IL12B cDNA ORF clone, Gallus gallus(chicken)

Gene
Clones
gRNAs

Summary

Gene Symbol	IL12B
Entrez Gene ID	404671
Full Name	interleukin 12B
Synonyms	IL-12B,IL-12p40
General protein information	Preferred Names interleukin 12B Names interleukin-12 subunit beta IL-12 p40 subunit interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocyte maturation factor 2, p40) interleukin-12 beta chain interleukin-12 p40 subunit interleukin-12p40
Gene Type	protein-coding
Organism	Gallus gallus(chicken)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015293641.1	XP_015149127.1	interleukin-12 subunit beta isoform X1
NM_213571.1	NP_998736.1	interleukin-12 subunit beta precursor
XM_015293640.1	XP_015149126.1	interleukin-12 subunit beta isoform X1
XM_015293641.2	XP_015149127.1	interleukin-12 subunit beta isoform X1
XM_025154822.1	XP_025010590.1	interleukin-12 subunit beta isoform X1
XM_015293642.2	XP_015149128.1	interleukin-12 subunit beta isoform X2
XM_015293642.1	XP_015149128.1	interleukin-12 subunit beta isoform X2

Pathways from BioSystems

Homologies

	IL12B	NP_001038190.1
Bos taurus (cattle)	IL12B	NP_776781.1
Homo sapiens (human)	IL12B	NP_002178.2
Gallus gallus (chicken)	IL12B	NP_998736.1
Canis lupus familiaris (dog)	IL12B	NP_001003292.1
Mus musculus (house mouse)	Il12b	NP_032378.1
Danio rerio (zebrafish)	il12ba	NP_001007109.1
Pan troglodytes (chimpanzee)	IL12B	XP_527101.2
Rattus norvegicus (Norway rat)	Il12b	NP_072133.1
Danio rerio (zebrafish)	il12bb	XP_002666092.2

Gene Ontology
Bibliography

Related articles in PubMed

Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex.
Truong AD, Hoang CT, Hong Y, Lee J, Lee K, Lillehoj HS, Hong YH
Molecular immunology9254-67(2017 Dec)

Arsenic Trioxide Attenuates NF-?B and Cytokine mRNA Levels in the Livers of Cocks.
Zhang K, Zhao P, Guo G, Guo Y, Tian L, Sun X, Li S, He Y, Sun Y, Chai H, Zhang W, Xing M
Biological trace element research170(2)432-7(2016 Apr)

A high serum interleukin-12p40 level prior to Kasai surgery predict a favourable outcome in children with biliary atresia.
Wu JF, Kao PC, Chen HL, Lai HS, Hsu HY, Chang MH, Ni YH
Liver international : official journal of the International Association for the Study of the Liver32(10)1557-63(2012 Nov)

Identification and molecular cloning of functional chicken IL-12.
Degen WG, van Daal N, van Zuilekom HI, Burnside J, Schijns VE
Journal of immunology (Baltimore, Md. : 1950)172(7)4371-80(2004 Apr)

Avian interleukin-12beta (p40): cloning and characterization of the cDNA and gene.
Balu S, Kaiser P
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research23(12)699-707(2003 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

these results indicate that ChIL-23 is a member of the IL-12 family, has proinflammatory properties related to IL-23R and IL-12Rbeta1 receptor expression, and activates the JAK/STAT signaling pathway that results in the interaction of ChIL-23alpha with ChIL-12p40 to form the novel ChIL-23 complex.
Title: Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex.

The results showed that the expression levels of IL-6, IL-8, TNF-alpha, and NF-kappaB which seemed to be a critical mediator in the inflammatory response tended to increase in the birds chronically treated with As2O3. However, the mRNA expression levels of IL-4, IL-12beta, and IL-1beta were decreased in the experiment.
Title: Arsenic Trioxide Attenuates NF-?B and Cytokine mRNA Levels in the Livers of Cocks.

A high serum interleukin-12p40 level prior to Kasai surgery predict a favourable outcome in children with biliary atresia.
Title: A high serum interleukin-12p40 level prior to Kasai surgery predict a favourable outcome in children with biliary atresia.

The high degree of functional similarity between chicken IL-12p40 and that of higher vertebrates, despite poor sequence homology, illustrates the conservation of IL-12 since the evolutionary dichotomy of birds and mammals >300 million years ago.
Title: Identification and molecular cloning of functional chicken IL-12.

molecular cloning; IL-12 p40 mRNA expression was identified in a wide variety of tissues and in B, T, and macrophage cell lines
Title: Avian interleukin-12beta (p40): cloning and characterization of the cDNA and gene.

