGCCTCCG 
CCTAGACCTG AAGCCTACCC CATCCCCACT CAGACGTACA CCAGAGAGTA TTTTACCTTC 
CCAGCTTCCA AATCCCAGGA TCGGATGGCT CCTCCTCAGA ACCAGTGGCC AAATTATGAG 
GAAAAGCCGC ATATGCACAC AGATAGTAAT CATTCCAGTA TTGCAATTCA GCGTGTTACA 
CGTTCCCAAG AAGAACTTCG AGAAGATAAA GCTTACCAAC TTGAGCGGCA TCGAATAGAG 
GCAGCTATGG ACCGAAAGTC TGATAGTGAT ATGTGGATAA ATCAGAGCTC CTCACTGGAC 
TCCAGTACCT CTAGCCAAGA GCATCTGAAC CATTCCTCTA AGTCGGTCAC CCCTGCTTCT 
ACACTGACCA AAAGTGGCCC TGGCCGTTGG AAAACACCAG CAGCCATACC GGCCACCCCT 
GTGGCCATCT CCCAGCCAAT CCGAACAGAC CTGCCTCCGC CACCCCCGCC ACCTCCAGTC 
CACTATGCCG GTGATTTCGA TGGAATGTCC ATGGATTTGC CTCTCCCACC ACCCCCTTCC 
ACCAACCAGA TAGGGCTGCC GTCTGCACAG GTGGCTGCTG CCGAACGGAA AAAGAGAGAA 
GAACATCAGC GTTGGTATGA GAAGGAGAAG GCCCGCCTGG AGGAGGAGCG GGAGAGGAAG 
CGGAGAGAGC AGGAGAGGAA GTTGGGCCAG ATGCGCACTC AGTCCTTAAA CCCTGCTCCG 
TTTTCTCCCC TGACTGCACA GCAGATGAAG CCCGAAAAGC CTTCCACACT CCAGCGGCCA 
CAGGAAACAG TCATTCGGGA GCTGCAGCCT CAGCAGCAGC CCCGCACGAT CGAGCGCAGA 
GACTTGCAGT ACATCACAGT CAGCAAAGAG GAGCTTTCCT CGGGGGACAG TCTGTCCCCC 
GACCCGTGGA AGCGGGACGC CAAGGAGAAG CTGGAGAAGC AGCAGCAGAT GCACATCGTG 
GACATGCTGA GCAAGGAGAT CCAGGAGCTC CAGAGCAAAC CGGACCGCAG CGCCGAGGAG 
AGCGACCGGC TGCGCAAGCT CATGCTGGAG TGGCAGTTCC AGAAGAGACT CCAGGAGTCG 
AAGCAGAAGG ACGAAGATGA CGAGGAGGAG GAGGAGGACG ATGTGGACAC CATGCTGATC 
ATGCAGCGCC TGGAGGCTGA ACGAAGAGCG AGGCAGACTG CTATGCCAGC AATCTCTGTT 
CTAGATTTGT TGCAGGACGA GGAGCGGAGG CGGCAGCAGC AGTTAGAAGA GATGCGCAAG 
CGGGAAGCGG AAGACCGAGC GAGGCAAGAG GAAGAGCGCC GGCGGCAGGA GGAGGAGCGA 
ACAAAACGAG ACGCTGAAGA AAAGAGGCGA CAGGAAGAAG GGTATTACAG CCGCCTGGAA 
GCCGAGAGGC GCAGACAGCA CGACGAGGCG GCGCGCAGGT TGCTGGAACC CGAGGCGCCC 
GGTCTGTGCC GCCCTCCGCT TCCCCGGGAC TACGAGCCCC CGTCCCCGTC CCCCGCGCCC 
GGCGCCCCTC CTCCCCCGCC TCAGCGAAAC GCCTCCTACC TCAAAACACA GGTCCTCTCC 
CCCGACTCGC TGTTCACTGC CAAGTTTGTT GCATACAATG AGGAGGAGGA GGAGGAGGAC 
TGCAGCCTAG CAGGTCAGGA TAAGTACTCC AGCACAAGAA AGTCTCATGG GGACCTTCTT 
CCTGCCCCTC TTAAACCACG GCCACCCCCC TGCCAGCCAC GCCCGGCCTC CGATGGCATC 
TTTCTCTCCA ACTCATTCCA GCCACCCTCA GCCAAAGCCA ACAGTACTGC TCACAAAAAA 
GGCCAGCCCC CGCCCCCTCC AAAAAAATCC AGCTCCTACC ATCCCAGCCA CTGCAAAGGT 
AGAGGACCAA ACTCTTACCC AGGATCTACT GGAGCAGCTG TTGGAGCCCA TGACGCCTGT 
CGAGATGCAA AAGAGAAGCG CTCTAAAAGC CAAGATGCAG ATTCACCTGG AAGTTCTGGG 
GCCCCTGAAA ACTTGACATT CAAGGAACGC CAGCGTCTTT TTTCACAAGG TCAAGATGTA 
TCCAATAAAG TGAAAGCTTC TCGTAAATTA ACAGAACTGG AGAATGAACT GAACACAAAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

AFDN cDNA ORF clone, Pan troglodytes(chimpanzee)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information: