KCNT1 gene cDNA ORF clone, Bubalus bubalis(water buffalo)

mRNA	Protein	Name
XM_025261968.1	XP_025117753.1	potassium channel subfamily T member 1 isoform X1
XM_006062187.1	XP_006062249.1	potassium channel subfamily T member 1 isoform X2
XM_025261971.1	XP_025117756.1	potassium channel subfamily T member 1 isoform X3
XM_006062187.2	XP_006062249.1	potassium channel subfamily T member 1 isoform X11
XM_025261979.1	XP_025117764.1	potassium channel subfamily T member 1 isoform X12
XM_025261967.1	XP_025117752.1	potassium channel subfamily T member 1 isoform X1
XM_025261972.1	XP_025117757.1	potassium channel subfamily T member 1 isoform X4
XM_025261973.1	XP_025117758.1	potassium channel subfamily T member 1 isoform X5
XM_025261975.1	XP_025117760.1	potassium channel subfamily T member 1 isoform X7
XM_025261976.1	XP_025117761.1	potassium channel subfamily T member 1 isoform X8
XM_025261977.1	XP_025117762.1	potassium channel subfamily T member 1 isoform X9
XM_006062186.1	XP_006062248.1	potassium channel subfamily T member 1 isoform X1
XM_025261974.1	XP_025117759.1	potassium channel subfamily T member 1 isoform X6
XM_025261978.1	XP_025117763.1	potassium channel subfamily T member 1 isoform X10
XM_025261980.1	XP_025117765.1	potassium channel subfamily T member 1 isoform X13
XM_006062188.1	XP_006062250.1	potassium channel subfamily T member 1 isoform X3
XM_006062189.1	XP_006062251.1	potassium channel subfamily T member 1 isoform X4
XM_025261970.1	XP_025117755.1	potassium channel subfamily T member 1 isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBu18306	XM_006062187.2 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu18307	XM_006062188.1 Latest version!	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OBu18308	XM_006062189.1 Latest version!	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OBu46819	XM_025261967.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46819	XM_025261968.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46820	XM_025261970.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46821	XM_025261971.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46822	XM_025261972.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46823	XM_025261973.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46824	XM_025261974.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46825	XM_025261975.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46826	XM_025261976.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46827	XM_025261977.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46828	XM_025261978.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46829	XM_025261979.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu46830	XM_025261980.1 Latest version!	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBu18305	XM_006062186.1 Latest version!	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00
View Old Accession Versions >>
OBu18306	XM_006062187.1	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
Hide Old Accession Versions >>

KCNT1 ( XM_006062187.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062249.1 Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBu18306
Clone ID Related Accession (Same CDS sequence)	XM_006062187.1 , XM_006062187.2
Accession Version	XM_006062187.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785044.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTATTCTTCA TCAAAAACCA AAGATCCAGC 
CTGAGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC 
CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATTGGATGCT GGGGCTGCCC GAAGCAGAAT 
TACACCTTCA ATGAGTCGTC CTCTGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG TGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGCGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCCTGCT GTGCCTGGTC TTCACGGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACGTCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACACCC AAGATCTGGC CATCCCAGCT GCTGGTGGTC 
ATCATGATCT GCGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGGTT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA CGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CGGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCGAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AACAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCCGTG CACAGCATCA TCGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAACAC GGAGTGCCGG CCCACACAGA GTGGCGGGGG CAGCGGGGGC 
AGCAAGCTGG CGCTCCCCAC AGAGAACGGC TCGGGCAGCC GGCGGCCCAG CATCGCGCCC 
GTGCTGGAGG TGGCTGACAG CTCGGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGACGAG GGGCTGTCCA TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCCACCC TGTGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ACGGCTTCAA GAACAAGCTG ATCATTGTCT CGGCGGAGAC CGCGGGCAAT 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGCGTCATCT ATGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGTGCCGAG 
GAGGACTACA TGGCCGATGC AAAGACCATC GTCAACGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCTGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAC GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAATGCC ACGTCTTCTC CACCGAGCCC 
CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062249.1
CDS	121..3696
Translation	MSRPEWSLECGYRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006062187.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062187.1

ORF Insert Sequence:

ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTATTCTTCA TCAAAAACCA AAGATCCAGC 
CTGAGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC 
CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATTGGATGCT GGGGCTGCCC GAAGCAGAAT 
TACACCTTCA ATGAGTCGTC CTCTGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG TGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGCGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCCTGCT GTGCCTGGTC TTCACGGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACGTCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACACCC AAGATCTGGC CATCCCAGCT GCTGGTGGTC 
ATCATGATCT GCGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGGTT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA CGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CGGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCGAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AACAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCCGTG CACAGCATCA TCGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAACAC GGAGTGCCGG CCCACACAGA GTGGCGGGGG CAGCGGGGGC 
AGCAAGCTGG CGCTCCCCAC AGAGAACGGC TCGGGCAGCC GGCGGCCCAG CATCGCGCCC 
GTGCTGGAGG TGGCTGACAG CTCGGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGACGAG GGGCTGTCCA TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCCACCC TGTGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ACGGCTTCAA GAACAAGCTG ATCATTGTCT CGGCGGAGAC CGCGGGCAAT 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGCGTCATCT ATGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGTGCCGAG 
GAGGACTACA TGGCCGATGC AAAGACCATC GTCAACGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCTGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAC GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAATGCC ACGTCTTCTC CACCGAGCCC 
CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_006062187.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062249.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA.

Price & Availability↑

CloneID	OBu18306
Clone ID Related Accession (Same CDS sequence)	XM_006062187.1 , XM_006062187.2
Accession Version	XM_006062187.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3576bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006062187.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTATTCTTCA TCAAAAACCA AAGATCCAGC 
CTGAGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC 
CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATTGGATGCT GGGGCTGCCC GAAGCAGAAT 
TACACCTTCA ATGAGTCGTC CTCTGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG TGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGCGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCCTGCT GTGCCTGGTC TTCACGGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACGTCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACACCC AAGATCTGGC CATCCCAGCT GCTGGTGGTC 
ATCATGATCT GCGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGGTT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA CGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CGGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCGAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AACAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCCGTG CACAGCATCA TCGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAACAC GGAGTGCCGG CCCACACAGA GTGGCGGGGG CAGCGGGGGC 
AGCAAGCTGG CGCTCCCCAC AGAGAACGGC TCGGGCAGCC GGCGGCCCAG CATCGCGCCC 
GTGCTGGAGG TGGCTGACAG CTCGGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGACGAG GGGCTGTCCA TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCCACCC TGTGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ACGGCTTCAA GAACAAGCTG ATCATTGTCT CGGCGGAGAC CGCGGGCAAT 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGCGTCATCT ATGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGTGCCGAG 
GAGGACTACA TGGCCGATGC AAAGACCATC GTCAACGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCTGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAC GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAATGCC ACGTCTTCTC CACCGAGCCC 
CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062249.1
CDS	173..3748
Translation	MSRPEWSLECGYRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006062187.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062187.2

ORF Insert Sequence:

ATGTCCCGCC CCGAATGGAG CCTCGAGTGC GGGTACAGGG TCCAGGTGGA GTTCTACGTC 
AACGAGAACA CCTTCAAGGA GAGGCTTAAG CTATTCTTCA TCAAAAACCA AAGATCCAGC 
CTGAGGATCC GGCTGTTCAA CTTCTCCCTG AAGCTGCTGA CCTGCCTGCT GTACATTGTC 
CGCGTCCTGC TGGACGACCC AGCCCTGGGG ATTGGATGCT GGGGCTGCCC GAAGCAGAAT 
TACACCTTCA ATGAGTCGTC CTCTGAGATC AACTGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG TGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGCGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCCTGCT GTGCCTGGTC TTCACGGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACGTCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACACCC AAGATCTGGC CATCCCAGCT GCTGGTGGTC 
ATCATGATCT GCGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGGTT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA CGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CGGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCGAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AACAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCCGTG CACAGCATCA TCGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAACAC GGAGTGCCGG CCCACACAGA GTGGCGGGGG CAGCGGGGGC 
AGCAAGCTGG CGCTCCCCAC AGAGAACGGC TCGGGCAGCC GGCGGCCCAG CATCGCGCCC 
GTGCTGGAGG TGGCTGACAG CTCGGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGACGAG GGGCTGTCCA TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCCACCC TGTGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ACGGCTTCAA GAACAAGCTG ATCATTGTCT CGGCGGAGAC CGCGGGCAAT 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGCGTCATCT ATGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGTGCCGAG 
GAGGACTACA TGGCCGATGC AAAGACCATC GTCAACGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCTGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAC GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAATGCC ACGTCTTCTC CACCGAGCCC 
CCCGACCTCA GGGCCCAGGC CTCACCCCTC CTCCCCGGCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_006062188.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062250.1 Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

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CloneID	OBu18307
Clone ID Related Accession (Same CDS sequence)	XM_006062188.1
Accession Version	XM_006062188.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3636bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785044.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTTC CCGACAGGGT GCACAGGGGT CCAGGAGATG TCTACAGAGG GGCACAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGGTCCAG 
GTGGAGTTCT ACGTCAACGA GAACACCTTC AAGGAGAGGC TTAAGCTATT CTTCATCAAA 
AACCAAAGAT CCAGCCTGAG GATCCGGCTG TTCAACTTCT CCCTGAAGCT GCTGACCTGC 
CTGCTGTACA TTGTCCGCGT CCTGCTGGAC GACCCAGCCC TGGGGATTGG ATGCTGGGGC 
TGCCCGAAGC AGAATTACAC CTTCAATGAG TCGTCCTCTG AGATCAACTG GGCCCCCATC 
CTGTGGGTGG AGAGGAAGGT GGCTCTGTGG GCCATTCAGG TCATCGTGGC CATCATCAGC 
TTCCTGGAGA CCATGCTTCT CATTTACCTC AGCTACAAAG GCAACATCTG GGAGCAGATC 
TTCCGTGTGT CCTTCATCCT GGAGATGATC AACACGCTGC CCTTCATCAT CACGATATTC 
TGGCCGCCGC TGCGGAACCT GTTCATCCCC GTGTTTCTGA ACTGCTGGCT GGCCAAGCAC 
GCCCTGGAGA ACATGATCAA CGACTTCCAC CGCGCCATCC TGCGCTCGCA GTCGGCCATG 
TTCAATCAGG TCCTCATCCT CTTCTGCACC CTGCTGTGCC TGGTCTTCAC GGGGGGCTGT 
GTGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062250.1
CDS	1..3636
Translation	MPLPDRVHRGPGDVYRGAQGGGHTNRTFVFDDGQCAPRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGGCVTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006062188.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062188.1

ORF Insert Sequence:

ATGCCGCTTC CCGACAGGGT GCACAGGGGT CCAGGAGATG TCTACAGAGG GGCACAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGGTCCAG 
GTGGAGTTCT ACGTCAACGA GAACACCTTC AAGGAGAGGC TTAAGCTATT CTTCATCAAA 
AACCAAAGAT CCAGCCTGAG GATCCGGCTG TTCAACTTCT CCCTGAAGCT GCTGACCTGC 
CTGCTGTACA TTGTCCGCGT CCTGCTGGAC GACCCAGCCC TGGGGATTGG ATGCTGGGGC 
TGCCCGAAGC AGAATTACAC CTTCAATGAG TCGTCCTCTG AGATCAACTG GGCCCCCATC 
CTGTGGGTGG AGAGGAAGGT GGCTCTGTGG GCCATTCAGG TCATCGTGGC CATCATCAGC 
TTCCTGGAGA CCATGCTTCT CATTTACCTC AGCTACAAAG GCAACATCTG GGAGCAGATC 
TTCCGTGTGT CCTTCATCCT GGAGATGATC AACACGCTGC CCTTCATCAT CACGATATTC 
TGGCCGCCGC TGCGGAACCT GTTCATCCCC GTGTTTCTGA ACTGCTGGCT GGCCAAGCAC 
GCCCTGGAGA ACATGATCAA CGACTTCCAC CGCGCCATCC TGCGCTCGCA GTCGGCCATG 
TTCAATCAGG TCCTCATCCT CTTCTGCACC CTGCTGTGCC TGGTCTTCAC GGGGGGCTGT 
GTGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_006062189.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062251.1 Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

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CloneID	OBu18308
Clone ID Related Accession (Same CDS sequence)	XM_006062189.1
Accession Version	XM_006062189.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3468bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785044.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTA TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATT GGATGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG TGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGCGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCCTGCT GTGCCTGGTC TTCACGGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACGTCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACACCC AAGATCTGGC CATCCCAGCT GCTGGTGGTC 
ATCATGATCT GCGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGGTT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA CGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CGGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCGAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AACAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCCGTG CACAGCATCA TCGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAACAC GGAGTGCCGG CCCACACAGA GTGGCGGGGG CAGCGGGGGC 
AGCAAGCTGG CGCTCCCCAC AGAGAACGGC TCGGGCAGCC GGCGGCCCAG CATCGCGCCC 
GTGCTGGAGG TGGCTGACAG CTCGGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGACGAG GGGCTGTCCA TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCCACCC TGTGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ACGGCTTCAA GAACAAGCTG ATCATTGTCT CGGCGGAGAC CGCGGGCAAT 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGCGTCATCT ATGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGTGCCGAG 
GAGGACTACA TGGCCGATGC AAAGACCATC GTCAACGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCTGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAC GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAATGCC ACGTCTTCTC CACCGAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCATGT GGCCAGCAGC TCCCAGAGCA GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCTT CCTGCAACCC CGAGACGCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062251.1
CDS	27..3494
Translation	MSDLDSEVLPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTESQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006062189.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062189.1

