spata20 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following spata20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the spata20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa17237 XM_004918491.3
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa17238 XM_012952854.2
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa17239 XM_002944231.4
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $426.30
$609.00
OXa50479 XM_031894409.1
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa50479 XM_004918491.4
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa50479 XM_031894408.1
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa50480 XM_012952854.3
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa50481 XM_002944231.5
Latest version!
Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa17237
    Clone ID Related Accession (Same CDS sequence) XM_004918491.3
    Accession Version XM_004918491.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004918491.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGTACTGG
    TATCCGTGGG GTCAAGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATG TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAGGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGATATCGA CGGAGCAGCA GAGCCTCCAC CGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG GCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGTAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA CATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACATCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918548.1
    CDS69..2423
    Translation

    Target ORF information:

    RefSeq Version XM_004918491.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918491.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGTACTGG
    TATCCGTGGG GTCAAGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATG TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAGGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGATATCGA CGGAGCAGCA GAGCCTCCAC CGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG GCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGTAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA CATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACATCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17238
    Clone ID Related Accession (Same CDS sequence) XM_012952854.2
    Accession Version XM_012952854.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012952854.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTCCG GCAGTACGCA GACGCCAACA GGCCGTGTTA ATCGTCTTAT CAATGAGAAG 
    TCTCTGTACC TTCAGCAGCA TGCACGGAAC CCTGTGTACT GGTATCCGTG GGGTCAAGAG
    GCTTTTAGCA GGGCAGCCAG AGAGATGAAG CCAATATTTC TCTCGGTTGG CTATTCCACC
    TGCCACTGGT GCCACGTAAT GGAGAGAGAA TCGTTTGAGG ATGAAGAGAT TGGACGGATT
    CTAAATGAGA ATTTCATCTG CGTGAAAGTA GATCGGGAGG AGAGGCCGGA TGTAGACAAG
    GTGTACATGA CATTCCTACA GGCTACTGAT AGTGGTGGCG GGTGGCCCAT GAGTGTTTGG
    CTTACACCTG ATCTCCGACC TTTTGTGGGC GGGACCTACT TCCCACCTGA AGACGGCGTG
    CGCAGGGTCA GCTTTCGGAC TGTTCTGCTA CGGATAGTTG AACAGTGGAA AGAAAACAGG
    GCCTTTCTAT GTGAGAGAAG TGAGCGGATT CTTAGTGTAC TACAGAGCAG CTCTGATATC
    GACGGAGCAG CAGAGCCTCC ACCGAGCCTC CCAGTGCAGA AACTCTGCTT CCAACAGCTG
    GAAAGAATAT TCGATGAGGA GTATGGGGGT TTTGGAGAAT TTCCCAAATT TCCCACCCCA
    GTGAATTTTA GCTTCCTTTT CTGTTTGTGG GCCTTAAGTA AAGGAAGCCC TGAAGGGACT
    CAAGCTCTGC ATATGGCAGT CCATACACTG AAATGGATGA TGTACGGAGG TATCCATGAT
    CATATTGGAA AGGGCTTTCA TCGGTACTCC ACTGACCAGA CCTGGCATGT GCCTCACTTT
    GAGAAAATGC TGTATGATCA AGGCCAGTTA GCTGTAGCAT ATGCAGAAGC CTTTCAGATA
    TCTGGAAAGG AAATCTTTAG TGACGCTGCC CATGATATAC TACAGTACGT GCTTCAAAAT
    CTGAGTGATG ATGCTGGTGG GTTCTACAGC GCAGAGGATG CAGACTCGCT CCCAAATGCC
    CAGTCCAAGG AAAAGAAAGA GGGTGCTTTT GCCACCTGGA CTGCTAAGGA AATCCAACAG
    CTTCTCCCTG ACATGGAGGA AGCAAATGGC AACACCTTTG GGGACATCTT TATGCATCAT
    TATGGCATGA AGGAAGAGGG CAACGTCTCT GCTAGCCAGG ATATTCATGG AGAGTTGCAA
    GGCCAAAACG TACTCATCGT GCGTTCATCC CTGGAGCTCA CCGCTGCCAA GTTTGGTCTG
    GATGTTGCAC GGGTGCAAAC CATTCTTAGT ATGTGTCGGG ATCGATTATA TAAAGCTCGC
    AGGTTGCGCC CTCCTCCGCA GAGAGACACC AACATTTTGG CTTCTTGGAA TGGTTTAATG
    CTTTCAGGCT TGGCTCGCTG CGGAGTGATC CTCAGAGATG AAGAGTACAT CGAGAGAGCC
    AAATTGGCAG CTTCTTTCTT GCATGAAAAT ATGTATGATC TGAAGAGTGG GATATTGTTA
    AGAAGCTTCT ACAAGGGCCA TCAACCAATT GCCGATCTGG TTCCTGGGTT TCTTGATGAC
    TATGCCTTCA TGGTCCGAGG TTTACTGGAC TTGTATGAAG CGTGTTTGGA CCAGTTTTAT
    CTAGAATGGG CTCTACAACT TCAGGACAGG CAAGACCAAC TATTTTGGGA CGCCAAAGGA
    TCGGGTTATT TCTGTAGCGA TGCCAGCGAC TCAAGTATTC TACTGCGTTT GAAGGATGAT
    CAAGATGGAG CAGAGCCCAG CGGAAACTCT GTGTCTGTGG TTAACCTCTT GCGGCTGGCC
    TGTTACATCG GTCGTACAGA ATTTACGGAG AGATCTGGCC AGATACTGGC TGCGTTTTCG
    GAACGGTTGC TGAAGGTCCC GGCCTCTCTT CCTGAGATGG TTCGGGGAAA CATGATCTAT
    CACCAAACAG TCAAACAGGT GGTAGTGTGT GGCGACAAAG AAGATCCAAA CACTAGAGAA
    CTGCTGGAAG CTGCACAGAG CATGTATGTT CCTAACAAGG TGCTGATCCT CGCAGACAGG
    ACGCAGACAA GTTTCCTTTA CAGCCATCTG CCCTTTCTCC TGTCTCTGGA GCAGAAGGAC
    GGCAAAGCCA CAGCCTACGT GTGTGAGAAT TTCACCTGCT CTCTGCCAAC CACCTCTCCG
    GCTGACCTGC GCACCATGCT AGCACCCTCT GGCACCCAGC GATCACGGCC TCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808308.1
    CDS189..2405
    Translation

