SPATA20 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	SPATA20
Entrez Gene ID	511363
Full Name	spermatogenesis associated 20
General protein information	Preferred Names spermatogenesis associated 20 Names spermatogenesis-associated protein 20
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024979934.1	XP_024835702.1	spermatogenesis-associated protein 20 isoform X1
NM_001083407.1	NP_001076876.1	spermatogenesis-associated protein 20

Pathways from BioSystems

Homologies

	SPATA20	XP_001099622.1
Danio rerio (zebrafish)	spata20	XP_001920588.3
Drosophila melanogaster (fruit fly)	CG8613	NP_610953.1
Homo sapiens (human)	SPATA20	NP_001245301.1
Pan troglodytes (chimpanzee)	SPATA20	XP_001170552.1
Bos taurus (cattle)	SPATA20	NP_001076876.1
Arabidopsis thaliana (thale cress)	AT4G03200	NP_192229.3
Canis lupus familiaris (dog)	SPATA20	XP_005624701.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008252	XP_317217.4
Mus musculus (house mouse)	Spata20	NP_659076.2
Rattus norvegicus (Norway rat)	Spata20	NP_955434.1
Gallus gallus (chicken)	SPATA20	XP_420103.3
Caenorhabditis elegans (roundworm)	B0495.5	NP_495615.2
Xenopus tropicalis (tropical clawed frog)	spata20	XP_004918550.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following SPATA20 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPATA20 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OBa36091	NM_001083407.1 Latest version!	Bos taurus spermatogenesis associated 20 (SPATA20), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
OBa29891	XM_024979934.1 Latest version!	Bos taurus spermatogenesis associated 20 (SPATA20), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SPATA20 ( NM_001083407.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001076876.1 Bos taurus spermatogenesis associated 20 (SPATA20), mRNA.

Price & Availability↑

CloneID	OBa36091
Clone ID Related Accession (Same CDS sequence)	NM_001083407.1
Accession Version	NM_001083407.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	spermatogenesis-associated protein 20
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC123689.1. On Mar 27, 2007 this sequence version replaced XM_880891.2. ##Evidence-Data-START## Transcript exon combination :: BC123689.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN03145412, SAMN03145413 [ECO:0000350] ##Evidence-Data-END##

