tbc1d1 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following tbc1d1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa41912 XM_018095351.1
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
OXa49673 XM_031895363.1
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa49674 XM_031895364.1
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa49675 XM_031895365.1
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa49676 XM_031895366.1
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa49677 XM_004910931.2
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OXa49678 XM_004910932.4
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa49679 XM_004910933.3
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OXa04183 XM_004910931.1
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OXa04184 XM_004910932.3
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa04185 XM_002938655.2
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OXa04186 XM_004910933.2
Latest version!
Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa41912
    Clone ID Related Accession (Same CDS sequence) XM_018095351.1
    Accession Version XM_018095351.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3357bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACC TTTTCTACCG TTCTACTGCT CTTCGAAAGA AACTTCATTC CTCATCTTCT
    GTGCCAAACT TTCTCAAATT TCTGGCCCCT GTAGCGGAAA ATAGCACCGC CGGCTTTAAC
    AGTAGCAAAA GTGACTTTGA ATGTAAGTCA ATACACAGCG AAGCTGGATG TGAGAGCCCA
    GTAAGGACGC GCAGACATTC TTGGCGGCAA CAGATCTTCT TACGTGTAGC CACCCCCCAA
    AAGGGCTGCG ATTCCCCCAA CAGATATGAT GACCACTCAG ATTTGGGGGA CATTGCACCC
    AGATCACCTC TAGAACCTGT TTCTGAAGAT GGTCCAGTCA GGTCGGCAGT TGCAGTAAGG
    CACCGGACAA CAGAAGAGCT TCGAGAGCTC TGGAAAAAGG CAATTCTTCA ACAAATTCTG
    CTTCTCAGGA TGGAAAAAGA AAACCAAAAA CTGCAAGCCT CAGAAAATGA CTTGCAGAAT
    AAGAGGCTGA AACTTGACTA TGAAGAAATC ACTCCATGCC TGAAAGATGT GACTTTAGTT
    TGGGAGAAGA TGCTCAGCAC ACCCGGGAGA TCAAAGATCA AGACTGACAC AGAAAAAATA
    CATGCAGCTG TTGGACAAGG GGTCCCCCGT CAGCACCGTG GGGAGATCTG GAAGTTTTTA
    TCAGAACAGT ACCAGCTGAG ACATCAGCAT ATAAGTAAGA ACCAGTTAAA AGACACCCCG
    TACAAGGAGC TTCTGAAGCA ACTGACCACC CAACAGCATG CAATACTCAT TGATCTAGGA
    CGCACCTTTC CAACACACCC GTACTTCTCG GCCCAGCTGG GGGCCGGACA GCTGTCACTC
    TACAACATTC TAAAGGCCTA CTCCTTGCTG GATCCCGAAG TGGGCTACTG CCAGGGCCTC
    AGCTTTGTCG CTGGCGTCCT TTTGCTTCAC ATGACCGAGG AAGACGCCTT TAAGATGCTA
    AAGTTTCTCA TGTATGACTT GGGTCTGAGG AAGCAGTATC GGCCCCACAT GATAACTCTT
    CAGATCCAGA TGTACCAGCT TTCCCGACTC CTGCACGACT ATCACCGGGA TCTGTACAAT
    CACTTGGAAA CCTATGAGAT CGGGCCCAGC CTGTATGCTG CTCCTTGGTT CCTTACCATG
    TTCGCCTCTC AGTTCCCGCT GGGATTTGTG GCGAGAGTCT TTGATATGAT TTTCCTTCAG
    GGATCAGAAG TCATATTTAA GGTGGCCCTC AGCTTGCTCG GAAGCCACAA GCCCTTGATT
    TTGCAGCATG AAAATTTGGA ATCTATTGTA GAATTTATAA AGAACACTCT TCCAAACTTG
    GGTCTGGTGC AGATGGAAAA GACCATTAAC CAGGTGTTTG AAATGGACAT TTCCAAGCAG
    CTTCAGGCAT ATGAAGTGGA ATATCATGTA CTGCAGGATG AACTGATTGA TTCATCTCCT
    CTCAGTGACA ACCAGAGGAT AAATAAACTA GAGAAGGCAA ATAGTACATT GCGCAAACAG
    AACTTTGACC TTTTGGAGGA GCTGCAGGTA TCACATGGGA GAATACAGGT GCTAGAATCC
    ACCGTAGAAA ACTTACAGAG CAACGAGGTC AAGTTAAAAC GATCCGTCCT TACTCTGGAG
    CTGGAAAGGT CTGCCTTGCT AAATACCATC GAAGAATTAC GTGCTCAGAT TAAAGATATG
    CAAGGGAACA AGCTCTGTAA TGACCAAAAT GAGCCCAACC AGCTCAACAA GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017950840.1
    CDS10..3366
    Translation

    Target ORF information:

    RefSeq Version XM_018095351.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018095351.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACC TTTTCTACCG TTCTACTGCT CTTCGAAAGA AACTTCATTC CTCATCTTCT
    GTGCCAAACT TTCTCAAATT TCTGGCCCCT GTAGCGGAAA ATAGCACCGC CGGCTTTAAC
    AGTAGCAAAA GTGACTTTGA ATGTAAGTCA ATACACAGCG AAGCTGGATG TGAGAGCCCA
    GTAAGGACGC GCAGACATTC TTGGCGGCAA CAGATCTTCT TACGTGTAGC CACCCCCCAA
    AAGGGCTGCG ATTCCCCCAA CAGATATGAT GACCACTCAG ATTTGGGGGA CATTGCACCC
    AGATCACCTC TAGAACCTGT TTCTGAAGAT GGTCCAGTCA GGTCGGCAGT TGCAGTAAGG
    CACCGGACAA CAGAAGAGCT TCGAGAGCTC TGGAAAAAGG CAATTCTTCA ACAAATTCTG
    CTTCTCAGGA TGGAAAAAGA AAACCAAAAA CTGCAAGCCT CAGAAAATGA CTTGCAGAAT
    AAGAGGCTGA AACTTGACTA TGAAGAAATC ACTCCATGCC TGAAAGATGT GACTTTAGTT
    TGGGAGAAGA TGCTCAGCAC ACCCGGGAGA TCAAAGATCA AGACTGACAC AGAAAAAATA
    CATGCAGCTG TTGGACAAGG GGTCCCCCGT CAGCACCGTG GGGAGATCTG GAAGTTTTTA
    TCAGAACAGT ACCAGCTGAG ACATCAGCAT ATAAGTAAGA ACCAGTTAAA AGACACCCCG
    TACAAGGAGC TTCTGAAGCA ACTGACCACC CAACAGCATG CAATACTCAT TGATCTAGGA
    CGCACCTTTC CAACACACCC GTACTTCTCG GCCCAGCTGG GGGCCGGACA GCTGTCACTC
    TACAACATTC TAAAGGCCTA CTCCTTGCTG GATCCCGAAG TGGGCTACTG CCAGGGCCTC
    AGCTTTGTCG CTGGCGTCCT TTTGCTTCAC ATGACCGAGG AAGACGCCTT TAAGATGCTA
    AAGTTTCTCA TGTATGACTT GGGTCTGAGG AAGCAGTATC GGCCCCACAT GATAACTCTT
    CAGATCCAGA TGTACCAGCT TTCCCGACTC CTGCACGACT ATCACCGGGA TCTGTACAAT
    CACTTGGAAA CCTATGAGAT CGGGCCCAGC CTGTATGCTG CTCCTTGGTT CCTTACCATG
    TTCGCCTCTC AGTTCCCGCT GGGATTTGTG GCGAGAGTCT TTGATATGAT TTTCCTTCAG
    GGATCAGAAG TCATATTTAA GGTGGCCCTC AGCTTGCTCG GAAGCCACAA GCCCTTGATT
    TTGCAGCATG AAAATTTGGA ATCTATTGTA GAATTTATAA AGAACACTCT TCCAAACTTG
    GGTCTGGTGC AGATGGAAAA GACCATTAAC CAGGTGTTTG AAATGGACAT TTCCAAGCAG
    CTTCAGGCAT ATGAAGTGGA ATATCATGTA CTGCAGGATG AACTGATTGA TTCATCTCCT
    CTCAGTGACA ACCAGAGGAT AAATAAACTA GAGAAGGCAA ATAGTACATT GCGCAAACAG
    AACTTTGACC TTTTGGAGGA GCTGCAGGTA TCACATGGGA GAATACAGGT GCTAGAATCC
    ACCGTAGAAA ACTTACAGAG CAACGAGGTC AAGTTAAAAC GATCCGTCCT TACTCTGGAG
    CTGGAAAGGT CTGCCTTGCT AAATACCATC GAAGAATTAC GTGCTCAGAT TAAAGATATG
    CAAGGGAACA AGCTCTGTAA TGACCAAAAT GAGCCCAACC AGCTCAACAA GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49673
    Clone ID Related Accession (Same CDS sequence) XM_031895363.1
    Accession Version XM_031895363.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3861bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AACACTCCTC TGCCTGAGCC GGATCACAAT CCAGTGCAGT CTGCAGAACC GACTGCTCCT
    CGTCCCGTCT CTGCAACCTC TTCCCCTGTA ACTGGGAAAA AACTGAAGGT CGGTTCTGTT
    GGGGAAGCAG GGGCGCCACT TTCACAAAAG AGCCTTTTCT ACCGTTCTAC TGCTCTTCGA
    AAGAAACTTC ATTCCTCATC TTCTGTGCCA AACTTTCTCA AATTTCTGGC CCCTGTAGCG
    GAAAATAGCA CCGCCGGCTT TAACAGTAGC AAAAGTGACT TTGAATGTAA GTCAATACAC
    AGCGAAGCTG GATGTGAGAG CCCAGTAAGG ACGCGCAGAC ATTCTTGGCG GCAACAGATC
    TTCTTACGTG TAGCCACCCC CCAAAAGGGC TGCGATTCCC CCAACAGATA TGATGACCAC
    TCAGATTTGG GGGACATTGC ACCCAGATCA CCTCTAGAAC CTGTTTCTGA AGATGGTCCA
    GTCAGGTCGG CAGTTGCAGT AAGGCACCGG ACAACAGAAG AGCTTCGAGA GCTCTGGAAA
    AAGGCAATTC TTCAACAAAT TCTGCTTCTC AGGATGGAAA AAGAAAACCA AAAACTGCAA
    GCCTCAGAAA ATGACTTGCA GAATAAGAGG CTGAAACTTG ACTATGAAGA AATCACTCCA
    TGCCTGAAAG ATGTGACTTT AGTTTGGGAG AAGATGCTCA GCACACCCGG GAGATCAAAG
    ATCAAGACTG ACACAGAAAA AATACATGCA GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC
    CGTGGGGAGA TCTGGAAGTT TTTATCAGAA CAGTACCAGC TGAGACATCA GCATATAAGT
    AAGAACCAGT TAAAAGACAC CCCGTACAAG GAGCTTCTGA AGCAACTGAC CACCCAACAG
    CATGCAATAC TCATTGATCT AGGACGCACC TTTCCAACAC ACCCGTACTT CTCGGCCCAG
    CTGGGGGCCG GACAGCTGTC ACTCTACAAC ATTCTAAAGG CCTACTCCTT GCTGGATCCC
    GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT GTCGCTGGCG TCCTTTTGCT TCACATGACC
    GAGGAAGACG CCTTTAAGAT GCTAAAGTTT CTCATGTATG ACTTGGGTCT GAGGAAGCAG
    TATCGGCCCG ACATGATAAC TCTTCAGATC CAGATGTACC AGCTTTCCCG ACTCCTGCAC
    GACTATCACC GGGATCTGTA CAATCACTTG GAAACCTATG AGATCGGGCC CAGCCTGTAT
    GCTGCTCCTT GGTTCCTTAC CATGTTCGCC TCTCAGTTCC CGCTGGGATT TGTGGCGAGA
    GTCTTTGATA TGATTTTCCT TCAGGGATCA GAAGTCATAT TTAAGGTGGC CCTCAGCTTG
    CTCGGAAGCC ACAAGCCCTT GATTTTGCAG CATGAAAATT TGGAATCTAT TGTAGAATTT
    ATAAAGAACA CTCTTCCAAA CTTGGGTCTG GTGCAGATGG AAAAGACCAT TAACCAGGTG
    TTTGAAATGG ACATTTCCAA GCAGCTTCAG GCATATGAAG TGGAATATCA TGTACTGCAG
    GATGAACTGA TTGATTCATC TCCTCTCAGT GACAACCAGA GGATAAATAA ACTAGAGAAG
    GCAAATAGTA CATTGCGCAA ACAGAACTTT GACCTTTTGG AGGAGCTGCA GGTATCACAT
    GGGAGAATAC AGGTGCTAGA ATCCACCGTA GAAAACTTAC AGAGCAACGA GGTCAAGTTA
    AAACGATCCG TCCTTACTCT GGAGCTGGAA AGGTCTGCCT TGCTAAATAC CATCGAAGAA
    TTACGTGCTC AGATTAAAGA TATGCAAGGG AACAAGCTCT GTAATGACCA AAATGAGCCC
    AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751223.1
    CDS249..4109
    Translation

    Target ORF information:

    RefSeq Version XM_031895363.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895363.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AACACTCCTC TGCCTGAGCC GGATCACAAT CCAGTGCAGT CTGCAGAACC GACTGCTCCT
    CGTCCCGTCT CTGCAACCTC TTCCCCTGTA ACTGGGAAAA AACTGAAGGT CGGTTCTGTT
    GGGGAAGCAG GGGCGCCACT TTCACAAAAG AGCCTTTTCT ACCGTTCTAC TGCTCTTCGA
    AAGAAACTTC ATTCCTCATC TTCTGTGCCA AACTTTCTCA AATTTCTGGC CCCTGTAGCG
    GAAAATAGCA CCGCCGGCTT TAACAGTAGC AAAAGTGACT TTGAATGTAA GTCAATACAC
    AGCGAAGCTG GATGTGAGAG CCCAGTAAGG ACGCGCAGAC ATTCTTGGCG GCAACAGATC
    TTCTTACGTG TAGCCACCCC CCAAAAGGGC TGCGATTCCC CCAACAGATA TGATGACCAC
    TCAGATTTGG GGGACATTGC ACCCAGATCA CCTCTAGAAC CTGTTTCTGA AGATGGTCCA
    GTCAGGTCGG CAGTTGCAGT AAGGCACCGG ACAACAGAAG AGCTTCGAGA GCTCTGGAAA
    AAGGCAATTC TTCAACAAAT TCTGCTTCTC AGGATGGAAA AAGAAAACCA AAAACTGCAA
    GCCTCAGAAA ATGACTTGCA GAATAAGAGG CTGAAACTTG ACTATGAAGA AATCACTCCA
    TGCCTGAAAG ATGTGACTTT AGTTTGGGAG AAGATGCTCA GCACACCCGG GAGATCAAAG
    ATCAAGACTG ACACAGAAAA AATACATGCA GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC
    CGTGGGGAGA TCTGGAAGTT TTTATCAGAA CAGTACCAGC TGAGACATCA GCATATAAGT
    AAGAACCAGT TAAAAGACAC CCCGTACAAG GAGCTTCTGA AGCAACTGAC CACCCAACAG
    CATGCAATAC TCATTGATCT AGGACGCACC TTTCCAACAC ACCCGTACTT CTCGGCCCAG
    CTGGGGGCCG GACAGCTGTC ACTCTACAAC ATTCTAAAGG CCTACTCCTT GCTGGATCCC
    GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT GTCGCTGGCG TCCTTTTGCT TCACATGACC
    GAGGAAGACG CCTTTAAGAT GCTAAAGTTT CTCATGTATG ACTTGGGTCT GAGGAAGCAG
    TATCGGCCCG ACATGATAAC TCTTCAGATC CAGATGTACC AGCTTTCCCG ACTCCTGCAC
    GACTATCACC GGGATCTGTA CAATCACTTG GAAACCTATG AGATCGGGCC CAGCCTGTAT
    GCTGCTCCTT GGTTCCTTAC CATGTTCGCC TCTCAGTTCC CGCTGGGATT TGTGGCGAGA
    GTCTTTGATA TGATTTTCCT TCAGGGATCA GAAGTCATAT TTAAGGTGGC CCTCAGCTTG
    CTCGGAAGCC ACAAGCCCTT GATTTTGCAG CATGAAAATT TGGAATCTAT TGTAGAATTT
    ATAAAGAACA CTCTTCCAAA CTTGGGTCTG GTGCAGATGG AAAAGACCAT TAACCAGGTG
    TTTGAAATGG ACATTTCCAA GCAGCTTCAG GCATATGAAG TGGAATATCA TGTACTGCAG
    GATGAACTGA TTGATTCATC TCCTCTCAGT GACAACCAGA GGATAAATAA ACTAGAGAAG
    GCAAATAGTA CATTGCGCAA ACAGAACTTT GACCTTTTGG AGGAGCTGCA GGTATCACAT
    GGGAGAATAC AGGTGCTAGA ATCCACCGTA GAAAACTTAC AGAGCAACGA GGTCAAGTTA
    AAACGATCCG TCCTTACTCT GGAGCTGGAA AGGTCTGCCT TGCTAAATAC CATCGAAGAA
    TTACGTGCTC AGATTAAAGA TATGCAAGGG AACAAGCTCT GTAATGACCA AAATGAGCCC
    AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49674
    Clone ID Related Accession (Same CDS sequence) XM_031895364.1
    Accession Version XM_031895364.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AACACTCCTC TGCCTGAGCC GGATCACAAT CCAGTGCAGT CTGCAGAACC GACTGCTCCT
    CGTCCCGTCT CTGCAACCTC TTCCCCTGTA ACTGGGAAAA AACTGAAGGT CGGTTCTGTT
    GGGGAAGCAG GGGCGCCACT TTCACAAAAG AGTGACTTTG AATGTAAGTC AATACACAGC
    GAAGCTGGAT GTGAGAGCCC AGTAAGGACG CGCAGACATT CTTGGCGGCA ACAGATCTTC
    TTACGTGTAG CCACCCCCCA AAAGGGCTGC GATTCCCCCA ACAGATATGA TGACCACTCA
    GATTTGGGGG ACATTGCACC CAGATCACCT CTAGAACCTG TTTCTGAAGA TGGTCCAGTC
    AGGTCGGCAG TTGCAGTAAG GCACCGGACA ACAGAAGAGC TTCGAGAGCT CTGGAAAAAG
    GCAATTCTTC AACAAATTCT GCTTCTCAGG ATGGAAAAAG AAAACCAAAA ACTGCAAGCC
    TCAGAAAATG ACTTGCAGAA TAAGAGGCTG AAACTTGACT ATGAAGAAAT CACTCCATGC
    CTGAAAGATG TGACTTTAGT TTGGGAGAAG ATGCTCAGCA CACCCGGGAG ATCAAAGATC
    AAGACTGACA CAGAAAAAAT ACATGCAGCT GTTGGACAAG GGGTCCCCCG TCAGCACCGT
    GGGGAGATCT GGAAGTTTTT ATCAGAACAG TACCAGCTGA GACATCAGCA TATAAGTAAG
    AACCAGTTAA AAGACACCCC GTACAAGGAG CTTCTGAAGC AACTGACCAC CCAACAGCAT
    GCAATACTCA TTGATCTAGG ACGCACCTTT CCAACACACC CGTACTTCTC GGCCCAGCTG
    GGGGCCGGAC AGCTGTCACT CTACAACATT CTAAAGGCCT ACTCCTTGCT GGATCCCGAA
    GTGGGCTACT GCCAGGGCCT CAGCTTTGTC GCTGGCGTCC TTTTGCTTCA CATGACCGAG
    GAAGACGCCT TTAAGATGCT AAAGTTTCTC ATGTATGACT TGGGTCTGAG GAAGCAGTAT
    CGGCCCGACA TGATAACTCT TCAGATCCAG ATGTACCAGC TTTCCCGACT CCTGCACGAC
    TATCACCGGG ATCTGTACAA TCACTTGGAA ACCTATGAGA TCGGGCCCAG CCTGTATGCT
    GCTCCTTGGT TCCTTACCAT GTTCGCCTCT CAGTTCCCGC TGGGATTTGT GGCGAGAGTC
    TTTGATATGA TTTTCCTTCA GGGATCAGAA GTCATATTTA AGGTGGCCCT CAGCTTGCTC
    GGAAGCCACA AGCCCTTGAT TTTGCAGCAT GAAAATTTGG AATCTATTGT AGAATTTATA
    AAGAACACTC TTCCAAACTT GGGTCTGGTG CAGATGGAAA AGACCATTAA CCAGGTGTTT
    GAAATGGACA TTTCCAAGCA GCTTCAGGCA TATGAAGTGG AATATCATGT ACTGCAGGAT
    GAACTGATTG ATTCATCTCC TCTCAGTGAC AACCAGAGGA TAAATAAACT AGAGAAGGCA
    AATAGTACAT TGCGCAAACA GAACTTTGAC CTTTTGGAGG AGCTGCAGGT ATCACATGGG
    AGAATACAGG TGCTAGAATC CACCGTAGAA AACTTACAGA GCAACGAGGT CAAGTTAAAA
    CGATCCGTCC TTACTCTGGA GCTGGAAAGG TCTGCCTTGC TAAATACCAT CGAAGAATTA
    CGTGCTCAGA TTAAAGATAT GCAAGGGAAC AAGCTCTGTA ATGACCAAAA TGAGCCCAAC
    CAGCTCAACA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751224.1
    CDS247..3984
    Translation

    Target ORF information:

    RefSeq Version XM_031895364.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895364.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AACACTCCTC TGCCTGAGCC GGATCACAAT CCAGTGCAGT CTGCAGAACC GACTGCTCCT
    CGTCCCGTCT CTGCAACCTC TTCCCCTGTA ACTGGGAAAA AACTGAAGGT CGGTTCTGTT
    GGGGAAGCAG GGGCGCCACT TTCACAAAAG AGTGACTTTG AATGTAAGTC AATACACAGC
    GAAGCTGGAT GTGAGAGCCC AGTAAGGACG CGCAGACATT CTTGGCGGCA ACAGATCTTC
    TTACGTGTAG CCACCCCCCA AAAGGGCTGC GATTCCCCCA ACAGATATGA TGACCACTCA
    GATTTGGGGG ACATTGCACC CAGATCACCT CTAGAACCTG TTTCTGAAGA TGGTCCAGTC
    AGGTCGGCAG TTGCAGTAAG GCACCGGACA ACAGAAGAGC TTCGAGAGCT CTGGAAAAAG
    GCAATTCTTC AACAAATTCT GCTTCTCAGG ATGGAAAAAG AAAACCAAAA ACTGCAAGCC
    TCAGAAAATG ACTTGCAGAA TAAGAGGCTG AAACTTGACT ATGAAGAAAT CACTCCATGC
    CTGAAAGATG TGACTTTAGT TTGGGAGAAG ATGCTCAGCA CACCCGGGAG ATCAAAGATC
    AAGACTGACA CAGAAAAAAT ACATGCAGCT GTTGGACAAG GGGTCCCCCG TCAGCACCGT
    GGGGAGATCT GGAAGTTTTT ATCAGAACAG TACCAGCTGA GACATCAGCA TATAAGTAAG
    AACCAGTTAA AAGACACCCC GTACAAGGAG CTTCTGAAGC AACTGACCAC CCAACAGCAT
    GCAATACTCA TTGATCTAGG ACGCACCTTT CCAACACACC CGTACTTCTC GGCCCAGCTG
    GGGGCCGGAC AGCTGTCACT CTACAACATT CTAAAGGCCT ACTCCTTGCT GGATCCCGAA
    GTGGGCTACT GCCAGGGCCT CAGCTTTGTC GCTGGCGTCC TTTTGCTTCA CATGACCGAG
    GAAGACGCCT TTAAGATGCT AAAGTTTCTC ATGTATGACT TGGGTCTGAG GAAGCAGTAT
    CGGCCCGACA TGATAACTCT TCAGATCCAG ATGTACCAGC TTTCCCGACT CCTGCACGAC
    TATCACCGGG ATCTGTACAA TCACTTGGAA ACCTATGAGA TCGGGCCCAG CCTGTATGCT
    GCTCCTTGGT TCCTTACCAT GTTCGCCTCT CAGTTCCCGC TGGGATTTGT GGCGAGAGTC
    TTTGATATGA TTTTCCTTCA GGGATCAGAA GTCATATTTA AGGTGGCCCT CAGCTTGCTC
    GGAAGCCACA AGCCCTTGAT TTTGCAGCAT GAAAATTTGG AATCTATTGT AGAATTTATA
    AAGAACACTC TTCCAAACTT GGGTCTGGTG CAGATGGAAA AGACCATTAA CCAGGTGTTT
    GAAATGGACA TTTCCAAGCA GCTTCAGGCA TATGAAGTGG AATATCATGT ACTGCAGGAT
    GAACTGATTG ATTCATCTCC TCTCAGTGAC AACCAGAGGA TAAATAAACT AGAGAAGGCA
    AATAGTACAT TGCGCAAACA GAACTTTGAC CTTTTGGAGG AGCTGCAGGT ATCACATGGG
    AGAATACAGG TGCTAGAATC CACCGTAGAA AACTTACAGA GCAACGAGGT CAAGTTAAAA
    CGATCCGTCC TTACTCTGGA GCTGGAAAGG TCTGCCTTGC TAAATACCAT CGAAGAATTA
    CGTGCTCAGA TTAAAGATAT GCAAGGGAAC AAGCTCTGTA ATGACCAAAA TGAGCCCAAC
    CAGCTCAACA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49675
    Clone ID Related Accession (Same CDS sequence) XM_031895365.1
    Accession Version XM_031895365.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AACCTTTTCT ACCGTTCTAC TGCTCTTCGA AAGAAACTTC ATTCCTCATC TTCTGTGCCA
    AACTTTCTCA AATTTCTGGC CCCTGTAGCG GAAAATAGCA CCGCCGGCTT TAACAGTAGC
    AAAAGTGACT TTGAATGTAA GTCAATACAC AGCGAAGCTG GATGTGAGAG CCCAGTAAGG
    ACGCGCAGAC ATTCTTGGCG GCAACAGATC TTCTTACGTG TAGCCACCCC CCAAAAGGGC
    TGCGATTCCC CCAACAGATA TGATGACCAC TCAGATTTGG GGGACATTGC ACCCAGATCA
    CCTCTAGAAC CTGTTTCTGA AGATGGTCCA GTCAGGTCGG CAGTTGCAGT AAGGCACCGG
    ACAACAGAAG AGCTTCGAGA GCTCTGGAAA AAGGCAATTC TTCAACAAAT TCTGCTTCTC
    AGGATGGAAA AAGAAAACCA AAAACTGCAA GCCTCAGAAA ATGACTTGCA GAATAAGAGG
    CTGAAACTTG ACTATGAAGA AATCACTCCA TGCCTGAAAG ATGTGACTTT AGTTTGGGAG
    AAGATGCTCA GCACACCCGG GAGATCAAAG ATCAAGACTG ACACAGAAAA AATACATGCA
    GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC CGTGGGGAGA TCTGGAAGTT TTTATCAGAA
    CAGTACCAGC TGAGACATCA GCATATAAGT AAGAACCAGT TAAAAGACAC CCCGTACAAG
    GAGCTTCTGA AGCAACTGAC CACCCAACAG CATGCAATAC TCATTGATCT AGGACGCACC
    TTTCCAACAC ACCCGTACTT CTCGGCCCAG CTGGGGGCCG GACAGCTGTC ACTCTACAAC
    ATTCTAAAGG CCTACTCCTT GCTGGATCCC GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT
    GTCGCTGGCG TCCTTTTGCT TCACATGACC GAGGAAGACG CCTTTAAGAT GCTAAAGTTT
    CTCATGTATG ACTTGGGTCT GAGGAAGCAG TATCGGCCCG ACATGATAAC TCTTCAGATC
    CAGATGTACC AGCTTTCCCG ACTCCTGCAC GACTATCACC GGGATCTGTA CAATCACTTG
    GAAACCTATG AGATCGGGCC CAGCCTGTAT GCTGCTCCTT GGTTCCTTAC CATGTTCGCC
    TCTCAGTTCC CGCTGGGATT TGTGGCGAGA GTCTTTGATA TGATTTTCCT TCAGGGATCA
    GAAGTCATAT TTAAGGTGGC CCTCAGCTTG CTCGGAAGCC ACAAGCCCTT GATTTTGCAG
    CATGAAAATT TGGAATCTAT TGTAGAATTT ATAAAGAACA CTCTTCCAAA CTTGGGTCTG
    GTGCAGATGG AAAAGACCAT TAACCAGGTG TTTGAAATGG ACATTTCCAA GCAGCTTCAG
    GCATATGAAG TGGAATATCA TGTACTGCAG GATGAACTGA TTGATTCATC TCCTCTCAGT
    GACAACCAGA GGATAAATAA ACTAGAGAAG GCAAATAGTA CATTGCGCAA ACAGAACTTT
    GACCTTTTGG AGGAGCTGCA GGTATCACAT GGGAGAATAC AGGTGCTAGA ATCCACCGTA
    GAAAACTTAC AGAGCAACGA GGTCAAGTTA AAACGATCCG TCCTTACTCT GGAGCTGGAA
    AGGTCTGCCT TGCTAAATAC CATCGAAGAA TTACGTGCTC AGATTAAAGA TATGCAAGGG
    AACAAGCTCT GTAATGACCA AAATGAGCCC AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751225.1
    CDS247..3957
    Translation

    Target ORF information:

    RefSeq Version XM_031895365.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895365.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AACCTTTTCT ACCGTTCTAC TGCTCTTCGA AAGAAACTTC ATTCCTCATC TTCTGTGCCA
    AACTTTCTCA AATTTCTGGC CCCTGTAGCG GAAAATAGCA CCGCCGGCTT TAACAGTAGC
    AAAAGTGACT TTGAATGTAA GTCAATACAC AGCGAAGCTG GATGTGAGAG CCCAGTAAGG
    ACGCGCAGAC ATTCTTGGCG GCAACAGATC TTCTTACGTG TAGCCACCCC CCAAAAGGGC
    TGCGATTCCC CCAACAGATA TGATGACCAC TCAGATTTGG GGGACATTGC ACCCAGATCA
    CCTCTAGAAC CTGTTTCTGA AGATGGTCCA GTCAGGTCGG CAGTTGCAGT AAGGCACCGG
    ACAACAGAAG AGCTTCGAGA GCTCTGGAAA AAGGCAATTC TTCAACAAAT TCTGCTTCTC
    AGGATGGAAA AAGAAAACCA AAAACTGCAA GCCTCAGAAA ATGACTTGCA GAATAAGAGG
    CTGAAACTTG ACTATGAAGA AATCACTCCA TGCCTGAAAG ATGTGACTTT AGTTTGGGAG
    AAGATGCTCA GCACACCCGG GAGATCAAAG ATCAAGACTG ACACAGAAAA AATACATGCA
    GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC CGTGGGGAGA TCTGGAAGTT TTTATCAGAA
    CAGTACCAGC TGAGACATCA GCATATAAGT AAGAACCAGT TAAAAGACAC CCCGTACAAG
    GAGCTTCTGA AGCAACTGAC CACCCAACAG CATGCAATAC TCATTGATCT AGGACGCACC
    TTTCCAACAC ACCCGTACTT CTCGGCCCAG CTGGGGGCCG GACAGCTGTC ACTCTACAAC
    ATTCTAAAGG CCTACTCCTT GCTGGATCCC GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT
    GTCGCTGGCG TCCTTTTGCT TCACATGACC GAGGAAGACG CCTTTAAGAT GCTAAAGTTT
    CTCATGTATG ACTTGGGTCT GAGGAAGCAG TATCGGCCCG ACATGATAAC TCTTCAGATC
    CAGATGTACC AGCTTTCCCG ACTCCTGCAC GACTATCACC GGGATCTGTA CAATCACTTG
    GAAACCTATG AGATCGGGCC CAGCCTGTAT GCTGCTCCTT GGTTCCTTAC CATGTTCGCC
    TCTCAGTTCC CGCTGGGATT TGTGGCGAGA GTCTTTGATA TGATTTTCCT TCAGGGATCA
    GAAGTCATAT TTAAGGTGGC CCTCAGCTTG CTCGGAAGCC ACAAGCCCTT GATTTTGCAG
    CATGAAAATT TGGAATCTAT TGTAGAATTT ATAAAGAACA CTCTTCCAAA CTTGGGTCTG
    GTGCAGATGG AAAAGACCAT TAACCAGGTG TTTGAAATGG ACATTTCCAA GCAGCTTCAG
    GCATATGAAG TGGAATATCA TGTACTGCAG GATGAACTGA TTGATTCATC TCCTCTCAGT
    GACAACCAGA GGATAAATAA ACTAGAGAAG GCAAATAGTA CATTGCGCAA ACAGAACTTT
    GACCTTTTGG AGGAGCTGCA GGTATCACAT GGGAGAATAC AGGTGCTAGA ATCCACCGTA
    GAAAACTTAC AGAGCAACGA GGTCAAGTTA AAACGATCCG TCCTTACTCT GGAGCTGGAA
    AGGTCTGCCT TGCTAAATAC CATCGAAGAA TTACGTGCTC AGATTAAAGA TATGCAAGGG
    AACAAGCTCT GTAATGACCA AAATGAGCCC AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49676
    Clone ID Related Accession (Same CDS sequence) XM_031895366.1
    Accession Version XM_031895366.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3588bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AATGACTTTG AATGTAAGTC AATACACAGC GAAGCTGGAT GTGAGAGCCC AGTAAGGACG
    CGCAGACATT CTTGGCGGCA ACAGATCTTC TTACGTGTAG CCACCCCCCA AAAGGGCTGC
    GATTCCCCCA ACAGATATGA TGACCACTCA GATTTGGGGG ACATTGCACC CAGATCACCT
    CTAGAACCTG TTTCTGAAGA TGGTCCAGTC AGGTCGGCAG TTGCAGTAAG GCACCGGACA
    ACAGAAGAGC TTCGAGAGCT CTGGAAAAAG GCAATTCTTC AACAAATTCT GCTTCTCAGG
    ATGGAAAAAG AAAACCAAAA ACTGCAAGCC TCAGAAAATG ACTTGCAGAA TAAGAGGCTG
    AAACTTGACT ATGAAGAAAT CACTCCATGC CTGAAAGATG TGACTTTAGT TTGGGAGAAG
    ATGCTCAGCA CACCCGGGAG ATCAAAGATC AAGACTGACA CAGAAAAAAT ACATGCAGCT
    GTTGGACAAG GGGTCCCCCG TCAGCACCGT GGGGAGATCT GGAAGTTTTT ATCAGAACAG
    TACCAGCTGA GACATCAGCA TATAAGTAAG AACCAGTTAA AAGACACCCC GTACAAGGAG
    CTTCTGAAGC AACTGACCAC CCAACAGCAT GCAATACTCA TTGATCTAGG ACGCACCTTT
    CCAACACACC CGTACTTCTC GGCCCAGCTG GGGGCCGGAC AGCTGTCACT CTACAACATT
    CTAAAGGCCT ACTCCTTGCT GGATCCCGAA GTGGGCTACT GCCAGGGCCT CAGCTTTGTC
    GCTGGCGTCC TTTTGCTTCA CATGACCGAG GAAGACGCCT TTAAGATGCT AAAGTTTCTC
    ATGTATGACT TGGGTCTGAG GAAGCAGTAT CGGCCCGACA TGATAACTCT TCAGATCCAG
    ATGTACCAGC TTTCCCGACT CCTGCACGAC TATCACCGGG ATCTGTACAA TCACTTGGAA
    ACCTATGAGA TCGGGCCCAG CCTGTATGCT GCTCCTTGGT TCCTTACCAT GTTCGCCTCT
    CAGTTCCCGC TGGGATTTGT GGCGAGAGTC TTTGATATGA TTTTCCTTCA GGGATCAGAA
    GTCATATTTA AGGTGGCCCT CAGCTTGCTC GGAAGCCACA AGCCCTTGAT TTTGCAGCAT
    GAAAATTTGG AATCTATTGT AGAATTTATA AAGAACACTC TTCCAAACTT GGGTCTGGTG
    CAGATGGAAA AGACCATTAA CCAGGTGTTT GAAATGGACA TTTCCAAGCA GCTTCAGGCA
    TATGAAGTGG AATATCATGT ACTGCAGGAT GAACTGATTG ATTCATCTCC TCTCAGTGAC
    AACCAGAGGA TAAATAAACT AGAGAAGGCA AATAGTACAT TGCGCAAACA GAACTTTGAC
    CTTTTGGAGG AGCTGCAGGT ATCACATGGG AGAATACAGG TGCTAGAATC CACCGTAGAA
    AACTTACAGA GCAACGAGGT CAAGTTAAAA CGATCCGTCC TTACTCTGGA GCTGGAAAGG
    TCTGCCTTGC TAAATACCAT CGAAGAATTA CGTGCTCAGA TTAAAGATAT GCAAGGGAAC
    AAGCTCTGTA ATGACCAAAA TGAGCCCAAC CAGCTCAACA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031751226.1
    CDS242..3829
    Translation

    Target ORF information:

    RefSeq Version XM_031895366.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031895366.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    ATGGATTCTG TAACATGTAT TACTCCTAAA TCTCCTGGAG CCAAACAGGC TGTGCTGGAA 
    TTTAGTCTTC AGCTGATTGG CTCTTTAACA GTGAACTCGC TGACTACTAT GCCAATGTTA
    CCGTGGGTAG TGGCCGAAAT CAAAAAAAGA AATTCAAAGA CTGTCAAGAA TTTGCAAAAT
    GCCCTCCAGA TTCGCCTGTA CGTGTCCTCC ACAAGCATCC TGTGTGAGCC AGATCCAGGC
    AAGTGCCAAC AATGGGATCC TTTAATTTGC TCCAGCATCT TTGACTGCAA GCCCCAGCTG
    GTCCAGAAAC TAATCCACAA CAGCCAAGAC CCCAGTTATT TTGCATGTCT CCTAAGGGAA
    GAGAACCTTG CAAGCCAAGA AAGCACCTGT TACCTTTTTC ATTCTGCTGA TCCCTTAAAA
    GTGCCGGAAA TAATTGGCTC TATCCGGCAT GCAGGAAAGT TGGCACGGCA GGAAGAGGTT
    CATTCCCAGC TTTGTGATTT GGACGATTCA TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT
    GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA CCCCCTGCTT TGATAGACGA GTGCATTGAC
    AAGTTCAACC ATGTTAGCTG TACTAAAAAG AAGGAAGGGG ACTCTCCGAC TCCACAGGGG
    GACTCTCCAC CTCCACAACC GGACTCCGGC ACTGGGATAT CTTTCGGCGA TAAACTGCGC
    TCAGTCTTCC AATCGGCATT TCACGATGAT GACAAGAATA AGCCCATCCG CAAATCCTAC
    TCCCAGCCAG GCCTGCGGAC TAATACCTCT TTTACAAATG AATGGAAAGA TGGACGCAGT
    AGCCTTCCTA CTTCATTTGA GGAAAGCGAT ATAAGTTTGC ATGTACAAAG GTTATGTGGG
    GAGGAACAGG ACAGTGTGCA GCCAACAGAC ATGGAGGAAA ACCAGACTAT GCTATTCACG
    ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC CCAGACACAA AGAGGATTGT GATTGAAAAG
    AGCTTTAAGG AGATATCATT TTGTTCTCAG GGAATCCAGC ATGCTGACCA CTTTGGTTTT
    ATCTGCAGGG AGCCATTGCT GAATGGAGGG TGCCATTTTG TGTGTTATGT ATTTCAGTGT
    GCAAATGGAG CACTGGTGGA TGAGATCATG CTGACTCTGA AGCAGGCCTT CAGTGTAGCT
    GCAGTGCAGC AGAACTCCAA GACTCTTCCG CAGCTCTGCG ATACTTGTCC CATGCAGTGC
    ATGCATAAAC TCTGTGAGAG AATAGAAGGT CTTCCTCCTA CCAAAATAAA ACTTGAACTG
    CAGAAGCACA TCACAACACT AAGCAATCAG GAGCAGGCCT CCATGTTTGA AGAAATACAG
    AAAATCCGGC CACGGTCAGA GCAGCAAGAA AATGAACTAG TCATCTCTCG CCTTCGATGT
    CTGTATGAGG AGAAACAGAG AGACCATATC CATAACGCTG AAACAAAGCA GAATTCGCAA
    GCAGCCGAAA GCACAGCCAA TGGGCTGCAG ACCAGTGCAT CTCGATTCAA ACTTGAGTCT
    CTGACCAGCA AAGCAAAGAG ATCTTTGACG GAATCTTTAG AAAACATTTT GTCAAGGGGC
    AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT GCCAGTGGGG ATGATGACAG CTCACTATGC
    ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG TCATTATGTG AGACAGAACC GGCTGCTCCG
    CTTGCAATCC CCTTAAAGAC TCACGGCTCC CTTGGAGATT TATCGAAGAG CTCAGATAGC
    TATTCCTGTG AGCATCCGGA GGAATTAGTT CAGCAGGGAT TCCGCCGGCG AGCTAACACA
    TTAAGCCATT TCCCTGTCAC AGCGCAGGAA TTTCCCGAGC CGGTTCAGAT AACTCCATCT
    GTTTCACAGC GGAAGCTAAT AAGGCACCAC TCTGTGAGCA CCGAGACGCC GCACAAAAGA
    AATGACTTTG AATGTAAGTC AATACACAGC GAAGCTGGAT GTGAGAGCCC AGTAAGGACG
    CGCAGACATT CTTGGCGGCA ACAGATCTTC TTACGTGTAG CCACCCCCCA AAAGGGCTGC
    GATTCCCCCA ACAGATATGA TGACCACTCA GATTTGGGGG ACATTGCACC CAGATCACCT
    CTAGAACCTG TTTCTGAAGA TGGTCCAGTC AGGTCGGCAG TTGCAGTAAG GCACCGGACA
    ACAGAAGAGC TTCGAGAGCT CTGGAAAAAG GCAATTCTTC AACAAATTCT GCTTCTCAGG
    ATGGAAAAAG AAAACCAAAA ACTGCAAGCC TCAGAAAATG ACTTGCAGAA TAAGAGGCTG
    AAACTTGACT ATGAAGAAAT CACTCCATGC CTGAAAGATG TGACTTTAGT TTGGGAGAAG
    ATGCTCAGCA CACCCGGGAG ATCAAAGATC AAGACTGACA CAGAAAAAAT ACATGCAGCT
    GTTGGACAAG GGGTCCCCCG TCAGCACCGT GGGGAGATCT GGAAGTTTTT ATCAGAACAG
    TACCAGCTGA GACATCAGCA TATAAGTAAG AACCAGTTAA AAGACACCCC GTACAAGGAG
    CTTCTGAAGC AACTGACCAC CCAACAGCAT GCAATACTCA TTGATCTAGG ACGCACCTTT
    CCAACACACC CGTACTTCTC GGCCCAGCTG GGGGCCGGAC AGCTGTCACT CTACAACATT
    CTAAAGGCCT ACTCCTTGCT GGATCCCGAA GTGGGCTACT GCCAGGGCCT CAGCTTTGTC
    GCTGGCGTCC TTTTGCTTCA CATGACCGAG GAAGACGCCT TTAAGATGCT AAAGTTTCTC
    ATGTATGACT TGGGTCTGAG GAAGCAGTAT CGGCCCGACA TGATAACTCT TCAGATCCAG
    ATGTACCAGC TTTCCCGACT CCTGCACGAC TATCACCGGG ATCTGTACAA TCACTTGGAA
    ACCTATGAGA TCGGGCCCAG CCTGTATGCT GCTCCTTGGT TCCTTACCAT GTTCGCCTCT
    CAGTTCCCGC TGGGATTTGT GGCGAGAGTC TTTGATATGA TTTTCCTTCA GGGATCAGAA
    GTCATATTTA AGGTGGCCCT CAGCTTGCTC GGAAGCCACA AGCCCTTGAT TTTGCAGCAT
    GAAAATTTGG AATCTATTGT AGAATTTATA AAGAACACTC TTCCAAACTT GGGTCTGGTG
    CAGATGGAAA AGACCATTAA CCAGGTGTTT GAAATGGACA TTTCCAAGCA GCTTCAGGCA
    TATGAAGTGG AATATCATGT ACTGCAGGAT GAACTGATTG ATTCATCTCC TCTCAGTGAC
    AACCAGAGGA TAAATAAACT AGAGAAGGCA AATAGTACAT TGCGCAAACA GAACTTTGAC
    CTTTTGGAGG AGCTGCAGGT ATCACATGGG AGAATACAGG TGCTAGAATC CACCGTAGAA
    AACTTACAGA GCAACGAGGT CAAGTTAAAA CGATCCGTCC TTACTCTGGA GCTGGAAAGG
    TCTGCCTTGC TAAATACCAT CGAAGAATTA CGTGCTCAGA TTAAAGATAT GCAAGGGAAC
    AAGCTCTGTA ATGACCAAAA TGAGCCCAAC CAGCTCAACA AGGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49677
    Clone ID Related Accession (Same CDS sequence) XM_004910931.2
    Accession Version XM_004910931.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910931.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCCGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACCGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA GGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAGA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACA CTCCTCTGCC TGAGCCGGAT CACAATCCAG TGCAGTCTGC AGAACCGACT
    GCTCCTCGTC CCGTCTCTGC AACCTCTTCC CCTGTAACTG GGAAAAAACT GAAGGTCGGT
    TCTGTTGGGG AAGCAGGGGC GCCACTTTCA CAAAAGAGCC TTTTCTACCG TTCTACTGCT
    CTTCGAAAGA AACTTCATTC CTCATCTTCT GTGCCAAACT TTCTCAAATT TCTGGCCCCT
    GTAGCGGAAA ATAGCACCGC CGGCTTTAAC AGTAGCAAAA GTGACTTTGA ATGTAAGTCA
    ATACACAGCG AAGCTGGATG TGAGAGCCCA GTAAGGACGC GCAGACATTC TTGGCGGCAA
    CAGATCTTCT TACGTGTAGC CACCCCCCAA AAGGGCTGCG ATTCCCCCAA CAGATATGAT
    GACCACTCAG ATTTGGGGGA CATTGCACCC AGATCACCTC TAGAACCTGT TTCTGAAGAT
    GGTCCAGTCA GGTCGGCAGT TGCAGTAAGG CACCGGACAA CAGAAGAGCT TCGAGAGCTC
    TGGAAAAAGG CAATTCTTCA ACAAATTCTG CTTCTCAGGA TGGAAAAAGA AAACCAAAAA
    CTGCAAGCCT CAGAAAATGA CTTGCAGAAT AAGAGGCTGA AACTTGACTA TGAAGAAATC
    ACTCCATGCC TGAAAGATGT GACTTTAGTT TGGGAGAAGA TGCTCAGCAC ACCCGGGAGA
    TCAAAGATCA AGACTGACAC AGAAAAAATA CATGCAGCTG TTGGACAAGG GGTCCCCCGT
    CAGCACCGTG GGGAGATCTG GAAGTTTTTA TCAGAACAGT ACCAGCTGAG ACATCAGCAT
    ATAAGTAAGA ACCAGTTAAA AGACACCCCG TACAAGGAGC TTCTGAAGCA ACTGACCACC
    CAACAGCATG CAATACTCAT TGATCTAGGA CGCACCTTTC CAACACACCC GTACTTCTCG
    GCCCAGCTGG GGGCCGGACA GCTGTCACTC TACAACATTC TAAAGGCCTA CTCCTTGCTG
    GATCCCGAAG TGGGCTACTG CCAGGGCCTC AGCTTTGTCG CTGGCGTCCT TTTGCTTCAC
    ATGACCGAGG AAGACGCCTT TAAGATGCTA AAGTTTCTCA TGTATGACTT GGGTCTGAGG
    AAGCAGTATC GGCCCGACAT GATAACTCTT CAGATCCAGA TGTACCAGCT TTCCCGACTC
    CTGCACGACT ATCACCGGGA TCTGTACAAT CACTTGGAAA CCTATGAGAT CGGGCCCAGC
    CTGTATGCTG CTCCTTGGTT CCTTACCATG TTCGCCTCTC AGTTCCCGCT GGGATTTGTG
    GCGAGAGTCT TTGATATGAT TTTCCTTCAG GGATCAGAAG TCATATTTAA GGTGGCCCTC
    AGCTTGCTCG GAAGCCACAA GCCCTTGATT TTGCAGCATG AAAATTTGGA ATCTATTGTA
    GAATTTATAA AGAACACTCT TCCAAACTTG GGTCTGGTGC AGATGGAAAA GACCATTAAC
    CAGGTGTTTG AAATGGACAT TTCCAAGCAG CTTCAGGCAT ATGAAGTGGA ATATCATGTA
    CTGCAGGATG AACTGATTGA TTCATCTCCT CTCAGTGACA ACCAGAGGAT AAATAAACTA
    GAGAAGGCAA ATAGTACATT GCGCAAACAG AACTTTGACC TTTTGGAGGA GCTGCAGGTA
    TCACATGGGA GAATACAGGT GCTAGAATCC ACCGTAGAAA ACTTACAGAG CAACGAGGTC
    AAGTTAAAAC GATCCGTCCT TACTCTGGAG CTGGAAAGGT CTGCCTTGCT AAATACCATC
    GAAGAATTAC GTGCTCAGAT TAAAGATATG CAAGGGAACA AGCTCTGTAA TGACCAAAAT
    GAGCCCAACC AGCTCAACAA GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910988.2
    CDS112..3618
    Translation