ORF Insert Sequence:

ATGAGCGACC TGGACTCGGA GGTGCTGCCC TTGCCTCCGC GGTACCGCTT CCGGGACCTG 
CTGCTGGGCG ACCAGTCCTT CCAGAACGAC GACAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTA TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATT GGATGGGCCC CCATCCTGTG GGTGGAGAGG 
AAGGTGGCTC TGTGGGCCAT TCAGGTCATC GTGGCCATCA TCAGCTTCCT GGAGACCATG 
CTTCTCATTT ACCTCAGCTA CAAAGGCAAC ATCTGGGAGC AGATCTTCCG TGTGTCCTTC 
ATCCTGGAGA TGATCAACAC GCTGCCCTTC ATCATCACGA TATTCTGGCC GCCGCTGCGG 
AACCTGTTCA TCCCCGTGTT TCTGAACTGC TGGCTGGCCA AGCACGCCCT GGAGAACATG 
ATCAACGACT TCCACCGCGC CATCCTGCGC TCGCAGTCGG CCATGTTCAA TCAGGTCCTC 
ATCCTCTTCT GCACCCTGCT GTGCCTGGTC TTCACGGGGA CCTGCGGCAT CCAGCACCTG 
GAGCGCGCGG GCGACAACCT GTCCCTCCTG ACGTCCTTCT ACTTCTGCAT CGTCACCTTC 
TCCACCGTGG GCTATGGGGA CGTGACACCC AAGATCTGGC CATCCCAGCT GCTGGTGGTC 
ATCATGATCT GCGTGGCCCT GGTGGTGCTC CCGCTGCAGT TTGAGGAACT CGTCTACCTG 
TGGATGGAGC GGCAGAAGTC GGGGGGCAAC TACAGCCGCC ACCGGGCCCA GACGGAGAAG 
CATGTGATCC TGTGCGTGAG CTCCCTCAAG ATCGACCTGC TCATGGACTT TCTGAACGAG 
TTCTACGCGC ATCCACGGTT GCAGGACTAC TACGTCGTCA TCCTGTGCCC CACGGAGATG 
GACATCCAGG TTCGCAGGGT CCTGCAGATC CCACTGTGGT CCCAGCGGGT CATCTACCTC 
CAGGGCTCCG CGCTCAAGGA TCAGGACCTC ATGCGGGCCA AGATGGACAA CGGGGAGGCC 
TGCTTTATTC TCAGCAGCCG GAACGAGGTG GACCGCACCG CGGCGGACCA CCAGACCATA 
CTGCGTGCCT GGGCCGTGAA GGACTTTGCC CCGAACTGCC CCCTCTACGT CCAGATCCTC 
AAGCCTGAGA ACAAGTTTCA TGTCAAGTTT GCAGAGCACG TGGTGTGCGA GGAGGAGTGC 
AAGTATGCCA TGCTGGCCCT CAACTGCATC TGCCCAGCCA CGTCCACCCT CATCACCCTG 
CTGGTGCACA CGTCCCGCGG CCAGGAAGGC CAGGACTCGC CAGAGCAGTG GCAGCGCATG 
TACGGACGCT GCTCGGGCAA CGAGGTCTAC CACGTGCGGA TGGGGGACAG CAAGTTCTTC 
CGCGAGTACG AGGGCAAGAG CTTCACCTAC GCCGCCTTCC ACGCGCACAA GAAGTACGGC 
GTGTGCCTCA TCGGCCTGAA GCGCGAGGAC AACAAGAGCA TCCTGCTGAA CCCGGGCCCA 
CGGCACATCC TGGCGGCCTC CGACACCTGC TTCTACATCA ACATCACCAA GGAGGAGAAC 
TCGGCCTTCA TCTTCAAGCA GGAGGAAAAG CAGAAGAGGA AGGGCTTCGC GGGGCAGGGG 
CTGTACGAGG GCTCATCCCG CCTGCCCGTG CACAGCATCA TCGCCTCCAT GGGGACAGTG 
GCCATGGACC TCCAGAACAC GGAGTGCCGG CCCACACAGA GTGGCGGGGG CAGCGGGGGC 
AGCAAGCTGG CGCTCCCCAC AGAGAACGGC TCGGGCAGCC GGCGGCCCAG CATCGCGCCC 
GTGCTGGAGG TGGCTGACAG CTCGGCCCTG CTGCCCTGCG ACCTGCTGAG TGACCAGTCA 
GAGGACGAGA TGACGCCGTC GGAGGACGAG GGGCTGTCCA TGGTGGAGTA TGTGAAGGGC 
TACCCTCCTA ACTCACCCTA CATCGGCAGC TCCCCCACCC TGTGCCACCT CCTGCCTGTG 
AAAGCCCCCT TCTGCTGCCT GCGGCTGGAC AAGGGCTGCA AGCACAACAG CTACGAAGAT 
GCCAAGGCCT ACGGCTTCAA GAACAAGCTG ATCATTGTCT CGGCGGAGAC CGCGGGCAAT 
GGGCTGTACA ACTTCATCGT GCCGCTGCGC GCCTACTACC GGCCTCGCCG GGAGCTCAAC 
CCCATTGTGC TGCTGCTGGA CAACAAGCCC GACCACCACT TCCTGGAGGC CATCTGCTGC 
TTTCCCATGG TCTACTACAT GGAGGGCTCC GTGGACAACC TGGACAGCCT GCTGCGGTGC 
GGCGTCATCT ATGCGGACAA TCTGGTGGTG GCAGACAAGG AGAGCACCAT GAGTGCCGAG 
GAGGACTACA TGGCCGATGC AAAGACCATC GTCAACGTGC AGACCATGTT CCGGCTCTTC 
CCCAGTCTCA GCATCACCAC CGAGCTCACC CACCCGTCCA ACATGCGGTT CATGCAGTTT 
CGCGCCAAGG ACAGCTACTC TCTGGCTCTC TCTAAACTGG AGAAGAGGGA GCGTGAGAAC 
GGCTCCAACC TGGCCTTCAT GTTCCGCCTG CCCTTCGCCG CCGGCCGCGT CTTCAGCATC 
AGCATGCTGG ACACACTGCT GTATCAGTCC TTCGTGAAGG ACTACATGAT CCCCATCACC 
AGGCTGTTGC TGGGCTTGGA CACCACGCCG GGCTCTGGCT ACCTCTGTGC GATGAAGATC 
TCGGAGGACG ACCTGTGGAT CCGCACCTAC GGCCGCCTCT TCCAGAAGCT GTGCTCCTCC 
AGCGCCGAGA TCCCTATCGG CATCTACAGG ACCCAATGCC ACGTCTTCTC CACCGAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCATGT GGCCAGCAGC TCCCAGAGCA GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCTT CCTGCAACCC CGAGACGCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261968.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117753.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA.

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CloneID	OBu46819
Clone ID Related Accession (Same CDS sequence)	XM_025261968.1 , XM_025261967.1
Accession Version	XM_025261968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAT GTGGCCAGCA GCTCCCAGAG CAGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC TTCCTGCAAC CCCGAGACGC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117753.1
CDS	16..3795
Translation	MYPHCHGCHAASGASGGQAPPRGVRMGVPCSPRPLPESSLCVREPRLPHPSIRPLSALMCPLHSTLRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261968.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261968.1

ORF Insert Sequence:

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAT GTGGCCAGCA GCTCCCAGAG CAGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC TTCCTGCAAC CCCGAGACGC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261967.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117752.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA.

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CloneID	OBu46819
Clone ID Related Accession (Same CDS sequence)	XM_025261968.1 , XM_025261967.1
Accession Version	XM_025261967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3780bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAT GTGGCCAGCA GCTCCCAGAG CAGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC TTCCTGCAAC CCCGAGACGC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117752.1
CDS	16..3795
Translation	MYPHCHGCHAASGASGGQAPPRGVRMGVPCSPRPLPESSLCVREPRLPHPSIRPLSALMCPLHSTLRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261967.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261967.1

ORF Insert Sequence:

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAT GTGGCCAGCA GCTCCCAGAG CAGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC TTCCTGCAAC CCCGAGACGC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261970.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117755.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBu46820
Clone ID Related Accession (Same CDS sequence)	XM_025261970.1
Accession Version	XM_025261970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3774bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGTGGC CATGGACCTC CAGAACACGG AGTGCCGGCC CACACAGAGT 
GGCGGGGGCA GCGGGGGCAG CAAGCTGGCG CTCCCCACAG AGAACGGCTC GGGCAGCCGG 
CGGCCCAGCA TCGCGCCCGT GCTGGAGGTG GCTGACAGCT CGGCCCTGCT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGACGAGATG ACGCCGTCGG AGGACGAGGG GCTGTCCATG 
GTGGAGTATG TGAAGGGCTA CCCTCCTAAC TCACCCTACA TCGGCAGCTC CCCCACCCTG 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGCTGGACAA GGGCTGCAAG 
CACAACAGCT ACGAAGATGC CAAGGCCTAC GGCTTCAAGA ACAAGCTGAT CATTGTCTCG 
GCGGAGACCG CGGGCAATGG GCTGTACAAC TTCATCGTGC CGCTGCGCGC CTACTACCGG 
CCTCGCCGGG AGCTCAACCC CATTGTGCTG CTGCTGGACA ACAAGCCCGA CCACCACTTC 
CTGGAGGCCA TCTGCTGCTT TCCCATGGTC TACTACATGG AGGGCTCCGT GGACAACCTG 
GACAGCCTGC TGCGGTGCGG CGTCATCTAT GCGGACAATC TGGTGGTGGC AGACAAGGAG 
AGCACCATGA GTGCCGAGGA GGACTACATG GCCGATGCAA AGACCATCGT CAACGTGCAG 
ACCATGTTCC GGCTCTTCCC CAGTCTCAGC ATCACCACCG AGCTCACCCA CCCGTCCAAC 
ATGCGGTTCA TGCAGTTTCG CGCCAAGGAC AGCTACTCTC TGGCTCTCTC TAAACTGGAG 
AAGAGGGAGC GTGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC CTTCGCCGCC 
GGCCGCGTCT TCAGCATCAG CATGCTGGAC ACACTGCTGT ATCAGTCCTT CGTGAAGGAC 
TACATGATCC CCATCACCAG GCTGTTGCTG GGCTTGGACA CCACGCCGGG CTCTGGCTAC 
CTCTGTGCGA TGAAGATCTC GGAGGACGAC CTGTGGATCC GCACCTACGG CCGCCTCTTC 
CAGAAGCTGT GCTCCTCCAG CGCCGAGATC CCTATCGGCA TCTACAGGAC CCAATGCCAC 
GTCTTCTCCA CCGAGCCCCC CGACCTCAGG GCCCAGGCCT CACCCCTCCT CCCCGGCCAG 
TCCCAGGTCT CAGTGAGCGT GGAGGACTGT GAGGACACTC GGGAGGCAAA GGGGCCCTGG 
GGTGCACGGG TGGGCACGAG CGGTGGCAGC ACCCACGGCC GGCACACGGT GGGCAGCGAC 
CCAGCCGAGC ACCCGCTCCT GCGGCGCAAG AGCCTCCCGT GGGCGCGGAG GCTGAGCCGC 
AAGGGCTCCC GGCACACGGG GAAAGCGGCA GCCGAGTGGA TCAGCCAACA GCGGCTCAGC 
CTGTACCGGC GCTCGGAGCG GCAGGAGCTC TCAGAGCTGG TCAAGAACCG CATGAAGCAC 
CTGGGGCTGC CCACCACTGG CTACGAGGAT GTAGCAAATT TAACAGCCAG TGATGTCATG 
AATCGGGTAA ACCTGGGATA TTTGCAAGAC GAGATGAATG ACCACCAGAA CACCCTCTCC 
TATGTGCTCA TCAACCCCCC GCCCGACACG CGGCTGGAGC CCAATGACAT TGTATATCTC 
ATCCGCTCCG ACCCCTTGGC CCATGTGGCC AGCAGCTCCC AGAGCAGGAA GAGCAGCTGC 
AGCAACAAGC TGGCTTCCTG CAACCCCGAG ACGCGGGACG AGACACAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117755.1
CDS	16..3789
Translation	MYPHCHGCHAASGASGGQAPPRGVRMGVPCSPRPLPESSLCVREPRLPHPSIRPLSALMCPLHSTLRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261970.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261970.1

ORF Insert Sequence:

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGTGGC CATGGACCTC CAGAACACGG AGTGCCGGCC CACACAGAGT 
GGCGGGGGCA GCGGGGGCAG CAAGCTGGCG CTCCCCACAG AGAACGGCTC GGGCAGCCGG 
CGGCCCAGCA TCGCGCCCGT GCTGGAGGTG GCTGACAGCT CGGCCCTGCT GCCCTGCGAC 
CTGCTGAGTG ACCAGTCAGA GGACGAGATG ACGCCGTCGG AGGACGAGGG GCTGTCCATG 
GTGGAGTATG TGAAGGGCTA CCCTCCTAAC TCACCCTACA TCGGCAGCTC CCCCACCCTG 
TGCCACCTCC TGCCTGTGAA AGCCCCCTTC TGCTGCCTGC GGCTGGACAA GGGCTGCAAG 
CACAACAGCT ACGAAGATGC CAAGGCCTAC GGCTTCAAGA ACAAGCTGAT CATTGTCTCG 
GCGGAGACCG CGGGCAATGG GCTGTACAAC TTCATCGTGC CGCTGCGCGC CTACTACCGG 
CCTCGCCGGG AGCTCAACCC CATTGTGCTG CTGCTGGACA ACAAGCCCGA CCACCACTTC 
CTGGAGGCCA TCTGCTGCTT TCCCATGGTC TACTACATGG AGGGCTCCGT GGACAACCTG 
GACAGCCTGC TGCGGTGCGG CGTCATCTAT GCGGACAATC TGGTGGTGGC AGACAAGGAG 
AGCACCATGA GTGCCGAGGA GGACTACATG GCCGATGCAA AGACCATCGT CAACGTGCAG 
ACCATGTTCC GGCTCTTCCC CAGTCTCAGC ATCACCACCG AGCTCACCCA CCCGTCCAAC 
ATGCGGTTCA TGCAGTTTCG CGCCAAGGAC AGCTACTCTC TGGCTCTCTC TAAACTGGAG 
AAGAGGGAGC GTGAGAACGG CTCCAACCTG GCCTTCATGT TCCGCCTGCC CTTCGCCGCC 
GGCCGCGTCT TCAGCATCAG CATGCTGGAC ACACTGCTGT ATCAGTCCTT CGTGAAGGAC 
TACATGATCC CCATCACCAG GCTGTTGCTG GGCTTGGACA CCACGCCGGG CTCTGGCTAC 
CTCTGTGCGA TGAAGATCTC GGAGGACGAC CTGTGGATCC GCACCTACGG CCGCCTCTTC 
CAGAAGCTGT GCTCCTCCAG CGCCGAGATC CCTATCGGCA TCTACAGGAC CCAATGCCAC 
GTCTTCTCCA CCGAGCCCCC CGACCTCAGG GCCCAGGCCT CACCCCTCCT CCCCGGCCAG 
TCCCAGGTCT CAGTGAGCGT GGAGGACTGT GAGGACACTC GGGAGGCAAA GGGGCCCTGG 
GGTGCACGGG TGGGCACGAG CGGTGGCAGC ACCCACGGCC GGCACACGGT GGGCAGCGAC 
CCAGCCGAGC ACCCGCTCCT GCGGCGCAAG AGCCTCCCGT GGGCGCGGAG GCTGAGCCGC 
AAGGGCTCCC GGCACACGGG GAAAGCGGCA GCCGAGTGGA TCAGCCAACA GCGGCTCAGC 
CTGTACCGGC GCTCGGAGCG GCAGGAGCTC TCAGAGCTGG TCAAGAACCG CATGAAGCAC 
CTGGGGCTGC CCACCACTGG CTACGAGGAT GTAGCAAATT TAACAGCCAG TGATGTCATG 
AATCGGGTAA ACCTGGGATA TTTGCAAGAC GAGATGAATG ACCACCAGAA CACCCTCTCC 
TATGTGCTCA TCAACCCCCC GCCCGACACG CGGCTGGAGC CCAATGACAT TGTATATCTC 
ATCCGCTCCG ACCCCTTGGC CCATGTGGCC AGCAGCTCCC AGAGCAGGAA GAGCAGCTGC 
AGCAACAAGC TGGCTTCCTG CAACCCCGAG ACGCGGGACG AGACACAGCT CTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261971.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117756.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBu46821
Clone ID Related Accession (Same CDS sequence)	XM_025261971.1
Accession Version	XM_025261971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3756bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117756.1
CDS	16..3771
Translation	MYPHCHGCHAASGASGGQAPPRGVRMGVPCSPRPLPESSLCVREPRLPHPSIRPLSALMCPLHSTLRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261971.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261971.1

ORF Insert Sequence:

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGTCCCAGGT CTCAGTGAGC 
GTGGAGGACT GTGAGGACAC TCGGGAGGCA AAGGGGCCCT GGGGTGCACG GGTGGGCACG 
AGCGGTGGCA GCACCCACGG CCGGCACACG GTGGGCAGCG ACCCAGCCGA GCACCCGCTC 
CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG AGGCTGAGCC GCAAGGGCTC CCGGCACACG 
GGGAAAGCGG CAGCCGAGTG GATCAGCCAA CAGCGGCTCA GCCTGTACCG GCGCTCGGAG 
CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC CGCATGAAGC ACCTGGGGCT GCCCACCACT 
GGCTACGAGG ATGTAGCAAA TTTAACAGCC AGTGATGTCA TGAATCGGGT AAACCTGGGA 
TATTTGCAAG ACGAGATGAA TGACCACCAG AACACCCTCT CCTATGTGCT CATCAACCCC 
CCGCCCGACA CGCGGCTGGA GCCCAATGAC ATTGTATATC TCATCCGCTC CGACCCCTTG 
GCCCATGTGG CCAGCAGCTC CCAGAGCAGG AAGAGCAGCT GCAGCAACAA GCTGGCTTCC 
TGCAACCCCG AGACGCGGGA CGAGACACAG CTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261972.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117757.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBu46822
Clone ID Related Accession (Same CDS sequence)	XM_025261972.1
Accession Version	XM_025261972.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG GTGGCTCTGT GGGCCATTCA GGTCATCGTG 
GCCATCATCA GCTTCCTGGA GACCATGCTT CTCATTTACC TCAGCTACAA AGGCAACATC 
TGGGAGCAGA TCTTCCGTGT GTCCTTCATC CTGGAGATGA TCAACACGCT GCCCTTCATC 
ATCACGATAT TCTGGCCGCC GCTGCGGAAC CTGTTCATCC CCGTGTTTCT GAACTGCTGG 
CTGGCCAAGC ACGCCCTGGA GAACATGATC AACGACTTCC ACCGCGCCAT CCTGCGCTCG 
CAGTCGGCCA TGTTCAATCA GGTCCTCATC CTCTTCTGCA CCCTGCTGTG CCTGGTCTTC 
ACGGGGACCT GCGGCATCCA GCACCTGGAG CGCGCGGGCG ACAACCTGTC CCTCCTGACG 
TCCTTCTACT TCTGCATCGT CACCTTCTCC ACCGTGGGCT ATGGGGACGT GACACCCAAG 
ATCTGGCCAT CCCAGCTGCT GGTGGTCATC ATGATCTGCG TGGCCCTGGT GGTGCTCCCG 
CTGCAGTTTG AGGAACTCGT CTACCTGTGG ATGGAGCGGC AGAAGTCGGG GGGCAACTAC 
AGCCGCCACC GGGCCCAGAC GGAGAAGCAT GTGATCCTGT GCGTGAGCTC CCTCAAGATC 
GACCTGCTCA TGGACTTTCT GAACGAGTTC TACGCGCATC CACGGTTGCA GGACTACTAC 
GTCGTCATCC TGTGCCCCAC GGAGATGGAC ATCCAGGTTC GCAGGGTCCT GCAGATCCCA 
CTGTGGTCCC AGCGGGTCAT CTACCTCCAG GGCTCCGCGC TCAAGGATCA GGACCTCATG 
CGGGCCAAGA TGGACAACGG GGAGGCCTGC TTTATTCTCA GCAGCCGGAA CGAGGTGGAC 
CGCACCGCGG CGGACCACCA GACCATACTG CGTGCCTGGG CCGTGAAGGA CTTTGCCCCG 
AACTGCCCCC TCTACGTCCA GATCCTCAAG CCTGAGAACA AGTTTCATGT CAAGTTTGCA 
GAGCACGTGG TGTGCGAGGA GGAGTGCAAG TATGCCATGC TGGCCCTCAA CTGCATCTGC 
CCAGCCACGT CCACCCTCAT CACCCTGCTG GTGCACACGT CCCGCGGCCA GGAAGGCCAG 
GACTCGCCAG AGCAGTGGCA GCGCATGTAC GGACGCTGCT CGGGCAACGA GGTCTACCAC 
GTGCGGATGG GGGACAGCAA GTTCTTCCGC GAGTACGAGG GCAAGAGCTT CACCTACGCC 
GCCTTCCACG CGCACAAGAA GTACGGCGTG TGCCTCATCG GCCTGAAGCG CGAGGACAAC 
AAGAGCATCC TGCTGAACCC GGGCCCACGG CACATCCTGG CGGCCTCCGA CACCTGCTTC 
TACATCAACA TCACCAAGGA GGAGAACTCG GCCTTCATCT TCAAGCAGGA GGAAAAGCAG 
AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG TACGAGGGCT CATCCCGCCT GCCCGTGCAC 
AGCATCATCG CCTCCATGGG GACAGTGGCC ATGGACCTCC AGAACACGGA GTGCCGGCCC 
ACACAGAGTG GCGGGGGCAG CGGGGGCAGC AAGCTGGCGC TCCCCACAGA GAACGGCTCG 
GGCAGCCGGC GGCCCAGCAT CGCGCCCGTG CTGGAGGTGG CTGACAGCTC GGCCCTGCTG 
CCCTGCGACC TGCTGAGTGA CCAGTCAGAG GACGAGATGA CGCCGTCGGA GGACGAGGGG 
CTGTCCATGG TGGAGTATGT GAAGGGCTAC CCTCCTAACT CACCCTACAT CGGCAGCTCC 
CCCACCCTGT GCCACCTCCT GCCTGTGAAA GCCCCCTTCT GCTGCCTGCG GCTGGACAAG 
GGCTGCAAGC ACAACAGCTA CGAAGATGCC AAGGCCTACG GCTTCAAGAA CAAGCTGATC 
ATTGTCTCGG CGGAGACCGC GGGCAATGGG CTGTACAACT TCATCGTGCC GCTGCGCGCC 
TACTACCGGC CTCGCCGGGA GCTCAACCCC ATTGTGCTGC TGCTGGACAA CAAGCCCGAC 
CACCACTTCC TGGAGGCCAT CTGCTGCTTT CCCATGGTCT ACTACATGGA GGGCTCCGTG 
GACAACCTGG ACAGCCTGCT GCGGTGCGGC GTCATCTATG CGGACAATCT GGTGGTGGCA 
GACAAGGAGA GCACCATGAG TGCCGAGGAG GACTACATGG CCGATGCAAA GACCATCGTC 
AACGTGCAGA CCATGTTCCG GCTCTTCCCC AGTCTCAGCA TCACCACCGA GCTCACCCAC 
CCGTCCAACA TGCGGTTCAT GCAGTTTCGC GCCAAGGACA GCTACTCTCT GGCTCTCTCT 
AAACTGGAGA AGAGGGAGCG TGAGAACGGC TCCAACCTGG CCTTCATGTT CCGCCTGCCC 
TTCGCCGCCG GCCGCGTCTT CAGCATCAGC ATGCTGGACA CACTGCTGTA TCAGTCCTTC 
GTGAAGGACT ACATGATCCC CATCACCAGG CTGTTGCTGG GCTTGGACAC CACGCCGGGC 
TCTGGCTACC TCTGTGCGAT GAAGATCTCG GAGGACGACC TGTGGATCCG CACCTACGGC 
CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC GCCGAGATCC CTATCGGCAT CTACAGGACC 
CAATGCCACG TCTTCTCCAC CGAGCCCCCC GACCTCAGGG CCCAGGCCTC ACCCCTCCTC 
CCCGGCCAGT CCCAGGTCTC AGTGAGCGTG GAGGACTGTG AGGACACTCG GGAGGCAAAG 
GGGCCCTGGG GTGCACGGGT GGGCACGAGC GGTGGCAGCA CCCACGGCCG GCACACGGTG 
GGCAGCGACC CAGCCGAGCA CCCGCTCCTG CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG 
CTGAGCCGCA AGGGCTCCCG GCACACGGGG AAAGCGGCAG CCGAGTGGAT CAGCCAACAG 
CGGCTCAGCC TGTACCGGCG CTCGGAGCGG CAGGAGCTCT CAGAGCTGGT CAAGAACCGC 
ATGAAGCACC TGGGGCTGCC CACCACTGGC TACGAGGATG TAGCAAATTT AACAGCCAGT 
GATGTCATGA ATCGGGTAAA CCTGGGATAT TTGCAAGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CATGTGGCCA GCAGCTCCCA GAGCAGGAAG 
AGCAGCTGCA GCAACAAGCT GGCTTCCTGC AACCCCGAGA CGCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117757.1
CDS	16..3738
Translation	MYPHCHGCHAASGASGGQAPPRGVRMGVPCSPRPLPESSLCVREPRLPHPSIRPLSALMCPLHSTLRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261972.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261972.1