ATGCTGGGCG CGCGGGCCTT ACGGTGCCGC GGCTTCCTGC TGCCCGGGGC CGGGCCAGTC 
CTCGCCTTGA GCTACAGGGG TAGCTCCGCC CGGGACAAGG ACCGAAGTGT GACGGTCAGT 
AGTTCAGTGC CCATGCCTGC TGGAGGGAAG GGGAGCCGGA CTAACTGCTC TCAGTCCACG 
CCCCAGAAGG TCCCCAACCG CCTGATCAAT GAGAAGTCAC CGTACCTCCT GCAACATGCC 
TACAACCCTG TGGACTGGTA CCCCTGGGGA CAGGAAGCCT TCGACAAGGC CAAGAAAGAA 
AACAAGCCAA TTTTTCTTTC AGTGGGGTAC TCCACCTGCC ACTGGTGCCA CATGATGGAG 
GAGGAGTCCT TCCAGAATGA GGAGATTGGC CGCCTGCTCA GCGAGGACTT TGTCAGTGTG 
AAGGTGGACC GGGAGGAGCG GCCTGACGTG GACAAGGTGT ATATGACTTT TGTGCAGGCC 
ACCAGCAGTG GTGGGGGCTG GCCCATGAGT GTGTGGCTGA CTCCCGACCT ACAGCCCTTT 
GTGGGCGGCA CCTATTTTCC CCCTGAGGAT GGCTTGACCC GAGTTGGCTT CCGCACAGTG 
TTGATGAGGA TACGGGACCA GTGGAAACAG AACAAGAGTA CCCTGCTAGA AAACAGTCAG 
CGGGTCACCA CGGCTCTGCT GGCTCGGTCG GCGATCAGTA TGGGTGACCG CCAGTTGCCA 
CCTTCCGCTG CCACCATGAA CAGCCGCTGC TTCCAGCAGC TGGATGAGGG CTATGATGAG 
GAGTATGGTG GCTTCGCTGA GGCCCCCAAG TTCCCCACAC CAGTGATCCT GAGTTTCCTG 
TTCTCCTACT GGCTCAGCCA CCGGCTAACC CAGGACGGCT CTCGGGCCCA GCAGATGGCC 
TTGCATACCC TGAAGATGAT GGCCAACGGG GGTATCCGAG ACCACGTGGG GCAGGGCTTC 
CACCGCTACT CCACGGACCG CCAGTGGCAC GTTCCCCACT TTGAGAAGAT GCTGTATGAC 
CAGGCACAGC TCACGGTGGC CTATTCACAA GCTTTCCAGA TCTCTGGTGA CGAGTTCTAC 
TCCGAAGTTG CCAAAGGTAT CCTGCAGTAC GTCGTTCGGA ACCTGAGTCA CCGGTCTGGA 
GGCTTCTATA GTGCAGAGGA CGCGGACTCC CCGCCGGAGC GGGGCATGCG GCCCAAAGAG 
GGCGCCTTCT ACGTGTGGAC TGTCAAGGAG GTCCAGCATC TCCTCCCTGA GCCTGTGCTG 
GGCGCCACCG AGCCTCTGAC CTCAGGCCAG CTCCTCATGA AGCACTATGG GCTCACGGAG 
GCTGGCAACA TCAGCCCCAG TCAGGACCCC AAGGGGGAGC TGCAGGGCCA GAACGTGCTG 
ACCGTCCGAT ATTCGCTGGA GCTGACTGCC GCCCGCTTTG GCCTGGACGT GGAGGCTGTA 
CGAACCTTAC TCAATTCAGG CCTCGAGAAG CTCTTCCAGG CCCGGAAACA TCGGCCGAAG 
CCGCACTTGG ACAGCAAGAT GCTGGCTGCC TGGAATGGAC TGATGGTGTC TGGCTTTGCT 
