Il12b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Il12b
Entrez Gene ID	64546
Full Name	interleukin 12B
Synonyms	Il12
General protein information	Preferred Names interleukin 12B Names interleukin-12 subunit beta interleukin-12 p40
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q21
Summary	a subunit of interleukin 12 which is a cytokine that acts on T and natural killer cells [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006246147.3	XP_006246209.1	interleukin-12 subunit beta isoform X1
NM_022611.1	NP_072133.1	interleukin-12 subunit beta precursor

Pathways from BioSystems

Homologies

	IL12B	NP_001038190.1
Bos taurus (cattle)	IL12B	NP_776781.1
Homo sapiens (human)	IL12B	NP_002178.2
Gallus gallus (chicken)	IL12B	NP_998736.1
Canis lupus familiaris (dog)	IL12B	NP_001003292.1
Mus musculus (house mouse)	Il12b	NP_032378.1
Danio rerio (zebrafish)	il12ba	NP_001007109.1
Pan troglodytes (chimpanzee)	IL12B	XP_527101.2
Rattus norvegicus (Norway rat)	Il12b	NP_072133.1
Danio rerio (zebrafish)	il12bb	XP_002666092.2

Gene Ontology
Bibliography

Related articles in PubMed

A molecular threshold for effector CD8(+) T cell differentiation controlled by transcription factors Blimp-1 and T-bet.
Xin A, Masson F, Liao Y, Preston S, Guan T, Gloury R, Olshansky M, Lin JX, Li P, Speed TP, Smyth GK, Ernst M, Leonard WJ, Pellegrini M, Kaech SM, Nutt SL, Shi W, Belz GT, Kallies A
Nature immunology17(4)422-32(2016 Apr)

Interaction of TLR-IFN and HLA polymorphisms on susceptibility of chronic HBV infection in Southwest Han Chinese.
He D, Tao S, Guo S, Li M, Wu J, Huang H, Guo X, Yan G, Zhu P, Wang Y
Liver international : official journal of the International Association for the Study of the Liver35(8)1941-9(2015 Aug)

Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.
Yang S, Wang B, Humphries F, Jackson R, Healy ME, Bergin R, Aviello G, Hall B, McNamara D, Darby T, Quinlan A, Shanahan F, Melgar S, Fallon PG, Moynagh PN
Nature immunology14(9)927-36(2013 Sep)

Glucocorticoids target suppressor of cytokine signaling 1 (SOCS1) and type 1 interferons to regulate Toll-like receptor-induced STAT1 activation.
Bhattacharyya S, Zhao Y, Kay TW, Muglia LJ
Proceedings of the National Academy of Sciences of the United States of America108(23)9554-9(2011 Jun)

H-ras and N-ras are dispensable for T-cell development and activation but critical for protective Th1 immunity.
Iborra S, Soto M, Stark-Aroeira L, Castellano E, Alarc?n B, Alonso C, Santos E, Fern?ndez-Malav? E
Blood117(19)5102-11(2011 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

There is a common program of effector T cell differentiation that is regulated by IL-2 and IL-12 signaling and the combined activities of the transcriptional regulators Blimp-1 and T-bet.
Title: A molecular threshold for effector CD8(+) T cell differentiation controlled by transcription factors Blimp-1 and T-bet.

Data show that treated rats exhibited the lowest degree of inflammatory infiltration of the spinal cord, as well as the lowest levels of nuclear factor kappa B, interleukin-12 and interferon-gamma.
Title: Variable effects of cyclophosphamide in rodent models of experimental allergic encephalomyelitis.

IL-12 normalizes both p38 and ERK activation in T cells, but the restoration of ERK plays a predominant role in IL-12-mediated restoration of T cell IL-2 and IFN-gamma production after EtOH and burn injury.
Title: ERK and not p38 pathway is required for IL-12 restoration of T cell IL-2 and IFN-gamma in a rodent model of alcohol intoxication and burn injury.

Measles virus infection leads to a decrease in IL-12 secretion and an increase in IL-4 secretion, but this does not correlate with immune suppression.
Title: Cytokine imbalance after measles virus infection has no correlation with immune suppression.

Data present the upregulation of IFN-gamma by Leuconostoc mesenteroides under pro-Th2 conditions and the requirement of NF-kappaB, p38 and JNK for IL-12 production.
Title: Induction of Th1 cytokines by Leuconostoc mesenteroides subsp. mesenteroides (KCTC 3100) under Th2-type conditions and the requirement of NF-kappaB and p38/JNK.

The following Il12b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Il12b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ORa06125	NM_022611.1 Latest version!	Rattus norvegicus interleukin 12B (Il12b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa32976	XM_006246147.3 Latest version!	Rattus norvegicus interleukin 12B (Il12b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Il12b ( NM_022611.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_072133.1 Rattus norvegicus interleukin 12B (Il12b), mRNA.

Price & Availability↑

CloneID	ORa06125
Clone ID Related Accession (Same CDS sequence)	NM_022611.1
Accession Version	NM_022611.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-05-20
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 subunit beta precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF133197.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF133197.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2689605, SAMN02642664 [ECO:0000348] ##Evidence-Data-END##

ATGTGTCATC AGAAGTTAAC CTTCTCCTGG TTTGCCATGG TTTTGCTGGT GTCTCCACTC 
ATGGCCATGT GGGAGCTGGA GAAAGATGTT TATGTTGTAG AGGTGGACTG GCGCCCCGAT 
GCCCCTGGAG AAACGGTGAC CCTCACCTGT GACAGTCCTG AAGAAGATGA CATCACCTGG 
ACCTCAGACC AGAGACGTGG AGTCATAGGC TCTGGAAAGA CCCTGACCAT CACTGTCAGA 
GAGTTTCTAG ATGCTGGCCA ATACACCTGC CACAGAGGAG GCGAGACTCT GAGCCACTCA 
CATCTGCTGC TCCACAAGAA GGAAAATGGA ATTTGGTCCA CCGAGATTTT AAAAAATTTC 
AAAAATAAGA CTTTCCTGAA GTGTGAAGCA CCAAACTACT CCGGACGGTT CACCTGCTCA 
TGGCTGGTGC ACAGAAACAC GGACTTGAAG TTTAACATCA AGAGCAGCAG CAGTTCCCCT 
GAGTCTCGGG CGGTGACATG TGGACGAGCA TCTCTGTCTG CAGAGAAGGT CACACTGAAC 
CAAAGGGACT ACGAGAAGTA CTCAGTGGCG TGCCAGGAGG ACGTCACCTG CCCAACTGCC 
GAGGAGACCC TGCCCATTGA ACTGGTGGTG GAGGCCCAGC AGCAGAATAA ATATGAGAAC 
TACAGCACCA GCTTCTTCAT CAGGGACATC ATCAAACCGG ACCCACCCAA GAACCTGCAG 
GTGAAACCTT TGAAGAACTC TCAGGTGGAG GTCAGCTGGG AGTACCCTGA CTCCTGGAGC 
ACTCCCCATT CCTACTTCTC CCTCAAGTTC TTCGTCCGCA TCCAGCGCAA GAAAGAAAAG 
ACGAAGGAGA CAGAGGAGGA GTGTAACCAG AAAGGTGCGT TCCTCGTAGA GAAGACCTCT 
GCCGAAGTCC AATGCAAAGG GGCGAATATC TGCGTGCAAG CGCAGGACCG CTACTACAAT 
TCATCATGCA GCAAATGGAC ATGTGTACCC TGCAGGGGCC GATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_072133.1
CDS	1..1008
Translation	MCHQKLTFSWFAMVLLVSPLMAMWELEKDVYVVEVDWRPDAPGETVTLTCDSPEEDDITWTSDQRRGVIGSGKTLTITVREFLDAGQYTCHRGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVHRNTDLKFNIKSSSSSPESRAVTCGRASLSAEKVTLNQRDYEKYSVACQEDVTCPTAEETLPIELVVEAQQQNKYENYSTSFFIRDIIKPDPPKNLQVKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKTKETEEECNQKGAFLVEKTSAEVQCKGANICVQAQDRYYNSSCSKWTCVPCRGRS

Target ORF information:

RefSeq Version	NM_022611.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12B (Il12b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_022611.1

ORF Insert Sequence:

ATGTGTCATC AGAAGTTAAC CTTCTCCTGG TTTGCCATGG TTTTGCTGGT GTCTCCACTC 
ATGGCCATGT GGGAGCTGGA GAAAGATGTT TATGTTGTAG AGGTGGACTG GCGCCCCGAT 
GCCCCTGGAG AAACGGTGAC CCTCACCTGT GACAGTCCTG AAGAAGATGA CATCACCTGG 
ACCTCAGACC AGAGACGTGG AGTCATAGGC TCTGGAAAGA CCCTGACCAT CACTGTCAGA 
GAGTTTCTAG ATGCTGGCCA ATACACCTGC CACAGAGGAG GCGAGACTCT GAGCCACTCA 
CATCTGCTGC TCCACAAGAA GGAAAATGGA ATTTGGTCCA CCGAGATTTT AAAAAATTTC 
AAAAATAAGA CTTTCCTGAA GTGTGAAGCA CCAAACTACT CCGGACGGTT CACCTGCTCA 
TGGCTGGTGC ACAGAAACAC GGACTTGAAG TTTAACATCA AGAGCAGCAG CAGTTCCCCT 
GAGTCTCGGG CGGTGACATG TGGACGAGCA TCTCTGTCTG CAGAGAAGGT CACACTGAAC 
CAAAGGGACT ACGAGAAGTA CTCAGTGGCG TGCCAGGAGG ACGTCACCTG CCCAACTGCC 
GAGGAGACCC TGCCCATTGA ACTGGTGGTG GAGGCCCAGC AGCAGAATAA ATATGAGAAC 
TACAGCACCA GCTTCTTCAT CAGGGACATC ATCAAACCGG ACCCACCCAA GAACCTGCAG 
GTGAAACCTT TGAAGAACTC TCAGGTGGAG GTCAGCTGGG AGTACCCTGA CTCCTGGAGC 
ACTCCCCATT CCTACTTCTC CCTCAAGTTC TTCGTCCGCA TCCAGCGCAA GAAAGAAAAG 
ACGAAGGAGA CAGAGGAGGA GTGTAACCAG AAAGGTGCGT TCCTCGTAGA GAAGACCTCT 
GCCGAAGTCC AATGCAAAGG GGCGAATATC TGCGTGCAAG CGCAGGACCG CTACTACAAT 
TCATCATGCA GCAAATGGAC ATGTGTACCC TGCAGGGGCC GATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Il12b ( XM_006246147.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006246209.1 Rattus norvegicus interleukin 12B (Il12b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa32976
Clone ID Related Accession (Same CDS sequence)	XM_006246147.3
Accession Version	XM_006246147.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1008bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	interleukin-12 subunit beta isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006246147.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTCATC AGAAGTTAAC CTTCTCCTGG TTTGCCATGG TTTTGCTGGT GTCTCCACTC 
ATGGCCATGT GGGAGCTGGA GAAAGATGTT TATGTTGTAG AGGTGGACTG GCGCCCCGAT 
GCCCCTGGAG AAACGGTGAC CCTCACCTGT GACAGTCCTG AAGAAGATGA CATCACCTGG 
ACCTCAGACC AGAGACGTGG AGTCATAGGC TCTGGAAAGA CCCTGACCAT CACTGTCAGA 
GAGTTTCTAG ATGCTGGCCA ATACACCTGC CACAGAGGAG GCGAGACTCT GAGCCACTCA 
CATCTGCTGC TCCACAAGAA GGAAAATGGA ATTTGGTCCA CCGAGATTTT AAAAAATTTC 
AAAAATAAGA CTTTCCTGAA GTGTGAAGCA CCAAACTACT CCGGACGGTT CACCTGCTCA 
TGGCTGGTGC ACAGAAACAC GGACTTGAAG TTTAACATCA AGAGCAGCAG CAGTTCCCCT 
GAGTCTCGGG CGGTGACATG TGGAGCAGCA TCTCTGTCTG CAGAGAAGGT CACACTGAAC 
CAAAGGGACT ACGAGAAGTA CTCAGTGGCG TGCCAGGAGG ACGTCACCTG CCCAACTGCC 
GAGGAGACCC TGCCCATTGA ACTGGTGGTG GAGGCCCAGC AGCAGAATAA ATATGAGAAC 
TACAGCACCA GCTTCTTCAT CAGGGACATC ATCAAACCGG ACCCACCCAA GAACCTGCAG 
GTGAAACCTT TGAAGAACTC TCAGGTGGAG GTCAGCTGGG AGTACCCTGA CTCCTGGAGC 
ACTCCCCATT CCTACTTCTC CCTCAAGTTC TTCGTCCGCA TCCAGCGCAA GAAAGAAAAG 
ACGAAGGAGA CAGAGGAGGA GTGTAACCAG AAAGGTGCGT TCCTCGTAGA GAAGACCTCT 
GCCGAAGTCC AATGCAAAGG GGCGAATATC TGTGTGCAAG CGCAGGACCG CTACTACAAT 
TCATCATGCA GCAAATGGAC ATGTGTACCC TGCAGGGGCC GATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006246209.1
CDS	216..1223
Translation	MCHQKLTFSWFAMVLLVSPLMAMWELEKDVYVVEVDWRPDAPGETVTLTCDSPEEDDITWTSDQRRGVIGSGKTLTITVREFLDAGQYTCHRGGETLSHSHLLLHKKENGIWSTEILKNFKNKTFLKCEAPNYSGRFTCSWLVHRNTDLKFNIKSSSSSPESRAVTCGAASLSAEKVTLNQRDYEKYSVACQEDVTCPTAEETLPIELVVEAQQQNKYENYSTSFFIRDIIKPDPPKNLQVKPLKNSQVEVSWEYPDSWSTPHSYFSLKFFVRIQRKKEKTKETEEECNQKGAFLVEKTSAEVQCKGANICVQAQDRYYNSSCSKWTCVPCRGRS