    Target ORF information:

    RefSeq Version XM_004910931.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910931.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCCGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACCGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA GGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAGA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACA CTCCTCTGCC TGAGCCGGAT CACAATCCAG TGCAGTCTGC AGAACCGACT
    GCTCCTCGTC CCGTCTCTGC AACCTCTTCC CCTGTAACTG GGAAAAAACT GAAGGTCGGT
    TCTGTTGGGG AAGCAGGGGC GCCACTTTCA CAAAAGAGCC TTTTCTACCG TTCTACTGCT
    CTTCGAAAGA AACTTCATTC CTCATCTTCT GTGCCAAACT TTCTCAAATT TCTGGCCCCT
    GTAGCGGAAA ATAGCACCGC CGGCTTTAAC AGTAGCAAAA GTGACTTTGA ATGTAAGTCA
    ATACACAGCG AAGCTGGATG TGAGAGCCCA GTAAGGACGC GCAGACATTC TTGGCGGCAA
    CAGATCTTCT TACGTGTAGC CACCCCCCAA AAGGGCTGCG ATTCCCCCAA CAGATATGAT
    GACCACTCAG ATTTGGGGGA CATTGCACCC AGATCACCTC TAGAACCTGT TTCTGAAGAT
    GGTCCAGTCA GGTCGGCAGT TGCAGTAAGG CACCGGACAA CAGAAGAGCT TCGAGAGCTC
    TGGAAAAAGG CAATTCTTCA ACAAATTCTG CTTCTCAGGA TGGAAAAAGA AAACCAAAAA
    CTGCAAGCCT CAGAAAATGA CTTGCAGAAT AAGAGGCTGA AACTTGACTA TGAAGAAATC
    ACTCCATGCC TGAAAGATGT GACTTTAGTT TGGGAGAAGA TGCTCAGCAC ACCCGGGAGA
    TCAAAGATCA AGACTGACAC AGAAAAAATA CATGCAGCTG TTGGACAAGG GGTCCCCCGT
    CAGCACCGTG GGGAGATCTG GAAGTTTTTA TCAGAACAGT ACCAGCTGAG ACATCAGCAT
    ATAAGTAAGA ACCAGTTAAA AGACACCCCG TACAAGGAGC TTCTGAAGCA ACTGACCACC
    CAACAGCATG CAATACTCAT TGATCTAGGA CGCACCTTTC CAACACACCC GTACTTCTCG
    GCCCAGCTGG GGGCCGGACA GCTGTCACTC TACAACATTC TAAAGGCCTA CTCCTTGCTG
    GATCCCGAAG TGGGCTACTG CCAGGGCCTC AGCTTTGTCG CTGGCGTCCT TTTGCTTCAC
    ATGACCGAGG AAGACGCCTT TAAGATGCTA AAGTTTCTCA TGTATGACTT GGGTCTGAGG
    AAGCAGTATC GGCCCGACAT GATAACTCTT CAGATCCAGA TGTACCAGCT TTCCCGACTC
    CTGCACGACT ATCACCGGGA TCTGTACAAT CACTTGGAAA CCTATGAGAT CGGGCCCAGC
    CTGTATGCTG CTCCTTGGTT CCTTACCATG TTCGCCTCTC AGTTCCCGCT GGGATTTGTG
    GCGAGAGTCT TTGATATGAT TTTCCTTCAG GGATCAGAAG TCATATTTAA GGTGGCCCTC
    AGCTTGCTCG GAAGCCACAA GCCCTTGATT TTGCAGCATG AAAATTTGGA ATCTATTGTA
    GAATTTATAA AGAACACTCT TCCAAACTTG GGTCTGGTGC AGATGGAAAA GACCATTAAC
    CAGGTGTTTG AAATGGACAT TTCCAAGCAG CTTCAGGCAT ATGAAGTGGA ATATCATGTA
    CTGCAGGATG AACTGATTGA TTCATCTCCT CTCAGTGACA ACCAGAGGAT AAATAAACTA
    GAGAAGGCAA ATAGTACATT GCGCAAACAG AACTTTGACC TTTTGGAGGA GCTGCAGGTA
    TCACATGGGA GAATACAGGT GCTAGAATCC ACCGTAGAAA ACTTACAGAG CAACGAGGTC
    AAGTTAAAAC GATCCGTCCT TACTCTGGAG CTGGAAAGGT CTGCCTTGCT AAATACCATC
    GAAGAATTAC GTGCTCAGAT TAAAGATATG CAAGGGAACA AGCTCTGTAA TGACCAAAAT
    GAGCCCAACC AGCTCAACAA GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49678
    Clone ID Related Accession (Same CDS sequence) XM_004910932.4
    Accession Version XM_004910932.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910932.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCACCTC TGTTTAGCTG GGTGGCCAAG GTGCCGGAAA TAATTGGCTC TATCCGGCAT 
    GCAGGAAAGT TGGCACGGCA GGAAGAGGTT CATTCCCAGC TTTGTGATTT GGACGATTCA
    TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA
    CCCCCTGCTT TGATAGACGA GTGCATTGAC AAGTTCAACC ATGTTAGCTG TACTAAAAAG
    AAGGAAGGGG ACTCTCCGAC TCCACAGGGG GACTCTCCAC CTCCACAACC GGACTCCGGC
    ACTGGGATAT CTTTCGGCGA TAAACTGCGC TCAGTCTTCC AATCGGCATT TCACGATGAT
    GACAAGAATA AGCCCATCCG CAAATCCTAC TCCCAGCCAG GCCTGCGGAC TAATACCTCT
    TTTACAAATG AATGGAAAGA TGGACGCAGT AGCCTTCCTA CTTCATTTGA GGAAAGCGAT
    ATAAGTTTGC ATGTACAAAG GTTATGTGGG GAGGAACAGG ACAGTGTGCA GCCAACAGAC
    ATGGAGGAAA ACCAGACTAT GCTATTCACG ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC
    CCAGACACAA AGAGGATTGT GATTGAAAAG AGCTTTAAGG AGATATCATT TTGTTCTCAG
    GGAATCCAGC ATGCTGACCA CTTTGGTTTT ATCTGCAGGG AGCCATTGCT GAATGGAGGG
    TGCCATTTTG TGTGTTATGT ATTTCAGTGT GCAAATGGAG CACTGGTGGA TGAGATCATG
    CTGACTCTGA AGCAGGCCTT CAGTGTAGCT GCAGTGCAGC AGAACTCCAA GACTCTTCCG
    CAGCTCTGCG ATACTTGTCC CATGCAGTGC ATGCATAAAC TCTGTGAGAG AATAGAAGGT
    CTTCCTCCTA CCAAAATAAA ACTTGAACTG CAGAAGCACA TCACAACACT AAGCAATCAG
    GAGCAGGCCT CCATGTTTGA AGAAATACAG AAAATCCGGC CACGGTCAGA GCAGCAAGAA
    AATGAACTAG TCATCTCTCG CCTTCGATGT CTGTATGAGG AGAAACAGAG AGACCATATC
    CATAACGCTG AAACAAAGCA GAATTCGCAA GCAGCCGAAA GCACAGCCAA TGGGCTGCAG
    ACCAGTGCAT CTCGATTCAA ACTTGAGTCT CTGACCAGCA AAGCAAAGAG ATCTTTGACG
    GAATCTTTAG AAAACATTTT GTCAAGGGGC AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT
    GCCAGTGGGG ATGATGACAG CTCACTATGC ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG
    TCATTATGTG AGACAGAACC GGCTGCTCCG CTTGCAATCC CCTTAAAGAC TCACGGCTCC
    CTTGGAGATT TATCGAAGAG CTCAGATAGC TATTCCTGTG AGCATCCGGA GGAATTAGTT
    CAGCAGGGAT TCCGCCGGCG AGCTAACACA TTAAGCCATT TCCCTGTCAC AGCGCAGGAA
    TTTCCCGAGC CGGTTCAGAT AACTCCATCT GTTTCACAGC GGAAGCTAAT AAGGCACCAC
    TCTGTGAGCA CCGAGACGCC GCACAAAAGA AACACTCCTC TGCCTGAGCC GGATCACAAT
    CCAGTGCAGT CTGCAGAACC GACTGCTCCT CGTCCCGTCT CTGCAACCTC TTCCCCTGTA
    ACTGGGAAAA AACTGAAGGT CGGTTCTGTT GGGGAAGCAG GGGCGCCACT TTCACAAAAG
    AGCCTTTTCT ACCGTTCTAC TGCTCTTCGA AAGAAACTTC ATTCCTCATC TTCTGTGCCA
    AACTTTCTCA AATTTCTGGC CCCTGTAGCG GAAAATAGCA CCGCCGGCTT TAACAGTAGC
    AAAAGTGACT TTGAATGTAA GTCAATACAC AGCGAAGCTG GATGTGAGAG CCCAGTAAGG
    ACGCGCAGAC ATTCTTGGCG GCAACAGATC TTCTTACGTG TAGCCACCCC CCAAAAGGGC
    TGCGATTCCC CCAACAGATA TGATGACCAC TCAGATTTGG GGGACATTGC ACCCAGATCA
    CCTCTAGAAC CTGTTTCTGA AGATGGTCCA GTCAGGTCGG CAGTTGCAGT AAGGCACCGG
    ACAACAGAAG AGCTTCGAGA GCTCTGGAAA AAGGCAATTC TTCAACAAAT TCTGCTTCTC
    AGGATGGAAA AAGAAAACCA AAAACTGCAA GCCTCAGAAA ATGACTTGCA GAATAAGAGG
    CTGAAACTTG ACTATGAAGA AATCACTCCA TGCCTGAAAG ATGTGACTTT AGTTTGGGAG
    AAGATGCTCA GCACACCCGG GAGATCAAAG ATCAAGACTG ACACAGAAAA AATACATGCA
    GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC CGTGGGGAGA TCTGGAAGTT TTTATCAGAA
    CAGTACCAGC TGAGACATCA GCATATAAGT AAGAACCAGT TAAAAGACAC CCCGTACAAG
    GAGCTTCTGA AGCAACTGAC CACCCAACAG CATGCAATAC TCATTGATCT AGGACGCACC
    TTTCCAACAC ACCCGTACTT CTCGGCCCAG CTGGGGGCCG GACAGCTGTC ACTCTACAAC
    ATTCTAAAGG CCTACTCCTT GCTGGATCCC GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT
    GTCGCTGGCG TCCTTTTGCT TCACATGACC GAGGAAGACG CCTTTAAGAT GCTAAAGTTT
    CTCATGTATG ACTTGGGTCT GAGGAAGCAG TATCGGCCCG ACATGATAAC TCTTCAGATC
    CAGATGTACC AGCTTTCCCG ACTCCTGCAC GACTATCACC GGGATCTGTA CAATCACTTG
    GAAACCTATG AGATCGGGCC CAGCCTGTAT GCTGCTCCTT GGTTCCTTAC CATGTTCGCC
    TCTCAGTTCC CGCTGGGATT TGTGGCGAGA GTCTTTGATA TGATTTTCCT TCAGGGATCA
    GAAGTCATAT TTAAGGTGGC CCTCAGCTTG CTCGGAAGCC ACAAGCCCTT GATTTTGCAG
    CATGAAAATT TGGAATCTAT TGTAGAATTT ATAAAGAACA CTCTTCCAAA CTTGGGTCTG
    GTGCAGATGG AAAAGACCAT TAACCAGGTG TTTGAAATGG ACATTTCCAA GCAGCTTCAG
    GCATATGAAG TGGAATATCA TGTACTGCAG GATGAACTGA TTGATTCATC TCCTCTCAGT
    GACAACCAGA GGATAAATAA ACTAGAGAAG GCAAATAGTA CATTGCGCAA ACAGAACTTT
    GACCTTTTGG AGGAGCTGCA GGTATCACAT GGGAGAATAC AGGTGCTAGA ATCCACCGTA
    GAAAACTTAC AGAGCAACGA GGTCAAGTTA AAACGATCCG TCCTTACTCT GGAGCTGGAA
    AGGTCTGCCT TGCTAAATAC CATCGAAGAA TTACGTGCTC AGATTAAAGA TATGCAAGGG
    AACAAGCTCT GTAATGACCA AAATGAGCCC AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910989.2
    CDS159..3629
    Translation