GTGACTGGGG CTGTTCTGGG CCAGGAGAGG GTGATCAACT ACGCCATCAA TGGTGCCAAG 
TTCCTGAAGC GGCACATGTT TGATGTGGCC AGTGGCCGCC TGATGCGGAC CTGCTATGCA 
GGCTCTGGTG GGACTGTGGA ACACAGCAAC CCGCCCTGCT GGGGCTTCCT GGAGGACTAT 
GCCTTCGTGG TGAGGGGCCT GCTGGACTTG TACGAGGCCT CACAGGAGAG TGCGTGGCTC 
GAGTGGGCTC TGCGGCTGCA GGACACACAG GACAGGCTCT TCTGGGACTC CCGGGGCGGC 
GGCTACTTCT GCAGTGAGGC CGAGCTGGGG GCTGGCCTGC CGCTGCGTCT GAAGGACGAC 
CAGGATGGTG CAGAGCCCAG TGCCAACTCC GTGTCGGCCC ACAACCTGCT TCGGCTGCAC 
GGTTTCACGG GCCACAAGGA CTGGATGGAC AAGTGTGTGT GCCTGTTGAC CGCCTTCTCT 
GAGCGCATGC GCCGTGTGCC CGTGGCATTG CCTGAGATGG TCCGCGCCCT CTCAGCCCAC 
CAGCAGACCC TCAAGCAGAT TGTGATCTGT GGGGACCCCC AGGCCAAGGA TACCAAGGCT 
CTGTTGCAGT GTGTCCACTC CATCTACATC CCTAACAAGG TGCTGATTCT GGCTGATGGG 
GACCCCTCGA GCTTCCTGTC CCGCCAGCTG CCCTTCCTGA ATACCCTTCG GCGGCTAGAA 
GACCGGGCCA CTGCCTATGT GTGCGAGAAT CAGGCCTGCT CGATGCCCAT CACTGAGCCC 
TGCGAACTAC GGAAAGTGCT CCATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001076876.1
CDS	33..2402
Translation	MLGARALRCRGFLLPGAGPVLALSYRGSSARDKDRSVTVSSSVPMPAGGKGSRTNCSQSTPQKVPNRLINEKSPYLLQHAYNPVDWYPWGQEAFDKAKKENKPIFLSVGYSTCHWCHMMEEESFQNEEIGRLLSEDFVSVKVDREERPDVDKVYMTFVQATSSGGGWPMSVWLTPDLQPFVGGTYFPPEDGLTRVGFRTVLMRIRDQWKQNKSTLLENSQRVTTALLARSAISMGDRQLPPSAATMNSRCFQQLDEGYDEEYGGFAEAPKFPTPVILSFLFSYWLSHRLTQDGSRAQQMALHTLKMMANGGIRDHVGQGFHRYSTDRQWHVPHFEKMLYDQAQLTVAYSQAFQISGDEFYSEVAKGILQYVVRNLSHRSGGFYSAEDADSPPERGMRPKEGAFYVWTVKEVQHLLPEPVLGATEPLTSGQLLMKHYGLTEAGNISPSQDPKGELQGQNVLTVRYSLELTAARFGLDVEAVRTLLNSGLEKLFQARKHRPKPHLDSKMLAAWNGLMVSGFAVTGAVLGQERVINYAINGAKFLKRHMFDVASGRLMRTCYAGSGGTVEHSNPPCWGFLEDYAFVVRGLLDLYEASQESAWLEWALRLQDTQDRLFWDSRGGGYFCSEAELGAGLPLRLKDDQDGAEPSANSVSAHNLLRLHGFTGHKDWMDKCVCLLTAFSERMRRVPVALPEMVRALSAHQQTLKQIVICGDPQAKDTKALLQCVHSIYIPNKVLILADGDPSSFLSRQLPFLNTLRRLEDRATAYVCENQACSMPITEPCELRKVLHQ

