prune2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	prune2
Entrez Gene ID	780047
Full Name	prune homolog 2
Synonyms	bmcc1,bnipxl
General protein information	Preferred Names prune homolog 2 Names protein prune homolog 2 BNIP2 motif-containing molecule at the C-terminal region 1
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_031901874.1	XP_031757734.1	protein prune homolog 2 isoform X5
XM_031901877.1	XP_031757737.1	protein prune homolog 2 isoform X6
XM_031901857.1	XP_031757717.1	protein prune homolog 2 isoform X2
NM_001079124.2	NP_001072592.1	protein prune homolog 2
XM_031901864.1	XP_031757724.1	protein prune homolog 2 isoform X3
XM_031901872.1	XP_031757732.1	protein prune homolog 2 isoform X4
NM_001079124.1	NP_001072592.1	protein prune homolog 2
XM_031901853.1	XP_031757713.1	protein prune homolog 2 isoform X1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	prune2	NP_001104624.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP005565	XP_315572.4
Xenopus tropicalis (tropical clawed frog)	prune2	NP_001072592.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following prune2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the prune2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa24760	NM_001079124.2 Latest version!	Xenopus tropicalis prune homolog 2 (prune2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa48045	XM_031901877.1 Latest version!	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
OXa48040	XM_031901853.1 Latest version!	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa48041	XM_031901857.1 Latest version!	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa48042	XM_031901864.1 Latest version!	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa48043	XM_031901872.1 Latest version!	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1301.30 $1859.00
OXa48044	XM_031901874.1 Latest version!	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1091.30 $1559.00
View Old Accession Versions >>
OXa24760	NM_001079124.1	Xenopus tropicalis prune homolog 2 (prune2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

prune2 ( NM_001079124.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072592.1 Xenopus tropicalis prune homolog 2 (prune2), mRNA.

Price & Availability↑

CloneID	OXa24760
Clone ID Related Accession (Same CDS sequence)	NM_001079124.2 , NM_001079124.1
Accession Version	NM_001079124.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-31
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC122960.1. On May 2, 2019 this sequence version replaced NM_001079124.1. ##Evidence-Data-START## Transcript exon combination :: BC122960.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01758126 [ECO:0000348] ##Evidence-Data-END##

ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC 
CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT 
AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT 
GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA 
GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG 
CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC 
TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC 
CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG 
CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA 
ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG 
AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG 
ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA 
GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG 
GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA 
ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072592.1
CDS	120..1070
Translation	MDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILALTRPFISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKLDEELRESESPKAGCLPNEPEMNTLEEEFENKMGDND

Target ORF information:

RefSeq Version	NM_001079124.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079124.2

ORF Insert Sequence:

ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC 
CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT 
AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT 
GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA 
GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG 
CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC 
TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC 
CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG 
CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA 
ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG 
AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG 
ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA 
GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG 
GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA 
ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prune2 ( NM_001079124.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001072592.1 Xenopus tropicalis prune homolog 2 (prune2), mRNA.

Price & Availability↑

CloneID	OXa24760
Clone ID Related Accession (Same CDS sequence)	NM_001079124.2 , NM_001079124.1
Accession Version	NM_001079124.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 951bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-10-29
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC122960.1. ##Evidence-Data-START## Transcript exon combination :: BC122960.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00861994, SAMN00861995 [ECO:0000350] ##Evidence-Data-END##

ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC 
CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT 
AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT 
GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA 
GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG 
CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC 
TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC 
CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG 
CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA 
ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG 
AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG 
ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA 
GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG 
GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA 
ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001072592.1
CDS	120..1070
Translation	MDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILALTRPFISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKLDEELRESESPKAGCLPNEPEMNTLEEEFENKMGDND

Target ORF information:

RefSeq Version	NM_001079124.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001079124.1

ORF Insert Sequence:

ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC 
CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT 
AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT 
GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA 
GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG 
CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC 
TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC 
CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG 
CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA 
ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG 
AATTTAAAGT CTTTTATAAT TGTTCATCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG 
ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA 
GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAACTG 
GATGAAGAAT TGAGAGAAAG TGAATCTCCC AAAGCTGGCT GTCTTCCCAA TGAACCTGAA 
ATGAATACCT TGGAGGAGGA ATTTGAAAAC AAGATGGGAG ACAACGACTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prune2 ( XM_031901877.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757737.1 Xenopus tropicalis prune homolog 2 (prune2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OXa48045
Clone ID Related Accession (Same CDS sequence)	XM_031901877.1
Accession Version	XM_031901877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 987bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC 
CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT 
AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT 
GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA 
GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG 
CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC 
TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC 
CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG 
CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA 
ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG 
AATTTAAAGT CTTTTATAAT TGTTCACCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG 
ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA 
GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAGTAT 
GAAGAATCAA GATGTTTTCC AAGAACTGTG AGACTGGATG AAGAATTGAG AGAAAGTGAA 
TCTCCCAAAG CTGGCTGTCT TCCCAATGAA CCTGAAATGA ATACCTTGGA GGAGGAATTT 
GAAAACAAGA TGGGAGACAA CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757737.1
CDS	191..1177
Translation	MDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILALTRPFISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKYEESRCFPRTVRLDEELRESESPKAGCLPNEPEMNTLEEEFENKMGDNA

Target ORF information:

RefSeq Version	XM_031901877.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901877.1

ORF Insert Sequence:

ATGGACATTC CTCTTGATGC AGCAGAAATA CGACCTGAAC CTCCAAATTC CTTGGATTTA 
AATGGCTCCA GCACCAGAAA AATAAAGCTT ACTGCTCCCA ATATCAACCT ATCTCTGGAC 
CACAGTGAAG GATCAATCCT ATCTGATGAC AATCTTGATA CCCCAGATGA GCTTGACATT 
AATGTTGATG ATCTTGACAC TCCAGATGAA GCAGACTCCT TTGACTACAC AGGCCAGGAT 
GACCAACCTG CACTTGGGGA AGCCGTACAG GAGGATTTTG AATCTATACA AGAGTACACA 
GCAGAAGAGG AAAGAGCTGA TAACAGACTA TGGAGGACAG TTGTCATTGG AGAGCAAGAG 
CAGAGGATTG ATATGAAGGT CATTGAACCA TACAAAAAAG TTATATCTCA TGGAGGTTAC 
TATGGTGAAG GTGTAAATGC CATCATTGTG TTCGCTGCAT GCTTTTTGCC TGATAGCAGC 
CGTCCAGACT ACAATTATGT CATGGAAAAC CTTTTCCTCT ATGTGATAAG CACATTGGAG 
CTTATGGTGG CAGAAGACTA TATGGTTGTC TATTTGAATG GTGCTACACC AAGAAGAAAA 
ATGCCTGGTT TAGGATGGAT GAAGAAATGC TACCAGATGA TTGATCGGCG GTTACGGAAG 
AATTTAAAGT CTTTTATAAT TGTTCACCCA TCGTGGTTTA TCAGGACAAT ATTAGCACTG 
ACAAGACCTT TTATCAGCTC CAAATTTAGC AGCAAGATAA AGTATGTGTC CACTTTAGCA 
GAGCTCAGCG AATTGATTCC AATGGAATAT GTGCACATTC CAGAGACTAT TGTGAAGTAT 
GAAGAATCAA GATGTTTTCC AAGAACTGTG AGACTGGATG AAGAATTGAG AGAAAGTGAA 
TCTCCCAAAG CTGGCTGTCT TCCCAATGAA CCTGAAATGA ATACCTTGGA GGAGGAATTT 
GAAAACAAGA TGGGAGACAA CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prune2 ( XM_031901853.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757713.1 Xenopus tropicalis prune homolog 2 (prune2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa48040
Clone ID Related Accession (Same CDS sequence)	XM_031901853.1
Accession Version	XM_031901853.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7155bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA 
TGTTTTCCAA GAACTGTGAG ACTGGATGAA GAATTGAGAG AAAGTGAATC TCCCAAAGCT 
GGCTGTCTTC CCAATGAACC TGAAATGAAT ACCTTGGAGG AGGAATTTGA AAACAAGATG 
GGAGACAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757713.1
CDS	12..7166
Translation	MSCRNIIGELKLFLDKYGIDILILLASYTTEEQMAVQQIAVHSENPELCNQVCCELEECQNPFLDLEPSDYGCEQFLVYHQENSSVTCEQVAAVIKEAINRRRLGMMPNSRTSSTEAVAGSAPLSQGSSGIMELYGSDLDPQPNPVNFVDNVQDGNAQDINAQAQVDVNVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVADAAGSHHNFLPGIDNYSRVPEGVIVEEETPPSRTNSDNAELFNFDLLPNTRSESSSHSADYSMADDFFFQSDSSEGQQVQKQQLYRNDIAHCSTNLLNTKVPNISLVDFDDAFMHSPENHEDLCEKTPSASDITEFESSLSSEILGSADVKVPPTPMNSLVESSPLDNGPPTFFPEDVVQKINEIGFSVSETQAYWWNESENIDANTAPMNADAWSSSNQSVLHRPESWKEQISGQDNANARHAPSFQVPCPGLHNDNSGNHDKNTRQENILIPDLWNFNNPLQGKSDPFSGTSDKDEFGDYVKKSEDKLCAFKEDSKTKYFAKRKAKSNIDTDISFENTAELHEESRYSQEFLDESQFMLQQRSTEDLGVDAKTLLPGHIERNLGMWDFLDAGQDTNIVDHHINWEDPFLSCGPYRCLDFTASYKGKDCIVSPPDTNYSTSESNLSPASEDDMKEPEKSDDQEKHLLNEGHQSSNDLNILNNRELASNNRHAVSENKNVRNHSDVSIQMMPKRSCFGNYLHSDYLDNAIHSSTPSKSKNNEPNYEMNNHKHDLSILSPNRSTLLEASSHNLLSKEEAACFNSERAHKVPDSFLPLPIHNNTVFNILPQQGQSEINECTRNSYPSNEVPNEAHGKGQGQSTLEIMNKHDNKVKSGQDHHLPISKPQHNPFYESLSPDIKDKVLNPLENSGKCQDICVLQEKGGELGSAGYTNQDNWENDLQEDTESSSLDSPDDLESSDSSYLRSWNLLVKSPVLKDDLRIPGQLSSVHEQDDYEIKSPGVKSKSYNEKSILLSPEGKVNDMNDAQISSENDHLTLNKVKSKENVFLNDIAITKKTDISHSEQEGSVIQGEGSTDKWNKTKLCSYESAGINSELFPHMSNVYVKKKINIYAYNEEDVQSNSSLSGETSPDLDFSWNRLEEKGYNSTSSVYGSGSKNNQPFSFIQNESANNKCLNPQGKVQLNTVNKVPMNLDIWNTQICEDSESSSSPEGNETRNHSNSSDEKALNYYQNIHTSLDNADSNSTTPFTDEESPEIEDILGSFGFIQDGCVNNVMKEEKEQKVSLTKSNCKAACEEGMLNQDCLQHVQGSPEDRSQSYENAHQDHDKHYREIFIKEETFHLGADTKDAFTRGSQSAETLDLGHTHNKVQSPEDTYFTISPQLSHSYHENGDDKRKECSDVVGTCSSLNVSPLSLCYEDNYSNTENYEEDSSVKRQSLNNLKTNPRQTKSTNSAPLISPMDNINVKEFKPVSCHDKSFNKTEPFVQLQAEMKNVNNTIKDTISEPRTQADLSREKNLQGTVGALGQISRHPNMAPHHSEEDSLLFNGQVEQVDLSNQIQQYGGSEISDSMLFECIPDILDDGPREFSPFYSISPDLWSISEHVYIRKSEYGSPDVLNSCDPNSHVSKTPDLYKQCGRPYKDVFVWPDTAHSSLSPRGSAELSQISTEGKHNETYMQNAHLHKNNSILQPCMKCTESVSSCSNMEHVCEKHSVDHYNIGCSQVNAPSSAISVKGINEHVLLDRSASANLILQECMPYTQENEENIQRDVFQTTKTHAEPFINDNSAAEVLNPCLHQGKVPGNANEENWNNVTSPADGDVGPHHENGEESDRVWNADNMTRRSLVLFDKGRDSIKLQTEDPVSSNADEEVHGHGPCESVNRSVMTVTNVAFQQTKSNERHSVSSPDTFQSISMTNVNEKQAIHRPECHSLYIEEKSSPIVSRRLGSEKKSEEFSEQEHSWSIILSQNEASDSSPEDIFSREETVDTGKGLEDTFQLGYSCDKQGGYQIDTGGQDYTELEESFEMCKVEERDIVAHVSHDLLNLQSISVHIQLPPQDGELAKYLGYKEKTENGSILDDEGMDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILALTRPFISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKYEESRCFPRTVRLDEELRESESPKAGCLPNEPEMNTLEEEFENKMGDNA

Target ORF information:

RefSeq Version	XM_031901853.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901853.1

ORF Insert Sequence:

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA 
TGTTTTCCAA GAACTGTGAG ACTGGATGAA GAATTGAGAG AAAGTGAATC TCCCAAAGCT 
GGCTGTCTTC CCAATGAACC TGAAATGAAT ACCTTGGAGG AGGAATTTGA AAACAAGATG 
GGAGACAACG CCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prune2 ( XM_031901857.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757717.1 Xenopus tropicalis prune homolog 2 (prune2), transcript variant X2, mRNA.