ORF Insert Sequence:

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG GTGGCTCTGT GGGCCATTCA GGTCATCGTG 
GCCATCATCA GCTTCCTGGA GACCATGCTT CTCATTTACC TCAGCTACAA AGGCAACATC 
TGGGAGCAGA TCTTCCGTGT GTCCTTCATC CTGGAGATGA TCAACACGCT GCCCTTCATC 
ATCACGATAT TCTGGCCGCC GCTGCGGAAC CTGTTCATCC CCGTGTTTCT GAACTGCTGG 
CTGGCCAAGC ACGCCCTGGA GAACATGATC AACGACTTCC ACCGCGCCAT CCTGCGCTCG 
CAGTCGGCCA TGTTCAATCA GGTCCTCATC CTCTTCTGCA CCCTGCTGTG CCTGGTCTTC 
ACGGGGACCT GCGGCATCCA GCACCTGGAG CGCGCGGGCG ACAACCTGTC CCTCCTGACG 
TCCTTCTACT TCTGCATCGT CACCTTCTCC ACCGTGGGCT ATGGGGACGT GACACCCAAG 
ATCTGGCCAT CCCAGCTGCT GGTGGTCATC ATGATCTGCG TGGCCCTGGT GGTGCTCCCG 
CTGCAGTTTG AGGAACTCGT CTACCTGTGG ATGGAGCGGC AGAAGTCGGG GGGCAACTAC 
AGCCGCCACC GGGCCCAGAC GGAGAAGCAT GTGATCCTGT GCGTGAGCTC CCTCAAGATC 
GACCTGCTCA TGGACTTTCT GAACGAGTTC TACGCGCATC CACGGTTGCA GGACTACTAC 
GTCGTCATCC TGTGCCCCAC GGAGATGGAC ATCCAGGTTC GCAGGGTCCT GCAGATCCCA 
CTGTGGTCCC AGCGGGTCAT CTACCTCCAG GGCTCCGCGC TCAAGGATCA GGACCTCATG 
CGGGCCAAGA TGGACAACGG GGAGGCCTGC TTTATTCTCA GCAGCCGGAA CGAGGTGGAC 
CGCACCGCGG CGGACCACCA GACCATACTG CGTGCCTGGG CCGTGAAGGA CTTTGCCCCG 
AACTGCCCCC TCTACGTCCA GATCCTCAAG CCTGAGAACA AGTTTCATGT CAAGTTTGCA 
GAGCACGTGG TGTGCGAGGA GGAGTGCAAG TATGCCATGC TGGCCCTCAA CTGCATCTGC 
CCAGCCACGT CCACCCTCAT CACCCTGCTG GTGCACACGT CCCGCGGCCA GGAAGGCCAG 
GACTCGCCAG AGCAGTGGCA GCGCATGTAC GGACGCTGCT CGGGCAACGA GGTCTACCAC 
GTGCGGATGG GGGACAGCAA GTTCTTCCGC GAGTACGAGG GCAAGAGCTT CACCTACGCC 
GCCTTCCACG CGCACAAGAA GTACGGCGTG TGCCTCATCG GCCTGAAGCG CGAGGACAAC 
AAGAGCATCC TGCTGAACCC GGGCCCACGG CACATCCTGG CGGCCTCCGA CACCTGCTTC 
TACATCAACA TCACCAAGGA GGAGAACTCG GCCTTCATCT TCAAGCAGGA GGAAAAGCAG 
AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG TACGAGGGCT CATCCCGCCT GCCCGTGCAC 
AGCATCATCG CCTCCATGGG GACAGTGGCC ATGGACCTCC AGAACACGGA GTGCCGGCCC 
ACACAGAGTG GCGGGGGCAG CGGGGGCAGC AAGCTGGCGC TCCCCACAGA GAACGGCTCG 
GGCAGCCGGC GGCCCAGCAT CGCGCCCGTG CTGGAGGTGG CTGACAGCTC GGCCCTGCTG 
CCCTGCGACC TGCTGAGTGA CCAGTCAGAG GACGAGATGA CGCCGTCGGA GGACGAGGGG 
CTGTCCATGG TGGAGTATGT GAAGGGCTAC CCTCCTAACT CACCCTACAT CGGCAGCTCC 
CCCACCCTGT GCCACCTCCT GCCTGTGAAA GCCCCCTTCT GCTGCCTGCG GCTGGACAAG 
GGCTGCAAGC ACAACAGCTA CGAAGATGCC AAGGCCTACG GCTTCAAGAA CAAGCTGATC 
ATTGTCTCGG CGGAGACCGC GGGCAATGGG CTGTACAACT TCATCGTGCC GCTGCGCGCC 
TACTACCGGC CTCGCCGGGA GCTCAACCCC ATTGTGCTGC TGCTGGACAA CAAGCCCGAC 
CACCACTTCC TGGAGGCCAT CTGCTGCTTT CCCATGGTCT ACTACATGGA GGGCTCCGTG 
GACAACCTGG ACAGCCTGCT GCGGTGCGGC GTCATCTATG CGGACAATCT GGTGGTGGCA 
GACAAGGAGA GCACCATGAG TGCCGAGGAG GACTACATGG CCGATGCAAA GACCATCGTC 
AACGTGCAGA CCATGTTCCG GCTCTTCCCC AGTCTCAGCA TCACCACCGA GCTCACCCAC 
CCGTCCAACA TGCGGTTCAT GCAGTTTCGC GCCAAGGACA GCTACTCTCT GGCTCTCTCT 
AAACTGGAGA AGAGGGAGCG TGAGAACGGC TCCAACCTGG CCTTCATGTT CCGCCTGCCC 
TTCGCCGCCG GCCGCGTCTT CAGCATCAGC ATGCTGGACA CACTGCTGTA TCAGTCCTTC 
GTGAAGGACT ACATGATCCC CATCACCAGG CTGTTGCTGG GCTTGGACAC CACGCCGGGC 
TCTGGCTACC TCTGTGCGAT GAAGATCTCG GAGGACGACC TGTGGATCCG CACCTACGGC 
CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC GCCGAGATCC CTATCGGCAT CTACAGGACC 
CAATGCCACG TCTTCTCCAC CGAGCCCCCC GACCTCAGGG CCCAGGCCTC ACCCCTCCTC 
CCCGGCCAGT CCCAGGTCTC AGTGAGCGTG GAGGACTGTG AGGACACTCG GGAGGCAAAG 
GGGCCCTGGG GTGCACGGGT GGGCACGAGC GGTGGCAGCA CCCACGGCCG GCACACGGTG 
GGCAGCGACC CAGCCGAGCA CCCGCTCCTG CGGCGCAAGA GCCTCCCGTG GGCGCGGAGG 
CTGAGCCGCA AGGGCTCCCG GCACACGGGG AAAGCGGCAG CCGAGTGGAT CAGCCAACAG 
CGGCTCAGCC TGTACCGGCG CTCGGAGCGG CAGGAGCTCT CAGAGCTGGT CAAGAACCGC 
ATGAAGCACC TGGGGCTGCC CACCACTGGC TACGAGGATG TAGCAAATTT AACAGCCAGT 
GATGTCATGA ATCGGGTAAA CCTGGGATAT TTGCAAGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CATGTGGCCA GCAGCTCCCA GAGCAGGAAG 
AGCAGCTGCA GCAACAAGCT GGCTTCCTGC AACCCCGAGA CGCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261973.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117758.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBu46823
Clone ID Related Accession (Same CDS sequence)	XM_025261973.1
Accession Version	XM_025261973.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3717bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GACGAGATGA ATGACCACCA GAACACCCTC 
TCCTATGTGC TCATCAACCC CCCGCCCGAC ACGCGGCTGG AGCCCAATGA CATTGTATAT 
CTCATCCGCT CCGACCCCTT GGCCCATGTG GCCAGCAGCT CCCAGAGCAG GAAGAGCAGC 
TGCAGCAACA AGCTGGCTTC CTGCAACCCC GAGACGCGGG ACGAGACACA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117758.1
CDS	16..3732
Translation	MYPHCHGCHAASGASGGQAPPRGVRMGVPCSPRPLPESSLCVREPRLPHPSIRPLSALMCPLHSTLRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261973.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261973.1

ORF Insert Sequence:

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GACGAGATGA ATGACCACCA GAACACCCTC 
TCCTATGTGC TCATCAACCC CCCGCCCGAC ACGCGGCTGG AGCCCAATGA CATTGTATAT 
CTCATCCGCT CCGACCCCTT GGCCCATGTG GCCAGCAGCT CCCAGAGCAG GAAGAGCAGC 
TGCAGCAACA AGCTGGCTTC CTGCAACCCC GAGACGCGGG ACGAGACACA GCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261974.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117759.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA.

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CloneID	OBu46824
Clone ID Related Accession (Same CDS sequence)	XM_025261974.1
Accession Version	XM_025261974.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3603bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCCCATG TCACCTACCA 
AGCCTTGCAA GGCTCTGCAC ATCCAAGCCC ACCATCGGGC TGCCCCTGTT CTCTCCTGCC 
CCCGAGGTGA GGAAACTGAG TCTCCTAGAG GGGGTGTTCC CTCCCCTACC CCACCGTGGT 
AAAGTCAGAA CATGCAGCCC GAGAGGTGGA GGGAGGCCCA GCTCTCACCA GCAAGTCAGG 
GACGGGCCTG AGCCAGGGTC TGGGGAGAGG AAGGGACTTC CACCTGAGAG AAGAAGGGCC 
TGGGGTTCCC CCGGAACTGT GACTGTCCTC CCCATCACCC CAGCTGCTCC ATGGTTCCCC 
CCAGTCCTCT GCCCAGCTCC TGTTCCCCTT CCCTGTACCT GCCCCTGCCC CGCTCCTATC 
CCCGGACACC CTTCCCCTCC CAGGCCTCAC CCCTCCTCCC CGGCCAGTCC CAGGTCTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117759.1
CDS	16..3618
Translation	MYPHCHGCHAASGASGGQAPPRGVRMGVPCSPRPLPESSLCVREPRLPHPSIRPLSALMCPLHSTLRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQAPCHLPSLARLCTSKPTIGLPLFSPAPEVRKLSLLEGVFPPLPHRGKVRTCSPRGGGRPSSHQQVRDGPEPGSGERKGLPPERRRAWGSPGTVTVLPITPAAPWFPPVLCPAPVPLPCTCPCPAPIPGHPSPPRPHPSSPASPRSQ

Target ORF information:

RefSeq Version	XM_025261974.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261974.1

ORF Insert Sequence:

ATGTACCCAC ACTGCCACGG GTGCCATGCT GCTTCTGGGG CATCCGGGGG CCAGGCCCCA 
CCACGGGGTG TGCGCATGGG TGTGCCCTGC TCCCCTCGGC CCCTGCCCGA GTCCTCCCTC 
TGTGTCCGGG AGCCCAGGCT CCCCCATCCG TCCATCCGTC CGCTGTCTGC GTTGATGTGT 
CCGCTCCATT CCACCCTCAG GCTTCATGTG GAAGAGTTCA GCCTGGACTC CTCTGTGTCT 
CAGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCCCATG TCACCTACCA 
AGCCTTGCAA GGCTCTGCAC ATCCAAGCCC ACCATCGGGC TGCCCCTGTT CTCTCCTGCC 
CCCGAGGTGA GGAAACTGAG TCTCCTAGAG GGGGTGTTCC CTCCCCTACC CCACCGTGGT 
AAAGTCAGAA CATGCAGCCC GAGAGGTGGA GGGAGGCCCA GCTCTCACCA GCAAGTCAGG 
GACGGGCCTG AGCCAGGGTC TGGGGAGAGG AAGGGACTTC CACCTGAGAG AAGAAGGGCC 
TGGGGTTCCC CCGGAACTGT GACTGTCCTC CCCATCACCC CAGCTGCTCC ATGGTTCCCC 
CCAGTCCTCT GCCCAGCTCC TGTTCCCCTT CCCTGTACCT GCCCCTGCCC CGCTCCTATC 
CCCGGACACC CTTCCCCTCC CAGGCCTCAC CCCTCCTCCC CGGCCAGTCC CAGGTCTCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261975.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117760.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBu46825
Clone ID Related Accession (Same CDS sequence)	XM_025261975.1
Accession Version	XM_025261975.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGGCC 
TCACCCCTCC TCCCCGGCCA GTCCCAGGTC TCAGTGAGCG TGGAGGACTG TGAGGACACT 
CGGGAGGCAA AGGGGCCCTG GGGTGCACGG GTGGGCACGA GCGGTGGCAG CACCCACGGC 
CGGCACACGG TGGGCAGCGA CCCAGCCGAG CACCCGCTCC TGCGGCGCAA GAGCCTCCCG 
TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC CGGCACACGG GGAAAGCGGC AGCCGAGTGG 
ATCAGCCAAC AGCGGCTCAG CCTGTACCGG CGCTCGGAGC GGCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GCATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA TGTAGCAAAT 
TTAACAGCCA GTGATGTCAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA CGAGATGAAT 
GACCACCAGA ACACCCTCTC CTATGTGCTC ATCAACCCCC CGCCCGACAC GCGGCTGGAG 
CCCAATGACA TTGTATATCT CATCCGCTCC GACCCCTTGG CCCATGTGGC CAGCAGCTCC 
CAGAGCAGGA AGAGCAGCTG CAGCAACAAG CTGGCTTCCT GCAACCCCGA GACGCGGGAC 
GAGACACAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117760.1
CDS	1..3735
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261975.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261975.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGGCC 
TCACCCCTCC TCCCCGGCCA GTCCCAGGTC TCAGTGAGCG TGGAGGACTG TGAGGACACT 
CGGGAGGCAA AGGGGCCCTG GGGTGCACGG GTGGGCACGA GCGGTGGCAG CACCCACGGC 
CGGCACACGG TGGGCAGCGA CCCAGCCGAG CACCCGCTCC TGCGGCGCAA GAGCCTCCCG 
TGGGCGCGGA GGCTGAGCCG CAAGGGCTCC CGGCACACGG GGAAAGCGGC AGCCGAGTGG 
ATCAGCCAAC AGCGGCTCAG CCTGTACCGG CGCTCGGAGC GGCAGGAGCT CTCAGAGCTG 
GTCAAGAACC GCATGAAGCA CCTGGGGCTG CCCACCACTG GCTACGAGGA TGTAGCAAAT 
TTAACAGCCA GTGATGTCAT GAATCGGGTA AACCTGGGAT ATTTGCAAGA CGAGATGAAT 
GACCACCAGA ACACCCTCTC CTATGTGCTC ATCAACCCCC CGCCCGACAC GCGGCTGGAG 
CCCAATGACA TTGTATATCT CATCCGCTCC GACCCCTTGG CCCATGTGGC CAGCAGCTCC 
CAGAGCAGGA AGAGCAGCTG CAGCAACAAG CTGGCTTCCT GCAACCCCGA GACGCGGGAC 
GAGACACAGC TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261976.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117761.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA.

Price & Availability↑

CloneID	OBu46826
Clone ID Related Accession (Same CDS sequence)	XM_025261976.1
Accession Version	XM_025261976.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3711bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGAGGATGTA GCAAATTTAA CAGCCAGTGA TGTCATGAAT 
CGGGTAAACC TGGGATATTT GCAAGACGAG ATGAATGACC ACCAGAACAC CCTCTCCTAT 
GTGCTCATCA ACCCCCCGCC CGACACGCGG CTGGAGCCCA ATGACATTGT ATATCTCATC 
CGCTCCGACC CCTTGGCCCA TGTGGCCAGC AGCTCCCAGA GCAGGAAGAG CAGCTGCAGC 
AACAAGCTGG CTTCCTGCAA CCCCGAGACG CGGGACGAGA CACAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117761.1
CDS	1..3711
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261976.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261976.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGAGGATGTA GCAAATTTAA CAGCCAGTGA TGTCATGAAT 
CGGGTAAACC TGGGATATTT GCAAGACGAG ATGAATGACC ACCAGAACAC CCTCTCCTAT 
GTGCTCATCA ACCCCCCGCC CGACACGCGG CTGGAGCCCA ATGACATTGT ATATCTCATC 
CGCTCCGACC CCTTGGCCCA TGTGGCCAGC AGCTCCCAGA GCAGGAAGAG CAGCTGCAGC 
AACAAGCTGG CTTCCTGCAA CCCCGAGACG CGGGACGAGA CACAGCTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261977.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117762.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA.

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CloneID	OBu46827
Clone ID Related Accession (Same CDS sequence)	XM_025261977.1
Accession Version	XM_025261977.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTA TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATT GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC TGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGTGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGCGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCCTGCTGTG CCTGGTCTTC ACGGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACG TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACACCCAAG ATCTGGCCAT CCCAGCTGCT GGTGGTCATC 
ATGATCTGCG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGGTTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAACGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCGG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCG AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAC AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCCGTGCAC AGCATCATCG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAACACGGA GTGCCGGCCC ACACAGAGTG GCGGGGGCAG CGGGGGCAGC 
AAGCTGGCGC TCCCCACAGA GAACGGCTCG GGCAGCCGGC GGCCCAGCAT CGCGCCCGTG 
CTGGAGGTGG CTGACAGCTC GGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGACGAGGGG CTGTCCATGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCCACCCTGT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTACG GCTTCAAGAA CAAGCTGATC ATTGTCTCGG CGGAGACCGC GGGCAATGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGC 
GTCATCTATG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG TGCCGAGGAG 
GACTACATGG CCGATGCAAA GACCATCGTC AACGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCTGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTACGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAATGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGTCCCA GGTCTCAGTG AGCGTGGAGG ACTGTGAGGA CACTCGGGAG 
GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC ACGAGCGGTG GCAGCACCCA CGGCCGGCAC 
ACGGTGGGCA GCGACCCAGC CGAGCACCCG CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG 
CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC ACGGGGAAAG CGGCAGCCGA GTGGATCAGC 
CAACAGCGGC TCAGCCTGTA CCGGCGCTCG GAGCGGCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGCATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGATGTAGC AAATTTAACA 
GCCAGTGATG TCATGAATCG GGTAAACCTG GGATATTTGC AAGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCATG TGGCCAGCAG CTCCCAGAGC 
AGGAAGAGCA GCTGCAGCAA CAAGCTGGCT TCCTGCAACC CCGAGACGCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117762.1
CDS	1..3669
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261977.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261977.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGGTCC AGGTGGAGTT CTACGTCAAC 
GAGAACACCT TCAAGGAGAG GCTTAAGCTA TTCTTCATCA AAAACCAAAG ATCCAGCCTG 
AGGATCCGGC TGTTCAACTT CTCCCTGAAG CTGCTGACCT GCCTGCTGTA CATTGTCCGC 
GTCCTGCTGG ACGACCCAGC CCTGGGGATT GGATGCTGGG GCTGCCCGAA GCAGAATTAC 
ACCTTCAATG AGTCGTCCTC TGAGATCAAC TGGGCCCCCA TCCTGTGGGT GGAGAGGAAG 
GTGGCTCTGT GGGCCATTCA GGTCATCGTG GCCATCATCA GCTTCCTGGA GACCATGCTT 
CTCATTTACC TCAGCTACAA AGGCAACATC TGGGAGCAGA TCTTCCGTGT GTCCTTCATC 
CTGGAGATGA TCAACACGCT GCCCTTCATC ATCACGATAT TCTGGCCGCC GCTGCGGAAC 
CTGTTCATCC CCGTGTTTCT GAACTGCTGG CTGGCCAAGC ACGCCCTGGA GAACATGATC 
AACGACTTCC ACCGCGCCAT CCTGCGCTCG CAGTCGGCCA TGTTCAATCA GGTCCTCATC 
CTCTTCTGCA CCCTGCTGTG CCTGGTCTTC ACGGGGACCT GCGGCATCCA GCACCTGGAG 
CGCGCGGGCG ACAACCTGTC CCTCCTGACG TCCTTCTACT TCTGCATCGT CACCTTCTCC 
ACCGTGGGCT ATGGGGACGT GACACCCAAG ATCTGGCCAT CCCAGCTGCT GGTGGTCATC 
ATGATCTGCG TGGCCCTGGT GGTGCTCCCG CTGCAGTTTG AGGAACTCGT CTACCTGTGG 
ATGGAGCGGC AGAAGTCGGG GGGCAACTAC AGCCGCCACC GGGCCCAGAC GGAGAAGCAT 
GTGATCCTGT GCGTGAGCTC CCTCAAGATC GACCTGCTCA TGGACTTTCT GAACGAGTTC 
TACGCGCATC CACGGTTGCA GGACTACTAC GTCGTCATCC TGTGCCCCAC GGAGATGGAC 
ATCCAGGTTC GCAGGGTCCT GCAGATCCCA CTGTGGTCCC AGCGGGTCAT CTACCTCCAG 
GGCTCCGCGC TCAAGGATCA GGACCTCATG CGGGCCAAGA TGGACAACGG GGAGGCCTGC 
TTTATTCTCA GCAGCCGGAA CGAGGTGGAC CGCACCGCGG CGGACCACCA GACCATACTG 
CGTGCCTGGG CCGTGAAGGA CTTTGCCCCG AACTGCCCCC TCTACGTCCA GATCCTCAAG 
CCTGAGAACA AGTTTCATGT CAAGTTTGCA GAGCACGTGG TGTGCGAGGA GGAGTGCAAG 
TATGCCATGC TGGCCCTCAA CTGCATCTGC CCAGCCACGT CCACCCTCAT CACCCTGCTG 
GTGCACACGT CCCGCGGCCA GGAAGGCCAG GACTCGCCAG AGCAGTGGCA GCGCATGTAC 
GGACGCTGCT CGGGCAACGA GGTCTACCAC GTGCGGATGG GGGACAGCAA GTTCTTCCGC 
GAGTACGAGG GCAAGAGCTT CACCTACGCC GCCTTCCACG CGCACAAGAA GTACGGCGTG 
TGCCTCATCG GCCTGAAGCG CGAGGACAAC AAGAGCATCC TGCTGAACCC GGGCCCACGG 
CACATCCTGG CGGCCTCCGA CACCTGCTTC TACATCAACA TCACCAAGGA GGAGAACTCG 
GCCTTCATCT TCAAGCAGGA GGAAAAGCAG AAGAGGAAGG GCTTCGCGGG GCAGGGGCTG 
TACGAGGGCT CATCCCGCCT GCCCGTGCAC AGCATCATCG CCTCCATGGG GACAGTGGCC 
ATGGACCTCC AGAACACGGA GTGCCGGCCC ACACAGAGTG GCGGGGGCAG CGGGGGCAGC 
AAGCTGGCGC TCCCCACAGA GAACGGCTCG GGCAGCCGGC GGCCCAGCAT CGCGCCCGTG 
CTGGAGGTGG CTGACAGCTC GGCCCTGCTG CCCTGCGACC TGCTGAGTGA CCAGTCAGAG 
GACGAGATGA CGCCGTCGGA GGACGAGGGG CTGTCCATGG TGGAGTATGT GAAGGGCTAC 
CCTCCTAACT CACCCTACAT CGGCAGCTCC CCCACCCTGT GCCACCTCCT GCCTGTGAAA 
GCCCCCTTCT GCTGCCTGCG GCTGGACAAG GGCTGCAAGC ACAACAGCTA CGAAGATGCC 
AAGGCCTACG GCTTCAAGAA CAAGCTGATC ATTGTCTCGG CGGAGACCGC GGGCAATGGG 
CTGTACAACT TCATCGTGCC GCTGCGCGCC TACTACCGGC CTCGCCGGGA GCTCAACCCC 
ATTGTGCTGC TGCTGGACAA CAAGCCCGAC CACCACTTCC TGGAGGCCAT CTGCTGCTTT 
CCCATGGTCT ACTACATGGA GGGCTCCGTG GACAACCTGG ACAGCCTGCT GCGGTGCGGC 
GTCATCTATG CGGACAATCT GGTGGTGGCA GACAAGGAGA GCACCATGAG TGCCGAGGAG 
GACTACATGG CCGATGCAAA GACCATCGTC AACGTGCAGA CCATGTTCCG GCTCTTCCCC 
AGTCTCAGCA TCACCACCGA GCTCACCCAC CCGTCCAACA TGCGGTTCAT GCAGTTTCGC 
GCCAAGGACA GCTACTCTCT GGCTCTCTCT AAACTGGAGA AGAGGGAGCG TGAGAACGGC 
TCCAACCTGG CCTTCATGTT CCGCCTGCCC TTCGCCGCCG GCCGCGTCTT CAGCATCAGC 
ATGCTGGACA CACTGCTGTA TCAGTCCTTC GTGAAGGACT ACATGATCCC CATCACCAGG 
CTGTTGCTGG GCTTGGACAC CACGCCGGGC TCTGGCTACC TCTGTGCGAT GAAGATCTCG 
GAGGACGACC TGTGGATCCG CACCTACGGC CGCCTCTTCC AGAAGCTGTG CTCCTCCAGC 
GCCGAGATCC CTATCGGCAT CTACAGGACC CAATGCCACG TCTTCTCCAC CGAGCCCCCC 
GACCTCAGGG CCCAGTCCCA GGTCTCAGTG AGCGTGGAGG ACTGTGAGGA CACTCGGGAG 
GCAAAGGGGC CCTGGGGTGC ACGGGTGGGC ACGAGCGGTG GCAGCACCCA CGGCCGGCAC 
ACGGTGGGCA GCGACCCAGC CGAGCACCCG CTCCTGCGGC GCAAGAGCCT CCCGTGGGCG 
CGGAGGCTGA GCCGCAAGGG CTCCCGGCAC ACGGGGAAAG CGGCAGCCGA GTGGATCAGC 
CAACAGCGGC TCAGCCTGTA CCGGCGCTCG GAGCGGCAGG AGCTCTCAGA GCTGGTCAAG 
AACCGCATGA AGCACCTGGG GCTGCCCACC ACTGGCTACG AGGATGTAGC AAATTTAACA 
GCCAGTGATG TCATGAATCG GGTAAACCTG GGATATTTGC AAGACGAGAT GAATGACCAC 
CAGAACACCC TCTCCTATGT GCTCATCAAC CCCCCGCCCG ACACGCGGCT GGAGCCCAAT 
GACATTGTAT ATCTCATCCG CTCCGACCCC TTGGCCCATG TGGCCAGCAG CTCCCAGAGC 
AGGAAGAGCA GCTGCAGCAA CAAGCTGGCT TCCTGCAACC CCGAGACGCG GGACGAGACA 
CAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261978.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117763.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OBu46828
Clone ID Related Accession (Same CDS sequence)	XM_025261978.1
Accession Version	XM_025261978.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCATGT GGCCAGCAGC TCCCAGAGCA GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCTT CCTGCAACCC CGAGACGCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117763.1
CDS	1..3648
Translation	MARAKLPRSPSEGKAGPGGTPAGASAPEEPHGLSPLLPARGGGSVGSDVGQRLHVEEFSLDSSVSQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261978.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261978.1