Target ORF information:

RefSeq Version	NM_001083407.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spermatogenesis associated 20 (SPATA20), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001083407.1

ORF Insert Sequence:

ATGCTGGGCG CGCGGGCCTT ACGGTGCCGC GGCTTCCTGC TGCCCGGGGC CGGGCCAGTC 
CTCGCCTTGA GCTACAGGGG TAGCTCCGCC CGGGACAAGG ACCGAAGTGT GACGGTCAGT 
AGTTCAGTGC CCATGCCTGC TGGAGGGAAG GGGAGCCGGA CTAACTGCTC TCAGTCCACG 
CCCCAGAAGG TCCCCAACCG CCTGATCAAT GAGAAGTCAC CGTACCTCCT GCAACATGCC 
TACAACCCTG TGGACTGGTA CCCCTGGGGA CAGGAAGCCT TCGACAAGGC CAAGAAAGAA 
AACAAGCCAA TTTTTCTTTC AGTGGGGTAC TCCACCTGCC ACTGGTGCCA CATGATGGAG 
GAGGAGTCCT TCCAGAATGA GGAGATTGGC CGCCTGCTCA GCGAGGACTT TGTCAGTGTG 
AAGGTGGACC GGGAGGAGCG GCCTGACGTG GACAAGGTGT ATATGACTTT TGTGCAGGCC 
ACCAGCAGTG GTGGGGGCTG GCCCATGAGT GTGTGGCTGA CTCCCGACCT ACAGCCCTTT 
GTGGGCGGCA CCTATTTTCC CCCTGAGGAT GGCTTGACCC GAGTTGGCTT CCGCACAGTG 
TTGATGAGGA TACGGGACCA GTGGAAACAG AACAAGAGTA CCCTGCTAGA AAACAGTCAG 
CGGGTCACCA CGGCTCTGCT GGCTCGGTCG GCGATCAGTA TGGGTGACCG CCAGTTGCCA 
CCTTCCGCTG CCACCATGAA CAGCCGCTGC TTCCAGCAGC TGGATGAGGG CTATGATGAG 
GAGTATGGTG GCTTCGCTGA GGCCCCCAAG TTCCCCACAC CAGTGATCCT GAGTTTCCTG 
TTCTCCTACT GGCTCAGCCA CCGGCTAACC CAGGACGGCT CTCGGGCCCA GCAGATGGCC 
TTGCATACCC TGAAGATGAT GGCCAACGGG GGTATCCGAG ACCACGTGGG GCAGGGCTTC 
CACCGCTACT CCACGGACCG CCAGTGGCAC GTTCCCCACT TTGAGAAGAT GCTGTATGAC 
CAGGCACAGC TCACGGTGGC CTATTCACAA GCTTTCCAGA TCTCTGGTGA CGAGTTCTAC 
TCCGAAGTTG CCAAAGGTAT CCTGCAGTAC GTCGTTCGGA ACCTGAGTCA CCGGTCTGGA 
GGCTTCTATA GTGCAGAGGA CGCGGACTCC CCGCCGGAGC GGGGCATGCG GCCCAAAGAG 
GGCGCCTTCT ACGTGTGGAC TGTCAAGGAG GTCCAGCATC TCCTCCCTGA GCCTGTGCTG 
GGCGCCACCG AGCCTCTGAC CTCAGGCCAG CTCCTCATGA AGCACTATGG GCTCACGGAG 
GCTGGCAACA TCAGCCCCAG TCAGGACCCC AAGGGGGAGC TGCAGGGCCA GAACGTGCTG 
ACCGTCCGAT ATTCGCTGGA GCTGACTGCC GCCCGCTTTG GCCTGGACGT GGAGGCTGTA 
CGAACCTTAC TCAATTCAGG CCTCGAGAAG CTCTTCCAGG CCCGGAAACA TCGGCCGAAG 
CCGCACTTGG ACAGCAAGAT GCTGGCTGCC TGGAATGGAC TGATGGTGTC TGGCTTTGCT 
GTGACTGGGG CTGTTCTGGG CCAGGAGAGG GTGATCAACT ACGCCATCAA TGGTGCCAAG 
TTCCTGAAGC GGCACATGTT TGATGTGGCC AGTGGCCGCC TGATGCGGAC CTGCTATGCA 
GGCTCTGGTG GGACTGTGGA ACACAGCAAC CCGCCCTGCT GGGGCTTCCT GGAGGACTAT 
GCCTTCGTGG TGAGGGGCCT GCTGGACTTG TACGAGGCCT CACAGGAGAG TGCGTGGCTC 
GAGTGGGCTC TGCGGCTGCA GGACACACAG GACAGGCTCT TCTGGGACTC CCGGGGCGGC 
