MTHFD2 cDNA ORF clone, Bubalus bubalis(water buffalo)

Gene
Clones
gRNAs

Summary

Gene Symbol	MTHFD2
Entrez Gene ID	102394714
Full Name	methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
Gene Type	protein-coding
Organism	Bubalus bubalis(water buffalo)

mRNA and Protein(s)

mRNA	Protein	Name
XM_006045423.1	XP_006045485.1	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
XM_006045423.2	XP_006045485.2	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial

Pathways from BioSystems
Gene Ontology

The following MTHFD2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MTHFD2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBu04457	XM_006045423.1 Latest version!	Bubalus bubalis methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
OBu45856	XM_006045423.2 Latest version!	Bubalus bubalis methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MTHFD2 ( XM_006045423.1 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006045485.1 Bubalus bubalis methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), mRNA.

Price & Availability↑

CloneID	OBu04457
Clone ID Related Accession (Same CDS sequence)	XM_006045423.1
Accession Version	XM_006045423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1137bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2014-03-11
Organism	Bubalus bubalis(water buffalo)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_005783722.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bubalus bubalis Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 5.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTGCCA GCAGGAGGCG AGGCCTGCCA CGCTGCAGCG GTATAACCGT GCGGCCAGCG 
CGCGCATCTT TCCCCGAGCT GACGATGGCT GCAGCCTCCT TTATAACCTC TTTGGTCACC 
CGACTGCTCC GGTCCGCGCA GAGTGGCCGC CTCCATCAAT GCCCTTTCCA CCTCTCGGCA 
GTGCGAAATG AAGCTGTTAT CATTTCTGGA AGGAAACTTG CTGAGCAGAT CAAGCAGGAA 
GTGCGGCAGG AGGTGGAAGA GTGGGTGGCG TCAGGCAACA AGCGGCCACA CCTGAGCGTG 
GTTCTGGTTG GCGAGAACCC TGCAAGTCAG TCCTATGTCC TCAACAAAAC CAGAGCAGCT 
GCCAGTGTGG GAATCAACAG TGAGACCATT CTGAAACCAG CTTCGATTTC AGAGGAAGAA 
CTGTTGAATT TAATCAATAA ACTAAATAAT GATGATAATG TAGACGGCCT CCTTGTTCAG 
CTGCCTCTTC CAGAGCACAT TGATGAGAGA AAGGTCTGCA ATGCTGTTTC TCCAGACAAA 
GATGTTGATG GCTTTCATGT AATTAATGTA GGGCGAATGT GTTTGGACCA GTGTTCCATG 
TTACCAGCTA CTCCATGGGG TGTGTGGGAA ATAATTAAGC GAACTGGCAT TCCAACCCTA 
GGGAAGAATG TGGTTGTGGC CGGAAGGTCA AAAAATGTTG GCATGCCCAT TGCAATGTTG 
CTGCACACAG ATGGGGCCCA TGAACGGCCC GGAGGCGATG CCACTGTCAC GATATCTCAT 
CGCTACACTC CTAAGGAGGA GCTAAAGAAA CACACAGCTC TTGCAGATAT TGTGATCTCC 
GCTGCAGGTA TTCCAAATCT GATCACAGCA GATATGATCA AGGAAGGAGC AGCTGTCATT 
GATGTGGGAA TAAATAGAAT TCAAGATCCC ATAACTGCTA AACCCAAGTT GGTTGGAGAT 
GTGGATTTTG AAGGAGTCAA GAAGAAAGCA GGTTACATCA CTCCAGTCCC TGGGGGTGTT 
GGTCCCATGA CTGTGGCAAT GCTGATGAAG AATACCATTA TTGCTGCAAA AAAAGTGCTG 
AGGCTCGAAG AACAGGAAGT ACTGAAGTCT AAGGAACTTG GAGTAGCAAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006045485.1
CDS	1..1137
Translation	MGASRRRGLPRCSGITVRPARASFPELTMAAASFITSLVTRLLRSAQSGRLHQCPFHLSAVRNEAVIISGRKLAEQIKQEVRQEVEEWVASGNKRPHLSVVLVGENPASQSYVLNKTRAAASVGINSETILKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERKVCNAVSPDKDVDGFHVINVGRMCLDQCSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEELKKHTALADIVISAAGIPNLITADMIKEGAAVIDVGINRIQDPITAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEQEVLKSKELGVASN

Target ORF information:

RefSeq Version	XM_006045423.1
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006045423.1

ORF Insert Sequence:

ATGGGTGCCA GCAGGAGGCG AGGCCTGCCA CGCTGCAGCG GTATAACCGT GCGGCCAGCG 
CGCGCATCTT TCCCCGAGCT GACGATGGCT GCAGCCTCCT TTATAACCTC TTTGGTCACC 
CGACTGCTCC GGTCCGCGCA GAGTGGCCGC CTCCATCAAT GCCCTTTCCA CCTCTCGGCA 
GTGCGAAATG AAGCTGTTAT CATTTCTGGA AGGAAACTTG CTGAGCAGAT CAAGCAGGAA 
GTGCGGCAGG AGGTGGAAGA GTGGGTGGCG TCAGGCAACA AGCGGCCACA CCTGAGCGTG 
GTTCTGGTTG GCGAGAACCC TGCAAGTCAG TCCTATGTCC TCAACAAAAC CAGAGCAGCT 
GCCAGTGTGG GAATCAACAG TGAGACCATT CTGAAACCAG CTTCGATTTC AGAGGAAGAA 
CTGTTGAATT TAATCAATAA ACTAAATAAT GATGATAATG TAGACGGCCT CCTTGTTCAG 
CTGCCTCTTC CAGAGCACAT TGATGAGAGA AAGGTCTGCA ATGCTGTTTC TCCAGACAAA 
GATGTTGATG GCTTTCATGT AATTAATGTA GGGCGAATGT GTTTGGACCA GTGTTCCATG 
TTACCAGCTA CTCCATGGGG TGTGTGGGAA ATAATTAAGC GAACTGGCAT TCCAACCCTA 
GGGAAGAATG TGGTTGTGGC CGGAAGGTCA AAAAATGTTG GCATGCCCAT TGCAATGTTG 
CTGCACACAG ATGGGGCCCA TGAACGGCCC GGAGGCGATG CCACTGTCAC GATATCTCAT 
CGCTACACTC CTAAGGAGGA GCTAAAGAAA CACACAGCTC TTGCAGATAT TGTGATCTCC 
GCTGCAGGTA TTCCAAATCT GATCACAGCA GATATGATCA AGGAAGGAGC AGCTGTCATT 
GATGTGGGAA TAAATAGAAT TCAAGATCCC ATAACTGCTA AACCCAAGTT GGTTGGAGAT 
GTGGATTTTG AAGGAGTCAA GAAGAAAGCA GGTTACATCA CTCCAGTCCC TGGGGGTGTT 
GGTCCCATGA CTGTGGCAAT GCTGATGAAG AATACCATTA TTGCTGCAAA AAAAGTGCTG 
AGGCTCGAAG AACAGGAAGT ACTGAAGTCT AAGGAACTTG GAGTAGCAAG TAATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MTHFD2 ( XM_006045423.2 ) cDNA ORF clone, Bubalus bubalis(water buffalo) -> XP_006045485.2 Bubalus bubalis methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), mRNA.