ORF Insert Sequence:

ATGGCGCGGG CCAAGCTGCC GCGCTCGCCG TCCGAGGGCA AGGCGGGCCC GGGGGGCACC 
CCAGCCGGCG CCTCGGCCCC CGAGGAGCCG CACGGGCTCA GCCCGCTGCT GCCGGCCCGC 
GGCGGGGGCT CCGTGGGCAG CGACGTGGGC CAGAGGCTTC ATGTGGAAGA GTTCAGCCTG 
GACTCCTCTG TGTCTCAGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCATGT GGCCAGCAGC TCCCAGAGCA GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCTT CCTGCAACCC CGAGACGCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261979.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117764.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA.

Price & Availability↑

CloneID	OBu46829
Clone ID Related Accession (Same CDS sequence)	XM_025261979.1
Accession Version	XM_025261979.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAT GTGGCCAGCA GCTCCCAGAG CAGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC TTCCTGCAAC CCCGAGACGC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117764.1
CDS	1267..4806
Translation	MVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQASPLLPGQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYEDVANLTASDVMNRVNLGYLQDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261979.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261979.1

ORF Insert Sequence:

ATGGTCCAGG TGGAGTTCTA CGTCAACGAG AACACCTTCA AGGAGAGGCT TAAGCTATTC 
TTCATCAAAA ACCAAAGATC CAGCCTGAGG ATCCGGCTGT TCAACTTCTC CCTGAAGCTG 
CTGACCTGCC TGCTGTACAT TGTCCGCGTC CTGCTGGACG ACCCAGCCCT GGGGATTGGA 
TGCTGGGGCT GCCCGAAGCA GAATTACACC TTCAATGAGT CGTCCTCTGA GATCAACTGG 
GCCCCCATCC TGTGGGTGGA GAGGAAGGTG GCTCTGTGGG CCATTCAGGT CATCGTGGCC 
ATCATCAGCT TCCTGGAGAC CATGCTTCTC ATTTACCTCA GCTACAAAGG CAACATCTGG 
GAGCAGATCT TCCGTGTGTC CTTCATCCTG GAGATGATCA ACACGCTGCC CTTCATCATC 
ACGATATTCT GGCCGCCGCT GCGGAACCTG TTCATCCCCG TGTTTCTGAA CTGCTGGCTG 
GCCAAGCACG CCCTGGAGAA CATGATCAAC GACTTCCACC GCGCCATCCT GCGCTCGCAG 
TCGGCCATGT TCAATCAGGT CCTCATCCTC TTCTGCACCC TGCTGTGCCT GGTCTTCACG 
GGGACCTGCG GCATCCAGCA CCTGGAGCGC GCGGGCGACA ACCTGTCCCT CCTGACGTCC 
TTCTACTTCT GCATCGTCAC CTTCTCCACC GTGGGCTATG GGGACGTGAC ACCCAAGATC 
TGGCCATCCC AGCTGCTGGT GGTCATCATG ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG 
CAGTTTGAGG AACTCGTCTA CCTGTGGATG GAGCGGCAGA AGTCGGGGGG CAACTACAGC 
CGCCACCGGG CCCAGACGGA GAAGCATGTG ATCCTGTGCG TGAGCTCCCT CAAGATCGAC 
CTGCTCATGG ACTTTCTGAA CGAGTTCTAC GCGCATCCAC GGTTGCAGGA CTACTACGTC 
GTCATCCTGT GCCCCACGGA GATGGACATC CAGGTTCGCA GGGTCCTGCA GATCCCACTG 
TGGTCCCAGC GGGTCATCTA CCTCCAGGGC TCCGCGCTCA AGGATCAGGA CCTCATGCGG 
GCCAAGATGG ACAACGGGGA GGCCTGCTTT ATTCTCAGCA GCCGGAACGA GGTGGACCGC 
ACCGCGGCGG ACCACCAGAC CATACTGCGT GCCTGGGCCG TGAAGGACTT TGCCCCGAAC 
TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT GAGAACAAGT TTCATGTCAA GTTTGCAGAG 
CACGTGGTGT GCGAGGAGGA GTGCAAGTAT GCCATGCTGG CCCTCAACTG CATCTGCCCA 
GCCACGTCCA CCCTCATCAC CCTGCTGGTG CACACGTCCC GCGGCCAGGA AGGCCAGGAC 
TCGCCAGAGC AGTGGCAGCG CATGTACGGA CGCTGCTCGG GCAACGAGGT CTACCACGTG 
CGGATGGGGG ACAGCAAGTT CTTCCGCGAG TACGAGGGCA AGAGCTTCAC CTACGCCGCC 
TTCCACGCGC ACAAGAAGTA CGGCGTGTGC CTCATCGGCC TGAAGCGCGA GGACAACAAG 
AGCATCCTGC TGAACCCGGG CCCACGGCAC ATCCTGGCGG CCTCCGACAC CTGCTTCTAC 
ATCAACATCA CCAAGGAGGA GAACTCGGCC TTCATCTTCA AGCAGGAGGA AAAGCAGAAG 
AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC GAGGGCTCAT CCCGCCTGCC CGTGCACAGC 
ATCATCGCCT CCATGGGGAC AGTGGCCATG GACCTCCAGA ACACGGAGTG CCGGCCCACA 
CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG CTGGCGCTCC CCACAGAGAA CGGCTCGGGC 
AGCCGGCGGC CCAGCATCGC GCCCGTGCTG GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC 
TGCGACCTGC TGAGTGACCA GTCAGAGGAC GAGATGACGC CGTCGGAGGA CGAGGGGCTG 
TCCATGGTGG AGTATGTGAA GGGCTACCCT CCTAACTCAC CCTACATCGG CAGCTCCCCC 
ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC CCCTTCTGCT GCCTGCGGCT GGACAAGGGC 
TGCAAGCACA ACAGCTACGA AGATGCCAAG GCCTACGGCT TCAAGAACAA GCTGATCATT 
GTCTCGGCGG AGACCGCGGG CAATGGGCTG TACAACTTCA TCGTGCCGCT GCGCGCCTAC 
TACCGGCCTC GCCGGGAGCT CAACCCCATT GTGCTGCTGC TGGACAACAA GCCCGACCAC 
CACTTCCTGG AGGCCATCTG CTGCTTTCCC ATGGTCTACT ACATGGAGGG CTCCGTGGAC 
AACCTGGACA GCCTGCTGCG GTGCGGCGTC ATCTATGCGG ACAATCTGGT GGTGGCAGAC 
AAGGAGAGCA CCATGAGTGC CGAGGAGGAC TACATGGCCG ATGCAAAGAC CATCGTCAAC 
GTGCAGACCA TGTTCCGGCT CTTCCCCAGT CTCAGCATCA CCACCGAGCT CACCCACCCG 
TCCAACATGC GGTTCATGCA GTTTCGCGCC AAGGACAGCT ACTCTCTGGC TCTCTCTAAA 
CTGGAGAAGA GGGAGCGTGA GAACGGCTCC AACCTGGCCT TCATGTTCCG CCTGCCCTTC 
GCCGCCGGCC GCGTCTTCAG CATCAGCATG CTGGACACAC TGCTGTATCA GTCCTTCGTG 
AAGGACTACA TGATCCCCAT CACCAGGCTG TTGCTGGGCT TGGACACCAC GCCGGGCTCT 
GGCTACCTCT GTGCGATGAA GATCTCGGAG GACGACCTGT GGATCCGCAC CTACGGCCGC 
CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC GAGATCCCTA TCGGCATCTA CAGGACCCAA 
TGCCACGTCT TCTCCACCGA GCCCCCCGAC CTCAGGGCCC AGGCCTCACC CCTCCTCCCC 
GGCCAGTCCC AGGTCTCAGT GAGCGTGGAG GACTGTGAGG ACACTCGGGA GGCAAAGGGG 
CCCTGGGGTG CACGGGTGGG CACGAGCGGT GGCAGCACCC ACGGCCGGCA CACGGTGGGC 
AGCGACCCAG CCGAGCACCC GCTCCTGCGG CGCAAGAGCC TCCCGTGGGC GCGGAGGCTG 
AGCCGCAAGG GCTCCCGGCA CACGGGGAAA GCGGCAGCCG AGTGGATCAG CCAACAGCGG 
CTCAGCCTGT ACCGGCGCTC GGAGCGGCAG GAGCTCTCAG AGCTGGTCAA GAACCGCATG 
AAGCACCTGG GGCTGCCCAC CACTGGCTAC GAGGATGTAG CAAATTTAAC AGCCAGTGAT 
GTCATGAATC GGGTAAACCT GGGATATTTG CAAGACGAGA TGAATGACCA CCAGAACACC 
CTCTCCTATG TGCTCATCAA CCCCCCGCCC GACACGCGGC TGGAGCCCAA TGACATTGTA 
TATCTCATCC GCTCCGACCC CTTGGCCCAT GTGGCCAGCA GCTCCCAGAG CAGGAAGAGC 
AGCTGCAGCA ACAAGCTGGC TTCCTGCAAC CCCGAGACGC GGGACGAGAC ACAGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_025261980.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_025117765.1 Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA.