GGCTACTTCT GCAGTGAGGC CGAGCTGGGG GCTGGCCTGC CGCTGCGTCT GAAGGACGAC 
CAGGATGGTG CAGAGCCCAG TGCCAACTCC GTGTCGGCCC ACAACCTGCT TCGGCTGCAC 
GGTTTCACGG GCCACAAGGA CTGGATGGAC AAGTGTGTGT GCCTGTTGAC CGCCTTCTCT 
GAGCGCATGC GCCGTGTGCC CGTGGCATTG CCTGAGATGG TCCGCGCCCT CTCAGCCCAC 
CAGCAGACCC TCAAGCAGAT TGTGATCTGT GGGGACCCCC AGGCCAAGGA TACCAAGGCT 
CTGTTGCAGT GTGTCCACTC CATCTACATC CCTAACAAGG TGCTGATTCT GGCTGATGGG 
GACCCCTCGA GCTTCCTGTC CCGCCAGCTG CCCTTCCTGA ATACCCTTCG GCGGCTAGAA 
GACCGGGCCA CTGCCTATGT GTGCGAGAAT CAGGCCTGCT CGATGCCCAT CACTGAGCCC 
TGCGAACTAC GGAAAGTGCT CCATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SPATA20 ( XM_024979934.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024835702.1 Bos taurus spermatogenesis associated 20 (SPATA20), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa29891
Clone ID Related Accession (Same CDS sequence)	XM_024979934.1
Accession Version	XM_024979934.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2370bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	spermatogenesis-associated protein 20 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037346.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCCACC TTTCTTCACC CCCCCAAAAA CACAAGGGGG AGCACAAAGG CCACGGTCTC 
CCCCGTGGTT CAGAAAGGGG TAGCTCCGCC CGGGACAAGG ACCGAAGTGT GACGGTCAGT 
AGTTCAGTGC CCATGCCTGC TGGAGGGAAG GGGAGCCGGA CTAACTGCTC TCAGTCCACG 
CCCCAGAAGG TCCCCAACCG CCTGATCAAT GAGAAGTCAC CGTACCTCCT GCAACATGCC 
TACAACCCTG TGGACTGGTA CCCCTGGGGA CAGGAAGCCT TCGACAAGGC CAAGAAAGAA 
AACAAGCCAA TTTTTCTTTC AGTGGGGTAC TCCACCTGCC ACTGGTGCCA CATGATGGAG 
GAGGAGTCCT TCCAGAATGA GGAGATTGGC CGCCTGCTCA GCGAGGACTT TGTCAGTGTG 
AAGGTGGACC GGGAGGAGCG GCCTGACGTG GACAAGGTGT ATATGACTTT TGTGCAGGCC 
ACCAGCAGTG GTGGGGGCTG GCCCATGAGT GTGTGGCTGA CTCCCGACCT ACAGCCCTTT 
GTGGGCGGCA CCTATTTTCC CCCTGAGGAT GGCTTGACCC GAGTTGGCTT CCGCACAGTG 
TTGATGAGGA TACGGGACCA GTGGAAACAG AACAAGAGTA CCCTGCTAGA AAACAGTCAG 
CGGGTCACCA CGGCTCTGCT GGCTCGGTCG GCGATCAGTA TGGGTGACCG CCAGTTGCCA 
CCTTCCGCTG CCACCATGAA CAGCCGCTGC TTCCAGCAGC TGGATGAGGG CTATGATGAG 
GAGTATGGTG GCTTCGCTGA GGCCCCCAAG TTCCCCACAC CAGTGATCCT GAGTTTCCTG 
TTCTCCTACT GGCTCAGCCA CCGGCTAACC CAGGACGGCT CTCGGGCCCA GCAGATGGCC 
TTGCATACCC TGAAGATGAT GGCCAACGGG GGTATCCGAG ACCACGTGGG GCAGGGCTTC 
CACCGCTACT CCACGGACCG CCAGTGGCAC GTTCCCCACT TTGAGAAGAT GCTGTATGAC 
CAGGCACAGC TCACGGTGGC CTATTCACAA GCTTTCCAGA TCTCTGGTGA CGAGTTCTAC 