    Target ORF information:

    RefSeq Version XM_004910932.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910932.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCACCTC TGTTTAGCTG GGTGGCCAAG GTGCCGGAAA TAATTGGCTC TATCCGGCAT 
    GCAGGAAAGT TGGCACGGCA GGAAGAGGTT CATTCCCAGC TTTGTGATTT GGACGATTCA
    TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA
    CCCCCTGCTT TGATAGACGA GTGCATTGAC AAGTTCAACC ATGTTAGCTG TACTAAAAAG
    AAGGAAGGGG ACTCTCCGAC TCCACAGGGG GACTCTCCAC CTCCACAACC GGACTCCGGC
    ACTGGGATAT CTTTCGGCGA TAAACTGCGC TCAGTCTTCC AATCGGCATT TCACGATGAT
    GACAAGAATA AGCCCATCCG CAAATCCTAC TCCCAGCCAG GCCTGCGGAC TAATACCTCT
    TTTACAAATG AATGGAAAGA TGGACGCAGT AGCCTTCCTA CTTCATTTGA GGAAAGCGAT
    ATAAGTTTGC ATGTACAAAG GTTATGTGGG GAGGAACAGG ACAGTGTGCA GCCAACAGAC
    ATGGAGGAAA ACCAGACTAT GCTATTCACG ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC
    CCAGACACAA AGAGGATTGT GATTGAAAAG AGCTTTAAGG AGATATCATT TTGTTCTCAG
    GGAATCCAGC ATGCTGACCA CTTTGGTTTT ATCTGCAGGG AGCCATTGCT GAATGGAGGG
    TGCCATTTTG TGTGTTATGT ATTTCAGTGT GCAAATGGAG CACTGGTGGA TGAGATCATG
    CTGACTCTGA AGCAGGCCTT CAGTGTAGCT GCAGTGCAGC AGAACTCCAA GACTCTTCCG
    CAGCTCTGCG ATACTTGTCC CATGCAGTGC ATGCATAAAC TCTGTGAGAG AATAGAAGGT
    CTTCCTCCTA CCAAAATAAA ACTTGAACTG CAGAAGCACA TCACAACACT AAGCAATCAG
    GAGCAGGCCT CCATGTTTGA AGAAATACAG AAAATCCGGC CACGGTCAGA GCAGCAAGAA
    AATGAACTAG TCATCTCTCG CCTTCGATGT CTGTATGAGG AGAAACAGAG AGACCATATC
    CATAACGCTG AAACAAAGCA GAATTCGCAA GCAGCCGAAA GCACAGCCAA TGGGCTGCAG
    ACCAGTGCAT CTCGATTCAA ACTTGAGTCT CTGACCAGCA AAGCAAAGAG ATCTTTGACG
    GAATCTTTAG AAAACATTTT GTCAAGGGGC AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT
    GCCAGTGGGG ATGATGACAG CTCACTATGC ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG
    TCATTATGTG AGACAGAACC GGCTGCTCCG CTTGCAATCC CCTTAAAGAC TCACGGCTCC
    CTTGGAGATT TATCGAAGAG CTCAGATAGC TATTCCTGTG AGCATCCGGA GGAATTAGTT
    CAGCAGGGAT TCCGCCGGCG AGCTAACACA TTAAGCCATT TCCCTGTCAC AGCGCAGGAA
    TTTCCCGAGC CGGTTCAGAT AACTCCATCT GTTTCACAGC GGAAGCTAAT AAGGCACCAC
    TCTGTGAGCA CCGAGACGCC GCACAAAAGA AACACTCCTC TGCCTGAGCC GGATCACAAT
    CCAGTGCAGT CTGCAGAACC GACTGCTCCT CGTCCCGTCT CTGCAACCTC TTCCCCTGTA
    ACTGGGAAAA AACTGAAGGT CGGTTCTGTT GGGGAAGCAG GGGCGCCACT TTCACAAAAG
    AGCCTTTTCT ACCGTTCTAC TGCTCTTCGA AAGAAACTTC ATTCCTCATC TTCTGTGCCA
    AACTTTCTCA AATTTCTGGC CCCTGTAGCG GAAAATAGCA CCGCCGGCTT TAACAGTAGC
    AAAAGTGACT TTGAATGTAA GTCAATACAC AGCGAAGCTG GATGTGAGAG CCCAGTAAGG
    ACGCGCAGAC ATTCTTGGCG GCAACAGATC TTCTTACGTG TAGCCACCCC CCAAAAGGGC
    TGCGATTCCC CCAACAGATA TGATGACCAC TCAGATTTGG GGGACATTGC ACCCAGATCA
    CCTCTAGAAC CTGTTTCTGA AGATGGTCCA GTCAGGTCGG CAGTTGCAGT AAGGCACCGG
    ACAACAGAAG AGCTTCGAGA GCTCTGGAAA AAGGCAATTC TTCAACAAAT TCTGCTTCTC
    AGGATGGAAA AAGAAAACCA AAAACTGCAA GCCTCAGAAA ATGACTTGCA GAATAAGAGG
    CTGAAACTTG ACTATGAAGA AATCACTCCA TGCCTGAAAG ATGTGACTTT AGTTTGGGAG
    AAGATGCTCA GCACACCCGG GAGATCAAAG ATCAAGACTG ACACAGAAAA AATACATGCA
    GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC CGTGGGGAGA TCTGGAAGTT TTTATCAGAA
    CAGTACCAGC TGAGACATCA GCATATAAGT AAGAACCAGT TAAAAGACAC CCCGTACAAG
    GAGCTTCTGA AGCAACTGAC CACCCAACAG CATGCAATAC TCATTGATCT AGGACGCACC
    TTTCCAACAC ACCCGTACTT CTCGGCCCAG CTGGGGGCCG GACAGCTGTC ACTCTACAAC
    ATTCTAAAGG CCTACTCCTT GCTGGATCCC GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT
    GTCGCTGGCG TCCTTTTGCT TCACATGACC GAGGAAGACG CCTTTAAGAT GCTAAAGTTT
    CTCATGTATG ACTTGGGTCT GAGGAAGCAG TATCGGCCCG ACATGATAAC TCTTCAGATC
    CAGATGTACC AGCTTTCCCG ACTCCTGCAC GACTATCACC GGGATCTGTA CAATCACTTG
    GAAACCTATG AGATCGGGCC CAGCCTGTAT GCTGCTCCTT GGTTCCTTAC CATGTTCGCC
    TCTCAGTTCC CGCTGGGATT TGTGGCGAGA GTCTTTGATA TGATTTTCCT TCAGGGATCA
    GAAGTCATAT TTAAGGTGGC CCTCAGCTTG CTCGGAAGCC ACAAGCCCTT GATTTTGCAG
    CATGAAAATT TGGAATCTAT TGTAGAATTT ATAAAGAACA CTCTTCCAAA CTTGGGTCTG
    GTGCAGATGG AAAAGACCAT TAACCAGGTG TTTGAAATGG ACATTTCCAA GCAGCTTCAG
    GCATATGAAG TGGAATATCA TGTACTGCAG GATGAACTGA TTGATTCATC TCCTCTCAGT
    GACAACCAGA GGATAAATAA ACTAGAGAAG GCAAATAGTA CATTGCGCAA ACAGAACTTT
    GACCTTTTGG AGGAGCTGCA GGTATCACAT GGGAGAATAC AGGTGCTAGA ATCCACCGTA
    GAAAACTTAC AGAGCAACGA GGTCAAGTTA AAACGATCCG TCCTTACTCT GGAGCTGGAA
    AGGTCTGCCT TGCTAAATAC CATCGAAGAA TTACGTGCTC AGATTAAAGA TATGCAAGGG
    AACAAGCTCT GTAATGACCA AAATGAGCCC AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa49679
    Clone ID Related Accession (Same CDS sequence) XM_004910933.3
    Accession Version XM_004910933.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910933.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCCGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACCGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA GGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAGA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAATG ACTTTGAATG TAAGTCAATA CACAGCGAAG CTGGATGTGA GAGCCCAGTA
    AGGACGCGCA GACATTCTTG GCGGCAACAG ATCTTCTTAC GTGTAGCCAC CCCCCAAAAG
    GGCTGCGATT CCCCCAACAG ATATGATGAC CACTCAGATT TGGGGGACAT TGCACCCAGA
    TCACCTCTAG AACCTGTTTC TGAAGATGGT CCAGTCAGGT CGGCAGTTGC AGTAAGGCAC
    CGGACAACAG AAGAGCTTCG AGAGCTCTGG AAAAAGGCAA TTCTTCAACA AATTCTGCTT
    CTCAGGATGG AAAAAGAAAA CCAAAAACTG CAAGCCTCAG AAAATGACTT GCAGAATAAG
    AGGCTGAAAC TTGACTATGA AGAAATCACT CCATGCCTGA AAGATGTGAC TTTAGTTTGG
    GAGAAGATGC TCAGCACACC CGGGAGATCA AAGATCAAGA CTGACACAGA AAAAATACAT
    GCAGCTGTTG GACAAGGGGT CCCCCGTCAG CACCGTGGGG AGATCTGGAA GTTTTTATCA
    GAACAGTACC AGCTGAGACA TCAGCATATA AGTAAGAACC AGTTAAAAGA CACCCCGTAC
    AAGGAGCTTC TGAAGCAACT GACCACCCAA CAGCATGCAA TACTCATTGA TCTAGGACGC
    ACCTTTCCAA CACACCCGTA CTTCTCGGCC CAGCTGGGGG CCGGACAGCT GTCACTCTAC
    AACATTCTAA AGGCCTACTC CTTGCTGGAT CCCGAAGTGG GCTACTGCCA GGGCCTCAGC
    TTTGTCGCTG GCGTCCTTTT GCTTCACATG ACCGAGGAAG ACGCCTTTAA GATGCTAAAG
    TTTCTCATGT ATGACTTGGG TCTGAGGAAG CAGTATCGGC CCGACATGAT AACTCTTCAG
    ATCCAGATGT ACCAGCTTTC CCGACTCCTG CACGACTATC ACCGGGATCT GTACAATCAC
    TTGGAAACCT ATGAGATCGG GCCCAGCCTG TATGCTGCTC CTTGGTTCCT TACCATGTTC
    GCCTCTCAGT TCCCGCTGGG ATTTGTGGCG AGAGTCTTTG ATATGATTTT CCTTCAGGGA
    TCAGAAGTCA TATTTAAGGT GGCCCTCAGC TTGCTCGGAA GCCACAAGCC CTTGATTTTG
    CAGCATGAAA ATTTGGAATC TATTGTAGAA TTTATAAAGA ACACTCTTCC AAACTTGGGT
    CTGGTGCAGA TGGAAAAGAC CATTAACCAG GTGTTTGAAA TGGACATTTC CAAGCAGCTT
    CAGGCATATG AAGTGGAATA TCATGTACTG CAGGATGAAC TGATTGATTC ATCTCCTCTC
    AGTGACAACC AGAGGATAAA TAAACTAGAG AAGGCAAATA GTACATTGCG CAAACAGAAC
    TTTGACCTTT TGGAGGAGCT GCAGGTATCA CATGGGAGAA TACAGGTGCT AGAATCCACC
    GTAGAAAACT TACAGAGCAA CGAGGTCAAG TTAAAACGAT CCGTCCTTAC TCTGGAGCTG
    GAAAGGTCTG CCTTGCTAAA TACCATCGAA GAATTACGTG CTCAGATTAA AGATATGCAA
    GGGAACAAGC TCTGTAATGA CCAAAATGAG CCCAACCAGC TCAACAAGGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910990.2
    CDS108..3341
    Translation

    Target ORF information:

    RefSeq Version XM_004910933.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910933.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCCGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACCGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA GGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAGA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAATG ACTTTGAATG TAAGTCAATA CACAGCGAAG CTGGATGTGA GAGCCCAGTA
    AGGACGCGCA GACATTCTTG GCGGCAACAG ATCTTCTTAC GTGTAGCCAC CCCCCAAAAG
    GGCTGCGATT CCCCCAACAG ATATGATGAC CACTCAGATT TGGGGGACAT TGCACCCAGA
    TCACCTCTAG AACCTGTTTC TGAAGATGGT CCAGTCAGGT CGGCAGTTGC AGTAAGGCAC
    CGGACAACAG AAGAGCTTCG AGAGCTCTGG AAAAAGGCAA TTCTTCAACA AATTCTGCTT
    CTCAGGATGG AAAAAGAAAA CCAAAAACTG CAAGCCTCAG AAAATGACTT GCAGAATAAG
    AGGCTGAAAC TTGACTATGA AGAAATCACT CCATGCCTGA AAGATGTGAC TTTAGTTTGG
    GAGAAGATGC TCAGCACACC CGGGAGATCA AAGATCAAGA CTGACACAGA AAAAATACAT
    GCAGCTGTTG GACAAGGGGT CCCCCGTCAG CACCGTGGGG AGATCTGGAA GTTTTTATCA
    GAACAGTACC AGCTGAGACA TCAGCATATA AGTAAGAACC AGTTAAAAGA CACCCCGTAC
    AAGGAGCTTC TGAAGCAACT GACCACCCAA CAGCATGCAA TACTCATTGA TCTAGGACGC
    ACCTTTCCAA CACACCCGTA CTTCTCGGCC CAGCTGGGGG CCGGACAGCT GTCACTCTAC
    AACATTCTAA AGGCCTACTC CTTGCTGGAT CCCGAAGTGG GCTACTGCCA GGGCCTCAGC
    TTTGTCGCTG GCGTCCTTTT GCTTCACATG ACCGAGGAAG ACGCCTTTAA GATGCTAAAG
    TTTCTCATGT ATGACTTGGG TCTGAGGAAG CAGTATCGGC CCGACATGAT AACTCTTCAG
    ATCCAGATGT ACCAGCTTTC CCGACTCCTG CACGACTATC ACCGGGATCT GTACAATCAC
    TTGGAAACCT ATGAGATCGG GCCCAGCCTG TATGCTGCTC CTTGGTTCCT TACCATGTTC
    GCCTCTCAGT TCCCGCTGGG ATTTGTGGCG AGAGTCTTTG ATATGATTTT CCTTCAGGGA
    TCAGAAGTCA TATTTAAGGT GGCCCTCAGC TTGCTCGGAA GCCACAAGCC CTTGATTTTG
    CAGCATGAAA ATTTGGAATC TATTGTAGAA TTTATAAAGA ACACTCTTCC AAACTTGGGT
    CTGGTGCAGA TGGAAAAGAC CATTAACCAG GTGTTTGAAA TGGACATTTC CAAGCAGCTT
    CAGGCATATG AAGTGGAATA TCATGTACTG CAGGATGAAC TGATTGATTC ATCTCCTCTC
    AGTGACAACC AGAGGATAAA TAAACTAGAG AAGGCAAATA GTACATTGCG CAAACAGAAC
    TTTGACCTTT TGGAGGAGCT GCAGGTATCA CATGGGAGAA TACAGGTGCT AGAATCCACC
    GTAGAAAACT TACAGAGCAA CGAGGTCAAG TTAAAACGAT CCGTCCTTAC TCTGGAGCTG
    GAAAGGTCTG CCTTGCTAAA TACCATCGAA GAATTACGTG CTCAGATTAA AGATATGCAA
    GGGAACAAGC TCTGTAATGA CCAAAATGAG CCCAACCAGC TCAACAAGGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04183
    Clone ID Related Accession (Same CDS sequence) XM_004910931.1
    Accession Version XM_004910931.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3507bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACA CTCCTCTGCC TGAGCCGGAT CACAATCCAG TGCAGTCTGC AGAACCGACT
    GCTCCTCGTC CCGTCTCTGC AACCTCTTCC CCTGTAACTG GGAAAAAACT GAAGGTCGGT
    TCTGTTGGGG AAGCAGGGGC GCCACTTTCA CAAAAGAGCC TTTTCTACCG TTCTACTGCT
    CTTCGAAAGA AACTTCATTC CTCATCTTCT GTGCCAAACT TTCTCAAATT TCTGGCCCCT
    GTAGCGGAAA ATAGCACCGC CGGCTTTAAC AGTAGCAAAA GTGACTTTGA ATGTAAGTCA
    ATACACAGCG AAGCTGGATG TGAGAGCCCA GTAAGGACGC GCAGACATTC TTGGCGGCAA
    CAGATCTTCT TACGTGTAGC CACCCCCCAA AAGGGCTGCG ATTCCCCCAA CAGATATGAT
    GACCACTCAG ATTTGGGGGA CATTGCACCC AGATCACCTC TAGAACCTGT TTCTGAAGAT
    GGTCCAGTCA GGTCGGCAGT TGCAGTAAGG CACCGGACAA CAGAAGAGCT TCGAGAGCTC
    TGGAAAAAGG CAATTCTTCA ACAAATTCTG CTTCTCAGGA TGGAAAAAGA AAACCAAAAA
    CTGCAAGCCT CAGAAAATGA CTTGCAGAAT AAGAGGCTGA AACTTGACTA TGAAGAAATC
    ACTCCATGCC TGAAAGATGT GACTTTAGTT TGGGAGAAGA TGCTCAGCAC ACCCGGGAGA
    TCAAAGATCA AGACTGACAC AGAAAAAATA CATGCAGCTG TTGGACAAGG GGTCCCCCGT
    CAGCACCGTG GGGAGATCTG GAAGTTTTTA TCAGAACAGT ACCAGCTGAG ACATCAGCAT
    ATAAGTAAGA ACCAGTTAAA AGACACCCCG TACAAGGAGC TTCTGAAGCA ACTGACCACC
    CAACAGCATG CAATACTCAT TGATCTAGGA CGCACCTTTC CAACACACCC GTACTTCTCG
    GCCCAGCTGG GGGCCGGACA GCTGTCACTC TACAACATTC TAAAGGCCTA CTCCTTGCTG
    GATCCCGAAG TGGGCTACTG CCAGGGCCTC AGCTTTGTCG CTGGCGTCCT TTTGCTTCAC
    ATGACCGAGG AAGACGCCTT TAAGATGCTA AAGTTTCTCA TGTATGACTT GGGTCTGAGG
    AAGCAGTATC GGCCCCACAT GATAACTCTT CAGATCCAGA TGTACCAGCT TTCCCGACTC
    CTGCACGACT ATCACCGGGA TCTGTACAAT CACTTGGAAA CCTATGAGAT CGGGCCCAGC
    CTGTATGCTG CTCCTTGGTT CCTTACCATG TTCGCCTCTC AGTTCCCGCT GGGATTTGTG
    GCGAGAGTCT TTGATATGAT TTTCCTTCAG GGATCAGAAG TCATATTTAA GGTGGCCCTC
    AGCTTGCTCG GAAGCCACAA GCCCTTGATT TTGCAGCATG AAAATTTGGA ATCTATTGTA
    GAATTTATAA AGAACACTCT TCCAAACTTG GGTCTGGTGC AGATGGAAAA GACCATTAAC
    CAGGTGTTTG AAATGGACAT TTCCAAGCAG CTTCAGGCAT ATGAAGTGGA ATATCATGTA
    CTGCAGGATG AACTGATTGA TTCATCTCCT CTCAGTGACA ACCAGAGGAT AAATAAACTA
    GAGAAGGCAA ATAGTACATT GCGCAAACAG AACTTTGACC TTTTGGAGGA GCTGCAGGTA
    TCACATGGGA GAATACAGGT GCTAGAATCC ACCGTAGAAA ACTTACAGAG CAACGAGGTC
    AAGTTAAAAC GATCCGTCCT TACTCTGGAG CTGGAAAGGT CTGCCTTGCT AAATACCATC
    GAAGAATTAC GTGCTCAGAT TAAAGATATG CAAGGGAACA AGCTCTGTAA TGACCAAAAT
    GAGCCCAACC AGCTCAACAA GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910988.1
    CDS10..3516
    Translation

    Target ORF information:

    RefSeq Version XM_004910931.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910931.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACA CTCCTCTGCC TGAGCCGGAT CACAATCCAG TGCAGTCTGC AGAACCGACT
    GCTCCTCGTC CCGTCTCTGC AACCTCTTCC CCTGTAACTG GGAAAAAACT GAAGGTCGGT
    TCTGTTGGGG AAGCAGGGGC GCCACTTTCA CAAAAGAGCC TTTTCTACCG TTCTACTGCT
    CTTCGAAAGA AACTTCATTC CTCATCTTCT GTGCCAAACT TTCTCAAATT TCTGGCCCCT
    GTAGCGGAAA ATAGCACCGC CGGCTTTAAC AGTAGCAAAA GTGACTTTGA ATGTAAGTCA
    ATACACAGCG AAGCTGGATG TGAGAGCCCA GTAAGGACGC GCAGACATTC TTGGCGGCAA
    CAGATCTTCT TACGTGTAGC CACCCCCCAA AAGGGCTGCG ATTCCCCCAA CAGATATGAT
    GACCACTCAG ATTTGGGGGA CATTGCACCC AGATCACCTC TAGAACCTGT TTCTGAAGAT
    GGTCCAGTCA GGTCGGCAGT TGCAGTAAGG CACCGGACAA CAGAAGAGCT TCGAGAGCTC
    TGGAAAAAGG CAATTCTTCA ACAAATTCTG CTTCTCAGGA TGGAAAAAGA AAACCAAAAA
    CTGCAAGCCT CAGAAAATGA CTTGCAGAAT AAGAGGCTGA AACTTGACTA TGAAGAAATC
    ACTCCATGCC TGAAAGATGT GACTTTAGTT TGGGAGAAGA TGCTCAGCAC ACCCGGGAGA
    TCAAAGATCA AGACTGACAC AGAAAAAATA CATGCAGCTG TTGGACAAGG GGTCCCCCGT
    CAGCACCGTG GGGAGATCTG GAAGTTTTTA TCAGAACAGT ACCAGCTGAG ACATCAGCAT
    ATAAGTAAGA ACCAGTTAAA AGACACCCCG TACAAGGAGC TTCTGAAGCA ACTGACCACC
    CAACAGCATG CAATACTCAT TGATCTAGGA CGCACCTTTC CAACACACCC GTACTTCTCG
    GCCCAGCTGG GGGCCGGACA GCTGTCACTC TACAACATTC TAAAGGCCTA CTCCTTGCTG
    GATCCCGAAG TGGGCTACTG CCAGGGCCTC AGCTTTGTCG CTGGCGTCCT TTTGCTTCAC
    ATGACCGAGG AAGACGCCTT TAAGATGCTA AAGTTTCTCA TGTATGACTT GGGTCTGAGG
    AAGCAGTATC GGCCCCACAT GATAACTCTT CAGATCCAGA TGTACCAGCT TTCCCGACTC
    CTGCACGACT ATCACCGGGA TCTGTACAAT CACTTGGAAA CCTATGAGAT CGGGCCCAGC
    CTGTATGCTG CTCCTTGGTT CCTTACCATG TTCGCCTCTC AGTTCCCGCT GGGATTTGTG
    GCGAGAGTCT TTGATATGAT TTTCCTTCAG GGATCAGAAG TCATATTTAA GGTGGCCCTC
    AGCTTGCTCG GAAGCCACAA GCCCTTGATT TTGCAGCATG AAAATTTGGA ATCTATTGTA
    GAATTTATAA AGAACACTCT TCCAAACTTG GGTCTGGTGC AGATGGAAAA GACCATTAAC
    CAGGTGTTTG AAATGGACAT TTCCAAGCAG CTTCAGGCAT ATGAAGTGGA ATATCATGTA
    CTGCAGGATG AACTGATTGA TTCATCTCCT CTCAGTGACA ACCAGAGGAT AAATAAACTA
    GAGAAGGCAA ATAGTACATT GCGCAAACAG AACTTTGACC TTTTGGAGGA GCTGCAGGTA
    TCACATGGGA GAATACAGGT GCTAGAATCC ACCGTAGAAA ACTTACAGAG CAACGAGGTC
    AAGTTAAAAC GATCCGTCCT TACTCTGGAG CTGGAAAGGT CTGCCTTGCT AAATACCATC
    GAAGAATTAC GTGCTCAGAT TAAAGATATG CAAGGGAACA AGCTCTGTAA TGACCAAAAT
    GAGCCCAACC AGCTCAACAA GGAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04184
    Clone ID Related Accession (Same CDS sequence) XM_004910932.3
    Accession Version XM_004910932.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3471bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910932.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCACCTC TGTTTAGCTG GGTGGCCAAG GTGCCGGAAA TAATTGGCTC TATCCGGCAT 
    GCAGGAAAGT TGGCACGGCA GGAAGAGGTT CATTCCCAGC TTTGTGATTT GGACGATTCA
    TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA
    CCCCCTGCTT TGATAGACGA GTGCATTGAC AAGTTCAACC ATGTTAGCTG TACTAAAAAG
    AAGGAAGGGG ACTCTCTGAC TCCACAGGGG GACTCTCCAC CTCCACAACA GGACTCCGGC
    ACTGGGATAT CTTTCGGCGA TAAACTGCGC TCAGTCTTCC AATCGGCATT TCACGATGAT
    GACAAGAATA AGCCCATCCG CAAATCCTAC TCCCAGCCAG GCCTGCGGAC TAATACCTCT
    TTTACAAATG AATGGAAAGA TGGACGCAGT AGCCTTCCTA CTTCATTTGA GGAAAGCGAT
    ATAAGTTTGC ATGTACAAAG GTTATGTGGG GAGGAACAGG ACAGTGTGCA GCCAACAGAC
    ATGGAGGAAA ACCAGACTAT GCTATTCACG ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC
    CCAGACACAA AGAGGATTGT GATTGAAAAG AGCTTTAAGG AGATATCATT TTGTTCTCAG
    GGAATCCAGC ATGCTGACCA CTTTGGTTTT ATCTGCAGGG AGCCATTGCT GAATGGAGGG
    TGCCATTTTG TGTGTTATGT ATTTCAGTGT GCAAATGGAG CACTGGTGGA TGAGATCATG
    CTGACTCTGA AGCAAGCCTT CAGTGTAGCT GCAGTGCAGC AGAACTCCAA GACTCTTCCG
    CAGCTCTGCG ATACTTGTCC CATGCAGTGC ATGCATAAAC TCTGTGAGAG AATAGAAGGT
    CTTCCTCCTA CCAAAATAAA ACTTGAACTG CAAAAGCACA TCACAACACT AAGCAATCAG
    GAGCAGGCCT CCATGTTTGA AGAAATACAG AAAATCCGGC CACGGTCAGA GCAGCAAGAA
    AATGAACTAG TCATCTCTCG CCTTCGATGT CTGTATGAGG AGAAACAGAG AGACCATATC
    CATAACGCTG AAACAAAGCA GAATTCGCAA GCAGCCGAAA GCACAGCCAA TGGGCTGCAG
    ACCAGTGCAT CTCGATTCAA ACTTGAGTCT CTGACCAGCA AAGCAAAGAG ATCTTTGACG
    GAATCTTTAG AAAACATTTT GTCAAGGGGC AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT
    GCCAGTGGGG ATGATGACAG CTCACTATGC ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG
    TCATTATGTG AGACAGAACC GGCTGCTCCG CTTGCAATCC CCTTAAAGAC TCACGGCTCC
    CTTGGAGATT TATCGAAGAG CTCAGATAGC TATTCCTGTG AGCATCCGGA GGAATTAGTT
    CAGCAGGGAT TCCGCCGGCG AGCTAACACA TTAAGCCATT TCCCTGTCAC AGCGCAGGAA
    TTTCCCGAGC CGGTTCAGAT AACTCCATCT GTTTCACAGC GGAAGCTAAT AAGGCACCAC
    TCTGTGAGCA CCGAGACGCC GCACAAAAGA AACACTCCTC TGCCTGAGCC GGATCACAAT
    CCAGTGCAGT CTGCAGAACC GACTGCTCCT CGTCCCGTCT CTGCAACCTC TTCCCCTGTA
    ACTGGGAAAA AACTGAAGGT CGGTTCTGTT GGGGAAGCAG GGGCGCCACT TTCACAAAAG
    AGCCTTTTCT ACCGTTCTAC TGCTCTTCGA AAGAAACTTC ATTCCTCATC TTCTGTGCCA
    AACTTTCTCA AATTTCTGGC CCCTGTAGCG GAAAATAGCA CCGCCGGCTT TAACAGTAGC
    AAAAGTGACT TTGAATGTAA GTCAATACAC AGCGAAGCTG GATGTGAGAG CCCAGTAAGG
    ACGCGCAGAC ATTCTTGGCG GCAACAGATC TTCTTACGTG TAGCCACCCC CCAAAAGGGC
    TGCGATTCCC CCAACAGATA TGATGACCAC TCAGATTTGG GGGACATTGC ACCCAGATCA
    CCTCTAGAAC CTGTTTCTGA AGATGGTCCA GTCAGGTCGG CAGTTGCAGT AAGGCACCGG
    ACAACAGAAG AGCTTCGAGA GCTCTGGAAA AAGGCAATTC TTCAACAAAT TCTGCTTCTC
    AGGATGGAAA AAGAAAACCA AAAACTGCAA GCCTCAGAAA ATGACTTGCA GAATAAGAGG
    CTGAAACTTG ACTATGAAGA AATCACTCCA TGCCTGAAAG ATGTGACTTT AGTTTGGGAG
    AAGATGCTCA GCACACCCGG GAGATCAAAG ATCAAGACTG ACACAGAAAA AATACATGCA
    GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC CGTGGGGAGA TCTGGAAGTT TTTATCAGAA
    CAGTACCAGC TGAGACATCA GCATATAAGT AAGAACCAGT TAAAAGACAC CCCGTACAAG
    GAGCTTCTGA AGCAACTGAC CACCCAACAG CATGCAATAC TCATTGATCT AGGACGCACC
    TTTCCAACAC ACCCGTACTT CTCGGCCCAG CTGGGGGCCG GACAGCTGTC ACTCTACAAC
    ATTCTAAAGG CCTACTCCTT GCTGGATCCC GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT
    GTCGCTGGCG TCCTTTTGCT TCACATGACC GAGGAAGACG CCTTTAAGAT GCTAAAGTTT
    CTCATGTATG ACTTGGGTCT GAGGAAGCAG TATCGGCCCC ACATGATAAC TCTTCAGATC
    CAGATGTACC AGCTTTCCCG ACTCCTGCAC GACTATCACC GGGATCTGTA CAATCACTTG
    GAAACCTATG AGATCGGGCC CAGCCTGTAT GCTGCTCCTT GGTTCCTTAC CATGTTCGCC
    TCTCAGTTCC CGCTGGGATT TGTGGCGAGA GTCTTTGATA TGATTTTCCT TCAGGGATCA
    GAAGTCATAT TTAAGGTGGC CCTCAGCTTG CTCGGAAGCC ACAAGCCCTT GATTTTGCAG
    CATGAAAATT TGGAATCTAT TGTAGAATTT ATAAAGAACA CTCTTCCAAA CTTGGGTCTG
    GTGCAGATGG AAAAGACCAT TAACCAGGTG TTTGAAATGG ACATTTCCAA GCAGCTTCAG
    GCATATGAAG TGGAATATCA TGTACTGCAG GATGAACTGA TTGATTCATC TCCTCTCAGT
    GACAACCAGA GGATAAATAA ACTAGAGAAG GCAAATAGTA CATTGCGCAA ACAGAACTTT
    GACCTTTTGG AGGAGCTGCA GGTATCACAT GGGAGAATAC AGGTGCTAGA ATCCACCGTA
    GAAAACTTAC AGAGCAACGA GGTCAAGTTA AAACGATCCG TCCTTACTCT GGAGCTGGAA
    AGGTCTGCCT TGCTAAATAC CATCGAAGAA TTACGTGCTC AGATTAAAGA TATGCAAGGG
    AACAAGCTCT GTAATGACCA AAATGAGCCC AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910989.1
    CDS157..3627
    Translation

