TBC1D1 cDNA ORF clone, Bos taurus(cattle)

The following TBC1D1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the TBC1D1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa20341 XM_010806057.3
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa20341 XM_005207843.3
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa235162 XM_024993034.1
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 9-11 $69.30
$99.00
OBa235160 XM_024993032.1
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa235161 XM_024993033.1
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa20337 NM_001166524.1
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20334 XM_005207835.4
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20335 XM_005207836.4
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20337 XM_005207838.4
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20338 XM_005207839.4
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20339 XM_005207840.4
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20340 XM_005207841.4
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45492 XM_010806055.2
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa45493 XM_010806056.3
Latest version!
Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
OBa20337 XM_005207838.3 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20340 XM_005207841.3 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20335 XM_005207836.3 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20339 XM_005207840.3 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa45493 XM_010806056.2 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
OBa20341 XM_010806057.2 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $258.30
$369.00
OBa20334 XM_005207835.3 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
OBa20338 XM_005207839.3 Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa20341
    Clone ID Related Accession (Same CDS sequence) XM_010806057.2 , XM_005207843.3 , XM_010806057.3
    Accession Version XM_010806057.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806057.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA 
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804359.1
    CDS184..1482
    Translation

    Target ORF information:

    RefSeq Version XM_010806057.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806057.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA 
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20341
    Clone ID Related Accession (Same CDS sequence) XM_010806057.2 , XM_005207843.3 , XM_010806057.3
    Accession Version XM_005207843.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207843.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA 
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207900.1
    CDS349..1647
    Translation

    Target ORF information:

    RefSeq Version XM_005207843.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207843.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA 
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20341
    Clone ID Related Accession (Same CDS sequence) XM_010806057.2 , XM_005207843.3 , XM_010806057.3
    Accession Version XM_010806057.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1299bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806057.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA 
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804359.1
    CDS183..1481
    Translation

    Target ORF information:

    RefSeq Version XM_010806057.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806057.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA 
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235162
    Clone ID Related Accession (Same CDS sequence) XM_024993034.1
    Accession Version XM_024993034.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 552bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCAGGATA TGATCTTTCT TCAGGGATCA GAGGTCATAT TTAAAGTGGC TTTAAGTCTG 
    CTGGGAAGCC ATAAGCCCTT GATTCTGCAG CATGAAAACC TAGAAACCAT AGTTGACTTT
    ATAAAAAGCA CACTACCCAA CCTGGGCTTG GTGCAGATGG AGAAGACTAT CAGTCAGGTG
    TTTGAGACGG ACATCTCGAA ACAGTTACAA GCTTATGAAG TTGAGTACCA CGTGCTTCAG
    GAAGAGCTTA TCGATTCTTC TCCTCTCAGT GACAACCAAA GAATGGACAA ACTAGAGAAG
    ACCAACAGCA GCTTACGCAA ACAGAACCTT GACCTCCTTG AACAGCTGCA GGTCGCCAAT
    GGGAGGATCC AGAGCCTGGA AGCCACTGTT GAGAAGCTCC TGACTAGCGA GAGCAAGCTG
    AAGCAGGCGA CCCTGGCCTT GGAGCTGGAG AGGTCAGCCC TGCTGCAGAC GGTGGAGCAG
    CTCCGGAGGC AGACGGCAGA GCTGGGCAGC CAGGAGTCTG ACCCCACACT GCCCAAGCCC
    TCAGGTGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848802.1
    CDS177..728
    Translation

    Target ORF information:

    RefSeq Version XM_024993034.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993034.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    ATGCAGGATA TGATCTTTCT TCAGGGATCA GAGGTCATAT TTAAAGTGGC TTTAAGTCTG 
    CTGGGAAGCC ATAAGCCCTT GATTCTGCAG CATGAAAACC TAGAAACCAT AGTTGACTTT
    ATAAAAAGCA CACTACCCAA CCTGGGCTTG GTGCAGATGG AGAAGACTAT CAGTCAGGTG
    TTTGAGACGG ACATCTCGAA ACAGTTACAA GCTTATGAAG TTGAGTACCA CGTGCTTCAG
    GAAGAGCTTA TCGATTCTTC TCCTCTCAGT GACAACCAAA GAATGGACAA ACTAGAGAAG
    ACCAACAGCA GCTTACGCAA ACAGAACCTT GACCTCCTTG AACAGCTGCA GGTCGCCAAT
    GGGAGGATCC AGAGCCTGGA AGCCACTGTT GAGAAGCTCC TGACTAGCGA GAGCAAGCTG
    AAGCAGGCGA CCCTGGCCTT GGAGCTGGAG AGGTCAGCCC TGCTGCAGAC GGTGGAGCAG
    CTCCGGAGGC AGACGGCAGA GCTGGGCAGC CAGGAGTCTG ACCCCACACT GCCCAAGCCC
    TCAGGTGACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235160
    Clone ID Related Accession (Same CDS sequence) XM_024993032.1
    Accession Version XM_024993032.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3204bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCTCACG TTGCTGAAAT CTCCAGGCTG GCAGGAACTG AAGGTTCTTT CTCCACCGCG 
    CCTTCTCTAG TCTCAACATT TGAGTTTTCT GACTACAAGA AAACCACCTT TGATTATATA
    AAGGTGCCTG AGATCATCAG TTCCATCCGG CAGGCTGGGA AGATCGCCCG CCAGGAGGAG
    CTCCGCTGCC CTTCGGAGTT CGATGACACA TTCGCCAAGA AGTTCGAGGT GCTGTTCTGC
    GGCCGCGTGG CCGTGGCGCA CAGGAAGGCT CCACCCGCCC TGATCGATGA GTGCATCGAA
    GGCTTCAGTC ACGTGAGCGG TGGCTTCTCC TCGGATCAGT CCCGGTCTGC ACTGCAGCCA
    CCGGGGGATG GGGAGCGGGG CCCGCGGCCG ATGCGCAAGT CCTTCTCGCA GCCCGGGCTG
    CGCTCCTTGG CTTTCCGAAA GGAGTTTCAG GATGCAGGCC TGCGGAGTAG TAGTTTCTTC
    AGCTCTTTTG AGGAGAGCGA CATCGAGAAC CACCTGATTA GTGGACACAA TATTGTGCAG
    CCGACAGATA TTGAGGAAAA TCGAACTATG CTCTTCACGA TTGGTCAATC TGAAGTTTAC
    CTCATCAGTC CTGACACCAA AAAAATAGCG TTGCAGAAAA ATTTTAAGGA GATATCCTTT
    TGCTCTCAGG GTATTAGACA TGTGGACCAC TTTGGGTTCA TCTGCCGTGA GTCTTCCGGA
    GGTGGAGGCT TTCATTTCGT CTGTTACGTG TTTCAGTGCA CAAATGAAGC TCTGGTGGAT
    GAGATCATGA TGACCTTGAA GCAGGCCTTC ACGGTGGCTG CTGTGCAGCA GACAGCCAAG
    GCGCCAGCCC AGCTGTGTGA GGGCTGCCCC CTGCAGGGCC TGCACAAGCT CTGCGAGCGG
    ATCGAGGGAA TGAATTCTTC CAAAACCAAA CTAGAACTCC AGAAGCACCT GACAACATTA
    ACTAATCAGG AGCAAGCAAC TATTTTTGAG GAGGTTCAGA AATTGAGACC AAGAAATGAG
    CAACGAGAGA ATGAATTGAT TATTTCTTTC CTGAGATGTT TATATGAAGA GAAGCAAAAA
    GTTCACATCC ATATTGGGGA GATAAAACAG ACGTCACAGA TTGCAGCAGA GAATATTGGA
    AGCGAATTAC CATCAAGTGC CACTCGATTT AGGCTAGATA TGCTGAAAAA CAAAGCGAAG
    AGGTCTTTAA CAGAATCTTT AGAAAGTATT TTGTCTCGGG GTAATAAAGC CAGAGGCCTC
    CAGGAACACT CAGCCAGTTT GGATCTGGAC AGCTCCGTGT CTAGCATGTT CAGTAACACC
    AGCAAAGAGC CGTCTGGCTA TGAAAAGGAG GCCTTGCCCA TCTCCGAGAG CTGCTTCAGG
    CTCCTCGGCT CCTCGGACGA CCTGTCCAGT GACTCAGAGA GCCAACTCAC AGAAGAGCCA
    GCCCTGCTCT CGCCCAAGCA GGGCTTCCGA AGGCGCGCCA ACACCCTGAG TCATGTTCCC
    GTGGAATGCC AGGAGCCTCC ACAGCTGGTG CGAGGGTCTC CAGGGGTCTC GCAAAGGAAA
    CTTGTGAGGT ATCACTCAGT GAGCACAGAG ACGCCTCATG AACGAAAGGA CTTTGAGTCC
    AAAGCCGACC ACATCAGCGA TGCCAGCAGG ACCCCTGTGA AGACACGGAG ACACTCGTGG
    AGGCAGCAGA TCTTCCTTCG GGTAGCCACC CCCCAGAAGG CCTGCGAGTC CCCCAAAAGA
    TACGAAGATT ACTCAGAGTT GGGAGAGCTT CCCCCACGAT CTCCACTAGA ACCAGTTTGT
    GAAGATGGAC CCTTTGGCCC GGTCCCAGAG GAAAAGAAAA GAACATCTCA TGAACTTCGG
    GAGCTGTGGC AAAAGGCCAT TCTACAACAG ATATTGCTGC TCAGGATGGA GAAAGAAAAC
    CAAAAACTGC AAGCCTCTGA GAATGATTTG CTGAACAAGC GCCTAAAGCT TGATTATGAA
    GAAATCACTC CCTGTCTTAA AGAAGTGACT ACAGTGTGGG AAAAGATACT TAGCACTCCA
    GGAAGATCAA AAATTAAGTT TGACATGGAA AAAATGCACT CGGCTGTTGG CCAAGGTGTG
    CCGCGACACC ACCGGGGTGA AATCTGGAAA TTCTTGGCGG AGCAGTACCA CCTGAAGCAC
    CCGTTTCCCT GCAAACAGCA GCCCAAGGAC ACGCCCTACA AAGAGCTCTT AAAGCAGCTC
    ACCTCCCAGC AGCACGCCAT TCTCATCGAC CTTGGGCGAA CCTTTCCAAC ACATCCGTAC
    TACTCTGCTC AGCTAGGAGC AGGGCAGCTG TCACTTTACA ACATCCTGAA GGCCTACTCG
    CTTCTAGACC AAGAAGTGGG CTATTGCCAA GGTCTCAGCT TTGTAGCAGG CATCCTGCTG
    CTTCATATGG GTGAAGAAGA GGCGTTTAAC ATGCTCAAGT TTCTGATGTT TGACATGGGA
    CTGCGGAAAC AATATCGGCC GGACATGATT ATTTTACAGA TCCAGATGTA CCAGCTCTCG
    AGGCTGCTCC ACGACTACCA CAGGGACCTC TACAACCACC TGGAGGAGCA TGAGATTGGC
    CCCAGCCTCT ATGCTGCGCC CTGGTTCCTC ACCGTGTTTG CCTCACAGTT CCCCCTGGGC
    TTCGTCGCCA GAGTCTTTGA TATGATCTTT CTTCAGGGAT CAGAGGTCAT ATTTAAAGTG
    GCTTTAAGTC TGCTGGGAAG CCATAAGCCC TTGATTCTGC AGCATGAAAA CCTAGAAACC
    ATAGTTGACT TTATAAAAAG CACACTACCC AACCTGGGCT TGGTGCAGAT GGAGAAGACT
    ATCAGTCAGG TGTTTGAGAC GGACATCTCG AAACAGTTAC AAGCTTATGA AGTTGAGTAC
    CACGTGCTTC AGGAAGAGCT TATCGATTCT TCTCCTCTCA GTGACAACCA AAGAATGGAC
    AAACTAGAGA AGACCAACAG CAGCTTACGC AAACAGAACC TTGACCTCCT TGAACAGCTG
    CAGGTCGCCA ATGGGAGGAT CCAGAGCCTG GAAGCCACTG TTGAGAAGCT CCTGACTAGC
    GAGAGCAAGC TGAAGCAGGC GACCCTGGCC TTGGAGCTGG AGAGGTCAGC CCTGCTGCAG
    ACGGTGGAGC AGCTCCGGAG GCAGACGGCA GAGCTGGGCA GCCAGGAGTC TGACCCCACA
    CTGCCCAAGC CCTCAGGTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848800.1
    CDS22..3225
    Translation

    Target ORF information:

    RefSeq Version XM_024993032.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993032.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGTCTCACG TTGCTGAAAT CTCCAGGCTG GCAGGAACTG AAGGTTCTTT CTCCACCGCG 
    CCTTCTCTAG TCTCAACATT TGAGTTTTCT GACTACAAGA AAACCACCTT TGATTATATA
    AAGGTGCCTG AGATCATCAG TTCCATCCGG CAGGCTGGGA AGATCGCCCG CCAGGAGGAG
    CTCCGCTGCC CTTCGGAGTT CGATGACACA TTCGCCAAGA AGTTCGAGGT GCTGTTCTGC
    GGCCGCGTGG CCGTGGCGCA CAGGAAGGCT CCACCCGCCC TGATCGATGA GTGCATCGAA
    GGCTTCAGTC ACGTGAGCGG TGGCTTCTCC TCGGATCAGT CCCGGTCTGC ACTGCAGCCA
    CCGGGGGATG GGGAGCGGGG CCCGCGGCCG ATGCGCAAGT CCTTCTCGCA GCCCGGGCTG
    CGCTCCTTGG CTTTCCGAAA GGAGTTTCAG GATGCAGGCC TGCGGAGTAG TAGTTTCTTC
    AGCTCTTTTG AGGAGAGCGA CATCGAGAAC CACCTGATTA GTGGACACAA TATTGTGCAG
    CCGACAGATA TTGAGGAAAA TCGAACTATG CTCTTCACGA TTGGTCAATC TGAAGTTTAC
    CTCATCAGTC CTGACACCAA AAAAATAGCG TTGCAGAAAA ATTTTAAGGA GATATCCTTT
    TGCTCTCAGG GTATTAGACA TGTGGACCAC TTTGGGTTCA TCTGCCGTGA GTCTTCCGGA
    GGTGGAGGCT TTCATTTCGT CTGTTACGTG TTTCAGTGCA CAAATGAAGC TCTGGTGGAT
    GAGATCATGA TGACCTTGAA GCAGGCCTTC ACGGTGGCTG CTGTGCAGCA GACAGCCAAG
    GCGCCAGCCC AGCTGTGTGA GGGCTGCCCC CTGCAGGGCC TGCACAAGCT CTGCGAGCGG
    ATCGAGGGAA TGAATTCTTC CAAAACCAAA CTAGAACTCC AGAAGCACCT GACAACATTA
    ACTAATCAGG AGCAAGCAAC TATTTTTGAG GAGGTTCAGA AATTGAGACC AAGAAATGAG
    CAACGAGAGA ATGAATTGAT TATTTCTTTC CTGAGATGTT TATATGAAGA GAAGCAAAAA
    GTTCACATCC ATATTGGGGA GATAAAACAG ACGTCACAGA TTGCAGCAGA GAATATTGGA
    AGCGAATTAC CATCAAGTGC CACTCGATTT AGGCTAGATA TGCTGAAAAA CAAAGCGAAG
    AGGTCTTTAA CAGAATCTTT AGAAAGTATT TTGTCTCGGG GTAATAAAGC CAGAGGCCTC
    CAGGAACACT CAGCCAGTTT GGATCTGGAC AGCTCCGTGT CTAGCATGTT CAGTAACACC
    AGCAAAGAGC CGTCTGGCTA TGAAAAGGAG GCCTTGCCCA TCTCCGAGAG CTGCTTCAGG
    CTCCTCGGCT CCTCGGACGA CCTGTCCAGT GACTCAGAGA GCCAACTCAC AGAAGAGCCA
    GCCCTGCTCT CGCCCAAGCA GGGCTTCCGA AGGCGCGCCA ACACCCTGAG TCATGTTCCC
    GTGGAATGCC AGGAGCCTCC ACAGCTGGTG CGAGGGTCTC CAGGGGTCTC GCAAAGGAAA
    CTTGTGAGGT ATCACTCAGT GAGCACAGAG ACGCCTCATG AACGAAAGGA CTTTGAGTCC
    AAAGCCGACC ACATCAGCGA TGCCAGCAGG ACCCCTGTGA AGACACGGAG ACACTCGTGG
    AGGCAGCAGA TCTTCCTTCG GGTAGCCACC CCCCAGAAGG CCTGCGAGTC CCCCAAAAGA
    TACGAAGATT ACTCAGAGTT GGGAGAGCTT CCCCCACGAT CTCCACTAGA ACCAGTTTGT
    GAAGATGGAC CCTTTGGCCC GGTCCCAGAG GAAAAGAAAA GAACATCTCA TGAACTTCGG
    GAGCTGTGGC AAAAGGCCAT TCTACAACAG ATATTGCTGC TCAGGATGGA GAAAGAAAAC
    CAAAAACTGC AAGCCTCTGA GAATGATTTG CTGAACAAGC GCCTAAAGCT TGATTATGAA
    GAAATCACTC CCTGTCTTAA AGAAGTGACT ACAGTGTGGG AAAAGATACT TAGCACTCCA
    GGAAGATCAA AAATTAAGTT TGACATGGAA AAAATGCACT CGGCTGTTGG CCAAGGTGTG
    CCGCGACACC ACCGGGGTGA AATCTGGAAA TTCTTGGCGG AGCAGTACCA CCTGAAGCAC
    CCGTTTCCCT GCAAACAGCA GCCCAAGGAC ACGCCCTACA AAGAGCTCTT AAAGCAGCTC
    ACCTCCCAGC AGCACGCCAT TCTCATCGAC CTTGGGCGAA CCTTTCCAAC ACATCCGTAC
    TACTCTGCTC AGCTAGGAGC AGGGCAGCTG TCACTTTACA ACATCCTGAA GGCCTACTCG
    CTTCTAGACC AAGAAGTGGG CTATTGCCAA GGTCTCAGCT TTGTAGCAGG CATCCTGCTG
    CTTCATATGG GTGAAGAAGA GGCGTTTAAC ATGCTCAAGT TTCTGATGTT TGACATGGGA
    CTGCGGAAAC AATATCGGCC GGACATGATT ATTTTACAGA TCCAGATGTA CCAGCTCTCG
    AGGCTGCTCC ACGACTACCA CAGGGACCTC TACAACCACC TGGAGGAGCA TGAGATTGGC
    CCCAGCCTCT ATGCTGCGCC CTGGTTCCTC ACCGTGTTTG CCTCACAGTT CCCCCTGGGC
    TTCGTCGCCA GAGTCTTTGA TATGATCTTT CTTCAGGGAT CAGAGGTCAT ATTTAAAGTG
    GCTTTAAGTC TGCTGGGAAG CCATAAGCCC TTGATTCTGC AGCATGAAAA CCTAGAAACC
    ATAGTTGACT TTATAAAAAG CACACTACCC AACCTGGGCT TGGTGCAGAT GGAGAAGACT
    ATCAGTCAGG TGTTTGAGAC GGACATCTCG AAACAGTTAC AAGCTTATGA AGTTGAGTAC
    CACGTGCTTC AGGAAGAGCT TATCGATTCT TCTCCTCTCA GTGACAACCA AAGAATGGAC
    AAACTAGAGA AGACCAACAG CAGCTTACGC AAACAGAACC TTGACCTCCT TGAACAGCTG
    CAGGTCGCCA ATGGGAGGAT CCAGAGCCTG GAAGCCACTG TTGAGAAGCT CCTGACTAGC
    GAGAGCAAGC TGAAGCAGGC GACCCTGGCC TTGGAGCTGG AGAGGTCAGC CCTGCTGCAG
    ACGGTGGAGC AGCTCCGGAG GCAGACGGCA GAGCTGGGCA GCCAGGAGTC TGACCCCACA
    CTGCCCAAGC CCTCAGGTGA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa235161
    Clone ID Related Accession (Same CDS sequence) XM_024993033.1
    Accession Version XM_024993033.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3111bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCACCAT TGTTCAGCTG GGTGGCCAAG GTGCCTGAGA TCATCAGTTC CATCCGGCAG 
    GCTGGGAAGA TCGCCCGCCA GGAGGAGCTC CGCTGCCCTT CGGAGTTCGA TGACACATTC
    GCCAAGAAGT TCGAGGTGCT GTTCTGCGGC CGCGTGGCCG TGGCGCACAG GAAGGCTCCA
    CCCGCCCTGA TCGATGAGTG CATCGAAGGC TTCAGTCACG TGAGCGGTGG CTTCTCCTCG
    GATCAGTCCC GGTCTGCACT GCAGCCACCG GGGGATGGGG AGCGGGGCCC GCGGCCGATG
    CGCAAGTCCT TCTCGCAGCC CGGGCTGCGC TCCTTGGCTT TCCGAAAGGA GTTTCAGGAT
    GCAGGCCTGC GGAGTAGTAG TTTCTTCAGC TCTTTTGAGG AGAGCGACAT CGAGAACCAC
    CTGATTAGTG GACACAATAT TGTGCAGCCG ACAGATATTG AGGAAAATCG AACTATGCTC
    TTCACGATTG GTCAATCTGA AGTTTACCTC ATCAGTCCTG ACACCAAAAA AATAGCGTTG
    CAGAAAAATT TTAAGGAGAT ATCCTTTTGC TCTCAGGGTA TTAGACATGT GGACCACTTT
    GGGTTCATCT GCCGTGAGTC TTCCGGAGGT GGAGGCTTTC ATTTCGTCTG TTACGTGTTT
    CAGTGCACAA ATGAAGCTCT GGTGGATGAG ATCATGATGA CCTTGAAGCA GGCCTTCACG
    GTGGCTGCTG TGCAGCAGAC AGCCAAGGCG CCAGCCCAGC TGTGTGAGGG CTGCCCCCTG
    CAGGGCCTGC ACAAGCTCTG CGAGCGGATC GAGGGAATGA ATTCTTCCAA AACCAAACTA
    GAACTCCAGA AGCACCTGAC AACATTAACT AATCAGGAGC AAGCAACTAT TTTTGAGGAG
    GTTCAGAAAT TGAGACCAAG AAATGAGCAA CGAGAGAATG AATTGATTAT TTCTTTCCTG
    AGATGTTTAT ATGAAGAGAA GCAAAAAGTT CACATCCATA TTGGGGAGAT AAAACAGACG
    TCACAGATTG CAGCAGAGAA TATTGGAAGC GAATTACCAT CAAGTGCCAC TCGATTTAGG
    CTAGATATGC TGAAAAACAA AGCGAAGAGG TCTTTAACAG AATCTTTAGA AAGTATTTTG
    TCTCGGGGTA ATAAAGCCAG AGGCCTCCAG GAACACTCAG CCAGTTTGGA TCTGGACAGC
    TCCGTGTCTA GCATGTTCAG TAACACCAGC AAAGAGCCGT CTGGCTATGA AAAGGAGGCC
    TTGCCCATCT CCGAGAGCTG CTTCAGGCTC CTCGGCTCCT CGGACGACCT GTCCAGTGAC
    TCAGAGAGCC AACTCACAGA AGAGCCAGCC CTGCTCTCGC CCAAGCAGGG CTTCCGAAGG
    CGCGCCAACA CCCTGAGTCA TGTTCCCGTG GAATGCCAGG AGCCTCCACA GCTGGTGCGA
    GGGTCTCCAG GGGTCTCGCA AAGGAAACTT GTGAGGTATC ACTCAGTGAG CACAGAGACG
    CCTCATGAAC GAAAGGACTT TGAGTCCAAA GCCGACCACA TCAGCGATGC CAGCAGGACC
    CCTGTGAAGA CACGGAGACA CTCGTGGAGG CAGCAGATCT TCCTTCGGGT AGCCACCCCC
    CAGAAGGCCT GCGAGTCCCC CAAAAGATAC GAAGATTACT CAGAGTTGGG AGAGCTTCCC
    CCACGATCTC CACTAGAACC AGTTTGTGAA GATGGACCCT TTGGCCCGGT CCCAGAGGAA
    AAGAAAAGAA CATCTCATGA ACTTCGGGAG CTGTGGCAAA AGGCCATTCT ACAACAGATA
    TTGCTGCTCA GGATGGAGAA AGAAAACCAA AAACTGCAAG CCTCTGAGAA TGATTTGCTG
    AACAAGCGCC TAAAGCTTGA TTATGAAGAA ATCACTCCCT GTCTTAAAGA AGTGACTACA
    GTGTGGGAAA AGATACTTAG CACTCCAGGA AGATCAAAAA TTAAGTTTGA CATGGAAAAA
    ATGCACTCGG CTGTTGGCCA AGGTGTGCCG CGACACCACC GGGGTGAAAT CTGGAAATTC
    TTGGCGGAGC AGTACCACCT GAAGCACCCG TTTCCCTGCA AACAGCAGCC CAAGGACACG
    CCCTACAAAG AGCTCTTAAA GCAGCTCACC TCCCAGCAGC ACGCCATTCT CATCGACCTT
    GGGCGAACCT TTCCAACACA TCCGTACTAC TCTGCTCAGC TAGGAGCAGG GCAGCTGTCA
    CTTTACAACA TCCTGAAGGC CTACTCGCTT CTAGACCAAG AAGTGGGCTA TTGCCAAGGT
    CTCAGCTTTG TAGCAGGCAT CCTGCTGCTT CATATGGGTG AAGAAGAGGC GTTTAACATG
    CTCAAGTTTC TGATGTTTGA CATGGGACTG CGGAAACAAT ATCGGCCGGA CATGATTATT
    TTACAGATCC AGATGTACCA GCTCTCGAGG CTGCTCCACG ACTACCACAG GGACCTCTAC
    AACCACCTGG AGGAGCATGA GATTGGCCCC AGCCTCTATG CTGCGCCCTG GTTCCTCACC
    GTGTTTGCCT CACAGTTCCC CCTGGGCTTC GTCGCCAGAG TCTTTGATAT GATCTTTCTT
    CAGGGATCAG AGGTCATATT TAAAGTGGCT TTAAGTCTGC TGGGAAGCCA TAAGCCCTTG
    ATTCTGCAGC ATGAAAACCT AGAAACCATA GTTGACTTTA TAAAAAGCAC ACTACCCAAC
    CTGGGCTTGG TGCAGATGGA GAAGACTATC AGTCAGGTGT TTGAGACGGA CATCTCGAAA
    CAGTTACAAG CTTATGAAGT TGAGTACCAC GTGCTTCAGG AAGAGCTTAT CGATTCTTCT
    CCTCTCAGTG ACAACCAAAG AATGGACAAA CTAGAGAAGA CCAACAGCAG CTTACGCAAA
    CAGAACCTTG ACCTCCTTGA ACAGCTGCAG GTCGCCAATG GGAGGATCCA GAGCCTGGAA
    GCCACTGTTG AGAAGCTCCT GACTAGCGAG AGCAAGCTGA AGCAGGCGAC CCTGGCCTTG
    GAGCTGGAGA GGTCAGCCCT GCTGCAGACG GTGGAGCAGC TCCGGAGGCA GACGGCAGAG
    CTGGGCAGCC AGGAGTCTGA CCCCACACTG CCCAAGCCCT CAGGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024848801.1
    CDS163..3273
    Translation

    Target ORF information:

    RefSeq Version XM_024993033.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024993033.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGTCACCAT TGTTCAGCTG GGTGGCCAAG GTGCCTGAGA TCATCAGTTC CATCCGGCAG 
    GCTGGGAAGA TCGCCCGCCA GGAGGAGCTC CGCTGCCCTT CGGAGTTCGA TGACACATTC
    GCCAAGAAGT TCGAGGTGCT GTTCTGCGGC CGCGTGGCCG TGGCGCACAG GAAGGCTCCA
    CCCGCCCTGA TCGATGAGTG CATCGAAGGC TTCAGTCACG TGAGCGGTGG CTTCTCCTCG
    GATCAGTCCC GGTCTGCACT GCAGCCACCG GGGGATGGGG AGCGGGGCCC GCGGCCGATG
    CGCAAGTCCT TCTCGCAGCC CGGGCTGCGC TCCTTGGCTT TCCGAAAGGA GTTTCAGGAT
    GCAGGCCTGC GGAGTAGTAG TTTCTTCAGC TCTTTTGAGG AGAGCGACAT CGAGAACCAC
    CTGATTAGTG GACACAATAT TGTGCAGCCG ACAGATATTG AGGAAAATCG AACTATGCTC
    TTCACGATTG GTCAATCTGA AGTTTACCTC ATCAGTCCTG ACACCAAAAA AATAGCGTTG
    CAGAAAAATT TTAAGGAGAT ATCCTTTTGC TCTCAGGGTA TTAGACATGT GGACCACTTT
    GGGTTCATCT GCCGTGAGTC TTCCGGAGGT GGAGGCTTTC ATTTCGTCTG TTACGTGTTT
    CAGTGCACAA ATGAAGCTCT GGTGGATGAG ATCATGATGA CCTTGAAGCA GGCCTTCACG
    GTGGCTGCTG TGCAGCAGAC AGCCAAGGCG CCAGCCCAGC TGTGTGAGGG CTGCCCCCTG
    CAGGGCCTGC ACAAGCTCTG CGAGCGGATC GAGGGAATGA ATTCTTCCAA AACCAAACTA
    GAACTCCAGA AGCACCTGAC AACATTAACT AATCAGGAGC AAGCAACTAT TTTTGAGGAG
    GTTCAGAAAT TGAGACCAAG AAATGAGCAA CGAGAGAATG AATTGATTAT TTCTTTCCTG
    AGATGTTTAT ATGAAGAGAA GCAAAAAGTT CACATCCATA TTGGGGAGAT AAAACAGACG
    TCACAGATTG CAGCAGAGAA TATTGGAAGC GAATTACCAT CAAGTGCCAC TCGATTTAGG
    CTAGATATGC TGAAAAACAA AGCGAAGAGG TCTTTAACAG AATCTTTAGA AAGTATTTTG
    TCTCGGGGTA ATAAAGCCAG AGGCCTCCAG GAACACTCAG CCAGTTTGGA TCTGGACAGC
    TCCGTGTCTA GCATGTTCAG TAACACCAGC AAAGAGCCGT CTGGCTATGA AAAGGAGGCC
    TTGCCCATCT CCGAGAGCTG CTTCAGGCTC CTCGGCTCCT CGGACGACCT GTCCAGTGAC
    TCAGAGAGCC AACTCACAGA AGAGCCAGCC CTGCTCTCGC CCAAGCAGGG CTTCCGAAGG
    CGCGCCAACA CCCTGAGTCA TGTTCCCGTG GAATGCCAGG AGCCTCCACA GCTGGTGCGA
    GGGTCTCCAG GGGTCTCGCA AAGGAAACTT GTGAGGTATC ACTCAGTGAG CACAGAGACG
    CCTCATGAAC GAAAGGACTT TGAGTCCAAA GCCGACCACA TCAGCGATGC CAGCAGGACC
    CCTGTGAAGA CACGGAGACA CTCGTGGAGG CAGCAGATCT TCCTTCGGGT AGCCACCCCC
    CAGAAGGCCT GCGAGTCCCC CAAAAGATAC GAAGATTACT CAGAGTTGGG AGAGCTTCCC
    CCACGATCTC CACTAGAACC AGTTTGTGAA GATGGACCCT TTGGCCCGGT CCCAGAGGAA
    AAGAAAAGAA CATCTCATGA ACTTCGGGAG CTGTGGCAAA AGGCCATTCT ACAACAGATA
    TTGCTGCTCA GGATGGAGAA AGAAAACCAA AAACTGCAAG CCTCTGAGAA TGATTTGCTG
    AACAAGCGCC TAAAGCTTGA TTATGAAGAA ATCACTCCCT GTCTTAAAGA AGTGACTACA
    GTGTGGGAAA AGATACTTAG CACTCCAGGA AGATCAAAAA TTAAGTTTGA CATGGAAAAA
    ATGCACTCGG CTGTTGGCCA AGGTGTGCCG CGACACCACC GGGGTGAAAT CTGGAAATTC
    TTGGCGGAGC AGTACCACCT GAAGCACCCG TTTCCCTGCA AACAGCAGCC CAAGGACACG
    CCCTACAAAG AGCTCTTAAA GCAGCTCACC TCCCAGCAGC ACGCCATTCT CATCGACCTT
    GGGCGAACCT TTCCAACACA TCCGTACTAC TCTGCTCAGC TAGGAGCAGG GCAGCTGTCA
    CTTTACAACA TCCTGAAGGC CTACTCGCTT CTAGACCAAG AAGTGGGCTA TTGCCAAGGT
    CTCAGCTTTG TAGCAGGCAT CCTGCTGCTT CATATGGGTG AAGAAGAGGC GTTTAACATG
    CTCAAGTTTC TGATGTTTGA CATGGGACTG CGGAAACAAT ATCGGCCGGA CATGATTATT
    TTACAGATCC AGATGTACCA GCTCTCGAGG CTGCTCCACG ACTACCACAG GGACCTCTAC
    AACCACCTGG AGGAGCATGA GATTGGCCCC AGCCTCTATG CTGCGCCCTG GTTCCTCACC
    GTGTTTGCCT CACAGTTCCC CCTGGGCTTC GTCGCCAGAG TCTTTGATAT GATCTTTCTT
    CAGGGATCAG AGGTCATATT TAAAGTGGCT TTAAGTCTGC TGGGAAGCCA TAAGCCCTTG
    ATTCTGCAGC ATGAAAACCT AGAAACCATA GTTGACTTTA TAAAAAGCAC ACTACCCAAC
    CTGGGCTTGG TGCAGATGGA GAAGACTATC AGTCAGGTGT TTGAGACGGA CATCTCGAAA
    CAGTTACAAG CTTATGAAGT TGAGTACCAC GTGCTTCAGG AAGAGCTTAT CGATTCTTCT
    CCTCTCAGTG ACAACCAAAG AATGGACAAA CTAGAGAAGA CCAACAGCAG CTTACGCAAA
    CAGAACCTTG ACCTCCTTGA ACAGCTGCAG GTCGCCAATG GGAGGATCCA GAGCCTGGAA
    GCCACTGTTG AGAAGCTCCT GACTAGCGAG AGCAAGCTGA AGCAGGCGAC CCTGGCCTTG
    GAGCTGGAGA GGTCAGCCCT GCTGCAGACG GTGGAGCAGC TCCGGAGGCA GACGGCAGAG
    CTGGGCAGCC AGGAGTCTGA CCCCACACTG CCCAAGCCCT CAGGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20337
    Clone ID Related Accession (Same CDS sequence) XM_005207838.3 , XM_005207838.4 , NM_001166524.1
    Accession Version NM_001166524.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-23
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from NKLS02000006.1. On Oct 27, 2009 this sequence version replaced XM_611974.4. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## CDS exon combination :: SRR5571259.35129.1, SRR6764281.28651.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA AGGACTTTGA GTCCAAAGCC
    GACCACATCA GCGATGCCAG CAGGACCCCT GTGAAGACAC GGAGACACTC GTGGAGGCAG
    CAGATCTTCC TTCGGGTAGC CACCCCCCAG AAGGCCTGCG AGTCCCCCAA AAGATACGAA
    GATTACTCAG AGTTGGGAGA GCTTCCCCCA CGATCTCCAC TAGAACCAGT TTGTGAAGAT
    GGACCCTTTG GCCCGGTCCC AGAGGAAAAG AAAAGAACAT CTCATGAACT TCGGGAGCTG
    TGGCAAAAGG CCATTCTACA ACAGATATTG CTGCTCAGGA TGGAGAAAGA AAACCAAAAA
    CTGCAAGCCT CTGAGAATGA TTTGCTGAAC AAGCGCCTAA AGCTTGATTA TGAAGAAATC
    ACTCCCTGTC TTAAAGAAGT GACTACAGTG TGGGAAAAGA TACTTAGCAC TCCAGGAAGA
    TCAAAAATTA AGTTTGACAT GGAAAAAATG CACTCGGCTG TTGGCCAAGG TGTGCCGCGA
    CACCACCGGG GTGAAATCTG GAAATTCTTG GCGGAGCAGT ACCACCTGAA GCACCCGTTT
    CCCTGCAAAC AGCAGCCCAA GGACACGCCC TACAAAGAGC TCTTAAAGCA GCTCACCTCC
    CAGCAGCACG CCATTCTCAT CGACCTTGGG CGAACCTTTC CAACACATCC GTACTACTCT
    GCTCAGCTAG GAGCAGGGCA GCTGTCACTT TACAACATCC TGAAGGCCTA CTCGCTTCTA
    GACCAAGAAG TGGGCTATTG CCAAGGTCTC AGCTTTGTAG CAGGCATCCT GCTGCTTCAT
    ATGGGTGAAG AAGAGGCGTT TAACATGCTC AAGTTTCTGA TGTTTGACAT GGGACTGCGG
    AAACAATATC GGCCGGACAT GATTATTTTA CAGATCCAGA TGTACCAGCT CTCGAGGCTG
    CTCCACGACT ACCACAGGGA CCTCTACAAC CACCTGGAGG AGCATGAGAT TGGCCCCAGC
    CTCTATGCTG CGCCCTGGTT CCTCACCGTG TTTGCCTCAC AGTTCCCCCT GGGCTTCGTC
    GCCAGAGTCT TTGATATGAT CTTTCTTCAG GGATCAGAGG TCATATTTAA AGTGGCTTTA
    AGTCTGCTGG GAAGCCATAA GCCCTTGATT CTGCAGCATG AAAACCTAGA AACCATAGTT
    GACTTTATAA AAAGCACACT ACCCAACCTG GGCTTGGTGC AGATGGAGAA GACTATCAGT
    CAGGTGTTTG AGACGGACAT CTCGAAACAG TTACAAGCTT ATGAAGTTGA GTACCACGTG
    CTTCAGGAAG AGCTTATCGA TTCTTCTCCT CTCAGTGACA ACCAAAGAAT GGACAAACTA
    GAGAAGACCA ACAGCAGCTT ACGCAAACAG AACCTTGACC TCCTTGAACA GCTGCAGGTC
    GCCAATGGGA GGATCCAGAG CCTGGAAGCC ACTGTTGAGA AGCTCCTGAC TAGCGAGAGC
    AAGCTGAAGC AGGCGACCCT GGCCTTGGAG CTGGAGAGGT CAGCCCTGCT GCAGACGGTG
    GAGCAGCTCC GGAGGCAGAC GGCAGAGCTG GGCAGCCAGG AGTCTGACCC CACACTGCCC
    AAGCCCTCAG GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001159996.1
    CDS1..3498
    Translation

    Target ORF information:

    RefSeq Version NM_001166524.1
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001166524.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA AGGACTTTGA GTCCAAAGCC
    GACCACATCA GCGATGCCAG CAGGACCCCT GTGAAGACAC GGAGACACTC GTGGAGGCAG
    CAGATCTTCC TTCGGGTAGC CACCCCCCAG AAGGCCTGCG AGTCCCCCAA AAGATACGAA
    GATTACTCAG AGTTGGGAGA GCTTCCCCCA CGATCTCCAC TAGAACCAGT TTGTGAAGAT
    GGACCCTTTG GCCCGGTCCC AGAGGAAAAG AAAAGAACAT CTCATGAACT TCGGGAGCTG
    TGGCAAAAGG CCATTCTACA ACAGATATTG CTGCTCAGGA TGGAGAAAGA AAACCAAAAA
    CTGCAAGCCT CTGAGAATGA TTTGCTGAAC AAGCGCCTAA AGCTTGATTA TGAAGAAATC
    ACTCCCTGTC TTAAAGAAGT GACTACAGTG TGGGAAAAGA TACTTAGCAC TCCAGGAAGA
    TCAAAAATTA AGTTTGACAT GGAAAAAATG CACTCGGCTG TTGGCCAAGG TGTGCCGCGA
    CACCACCGGG GTGAAATCTG GAAATTCTTG GCGGAGCAGT ACCACCTGAA GCACCCGTTT
    CCCTGCAAAC AGCAGCCCAA GGACACGCCC TACAAAGAGC TCTTAAAGCA GCTCACCTCC
    CAGCAGCACG CCATTCTCAT CGACCTTGGG CGAACCTTTC CAACACATCC GTACTACTCT
    GCTCAGCTAG GAGCAGGGCA GCTGTCACTT TACAACATCC TGAAGGCCTA CTCGCTTCTA
    GACCAAGAAG TGGGCTATTG CCAAGGTCTC AGCTTTGTAG CAGGCATCCT GCTGCTTCAT
    ATGGGTGAAG AAGAGGCGTT TAACATGCTC AAGTTTCTGA TGTTTGACAT GGGACTGCGG
    AAACAATATC GGCCGGACAT GATTATTTTA CAGATCCAGA TGTACCAGCT CTCGAGGCTG
    CTCCACGACT ACCACAGGGA CCTCTACAAC CACCTGGAGG AGCATGAGAT TGGCCCCAGC
    CTCTATGCTG CGCCCTGGTT CCTCACCGTG TTTGCCTCAC AGTTCCCCCT GGGCTTCGTC
    GCCAGAGTCT TTGATATGAT CTTTCTTCAG GGATCAGAGG TCATATTTAA AGTGGCTTTA
    AGTCTGCTGG GAAGCCATAA GCCCTTGATT CTGCAGCATG AAAACCTAGA AACCATAGTT
    GACTTTATAA AAAGCACACT ACCCAACCTG GGCTTGGTGC AGATGGAGAA GACTATCAGT
    CAGGTGTTTG AGACGGACAT CTCGAAACAG TTACAAGCTT ATGAAGTTGA GTACCACGTG
    CTTCAGGAAG AGCTTATCGA TTCTTCTCCT CTCAGTGACA ACCAAAGAAT GGACAAACTA
    GAGAAGACCA ACAGCAGCTT ACGCAAACAG AACCTTGACC TCCTTGAACA GCTGCAGGTC
    GCCAATGGGA GGATCCAGAG CCTGGAAGCC ACTGTTGAGA AGCTCCTGAC TAGCGAGAGC
    AAGCTGAAGC AGGCGACCCT GGCCTTGGAG CTGGAGAGGT CAGCCCTGCT GCAGACGGTG
    GAGCAGCTCC GGAGGCAGAC GGCAGAGCTG GGCAGCCAGG AGTCTGACCC CACACTGCCC
    AAGCCCTCAG GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20334
    Clone ID Related Accession (Same CDS sequence) XM_005207835.4 , XM_005207835.3
    Accession Version XM_005207835.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGATATG
    ATCTTTCTTC AGGGATCAGA GGTCATATTT AAAGTGGCTT TAAGTCTGCT GGGAAGCCAT
    AAGCCCTTGA TTCTGCAGCA TGAAAACCTA GAAACCATAG TTGACTTTAT AAAAAGCACA
    CTACCCAACC TGGGCTTGGT GCAGATGGAG AAGACTATCA GTCAGGTGTT TGAGACGGAC
    ATCTCGAAAC AGTTACAAGC TTATGAAGTT GAGTACCACG TGCTTCAGGA AGAGCTTATC
    GATTCTTCTC CTCTCAGTGA CAACCAAAGA ATGGACAAAC TAGAGAAGAC CAACAGCAGC
    TTACGCAAAC AGAACCTTGA CCTCCTTGAA CAGCTGCAGG TCGCCAATGG GAGGATCCAG
    AGCCTGGAAG CCACTGTTGA GAAGCTCCTG ACTAGCGAGA GCAAGCTGAA GCAGGCGACC
    CTGGCCTTGG AGCTGGAGAG GTCAGCCCTG CTGCAGACGG TGGAGCAGCT CCGGAGGCAG
    ACGGCAGAGC TGGGCAGCCA GGAGTCTGAC CCCACACTGC CCAAGCCCTC AGGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207892.1
    CDS819..4598
    Translation

    Target ORF information:

    RefSeq Version XM_005207835.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207835.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGATATG
    ATCTTTCTTC AGGGATCAGA GGTCATATTT AAAGTGGCTT TAAGTCTGCT GGGAAGCCAT
    AAGCCCTTGA TTCTGCAGCA TGAAAACCTA GAAACCATAG TTGACTTTAT AAAAAGCACA
    CTACCCAACC TGGGCTTGGT GCAGATGGAG AAGACTATCA GTCAGGTGTT TGAGACGGAC
    ATCTCGAAAC AGTTACAAGC TTATGAAGTT GAGTACCACG TGCTTCAGGA AGAGCTTATC
    GATTCTTCTC CTCTCAGTGA CAACCAAAGA ATGGACAAAC TAGAGAAGAC CAACAGCAGC
    TTACGCAAAC AGAACCTTGA CCTCCTTGAA CAGCTGCAGG TCGCCAATGG GAGGATCCAG
    AGCCTGGAAG CCACTGTTGA GAAGCTCCTG ACTAGCGAGA GCAAGCTGAA GCAGGCGACC
    CTGGCCTTGG AGCTGGAGAG GTCAGCCCTG CTGCAGACGG TGGAGCAGCT CCGGAGGCAG
    ACGGCAGAGC TGGGCAGCCA GGAGTCTGAC CCCACACTGC CCAAGCCCTC AGGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20334
    Clone ID Related Accession (Same CDS sequence) XM_005207835.4 , XM_005207835.3
    Accession Version XM_005207835.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3780bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207835.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGATATG
    ATCTTTCTTC AGGGATCAGA GGTCATATTT AAAGTGGCTT TAAGTCTGCT GGGAAGCCAT
    AAGCCCTTGA TTCTGCAGCA TGAAAACCTA GAAACCATAG TTGACTTTAT AAAAAGCACA
    CTACCCAACC TGGGCTTGGT GCAGATGGAG AAGACTATCA GTCAGGTGTT TGAGACGGAC
    ATCTCGAAAC AGTTACAAGC TTATGAAGTT GAGTACCACG TGCTTCAGGA AGAGCTTATC
    GATTCTTCTC CTCTCAGTGA CAACCAAAGA ATGGACAAAC TAGAGAAGAC CAACAGCAGC
    TTACGCAAAC AGAACCTTGA CCTCCTTGAA CAGCTGCAGG TCGCCAATGG GAGGATCCAG
    AGCCTGGAAG CCACTGTTGA GAAGCTCCTG ACTAGCGAGA GCAAGCTGAA GCAGGCGACC
    CTGGCCTTGG AGCTGGAGAG GTCAGCCCTG CTGCAGACGG TGGAGCAGCT CCGGAGGCAG
    ACGGCAGAGC TGGGCAGCCA GGAGTCTGAC CCCACACTGC CCAAGCCCTC AGGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207892.1
    CDS187..3966
    Translation

    Target ORF information:

    RefSeq Version XM_005207835.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207835.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGATATG
    ATCTTTCTTC AGGGATCAGA GGTCATATTT AAAGTGGCTT TAAGTCTGCT GGGAAGCCAT
    AAGCCCTTGA TTCTGCAGCA TGAAAACCTA GAAACCATAG TTGACTTTAT AAAAAGCACA
    CTACCCAACC TGGGCTTGGT GCAGATGGAG AAGACTATCA GTCAGGTGTT TGAGACGGAC
    ATCTCGAAAC AGTTACAAGC TTATGAAGTT GAGTACCACG TGCTTCAGGA AGAGCTTATC
    GATTCTTCTC CTCTCAGTGA CAACCAAAGA ATGGACAAAC TAGAGAAGAC CAACAGCAGC
    TTACGCAAAC AGAACCTTGA CCTCCTTGAA CAGCTGCAGG TCGCCAATGG GAGGATCCAG
    AGCCTGGAAG CCACTGTTGA GAAGCTCCTG ACTAGCGAGA GCAAGCTGAA GCAGGCGACC
    CTGGCCTTGG AGCTGGAGAG GTCAGCCCTG CTGCAGACGG TGGAGCAGCT CCGGAGGCAG
    ACGGCAGAGC TGGGCAGCCA GGAGTCTGAC CCCACACTGC CCAAGCCCTC AGGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20335
    Clone ID Related Accession (Same CDS sequence) XM_005207836.4 , XM_005207836.3
    Accession Version XM_005207836.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207836.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGTGGGAATT GTCCACCTGT TGGCGAGTCT CAAAGTCCCT CAGGTCAGTC TTCAGCTCCT
    GCTCCTCCAC CTCGTCTTAA CCCCTCCGCC TCCTCGCCTA ATTTTTTTAA GTACCTCAAA
    CATAACCCGA GTGGAGAACA GAGTGGGAAT GCCGTGCCAA AGAGCATCTC CTACCGTAAT
    GCCCTGCGGA AAAAACTTCA TTCCTCTTCC TCTGTGCCAA ATTTTCTAAA ATTTCTGGCT
    CCTGTAGATG AAAATAACAC CTCTGACTTT ATGAACACTC AAAGGGACTT TGAGTCCAAA
    GCCGACCACA TCAGCGATGC CAGCAGGACC CCTGTGAAGA CACGGAGACA CTCGTGGAGG
    CAGCAGATCT TCCTTCGGGT AGCCACCCCC CAGAAGGCCT GCGAGTCCCC CAAAAGATAC
    GAAGATTACT CAGAGTTGGG AGAGCTTCCC CCACGATCTC CACTAGAACC AGTTTGTGAA
    GATGGACCCT TTGGCCCGGT CCCAGAGGAA AAGAAAAGAA CATCTCATGA ACTTCGGGAG
    CTGTGGCAAA AGGCCATTCT ACAACAGATA TTGCTGCTCA GGATGGAGAA AGAAAACCAA
    AAACTGCAAG CCTCTGAGAA TGATTTGCTG AACAAGCGCC TAAAGCTTGA TTATGAAGAA
    ATCACTCCCT GTCTTAAAGA AGTGACTACA GTGTGGGAAA AGATACTTAG CACTCCAGGA
    AGATCAAAAA TTAAGTTTGA CATGGAAAAA ATGCACTCGG CTGTTGGCCA AGGTGTGCCG
    CGACACCACC GGGGTGAAAT CTGGAAATTC TTGGCGGAGC AGTACCACCT GAAGCACCCG
    TTTCCCTGCA AACAGCAGCC CAAGGACACG CCCTACAAAG AGCTCTTAAA GCAGCTCACC
    TCCCAGCAGC ACGCCATTCT CATCGACCTT GGGCGAACCT TTCCAACACA TCCGTACTAC
    TCTGCTCAGC TAGGAGCAGG GCAGCTGTCA CTTTACAACA TCCTGAAGGC CTACTCGCTT
    CTAGACCAAG AAGTGGGCTA TTGCCAAGGT CTCAGCTTTG TAGCAGGCAT CCTGCTGCTT
    CATATGGGTG AAGAAGAGGC GTTTAACATG CTCAAGTTTC TGATGTTTGA CATGGGACTG
    CGGAAACAAT ATCGGCCGGA CATGATTATT TTACAGATCC AGATGTACCA GCTCTCGAGG
    CTGCTCCACG ACTACCACAG GGACCTCTAC AACCACCTGG AGGAGCATGA GATTGGCCCC
    AGCCTCTATG CTGCGCCCTG GTTCCTCACC GTGTTTGCCT CACAGTTCCC CCTGGGCTTC
    GTCGCCAGAG TCTTTGATAT GATCTTTCTT CAGGGATCAG AGGTCATATT TAAAGTGGCT
    TTAAGTCTGC TGGGAAGCCA TAAGCCCTTG ATTCTGCAGC ATGAAAACCT AGAAACCATA
    GTTGACTTTA TAAAAAGCAC ACTACCCAAC CTGGGCTTGG TGCAGATGGA GAAGACTATC
    AGTCAGGTGT TTGAGACGGA CATCTCGAAA CAGTTACAAG CTTATGAAGT TGAGTACCAC
    GTGCTTCAGG AAGAGCTTAT CGATTCTTCT CCTCTCAGTG ACAACCAAAG AATGGACAAA
    CTAGAGAAGA CCAACAGCAG CTTACGCAAA CAGAACCTTG ACCTCCTTGA ACAGCTGCAG
    GTCGCCAATG GGAGGATCCA GAGCCTGGAA GCCACTGTTG AGAAGCTCCT GACTAGCGAG
    AGCAAGCTGA AGCAGGCGAC CCTGGCCTTG GAGCTGGAGA GGTCAGCCCT GCTGCAGACG
    GTGGAGCAGC TCCGGAGGCA GACGGCAGAG CTGGGCAGCC AGGAGTCTGA CCCCACACTG
    CCCAAGCCCT CAGGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207893.1
    CDS333..4073
    Translation

    Target ORF information:

    RefSeq Version XM_005207836.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207836.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGTGGGAATT GTCCACCTGT TGGCGAGTCT CAAAGTCCCT CAGGTCAGTC TTCAGCTCCT
    GCTCCTCCAC CTCGTCTTAA CCCCTCCGCC TCCTCGCCTA ATTTTTTTAA GTACCTCAAA
    CATAACCCGA GTGGAGAACA GAGTGGGAAT GCCGTGCCAA AGAGCATCTC CTACCGTAAT
    GCCCTGCGGA AAAAACTTCA TTCCTCTTCC TCTGTGCCAA ATTTTCTAAA ATTTCTGGCT
    CCTGTAGATG AAAATAACAC CTCTGACTTT ATGAACACTC AAAGGGACTT TGAGTCCAAA
    GCCGACCACA TCAGCGATGC CAGCAGGACC CCTGTGAAGA CACGGAGACA CTCGTGGAGG
    CAGCAGATCT TCCTTCGGGT AGCCACCCCC CAGAAGGCCT GCGAGTCCCC CAAAAGATAC
    GAAGATTACT CAGAGTTGGG AGAGCTTCCC CCACGATCTC CACTAGAACC AGTTTGTGAA
    GATGGACCCT TTGGCCCGGT CCCAGAGGAA AAGAAAAGAA CATCTCATGA ACTTCGGGAG
    CTGTGGCAAA AGGCCATTCT ACAACAGATA TTGCTGCTCA GGATGGAGAA AGAAAACCAA
    AAACTGCAAG CCTCTGAGAA TGATTTGCTG AACAAGCGCC TAAAGCTTGA TTATGAAGAA
    ATCACTCCCT GTCTTAAAGA AGTGACTACA GTGTGGGAAA AGATACTTAG CACTCCAGGA
    AGATCAAAAA TTAAGTTTGA CATGGAAAAA ATGCACTCGG CTGTTGGCCA AGGTGTGCCG
    CGACACCACC GGGGTGAAAT CTGGAAATTC TTGGCGGAGC AGTACCACCT GAAGCACCCG
    TTTCCCTGCA AACAGCAGCC CAAGGACACG CCCTACAAAG AGCTCTTAAA GCAGCTCACC
    TCCCAGCAGC ACGCCATTCT CATCGACCTT GGGCGAACCT TTCCAACACA TCCGTACTAC
    TCTGCTCAGC TAGGAGCAGG GCAGCTGTCA CTTTACAACA TCCTGAAGGC CTACTCGCTT
    CTAGACCAAG AAGTGGGCTA TTGCCAAGGT CTCAGCTTTG TAGCAGGCAT CCTGCTGCTT
    CATATGGGTG AAGAAGAGGC GTTTAACATG CTCAAGTTTC TGATGTTTGA CATGGGACTG
    CGGAAACAAT ATCGGCCGGA CATGATTATT TTACAGATCC AGATGTACCA GCTCTCGAGG
    CTGCTCCACG ACTACCACAG GGACCTCTAC AACCACCTGG AGGAGCATGA GATTGGCCCC
    AGCCTCTATG CTGCGCCCTG GTTCCTCACC GTGTTTGCCT CACAGTTCCC CCTGGGCTTC
    GTCGCCAGAG TCTTTGATAT GATCTTTCTT CAGGGATCAG AGGTCATATT TAAAGTGGCT
    TTAAGTCTGC TGGGAAGCCA TAAGCCCTTG ATTCTGCAGC ATGAAAACCT AGAAACCATA
    GTTGACTTTA TAAAAAGCAC ACTACCCAAC CTGGGCTTGG TGCAGATGGA GAAGACTATC
    AGTCAGGTGT TTGAGACGGA CATCTCGAAA CAGTTACAAG CTTATGAAGT TGAGTACCAC
    GTGCTTCAGG AAGAGCTTAT CGATTCTTCT CCTCTCAGTG ACAACCAAAG AATGGACAAA
    CTAGAGAAGA CCAACAGCAG CTTACGCAAA CAGAACCTTG ACCTCCTTGA ACAGCTGCAG
    GTCGCCAATG GGAGGATCCA GAGCCTGGAA GCCACTGTTG AGAAGCTCCT GACTAGCGAG
    AGCAAGCTGA AGCAGGCGAC CCTGGCCTTG GAGCTGGAGA GGTCAGCCCT GCTGCAGACG
    GTGGAGCAGC TCCGGAGGCA GACGGCAGAG CTGGGCAGCC AGGAGTCTGA CCCCACACTG
    CCCAAGCCCT CAGGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20335
    Clone ID Related Accession (Same CDS sequence) XM_005207836.4 , XM_005207836.3
    Accession Version XM_005207836.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207836.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGTGGGAATT GTCCACCTGT TGGCGAGTCT CAAAGTCCCT CAGGTCAGTC TTCAGCTCCT
    GCTCCTCCAC CTCGTCTTAA CCCCTCCGCC TCCTCGCCTA ATTTTTTTAA GTACCTCAAA
    CATAACCCGA GTGGAGAACA GAGTGGGAAT GCCGTGCCAA AGAGCATCTC CTACCGTAAT
    GCCCTGCGGA AAAAACTTCA TTCCTCTTCC TCTGTGCCAA ATTTTCTAAA ATTTCTGGCT
    CCTGTAGATG AAAATAACAC CTCTGACTTT ATGAACACTC AAAGGGACTT TGAGTCCAAA
    GCCGACCACA TCAGCGATGC CAGCAGGACC CCTGTGAAGA CACGGAGACA CTCGTGGAGG
    CAGCAGATCT TCCTTCGGGT AGCCACCCCC CAGAAGGCCT GCGAGTCCCC CAAAAGATAC
    GAAGATTACT CAGAGTTGGG AGAGCTTCCC CCACGATCTC CACTAGAACC AGTTTGTGAA
    GATGGACCCT TTGGCCCGGT CCCAGAGGAA AAGAAAAGAA CATCTCATGA ACTTCGGGAG
    CTGTGGCAAA AGGCCATTCT ACAACAGATA TTGCTGCTCA GGATGGAGAA AGAAAACCAA
    AAACTGCAAG CCTCTGAGAA TGATTTGCTG AACAAGCGCC TAAAGCTTGA TTATGAAGAA
    ATCACTCCCT GTCTTAAAGA AGTGACTACA GTGTGGGAAA AGATACTTAG CACTCCAGGA
    AGATCAAAAA TTAAGTTTGA CATGGAAAAA ATGCACTCGG CTGTTGGCCA AGGTGTGCCG
    CGACACCACC GGGGTGAAAT CTGGAAATTC TTGGCGGAGC AGTACCACCT GAAGCACCCG
    TTTCCCTGCA AACAGCAGCC CAAGGACACG CCCTACAAAG AGCTCTTAAA GCAGCTCACC
    TCCCAGCAGC ACGCCATTCT CATCGACCTT GGGCGAACCT TTCCAACACA TCCGTACTAC
    TCTGCTCAGC TAGGAGCAGG GCAGCTGTCA CTTTACAACA TCCTGAAGGC CTACTCGCTT
    CTAGACCAAG AAGTGGGCTA TTGCCAAGGT CTCAGCTTTG TAGCAGGCAT CCTGCTGCTT
    CATATGGGTG AAGAAGAGGC GTTTAACATG CTCAAGTTTC TGATGTTTGA CATGGGACTG
    CGGAAACAAT ATCGGCCGGA CATGATTATT TTACAGATCC AGATGTACCA GCTCTCGAGG
    CTGCTCCACG ACTACCACAG GGACCTCTAC AACCACCTGG AGGAGCATGA GATTGGCCCC
    AGCCTCTATG CTGCGCCCTG GTTCCTCACC GTGTTTGCCT CACAGTTCCC CCTGGGCTTC
    GTCGCCAGAG TCTTTGATAT GATCTTTCTT CAGGGATCAG AGGTCATATT TAAAGTGGCT
    TTAAGTCTGC TGGGAAGCCA TAAGCCCTTG ATTCTGCAGC ATGAAAACCT AGAAACCATA
    GTTGACTTTA TAAAAAGCAC ACTACCCAAC CTGGGCTTGG TGCAGATGGA GAAGACTATC
    AGTCAGGTGT TTGAGACGGA CATCTCGAAA CAGTTACAAG CTTATGAAGT TGAGTACCAC
    GTGCTTCAGG AAGAGCTTAT CGATTCTTCT CCTCTCAGTG ACAACCAAAG AATGGACAAA
    CTAGAGAAGA CCAACAGCAG CTTACGCAAA CAGAACCTTG ACCTCCTTGA ACAGCTGCAG
    GTCGCCAATG GGAGGATCCA GAGCCTGGAA GCCACTGTTG AGAAGCTCCT GACTAGCGAG
    AGCAAGCTGA AGCAGGCGAC CCTGGCCTTG GAGCTGGAGA GGTCAGCCCT GCTGCAGACG
    GTGGAGCAGC TCCGGAGGCA GACGGCAGAG CTGGGCAGCC AGGAGTCTGA CCCCACACTG
    CCCAAGCCCT CAGGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207893.1
    CDS187..3927
    Translation

    Target ORF information:

    RefSeq Version XM_005207836.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207836.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGTGGGAATT GTCCACCTGT TGGCGAGTCT CAAAGTCCCT CAGGTCAGTC TTCAGCTCCT
    GCTCCTCCAC CTCGTCTTAA CCCCTCCGCC TCCTCGCCTA ATTTTTTTAA GTACCTCAAA
    CATAACCCGA GTGGAGAACA GAGTGGGAAT GCCGTGCCAA AGAGCATCTC CTACCGTAAT
    GCCCTGCGGA AAAAACTTCA TTCCTCTTCC TCTGTGCCAA ATTTTCTAAA ATTTCTGGCT
    CCTGTAGATG AAAATAACAC CTCTGACTTT ATGAACACTC AAAGGGACTT TGAGTCCAAA
    GCCGACCACA TCAGCGATGC CAGCAGGACC CCTGTGAAGA CACGGAGACA CTCGTGGAGG
    CAGCAGATCT TCCTTCGGGT AGCCACCCCC CAGAAGGCCT GCGAGTCCCC CAAAAGATAC
    GAAGATTACT CAGAGTTGGG AGAGCTTCCC CCACGATCTC CACTAGAACC AGTTTGTGAA
    GATGGACCCT TTGGCCCGGT CCCAGAGGAA AAGAAAAGAA CATCTCATGA ACTTCGGGAG
    CTGTGGCAAA AGGCCATTCT ACAACAGATA TTGCTGCTCA GGATGGAGAA AGAAAACCAA
    AAACTGCAAG CCTCTGAGAA TGATTTGCTG AACAAGCGCC TAAAGCTTGA TTATGAAGAA
    ATCACTCCCT GTCTTAAAGA AGTGACTACA GTGTGGGAAA AGATACTTAG CACTCCAGGA
    AGATCAAAAA TTAAGTTTGA CATGGAAAAA ATGCACTCGG CTGTTGGCCA AGGTGTGCCG
    CGACACCACC GGGGTGAAAT CTGGAAATTC TTGGCGGAGC AGTACCACCT GAAGCACCCG
    TTTCCCTGCA AACAGCAGCC CAAGGACACG CCCTACAAAG AGCTCTTAAA GCAGCTCACC
    TCCCAGCAGC ACGCCATTCT CATCGACCTT GGGCGAACCT TTCCAACACA TCCGTACTAC
    TCTGCTCAGC TAGGAGCAGG GCAGCTGTCA CTTTACAACA TCCTGAAGGC CTACTCGCTT
    CTAGACCAAG AAGTGGGCTA TTGCCAAGGT CTCAGCTTTG TAGCAGGCAT CCTGCTGCTT
    CATATGGGTG AAGAAGAGGC GTTTAACATG CTCAAGTTTC TGATGTTTGA CATGGGACTG
    CGGAAACAAT ATCGGCCGGA CATGATTATT TTACAGATCC AGATGTACCA GCTCTCGAGG
    CTGCTCCACG ACTACCACAG GGACCTCTAC AACCACCTGG AGGAGCATGA GATTGGCCCC
    AGCCTCTATG CTGCGCCCTG GTTCCTCACC GTGTTTGCCT CACAGTTCCC CCTGGGCTTC
    GTCGCCAGAG TCTTTGATAT GATCTTTCTT CAGGGATCAG AGGTCATATT TAAAGTGGCT
    TTAAGTCTGC TGGGAAGCCA TAAGCCCTTG ATTCTGCAGC ATGAAAACCT AGAAACCATA
    GTTGACTTTA TAAAAAGCAC ACTACCCAAC CTGGGCTTGG TGCAGATGGA GAAGACTATC
    AGTCAGGTGT TTGAGACGGA CATCTCGAAA CAGTTACAAG CTTATGAAGT TGAGTACCAC
    GTGCTTCAGG AAGAGCTTAT CGATTCTTCT CCTCTCAGTG ACAACCAAAG AATGGACAAA
    CTAGAGAAGA CCAACAGCAG CTTACGCAAA CAGAACCTTG ACCTCCTTGA ACAGCTGCAG
    GTCGCCAATG GGAGGATCCA GAGCCTGGAA GCCACTGTTG AGAAGCTCCT GACTAGCGAG
    AGCAAGCTGA AGCAGGCGAC CCTGGCCTTG GAGCTGGAGA GGTCAGCCCT GCTGCAGACG
    GTGGAGCAGC TCCGGAGGCA GACGGCAGAG CTGGGCAGCC AGGAGTCTGA CCCCACACTG
    CCCAAGCCCT CAGGTGACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20337
    Clone ID Related Accession (Same CDS sequence) XM_005207838.3 , XM_005207838.4 , NM_001166524.1
    Accession Version XM_005207838.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207838.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA AGGACTTTGA GTCCAAAGCC
    GACCACATCA GCGATGCCAG CAGGACCCCT GTGAAGACAC GGAGACACTC GTGGAGGCAG
    CAGATCTTCC TTCGGGTAGC CACCCCCCAG AAGGCCTGCG AGTCCCCCAA AAGATACGAA
    GATTACTCAG AGTTGGGAGA GCTTCCCCCA CGATCTCCAC TAGAACCAGT TTGTGAAGAT
    GGACCCTTTG GCCCGGTCCC AGAGGAAAAG AAAAGAACAT CTCATGAACT TCGGGAGCTG
    TGGCAAAAGG CCATTCTACA ACAGATATTG CTGCTCAGGA TGGAGAAAGA AAACCAAAAA
    CTGCAAGCCT CTGAGAATGA TTTGCTGAAC AAGCGCCTAA AGCTTGATTA TGAAGAAATC
    ACTCCCTGTC TTAAAGAAGT GACTACAGTG TGGGAAAAGA TACTTAGCAC TCCAGGAAGA
    TCAAAAATTA AGTTTGACAT GGAAAAAATG CACTCGGCTG TTGGCCAAGG TGTGCCGCGA
    CACCACCGGG GTGAAATCTG GAAATTCTTG GCGGAGCAGT ACCACCTGAA GCACCCGTTT
    CCCTGCAAAC AGCAGCCCAA GGACACGCCC TACAAAGAGC TCTTAAAGCA GCTCACCTCC
    CAGCAGCACG CCATTCTCAT CGACCTTGGG CGAACCTTTC CAACACATCC GTACTACTCT
    GCTCAGCTAG GAGCAGGGCA GCTGTCACTT TACAACATCC TGAAGGCCTA CTCGCTTCTA
    GACCAAGAAG TGGGCTATTG CCAAGGTCTC AGCTTTGTAG CAGGCATCCT GCTGCTTCAT
    ATGGGTGAAG AAGAGGCGTT TAACATGCTC AAGTTTCTGA TGTTTGACAT GGGACTGCGG
    AAACAATATC GGCCGGACAT GATTATTTTA CAGATCCAGA TGTACCAGCT CTCGAGGCTG
    CTCCACGACT ACCACAGGGA CCTCTACAAC CACCTGGAGG AGCATGAGAT TGGCCCCAGC
    CTCTATGCTG CGCCCTGGTT CCTCACCGTG TTTGCCTCAC AGTTCCCCCT GGGCTTCGTC
    GCCAGAGTCT TTGATATGAT CTTTCTTCAG GGATCAGAGG TCATATTTAA AGTGGCTTTA
    AGTCTGCTGG GAAGCCATAA GCCCTTGATT CTGCAGCATG AAAACCTAGA AACCATAGTT
    GACTTTATAA AAAGCACACT ACCCAACCTG GGCTTGGTGC AGATGGAGAA GACTATCAGT
    CAGGTGTTTG AGACGGACAT CTCGAAACAG TTACAAGCTT ATGAAGTTGA GTACCACGTG
    CTTCAGGAAG AGCTTATCGA TTCTTCTCCT CTCAGTGACA ACCAAAGAAT GGACAAACTA
    GAGAAGACCA ACAGCAGCTT ACGCAAACAG AACCTTGACC TCCTTGAACA GCTGCAGGTC
    GCCAATGGGA GGATCCAGAG CCTGGAAGCC ACTGTTGAGA AGCTCCTGAC TAGCGAGAGC
    AAGCTGAAGC AGGCGACCCT GGCCTTGGAG CTGGAGAGGT CAGCCCTGCT GCAGACGGTG
    GAGCAGCTCC GGAGGCAGAC GGCAGAGCTG GGCAGCCAGG AGTCTGACCC CACACTGCCC
    AAGCCCTCAG GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207895.1
    CDS187..3684
    Translation

    Target ORF information:

    RefSeq Version XM_005207838.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207838.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA AGGACTTTGA GTCCAAAGCC
    GACCACATCA GCGATGCCAG CAGGACCCCT GTGAAGACAC GGAGACACTC GTGGAGGCAG
    CAGATCTTCC TTCGGGTAGC CACCCCCCAG AAGGCCTGCG AGTCCCCCAA AAGATACGAA
    GATTACTCAG AGTTGGGAGA GCTTCCCCCA CGATCTCCAC TAGAACCAGT TTGTGAAGAT
    GGACCCTTTG GCCCGGTCCC AGAGGAAAAG AAAAGAACAT CTCATGAACT TCGGGAGCTG
    TGGCAAAAGG CCATTCTACA ACAGATATTG CTGCTCAGGA TGGAGAAAGA AAACCAAAAA
    CTGCAAGCCT CTGAGAATGA TTTGCTGAAC AAGCGCCTAA AGCTTGATTA TGAAGAAATC
    ACTCCCTGTC TTAAAGAAGT GACTACAGTG TGGGAAAAGA TACTTAGCAC TCCAGGAAGA
    TCAAAAATTA AGTTTGACAT GGAAAAAATG CACTCGGCTG TTGGCCAAGG TGTGCCGCGA
    CACCACCGGG GTGAAATCTG GAAATTCTTG GCGGAGCAGT ACCACCTGAA GCACCCGTTT
    CCCTGCAAAC AGCAGCCCAA GGACACGCCC TACAAAGAGC TCTTAAAGCA GCTCACCTCC
    CAGCAGCACG CCATTCTCAT CGACCTTGGG CGAACCTTTC CAACACATCC GTACTACTCT
    GCTCAGCTAG GAGCAGGGCA GCTGTCACTT TACAACATCC TGAAGGCCTA CTCGCTTCTA
    GACCAAGAAG TGGGCTATTG CCAAGGTCTC AGCTTTGTAG CAGGCATCCT GCTGCTTCAT
    ATGGGTGAAG AAGAGGCGTT TAACATGCTC AAGTTTCTGA TGTTTGACAT GGGACTGCGG
    AAACAATATC GGCCGGACAT GATTATTTTA CAGATCCAGA TGTACCAGCT CTCGAGGCTG
    CTCCACGACT ACCACAGGGA CCTCTACAAC CACCTGGAGG AGCATGAGAT TGGCCCCAGC
    CTCTATGCTG CGCCCTGGTT CCTCACCGTG TTTGCCTCAC AGTTCCCCCT GGGCTTCGTC
    GCCAGAGTCT TTGATATGAT CTTTCTTCAG GGATCAGAGG TCATATTTAA AGTGGCTTTA
    AGTCTGCTGG GAAGCCATAA GCCCTTGATT CTGCAGCATG AAAACCTAGA AACCATAGTT
    GACTTTATAA AAAGCACACT ACCCAACCTG GGCTTGGTGC AGATGGAGAA GACTATCAGT
    CAGGTGTTTG AGACGGACAT CTCGAAACAG TTACAAGCTT ATGAAGTTGA GTACCACGTG
    CTTCAGGAAG AGCTTATCGA TTCTTCTCCT CTCAGTGACA ACCAAAGAAT GGACAAACTA
    GAGAAGACCA ACAGCAGCTT ACGCAAACAG AACCTTGACC TCCTTGAACA GCTGCAGGTC
    GCCAATGGGA GGATCCAGAG CCTGGAAGCC ACTGTTGAGA AGCTCCTGAC TAGCGAGAGC
    AAGCTGAAGC AGGCGACCCT GGCCTTGGAG CTGGAGAGGT CAGCCCTGCT GCAGACGGTG
    GAGCAGCTCC GGAGGCAGAC GGCAGAGCTG GGCAGCCAGG AGTCTGACCC CACACTGCCC
    AAGCCCTCAG GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20337
    Clone ID Related Accession (Same CDS sequence) XM_005207838.3 , XM_005207838.4 , NM_001166524.1
    Accession Version XM_005207838.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3498bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207838.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA AGGACTTTGA GTCCAAAGCC
    GACCACATCA GCGATGCCAG CAGGACCCCT GTGAAGACAC GGAGACACTC GTGGAGGCAG
    CAGATCTTCC TTCGGGTAGC CACCCCCCAG AAGGCCTGCG AGTCCCCCAA AAGATACGAA
    GATTACTCAG AGTTGGGAGA GCTTCCCCCA CGATCTCCAC TAGAACCAGT TTGTGAAGAT
    GGACCCTTTG GCCCGGTCCC AGAGGAAAAG AAAAGAACAT CTCATGAACT TCGGGAGCTG
    TGGCAAAAGG CCATTCTACA ACAGATATTG CTGCTCAGGA TGGAGAAAGA AAACCAAAAA
    CTGCAAGCCT CTGAGAATGA TTTGCTGAAC AAGCGCCTAA AGCTTGATTA TGAAGAAATC
    ACTCCCTGTC TTAAAGAAGT GACTACAGTG TGGGAAAAGA TACTTAGCAC TCCAGGAAGA
    TCAAAAATTA AGTTTGACAT GGAAAAAATG CACTCGGCTG TTGGCCAAGG TGTGCCGCGA
    CACCACCGGG GTGAAATCTG GAAATTCTTG GCGGAGCAGT ACCACCTGAA GCACCCGTTT
    CCCTGCAAAC AGCAGCCCAA GGACACGCCC TACAAAGAGC TCTTAAAGCA GCTCACCTCC
    CAGCAGCACG CCATTCTCAT CGACCTTGGG CGAACCTTTC CAACACATCC GTACTACTCT
    GCTCAGCTAG GAGCAGGGCA GCTGTCACTT TACAACATCC TGAAGGCCTA CTCGCTTCTA
    GACCAAGAAG TGGGCTATTG CCAAGGTCTC AGCTTTGTAG CAGGCATCCT GCTGCTTCAT
    ATGGGTGAAG AAGAGGCGTT TAACATGCTC AAGTTTCTGA TGTTTGACAT GGGACTGCGG
    AAACAATATC GGCCGGACAT GATTATTTTA CAGATCCAGA TGTACCAGCT CTCGAGGCTG
    CTCCACGACT ACCACAGGGA CCTCTACAAC CACCTGGAGG AGCATGAGAT TGGCCCCAGC
    CTCTATGCTG CGCCCTGGTT CCTCACCGTG TTTGCCTCAC AGTTCCCCCT GGGCTTCGTC
    GCCAGAGTCT TTGATATGAT CTTTCTTCAG GGATCAGAGG TCATATTTAA AGTGGCTTTA
    AGTCTGCTGG GAAGCCATAA GCCCTTGATT CTGCAGCATG AAAACCTAGA AACCATAGTT
    GACTTTATAA AAAGCACACT ACCCAACCTG GGCTTGGTGC AGATGGAGAA GACTATCAGT
    CAGGTGTTTG AGACGGACAT CTCGAAACAG TTACAAGCTT ATGAAGTTGA GTACCACGTG
    CTTCAGGAAG AGCTTATCGA TTCTTCTCCT CTCAGTGACA ACCAAAGAAT GGACAAACTA
    GAGAAGACCA ACAGCAGCTT ACGCAAACAG AACCTTGACC TCCTTGAACA GCTGCAGGTC
    GCCAATGGGA GGATCCAGAG CCTGGAAGCC ACTGTTGAGA AGCTCCTGAC TAGCGAGAGC
    AAGCTGAAGC AGGCGACCCT GGCCTTGGAG CTGGAGAGGT CAGCCCTGCT GCAGACGGTG
    GAGCAGCTCC GGAGGCAGAC GGCAGAGCTG GGCAGCCAGG AGTCTGACCC CACACTGCCC
    AAGCCCTCAG GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207895.1
    CDS323..3820
    Translation

    Target ORF information:

    RefSeq Version XM_005207838.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207838.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA AGGACTTTGA GTCCAAAGCC
    GACCACATCA GCGATGCCAG CAGGACCCCT GTGAAGACAC GGAGACACTC GTGGAGGCAG
    CAGATCTTCC TTCGGGTAGC CACCCCCCAG AAGGCCTGCG AGTCCCCCAA AAGATACGAA
    GATTACTCAG AGTTGGGAGA GCTTCCCCCA CGATCTCCAC TAGAACCAGT TTGTGAAGAT
    GGACCCTTTG GCCCGGTCCC AGAGGAAAAG AAAAGAACAT CTCATGAACT TCGGGAGCTG
    TGGCAAAAGG CCATTCTACA ACAGATATTG CTGCTCAGGA TGGAGAAAGA AAACCAAAAA
    CTGCAAGCCT CTGAGAATGA TTTGCTGAAC AAGCGCCTAA AGCTTGATTA TGAAGAAATC
    ACTCCCTGTC TTAAAGAAGT GACTACAGTG TGGGAAAAGA TACTTAGCAC TCCAGGAAGA
    TCAAAAATTA AGTTTGACAT GGAAAAAATG CACTCGGCTG TTGGCCAAGG TGTGCCGCGA
    CACCACCGGG GTGAAATCTG GAAATTCTTG GCGGAGCAGT ACCACCTGAA GCACCCGTTT
    CCCTGCAAAC AGCAGCCCAA GGACACGCCC TACAAAGAGC TCTTAAAGCA GCTCACCTCC
    CAGCAGCACG CCATTCTCAT CGACCTTGGG CGAACCTTTC CAACACATCC GTACTACTCT
    GCTCAGCTAG GAGCAGGGCA GCTGTCACTT TACAACATCC TGAAGGCCTA CTCGCTTCTA
    GACCAAGAAG TGGGCTATTG CCAAGGTCTC AGCTTTGTAG CAGGCATCCT GCTGCTTCAT
    ATGGGTGAAG AAGAGGCGTT TAACATGCTC AAGTTTCTGA TGTTTGACAT GGGACTGCGG
    AAACAATATC GGCCGGACAT GATTATTTTA CAGATCCAGA TGTACCAGCT CTCGAGGCTG
    CTCCACGACT ACCACAGGGA CCTCTACAAC CACCTGGAGG AGCATGAGAT TGGCCCCAGC
    CTCTATGCTG CGCCCTGGTT CCTCACCGTG TTTGCCTCAC AGTTCCCCCT GGGCTTCGTC
    GCCAGAGTCT TTGATATGAT CTTTCTTCAG GGATCAGAGG TCATATTTAA AGTGGCTTTA
    AGTCTGCTGG GAAGCCATAA GCCCTTGATT CTGCAGCATG AAAACCTAGA AACCATAGTT
    GACTTTATAA AAAGCACACT ACCCAACCTG GGCTTGGTGC AGATGGAGAA GACTATCAGT
    CAGGTGTTTG AGACGGACAT CTCGAAACAG TTACAAGCTT ATGAAGTTGA GTACCACGTG
    CTTCAGGAAG AGCTTATCGA TTCTTCTCCT CTCAGTGACA ACCAAAGAAT GGACAAACTA
    GAGAAGACCA ACAGCAGCTT ACGCAAACAG AACCTTGACC TCCTTGAACA GCTGCAGGTC
    GCCAATGGGA GGATCCAGAG CCTGGAAGCC ACTGTTGAGA AGCTCCTGAC TAGCGAGAGC
    AAGCTGAAGC AGGCGACCCT GGCCTTGGAG CTGGAGAGGT CAGCCCTGCT GCAGACGGTG
    GAGCAGCTCC GGAGGCAGAC GGCAGAGCTG GGCAGCCAGG AGTCTGACCC CACACTGCCC
    AAGCCCTCAG GTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20338
    Clone ID Related Accession (Same CDS sequence) XM_005207839.4 , XM_005207839.3
    Accession Version XM_005207839.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207839.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGGACTTTG AGTCCAAAGC CGACCACATC AGCGATGCCA GCAGGACCCC TGTGAAGACA
    CGGAGACACT CGTGGAGGCA GCAGATCTTC CTTCGGGTAG CCACCCCCCA GAAGGCCTGC
    GAGTCCCCCA AAAGATACGA AGATTACTCA GAGTTGGGAG AGCTTCCCCC ACGATCTCCA
    CTAGAACCAG TTTGTGAAGA TGGACCCTTT GGCCCGGTCC CAGAGGAAAA GAAAAGAACA
    TCTCATGAAC TTCGGGAGCT GTGGCAAAAG GCCATTCTAC AACAGATATT GCTGCTCAGG
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207896.1
    CDS732..4190
    Translation

    Target ORF information:

    RefSeq Version XM_005207839.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207839.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGGACTTTG AGTCCAAAGC CGACCACATC AGCGATGCCA GCAGGACCCC TGTGAAGACA
    CGGAGACACT CGTGGAGGCA GCAGATCTTC CTTCGGGTAG CCACCCCCCA GAAGGCCTGC
    GAGTCCCCCA AAAGATACGA AGATTACTCA GAGTTGGGAG AGCTTCCCCC ACGATCTCCA
    CTAGAACCAG TTTGTGAAGA TGGACCCTTT GGCCCGGTCC CAGAGGAAAA GAAAAGAACA
    TCTCATGAAC TTCGGGAGCT GTGGCAAAAG GCCATTCTAC AACAGATATT GCTGCTCAGG
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20338
    Clone ID Related Accession (Same CDS sequence) XM_005207839.4 , XM_005207839.3
    Accession Version XM_005207839.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3459bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207839.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGGACTTTG AGTCCAAAGC CGACCACATC AGCGATGCCA GCAGGACCCC TGTGAAGACA
    CGGAGACACT CGTGGAGGCA GCAGATCTTC CTTCGGGTAG CCACCCCCCA GAAGGCCTGC
    GAGTCCCCCA AAAGATACGA AGATTACTCA GAGTTGGGAG AGCTTCCCCC ACGATCTCCA
    CTAGAACCAG TTTGTGAAGA TGGACCCTTT GGCCCGGTCC CAGAGGAAAA GAAAAGAACA
    TCTCATGAAC TTCGGGAGCT GTGGCAAAAG GCCATTCTAC AACAGATATT GCTGCTCAGG
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207896.1
    CDS187..3645
    Translation

    Target ORF information:

    RefSeq Version XM_005207839.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207839.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGGACTTTG AGTCCAAAGC CGACCACATC AGCGATGCCA GCAGGACCCC TGTGAAGACA
    CGGAGACACT CGTGGAGGCA GCAGATCTTC CTTCGGGTAG CCACCCCCCA GAAGGCCTGC
    GAGTCCCCCA AAAGATACGA AGATTACTCA GAGTTGGGAG AGCTTCCCCC ACGATCTCCA
    CTAGAACCAG TTTGTGAAGA TGGACCCTTT GGCCCGGTCC CAGAGGAAAA GAAAAGAACA
    TCTCATGAAC TTCGGGAGCT GTGGCAAAAG GCCATTCTAC AACAGATATT GCTGCTCAGG
    ATGGAGAAAG AAAACCAAAA ACTGCAAGCC TCTGAGAATG ATTTGCTGAA CAAGCGCCTA
    AAGCTTGATT ATGAAGAAAT CACTCCCTGT CTTAAAGAAG TGACTACAGT GTGGGAAAAG
    ATACTTAGCA CTCCAGGAAG ATCAAAAATT AAGTTTGACA TGGAAAAAAT GCACTCGGCT
    GTTGGCCAAG GTGTGCCGCG ACACCACCGG GGTGAAATCT GGAAATTCTT GGCGGAGCAG
    TACCACCTGA AGCACCCGTT TCCCTGCAAA CAGCAGCCCA AGGACACGCC CTACAAAGAG
    CTCTTAAAGC AGCTCACCTC CCAGCAGCAC GCCATTCTCA TCGACCTTGG GCGAACCTTT
    CCAACACATC CGTACTACTC TGCTCAGCTA GGAGCAGGGC AGCTGTCACT TTACAACATC
    CTGAAGGCCT ACTCGCTTCT AGACCAAGAA GTGGGCTATT GCCAAGGTCT CAGCTTTGTA
    GCAGGCATCC TGCTGCTTCA TATGGGTGAA GAAGAGGCGT TTAACATGCT CAAGTTTCTG
    ATGTTTGACA TGGGACTGCG GAAACAATAT CGGCCGGACA TGATTATTTT ACAGATCCAG
    ATGTACCAGC TCTCGAGGCT GCTCCACGAC TACCACAGGG ACCTCTACAA CCACCTGGAG
    GAGCATGAGA TTGGCCCCAG CCTCTATGCT GCGCCCTGGT TCCTCACCGT GTTTGCCTCA
    CAGTTCCCCC TGGGCTTCGT CGCCAGAGTC TTTGATATGA TCTTTCTTCA GGGATCAGAG
    GTCATATTTA AAGTGGCTTT AAGTCTGCTG GGAAGCCATA AGCCCTTGAT TCTGCAGCAT
    GAAAACCTAG AAACCATAGT TGACTTTATA AAAAGCACAC TACCCAACCT GGGCTTGGTG
    CAGATGGAGA AGACTATCAG TCAGGTGTTT GAGACGGACA TCTCGAAACA GTTACAAGCT
    TATGAAGTTG AGTACCACGT GCTTCAGGAA GAGCTTATCG ATTCTTCTCC TCTCAGTGAC
    AACCAAAGAA TGGACAAACT AGAGAAGACC AACAGCAGCT TACGCAAACA GAACCTTGAC
    CTCCTTGAAC AGCTGCAGGT CGCCAATGGG AGGATCCAGA GCCTGGAAGC CACTGTTGAG
    AAGCTCCTGA CTAGCGAGAG CAAGCTGAAG CAGGCGACCC TGGCCTTGGA GCTGGAGAGG
    TCAGCCCTGC TGCAGACGGT GGAGCAGCTC CGGAGGCAGA CGGCAGAGCT GGGCAGCCAG
    GAGTCTGACC CCACACTGCC CAAGCCCTCA GGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20339
    Clone ID Related Accession (Same CDS sequence) XM_005207840.4 , XM_005207840.3
    Accession Version XM_005207840.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207840.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCACCAT TGTTCAGCTG GGTGGCCAAG GTGCCTGAGA TCATCAGTTC CATCCGGCAG 
    GCTGGGAAGA TCGCCCGCCA GGAGGAGCTC CGCTGCCCTT CGGAGTTCGA TGACACATTC
    GCCAAGAAGT TCGAGGTGCT GTTCTGCGGC CGCGTGGCCG TGGCGCACAG GAAGGCTCCA
    CCCGCCCTGA TCGATGAGTG CATCGAAGGC TTCAGTCACG TGAGCGGTGG CTTCTCCTCG
    GATCAGTCCC GGTCTGCACT GCAGCCACCG GGGGATGGGG AGCGGGGCCC GCGGCCGATG
    CGCAAGTCCT TCTCGCAGCC CGGGCTGCGC TCCTTGGCTT TCCGAAAGGA GTTTCAGGAT
    GCAGGCCTGC GGAGTAGTAG TTTCTTCAGC TCTTTTGAGG AGAGCGACAT CGAGAACCAC
    CTGATTAGTG GACACAATAT TGTGCAGCCG ACAGATATTG AGGAAAATCG AACTATGCTC
    TTCACGATTG GTCAATCTGA AGTTTACCTC ATCAGTCCTG ACACCAAAAA AATAGCGTTG
    CAGAAAAATT TTAAGGAGAT ATCCTTTTGC TCTCAGGGTA TTAGACATGT GGACCACTTT
    GGGTTCATCT GCCGTGAGTC TTCCGGAGGT GGAGGCTTTC ATTTCGTCTG TTACGTGTTT
    CAGTGCACAA ATGAAGCTCT GGTGGATGAG ATCATGATGA CCTTGAAGCA GGCCTTCACG
    GTGGCTGCTG TGCAGCAGAC AGCCAAGGCG CCAGCCCAGC TGTGTGAGGG CTGCCCCCTG
    CAGGGCCTGC ACAAGCTCTG CGAGCGGATC GAGGGAATGA ATTCTTCCAA AACCAAACTA
    GAACTCCAGA AGCACCTGAC AACATTAACT AATCAGGAGC AAGCAACTAT TTTTGAGGAG
    GTTCAGAAAT TGAGACCAAG AAATGAGCAA CGAGAGAATG AATTGATTAT TTCTTTCCTG
    AGATGTTTAT ATGAAGAGAA GCAAAAAGTT CACATCCATA TTGGGGAGAT AAAACAGACG
    TCACAGATTG CAGCAGAGAA TATTGGAAGC GAATTACCAT CAAGTGCCAC TCGATTTAGG
    CTAGATATGC TGAAAAACAA AGCGAAGAGG TCTTTAACAG AATCTTTAGA AAGTATTTTG
    TCTCGGGGTA ATAAAGCCAG AGGCCTCCAG GAACACTCAG CCAGTTTGGA TCTGGACAGC
    TCCGTGTCTA GCATGTTCAG TAACACCAGC AAAGAGCCGT CTGGCTATGA AAAGGAGGCC
    TTGCCCATCT CCGAGAGCTG CTTCAGGCTC CTCGGCTCCT CGGACGACCT GTCCAGTGAC
    TCAGAGAGCC AACTCACAGA AGAGCCAGCC CTGCTCTCGC CCAAGCAGGG CTTCCGAAGG
    CGCGCCAACA CCCTGAGTCA TGTTCCCGTG GAATGCCAGG AGCCTCCACA GCTGGTGCGA
    GGGTCTCCAG GGGTCTCGCA AAGGAAACTT GTGAGGTATC ACTCAGTGAG CACAGAGACG
    CCTCATGAAC GAAATGGGAA TTGTCCACCT GTTGGCGAGT CTCAAAGTCC CTCAGGTCAG
    TCTTCAGCTC CTGCTCCTCC ACCTCGTCTT AACCCCTCCG CCTCCTCGCC TAATTTTTTT
    AAGTACCTCA AACATAACCC GAGTGGAGAA CAGAGTGGGA ATGCCGTGCC AAAGAGCATC
    TCCTACCGTA ATGCCCTGCG GAAAAAACTT CATTCCTCTT CCTCTGTGCC AAATTTTCTA
    AAATTTCTGG CTCCTGTAGA TGAAAATAAC ACCTCTGACT TTATGAACAC TCAAAGGGAC
    TTTGAGTCCA AAGCCGACCA CATCAGCGAT GCCAGCAGGA CCCCTGTGAA GACACGGAGA
    CACTCGTGGA GGCAGCAGAT CTTCCTTCGG GTAGCCACCC CCCAGAAGGC CTGCGAGTCC
    CCCAAAAGAT ACGAAGATTA CTCAGAGTTG GGAGAGCTTC CCCCACGATC TCCACTAGAA
    CCAGTTTGTG AAGATGGACC CTTTGGCCCG GTCCCAGAGG AAAAGAAAAG AACATCTCAT
    GAACTTCGGG AGCTGTGGCA AAAGGCCATT CTACAACAGA TATTGCTGCT CAGGATGGAG
    AAAGAAAACC AAAAACTGCA AGCCTCTGAG AATGATTTGC TGAACAAGCG CCTAAAGCTT
    GATTATGAAG AAATCACTCC CTGTCTTAAA GAAGTGACTA CAGTGTGGGA AAAGATACTT
    AGCACTCCAG GAAGATCAAA AATTAAGTTT GACATGGAAA AAATGCACTC GGCTGTTGGC
    CAAGGTGTGC CGCGACACCA CCGGGGTGAA ATCTGGAAAT TCTTGGCGGA GCAGTACCAC
    CTGAAGCACC CGTTTCCCTG CAAACAGCAG CCCAAGGACA CGCCCTACAA AGAGCTCTTA
    AAGCAGCTCA CCTCCCAGCA GCACGCCATT CTCATCGACC TTGGGCGAAC CTTTCCAACA
    CATCCGTACT ACTCTGCTCA GCTAGGAGCA GGGCAGCTGT CACTTTACAA CATCCTGAAG
    GCCTACTCGC TTCTAGACCA AGAAGTGGGC TATTGCCAAG GTCTCAGCTT TGTAGCAGGC
    ATCCTGCTGC TTCATATGGG TGAAGAAGAG GCGTTTAACA TGCTCAAGTT TCTGATGTTT
    GACATGGGAC TGCGGAAACA ATATCGGCCG GACATGATTA TTTTACAGAT CCAGATGTAC
    CAGCTCTCGA GGCTGCTCCA CGACTACCAC AGGGACCTCT ACAACCACCT GGAGGAGCAT
    GAGATTGGCC CCAGCCTCTA TGCTGCGCCC TGGTTCCTCA CCGTGTTTGC CTCACAGTTC
    CCCCTGGGCT TCGTCGCCAG AGTCTTTGAT ATGATCTTTC TTCAGGGATC AGAGGTCATA
    TTTAAAGTGG CTTTAAGTCT GCTGGGAAGC CATAAGCCCT TGATTCTGCA GCATGAAAAC
    CTAGAAACCA TAGTTGACTT TATAAAAAGC ACACTACCCA ACCTGGGCTT GGTGCAGATG
    GAGAAGACTA TCAGTCAGGT GTTTGAGACG GACATCTCGA AACAGTTACA AGCTTATGAA
    GTTGAGTACC ACGTGCTTCA GGAAGAGCTT ATCGATTCTT CTCCTCTCAG TGACAACCAA
    AGAATGGACA AACTAGAGAA GACCAACAGC AGCTTACGCA AACAGAACCT TGACCTCCTT
    GAACAGCTGC AGGTCGCCAA TGGGAGGATC CAGAGCCTGG AAGCCACTGT TGAGAAGCTC
    CTGACTAGCG AGAGCAAGCT GAAGCAGGCG ACCCTGGCCT TGGAGCTGGA GAGGTCAGCC
    CTGCTGCAGA CGGTGGAGCA GCTCCGGAGG CAGACGGCAG AGCTGGGCAG CCAGGAGTCT
    GACCCCACAC TGCCCAAGCC CTCAGGTGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207897.1
    CDS164..3556
    Translation

    Target ORF information:

    RefSeq Version XM_005207840.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207840.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCACCAT TGTTCAGCTG GGTGGCCAAG GTGCCTGAGA TCATCAGTTC CATCCGGCAG 
    GCTGGGAAGA TCGCCCGCCA GGAGGAGCTC CGCTGCCCTT CGGAGTTCGA TGACACATTC
    GCCAAGAAGT TCGAGGTGCT GTTCTGCGGC CGCGTGGCCG TGGCGCACAG GAAGGCTCCA
    CCCGCCCTGA TCGATGAGTG CATCGAAGGC TTCAGTCACG TGAGCGGTGG CTTCTCCTCG
    GATCAGTCCC GGTCTGCACT GCAGCCACCG GGGGATGGGG AGCGGGGCCC GCGGCCGATG
    CGCAAGTCCT TCTCGCAGCC CGGGCTGCGC TCCTTGGCTT TCCGAAAGGA GTTTCAGGAT
    GCAGGCCTGC GGAGTAGTAG TTTCTTCAGC TCTTTTGAGG AGAGCGACAT CGAGAACCAC
    CTGATTAGTG GACACAATAT TGTGCAGCCG ACAGATATTG AGGAAAATCG AACTATGCTC
    TTCACGATTG GTCAATCTGA AGTTTACCTC ATCAGTCCTG ACACCAAAAA AATAGCGTTG
    CAGAAAAATT TTAAGGAGAT ATCCTTTTGC TCTCAGGGTA TTAGACATGT GGACCACTTT
    GGGTTCATCT GCCGTGAGTC TTCCGGAGGT GGAGGCTTTC ATTTCGTCTG TTACGTGTTT
    CAGTGCACAA ATGAAGCTCT GGTGGATGAG ATCATGATGA CCTTGAAGCA GGCCTTCACG
    GTGGCTGCTG TGCAGCAGAC AGCCAAGGCG CCAGCCCAGC TGTGTGAGGG CTGCCCCCTG
    CAGGGCCTGC ACAAGCTCTG CGAGCGGATC GAGGGAATGA ATTCTTCCAA AACCAAACTA
    GAACTCCAGA AGCACCTGAC AACATTAACT AATCAGGAGC AAGCAACTAT TTTTGAGGAG
    GTTCAGAAAT TGAGACCAAG AAATGAGCAA CGAGAGAATG AATTGATTAT TTCTTTCCTG
    AGATGTTTAT ATGAAGAGAA GCAAAAAGTT CACATCCATA TTGGGGAGAT AAAACAGACG
    TCACAGATTG CAGCAGAGAA TATTGGAAGC GAATTACCAT CAAGTGCCAC TCGATTTAGG
    CTAGATATGC TGAAAAACAA AGCGAAGAGG TCTTTAACAG AATCTTTAGA AAGTATTTTG
    TCTCGGGGTA ATAAAGCCAG AGGCCTCCAG GAACACTCAG CCAGTTTGGA TCTGGACAGC
    TCCGTGTCTA GCATGTTCAG TAACACCAGC AAAGAGCCGT CTGGCTATGA AAAGGAGGCC
    TTGCCCATCT CCGAGAGCTG CTTCAGGCTC CTCGGCTCCT CGGACGACCT GTCCAGTGAC
    TCAGAGAGCC AACTCACAGA AGAGCCAGCC CTGCTCTCGC CCAAGCAGGG CTTCCGAAGG
    CGCGCCAACA CCCTGAGTCA TGTTCCCGTG GAATGCCAGG AGCCTCCACA GCTGGTGCGA
    GGGTCTCCAG GGGTCTCGCA AAGGAAACTT GTGAGGTATC ACTCAGTGAG CACAGAGACG
    CCTCATGAAC GAAATGGGAA TTGTCCACCT GTTGGCGAGT CTCAAAGTCC CTCAGGTCAG
    TCTTCAGCTC CTGCTCCTCC ACCTCGTCTT AACCCCTCCG CCTCCTCGCC TAATTTTTTT
    AAGTACCTCA AACATAACCC GAGTGGAGAA CAGAGTGGGA ATGCCGTGCC AAAGAGCATC
    TCCTACCGTA ATGCCCTGCG GAAAAAACTT CATTCCTCTT CCTCTGTGCC AAATTTTCTA
    AAATTTCTGG CTCCTGTAGA TGAAAATAAC ACCTCTGACT TTATGAACAC TCAAAGGGAC
    TTTGAGTCCA AAGCCGACCA CATCAGCGAT GCCAGCAGGA CCCCTGTGAA GACACGGAGA
    CACTCGTGGA GGCAGCAGAT CTTCCTTCGG GTAGCCACCC CCCAGAAGGC CTGCGAGTCC
    CCCAAAAGAT ACGAAGATTA CTCAGAGTTG GGAGAGCTTC CCCCACGATC TCCACTAGAA
    CCAGTTTGTG AAGATGGACC CTTTGGCCCG GTCCCAGAGG AAAAGAAAAG AACATCTCAT
    GAACTTCGGG AGCTGTGGCA AAAGGCCATT CTACAACAGA TATTGCTGCT CAGGATGGAG
    AAAGAAAACC AAAAACTGCA AGCCTCTGAG AATGATTTGC TGAACAAGCG CCTAAAGCTT
    GATTATGAAG AAATCACTCC CTGTCTTAAA GAAGTGACTA CAGTGTGGGA AAAGATACTT
    AGCACTCCAG GAAGATCAAA AATTAAGTTT GACATGGAAA AAATGCACTC GGCTGTTGGC
    CAAGGTGTGC CGCGACACCA CCGGGGTGAA ATCTGGAAAT TCTTGGCGGA GCAGTACCAC
    CTGAAGCACC CGTTTCCCTG CAAACAGCAG CCCAAGGACA CGCCCTACAA AGAGCTCTTA
    AAGCAGCTCA CCTCCCAGCA GCACGCCATT CTCATCGACC TTGGGCGAAC CTTTCCAACA
    CATCCGTACT ACTCTGCTCA GCTAGGAGCA GGGCAGCTGT CACTTTACAA CATCCTGAAG
    GCCTACTCGC TTCTAGACCA AGAAGTGGGC TATTGCCAAG GTCTCAGCTT TGTAGCAGGC
    ATCCTGCTGC TTCATATGGG TGAAGAAGAG GCGTTTAACA TGCTCAAGTT TCTGATGTTT
    GACATGGGAC TGCGGAAACA ATATCGGCCG GACATGATTA TTTTACAGAT CCAGATGTAC
    CAGCTCTCGA GGCTGCTCCA CGACTACCAC AGGGACCTCT ACAACCACCT GGAGGAGCAT
    GAGATTGGCC CCAGCCTCTA TGCTGCGCCC TGGTTCCTCA CCGTGTTTGC CTCACAGTTC
    CCCCTGGGCT TCGTCGCCAG AGTCTTTGAT ATGATCTTTC TTCAGGGATC AGAGGTCATA
    TTTAAAGTGG CTTTAAGTCT GCTGGGAAGC CATAAGCCCT TGATTCTGCA GCATGAAAAC
    CTAGAAACCA TAGTTGACTT TATAAAAAGC ACACTACCCA ACCTGGGCTT GGTGCAGATG
    GAGAAGACTA TCAGTCAGGT GTTTGAGACG GACATCTCGA AACAGTTACA AGCTTATGAA
    GTTGAGTACC ACGTGCTTCA GGAAGAGCTT ATCGATTCTT CTCCTCTCAG TGACAACCAA
    AGAATGGACA AACTAGAGAA GACCAACAGC AGCTTACGCA AACAGAACCT TGACCTCCTT
    GAACAGCTGC AGGTCGCCAA TGGGAGGATC CAGAGCCTGG AAGCCACTGT TGAGAAGCTC
    CTGACTAGCG AGAGCAAGCT GAAGCAGGCG ACCCTGGCCT TGGAGCTGGA GAGGTCAGCC
    CTGCTGCAGA CGGTGGAGCA GCTCCGGAGG CAGACGGCAG AGCTGGGCAG CCAGGAGTCT
    GACCCCACAC TGCCCAAGCC CTCAGGTGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20339
    Clone ID Related Accession (Same CDS sequence) XM_005207840.4 , XM_005207840.3
    Accession Version XM_005207840.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207840.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCACCAT TGTTCAGCTG GGTGGCCAAG GTGCCTGAGA TCATCAGTTC CATCCGGCAG 
    GCTGGGAAGA TCGCCCGCCA GGAGGAGCTC CGCTGCCCTT CGGAGTTCGA TGACACATTC
    GCCAAGAAGT TCGAGGTGCT GTTCTGCGGC CGCGTGGCCG TGGCGCACAG GAAGGCTCCA
    CCCGCCCTGA TCGATGAGTG CATCGAAGGC TTCAGTCACG TGAGCGGTGG CTTCTCCTCG
    GATCAGTCCC GGTCTGCACT GCAGCCACCG GGGGATGGGG AGCGGGGCCC GCGGCCGATG
    CGCAAGTCCT TCTCGCAGCC CGGGCTGCGC TCCTTGGCTT TCCGAAAGGA GTTTCAGGAT
    GCAGGCCTGC GGAGTAGTAG TTTCTTCAGC TCTTTTGAGG AGAGCGACAT CGAGAACCAC
    CTGATTAGTG GACACAATAT TGTGCAGCCG ACAGATATTG AGGAAAATCG AACTATGCTC
    TTCACGATTG GTCAATCTGA AGTTTACCTC ATCAGTCCTG ACACCAAAAA AATAGCGTTG
    CAGAAAAATT TTAAGGAGAT ATCCTTTTGC TCTCAGGGTA TTAGACATGT GGACCACTTT
    GGGTTCATCT GCCGTGAGTC TTCCGGAGGT GGAGGCTTTC ATTTCGTCTG TTACGTGTTT
    CAGTGCACAA ATGAAGCTCT GGTGGATGAG ATCATGATGA CCTTGAAGCA GGCCTTCACG
    GTGGCTGCTG TGCAGCAGAC AGCCAAGGCG CCAGCCCAGC TGTGTGAGGG CTGCCCCCTG
    CAGGGCCTGC ACAAGCTCTG CGAGCGGATC GAGGGAATGA ATTCTTCCAA AACCAAACTA
    GAACTCCAGA AGCACCTGAC AACATTAACT AATCAGGAGC AAGCAACTAT TTTTGAGGAG
    GTTCAGAAAT TGAGACCAAG AAATGAGCAA CGAGAGAATG AATTGATTAT TTCTTTCCTG
    AGATGTTTAT ATGAAGAGAA GCAAAAAGTT CACATCCATA TTGGGGAGAT AAAACAGACG
    TCACAGATTG CAGCAGAGAA TATTGGAAGC GAATTACCAT CAAGTGCCAC TCGATTTAGG
    CTAGATATGC TGAAAAACAA AGCGAAGAGG TCTTTAACAG AATCTTTAGA AAGTATTTTG
    TCTCGGGGTA ATAAAGCCAG AGGCCTCCAG GAACACTCAG CCAGTTTGGA TCTGGACAGC
    TCCGTGTCTA GCATGTTCAG TAACACCAGC AAAGAGCCGT CTGGCTATGA AAAGGAGGCC
    TTGCCCATCT CCGAGAGCTG CTTCAGGCTC CTCGGCTCCT CGGACGACCT GTCCAGTGAC
    TCAGAGAGCC AACTCACAGA AGAGCCAGCC CTGCTCTCGC CCAAGCAGGG CTTCCGAAGG
    CGCGCCAACA CCCTGAGTCA TGTTCCCGTG GAATGCCAGG AGCCTCCACA GCTGGTGCGA
    GGGTCTCCAG GGGTCTCGCA AAGGAAACTT GTGAGGTATC ACTCAGTGAG CACAGAGACG
    CCTCATGAAC GAAATGGGAA TTGTCCACCT GTTGGCGAGT CTCAAAGTCC CTCAGGTCAG
    TCTTCAGCTC CTGCTCCTCC ACCTCGTCTT AACCCCTCCG CCTCCTCGCC TAATTTTTTT
    AAGTACCTCA AACATAACCC GAGTGGAGAA CAGAGTGGGA ATGCCGTGCC AAAGAGCATC
    TCCTACCGTA ATGCCCTGCG GAAAAAACTT CATTCCTCTT CCTCTGTGCC AAATTTTCTA
    AAATTTCTGG CTCCTGTAGA TGAAAATAAC ACCTCTGACT TTATGAACAC TCAAAGGGAC
    TTTGAGTCCA AAGCCGACCA CATCAGCGAT GCCAGCAGGA CCCCTGTGAA GACACGGAGA
    CACTCGTGGA GGCAGCAGAT CTTCCTTCGG GTAGCCACCC CCCAGAAGGC CTGCGAGTCC
    CCCAAAAGAT ACGAAGATTA CTCAGAGTTG GGAGAGCTTC CCCCACGATC TCCACTAGAA
    CCAGTTTGTG AAGATGGACC CTTTGGCCCG GTCCCAGAGG AAAAGAAAAG AACATCTCAT
    GAACTTCGGG AGCTGTGGCA AAAGGCCATT CTACAACAGA TATTGCTGCT CAGGATGGAG
    AAAGAAAACC AAAAACTGCA AGCCTCTGAG AATGATTTGC TGAACAAGCG CCTAAAGCTT
    GATTATGAAG AAATCACTCC CTGTCTTAAA GAAGTGACTA CAGTGTGGGA AAAGATACTT
    AGCACTCCAG GAAGATCAAA AATTAAGTTT GACATGGAAA AAATGCACTC GGCTGTTGGC
    CAAGGTGTGC CGCGACACCA CCGGGGTGAA ATCTGGAAAT TCTTGGCGGA GCAGTACCAC
    CTGAAGCACC CGTTTCCCTG CAAACAGCAG CCCAAGGACA CGCCCTACAA AGAGCTCTTA
    AAGCAGCTCA CCTCCCAGCA GCACGCCATT CTCATCGACC TTGGGCGAAC CTTTCCAACA
    CATCCGTACT ACTCTGCTCA GCTAGGAGCA GGGCAGCTGT CACTTTACAA CATCCTGAAG
    GCCTACTCGC TTCTAGACCA AGAAGTGGGC TATTGCCAAG GTCTCAGCTT TGTAGCAGGC
    ATCCTGCTGC TTCATATGGG TGAAGAAGAG GCGTTTAACA TGCTCAAGTT TCTGATGTTT
    GACATGGGAC TGCGGAAACA ATATCGGCCG GACATGATTA TTTTACAGAT CCAGATGTAC
    CAGCTCTCGA GGCTGCTCCA CGACTACCAC AGGGACCTCT ACAACCACCT GGAGGAGCAT
    GAGATTGGCC CCAGCCTCTA TGCTGCGCCC TGGTTCCTCA CCGTGTTTGC CTCACAGTTC
    CCCCTGGGCT TCGTCGCCAG AGTCTTTGAT ATGATCTTTC TTCAGGGATC AGAGGTCATA
    TTTAAAGTGG CTTTAAGTCT GCTGGGAAGC CATAAGCCCT TGATTCTGCA GCATGAAAAC
    CTAGAAACCA TAGTTGACTT TATAAAAAGC ACACTACCCA ACCTGGGCTT GGTGCAGATG
    GAGAAGACTA TCAGTCAGGT GTTTGAGACG GACATCTCGA AACAGTTACA AGCTTATGAA
    GTTGAGTACC ACGTGCTTCA GGAAGAGCTT ATCGATTCTT CTCCTCTCAG TGACAACCAA
    AGAATGGACA AACTAGAGAA GACCAACAGC AGCTTACGCA AACAGAACCT TGACCTCCTT
    GAACAGCTGC AGGTCGCCAA TGGGAGGATC CAGAGCCTGG AAGCCACTGT TGAGAAGCTC
    CTGACTAGCG AGAGCAAGCT GAAGCAGGCG ACCCTGGCCT TGGAGCTGGA GAGGTCAGCC
    CTGCTGCAGA CGGTGGAGCA GCTCCGGAGG CAGACGGCAG AGCTGGGCAG CCAGGAGTCT
    GACCCCACAC TGCCCAAGCC CTCAGGTGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207897.1
    CDS199..3591
    Translation

    Target ORF information:

    RefSeq Version XM_005207840.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207840.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGTCACCAT TGTTCAGCTG GGTGGCCAAG GTGCCTGAGA TCATCAGTTC CATCCGGCAG 
    GCTGGGAAGA TCGCCCGCCA GGAGGAGCTC CGCTGCCCTT CGGAGTTCGA TGACACATTC
    GCCAAGAAGT TCGAGGTGCT GTTCTGCGGC CGCGTGGCCG TGGCGCACAG GAAGGCTCCA
    CCCGCCCTGA TCGATGAGTG CATCGAAGGC TTCAGTCACG TGAGCGGTGG CTTCTCCTCG
    GATCAGTCCC GGTCTGCACT GCAGCCACCG GGGGATGGGG AGCGGGGCCC GCGGCCGATG
    CGCAAGTCCT TCTCGCAGCC CGGGCTGCGC TCCTTGGCTT TCCGAAAGGA GTTTCAGGAT
    GCAGGCCTGC GGAGTAGTAG TTTCTTCAGC TCTTTTGAGG AGAGCGACAT CGAGAACCAC
    CTGATTAGTG GACACAATAT TGTGCAGCCG ACAGATATTG AGGAAAATCG AACTATGCTC
    TTCACGATTG GTCAATCTGA AGTTTACCTC ATCAGTCCTG ACACCAAAAA AATAGCGTTG
    CAGAAAAATT TTAAGGAGAT ATCCTTTTGC TCTCAGGGTA TTAGACATGT GGACCACTTT
    GGGTTCATCT GCCGTGAGTC TTCCGGAGGT GGAGGCTTTC ATTTCGTCTG TTACGTGTTT
    CAGTGCACAA ATGAAGCTCT GGTGGATGAG ATCATGATGA CCTTGAAGCA GGCCTTCACG
    GTGGCTGCTG TGCAGCAGAC AGCCAAGGCG CCAGCCCAGC TGTGTGAGGG CTGCCCCCTG
    CAGGGCCTGC ACAAGCTCTG CGAGCGGATC GAGGGAATGA ATTCTTCCAA AACCAAACTA
    GAACTCCAGA AGCACCTGAC AACATTAACT AATCAGGAGC AAGCAACTAT TTTTGAGGAG
    GTTCAGAAAT TGAGACCAAG AAATGAGCAA CGAGAGAATG AATTGATTAT TTCTTTCCTG
    AGATGTTTAT ATGAAGAGAA GCAAAAAGTT CACATCCATA TTGGGGAGAT AAAACAGACG
    TCACAGATTG CAGCAGAGAA TATTGGAAGC GAATTACCAT CAAGTGCCAC TCGATTTAGG
    CTAGATATGC TGAAAAACAA AGCGAAGAGG TCTTTAACAG AATCTTTAGA AAGTATTTTG
    TCTCGGGGTA ATAAAGCCAG AGGCCTCCAG GAACACTCAG CCAGTTTGGA TCTGGACAGC
    TCCGTGTCTA GCATGTTCAG TAACACCAGC AAAGAGCCGT CTGGCTATGA AAAGGAGGCC
    TTGCCCATCT CCGAGAGCTG CTTCAGGCTC CTCGGCTCCT CGGACGACCT GTCCAGTGAC
    TCAGAGAGCC AACTCACAGA AGAGCCAGCC CTGCTCTCGC CCAAGCAGGG CTTCCGAAGG
    CGCGCCAACA CCCTGAGTCA TGTTCCCGTG GAATGCCAGG AGCCTCCACA GCTGGTGCGA
    GGGTCTCCAG GGGTCTCGCA AAGGAAACTT GTGAGGTATC ACTCAGTGAG CACAGAGACG
    CCTCATGAAC GAAATGGGAA TTGTCCACCT GTTGGCGAGT CTCAAAGTCC CTCAGGTCAG
    TCTTCAGCTC CTGCTCCTCC ACCTCGTCTT AACCCCTCCG CCTCCTCGCC TAATTTTTTT
    AAGTACCTCA AACATAACCC GAGTGGAGAA CAGAGTGGGA ATGCCGTGCC AAAGAGCATC
    TCCTACCGTA ATGCCCTGCG GAAAAAACTT CATTCCTCTT CCTCTGTGCC AAATTTTCTA
    AAATTTCTGG CTCCTGTAGA TGAAAATAAC ACCTCTGACT TTATGAACAC TCAAAGGGAC
    TTTGAGTCCA AAGCCGACCA CATCAGCGAT GCCAGCAGGA CCCCTGTGAA GACACGGAGA
    CACTCGTGGA GGCAGCAGAT CTTCCTTCGG GTAGCCACCC CCCAGAAGGC CTGCGAGTCC
    CCCAAAAGAT ACGAAGATTA CTCAGAGTTG GGAGAGCTTC CCCCACGATC TCCACTAGAA
    CCAGTTTGTG AAGATGGACC CTTTGGCCCG GTCCCAGAGG AAAAGAAAAG AACATCTCAT
    GAACTTCGGG AGCTGTGGCA AAAGGCCATT CTACAACAGA TATTGCTGCT CAGGATGGAG
    AAAGAAAACC AAAAACTGCA AGCCTCTGAG AATGATTTGC TGAACAAGCG CCTAAAGCTT
    GATTATGAAG AAATCACTCC CTGTCTTAAA GAAGTGACTA CAGTGTGGGA AAAGATACTT
    AGCACTCCAG GAAGATCAAA AATTAAGTTT GACATGGAAA AAATGCACTC GGCTGTTGGC
    CAAGGTGTGC CGCGACACCA CCGGGGTGAA ATCTGGAAAT TCTTGGCGGA GCAGTACCAC
    CTGAAGCACC CGTTTCCCTG CAAACAGCAG CCCAAGGACA CGCCCTACAA AGAGCTCTTA
    AAGCAGCTCA CCTCCCAGCA GCACGCCATT CTCATCGACC TTGGGCGAAC CTTTCCAACA
    CATCCGTACT ACTCTGCTCA GCTAGGAGCA GGGCAGCTGT CACTTTACAA CATCCTGAAG
    GCCTACTCGC TTCTAGACCA AGAAGTGGGC TATTGCCAAG GTCTCAGCTT TGTAGCAGGC
    ATCCTGCTGC TTCATATGGG TGAAGAAGAG GCGTTTAACA TGCTCAAGTT TCTGATGTTT
    GACATGGGAC TGCGGAAACA ATATCGGCCG GACATGATTA TTTTACAGAT CCAGATGTAC
    CAGCTCTCGA GGCTGCTCCA CGACTACCAC AGGGACCTCT ACAACCACCT GGAGGAGCAT
    GAGATTGGCC CCAGCCTCTA TGCTGCGCCC TGGTTCCTCA CCGTGTTTGC CTCACAGTTC
    CCCCTGGGCT TCGTCGCCAG AGTCTTTGAT ATGATCTTTC TTCAGGGATC AGAGGTCATA
    TTTAAAGTGG CTTTAAGTCT GCTGGGAAGC CATAAGCCCT TGATTCTGCA GCATGAAAAC
    CTAGAAACCA TAGTTGACTT TATAAAAAGC ACACTACCCA ACCTGGGCTT GGTGCAGATG
    GAGAAGACTA TCAGTCAGGT GTTTGAGACG GACATCTCGA AACAGTTACA AGCTTATGAA
    GTTGAGTACC ACGTGCTTCA GGAAGAGCTT ATCGATTCTT CTCCTCTCAG TGACAACCAA
    AGAATGGACA AACTAGAGAA GACCAACAGC AGCTTACGCA AACAGAACCT TGACCTCCTT
    GAACAGCTGC AGGTCGCCAA TGGGAGGATC CAGAGCCTGG AAGCCACTGT TGAGAAGCTC
    CTGACTAGCG AGAGCAAGCT GAAGCAGGCG ACCCTGGCCT TGGAGCTGGA GAGGTCAGCC
    CTGCTGCAGA CGGTGGAGCA GCTCCGGAGG CAGACGGCAG AGCTGGGCAG CCAGGAGTCT
    GACCCCACAC TGCCCAAGCC CTCAGGTGAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20340
    Clone ID Related Accession (Same CDS sequence) XM_005207841.3 , XM_005207841.4
    Accession Version XM_005207841.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005207841.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207898.1
    CDS191..3433
    Translation

    Target ORF information:

    RefSeq Version XM_005207841.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207841.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa20340
    Clone ID Related Accession (Same CDS sequence) XM_005207841.3 , XM_005207841.4
    Accession Version XM_005207841.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3243bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005207841.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005207898.1
    CDS323..3565
    Translation

    Target ORF information:

    RefSeq Version XM_005207841.4
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005207841.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGTGTT
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45492
    Clone ID Related Accession (Same CDS sequence) XM_010806055.2
    Accession Version XM_010806055.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3660bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806055.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGATATG
    ATCTTTCTTC AGGGATCAGA GGTCATATTT AAAGTGGCTT TAAGTCTGCT GGGAAGCCAT
    AAGCCCTTGA TTCTGCAGCA TGAAAACCTA GAAACCATAG TTGACTTTAT AAAAAGCACA
    CTACCCAACC TGGGCTTGGT GCAGATGGAG AAGACTATCA GTCAGGTGTT TGAGACGGAC
    ATCTCGAAAC AGTTACAAGC TTATGAAGTT GAGTACCACG TGCTTCAGGA AGAGCTTATC
    GATTCTTCTC CTCTCAGTGA CAACCAAAGA ATGGACAAAC TAGAGAAGAC CAACAGCAGC
    TTACGCAAAC AGAACCTTGA CCTCCTTGAA CAGCTGCAGG TCGCCAATGG GAGGATCCAG
    AGCCTGGAAG CCACTGTTGA GAAGCTCCTG ACTAGCGAGA GCAAGCTGAA GCAGGCGACC
    CTGGCCTTGG AGCTGGAGAG GTCAGCCCTG CTGCAGACGG TGGAGCAGCT CCGGAGGCAG
    ACGGCAGAGC TGGGCAGCCA GGAGTCTGAC CCCACACTGC CCAAGCCCTC AGGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804357.1
    CDS187..3846
    Translation

    Target ORF information:

    RefSeq Version XM_010806055.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806055.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGATATG
    ATCTTTCTTC AGGGATCAGA GGTCATATTT AAAGTGGCTT TAAGTCTGCT GGGAAGCCAT
    AAGCCCTTGA TTCTGCAGCA TGAAAACCTA GAAACCATAG TTGACTTTAT AAAAAGCACA
    CTACCCAACC TGGGCTTGGT GCAGATGGAG AAGACTATCA GTCAGGTGTT TGAGACGGAC
    ATCTCGAAAC AGTTACAAGC TTATGAAGTT GAGTACCACG TGCTTCAGGA AGAGCTTATC
    GATTCTTCTC CTCTCAGTGA CAACCAAAGA ATGGACAAAC TAGAGAAGAC CAACAGCAGC
    TTACGCAAAC AGAACCTTGA CCTCCTTGAA CAGCTGCAGG TCGCCAATGG GAGGATCCAG
    AGCCTGGAAG CCACTGTTGA GAAGCTCCTG ACTAGCGAGA GCAAGCTGAA GCAGGCGACC
    CTGGCCTTGG AGCTGGAGAG GTCAGCCCTG CTGCAGACGG TGGAGCAGCT CCGGAGGCAG
    ACGGCAGAGC TGGGCAGCCA GGAGTCTGAC CCCACACTGC CCAAGCCCTC AGGTGACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45493
    Clone ID Related Accession (Same CDS sequence) XM_010806056.2 , XM_010806056.3
    Accession Version XM_010806056.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-01-25
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103973.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010806056.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGCTCT
    CACTGGAGCA GATGTCCTGA AGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804358.1
    CDS191..3457
    Translation

    Target ORF information:

    RefSeq Version XM_010806056.2
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806056.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGCTCT
    CACTGGAGCA GATGTCCTGA AGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa45493
    Clone ID Related Accession (Same CDS sequence) XM_010806056.2 , XM_010806056.3
    Accession Version XM_010806056.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3267bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product TBC1 domain family member 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037333.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_010806056.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGCTCT
    CACTGGAGCA GATGTCCTGA AGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_010804358.1
    CDS323..3589
    Translation

    Target ORF information:

    RefSeq Version XM_010806056.3
    Organism Bos taurus(cattle)
    Definition Bos taurus TBC1 domain family member 1 (TBC1D1), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_010806056.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGGAACCAA TAACCTTCAC AGCAAGGAAA CACCCATTCC CTAACGAGGT CTCCGTGGAC 
    TTCGGCCTCC AGCTGGTGGG CTCCTTGCCT GTGCATTCTC TGACTACCAT GCCCATGCTG
    CCGTGGGTGG TGGCAGAGGT GCGAAGGCTC AGCGGGCAGT CTTCCAAAAA GGAGCCTGGC
    ACCAAGCCAG TCCGCCTTTG TGTTTCGCCC TCTGGATTGA GATGTGAGCC TGAGCCAGGA
    AAAAGTCAAC AATGGGATCC ATTGATCTGT TCCAGTATCT TCGAGTGTAA GCCTCAACGA
    GTTCACAAAC TGATTCACAA TAGTCACGAC CCAAGTTATT TTGCTTGTCT GATTAAAAAT
    GATGCAGCCA ATCAGCAGAG TATCTGCTAT GTGTTCAAAG CCGATGATCA AACAAAAGTG
    CCTGAGATCA TCAGTTCCAT CCGGCAGGCT GGGAAGATCG CCCGCCAGGA GGAGCTCCGC
    TGCCCTTCGG AGTTCGATGA CACATTCGCC AAGAAGTTCG AGGTGCTGTT CTGCGGCCGC
    GTGGCCGTGG CGCACAGGAA GGCTCCACCC GCCCTGATCG ATGAGTGCAT CGAAGGCTTC
    AGTCACGTGA GCGGTGGCTT CTCCTCGGAT CAGTCCCGGT CTGCACTGCA GCCACCGGGG
    GATGGGGAGC GGGGCCCGCG GCCGATGCGC AAGTCCTTCT CGCAGCCCGG GCTGCGCTCC
    TTGGCTTTCC GAAAGGAGTT TCAGGATGCA GGCCTGCGGA GTAGTAGTTT CTTCAGCTCT
    TTTGAGGAGA GCGACATCGA GAACCACCTG ATTAGTGGAC ACAATATTGT GCAGCCGACA
    GATATTGAGG AAAATCGAAC TATGCTCTTC ACGATTGGTC AATCTGAAGT TTACCTCATC
    AGTCCTGACA CCAAAAAAAT AGCGTTGCAG AAAAATTTTA AGGAGATATC CTTTTGCTCT
    CAGGGTATTA GACATGTGGA CCACTTTGGG TTCATCTGCC GTGAGTCTTC CGGAGGTGGA
    GGCTTTCATT TCGTCTGTTA CGTGTTTCAG TGCACAAATG AAGCTCTGGT GGATGAGATC
    ATGATGACCT TGAAGCAGGC CTTCACGGTG GCTGCTGTGC AGCAGACAGC CAAGGCGCCA
    GCCCAGCTGT GTGAGGGCTG CCCCCTGCAG GGCCTGCACA AGCTCTGCGA GCGGATCGAG
    GGAATGAATT CTTCCAAAAC CAAACTAGAA CTCCAGAAGC ACCTGACAAC ATTAACTAAT
    CAGGAGCAAG CAACTATTTT TGAGGAGGTT CAGAAATTGA GACCAAGAAA TGAGCAACGA
    GAGAATGAAT TGATTATTTC TTTCCTGAGA TGTTTATATG AAGAGAAGCA AAAAGTTCAC
    ATCCATATTG GGGAGATAAA ACAGACGTCA CAGATTGCAG CAGAGAATAT TGGAAGCGAA
    TTACCATCAA GTGCCACTCG ATTTAGGCTA GATATGCTGA AAAACAAAGC GAAGAGGTCT
    TTAACAGAAT CTTTAGAAAG TATTTTGTCT CGGGGTAATA AAGCCAGAGG CCTCCAGGAA
    CACTCAGCCA GTTTGGATCT GGACAGCTCC GTGTCTAGCA TGTTCAGTAA CACCAGCAAA
    GAGCCGTCTG GCTATGAAAA GGAGGCCTTG CCCATCTCCG AGAGCTGCTT CAGGCTCCTC
    GGCTCCTCGG ACGACCTGTC CAGTGACTCA GAGAGCCAAC TCACAGAAGA GCCAGCCCTG
    CTCTCGCCCA AGCAGGGCTT CCGAAGGCGC GCCAACACCC TGAGTCATGT TCCCGTGGAA
    TGCCAGGAGC CTCCACAGCT GGTGCGAGGG TCTCCAGGGG TCTCGCAAAG GAAACTTGTG
    AGGTATCACT CAGTGAGCAC AGAGACGCCT CATGAACGAA ATGGGAATTG TCCACCTGTT
    GGCGAGTCTC AAAGTCCCTC AGGTCAGTCT TCAGCTCCTG CTCCTCCACC TCGTCTTAAC
    CCCTCCGCCT CCTCGCCTAA TTTTTTTAAG TACCTCAAAC ATAACCCGAG TGGAGAACAG
    AGTGGGAATG CCGTGCCAAA GAGCATCTCC TACCGTAATG CCCTGCGGAA AAAACTTCAT
    TCCTCTTCCT CTGTGCCAAA TTTTCTAAAA TTTCTGGCTC CTGTAGATGA AAATAACACC
    TCTGACTTTA TGAACACTCA AAGGGACTTT GAGTCCAAAG CCGACCACAT CAGCGATGCC
    AGCAGGACCC CTGTGAAGAC ACGGAGACAC TCGTGGAGGC AGCAGATCTT CCTTCGGGTA
    GCCACCCCCC AGAAGGCCTG CGAGTCCCCC AAAAGATACG AAGATTACTC AGAGTTGGGA
    GAGCTTCCCC CACGATCTCC ACTAGAACCA GTTTGTGAAG ATGGACCCTT TGGCCCGGTC
    CCAGAGGAAA AGAAAAGAAC ATCTCATGAA CTTCGGGAGC TGTGGCAAAA GGCCATTCTA
    CAACAGATAT TGCTGCTCAG GATGGAGAAA GAAAACCAAA AACTGCAAGC CTCTGAGAAT
    GATTTGCTGA ACAAGCGCCT AAAGCTTGAT TATGAAGAAA TCACTCCCTG TCTTAAAGAA
    GTGACTACAG TGTGGGAAAA GATACTTAGC ACTCCAGGAA GATCAAAAAT TAAGTTTGAC
    ATGGAAAAAA TGCACTCGGC TGTTGGCCAA GGTGTGCCGC GACACCACCG GGGTGAAATC
    TGGAAATTCT TGGCGGAGCA GTACCACCTG AAGCACCCGT TTCCCTGCAA ACAGCAGCCC
    AAGGACACGC CCTACAAAGA GCTCTTAAAG CAGCTCACCT CCCAGCAGCA CGCCATTCTC
    ATCGACCTTG GGCGAACCTT TCCAACACAT CCGTACTACT CTGCTCAGCT AGGAGCAGGG
    CAGCTGTCAC TTTACAACAT CCTGAAGGCC TACTCGCTTC TAGACCAAGA AGTGGGCTAT
    TGCCAAGGTC TCAGCTTTGT AGCAGGCATC CTGCTGCTTC ATATGGGTGA AGAAGAGGCG
    TTTAACATGC TCAAGTTTCT GATGTTTGAC ATGGGACTGC GGAAACAATA TCGGCCGGAC
    ATGATTATTT TACAGATCCA GATGTACCAG CTCTCGAGGC TGCTCCACGA CTACCACAGG
    GACCTCTACA ACCACCTGGA GGAGCATGAG ATTGGCCCCA GCCTCTATGC TGCGCCCTGG
    TTCCTCACCG TGTTTGCCTC ACAGTTCCCC CTGGGCTTCG TCGCCAGAGT CTTTGGCTCT
    CACTGGAGCA GATGTCCTGA AGGCTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.