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CloneID	OBu46830
Clone ID Related Accession (Same CDS sequence)	XM_025261980.1
Accession Version	XM_025261980.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTTC CCGACAGGGT GCACAGGGGT CCAGGAGATG TCTACAGAGG GGCACAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCAGCCC 
GGCGCCGAGG ACGGCGGCCG CGGGGACGGC GCGCGCCTGG ACACCGCCGG CCTCAAGATG 
AGCGACCTGG ACTCGGAGGT GCTGCCCTTG CCTCCGCGGT ACCGCTTCCG GGACCTGCTG 
CTGGGCGACC AGTCCTTCCA GAACGACGAC AGGGTCCAGG TGGAGTTCTA CGTCAACGAG 
AACACCTTCA AGGAGAGGCT TAAGCTATTC TTCATCAAAA ACCAAAGATC CAGCCTGAGG 
ATCCGGCTGT TCAACTTCTC CCTGAAGCTG CTGACCTGCC TGCTGTACAT TGTCCGCGTC 
CTGCTGGACG ACCCAGCCCT GGGGATTGGA TGCTGGGGCT GCCCGAAGCA GAATTACACC 
TTCAATGAGT CGTCCTCTGA GATCAACTGG GCCCCCATCC TGTGGGTGGA GAGGAAGGTG 
GCTCTGTGGG CCATTCAGGT CATCGTGGCC ATCATCAGCT TCCTGGAGAC CATGCTTCTC 
ATTTACCTCA GCTACAAAGG CAACATCTGG GAGCAGATCT TCCGTGTGTC CTTCATCCTG 
GAGATGATCA ACACGCTGCC CTTCATCATC ACGATATTCT GGCCGCCGCT GCGGAACCTG 
TTCATCCCCG TGTTTCTGAA CTGCTGGCTG GCCAAGCACG CCCTGGAGAA CATGATCAAC 
GACTTCCACC GCGCCATCCT GCGCTCGCAG TCGGCCATGT TCAATCAGGT CCTCATCCTC 
TTCTGCACCC TGCTGTGCCT GGTCTTCACG GGGACCTGCG GCATCCAGCA CCTGGAGCGC 
GCGGGCGACA ACCTGTCCCT CCTGACGTCC TTCTACTTCT GCATCGTCAC CTTCTCCACC 
GTGGGCTATG GGGACGTGAC ACCCAAGATC TGGCCATCCC AGCTGCTGGT GGTCATCATG 
ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG CAGTTTGAGG AACTCGTCTA CCTGTGGATG 
GAGCGGCAGA AGTCGGGGGG CAACTACAGC CGCCACCGGG CCCAGACGGA GAAGCATGTG 
ATCCTGTGCG TGAGCTCCCT CAAGATCGAC CTGCTCATGG ACTTTCTGAA CGAGTTCTAC 
GCGCATCCAC GGTTGCAGGA CTACTACGTC GTCATCCTGT GCCCCACGGA GATGGACATC 
CAGGTTCGCA GGGTCCTGCA GATCCCACTG TGGTCCCAGC GGGTCATCTA CCTCCAGGGC 
TCCGCGCTCA AGGATCAGGA CCTCATGCGG GCCAAGATGG ACAACGGGGA GGCCTGCTTT 
ATTCTCAGCA GCCGGAACGA GGTGGACCGC ACCGCGGCGG ACCACCAGAC CATACTGCGT 
GCCTGGGCCG TGAAGGACTT TGCCCCGAAC TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT 
GAGAACAAGT TTCATGTCAA GTTTGCAGAG CACGTGGTGT GCGAGGAGGA GTGCAAGTAT 
GCCATGCTGG CCCTCAACTG CATCTGCCCA GCCACGTCCA CCCTCATCAC CCTGCTGGTG 
CACACGTCCC GCGGCCAGGA AGGCCAGGAC TCGCCAGAGC AGTGGCAGCG CATGTACGGA 
CGCTGCTCGG GCAACGAGGT CTACCACGTG CGGATGGGGG ACAGCAAGTT CTTCCGCGAG 
TACGAGGGCA AGAGCTTCAC CTACGCCGCC TTCCACGCGC ACAAGAAGTA CGGCGTGTGC 
CTCATCGGCC TGAAGCGCGA GGACAACAAG AGCATCCTGC TGAACCCGGG CCCACGGCAC 
ATCCTGGCGG CCTCCGACAC CTGCTTCTAC ATCAACATCA CCAAGGAGGA GAACTCGGCC 
TTCATCTTCA AGCAGGAGGA AAAGCAGAAG AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC 
GAGGGCTCAT CCCGCCTGCC CGTGCACAGC ATCATCGCCT CCATGGGGAC AGTGGCCATG 
GACCTCCAGA ACACGGAGTG CCGGCCCACA CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG 
CTGGCGCTCC CCACAGAGAA CGGCTCGGGC AGCCGGCGGC CCAGCATCGC GCCCGTGCTG 
GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAC 
GAGATGACGC CGTCGGAGGA CGAGGGGCTG TCCATGGTGG AGTATGTGAA GGGCTACCCT 
CCTAACTCAC CCTACATCGG CAGCTCCCCC ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC 
CCCTTCTGCT GCCTGCGGCT GGACAAGGGC TGCAAGCACA ACAGCTACGA AGATGCCAAG 
GCCTACGGCT TCAAGAACAA GCTGATCATT GTCTCGGCGG AGACCGCGGG CAATGGGCTG 
TACAACTTCA TCGTGCCGCT GCGCGCCTAC TACCGGCCTC GCCGGGAGCT CAACCCCATT 
GTGCTGCTGC TGGACAACAA GCCCGACCAC CACTTCCTGG AGGCCATCTG CTGCTTTCCC 
ATGGTCTACT ACATGGAGGG CTCCGTGGAC AACCTGGACA GCCTGCTGCG GTGCGGCGTC 
ATCTATGCGG ACAATCTGGT GGTGGCAGAC AAGGAGAGCA CCATGAGTGC CGAGGAGGAC 
TACATGGCCG ATGCAAAGAC CATCGTCAAC GTGCAGACCA TGTTCCGGCT CTTCCCCAGT 
CTCAGCATCA CCACCGAGCT CACCCACCCG TCCAACATGC GGTTCATGCA GTTTCGCGCC 
AAGGACAGCT ACTCTCTGGC TCTCTCTAAA CTGGAGAAGA GGGAGCGTGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCCTTC GCCGCCGGCC GCGTCTTCAG CATCAGCATG 
CTGGACACAC TGCTGTATCA GTCCTTCGTG AAGGACTACA TGATCCCCAT CACCAGGCTG 
TTGCTGGGCT TGGACACCAC GCCGGGCTCT GGCTACCTCT GTGCGATGAA GATCTCGGAG 
GACGACCTGT GGATCCGCAC CTACGGCCGC CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC 
GAGATCCCTA TCGGCATCTA CAGGACCCAA TGCCACGTCT TCTCCACCGA GCCCCCCGAC 
CTCAGGGCCC AGTCCCAGGT CTCAGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGTGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAAAGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCGGAG CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CATGTGGCCA GCAGCTCCCA GAGCAGGAAG 
AGCAGCTGCA GCAACAAGCT GGCTTCCTGC AACCCCGAGA CGCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_025117765.1
CDS	81..3803
Translation	MPLPDRVHRGPGDVYRGAQGGGHTNRTFVFDDGQCAPRQPGAEDGGRGDGARLDTAGLKMSDLDSEVLPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_025261980.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium sodium-activated channel subfamily T member 1 (KCNT1), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_025261980.1

ORF Insert Sequence:

ATGCCGCTTC CCGACAGGGT GCACAGGGGT CCAGGAGATG TCTACAGAGG GGCACAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCAGCCC 
GGCGCCGAGG ACGGCGGCCG CGGGGACGGC GCGCGCCTGG ACACCGCCGG CCTCAAGATG 
AGCGACCTGG ACTCGGAGGT GCTGCCCTTG CCTCCGCGGT ACCGCTTCCG GGACCTGCTG 
CTGGGCGACC AGTCCTTCCA GAACGACGAC AGGGTCCAGG TGGAGTTCTA CGTCAACGAG 
AACACCTTCA AGGAGAGGCT TAAGCTATTC TTCATCAAAA ACCAAAGATC CAGCCTGAGG 
ATCCGGCTGT TCAACTTCTC CCTGAAGCTG CTGACCTGCC TGCTGTACAT TGTCCGCGTC 
CTGCTGGACG ACCCAGCCCT GGGGATTGGA TGCTGGGGCT GCCCGAAGCA GAATTACACC 
TTCAATGAGT CGTCCTCTGA GATCAACTGG GCCCCCATCC TGTGGGTGGA GAGGAAGGTG 
GCTCTGTGGG CCATTCAGGT CATCGTGGCC ATCATCAGCT TCCTGGAGAC CATGCTTCTC 
ATTTACCTCA GCTACAAAGG CAACATCTGG GAGCAGATCT TCCGTGTGTC CTTCATCCTG 
GAGATGATCA ACACGCTGCC CTTCATCATC ACGATATTCT GGCCGCCGCT GCGGAACCTG 
TTCATCCCCG TGTTTCTGAA CTGCTGGCTG GCCAAGCACG CCCTGGAGAA CATGATCAAC 
GACTTCCACC GCGCCATCCT GCGCTCGCAG TCGGCCATGT TCAATCAGGT CCTCATCCTC 
TTCTGCACCC TGCTGTGCCT GGTCTTCACG GGGACCTGCG GCATCCAGCA CCTGGAGCGC 
GCGGGCGACA ACCTGTCCCT CCTGACGTCC TTCTACTTCT GCATCGTCAC CTTCTCCACC 
GTGGGCTATG GGGACGTGAC ACCCAAGATC TGGCCATCCC AGCTGCTGGT GGTCATCATG 
ATCTGCGTGG CCCTGGTGGT GCTCCCGCTG CAGTTTGAGG AACTCGTCTA CCTGTGGATG 
GAGCGGCAGA AGTCGGGGGG CAACTACAGC CGCCACCGGG CCCAGACGGA GAAGCATGTG 
ATCCTGTGCG TGAGCTCCCT CAAGATCGAC CTGCTCATGG ACTTTCTGAA CGAGTTCTAC 
GCGCATCCAC GGTTGCAGGA CTACTACGTC GTCATCCTGT GCCCCACGGA GATGGACATC 
CAGGTTCGCA GGGTCCTGCA GATCCCACTG TGGTCCCAGC GGGTCATCTA CCTCCAGGGC 
TCCGCGCTCA AGGATCAGGA CCTCATGCGG GCCAAGATGG ACAACGGGGA GGCCTGCTTT 
ATTCTCAGCA GCCGGAACGA GGTGGACCGC ACCGCGGCGG ACCACCAGAC CATACTGCGT 
GCCTGGGCCG TGAAGGACTT TGCCCCGAAC TGCCCCCTCT ACGTCCAGAT CCTCAAGCCT 
GAGAACAAGT TTCATGTCAA GTTTGCAGAG CACGTGGTGT GCGAGGAGGA GTGCAAGTAT 
GCCATGCTGG CCCTCAACTG CATCTGCCCA GCCACGTCCA CCCTCATCAC CCTGCTGGTG 
CACACGTCCC GCGGCCAGGA AGGCCAGGAC TCGCCAGAGC AGTGGCAGCG CATGTACGGA 
CGCTGCTCGG GCAACGAGGT CTACCACGTG CGGATGGGGG ACAGCAAGTT CTTCCGCGAG 
TACGAGGGCA AGAGCTTCAC CTACGCCGCC TTCCACGCGC ACAAGAAGTA CGGCGTGTGC 
CTCATCGGCC TGAAGCGCGA GGACAACAAG AGCATCCTGC TGAACCCGGG CCCACGGCAC 
ATCCTGGCGG CCTCCGACAC CTGCTTCTAC ATCAACATCA CCAAGGAGGA GAACTCGGCC 
TTCATCTTCA AGCAGGAGGA AAAGCAGAAG AGGAAGGGCT TCGCGGGGCA GGGGCTGTAC 
GAGGGCTCAT CCCGCCTGCC CGTGCACAGC ATCATCGCCT CCATGGGGAC AGTGGCCATG 
GACCTCCAGA ACACGGAGTG CCGGCCCACA CAGAGTGGCG GGGGCAGCGG GGGCAGCAAG 
CTGGCGCTCC CCACAGAGAA CGGCTCGGGC AGCCGGCGGC CCAGCATCGC GCCCGTGCTG 
GAGGTGGCTG ACAGCTCGGC CCTGCTGCCC TGCGACCTGC TGAGTGACCA GTCAGAGGAC 
GAGATGACGC CGTCGGAGGA CGAGGGGCTG TCCATGGTGG AGTATGTGAA GGGCTACCCT 
CCTAACTCAC CCTACATCGG CAGCTCCCCC ACCCTGTGCC ACCTCCTGCC TGTGAAAGCC 
CCCTTCTGCT GCCTGCGGCT GGACAAGGGC TGCAAGCACA ACAGCTACGA AGATGCCAAG 
GCCTACGGCT TCAAGAACAA GCTGATCATT GTCTCGGCGG AGACCGCGGG CAATGGGCTG 
TACAACTTCA TCGTGCCGCT GCGCGCCTAC TACCGGCCTC GCCGGGAGCT CAACCCCATT 
GTGCTGCTGC TGGACAACAA GCCCGACCAC CACTTCCTGG AGGCCATCTG CTGCTTTCCC 
ATGGTCTACT ACATGGAGGG CTCCGTGGAC AACCTGGACA GCCTGCTGCG GTGCGGCGTC 
ATCTATGCGG ACAATCTGGT GGTGGCAGAC AAGGAGAGCA CCATGAGTGC CGAGGAGGAC 
TACATGGCCG ATGCAAAGAC CATCGTCAAC GTGCAGACCA TGTTCCGGCT CTTCCCCAGT 
CTCAGCATCA CCACCGAGCT CACCCACCCG TCCAACATGC GGTTCATGCA GTTTCGCGCC 
AAGGACAGCT ACTCTCTGGC TCTCTCTAAA CTGGAGAAGA GGGAGCGTGA GAACGGCTCC 
AACCTGGCCT TCATGTTCCG CCTGCCCTTC GCCGCCGGCC GCGTCTTCAG CATCAGCATG 
CTGGACACAC TGCTGTATCA GTCCTTCGTG AAGGACTACA TGATCCCCAT CACCAGGCTG 
TTGCTGGGCT TGGACACCAC GCCGGGCTCT GGCTACCTCT GTGCGATGAA GATCTCGGAG 
GACGACCTGT GGATCCGCAC CTACGGCCGC CTCTTCCAGA AGCTGTGCTC CTCCAGCGCC 
GAGATCCCTA TCGGCATCTA CAGGACCCAA TGCCACGTCT TCTCCACCGA GCCCCCCGAC 
CTCAGGGCCC AGTCCCAGGT CTCAGTGAGC GTGGAGGACT GTGAGGACAC TCGGGAGGCA 
AAGGGGCCCT GGGGTGCACG GGTGGGCACG AGCGGTGGCA GCACCCACGG CCGGCACACG 
GTGGGCAGCG ACCCAGCCGA GCACCCGCTC CTGCGGCGCA AGAGCCTCCC GTGGGCGCGG 
AGGCTGAGCC GCAAGGGCTC CCGGCACACG GGGAAAGCGG CAGCCGAGTG GATCAGCCAA 
CAGCGGCTCA GCCTGTACCG GCGCTCGGAG CGGCAGGAGC TCTCAGAGCT GGTCAAGAAC 
CGCATGAAGC ACCTGGGGCT GCCCACCACT GGCTACGACG AGATGAATGA CCACCAGAAC 
ACCCTCTCCT ATGTGCTCAT CAACCCCCCG CCCGACACGC GGCTGGAGCC CAATGACATT 
GTATATCTCA TCCGCTCCGA CCCCTTGGCC CATGTGGCCA GCAGCTCCCA GAGCAGGAAG 
AGCAGCTGCA GCAACAAGCT GGCTTCCTGC AACCCCGAGA CGCGGGACGA GACACAGCTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