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CloneID	OXa48041
Clone ID Related Accession (Same CDS sequence)	XM_031901857.1
Accession Version	XM_031901857.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAACTGGA TGAAGAATTG 
AGAGAAAGTG AATCTCCCAA AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG 
GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757717.1
CDS	12..7130
Translation	MSCRNIIGELKLFLDKYGIDILILLASYTTEEQMAVQQIAVHSENPELCNQVCCELEECQNPFLDLEPSDYGCEQFLVYHQENSSVTCEQVAAVIKEAINRRRLGMMPNSRTSSTEAVAGSAPLSQGSSGIMELYGSDLDPQPNPVNFVDNVQDGNAQDINAQAQVDVNVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVADAAGSHHNFLPGIDNYSRVPEGVIVEEETPPSRTNSDNAELFNFDLLPNTRSESSSHSADYSMADDFFFQSDSSEGQQVQKQQLYRNDIAHCSTNLLNTKVPNISLVDFDDAFMHSPENHEDLCEKTPSASDITEFESSLSSEILGSADVKVPPTPMNSLVESSPLDNGPPTFFPEDVVQKINEIGFSVSETQAYWWNESENIDANTAPMNADAWSSSNQSVLHRPESWKEQISGQDNANARHAPSFQVPCPGLHNDNSGNHDKNTRQENILIPDLWNFNNPLQGKSDPFSGTSDKDEFGDYVKKSEDKLCAFKEDSKTKYFAKRKAKSNIDTDISFENTAELHEESRYSQEFLDESQFMLQQRSTEDLGVDAKTLLPGHIERNLGMWDFLDAGQDTNIVDHHINWEDPFLSCGPYRCLDFTASYKGKDCIVSPPDTNYSTSESNLSPASEDDMKEPEKSDDQEKHLLNEGHQSSNDLNILNNRELASNNRHAVSENKNVRNHSDVSIQMMPKRSCFGNYLHSDYLDNAIHSSTPSKSKNNEPNYEMNNHKHDLSILSPNRSTLLEASSHNLLSKEEAACFNSERAHKVPDSFLPLPIHNNTVFNILPQQGQSEINECTRNSYPSNEVPNEAHGKGQGQSTLEIMNKHDNKVKSGQDHHLPISKPQHNPFYESLSPDIKDKVLNPLENSGKCQDICVLQEKGGELGSAGYTNQDNWENDLQEDTESSSLDSPDDLESSDSSYLRSWNLLVKSPVLKDDLRIPGQLSSVHEQDDYEIKSPGVKSKSYNEKSILLSPEGKVNDMNDAQISSENDHLTLNKVKSKENVFLNDIAITKKTDISHSEQEGSVIQGEGSTDKWNKTKLCSYESAGINSELFPHMSNVYVKKKINIYAYNEEDVQSNSSLSGETSPDLDFSWNRLEEKGYNSTSSVYGSGSKNNQPFSFIQNESANNKCLNPQGKVQLNTVNKVPMNLDIWNTQICEDSESSSSPEGNETRNHSNSSDEKALNYYQNIHTSLDNADSNSTTPFTDEESPEIEDILGSFGFIQDGCVNNVMKEEKEQKVSLTKSNCKAACEEGMLNQDCLQHVQGSPEDRSQSYENAHQDHDKHYREIFIKEETFHLGADTKDAFTRGSQSAETLDLGHTHNKVQSPEDTYFTISPQLSHSYHENGDDKRKECSDVVGTCSSLNVSPLSLCYEDNYSNTENYEEDSSVKRQSLNNLKTNPRQTKSTNSAPLISPMDNINVKEFKPVSCHDKSFNKTEPFVQLQAEMKNVNNTIKDTISEPRTQADLSREKNLQGTVGALGQISRHPNMAPHHSEEDSLLFNGQVEQVDLSNQIQQYGGSEISDSMLFECIPDILDDGPREFSPFYSISPDLWSISEHVYIRKSEYGSPDVLNSCDPNSHVSKTPDLYKQCGRPYKDVFVWPDTAHSSLSPRGSAELSQISTEGKHNETYMQNAHLHKNNSILQPCMKCTESVSSCSNMEHVCEKHSVDHYNIGCSQVNAPSSAISVKGINEHVLLDRSASANLILQECMPYTQENEENIQRDVFQTTKTHAEPFINDNSAAEVLNPCLHQGKVPGNANEENWNNVTSPADGDVGPHHENGEESDRVWNADNMTRRSLVLFDKGRDSIKLQTEDPVSSNADEEVHGHGPCESVNRSVMTVTNVAFQQTKSNERHSVSSPDTFQSISMTNVNEKQAIHRPECHSLYIEEKSSPIVSRRLGSEKKSEEFSEQEHSWSIILSQNEASDSSPEDIFSREETVDTGKGLEDTFQLGYSCDKQGGYQIDTGGQDYTELEESFEMCKVEERDIVAHVSHDLLNLQSISVHIQLPPQDGELAKYLGYKEKTENGSILDDEGMDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILALTRPFISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKLDEELRESESPKAGCLPNEPEMNTLEEEFENKMGDNA

Target ORF information:

RefSeq Version	XM_031901857.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901857.1

ORF Insert Sequence:

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAACTGGA TGAAGAATTG 
AGAGAAAGTG AATCTCCCAA AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG 
GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prune2 ( XM_031901864.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757724.1 Xenopus tropicalis prune homolog 2 (prune2), transcript variant X3, mRNA.