    Target ORF information:

    RefSeq Version XM_004910932.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910932.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGTCACCTC TGTTTAGCTG GGTGGCCAAG GTGCCGGAAA TAATTGGCTC TATCCGGCAT 
    GCAGGAAAGT TGGCACGGCA GGAAGAGGTT CATTCCCAGC TTTGTGATTT GGACGATTCA
    TTCTCCAAAA AGTTTGAAGT GCTGTTTTGT GGCCGCGTGA TTGTGGCGAA TAAGAAGGCA
    CCCCCTGCTT TGATAGACGA GTGCATTGAC AAGTTCAACC ATGTTAGCTG TACTAAAAAG
    AAGGAAGGGG ACTCTCTGAC TCCACAGGGG GACTCTCCAC CTCCACAACA GGACTCCGGC
    ACTGGGATAT CTTTCGGCGA TAAACTGCGC TCAGTCTTCC AATCGGCATT TCACGATGAT
    GACAAGAATA AGCCCATCCG CAAATCCTAC TCCCAGCCAG GCCTGCGGAC TAATACCTCT
    TTTACAAATG AATGGAAAGA TGGACGCAGT AGCCTTCCTA CTTCATTTGA GGAAAGCGAT
    ATAAGTTTGC ATGTACAAAG GTTATGTGGG GAGGAACAGG ACAGTGTGCA GCCAACAGAC
    ATGGAGGAAA ACCAGACTAT GCTATTCACG ATTGGCCAAG CAGAGGTTTA CCTGGTCAGC
    CCAGACACAA AGAGGATTGT GATTGAAAAG AGCTTTAAGG AGATATCATT TTGTTCTCAG
    GGAATCCAGC ATGCTGACCA CTTTGGTTTT ATCTGCAGGG AGCCATTGCT GAATGGAGGG
    TGCCATTTTG TGTGTTATGT ATTTCAGTGT GCAAATGGAG CACTGGTGGA TGAGATCATG
    CTGACTCTGA AGCAAGCCTT CAGTGTAGCT GCAGTGCAGC AGAACTCCAA GACTCTTCCG
    CAGCTCTGCG ATACTTGTCC CATGCAGTGC ATGCATAAAC TCTGTGAGAG AATAGAAGGT
    CTTCCTCCTA CCAAAATAAA ACTTGAACTG CAAAAGCACA TCACAACACT AAGCAATCAG
    GAGCAGGCCT CCATGTTTGA AGAAATACAG AAAATCCGGC CACGGTCAGA GCAGCAAGAA
    AATGAACTAG TCATCTCTCG CCTTCGATGT CTGTATGAGG AGAAACAGAG AGACCATATC
    CATAACGCTG AAACAAAGCA GAATTCGCAA GCAGCCGAAA GCACAGCCAA TGGGCTGCAG
    ACCAGTGCAT CTCGATTCAA ACTTGAGTCT CTGACCAGCA AAGCAAAGAG ATCTTTGACG
    GAATCTTTAG AAAACATTTT GTCAAGGGGC AGCAGGAGTC GCAGCCTGCA GGAGAGCTCT
    GCCAGTGGGG ATGATGACAG CTCACTATGC ACTCCTCTTG ATCCAAGTAT TAAGGAGCTG
    TCATTATGTG AGACAGAACC GGCTGCTCCG CTTGCAATCC CCTTAAAGAC TCACGGCTCC
    CTTGGAGATT TATCGAAGAG CTCAGATAGC TATTCCTGTG AGCATCCGGA GGAATTAGTT
    CAGCAGGGAT TCCGCCGGCG AGCTAACACA TTAAGCCATT TCCCTGTCAC AGCGCAGGAA
    TTTCCCGAGC CGGTTCAGAT AACTCCATCT GTTTCACAGC GGAAGCTAAT AAGGCACCAC
    TCTGTGAGCA CCGAGACGCC GCACAAAAGA AACACTCCTC TGCCTGAGCC GGATCACAAT
    CCAGTGCAGT CTGCAGAACC GACTGCTCCT CGTCCCGTCT CTGCAACCTC TTCCCCTGTA
    ACTGGGAAAA AACTGAAGGT CGGTTCTGTT GGGGAAGCAG GGGCGCCACT TTCACAAAAG
    AGCCTTTTCT ACCGTTCTAC TGCTCTTCGA AAGAAACTTC ATTCCTCATC TTCTGTGCCA
    AACTTTCTCA AATTTCTGGC CCCTGTAGCG GAAAATAGCA CCGCCGGCTT TAACAGTAGC
    AAAAGTGACT TTGAATGTAA GTCAATACAC AGCGAAGCTG GATGTGAGAG CCCAGTAAGG
    ACGCGCAGAC ATTCTTGGCG GCAACAGATC TTCTTACGTG TAGCCACCCC CCAAAAGGGC
    TGCGATTCCC CCAACAGATA TGATGACCAC TCAGATTTGG GGGACATTGC ACCCAGATCA
    CCTCTAGAAC CTGTTTCTGA AGATGGTCCA GTCAGGTCGG CAGTTGCAGT AAGGCACCGG
    ACAACAGAAG AGCTTCGAGA GCTCTGGAAA AAGGCAATTC TTCAACAAAT TCTGCTTCTC
    AGGATGGAAA AAGAAAACCA AAAACTGCAA GCCTCAGAAA ATGACTTGCA GAATAAGAGG
    CTGAAACTTG ACTATGAAGA AATCACTCCA TGCCTGAAAG ATGTGACTTT AGTTTGGGAG
    AAGATGCTCA GCACACCCGG GAGATCAAAG ATCAAGACTG ACACAGAAAA AATACATGCA
    GCTGTTGGAC AAGGGGTCCC CCGTCAGCAC CGTGGGGAGA TCTGGAAGTT TTTATCAGAA
    CAGTACCAGC TGAGACATCA GCATATAAGT AAGAACCAGT TAAAAGACAC CCCGTACAAG
    GAGCTTCTGA AGCAACTGAC CACCCAACAG CATGCAATAC TCATTGATCT AGGACGCACC
    TTTCCAACAC ACCCGTACTT CTCGGCCCAG CTGGGGGCCG GACAGCTGTC ACTCTACAAC
    ATTCTAAAGG CCTACTCCTT GCTGGATCCC GAAGTGGGCT ACTGCCAGGG CCTCAGCTTT
    GTCGCTGGCG TCCTTTTGCT TCACATGACC GAGGAAGACG CCTTTAAGAT GCTAAAGTTT
    CTCATGTATG ACTTGGGTCT GAGGAAGCAG TATCGGCCCC ACATGATAAC TCTTCAGATC
    CAGATGTACC AGCTTTCCCG ACTCCTGCAC GACTATCACC GGGATCTGTA CAATCACTTG
    GAAACCTATG AGATCGGGCC CAGCCTGTAT GCTGCTCCTT GGTTCCTTAC CATGTTCGCC
    TCTCAGTTCC CGCTGGGATT TGTGGCGAGA GTCTTTGATA TGATTTTCCT TCAGGGATCA
    GAAGTCATAT TTAAGGTGGC CCTCAGCTTG CTCGGAAGCC ACAAGCCCTT GATTTTGCAG
    CATGAAAATT TGGAATCTAT TGTAGAATTT ATAAAGAACA CTCTTCCAAA CTTGGGTCTG
    GTGCAGATGG AAAAGACCAT TAACCAGGTG TTTGAAATGG ACATTTCCAA GCAGCTTCAG
    GCATATGAAG TGGAATATCA TGTACTGCAG GATGAACTGA TTGATTCATC TCCTCTCAGT
    GACAACCAGA GGATAAATAA ACTAGAGAAG GCAAATAGTA CATTGCGCAA ACAGAACTTT
    GACCTTTTGG AGGAGCTGCA GGTATCACAT GGGAGAATAC AGGTGCTAGA ATCCACCGTA
    GAAAACTTAC AGAGCAACGA GGTCAAGTTA AAACGATCCG TCCTTACTCT GGAGCTGGAA
    AGGTCTGCCT TGCTAAATAC CATCGAAGAA TTACGTGCTC AGATTAAAGA TATGCAAGGG
    AACAAGCTCT GTAATGACCA AAATGAGCCC AACCAGCTCA ACAAGGAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04185
    Clone ID Related Accession (Same CDS sequence) XM_002938655.2
    Accession Version XM_002938655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3384bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 18, 2013 this sequence version replaced XM_002938655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACA CTCCTCTGCC TGAGCCGGAT CACAATCCAG TGCAGTCTGC AGAACCGACT
    GCTCCTCGTC CCGTCTCTGC AACCTCTTCC CCTGTAACTG GGAAAAAACT GAAGGTCGGT
    TCTGTTGGGG AAGCAGGGGC GCCACTTTCA CAAAAGAGTG ACTTTGAATG TAAGTCAATA
    CACAGCGAAG CTGGATGTGA GAGCCCAGTA AGGACGCGCA GACATTCTTG GCGGCAACAG
    ATCTTCTTAC GTGTAGCCAC CCCCCAAAAG GGCTGCGATT CCCCCAACAG ATATGATGAC
    CACTCAGATT TGGGGGACAT TGCACCCAGA TCACCTCTAG AACCTGTTTC TGAAGATGGT
    CCAGTCAGGT CGGCAGTTGC AGTAAGGCAC CGGACAACAG AAGAGCTTCG AGAGCTCTGG
    AAAAAGGCAA TTCTTCAACA AATTCTGCTT CTCAGGATGG AAAAAGAAAA CCAAAAACTG
    CAAGCCTCAG AAAATGACTT GCAGAATAAG AGGCTGAAAC TTGACTATGA AGAAATCACT
    CCATGCCTGA AAGATGTGAC TTTAGTTTGG GAGAAGATGC TCAGCACACC CGGGAGATCA
    AAGATCAAGA CTGACACAGA AAAAATACAT GCAGCTGTTG GACAAGGGGT CCCCCGTCAG
    CACCGTGGGG AGATCTGGAA GTTTTTATCA GAACAGTACC AGCTGAGACA TCAGCATATA
    AGTAAGAACC AGTTAAAAGA CACCCCGTAC AAGGAGCTTC TGAAGCAACT GACCACCCAA
    CAGCATGCAA TACTCATTGA TCTAGGACGC ACCTTTCCAA CACACCCGTA CTTCTCGGCC
    CAGCTGGGGG CCGGACAGCT GTCACTCTAC AACATTCTAA AGGCCTACTC CTTGCTGGAT
    CCCGAAGTGG GCTACTGCCA GGGCCTCAGC TTTGTCGCTG GCGTCCTTTT GCTTCACATG
    ACCGAGGAAG ACGCCTTTAA GATGCTAAAG TTTCTCATGT ATGACTTGGG TCTGAGGAAG
    CAGTATCGGC CCCACATGAT AACTCTTCAG ATCCAGATGT ACCAGCTTTC CCGACTCCTG
    CACGACTATC ACCGGGATCT GTACAATCAC TTGGAAACCT ATGAGATCGG GCCCAGCCTG
    TATGCTGCTC CTTGGTTCCT TACCATGTTC GCCTCTCAGT TCCCGCTGGG ATTTGTGGCG
    AGAGTCTTTG ATATGATTTT CCTTCAGGGA TCAGAAGTCA TATTTAAGGT GGCCCTCAGC
    TTGCTCGGAA GCCACAAGCC CTTGATTTTG CAGCATGAAA ATTTGGAATC TATTGTAGAA
    TTTATAAAGA ACACTCTTCC AAACTTGGGT CTGGTGCAGA TGGAAAAGAC CATTAACCAG
    GTGTTTGAAA TGGACATTTC CAAGCAGCTT CAGGCATATG AAGTGGAATA TCATGTACTG
    CAGGATGAAC TGATTGATTC ATCTCCTCTC AGTGACAACC AGAGGATAAA TAAACTAGAG
    AAGGCAAATA GTACATTGCG CAAACAGAAC TTTGACCTTT TGGAGGAGCT GCAGGTATCA
    CATGGGAGAA TACAGGTGCT AGAATCCACC GTAGAAAACT TACAGAGCAA CGAGGTCAAG
    TTAAAACGAT CCGTCCTTAC TCTGGAGCTG GAAAGGTCTG CCTTGCTAAA TACCATCGAA
    GAATTACGTG CTCAGATTAA AGATATGCAA GGGAACAAGC TCTGTAATGA CCAAAATGAG
    CCCAACCAGC TCAACAAGGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002938701.2
    CDS10..3393
    Translation

    Target ORF information:

    RefSeq Version XM_002938655.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002938655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAACA CTCCTCTGCC TGAGCCGGAT CACAATCCAG TGCAGTCTGC AGAACCGACT
    GCTCCTCGTC CCGTCTCTGC AACCTCTTCC CCTGTAACTG GGAAAAAACT GAAGGTCGGT
    TCTGTTGGGG AAGCAGGGGC GCCACTTTCA CAAAAGAGTG ACTTTGAATG TAAGTCAATA
    CACAGCGAAG CTGGATGTGA GAGCCCAGTA AGGACGCGCA GACATTCTTG GCGGCAACAG
    ATCTTCTTAC GTGTAGCCAC CCCCCAAAAG GGCTGCGATT CCCCCAACAG ATATGATGAC
    CACTCAGATT TGGGGGACAT TGCACCCAGA TCACCTCTAG AACCTGTTTC TGAAGATGGT
    CCAGTCAGGT CGGCAGTTGC AGTAAGGCAC CGGACAACAG AAGAGCTTCG AGAGCTCTGG
    AAAAAGGCAA TTCTTCAACA AATTCTGCTT CTCAGGATGG AAAAAGAAAA CCAAAAACTG
    CAAGCCTCAG AAAATGACTT GCAGAATAAG AGGCTGAAAC TTGACTATGA AGAAATCACT
    CCATGCCTGA AAGATGTGAC TTTAGTTTGG GAGAAGATGC TCAGCACACC CGGGAGATCA
    AAGATCAAGA CTGACACAGA AAAAATACAT GCAGCTGTTG GACAAGGGGT CCCCCGTCAG
    CACCGTGGGG AGATCTGGAA GTTTTTATCA GAACAGTACC AGCTGAGACA TCAGCATATA
    AGTAAGAACC AGTTAAAAGA CACCCCGTAC AAGGAGCTTC TGAAGCAACT GACCACCCAA
    CAGCATGCAA TACTCATTGA TCTAGGACGC ACCTTTCCAA CACACCCGTA CTTCTCGGCC
    CAGCTGGGGG CCGGACAGCT GTCACTCTAC AACATTCTAA AGGCCTACTC CTTGCTGGAT
    CCCGAAGTGG GCTACTGCCA GGGCCTCAGC TTTGTCGCTG GCGTCCTTTT GCTTCACATG
    ACCGAGGAAG ACGCCTTTAA GATGCTAAAG TTTCTCATGT ATGACTTGGG TCTGAGGAAG
    CAGTATCGGC CCCACATGAT AACTCTTCAG ATCCAGATGT ACCAGCTTTC CCGACTCCTG
    CACGACTATC ACCGGGATCT GTACAATCAC TTGGAAACCT ATGAGATCGG GCCCAGCCTG
    TATGCTGCTC CTTGGTTCCT TACCATGTTC GCCTCTCAGT TCCCGCTGGG ATTTGTGGCG
    AGAGTCTTTG ATATGATTTT CCTTCAGGGA TCAGAAGTCA TATTTAAGGT GGCCCTCAGC
    TTGCTCGGAA GCCACAAGCC CTTGATTTTG CAGCATGAAA ATTTGGAATC TATTGTAGAA
    TTTATAAAGA ACACTCTTCC AAACTTGGGT CTGGTGCAGA TGGAAAAGAC CATTAACCAG
    GTGTTTGAAA TGGACATTTC CAAGCAGCTT CAGGCATATG AAGTGGAATA TCATGTACTG
    CAGGATGAAC TGATTGATTC ATCTCCTCTC AGTGACAACC AGAGGATAAA TAAACTAGAG
    AAGGCAAATA GTACATTGCG CAAACAGAAC TTTGACCTTT TGGAGGAGCT GCAGGTATCA
    CATGGGAGAA TACAGGTGCT AGAATCCACC GTAGAAAACT TACAGAGCAA CGAGGTCAAG
    TTAAAACGAT CCGTCCTTAC TCTGGAGCTG GAAAGGTCTG CCTTGCTAAA TACCATCGAA
    GAATTACGTG CTCAGATTAA AGATATGCAA GGGAACAAGC TCTGTAATGA CCAAAATGAG
    CCCAACCAGC TCAACAAGGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa04186
    Clone ID Related Accession (Same CDS sequence) XM_004910933.2
    Accession Version XM_004910933.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3234bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product TBC1 domain family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 23, 2015 this sequence version replaced XM_004910933.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAATG ACTTTGAATG TAAGTCAATA CACAGCGAAG CTGGATGTGA GAGCCCAGTA
    AGGACGCGCA GACATTCTTG GCGGCAACAG ATCTTCTTAC GTGTAGCCAC CCCCCAAAAG
    GGCTGCGATT CCCCCAACAG ATATGATGAC CACTCAGATT TGGGGGACAT TGCACCCAGA
    TCACCTCTAG AACCTGTTTC TGAAGATGGT CCAGTCAGGT CGGCAGTTGC AGTAAGGCAC
    CGGACAACAG AAGAGCTTCG AGAGCTCTGG AAAAAGGCAA TTCTTCAACA AATTCTGCTT
    CTCAGGATGG AAAAAGAAAA CCAAAAACTG CAAGCCTCAG AAAATGACTT GCAGAATAAG
    AGGCTGAAAC TTGACTATGA AGAAATCACT CCATGCCTGA AAGATGTGAC TTTAGTTTGG
    GAGAAGATGC TCAGCACACC CGGGAGATCA AAGATCAAGA CTGACACAGA AAAAATACAT
    GCAGCTGTTG GACAAGGGGT CCCCCGTCAG CACCGTGGGG AGATCTGGAA GTTTTTATCA
    GAACAGTACC AGCTGAGACA TCAGCATATA AGTAAGAACC AGTTAAAAGA CACCCCGTAC
    AAGGAGCTTC TGAAGCAACT GACCACCCAA CAGCATGCAA TACTCATTGA TCTAGGACGC
    ACCTTTCCAA CACACCCGTA CTTCTCGGCC CAGCTGGGGG CCGGACAGCT GTCACTCTAC
    AACATTCTAA AGGCCTACTC CTTGCTGGAT CCCGAAGTGG GCTACTGCCA GGGCCTCAGC
    TTTGTCGCTG GCGTCCTTTT GCTTCACATG ACCGAGGAAG ACGCCTTTAA GATGCTAAAG
    TTTCTCATGT ATGACTTGGG TCTGAGGAAG CAGTATCGGC CCCACATGAT AACTCTTCAG
    ATCCAGATGT ACCAGCTTTC CCGACTCCTG CACGACTATC ACCGGGATCT GTACAATCAC
    TTGGAAACCT ATGAGATCGG GCCCAGCCTG TATGCTGCTC CTTGGTTCCT TACCATGTTC
    GCCTCTCAGT TCCCGCTGGG ATTTGTGGCG AGAGTCTTTG ATATGATTTT CCTTCAGGGA
    TCAGAAGTCA TATTTAAGGT GGCCCTCAGC TTGCTCGGAA GCCACAAGCC CTTGATTTTG
    CAGCATGAAA ATTTGGAATC TATTGTAGAA TTTATAAAGA ACACTCTTCC AAACTTGGGT
    CTGGTGCAGA TGGAAAAGAC CATTAACCAG GTGTTTGAAA TGGACATTTC CAAGCAGCTT
    CAGGCATATG AAGTGGAATA TCATGTACTG CAGGATGAAC TGATTGATTC ATCTCCTCTC
    AGTGACAACC AGAGGATAAA TAAACTAGAG AAGGCAAATA GTACATTGCG CAAACAGAAC
    TTTGACCTTT TGGAGGAGCT GCAGGTATCA CATGGGAGAA TACAGGTGCT AGAATCCACC
    GTAGAAAACT TACAGAGCAA CGAGGTCAAG TTAAAACGAT CCGTCCTTAC TCTGGAGCTG
    GAAAGGTCTG CCTTGCTAAA TACCATCGAA GAATTACGTG CTCAGATTAA AGATATGCAA
    GGGAACAAGC TCTGTAATGA CCAAAATGAG CCCAACCAGC TCAACAAGGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910990.1
    CDS16..3249
    Translation

    Target ORF information:

    RefSeq Version XM_004910933.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis TBC1 domain family member 1 (tbc1d1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910933.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGAAGGCCA GGCTGCAGAA ACTCATCTCC ATTCACCTGG ATACAGGGCA AGAGAAAGAG 
    CTGAAGGTGC CGGAAATAAT TGGCTCTATC CGGCATGCAG GAAAGTTGGC ACGGCAGGAA
    GAGGTTCATT CCCAGCTTTG TGATTTGGAC GATTCATTCT CCAAAAAGTT TGAAGTGCTG
    TTTTGTGGCC GCGTGATTGT GGCGAATAAG AAGGCACCCC CTGCTTTGAT AGACGAGTGC
    ATTGACAAGT TCAACCATGT TAGCTGTACT AAAAAGAAGG AAGGGGACTC TCTGACTCCA
    CAGGGGGACT CTCCACCTCC ACAACAGGAC TCCGGCACTG GGATATCTTT CGGCGATAAA
    CTGCGCTCAG TCTTCCAATC GGCATTTCAC GATGATGACA AGAATAAGCC CATCCGCAAA
    TCCTACTCCC AGCCAGGCCT GCGGACTAAT ACCTCTTTTA CAAATGAATG GAAAGATGGA
    CGCAGTAGCC TTCCTACTTC ATTTGAGGAA AGCGATATAA GTTTGCATGT ACAAAGGTTA
    TGTGGGGAGG AACAGGACAG TGTGCAGCCA ACAGACATGG AGGAAAACCA GACTATGCTA
    TTCACGATTG GCCAAGCAGA GGTTTACCTG GTCAGCCCAG ACACAAAGAG GATTGTGATT
    GAAAAGAGCT TTAAGGAGAT ATCATTTTGT TCTCAGGGAA TCCAGCATGC TGACCACTTT
    GGTTTTATCT GCAGGGAGCC ATTGCTGAAT GGAGGGTGCC ATTTTGTGTG TTATGTATTT
    CAGTGTGCAA ATGGAGCACT GGTGGATGAG ATCATGCTGA CTCTGAAGCA AGCCTTCAGT
    GTAGCTGCAG TGCAGCAGAA CTCCAAGACT CTTCCGCAGC TCTGCGATAC TTGTCCCATG
    CAGTGCATGC ATAAACTCTG TGAGAGAATA GAAGGTCTTC CTCCTACCAA AATAAAACTT
    GAACTGCAAA AGCACATCAC AACACTAAGC AATCAGGAGC AGGCCTCCAT GTTTGAAGAA
    ATACAGAAAA TCCGGCCACG GTCAGAGCAG CAAGAAAATG AACTAGTCAT CTCTCGCCTT
    CGATGTCTGT ATGAGGAGAA ACAGAGAGAC CATATCCATA ACGCTGAAAC AAAGCAGAAT
    TCGCAAGCAG CCGAAAGCAC AGCCAATGGG CTGCAGACCA GTGCATCTCG ATTCAAACTT
    GAGTCTCTGA CCAGCAAAGC AAAGAGATCT TTGACGGAAT CTTTAGAAAA CATTTTGTCA
    AGGGGCAGCA GGAGTCGCAG CCTGCAGGAG AGCTCTGCCA GTGGGGATGA TGACAGCTCA
    CTATGCACTC CTCTTGATCC AAGTATTAAG GAGCTGTCAT TATGTGAGAC AGAACCGGCT
    GCTCCGCTTG CAATCCCCTT AAAGACTCAC GGCTCCCTTG GAGATTTATC GAAGAGCTCA
    GATAGCTATT CCTGTGAGCA TCCGGAGGAA TTAGTTCAGC AGGGATTCCG CCGGCGAGCT
    AACACATTAA GCCATTTCCC TGTCACAGCG CAGGAATTTC CCGAGCCGGT TCAGATAACT
    CCATCTGTTT CACAGCGGAA GCTAATAAGG CACCACTCTG TGAGCACCGA GACGCCGCAC
    AAAAGAAATG ACTTTGAATG TAAGTCAATA CACAGCGAAG CTGGATGTGA GAGCCCAGTA
    AGGACGCGCA GACATTCTTG GCGGCAACAG ATCTTCTTAC GTGTAGCCAC CCCCCAAAAG
    GGCTGCGATT CCCCCAACAG ATATGATGAC CACTCAGATT TGGGGGACAT TGCACCCAGA
    TCACCTCTAG AACCTGTTTC TGAAGATGGT CCAGTCAGGT CGGCAGTTGC AGTAAGGCAC
    CGGACAACAG AAGAGCTTCG AGAGCTCTGG AAAAAGGCAA TTCTTCAACA AATTCTGCTT
    CTCAGGATGG AAAAAGAAAA CCAAAAACTG CAAGCCTCAG AAAATGACTT GCAGAATAAG
    AGGCTGAAAC TTGACTATGA AGAAATCACT CCATGCCTGA AAGATGTGAC TTTAGTTTGG
    GAGAAGATGC TCAGCACACC CGGGAGATCA AAGATCAAGA CTGACACAGA AAAAATACAT
    GCAGCTGTTG GACAAGGGGT CCCCCGTCAG CACCGTGGGG AGATCTGGAA GTTTTTATCA
    GAACAGTACC AGCTGAGACA TCAGCATATA AGTAAGAACC AGTTAAAAGA CACCCCGTAC
    AAGGAGCTTC TGAAGCAACT GACCACCCAA CAGCATGCAA TACTCATTGA TCTAGGACGC
    ACCTTTCCAA CACACCCGTA CTTCTCGGCC CAGCTGGGGG CCGGACAGCT GTCACTCTAC
    AACATTCTAA AGGCCTACTC CTTGCTGGAT CCCGAAGTGG GCTACTGCCA GGGCCTCAGC
    TTTGTCGCTG GCGTCCTTTT GCTTCACATG ACCGAGGAAG ACGCCTTTAA GATGCTAAAG
    TTTCTCATGT ATGACTTGGG TCTGAGGAAG CAGTATCGGC CCCACATGAT AACTCTTCAG
    ATCCAGATGT ACCAGCTTTC CCGACTCCTG CACGACTATC ACCGGGATCT GTACAATCAC
    TTGGAAACCT ATGAGATCGG GCCCAGCCTG TATGCTGCTC CTTGGTTCCT TACCATGTTC
    GCCTCTCAGT TCCCGCTGGG ATTTGTGGCG AGAGTCTTTG ATATGATTTT CCTTCAGGGA
    TCAGAAGTCA TATTTAAGGT GGCCCTCAGC TTGCTCGGAA GCCACAAGCC CTTGATTTTG
    CAGCATGAAA ATTTGGAATC TATTGTAGAA TTTATAAAGA ACACTCTTCC AAACTTGGGT
    CTGGTGCAGA TGGAAAAGAC CATTAACCAG GTGTTTGAAA TGGACATTTC CAAGCAGCTT
    CAGGCATATG AAGTGGAATA TCATGTACTG CAGGATGAAC TGATTGATTC ATCTCCTCTC
    AGTGACAACC AGAGGATAAA TAAACTAGAG AAGGCAAATA GTACATTGCG CAAACAGAAC
    TTTGACCTTT TGGAGGAGCT GCAGGTATCA CATGGGAGAA TACAGGTGCT AGAATCCACC
    GTAGAAAACT TACAGAGCAA CGAGGTCAAG TTAAAACGAT CCGTCCTTAC TCTGGAGCTG
    GAAAGGTCTG CCTTGCTAAA TACCATCGAA GAATTACGTG CTCAGATTAA AGATATGCAA
    GGGAACAAGC TCTGTAATGA CCAAAATGAG CCCAACCAGC TCAACAAGGA ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.