The following IL12B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the IL12B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OGa23831	NM_213571.1 Latest version!	Gallus gallus interleukin 12B (IL12B), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa23831	XM_015293641.2 Latest version!	Gallus gallus interleukin 12B (IL12B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa23831	XM_015293640.1 Latest version!	Gallus gallus interleukin 12B (IL12B), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa23831	XM_025154822.1 Latest version!	Gallus gallus interleukin 12B (IL12B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa41832	XM_015293642.2 Latest version!	Gallus gallus interleukin 12B (IL12B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
View Old Accession Versions >>
OGa23831	XM_015293641.1	Gallus gallus interleukin 12B (IL12B), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
OGa41832	XM_015293642.1	Gallus gallus interleukin 12B (IL12B), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

IL12B ( NM_213571.1 ) cDNA ORF clone, Gallus gallus(chicken) -> NP_998736.1 Gallus gallus interleukin 12B (IL12B), mRNA.

Price & Availability↑

CloneID	OGa23831
Clone ID Related Accession (Same CDS sequence)	XM_015293641.1 , NM_213571.1 , XM_015293640.1 , XM_015293641.2 , XM_025154822.1
Accession Version	NM_213571.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-20
Organism	Gallus gallus(chicken)
Product	interleukin-12 subunit beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AY262752.1. ##Evidence-Data-START## Transcript exon combination :: AY262752.1, DQ202328.1 [ECO:0000332] ##Evidence-Data-END##

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998736.1
CDS	1..948
Translation	MSHLLFALLSLLSFAALLEAQWKLRENVYVIESEWNDETPAKKVKLTCDTSDEALPVYWKKGTELKGTGKTLTTEVKEFPDAGNYTCLSAKTHEIISYSFFLITKVDSNGQMIRSILKSYKEPSKTFLKCEAKNYSGIFTCSWMTENESPSVKFTIRSLKGSQGDVTCSSPVARTDKSVTEYTAQCQKENYCPFAEEHQPTEMFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYASTNGTVTWTYPKTWSTPKSYFPLTFRVKVESTKKYKSKVYDADEQSIQIPKTGPKDKISVQARDRYYNSSWSEWSTLCR

Target ORF information:

RefSeq Version	NM_213571.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interleukin 12B (IL12B), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_213571.1

ORF Insert Sequence:

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL12B ( XM_015293641.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149127.1 Gallus gallus interleukin 12B (IL12B), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OGa23831
Clone ID Related Accession (Same CDS sequence)	XM_015293641.1 , NM_213571.1 , XM_015293640.1 , XM_015293641.2 , XM_025154822.1
Accession Version	XM_015293641.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	interleukin-12 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455954.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149127.1
CDS	2794..3741
Translation	MSHLLFALLSLLSFAALLEAQWKLRENVYVIESEWNDETPAKKVKLTCDTSDEALPVYWKKGTELKGTGKTLTTEVKEFPDAGNYTCLSAKTHEIISYSFFLITKVDSNGQMIRSILKSYKEPSKTFLKCEAKNYSGIFTCSWMTENESPSVKFTIRSLKGSQGDVTCSSPVARTDKSVTEYTAQCQKENYCPFAEEHQPTEMFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYASTNGTVTWTYPKTWSTPKSYFPLTFRVKVESTKKYKSKVYDADEQSIQIPKTGPKDKISVQARDRYYNSSWSEWSTLCR

Target ORF information:

RefSeq Version	XM_015293641.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interleukin 12B (IL12B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293641.1

ORF Insert Sequence:

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL12B ( XM_015293640.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149126.1 Gallus gallus interleukin 12B (IL12B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa23831
Clone ID Related Accession (Same CDS sequence)	XM_015293641.1 , NM_213571.1 , XM_015293640.1 , XM_015293641.2 , XM_025154822.1
Accession Version	XM_015293640.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	interleukin-12 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455954.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149126.1
CDS	2619..3566
Translation	MSHLLFALLSLLSFAALLEAQWKLRENVYVIESEWNDETPAKKVKLTCDTSDEALPVYWKKGTELKGTGKTLTTEVKEFPDAGNYTCLSAKTHEIISYSFFLITKVDSNGQMIRSILKSYKEPSKTFLKCEAKNYSGIFTCSWMTENESPSVKFTIRSLKGSQGDVTCSSPVARTDKSVTEYTAQCQKENYCPFAEEHQPTEMFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYASTNGTVTWTYPKTWSTPKSYFPLTFRVKVESTKKYKSKVYDADEQSIQIPKTGPKDKISVQARDRYYNSSWSEWSTLCR