    Target ORF information:

    RefSeq Version XM_012952854.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952854.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTCCG GCAGTACGCA GACGCCAACA GGCCGTGTTA ATCGTCTTAT CAATGAGAAG 
    TCTCTGTACC TTCAGCAGCA TGCACGGAAC CCTGTGTACT GGTATCCGTG GGGTCAAGAG
    GCTTTTAGCA GGGCAGCCAG AGAGATGAAG CCAATATTTC TCTCGGTTGG CTATTCCACC
    TGCCACTGGT GCCACGTAAT GGAGAGAGAA TCGTTTGAGG ATGAAGAGAT TGGACGGATT
    CTAAATGAGA ATTTCATCTG CGTGAAAGTA GATCGGGAGG AGAGGCCGGA TGTAGACAAG
    GTGTACATGA CATTCCTACA GGCTACTGAT AGTGGTGGCG GGTGGCCCAT GAGTGTTTGG
    CTTACACCTG ATCTCCGACC TTTTGTGGGC GGGACCTACT TCCCACCTGA AGACGGCGTG
    CGCAGGGTCA GCTTTCGGAC TGTTCTGCTA CGGATAGTTG AACAGTGGAA AGAAAACAGG
    GCCTTTCTAT GTGAGAGAAG TGAGCGGATT CTTAGTGTAC TACAGAGCAG CTCTGATATC
    GACGGAGCAG CAGAGCCTCC ACCGAGCCTC CCAGTGCAGA AACTCTGCTT CCAACAGCTG
    GAAAGAATAT TCGATGAGGA GTATGGGGGT TTTGGAGAAT TTCCCAAATT TCCCACCCCA
    GTGAATTTTA GCTTCCTTTT CTGTTTGTGG GCCTTAAGTA AAGGAAGCCC TGAAGGGACT
    CAAGCTCTGC ATATGGCAGT CCATACACTG AAATGGATGA TGTACGGAGG TATCCATGAT
    CATATTGGAA AGGGCTTTCA TCGGTACTCC ACTGACCAGA CCTGGCATGT GCCTCACTTT
    GAGAAAATGC TGTATGATCA AGGCCAGTTA GCTGTAGCAT ATGCAGAAGC CTTTCAGATA
    TCTGGAAAGG AAATCTTTAG TGACGCTGCC CATGATATAC TACAGTACGT GCTTCAAAAT
    CTGAGTGATG ATGCTGGTGG GTTCTACAGC GCAGAGGATG CAGACTCGCT CCCAAATGCC
    CAGTCCAAGG AAAAGAAAGA GGGTGCTTTT GCCACCTGGA CTGCTAAGGA AATCCAACAG
    CTTCTCCCTG ACATGGAGGA AGCAAATGGC AACACCTTTG GGGACATCTT TATGCATCAT
    TATGGCATGA AGGAAGAGGG CAACGTCTCT GCTAGCCAGG ATATTCATGG AGAGTTGCAA
    GGCCAAAACG TACTCATCGT GCGTTCATCC CTGGAGCTCA CCGCTGCCAA GTTTGGTCTG
    GATGTTGCAC GGGTGCAAAC CATTCTTAGT ATGTGTCGGG ATCGATTATA TAAAGCTCGC
    AGGTTGCGCC CTCCTCCGCA GAGAGACACC AACATTTTGG CTTCTTGGAA TGGTTTAATG
    CTTTCAGGCT TGGCTCGCTG CGGAGTGATC CTCAGAGATG AAGAGTACAT CGAGAGAGCC
    AAATTGGCAG CTTCTTTCTT GCATGAAAAT ATGTATGATC TGAAGAGTGG GATATTGTTA
    AGAAGCTTCT ACAAGGGCCA TCAACCAATT GCCGATCTGG TTCCTGGGTT TCTTGATGAC
    TATGCCTTCA TGGTCCGAGG TTTACTGGAC TTGTATGAAG CGTGTTTGGA CCAGTTTTAT
    CTAGAATGGG CTCTACAACT TCAGGACAGG CAAGACCAAC TATTTTGGGA CGCCAAAGGA
    TCGGGTTATT TCTGTAGCGA TGCCAGCGAC TCAAGTATTC TACTGCGTTT GAAGGATGAT
    CAAGATGGAG CAGAGCCCAG CGGAAACTCT GTGTCTGTGG TTAACCTCTT GCGGCTGGCC
    TGTTACATCG GTCGTACAGA ATTTACGGAG AGATCTGGCC AGATACTGGC TGCGTTTTCG
    GAACGGTTGC TGAAGGTCCC GGCCTCTCTT CCTGAGATGG TTCGGGGAAA CATGATCTAT
    CACCAAACAG TCAAACAGGT GGTAGTGTGT GGCGACAAAG AAGATCCAAA CACTAGAGAA
    CTGCTGGAAG CTGCACAGAG CATGTATGTT CCTAACAAGG TGCTGATCCT CGCAGACAGG
    ACGCAGACAA GTTTCCTTTA CAGCCATCTG CCCTTTCTCC TGTCTCTGGA GCAGAAGGAC