Price & Availability↑

CloneID	OBu45856
Clone ID Related Accession (Same CDS sequence)	XM_006045423.2
Accession Version	XM_006045423.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1053bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-23
Organism	Bubalus bubalis(water buffalo)
Product	bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037556.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2018 this sequence version replaced XM_006045423.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bubalus bubalis Annotation Release 101 Annotation Version :: 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCAG CCTCCTTTAT AACCTCTTTG GTCACCCGAC TGCTCCGGTC CGCGCAGAGT 
GGCCGCCTCC ATCAATGCCC TTTCCACCTC TCGGCAGTGC GAAATGAAGC TGTTATCATT 
TCTGGAAGGA AACTTGCTGA GCAGATCAAG CAGGAAGTGC GGCAGGAGGT GGAAGAGTGG 
GTGGCGTCAG GCAACAAGCG GCCACACCTG AGCGTGGTTC TGGTTGGCGA GAACCCTGCA 
AGTCAGTCCT ATGTCCTCAA CAAAACCAGA GCAGCTGCCA GTGTGGGAAT CAACAGTGAG 
ACCATTCTGA AACCAGCTTC GATTTCAGAG GAAGAACTGT TGAATTTAAT CAATAAACTA 
AATAATGATG ATAATGTAGA CGGCCTCCTT GTTCAGCTGC CTCTTCCAGA GCACATTGAT 
GAGAGAAAGG TCTGCAATGC TGTTTCTCCA GACAAAGATG TTGATGGCTT TCATGTAATT 
AATGTAGGGC GAATGTGTTT GGACCAGTGT TCCATGTTAC CAGCTACTCC ATGGGGTGTG 
TGGGAAATAA TTAAGCGAAC TGGCATTCCA ACCCTAGGGA AGAATGTGGT TGTGGCCGGA 
AGGTCAAAAA ATGTTGGCAT GCCCATTGCA ATGTTGCTGC ACACAGATGG GGCCCATGAA 
CGGCCCGGAG GCGATGCCAC TGTCACGATA TCTCATCGCT ACACTCCTAA GGAGGAGCTA 
AAGAAACACA CAGCTCTTGC AGATATTGTG ATCTCCGCTG CAGGTATTCC AAATCTGATC 
ACAGCAGATA TGATCAAGGA AGGAGCAGCT GTCATTGATG TGGGAATAAA TAGAATTCAA 
GATCCCATAA CTGCTAAACC CAAGTTGGTT GGAGATGTGG ATTTTGAAGG AGTCAAGAAG 
AAAGCAGGTT ACATCACTCC AGTCCCTGGG GGTGTTGGTC CCATGACTGT GGCAATGCTG 
ATGAAGAATA CCATTATTGC TGCAAAAAAA GTGCTGAGGC TCGAAGAACA GGAAGTACTG 
AAGTCTAAGG AACTTGGAGT AGCAAGTAAT TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006045485.2
CDS	54..1106
Translation	MAAASFITSLVTRLLRSAQSGRLHQCPFHLSAVRNEAVIISGRKLAEQIKQEVRQEVEEWVASGNKRPHLSVVLVGENPASQSYVLNKTRAAASVGINSETILKPASISEEELLNLINKLNNDDNVDGLLVQLPLPEHIDERKVCNAVSPDKDVDGFHVINVGRMCLDQCSMLPATPWGVWEIIKRTGIPTLGKNVVVAGRSKNVGMPIAMLLHTDGAHERPGGDATVTISHRYTPKEELKKHTALADIVISAAGIPNLITADMIKEGAAVIDVGINRIQDPITAKPKLVGDVDFEGVKKKAGYITPVPGGVGPMTVAMLMKNTIIAAKKVLRLEEQEVLKSKELGVASN

Target ORF information:

RefSeq Version	XM_006045423.2
Organism	Bubalus bubalis(water buffalo)
Definition	Bubalus bubalis methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase (MTHFD2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006045423.2

ORF Insert Sequence:

ATGGCTGCAG CCTCCTTTAT AACCTCTTTG GTCACCCGAC TGCTCCGGTC CGCGCAGAGT 
GGCCGCCTCC ATCAATGCCC TTTCCACCTC TCGGCAGTGC GAAATGAAGC TGTTATCATT 
TCTGGAAGGA AACTTGCTGA GCAGATCAAG CAGGAAGTGC GGCAGGAGGT GGAAGAGTGG 
GTGGCGTCAG GCAACAAGCG GCCACACCTG AGCGTGGTTC TGGTTGGCGA GAACCCTGCA 
AGTCAGTCCT ATGTCCTCAA CAAAACCAGA GCAGCTGCCA GTGTGGGAAT CAACAGTGAG 
ACCATTCTGA AACCAGCTTC GATTTCAGAG GAAGAACTGT TGAATTTAAT CAATAAACTA 
AATAATGATG ATAATGTAGA CGGCCTCCTT GTTCAGCTGC CTCTTCCAGA GCACATTGAT 
GAGAGAAAGG TCTGCAATGC TGTTTCTCCA GACAAAGATG TTGATGGCTT TCATGTAATT 
AATGTAGGGC GAATGTGTTT GGACCAGTGT TCCATGTTAC CAGCTACTCC ATGGGGTGTG 
TGGGAAATAA TTAAGCGAAC TGGCATTCCA ACCCTAGGGA AGAATGTGGT TGTGGCCGGA 
AGGTCAAAAA ATGTTGGCAT GCCCATTGCA ATGTTGCTGC ACACAGATGG GGCCCATGAA 
CGGCCCGGAG GCGATGCCAC TGTCACGATA TCTCATCGCT ACACTCCTAA GGAGGAGCTA 
AAGAAACACA CAGCTCTTGC AGATATTGTG ATCTCCGCTG CAGGTATTCC AAATCTGATC 
ACAGCAGATA TGATCAAGGA AGGAGCAGCT GTCATTGATG TGGGAATAAA TAGAATTCAA 
GATCCCATAA CTGCTAAACC CAAGTTGGTT GGAGATGTGG ATTTTGAAGG AGTCAAGAAG 
AAAGCAGGTT ACATCACTCC AGTCCCTGGG GGTGTTGGTC CCATGACTGT GGCAATGCTG 
ATGAAGAATA CCATTATTGC TGCAAAAAAA GTGCTGAGGC TCGAAGAACA GGAAGTACTG 
AAGTCTAAGG AACTTGGAGT AGCAAGTAAT TAA