TCCGAAGTTG CCAAAGGTAT CCTGCAGTAC GTCGTTCGGA ACCTGAGTCA CCGGTCTGGA 
GGCTTCTATA GTGCAGAGGA CGCGGACTCC CCGCCGGAGC GGGGCATGCG GCCCAAAGAG 
GGCGCCTTCT ACGTGTGGAC TGTCAAGGAG GTCCAGCATC TCCTCCCTGA GCCTGTGCTG 
GGCGCCACCG AGCCTCTGAC CTCAGGCCAG CTCCTCATGA AGCACTATGG GCTCACGGAG 
GCTGGCAACA TCAGCCCCAG TCAGGACCCC AAGGGGGAGC TGCAGGGCCA GAACGTGCTG 
ACCGTCCGAT ATTCGCTGGA GCTGACTGCC GCCCGCTTTG GCCTGGACGT GGAGGCTGTA 
CGAACCTTAC TCAATTCAGG CCTCGAGAAG CTCTTCCAGG CCCGGAAACA TCGGCCGAAG 
CCGCACTTGG ACAGCAAGAT GCTGGCTGCC TGGAATGGAC TGATGGTGTC TGGCTTTGCT 
GTGACTGGGG CTGTTCTGGG CCAGGAGAGG GTGATCAACT ACGCCATCAA TGGTGCCAAG 
TTCCTGAAGC GGCACATGTT TGATGTGGCC AGTGGCCGCC TGATGCGGAC CTGCTATGCA 
GGCTCTGGTG GGACTGTGGA ACACAGCAAC CCGCCCTGCT GGGGCTTCCT GGAGGACTAT 
GCCTTCGTGG TGAGGGGCCT GCTGGACTTG TACGAGGCCT CACAGGAGAG TGCGTGGCTC 
GAGTGGGCTC TGCGGCTGCA GGACACACAG GACAGGCTCT TCTGGGACTC CCGGGGCGGC 
GGCTACTTCT GCAGTGAGGC CGAGCTGGGG GCTGGCCTGC CGCTGCGTCT GAAGGACGAC 
CAGGATGGTG CAGAGCCCAG TGCCAACTCC GTGTCGGCCC ACAACCTGCT TCGGCTGCAC 
GGTTTCACGG GCCACAAGGA CTGGATGGAC AAGTGTGTGT GCCTGTTGAC CGCCTTCTCT 
GAGCGCATGC GCCGTGTGCC CGTGGCATTG CCTGAGATGG TCCGCGCCCT CTCAGCCCAC 
CAGCAGACCC TCAAGCAGAT TGTGATCTGT GGGGACCCCC AGGCCAAGGA TACCAAGGCT 
CTGTTGCAGT GTGTCCACTC CATCTACATC CCTAACAAGG TGCTGATTCT GGCTGATGGG 
GACCCCTCGA GCTTCCTGTC CCGCCAGCTG CCCTTCCTGA ATACCCTTCG GCGGCTAGAA 
GACCGGGCCA CTGCCTATGT GTGCGAGAAT CAGGCCTGCT CGATGCCCAT CACTGAGCCC 
TGCGAACTAC GGAAAGTGCT CCATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024835702.1
CDS	73..2442
Translation	MSHLSSPPQKHKGEHKGHGLPRGSERGSSARDKDRSVTVSSSVPMPAGGKGSRTNCSQSTPQKVPNRLINEKSPYLLQHAYNPVDWYPWGQEAFDKAKKENKPIFLSVGYSTCHWCHMMEEESFQNEEIGRLLSEDFVSVKVDREERPDVDKVYMTFVQATSSGGGWPMSVWLTPDLQPFVGGTYFPPEDGLTRVGFRTVLMRIRDQWKQNKSTLLENSQRVTTALLARSAISMGDRQLPPSAATMNSRCFQQLDEGYDEEYGGFAEAPKFPTPVILSFLFSYWLSHRLTQDGSRAQQMALHTLKMMANGGIRDHVGQGFHRYSTDRQWHVPHFEKMLYDQAQLTVAYSQAFQISGDEFYSEVAKGILQYVVRNLSHRSGGFYSAEDADSPPERGMRPKEGAFYVWTVKEVQHLLPEPVLGATEPLTSGQLLMKHYGLTEAGNISPSQDPKGELQGQNVLTVRYSLELTAARFGLDVEAVRTLLNSGLEKLFQARKHRPKPHLDSKMLAAWNGLMVSGFAVTGAVLGQERVINYAINGAKFLKRHMFDVASGRLMRTCYAGSGGTVEHSNPPCWGFLEDYAFVVRGLLDLYEASQESAWLEWALRLQDTQDRLFWDSRGGGYFCSEAELGAGLPLRLKDDQDGAEPSANSVSAHNLLRLHGFTGHKDWMDKCVCLLTAFSERMRRVPVALPEMVRALSAHQQTLKQIVICGDPQAKDTKALLQCVHSIYIPNKVLILADGDPSSFLSRQLPFLNTLRRLEDRATAYVCENQACSMPITEPCELRKVLHQ