    GGCAAAGCCA CAGCCTACGT GTGTGAGAAT TTCACCTGCT CTCTGCCAAC CACCTCTCCG
    GCTGACCTGC GCACCATGCT AGCACCCTCT GGCACCCAGC GATCACGGCC TCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa17239
    Clone ID Related Accession (Same CDS sequence) XM_002944231.4
    Accession Version XM_002944231.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002944231.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGTACTGG
    TATCCGTGGG GTCAAGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATG TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAGGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGATATCGA CGGAGCAGCA GAGCCTCCAC CGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG GCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGTAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA CATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACATCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGCC
    GCGTCCCAGT CTCTAGTGCT GGCAGCCTCC CAGATCCTGA AAGGGGACGT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002944277.2
    CDS69..2285
    Translation

    Target ORF information:

    RefSeq Version XM_002944231.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002944231.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGTACTGG
    TATCCGTGGG GTCAAGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATG TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAGGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGATATCGA CGGAGCAGCA GAGCCTCCAC CGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG GCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGTAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA CATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACATCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGCC
    GCGTCCCAGT CTCTAGTGCT GGCAGCCTCC CAGATCCTGA AAGGGGACGT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50479
    Clone ID Related Accession (Same CDS sequence) XM_031894408.1 , XM_004918491.4 , XM_031894409.1
    Accession Version XM_031894408.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750268.1
    CDS62..2416
    Translation

    Target ORF information:

    RefSeq Version XM_031894408.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894408.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50479
    Clone ID Related Accession (Same CDS sequence) XM_031894408.1 , XM_004918491.4 , XM_031894409.1
    Accession Version XM_004918491.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004918491.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004918548.2
    CDS62..2416
    Translation