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CloneID	OXa48042
Clone ID Related Accession (Same CDS sequence)	XM_031901864.1
Accession Version	XM_031901864.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7119bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA 
TGTTTTCCAA GAACTGTGAG AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG 
GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757724.1
CDS	12..7130
Translation	MSCRNIIGELKLFLDKYGIDILILLASYTTEEQMAVQQIAVHSENPELCNQVCCELEECQNPFLDLEPSDYGCEQFLVYHQENSSVTCEQVAAVIKEAINRRRLGMMPNSRTSSTEAVAGSAPLSQGSSGIMELYGSDLDPQPNPVNFVDNVQDGNAQDINAQAQVDVNVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVADAAGSHHNFLPGIDNYSRVPEGVIVEEETPPSRTNSDNAELFNFDLLPNTRSESSSHSADYSMADDFFFQSDSSEGQQVQKQQLYRNDIAHCSTNLLNTKVPNISLVDFDDAFMHSPENHEDLCEKTPSASDITEFESSLSSEILGSADVKVPPTPMNSLVESSPLDNGPPTFFPEDVVQKINEIGFSVSETQAYWWNESENIDANTAPMNADAWSSSNQSVLHRPESWKEQISGQDNANARHAPSFQVPCPGLHNDNSGNHDKNTRQENILIPDLWNFNNPLQGKSDPFSGTSDKDEFGDYVKKSEDKLCAFKEDSKTKYFAKRKAKSNIDTDISFENTAELHEESRYSQEFLDESQFMLQQRSTEDLGVDAKTLLPGHIERNLGMWDFLDAGQDTNIVDHHINWEDPFLSCGPYRCLDFTASYKGKDCIVSPPDTNYSTSESNLSPASEDDMKEPEKSDDQEKHLLNEGHQSSNDLNILNNRELASNNRHAVSENKNVRNHSDVSIQMMPKRSCFGNYLHSDYLDNAIHSSTPSKSKNNEPNYEMNNHKHDLSILSPNRSTLLEASSHNLLSKEEAACFNSERAHKVPDSFLPLPIHNNTVFNILPQQGQSEINECTRNSYPSNEVPNEAHGKGQGQSTLEIMNKHDNKVKSGQDHHLPISKPQHNPFYESLSPDIKDKVLNPLENSGKCQDICVLQEKGGELGSAGYTNQDNWENDLQEDTESSSLDSPDDLESSDSSYLRSWNLLVKSPVLKDDLRIPGQLSSVHEQDDYEIKSPGVKSKSYNEKSILLSPEGKVNDMNDAQISSENDHLTLNKVKSKENVFLNDIAITKKTDISHSEQEGSVIQGEGSTDKWNKTKLCSYESAGINSELFPHMSNVYVKKKINIYAYNEEDVQSNSSLSGETSPDLDFSWNRLEEKGYNSTSSVYGSGSKNNQPFSFIQNESANNKCLNPQGKVQLNTVNKVPMNLDIWNTQICEDSESSSSPEGNETRNHSNSSDEKALNYYQNIHTSLDNADSNSTTPFTDEESPEIEDILGSFGFIQDGCVNNVMKEEKEQKVSLTKSNCKAACEEGMLNQDCLQHVQGSPEDRSQSYENAHQDHDKHYREIFIKEETFHLGADTKDAFTRGSQSAETLDLGHTHNKVQSPEDTYFTISPQLSHSYHENGDDKRKECSDVVGTCSSLNVSPLSLCYEDNYSNTENYEEDSSVKRQSLNNLKTNPRQTKSTNSAPLISPMDNINVKEFKPVSCHDKSFNKTEPFVQLQAEMKNVNNTIKDTISEPRTQADLSREKNLQGTVGALGQISRHPNMAPHHSEEDSLLFNGQVEQVDLSNQIQQYGGSEISDSMLFECIPDILDDGPREFSPFYSISPDLWSISEHVYIRKSEYGSPDVLNSCDPNSHVSKTPDLYKQCGRPYKDVFVWPDTAHSSLSPRGSAELSQISTEGKHNETYMQNAHLHKNNSILQPCMKCTESVSSCSNMEHVCEKHSVDHYNIGCSQVNAPSSAISVKGINEHVLLDRSASANLILQECMPYTQENEENIQRDVFQTTKTHAEPFINDNSAAEVLNPCLHQGKVPGNANEENWNNVTSPADGDVGPHHENGEESDRVWNADNMTRRSLVLFDKGRDSIKLQTEDPVSSNADEEVHGHGPCESVNRSVMTVTNVAFQQTKSNERHSVSSPDTFQSISMTNVNEKQAIHRPECHSLYIEEKSSPIVSRRLGSEKKSEEFSEQEHSWSIILSQNEASDSSPEDIFSREETVDTGKGLEDTFQLGYSCDKQGGYQIDTGGQDYTELEESFEMCKVEERDIVAHVSHDLLNLQSISVHIQLPPQDGELAKYLGYKEKTENGSILDDEGMDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILALTRPFISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKYEESRCFPRTVRAGCLPNEPEMNTLEEEFENKMGDNA

Target ORF information:

RefSeq Version	XM_031901864.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901864.1

ORF Insert Sequence:

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAGTATGA AGAATCAAGA 
TGTTTTCCAA GAACTGTGAG AGCTGGCTGT CTTCCCAATG AACCTGAAAT GAATACCTTG 
GAGGAGGAAT TTGAAAACAA GATGGGAGAC AACGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prune2 ( XM_031901872.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757732.1 Xenopus tropicalis prune homolog 2 (prune2), transcript variant X4, mRNA.