Target ORF information:

RefSeq Version	XM_024979934.1
Organism	Bos taurus(cattle)
Definition	Bos taurus spermatogenesis associated 20 (SPATA20), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024979934.1

ORF Insert Sequence:

ATGAGCCACC TTTCTTCACC CCCCCAAAAA CACAAGGGGG AGCACAAAGG CCACGGTCTC 
CCCCGTGGTT CAGAAAGGGG TAGCTCCGCC CGGGACAAGG ACCGAAGTGT GACGGTCAGT 
AGTTCAGTGC CCATGCCTGC TGGAGGGAAG GGGAGCCGGA CTAACTGCTC TCAGTCCACG 
CCCCAGAAGG TCCCCAACCG CCTGATCAAT GAGAAGTCAC CGTACCTCCT GCAACATGCC 
TACAACCCTG TGGACTGGTA CCCCTGGGGA CAGGAAGCCT TCGACAAGGC CAAGAAAGAA 
AACAAGCCAA TTTTTCTTTC AGTGGGGTAC TCCACCTGCC ACTGGTGCCA CATGATGGAG 
GAGGAGTCCT TCCAGAATGA GGAGATTGGC CGCCTGCTCA GCGAGGACTT TGTCAGTGTG 
AAGGTGGACC GGGAGGAGCG GCCTGACGTG GACAAGGTGT ATATGACTTT TGTGCAGGCC 
ACCAGCAGTG GTGGGGGCTG GCCCATGAGT GTGTGGCTGA CTCCCGACCT ACAGCCCTTT 
GTGGGCGGCA CCTATTTTCC CCCTGAGGAT GGCTTGACCC GAGTTGGCTT CCGCACAGTG 
TTGATGAGGA TACGGGACCA GTGGAAACAG AACAAGAGTA CCCTGCTAGA AAACAGTCAG 
CGGGTCACCA CGGCTCTGCT GGCTCGGTCG GCGATCAGTA TGGGTGACCG CCAGTTGCCA 
CCTTCCGCTG CCACCATGAA CAGCCGCTGC TTCCAGCAGC TGGATGAGGG CTATGATGAG 
GAGTATGGTG GCTTCGCTGA GGCCCCCAAG TTCCCCACAC CAGTGATCCT GAGTTTCCTG 
TTCTCCTACT GGCTCAGCCA CCGGCTAACC CAGGACGGCT CTCGGGCCCA GCAGATGGCC 
TTGCATACCC TGAAGATGAT GGCCAACGGG GGTATCCGAG ACCACGTGGG GCAGGGCTTC 
CACCGCTACT CCACGGACCG CCAGTGGCAC GTTCCCCACT TTGAGAAGAT GCTGTATGAC 
CAGGCACAGC TCACGGTGGC CTATTCACAA GCTTTCCAGA TCTCTGGTGA CGAGTTCTAC 
TCCGAAGTTG CCAAAGGTAT CCTGCAGTAC GTCGTTCGGA ACCTGAGTCA CCGGTCTGGA 
GGCTTCTATA GTGCAGAGGA CGCGGACTCC CCGCCGGAGC GGGGCATGCG GCCCAAAGAG 
GGCGCCTTCT ACGTGTGGAC TGTCAAGGAG GTCCAGCATC TCCTCCCTGA GCCTGTGCTG 
GGCGCCACCG AGCCTCTGAC CTCAGGCCAG CTCCTCATGA AGCACTATGG GCTCACGGAG 
GCTGGCAACA TCAGCCCCAG TCAGGACCCC AAGGGGGAGC TGCAGGGCCA GAACGTGCTG 
ACCGTCCGAT ATTCGCTGGA GCTGACTGCC GCCCGCTTTG GCCTGGACGT GGAGGCTGTA 
CGAACCTTAC TCAATTCAGG CCTCGAGAAG CTCTTCCAGG CCCGGAAACA TCGGCCGAAG 
CCGCACTTGG ACAGCAAGAT GCTGGCTGCC TGGAATGGAC TGATGGTGTC TGGCTTTGCT 
GTGACTGGGG CTGTTCTGGG CCAGGAGAGG GTGATCAACT ACGCCATCAA TGGTGCCAAG 
TTCCTGAAGC GGCACATGTT TGATGTGGCC AGTGGCCGCC TGATGCGGAC CTGCTATGCA 
GGCTCTGGTG GGACTGTGGA ACACAGCAAC CCGCCCTGCT GGGGCTTCCT GGAGGACTAT 
GCCTTCGTGG TGAGGGGCCT GCTGGACTTG TACGAGGCCT CACAGGAGAG TGCGTGGCTC 
GAGTGGGCTC TGCGGCTGCA GGACACACAG GACAGGCTCT TCTGGGACTC CCGGGGCGGC 
GGCTACTTCT GCAGTGAGGC CGAGCTGGGG GCTGGCCTGC CGCTGCGTCT GAAGGACGAC 
CAGGATGGTG CAGAGCCCAG TGCCAACTCC GTGTCGGCCC ACAACCTGCT TCGGCTGCAC 
GGTTTCACGG GCCACAAGGA CTGGATGGAC AAGTGTGTGT GCCTGTTGAC CGCCTTCTCT 
GAGCGCATGC GCCGTGTGCC CGTGGCATTG CCTGAGATGG TCCGCGCCCT CTCAGCCCAC 
CAGCAGACCC TCAAGCAGAT TGTGATCTGT GGGGACCCCC AGGCCAAGGA TACCAAGGCT 
CTGTTGCAGT GTGTCCACTC CATCTACATC CCTAACAAGG TGCTGATTCT GGCTGATGGG 
GACCCCTCGA GCTTCCTGTC CCGCCAGCTG CCCTTCCTGA ATACCCTTCG GCGGCTAGAA 
GACCGGGCCA CTGCCTATGT GTGCGAGAAT CAGGCCTGCT CGATGCCCAT CACTGAGCCC 
TGCGAACTAC GGAAAGTGCT CCATCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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SPATA20 cDNA ORF clone, Bos taurus(cattle)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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