    Target ORF information:

    RefSeq Version XM_004918491.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004918491.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50479
    Clone ID Related Accession (Same CDS sequence) XM_031894408.1 , XM_004918491.4 , XM_031894409.1
    Accession Version XM_031894409.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031750269.1
    CDS62..2416
    Translation

    Target ORF information:

    RefSeq Version XM_031894409.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031894409.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGTG
    CTGATCCTCG CAGACAGGAC GCAGACAAGT TTCCTTTACA GCCATCTGCC CTTTCTCCTG
    TCTCTGGAGC AGAAGGACGG CAAAGCCACA GCCTACGTGT GTGAGAATTT CACCTGCTCT
    CTGCCAACCA CCTCTCCGGC TGACCTGCGC ACCATGCTAG CACCCTCTGG CACCCAGCGA
    TCACGGCCTC TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50480
    Clone ID Related Accession (Same CDS sequence) XM_012952854.3
    Accession Version XM_012952854.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_012952854.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTCCG GCAGTACGCA GACGCCAACA GGCCGTGTTA ATCGTCTTAT CAATGAGAAG 
    TCTCTGTACC TTCAGCAGCA TGCACGGAAC CCTGTGGACT GGTATCCGTG GGGTCAGGAG
    GCTTTTAGCA GGGCAGCCAG AGAGATGAAG CCAATATTTC TCTCGGTTGG CTATTCCACC
    TGCCACTGGT GCCACGTAAT GGAGAGAGAA TCGTTTGAGG ATGAAGAGAT TGGACGGATT
    CTAAATGAGA ATTTCATCTG CGTGAAAGTA GATCGGGAGG AGAGGCCGGA TATAGACAAG
    GTGTACATGA CATTCCTACA GGCTACTGAT AGTGGTGGCG GGTGGCCCAT GAGTGTTTGG
    CTTACACCTG ATCTCCGACC TTTTGTGGGC GGGACCTACT TCCCACCTGA AGACGGCGTG
    CGCAAGGTCA GCTTTCGGAC TGTTCTGCTA CGGATAGTTG AACAGTGGAA AGAAAACAGG
    GCCTTTCTAT GTGAGAGAAG TGAGCGGATT CTTAGTGTAC TACAGAGCAG CTCTGGTATC
    GACGGAGCAG CAGAGCCTCC ACTGAGCCTC CCAGTGCAGA AACTCTGCTT CCAACAGCTG
    GAAAGAATAT TCGATGAGGA GTATGGGGGT TTTGGAGAAT TTCCCAAATT TCCCACCCCA
    GTGAATTTTA GCTTCCTTTT CTGTTTGTGG GCCTTAAGTA AAGGAAGCCC TGAAGGGACT
    CAAGCTCTGC ATATGGCAGT CCATACACTG AAATGGATGA TGTACGGAGG TATCCATGAT
    CATATTGGAA AGGGCTTTCA TCGGTACTCC ACTGACCAGA CCTGGCATGT GCCTCACTTT
    GAGAAAATGC TGTATGATCA AGCCCAGTTA GCTGTAGCAT ATGCAGAAGC CTTTCAGATA
    TCTGGAAAGG AAATCTTTAG TGACGCTGCC CATGATATAC TACAGTACGT GCTTCAAAAT
    CTGAGTGATG ATGCTGGTGG GTTCTACAGC GCAGAGGATG CAGACTCGCT CCCAAATGCC
    CAGTCCAAGG AAAAGAAAGA GGGTGCTTTT GCCACCTGGA CTGCTAAGGA AATCCAACAG
    CTTCTCCCTG ACATGGAGGA AGCAAATGGC AACACCTTTG GGGACATCTT TATGCATCAT
    TATGGCATGA AGGAAGAGGG CAACGTCTCT GCTAGCCAGG ATATTCATGG AGAGTTGCAA
    GGCCAAAACG TACTCATCGT GCGTTCATCC CTGGAGCTCA CCGCTGCCAA GTTTGGTCTG
    GATGTTGCAC GGGTGCAAAC CATTCTTAGC ATGTGTCGGG ATCGATTATA TAAAGCTCGC
    AGGTTGCGCC CTCCTCCGCA GAGAGACACC AAAATTTTGG CTTCTTGGAA TGGTTTAATG
    CTTTCAGGCT TGGCTCGCTG CGGAGTGATC CTCAGAGATG AAGAGTACAT CGAGAGAGCC
    AAATTGGCAG CTTCTTTCTT GCATGAAAAT ATGTATGATC TGAAGAGTGG GATATTGTTA
    AGAAGCTTCT ACAAGGGCCA TCAACCAATT GCCGATCTGG TTCCTGGGTT TCTTGATGAC
    TATGCCTTCA TGGTCCGAGG TTTACTGGAC TTGTATGAAG CGTGTTTGGA CCAGTTTTAT
    CTAGAATGGG CTCTACAACT TCAGGACAGG CAAGACCAAC TATTTTGGGA CGCCAAAGGA
    TCGGGTTATT TCTGTAGCGA TGCCAGCGAC TCAAGTATTC TACTGCGTTT GAAGGATGAT
    CAAGATGGAG CAGAGCCCAG CGGAAACTCT GTGTCTGTGG TTAACCTCTT GCGGCTGGCC
    TGTTACACCG GTCGTACAGA ATTTACGGAG AGATCTGGCC AGATACTGGC TGCGTTTTCG
    GAACGGTTGC TGAAGGTCCC GGCCTCTCTT CCTGAGATGG TTCGGGGAAA CATGATCTAT
    CACCAAACAG TCAAACAGGT GGTAGTGTGT GGCGACAAAG AAGATCCAAA CACTAGAGAA
    CTGCTGGAAG CTGCACAGAG CATGTATGTT CCTAACAAGG TGCTGATCCT CGCAGACAGG
    ACGCAGACAA GTTTCCTTTA CAGCCATCTG CCCTTTCTCC TGTCTCTGGA GCAGAAGGAC
    GGCAAAGCCA CAGCCTACGT GTGTGAGAAT TTCACCTGCT CTCTGCCAAC CACCTCTCCG
    GCTGACCTGC GCACCATGCT AGCACCCTCT GGCACCCAGC GATCACGGCC TCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012808308.2
    CDS178..2394
    Translation