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CloneID	OXa48043
Clone ID Related Accession (Same CDS sequence)	XM_031901872.1
Accession Version	XM_031901872.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7083bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAAGCTGG CTGTCTTCCC 
AATGAACCTG AAATGAATAC CTTGGAGGAG GAATTTGAAA ACAAGATGGG AGACAACGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757732.1
CDS	12..7094
Translation	MSCRNIIGELKLFLDKYGIDILILLASYTTEEQMAVQQIAVHSENPELCNQVCCELEECQNPFLDLEPSDYGCEQFLVYHQENSSVTCEQVAAVIKEAINRRRLGMMPNSRTSSTEAVAGSAPLSQGSSGIMELYGSDLDPQPNPVNFVDNVQDGNAQDINAQAQVDVNVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVADAAGSHHNFLPGIDNYSRVPEGVIVEEETPPSRTNSDNAELFNFDLLPNTRSESSSHSADYSMADDFFFQSDSSEGQQVQKQQLYRNDIAHCSTNLLNTKVPNISLVDFDDAFMHSPENHEDLCEKTPSASDITEFESSLSSEILGSADVKVPPTPMNSLVESSPLDNGPPTFFPEDVVQKINEIGFSVSETQAYWWNESENIDANTAPMNADAWSSSNQSVLHRPESWKEQISGQDNANARHAPSFQVPCPGLHNDNSGNHDKNTRQENILIPDLWNFNNPLQGKSDPFSGTSDKDEFGDYVKKSEDKLCAFKEDSKTKYFAKRKAKSNIDTDISFENTAELHEESRYSQEFLDESQFMLQQRSTEDLGVDAKTLLPGHIERNLGMWDFLDAGQDTNIVDHHINWEDPFLSCGPYRCLDFTASYKGKDCIVSPPDTNYSTSESNLSPASEDDMKEPEKSDDQEKHLLNEGHQSSNDLNILNNRELASNNRHAVSENKNVRNHSDVSIQMMPKRSCFGNYLHSDYLDNAIHSSTPSKSKNNEPNYEMNNHKHDLSILSPNRSTLLEASSHNLLSKEEAACFNSERAHKVPDSFLPLPIHNNTVFNILPQQGQSEINECTRNSYPSNEVPNEAHGKGQGQSTLEIMNKHDNKVKSGQDHHLPISKPQHNPFYESLSPDIKDKVLNPLENSGKCQDICVLQEKGGELGSAGYTNQDNWENDLQEDTESSSLDSPDDLESSDSSYLRSWNLLVKSPVLKDDLRIPGQLSSVHEQDDYEIKSPGVKSKSYNEKSILLSPEGKVNDMNDAQISSENDHLTLNKVKSKENVFLNDIAITKKTDISHSEQEGSVIQGEGSTDKWNKTKLCSYESAGINSELFPHMSNVYVKKKINIYAYNEEDVQSNSSLSGETSPDLDFSWNRLEEKGYNSTSSVYGSGSKNNQPFSFIQNESANNKCLNPQGKVQLNTVNKVPMNLDIWNTQICEDSESSSSPEGNETRNHSNSSDEKALNYYQNIHTSLDNADSNSTTPFTDEESPEIEDILGSFGFIQDGCVNNVMKEEKEQKVSLTKSNCKAACEEGMLNQDCLQHVQGSPEDRSQSYENAHQDHDKHYREIFIKEETFHLGADTKDAFTRGSQSAETLDLGHTHNKVQSPEDTYFTISPQLSHSYHENGDDKRKECSDVVGTCSSLNVSPLSLCYEDNYSNTENYEEDSSVKRQSLNNLKTNPRQTKSTNSAPLISPMDNINVKEFKPVSCHDKSFNKTEPFVQLQAEMKNVNNTIKDTISEPRTQADLSREKNLQGTVGALGQISRHPNMAPHHSEEDSLLFNGQVEQVDLSNQIQQYGGSEISDSMLFECIPDILDDGPREFSPFYSISPDLWSISEHVYIRKSEYGSPDVLNSCDPNSHVSKTPDLYKQCGRPYKDVFVWPDTAHSSLSPRGSAELSQISTEGKHNETYMQNAHLHKNNSILQPCMKCTESVSSCSNMEHVCEKHSVDHYNIGCSQVNAPSSAISVKGINEHVLLDRSASANLILQECMPYTQENEENIQRDVFQTTKTHAEPFINDNSAAEVLNPCLHQGKVPGNANEENWNNVTSPADGDVGPHHENGEESDRVWNADNMTRRSLVLFDKGRDSIKLQTEDPVSSNADEEVHGHGPCESVNRSVMTVTNVAFQQTKSNERHSVSSPDTFQSISMTNVNEKQAIHRPECHSLYIEEKSSPIVSRRLGSEKKSEEFSEQEHSWSIILSQNEASDSSPEDIFSREETVDTGKGLEDTFQLGYSCDKQGGYQIDTGGQDYTELEESFEMCKVEERDIVAHVSHDLLNLQSISVHIQLPPQDGELAKYLGYKEKTENGSILDDEGMDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRLRKNLKSFIIVHPSWFIRTILALTRPFISSKFSSKIKYVSTLAELSELIPMEYVHIPETIVKAGCLPNEPEMNTLEEEFENKMGDNA

Target ORF information:

RefSeq Version	XM_031901872.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901872.1

ORF Insert Sequence:

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGGT TACGGAAGAA TTTAAAGTCT 
TTTATAATTG TTCACCCATC GTGGTTTATC AGGACAATAT TAGCACTGAC AAGACCTTTT 
ATCAGCTCCA AATTTAGCAG CAAGATAAAG TATGTGTCCA CTTTAGCAGA GCTCAGCGAA 
TTGATTCCAA TGGAATATGT GCACATTCCA GAGACTATTG TGAAAGCTGG CTGTCTTCCC 
AATGAACCTG AAATGAATAC CTTGGAGGAG GAATTTGAAA ACAAGATGGG AGACAACGCC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

prune2 ( XM_031901874.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_031757734.1 Xenopus tropicalis prune homolog 2 (prune2), transcript variant X5, mRNA.