KCNT1 ( XM_006062186.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006062248.1 Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

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CloneID	OBu18305
Clone ID Related Accession (Same CDS sequence)	XM_006062186.1
Accession Version	XM_006062186.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005785044.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCGCTTC CCGACAGGGT GCACAGGGGT CCAGGAGATG TCTACAGAGG GGCACAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCGCCCC 
TGCAGCGGGG ACGGCGCGCG CCTGGACACC GCCGGCCTCA AGATGAGCGA CCTGGACTCG 
GAGGTGCTGC CCTTGCCTCC GCGGTACCGC TTCCGGGACC TGCTGCTGGG CGACCAGTCC 
TTCCAGAACG ACGACAGGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCATGT GGCCAGCAGC TCCCAGAGCA GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCTT CCTGCAACCC CGAGACGCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006062248.1
CDS	1..3708
Translation	MPLPDRVHRGPGDVYRGAQGGGHTNRTFVFDDGQCAPRRPCSGDGARLDTAGLKMSDLDSEVLPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNFSLKLLTCLLYIVRVLLDDPALGIGCWGCPKQNYTFNESSSEINWAPILWVERKVALWAIQVIVAIISFLETMLLIYLSYKGNIWEQIFRVSFILEMINTLPFIITIFWPPLRNLFIPVFLNCWLAKHALENMINDFHRAILRSQSAMFNQVLILFCTLLCLVFTGTCGIQHLERAGDNLSLLTSFYFCIVTFSTVGYGDVTPKIWPSQLLVVIMICVALVVLPLQFEELVYLWMERQKSGGNYSRHRAQTEKHVILCVSSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRVLQIPLWSQRVIYLQGSALKDQDLMRAKMDNGEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAEHVVCEEECKYAMLALNCICPATSTLITLLVHTSRGQEGQDSPEQWQRMYGRCSGNEVYHVRMGDSKFFREYEGKSFTYAAFHAHKKYGVCLIGLKREDNKSILLNPGPRHILAASDTCFYINITKEENSAFIFKQEEKQKRKGFAGQGLYEGSSRLPVHSIIASMGTVAMDLQNTECRPTQSGGGSGGSKLALPTENGSGSRRPSIAPVLEVADSSALLPCDLLSDQSEDEMTPSEDEGLSMVEYVKGYPPNSPYIGSSPTLCHLLPVKAPFCCLRLDKGCKHNSYEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRRELNPIVLLLDNKPDHHFLEAICCFPMVYYMEGSVDNLDSLLRCGVIYADNLVVADKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSITTELTHPSNMRFMQFRAKDSYSLALSKLEKRERENGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMIPITRLLLGLDTTPGSGYLCAMKISEDDLWIRTYGRLFQKLCSSSAEIPIGIYRTQCHVFSTEPPDLRAQSQVSVSVEDCEDTREAKGPWGARVGTSGGSTHGRHTVGSDPAEHPLLRRKSLPWARRLSRKGSRHTGKAAAEWISQQRLSLYRRSERQELSELVKNRMKHLGLPTTGYDEMNDHQNTLSYVLINPPPDTRLEPNDIVYLIRSDPLAHVASSSQSRKSSCSNKLASCNPETRDETQL

Target ORF information:

RefSeq Version	XM_006062186.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis potassium channel, subfamily T, member 1 (KCNT1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006062186.1

ORF Insert Sequence:

ATGCCGCTTC CCGACAGGGT GCACAGGGGT CCAGGAGATG TCTACAGAGG GGCACAAGGC 
GGGGGCCACA CCAACCGGAC CTTCGTGTTC GACGACGGCC AGTGCGCCCC CAGGCGCCCC 
TGCAGCGGGG ACGGCGCGCG CCTGGACACC GCCGGCCTCA AGATGAGCGA CCTGGACTCG 
GAGGTGCTGC CCTTGCCTCC GCGGTACCGC TTCCGGGACC TGCTGCTGGG CGACCAGTCC 
TTCCAGAACG ACGACAGGGT CCAGGTGGAG TTCTACGTCA ACGAGAACAC CTTCAAGGAG 
AGGCTTAAGC TATTCTTCAT CAAAAACCAA AGATCCAGCC TGAGGATCCG GCTGTTCAAC 
TTCTCCCTGA AGCTGCTGAC CTGCCTGCTG TACATTGTCC GCGTCCTGCT GGACGACCCA 
GCCCTGGGGA TTGGATGCTG GGGCTGCCCG AAGCAGAATT ACACCTTCAA TGAGTCGTCC 
TCTGAGATCA ACTGGGCCCC CATCCTGTGG GTGGAGAGGA AGGTGGCTCT GTGGGCCATT 
CAGGTCATCG TGGCCATCAT CAGCTTCCTG GAGACCATGC TTCTCATTTA CCTCAGCTAC 
AAAGGCAACA TCTGGGAGCA GATCTTCCGT GTGTCCTTCA TCCTGGAGAT GATCAACACG 
CTGCCCTTCA TCATCACGAT ATTCTGGCCG CCGCTGCGGA ACCTGTTCAT CCCCGTGTTT 
CTGAACTGCT GGCTGGCCAA GCACGCCCTG GAGAACATGA TCAACGACTT CCACCGCGCC 
ATCCTGCGCT CGCAGTCGGC CATGTTCAAT CAGGTCCTCA TCCTCTTCTG CACCCTGCTG 
TGCCTGGTCT TCACGGGGAC CTGCGGCATC CAGCACCTGG AGCGCGCGGG CGACAACCTG 
TCCCTCCTGA CGTCCTTCTA CTTCTGCATC GTCACCTTCT CCACCGTGGG CTATGGGGAC 
GTGACACCCA AGATCTGGCC ATCCCAGCTG CTGGTGGTCA TCATGATCTG CGTGGCCCTG 
GTGGTGCTCC CGCTGCAGTT TGAGGAACTC GTCTACCTGT GGATGGAGCG GCAGAAGTCG 
GGGGGCAACT ACAGCCGCCA CCGGGCCCAG ACGGAGAAGC ATGTGATCCT GTGCGTGAGC 
TCCCTCAAGA TCGACCTGCT CATGGACTTT CTGAACGAGT TCTACGCGCA TCCACGGTTG 
CAGGACTACT ACGTCGTCAT CCTGTGCCCC ACGGAGATGG ACATCCAGGT TCGCAGGGTC 
CTGCAGATCC CACTGTGGTC CCAGCGGGTC ATCTACCTCC AGGGCTCCGC GCTCAAGGAT 
CAGGACCTCA TGCGGGCCAA GATGGACAAC GGGGAGGCCT GCTTTATTCT CAGCAGCCGG 
AACGAGGTGG ACCGCACCGC GGCGGACCAC CAGACCATAC TGCGTGCCTG GGCCGTGAAG 
GACTTTGCCC CGAACTGCCC CCTCTACGTC CAGATCCTCA AGCCTGAGAA CAAGTTTCAT 
GTCAAGTTTG CAGAGCACGT GGTGTGCGAG GAGGAGTGCA AGTATGCCAT GCTGGCCCTC 
AACTGCATCT GCCCAGCCAC GTCCACCCTC ATCACCCTGC TGGTGCACAC GTCCCGCGGC 
CAGGAAGGCC AGGACTCGCC AGAGCAGTGG CAGCGCATGT ACGGACGCTG CTCGGGCAAC 
GAGGTCTACC ACGTGCGGAT GGGGGACAGC AAGTTCTTCC GCGAGTACGA GGGCAAGAGC 
TTCACCTACG CCGCCTTCCA CGCGCACAAG AAGTACGGCG TGTGCCTCAT CGGCCTGAAG 
CGCGAGGACA ACAAGAGCAT CCTGCTGAAC CCGGGCCCAC GGCACATCCT GGCGGCCTCC 
GACACCTGCT TCTACATCAA CATCACCAAG GAGGAGAACT CGGCCTTCAT CTTCAAGCAG 
GAGGAAAAGC AGAAGAGGAA GGGCTTCGCG GGGCAGGGGC TGTACGAGGG CTCATCCCGC 
CTGCCCGTGC ACAGCATCAT CGCCTCCATG GGGACAGTGG CCATGGACCT CCAGAACACG 
GAGTGCCGGC CCACACAGAG TGGCGGGGGC AGCGGGGGCA GCAAGCTGGC GCTCCCCACA 
GAGAACGGCT CGGGCAGCCG GCGGCCCAGC ATCGCGCCCG TGCTGGAGGT GGCTGACAGC 
TCGGCCCTGC TGCCCTGCGA CCTGCTGAGT GACCAGTCAG AGGACGAGAT GACGCCGTCG 
GAGGACGAGG GGCTGTCCAT GGTGGAGTAT GTGAAGGGCT ACCCTCCTAA CTCACCCTAC 
ATCGGCAGCT CCCCCACCCT GTGCCACCTC CTGCCTGTGA AAGCCCCCTT CTGCTGCCTG 
CGGCTGGACA AGGGCTGCAA GCACAACAGC TACGAAGATG CCAAGGCCTA CGGCTTCAAG 
AACAAGCTGA TCATTGTCTC GGCGGAGACC GCGGGCAATG GGCTGTACAA CTTCATCGTG 
CCGCTGCGCG CCTACTACCG GCCTCGCCGG GAGCTCAACC CCATTGTGCT GCTGCTGGAC 
AACAAGCCCG ACCACCACTT CCTGGAGGCC ATCTGCTGCT TTCCCATGGT CTACTACATG 
GAGGGCTCCG TGGACAACCT GGACAGCCTG CTGCGGTGCG GCGTCATCTA TGCGGACAAT 
CTGGTGGTGG CAGACAAGGA GAGCACCATG AGTGCCGAGG AGGACTACAT GGCCGATGCA 
AAGACCATCG TCAACGTGCA GACCATGTTC CGGCTCTTCC CCAGTCTCAG CATCACCACC 
GAGCTCACCC ACCCGTCCAA CATGCGGTTC ATGCAGTTTC GCGCCAAGGA CAGCTACTCT 
CTGGCTCTCT CTAAACTGGA GAAGAGGGAG CGTGAGAACG GCTCCAACCT GGCCTTCATG 
TTCCGCCTGC CCTTCGCCGC CGGCCGCGTC TTCAGCATCA GCATGCTGGA CACACTGCTG 
TATCAGTCCT TCGTGAAGGA CTACATGATC CCCATCACCA GGCTGTTGCT GGGCTTGGAC 
ACCACGCCGG GCTCTGGCTA CCTCTGTGCG ATGAAGATCT CGGAGGACGA CCTGTGGATC 
CGCACCTACG GCCGCCTCTT CCAGAAGCTG TGCTCCTCCA GCGCCGAGAT CCCTATCGGC 
ATCTACAGGA CCCAATGCCA CGTCTTCTCC ACCGAGCCCC CCGACCTCAG GGCCCAGTCC 
CAGGTCTCAG TGAGCGTGGA GGACTGTGAG GACACTCGGG AGGCAAAGGG GCCCTGGGGT 
GCACGGGTGG GCACGAGCGG TGGCAGCACC CACGGCCGGC ACACGGTGGG CAGCGACCCA 
GCCGAGCACC CGCTCCTGCG GCGCAAGAGC CTCCCGTGGG CGCGGAGGCT GAGCCGCAAG 
GGCTCCCGGC ACACGGGGAA AGCGGCAGCC GAGTGGATCA GCCAACAGCG GCTCAGCCTG 
TACCGGCGCT CGGAGCGGCA GGAGCTCTCA GAGCTGGTCA AGAACCGCAT GAAGCACCTG 
GGGCTGCCCA CCACTGGCTA CGACGAGATG AATGACCACC AGAACACCCT CTCCTATGTG 
CTCATCAACC CCCCGCCCGA CACGCGGCTG GAGCCCAATG ACATTGTATA TCTCATCCGC 
TCCGACCCCT TGGCCCATGT GGCCAGCAGC TCCCAGAGCA GGAAGAGCAG CTGCAGCAAC 
AAGCTGGCTT CCTGCAACCC CGAGACGCGG GACGAGACAC AGCTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	KCNT1
Entrez Gene ID	102407778
Full Name	potassium sodium-activated channel subfamily T member 1
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

KCNT1 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene

Clones

gRNAs

Target ORF information:

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