Target ORF information:

RefSeq Version	XM_015293640.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interleukin 12B (IL12B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293640.1

ORF Insert Sequence:

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL12B ( XM_015293641.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149127.1 Gallus gallus interleukin 12B (IL12B), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OGa23831
Clone ID Related Accession (Same CDS sequence)	XM_015293641.1 , NM_213571.1 , XM_015293640.1 , XM_015293641.2 , XM_025154822.1
Accession Version	XM_015293641.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	interleukin-12 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293641.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149127.1
CDS	2793..3740
Translation	MSHLLFALLSLLSFAALLEAQWKLRENVYVIESEWNDETPAKKVKLTCDTSDEALPVYWKKGTELKGTGKTLTTEVKEFPDAGNYTCLSAKTHEIISYSFFLITKVDSNGQMIRSILKSYKEPSKTFLKCEAKNYSGIFTCSWMTENESPSVKFTIRSLKGSQGDVTCSSPVARTDKSVTEYTAQCQKENYCPFAEEHQPTEMFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYASTNGTVTWTYPKTWSTPKSYFPLTFRVKVESTKKYKSKVYDADEQSIQIPKTGPKDKISVQARDRYYNSSWSEWSTLCR

Target ORF information:

RefSeq Version	XM_015293641.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interleukin 12B (IL12B), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293641.2

ORF Insert Sequence:

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL12B ( XM_025154822.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_025010590.1 Gallus gallus interleukin 12B (IL12B), transcript variant X2, mRNA.

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CloneID	OGa23831
Clone ID Related Accession (Same CDS sequence)	XM_015293641.1 , NM_213571.1 , XM_015293640.1 , XM_015293641.2 , XM_025154822.1
Accession Version	XM_025154822.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	interleukin-12 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025010590.1
CDS	763..1710
Translation	MSHLLFALLSLLSFAALLEAQWKLRENVYVIESEWNDETPAKKVKLTCDTSDEALPVYWKKGTELKGTGKTLTTEVKEFPDAGNYTCLSAKTHEIISYSFFLITKVDSNGQMIRSILKSYKEPSKTFLKCEAKNYSGIFTCSWMTENESPSVKFTIRSLKGSQGDVTCSSPVARTDKSVTEYTAQCQKENYCPFAEEHQPTEMFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYASTNGTVTWTYPKTWSTPKSYFPLTFRVKVESTKKYKSKVYDADEQSIQIPKTGPKDKISVQARDRYYNSSWSEWSTLCR

Target ORF information:

RefSeq Version	XM_025154822.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interleukin 12B (IL12B), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025154822.1

ORF Insert Sequence:

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL12B ( XM_015293642.2 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149128.1 Gallus gallus interleukin 12B (IL12B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa41832
Clone ID Related Accession (Same CDS sequence)	XM_015293642.2 , XM_015293642.1
Accession Version	XM_015293642.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-16
Organism	Gallus gallus(chicken)
Product	interleukin-12 subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006100.5) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On May 17, 2018 this sequence version replaced XM_015293642.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Gallus gallus Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149128.1
CDS	2618..3565
Translation	MSHLLFALLSLLSFAALLEAQWKLRENVYVIESEWNDETPAKKVKLTCDTSDEALPVYWKKGTELKGTGKTLTTEVKEFPDAGNYTCLSAKTHEIISYSFFLITKVDSNGQMIRSILKSYKEPSKTFLKCEAKNYSGIFTCSWMTENESPSVKFTIRSLKGSQGDVTCSSPVARTDKSVTEYTAQCQKENYCPFAEEHQPTEMFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYASTNGTVTWTYPKTWSTPKSYFPLTFRVKVESTKKYKSKVYDADEQSIQIPKTGPKDKISVQARDRYYNSSWSEWSTLCR

Target ORF information:

RefSeq Version	XM_015293642.2
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interleukin 12B (IL12B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293642.2

ORF Insert Sequence:

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

IL12B ( XM_015293642.1 ) cDNA ORF clone, Gallus gallus(chicken) -> XP_015149128.1 Gallus gallus interleukin 12B (IL12B), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OGa41832
Clone ID Related Accession (Same CDS sequence)	XM_015293642.2 , XM_015293642.1
Accession Version	XM_015293642.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 948bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-03
Organism	Gallus gallus(chicken)
Product	interleukin-12 subunit beta isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NT_455954.1) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Gallus gallus Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015149128.1
CDS	2619..3566
Translation	MSHLLFALLSLLSFAALLEAQWKLRENVYVIESEWNDETPAKKVKLTCDTSDEALPVYWKKGTELKGTGKTLTTEVKEFPDAGNYTCLSAKTHEIISYSFFLITKVDSNGQMIRSILKSYKEPSKTFLKCEAKNYSGIFTCSWMTENESPSVKFTIRSLKGSQGDVTCSSPVARTDKSVTEYTAQCQKENYCPFAEEHQPTEMFLEVIDEVEYENYTSSFFIRDIIKPDPPQCQYASTNGTVTWTYPKTWSTPKSYFPLTFRVKVESTKKYKSKVYDADEQSIQIPKTGPKDKISVQARDRYYNSSWSEWSTLCR

Target ORF information:

RefSeq Version	XM_015293642.1
Organism	Gallus gallus(chicken)
Definition	Gallus gallus interleukin 12B (IL12B), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015293642.1

ORF Insert Sequence:

ATGTCTCACC TGCTATTTGC CTTACTTTCA TTACTTTCCT TTGCTGCCCT TCTGGAAGCA 
CAGTGGAAAC TTAGAGAGAA TGTGTATGTC ATAGAATCTG AGTGGAACGA TGAGACACCA 
GCTAAAAAAG TGAAGCTCAC CTGTGACACA TCTGATGAAG CACTGCCAGT TTACTGGAAA 
AAGGGAACAG AACTGAAAGG AACTGGAAAG ACTCTGACCA CCGAAGTGAA GGAGTTCCCA 
GATGCTGGCA ACTACACCTG CCTGTCTGCT AAGACCCACG AGATTATCAG CTACAGTTTC 
TTTCTCATAA CTAAAGTAGA CTCCAATGGG CAAATGATAC GGTCAATTCT GAAAAGCTAT 
AAAGAGCCAA GCAAGACGTT CTTAAAATGT GAGGCAAAGA ACTACTCTGG AATTTTCACA 
TGTTCATGGA TGACAGAAAA TGAGAGTCCA AGTGTGAAGT TCACAATTAG GAGCCTAAAA 
GGCTCTCAAG GAGATGTAAC CTGCAGCAGC CCTGTGGCTC GCACTGATAA ATCTGTGACT 
GAATACACTG CCCAGTGCCA GAAGGAAAAC TACTGTCCAT TTGCCGAAGA GCACCAGCCG 
ACTGAGATGT TCCTGGAGGT CATTGATGAG GTGGAATATG AGAACTACAC TAGTAGCTTC 
TTCATCAGAG ATATCATAAA GCCAGACCCA CCTCAATGTC AGTATGCAAG CACAAATGGA 
ACTGTGACCT GGACATATCC CAAGACCTGG AGCACACCGA AGTCCTACTT CCCTTTGACT 
TTCAGGGTCA AAGTTGAAAG CACAAAGAAA TACAAAAGCA AGGTTTATGA TGCTGATGAG 
CAGTCTATTC AGATTCCAAA GACTGGGCCA AAAGACAAGA TCTCTGTGCA GGCCAGGGAT 
CGCTATTACA ACTCATCCTG GAGTGAGTGG TCCACGCTTT GCAGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Functional analyses of the interaction of chicken interleukin 23 subunit p19 with IL-12 subunit p40 to form the IL-23 complex.
Molecular immunology9254-67(2017 Dec)
Truong AD,Hoang CT,Hong Y,Lee J,Lee K,Lillehoj HS,Hong YH

Arsenic Trioxide Attenuates NF-?B and Cytokine mRNA Levels in the Livers of Cocks.
Biological trace element research170(2)432-7(2016 Apr)
Zhang K,Zhao P,Guo G,Guo Y,Tian L,Sun X,Li S,He Y,Sun Y,Chai H,Zhang W,Xing M

A high serum interleukin-12p40 level prior to Kasai surgery predict a favourable outcome in children with biliary atresia.
Liver international : official journal of the International Association for the Study of the Liver32(10)1557-63(2012 Nov)
Wu JF,Kao PC,Chen HL,Lai HS,Hsu HY,Chang MH,Ni YH

Identification and molecular cloning of functional chicken IL-12.
Journal of immunology (Baltimore, Md. : 1950)172(7)4371-80(2004 Apr)
Degen WG,van Daal N,van Zuilekom HI,Burnside J,Schijns VE

Avian interleukin-12beta (p40): cloning and characterization of the cDNA and gene.
Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research23(12)699-707(2003 Dec)
Balu S,Kaiser P

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IL12B cDNA ORF clone, Gallus gallus(chicken)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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