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CloneID	OXa48044
Clone ID Related Accession (Same CDS sequence)	XM_031901874.1
Accession Version	XM_031901874.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6897bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	protein prune homolog 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGAG CTGGCTGTCT TCCCAATGAA 
CCTGAAATGA ATACCTTGGA GGAGGAATTT GAAAACAAGA TGGGAGACAA CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031757734.1
CDS	12..6908
Translation	MSCRNIIGELKLFLDKYGIDILILLASYTTEEQMAVQQIAVHSENPELCNQVCCELEECQNPFLDLEPSDYGCEQFLVYHQENSSVTCEQVAAVIKEAINRRRLGMMPNSRTSSTEAVAGSAPLSQGSSGIMELYGSDLDPQPNPVNFVDNVQDGNAQDINAQAQVDVNVDLVSPDSGLATIRSSRSSKESSVFLSDDSPVADAAGSHHNFLPGIDNYSRVPEGVIVEEETPPSRTNSDNAELFNFDLLPNTRSESSSHSADYSMADDFFFQSDSSEGQQVQKQQLYRNDIAHCSTNLLNTKVPNISLVDFDDAFMHSPENHEDLCEKTPSASDITEFESSLSSEILGSADVKVPPTPMNSLVESSPLDNGPPTFFPEDVVQKINEIGFSVSETQAYWWNESENIDANTAPMNADAWSSSNQSVLHRPESWKEQISGQDNANARHAPSFQVPCPGLHNDNSGNHDKNTRQENILIPDLWNFNNPLQGKSDPFSGTSDKDEFGDYVKKSEDKLCAFKEDSKTKYFAKRKAKSNIDTDISFENTAELHEESRYSQEFLDESQFMLQQRSTEDLGVDAKTLLPGHIERNLGMWDFLDAGQDTNIVDHHINWEDPFLSCGPYRCLDFTASYKGKDCIVSPPDTNYSTSESNLSPASEDDMKEPEKSDDQEKHLLNEGHQSSNDLNILNNRELASNNRHAVSENKNVRNHSDVSIQMMPKRSCFGNYLHSDYLDNAIHSSTPSKSKNNEPNYEMNNHKHDLSILSPNRSTLLEASSHNLLSKEEAACFNSERAHKVPDSFLPLPIHNNTVFNILPQQGQSEINECTRNSYPSNEVPNEAHGKGQGQSTLEIMNKHDNKVKSGQDHHLPISKPQHNPFYESLSPDIKDKVLNPLENSGKCQDICVLQEKGGELGSAGYTNQDNWENDLQEDTESSSLDSPDDLESSDSSYLRSWNLLVKSPVLKDDLRIPGQLSSVHEQDDYEIKSPGVKSKSYNEKSILLSPEGKVNDMNDAQISSENDHLTLNKVKSKENVFLNDIAITKKTDISHSEQEGSVIQGEGSTDKWNKTKLCSYESAGINSELFPHMSNVYVKKKINIYAYNEEDVQSNSSLSGETSPDLDFSWNRLEEKGYNSTSSVYGSGSKNNQPFSFIQNESANNKCLNPQGKVQLNTVNKVPMNLDIWNTQICEDSESSSSPEGNETRNHSNSSDEKALNYYQNIHTSLDNADSNSTTPFTDEESPEIEDILGSFGFIQDGCVNNVMKEEKEQKVSLTKSNCKAACEEGMLNQDCLQHVQGSPEDRSQSYENAHQDHDKHYREIFIKEETFHLGADTKDAFTRGSQSAETLDLGHTHNKVQSPEDTYFTISPQLSHSYHENGDDKRKECSDVVGTCSSLNVSPLSLCYEDNYSNTENYEEDSSVKRQSLNNLKTNPRQTKSTNSAPLISPMDNINVKEFKPVSCHDKSFNKTEPFVQLQAEMKNVNNTIKDTISEPRTQADLSREKNLQGTVGALGQISRHPNMAPHHSEEDSLLFNGQVEQVDLSNQIQQYGGSEISDSMLFECIPDILDDGPREFSPFYSISPDLWSISEHVYIRKSEYGSPDVLNSCDPNSHVSKTPDLYKQCGRPYKDVFVWPDTAHSSLSPRGSAELSQISTEGKHNETYMQNAHLHKNNSILQPCMKCTESVSSCSNMEHVCEKHSVDHYNIGCSQVNAPSSAISVKGINEHVLLDRSASANLILQECMPYTQENEENIQRDVFQTTKTHAEPFINDNSAAEVLNPCLHQGKVPGNANEENWNNVTSPADGDVGPHHENGEESDRVWNADNMTRRSLVLFDKGRDSIKLQTEDPVSSNADEEVHGHGPCESVNRSVMTVTNVAFQQTKSNERHSVSSPDTFQSISMTNVNEKQAIHRPECHSLYIEEKSSPIVSRRLGSEKKSEEFSEQEHSWSIILSQNEASDSSPEDIFSREETVDTGKGLEDTFQLGYSCDKQGGYQIDTGGQDYTELEESFEMCKVEERDIVAHVSHDLLNLQSISVHIQLPPQDGELAKYLGYKEKTENGSILDDEGMDIPLDAAEIRPEPPNSLDLNGSSTRKIKLTAPNINLSLDHSEGSILSDDNLDTPDELDINVDDLDTPDEADSFDYTGQDDQPALGEAVQEDFESIQEYTAEEERADNRLWRTVVIGEQEQRIDMKVIEPYKKVISHGGYYGEGVNAIIVFAACFLPDSSRPDYNYVMENLFLYVISTLELMVAEDYMVVYLNGATPRRKMPGLGWMKKCYQMIDRRAGCLPNEPEMNTLEEEFENKMGDNA

Target ORF information:

RefSeq Version	XM_031901874.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis prune homolog 2 (prune2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031901874.1

ORF Insert Sequence:

ATGAGCTGCA GAAACATTAT TGGGGAGCTG AAACTATTTC TTGACAAATA TGGGATTGAT 
ATACTGATTC TTTTGGCAAG TTATACAACA GAGGAACAGA TGGCAGTGCA ACAAATAGCT 
GTGCACTCAG AGAACCCGGA ACTGTGCAAT CAAGTCTGTT GTGAATTAGA AGAATGTCAG 
AATCCATTCC TTGATCTAGA GCCTTCAGAT TATGGATGTG AGCAATTTCT AGTATATCAC 
CAAGAAAATT CTTCAGTCAC TTGTGAGCAA GTTGCTGCAG TTATTAAAGA AGCTATTAAC 
AGGAGACGAC TAGGAATGAT GCCAAATAGT AGGACTTCAT CAACTGAAGC TGTTGCTGGT 
AGTGCCCCCC TTTCACAAGG ATCTTCTGGC ATCATGGAAT TGTATGGTTC TGACTTAGAC 
CCTCAGCCTA ACCCTGTAAA CTTTGTGGAC AATGTTCAAG ATGGAAATGC TCAAGATATA 
AATGCACAAG CCCAGGTTGA TGTTAACGTG GATCTTGTTA GTCCAGATAG TGGTTTAGCT 
ACTATAAGAA GCAGCCGGTC TTCAAAGGAA AGTTCAGTTT TTCTAAGTGA TGATAGTCCT 
GTAGCAGATG CTGCTGGATC TCATCACAAT TTTCTTCCAG GGATTGATAA CTATAGTCGT 
GTTCCAGAAG GAGTGATAGT AGAGGAGGAA ACACCACCAT CCAGGACCAA CAGTGATAAT 
GCTGAACTCT TTAACTTTGA TTTGTTACCA AATACTCGTT CTGAATCTTC TTCTCATTCT 
GCTGACTATT CTATGGCGGA TGACTTTTTT TTCCAGAGTG ACTCATCTGA AGGACAACAA 
GTTCAAAAGC AACAGCTGTA CAGGAATGAT ATAGCTCATT GTTCAACTAA TTTACTCAAT 
ACAAAAGTGC CAAATATTAG TTTGGTTGAT TTTGATGATG CATTTATGCA TAGTCCTGAA 
AACCATGAGG ACCTATGTGA AAAAACTCCA AGTGCAAGTG ACATTACTGA ATTTGAATCA 
AGCTTATCAT CTGAAATCTT AGGCAGTGCT GATGTTAAAG TCCCACCAAC ACCAATGAAT 
AGTTTAGTTG AAAGTTCTCC CTTAGATAAT GGTCCTCCAA CATTTTTTCC CGAAGACGTG 
GTGCAGAAAA TTAATGAAAT CGGATTCAGT GTATCTGAAA CTCAGGCATA CTGGTGGAAT 
GAAAGTGAGA ATATTGATGC AAATACAGCT CCCATGAATG CTGATGCATG GAGTTCTAGT 
AATCAATCAG TGCTTCACAG ACCAGAGTCC TGGAAAGAGC AAATATCAGG GCAAGACAAT 
GCTAACGCCA GACATGCACC TTCATTTCAA GTACCATGCC CTGGTTTACA TAATGACAAT 
TCAGGCAATC ATGATAAAAA CACAAGACAA GAGAATATAT TAATTCCAGA TTTATGGAAC 
TTCAACAACC CACTGCAGGG CAAATCAGAT CCATTCTCTG GCACTTCAGA TAAAGATGAA 
TTTGGAGACT ATGTGAAAAA ATCTGAAGAC AAATTATGTG CATTCAAAGA AGACAGCAAA 
ACAAAATATT TTGCTAAAAG AAAGGCTAAA AGCAATATAG ATACTGATAT TTCTTTTGAA 
AATACTGCTG AACTACATGA AGAAAGCAGA TATAGTCAAG AGTTTCTAGA TGAAAGTCAA 
TTTATGCTAC AGCAAAGAAG TACTGAGGAT CTAGGGGTGG ATGCAAAAAC CCTACTCCCT 
GGACACATAG AACGAAATCT AGGCATGTGG GATTTTCTTG ATGCAGGACA AGACACAAAC 
ATTGTTGACC ATCATATAAA TTGGGAGGAT CCCTTCTTGT CTTGTGGTCC TTACAGGTGT 
TTGGATTTTA CTGCATCTTA TAAAGGCAAA GACTGCATTG TTTCCCCACC AGATACCAAT 
TACTCAACAT CAGAATCAAA CTTATCTCCA GCATCTGAGG ATGATATGAA GGAGCCTGAA 
AAATCAGATG ATCAAGAAAA ACATCTTCTA AATGAAGGAC ACCAAAGCTC AAATGACCTA 
AATATTCTGA ATAATAGAGA ACTGGCCTCA AATAACAGGC ATGCAGTTTC AGAAAACAAA 
AATGTTAGAA ATCATAGTGA TGTGTCTATA CAGATGATGC CTAAAAGGTC ATGCTTTGGA 
AATTATCTAC ACAGTGATTA TCTAGATAAT GCAATCCATA GTTCTACACC CTCCAAATCA 
AAGAACAATG AACCAAATTA TGAAATGAAC AACCATAAGC ATGATTTGTC TATTCTAAGC 
CCAAATCGAA GCACACTGTT AGAAGCAAGT AGTCACAACT TACTTAGCAA AGAAGAAGCT 
GCATGCTTCA ATTCTGAAAG AGCTCACAAA GTTCCAGACA GTTTTTTGCC TCTTCCAATA 
CATAATAACA CTGTGTTTAA CATCTTGCCA CAGCAAGGCC AAAGTGAAAT TAATGAATGT 
ACTAGGAACT CTTATCCTTC AAATGAAGTC CCCAATGAGG CTCATGGGAA AGGTCAGGGG 
CAATCTACAT TAGAAATAAT GAATAAACAT GATAACAAAG TCAAATCAGG ACAAGATCAC 
CATTTACCAA TCTCAAAGCC ACAGCATAAC CCTTTTTATG AATCACTTTC TCCTGATATA 
AAAGACAAGG TTCTAAACCC ACTTGAAAAC TCTGGTAAAT GTCAGGATAT TTGTGTATTA 
CAAGAAAAGG GAGGAGAGCT TGGAAGTGCT GGTTATACAA ATCAAGATAA CTGGGAAAAT 
GATTTACAAG AGGATACAGA ATCTTCTTCT TTAGATTCAC CTGATGATTT AGAAAGCTCT 
GATTCTAGCT ATTTAAGAAG CTGGAATCTG TTAGTAAAAT CTCCTGTGCT AAAGGATGAT 
CTTCGAATAC CTGGTCAGTT ATCATCTGTA CACGAGCAAG ACGATTATGA AATCAAATCT 
CCTGGTGTAA AGAGCAAGAG TTATAATGAA AAGTCAATTT TATTAAGTCC AGAGGGAAAG 
GTAAATGATA TGAATGATGC CCAGATCTCT TCTGAGAATG ATCATTTGAC TTTAAATAAA 
GTCAAATCAA AAGAAAATGT TTTCCTTAAC GATATTGCTA TTACAAAGAA AACAGATATA 
AGCCACAGTG AACAGGAAGG GTCTGTAATA CAAGGTGAAG GCAGTACAGA CAAGTGGAAT 
AAAACAAAAC TTTGCAGTTA TGAATCAGCA GGAATAAATT CAGAATTATT CCCCCATATG 
TCAAATGTAT ATGTGAAAAA AAAAATAAAC ATTTATGCCT ACAATGAAGA AGATGTACAG 
AGTAACTCAT CACTTTCCGG TGAAACAAGT CCTGATTTGG ATTTTAGCTG GAATAGATTA 
GAAGAAAAAG GTTATAATTC CACTTCAAGT GTTTATGGAT CAGGGTCAAA AAACAATCAA 
CCGTTTAGCT TTATTCAAAA TGAAAGTGCT AATAATAAAT GCCTTAATCC CCAAGGAAAG 
GTTCAGCTCA ACACTGTAAA TAAAGTCCCT ATGAATTTGG ATATTTGGAA TACACAGATA 