    Target ORF information:

    RefSeq Version XM_012952854.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012952854.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGGCTTCCG GCAGTACGCA GACGCCAACA GGCCGTGTTA ATCGTCTTAT CAATGAGAAG 
    TCTCTGTACC TTCAGCAGCA TGCACGGAAC CCTGTGGACT GGTATCCGTG GGGTCAGGAG
    GCTTTTAGCA GGGCAGCCAG AGAGATGAAG CCAATATTTC TCTCGGTTGG CTATTCCACC
    TGCCACTGGT GCCACGTAAT GGAGAGAGAA TCGTTTGAGG ATGAAGAGAT TGGACGGATT
    CTAAATGAGA ATTTCATCTG CGTGAAAGTA GATCGGGAGG AGAGGCCGGA TATAGACAAG
    GTGTACATGA CATTCCTACA GGCTACTGAT AGTGGTGGCG GGTGGCCCAT GAGTGTTTGG
    CTTACACCTG ATCTCCGACC TTTTGTGGGC GGGACCTACT TCCCACCTGA AGACGGCGTG
    CGCAAGGTCA GCTTTCGGAC TGTTCTGCTA CGGATAGTTG AACAGTGGAA AGAAAACAGG
    GCCTTTCTAT GTGAGAGAAG TGAGCGGATT CTTAGTGTAC TACAGAGCAG CTCTGGTATC
    GACGGAGCAG CAGAGCCTCC ACTGAGCCTC CCAGTGCAGA AACTCTGCTT CCAACAGCTG
    GAAAGAATAT TCGATGAGGA GTATGGGGGT TTTGGAGAAT TTCCCAAATT TCCCACCCCA
    GTGAATTTTA GCTTCCTTTT CTGTTTGTGG GCCTTAAGTA AAGGAAGCCC TGAAGGGACT
    CAAGCTCTGC ATATGGCAGT CCATACACTG AAATGGATGA TGTACGGAGG TATCCATGAT
    CATATTGGAA AGGGCTTTCA TCGGTACTCC ACTGACCAGA CCTGGCATGT GCCTCACTTT
    GAGAAAATGC TGTATGATCA AGCCCAGTTA GCTGTAGCAT ATGCAGAAGC CTTTCAGATA
    TCTGGAAAGG AAATCTTTAG TGACGCTGCC CATGATATAC TACAGTACGT GCTTCAAAAT
    CTGAGTGATG ATGCTGGTGG GTTCTACAGC GCAGAGGATG CAGACTCGCT CCCAAATGCC
    CAGTCCAAGG AAAAGAAAGA GGGTGCTTTT GCCACCTGGA CTGCTAAGGA AATCCAACAG
    CTTCTCCCTG ACATGGAGGA AGCAAATGGC AACACCTTTG GGGACATCTT TATGCATCAT
    TATGGCATGA AGGAAGAGGG CAACGTCTCT GCTAGCCAGG ATATTCATGG AGAGTTGCAA
    GGCCAAAACG TACTCATCGT GCGTTCATCC CTGGAGCTCA CCGCTGCCAA GTTTGGTCTG
    GATGTTGCAC GGGTGCAAAC CATTCTTAGC ATGTGTCGGG ATCGATTATA TAAAGCTCGC
    AGGTTGCGCC CTCCTCCGCA GAGAGACACC AAAATTTTGG CTTCTTGGAA TGGTTTAATG
    CTTTCAGGCT TGGCTCGCTG CGGAGTGATC CTCAGAGATG AAGAGTACAT CGAGAGAGCC
    AAATTGGCAG CTTCTTTCTT GCATGAAAAT ATGTATGATC TGAAGAGTGG GATATTGTTA
    AGAAGCTTCT ACAAGGGCCA TCAACCAATT GCCGATCTGG TTCCTGGGTT TCTTGATGAC
    TATGCCTTCA TGGTCCGAGG TTTACTGGAC TTGTATGAAG CGTGTTTGGA CCAGTTTTAT
    CTAGAATGGG CTCTACAACT TCAGGACAGG CAAGACCAAC TATTTTGGGA CGCCAAAGGA
    TCGGGTTATT TCTGTAGCGA TGCCAGCGAC TCAAGTATTC TACTGCGTTT GAAGGATGAT
    CAAGATGGAG CAGAGCCCAG CGGAAACTCT GTGTCTGTGG TTAACCTCTT GCGGCTGGCC
    TGTTACACCG GTCGTACAGA ATTTACGGAG AGATCTGGCC AGATACTGGC TGCGTTTTCG
    GAACGGTTGC TGAAGGTCCC GGCCTCTCTT CCTGAGATGG TTCGGGGAAA CATGATCTAT
    CACCAAACAG TCAAACAGGT GGTAGTGTGT GGCGACAAAG AAGATCCAAA CACTAGAGAA
    CTGCTGGAAG CTGCACAGAG CATGTATGTT CCTAACAAGG TGCTGATCCT CGCAGACAGG
    ACGCAGACAA GTTTCCTTTA CAGCCATCTG CCCTTTCTCC TGTCTCTGGA GCAGAAGGAC
    GGCAAAGCCA CAGCCTACGT GTGTGAGAAT TTCACCTGCT CTCTGCCAAC CACCTCTCCG
    GCTGACCTGC GCACCATGCT AGCACCCTCT GGCACCCAGC GATCACGGCC TCTGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa50481
    Clone ID Related Accession (Same CDS sequence) XM_002944231.5
    Accession Version XM_002944231.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2217bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product spermatogenesis-associated protein 20 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030686.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002944231.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGCC
    GCGTCCCAGT CTCTAGTGCT GGCAGCCTCC CAGATCCTGA AAGGGGACGT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002944277.3
    CDS62..2278
    Translation

    Target ORF information:

    RefSeq Version XM_002944231.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis spermatogenesis associated 20 (spata20), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002944231.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    ATGCTTCGCT GTGTGTCCGG CCTGTGCAGG GCAAGGGGGG TTCTGCCCCG GGCTACTCTG 
    GGCTTCTGTC CGTCTCCGAG GAACCCCGGG CACCGCCGTG GGAAGTGGAG AGGAGGTGAG
    ATATTTGAAG TATGCAAGAT GGCTTCCGGC AGTACGCAGA CGCCAACAGG CCGTGTTAAT
    CGTCTTATCA ATGAGAAGTC TCTGTACCTT CAGCAGCATG CACGGAACCC TGTGGACTGG
    TATCCGTGGG GTCAGGAGGC TTTTAGCAGG GCAGCCAGAG AGATGAAGCC AATATTTCTC
    TCGGTTGGCT ATTCCACCTG CCACTGGTGC CACGTAATGG AGAGAGAATC GTTTGAGGAT
    GAAGAGATTG GACGGATTCT AAATGAGAAT TTCATCTGCG TGAAAGTAGA TCGGGAGGAG
    AGGCCGGATA TAGACAAGGT GTACATGACA TTCCTACAGG CTACTGATAG TGGTGGCGGG
    TGGCCCATGA GTGTTTGGCT TACACCTGAT CTCCGACCTT TTGTGGGCGG GACCTACTTC
    CCACCTGAAG ACGGCGTGCG CAAGGTCAGC TTTCGGACTG TTCTGCTACG GATAGTTGAA
    CAGTGGAAAG AAAACAGGGC CTTTCTATGT GAGAGAAGTG AGCGGATTCT TAGTGTACTA
    CAGAGCAGCT CTGGTATCGA CGGAGCAGCA GAGCCTCCAC TGAGCCTCCC AGTGCAGAAA
    CTCTGCTTCC AACAGCTGGA AAGAATATTC GATGAGGAGT ATGGGGGTTT TGGAGAATTT
    CCCAAATTTC CCACCCCAGT GAATTTTAGC TTCCTTTTCT GTTTGTGGGC CTTAAGTAAA
    GGAAGCCCTG AAGGGACTCA AGCTCTGCAT ATGGCAGTCC ATACACTGAA ATGGATGATG
    TACGGAGGTA TCCATGATCA TATTGGAAAG GGCTTTCATC GGTACTCCAC TGACCAGACC
    TGGCATGTGC CTCACTTTGA GAAAATGCTG TATGATCAAG CCCAGTTAGC TGTAGCATAT
    GCAGAAGCCT TTCAGATATC TGGAAAGGAA ATCTTTAGTG ACGCTGCCCA TGATATACTA
    CAGTACGTGC TTCAAAATCT GAGTGATGAT GCTGGTGGGT TCTACAGCGC AGAGGATGCA
    GACTCGCTCC CAAATGCCCA GTCCAAGGAA AAGAAAGAGG GTGCTTTTGC CACCTGGACT
    GCTAAGGAAA TCCAACAGCT TCTCCCTGAC ATGGAGGAAG CAAATGGCAA CACCTTTGGG
    GACATCTTTA TGCATCATTA TGGCATGAAG GAAGAGGGCA ACGTCTCTGC TAGCCAGGAT
    ATTCATGGAG AGTTGCAAGG CCAAAACGTA CTCATCGTGC GTTCATCCCT GGAGCTCACC
    GCTGCCAAGT TTGGTCTGGA TGTTGCACGG GTGCAAACCA TTCTTAGCAT GTGTCGGGAT
    CGATTATATA AAGCTCGCAG GTTGCGCCCT CCTCCGCAGA GAGACACCAA AATTTTGGCT
    TCTTGGAATG GTTTAATGCT TTCAGGCTTG GCTCGCTGCG GAGTGATCCT CAGAGATGAA
    GAGTACATCG AGAGAGCCAA ATTGGCAGCT TCTTTCTTGC ATGAAAATAT GTATGATCTG
    AAGAGTGGGA TATTGTTAAG AAGCTTCTAC AAGGGCCATC AACCAATTGC CGATCTGGTT
    CCTGGGTTTC TTGATGACTA TGCCTTCATG GTCCGAGGTT TACTGGACTT GTATGAAGCG
    TGTTTGGACC AGTTTTATCT AGAATGGGCT CTACAACTTC AGGACAGGCA AGACCAACTA
    TTTTGGGACG CCAAAGGATC GGGTTATTTC TGTAGCGATG CCAGCGACTC AAGTATTCTA
    CTGCGTTTGA AGGATGATCA AGATGGAGCA GAGCCCAGCG GAAACTCTGT GTCTGTGGTT
    AACCTCTTGC GGCTGGCCTG TTACACCGGT CGTACAGAAT TTACGGAGAG ATCTGGCCAG
    ATACTGGCTG CGTTTTCGGA ACGGTTGCTG AAGGTCCCGG CCTCTCTTCC TGAGATGGTT
    CGGGGAAACA TGATCTATCA CCAAACAGTC AAACAGGTGG TAGTGTGTGG CGACAAAGAA
    GATCCAAACA CTAGAGAACT GCTGGAAGCT GCACAGAGCA TGTATGTTCC TAACAAGGCC
    GCGTCCCAGT CTCTAGTGCT GGCAGCCTCC CAGATCCTGA AAGGGGACGT AGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.