Target ORF information:

RefSeq Version	XM_006246147.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus interleukin 12B (Il12b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006246147.3

ORF Insert Sequence:

ATGTGTCATC AGAAGTTAAC CTTCTCCTGG TTTGCCATGG TTTTGCTGGT GTCTCCACTC 
ATGGCCATGT GGGAGCTGGA GAAAGATGTT TATGTTGTAG AGGTGGACTG GCGCCCCGAT 
GCCCCTGGAG AAACGGTGAC CCTCACCTGT GACAGTCCTG AAGAAGATGA CATCACCTGG 
ACCTCAGACC AGAGACGTGG AGTCATAGGC TCTGGAAAGA CCCTGACCAT CACTGTCAGA 
GAGTTTCTAG ATGCTGGCCA ATACACCTGC CACAGAGGAG GCGAGACTCT GAGCCACTCA 
CATCTGCTGC TCCACAAGAA GGAAAATGGA ATTTGGTCCA CCGAGATTTT AAAAAATTTC 
AAAAATAAGA CTTTCCTGAA GTGTGAAGCA CCAAACTACT CCGGACGGTT CACCTGCTCA 
TGGCTGGTGC ACAGAAACAC GGACTTGAAG TTTAACATCA AGAGCAGCAG CAGTTCCCCT 
GAGTCTCGGG CGGTGACATG TGGAGCAGCA TCTCTGTCTG CAGAGAAGGT CACACTGAAC 
CAAAGGGACT ACGAGAAGTA CTCAGTGGCG TGCCAGGAGG ACGTCACCTG CCCAACTGCC 
GAGGAGACCC TGCCCATTGA ACTGGTGGTG GAGGCCCAGC AGCAGAATAA ATATGAGAAC 
TACAGCACCA GCTTCTTCAT CAGGGACATC ATCAAACCGG ACCCACCCAA GAACCTGCAG 
GTGAAACCTT TGAAGAACTC TCAGGTGGAG GTCAGCTGGG AGTACCCTGA CTCCTGGAGC 
ACTCCCCATT CCTACTTCTC CCTCAAGTTC TTCGTCCGCA TCCAGCGCAA GAAAGAAAAG 
ACGAAGGAGA CAGAGGAGGA GTGTAACCAG AAAGGTGCGT TCCTCGTAGA GAAGACCTCT 
GCCGAAGTCC AATGCAAAGG GGCGAATATC TGTGTGCAAG CGCAGGACCG CTACTACAAT 
TCATCATGCA GCAAATGGAC ATGTGTACCC TGCAGGGGCC GATCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A molecular threshold for effector CD8(+) T cell differentiation controlled by transcription factors Blimp-1 and T-bet.
Nature immunology17(4)422-32(2016 Apr)
Xin A,Masson F,Liao Y,Preston S,Guan T,Gloury R,Olshansky M,Lin JX,Li P,Speed TP,Smyth GK,Ernst M,Leonard WJ,Pellegrini M,Kaech SM,Nutt SL,Shi W,Belz GT,Kallies A

Interaction of TLR-IFN and HLA polymorphisms on susceptibility of chronic HBV infection in Southwest Han Chinese.
Liver international : official journal of the International Association for the Study of the Liver35(8)1941-9(2015 Aug)
He D,Tao S,Guo S,Li M,Wu J,Huang H,Guo X,Yan G,Zhu P,Wang Y

Pellino3 ubiquitinates RIP2 and mediates Nod2-induced signaling and protective effects in colitis.
Nature immunology14(9)927-36(2013 Sep)
Yang S,Wang B,Humphries F,Jackson R,Healy ME,Bergin R,Aviello G,Hall B,McNamara D,Darby T,Quinlan A,Shanahan F,Melgar S,Fallon PG,Moynagh PN

Glucocorticoids target suppressor of cytokine signaling 1 (SOCS1) and type 1 interferons to regulate Toll-like receptor-induced STAT1 activation.
Proceedings of the National Academy of Sciences of the United States of America108(23)9554-9(2011 Jun)
Bhattacharyya S,Zhao Y,Kay TW,Muglia LJ

H-ras and N-ras are dispensable for T-cell development and activation but critical for protective Th1 immunity.
Blood117(19)5102-11(2011 May)
Iborra S,Soto M,Stark-Aroeira L,Castellano E,Alarc?n B,Alonso C,Santos E,Fern?ndez-Malav? E

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Il12b cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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