TGTGAGGATT CTGAGTCTTC ATCAAGCCCT GAAGGAAATG AAACCAGGAA TCATTCAAAC 
TCATCTGACG AGAAAGCCTT GAATTATTAC CAGAATATAC ACACCTCATT GGACAATGCA 
GATTCTAATT CTACTACTCC ATTTACAGAT GAAGAAAGTC CGGAAATAGA AGATATACTG 
GGCAGTTTTG GTTTTATCCA AGATGGGTGT GTTAATAATG TAATGAAAGA AGAGAAAGAA 
CAAAAAGTTA GTCTAACTAA GAGCAACTGT AAAGCTGCAT GTGAAGAAGG CATGTTAAAC 
CAAGACTGTC TTCAGCATGT TCAAGGTTCC CCAGAGGATA GGTCACAGTC ATATGAAAAT 
GCTCATCAAG ATCATGATAA ACATTATAGG GAAATCTTCA TAAAAGAGGA AACTTTCCAT 
TTAGGAGCTG ATACCAAAGA TGCCTTTACT AGAGGAAGCC AGTCAGCTGA AACATTAGAT 
TTGGGGCATA CTCATAATAA AGTACAAAGC CCAGAGGACA CTTATTTTAC TATTTCACCC 
CAACTGAGCC ATTCTTACCA TGAAAATGGG GACGACAAAC GAAAAGAATG CAGTGATGTT 
GTGGGGACAT GTAGTTCTTT AAATGTTTCA CCACTCTCTC TCTGTTACGA AGACAACTAT 
TCTAACACAG AGAACTATGA AGAGGACTCT TCTGTAAAAA GACAATCATT GAACAACCTG 
AAAACAAACC CTAGACAAAC TAAGTCAACC AACTCTGCTC CTTTAATAAG CCCCATGGAC 
AACATAAACG TAAAAGAGTT TAAACCAGTG TCATGCCATG ATAAAAGTTT CAATAAAACA 
GAGCCTTTTG TGCAATTACA GGCAGAAATG AAAAATGTAA ATAATACAAT TAAAGACACA 
ATAAGTGAAC CACGCACACA AGCTGATCTA TCAAGAGAAA AAAATCTGCA AGGAACAGTA 
GGAGCACTTG GTCAAATAAG CAGACATCCT AATATGGCAC CCCATCATTC AGAAGAAGAC 
TCCTTACTAT TTAATGGTCA GGTGGAGCAA GTAGACTTAA GCAATCAGAT TCAGCAGTAT 
GGAGGGTCTG AAATAAGTGA TTCCATGCTC TTTGAGTGTA TTCCAGATAT TTTAGATGAT 
GGACCGCGGG AATTTTCCCC ATTTTATTCA ATTTCTCCTG ACTTGTGGAG TATATCTGAA 
CATGTGTACA TAAGAAAGTC AGAATATGGC AGTCCAGATG TGCTAAACAG CTGTGACCCC 
AATTCACATG TATCAAAAAC TCCTGATTTG TACAAGCAAT GTGGCCGACC ATATAAAGAT 
GTGTTTGTAT GGCCTGATAC TGCACATAGC AGTCTGTCCC CCAGGGGTTC TGCCGAGCTG 
AGCCAAATAT CAACTGAGGG AAAACACAAT GAAACATACA TGCAAAATGC ACATTTACAC 
AAAAATAATT CTATTTTGCA GCCTTGTATG AAGTGCACAG AGTCAGTAAG CTCCTGCAGC 
AACATGGAAC ATGTTTGTGA GAAACATTCT GTTGATCATT ATAACATAGG GTGCAGCCAA 
GTTAATGCAC CATCATCAGC AATTTCAGTA AAAGGCATTA ATGAACATGT TCTGTTAGAC 
AGGTCAGCAT CAGCTAACCT GATTTTGCAA GAGTGTATGC CTTACACACA AGAAAATGAG 
GAAAATATAC AAAGAGATGT GTTCCAGACA ACAAAAACAC ACGCAGAGCC ATTTATCAAT 
GATAATTCTG CAGCAGAAGT GTTAAACCCT TGCTTACATC AGGGCAAAGT ACCTGGAAAT 
GCAAATGAAG AAAACTGGAA TAATGTCACT TCACCAGCAG ATGGAGATGT TGGACCTCAT 
CATGAAAATG GGGAAGAGAG CGACCGGGTT TGGAATGCTG ACAATATGAC AAGAAGAAGT 
TTAGTTCTTT TTGATAAGGG GCGAGATAGT ATCAAGCTTC AGACTGAGGA TCCGGTTAGT 
TCTAATGCTG ATGAAGAGGT TCACGGTCAT GGTCCCTGTG AAAGTGTAAA CAGATCTGTA 
ATGACAGTCA CTAATGTTGC ATTTCAGCAA ACAAAGTCTA ATGAAAGGCA TTCAGTGAGC 
TCTCCAGATA CATTTCAGTC AATCTCCATG ACAAATGTGA ATGAAAAACA AGCTATTCAC 
CGACCCGAGT GTCATTCTCT GTACATAGAA GAGAAAAGTT CTCCTATTGT GTCTCGAAGG 
TTGGGAAGTG AAAAGAAATC TGAAGAATTT TCTGAACAAG AGCATAGCTG GAGCATCATA 
TTAAGCCAGA ATGAAGCCTC AGACAGTTCA CCTGAAGATA TATTTAGCAG GGAGGAAACA 
GTAGATACTG GTAAAGGTTT GGAAGATACC TTTCAACTAG GATATTCGTG TGATAAACAA 
GGTGGATATC AGATAGACAC TGGAGGTCAG GACTATACAG AGCTCGAGGA AAGCTTTGAA 
ATGTGCAAGG TTGAAGAAAG GGATATCGTT GCACATGTTT CACATGATCT TTTGAATCTC 
CAGAGCATCA GTGTGCACAT TCAGCTGCCT CCTCAAGATG GCGAGCTTGC TAAATATTTA 
GGATATAAAG AAAAGACAGA GAATGGGTCG ATTCTAGATG ATGAGGGAAT GGACATTCCT 
CTTGATGCAG CAGAAATACG ACCTGAACCT CCAAATTCCT TGGATTTAAA TGGCTCCAGC 
ACCAGAAAAA TAAAGCTTAC TGCTCCCAAT ATCAACCTAT CTCTGGACCA CAGTGAAGGA 
TCAATCCTAT CTGATGACAA TCTTGATACC CCAGATGAGC TTGACATTAA TGTTGATGAT 
CTTGACACTC CAGATGAAGC AGACTCCTTT GACTACACAG GCCAGGATGA CCAACCTGCA 
CTTGGGGAAG CCGTACAGGA GGATTTTGAA TCTATACAAG AGTACACAGC AGAAGAGGAA 
AGAGCTGATA ACAGACTATG GAGGACAGTT GTCATTGGAG AGCAAGAGCA GAGGATTGAT 
ATGAAGGTCA TTGAACCATA CAAAAAAGTT ATATCTCATG GAGGTTACTA TGGTGAAGGT 
GTAAATGCCA TCATTGTGTT CGCTGCATGC TTTTTGCCTG ATAGCAGCCG TCCAGACTAC 
AATTATGTCA TGGAAAACCT TTTCCTCTAT GTGATAAGCA CATTGGAGCT TATGGTGGCA 
GAAGACTATA TGGTTGTCTA TTTGAATGGT GCTACACCAA GAAGAAAAAT GCCTGGTTTA 
GGATGGATGA AGAAATGCTA CCAGATGATT GATCGGCGAG CTGGCTGTCT TCCCAATGAA 
CCTGAAATGA ATACCTTGGA GGAGGAATTT GAAAACAAGA TGGGAGACAA CGCCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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prune2 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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