tbc1d1 cDNA ORF clone, Danio rerio(zebrafish)

The following tbc1d1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tbc1d1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa53873 XM_017356001.2
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa53873 XM_021476250.1
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa05629 XM_005168369.4
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa05629 XM_021476243.1
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa05631 XM_021476251.1
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa05631 XM_684525.9
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa05632 XM_005168371.4
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa05632 XM_021476253.1
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43457 XM_021476256.1
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43457 XM_009307533.3
Latest version!
Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa05631 XM_684525.8 Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa05632 XM_005168371.3 Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa43457 XM_009307533.2 Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa05629 XM_005168369.3 Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa53873 XM_017356001.1 Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa53873
    Clone ID Related Accession (Same CDS sequence) XM_017356001.1 , XM_017356001.2 , XM_021476250.1
    Accession Version XM_017356001.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product TBC1 domain family member 1 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211490.1
    CDS235..3945
    Translation

    Target ORF information:

    RefSeq Version XM_017356001.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53873
    Clone ID Related Accession (Same CDS sequence) XM_017356001.1 , XM_017356001.2 , XM_021476250.1
    Accession Version XM_017356001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394473.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017211490.1
    CDS254..3964
    Translation

    Target ORF information:

    RefSeq Version XM_017356001.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017356001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa53873
    Clone ID Related Accession (Same CDS sequence) XM_017356001.1 , XM_017356001.2 , XM_021476250.1
    Accession Version XM_021476250.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331925.1
    CDS253..3963
    Translation

    Target ORF information:

    RefSeq Version XM_021476250.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476250.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05629
    Clone ID Related Accession (Same CDS sequence) XM_021476243.1 , XM_005168369.3 , XM_005168369.4
    Accession Version XM_021476243.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTC CATCAGGGCT GAGTGGCTTT AGAGCGAGAT TACACTCGTC CTCCTCTGTT
    CCAAATTTCC TCAAATTTCT GGCCCCTGTA GTGGAAAGTG ATGATACCTG TGACTCTCAG
    AGGAAGAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331918.1
    CDS253..4083
    Translation

    Target ORF information:

    RefSeq Version XM_021476243.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476243.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTC CATCAGGGCT GAGTGGCTTT AGAGCGAGAT TACACTCGTC CTCCTCTGTT
    CCAAATTTCC TCAAATTTCT GGCCCCTGTA GTGGAAAGTG ATGATACCTG TGACTCTCAG
    AGGAAGAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05629
    Clone ID Related Accession (Same CDS sequence) XM_021476243.1 , XM_005168369.3 , XM_005168369.4
    Accession Version XM_005168369.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product TBC1 domain family member 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168369.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTC CATCAGGGCT GAGTGGCTTT AGAGCGAGAT TACACTCGTC CTCCTCTGTT
    CCAAATTTCC TCAAATTTCT GGCCCCTGTA GTGGAAAGTG ATGATACCTG TGACTCTCAG
    AGGAAGAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168426.1
    CDS235..4065
    Translation

    Target ORF information:

    RefSeq Version XM_005168369.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168369.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTC CATCAGGGCT GAGTGGCTTT AGAGCGAGAT TACACTCGTC CTCCTCTGTT
    CCAAATTTCC TCAAATTTCT GGCCCCTGTA GTGGAAAGTG ATGATACCTG TGACTCTCAG
    AGGAAGAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05629
    Clone ID Related Accession (Same CDS sequence) XM_021476243.1 , XM_005168369.3 , XM_005168369.4
    Accession Version XM_005168369.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3831bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394473.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168369.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTC CATCAGGGCT GAGTGGCTTT AGAGCGAGAT TACACTCGTC CTCCTCTGTT
    CCAAATTTCC TCAAATTTCT GGCCCCTGTA GTGGAAAGTG ATGATACCTG TGACTCTCAG
    AGGAAGAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168426.1
    CDS254..4084
    Translation

    Target ORF information:

    RefSeq Version XM_005168369.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168369.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT CCTTCCTCCT CTCCGGCTTC TTCCTCTCCT
    GTGTGTCAGC GGCCGCTGAG GCCTCGTTCT TTTGCTCTTG ATCATTCCAC ACGGCCTCTC
    AAGCGCAGTC CATCAGGGCT GAGTGGCTTT AGAGCGAGAT TACACTCGTC CTCCTCTGTT
    CCAAATTTCC TCAAATTTCT GGCCCCTGTA GTGGAAAGTG ATGATACCTG TGACTCTCAG
    AGGAAGAGTG ATGTTGCGGC CCTTTCCAGT CCTGGATGTG TTATTGGTGA TGACAGTCCA
    TTGCGCAACC GGAGACACTC ATGGAGACAA CAGATCTTCT TAAGAGTCGC TACACCACAG
    AAGGGCTCGG ACGCCAAAGA ATCCACGTTA GAGGTTGGCA GCAGAATGGC ACTGGGAGGT
    GGAGTTGGTG GCGGAGACAA AACACTTGGG GCGGTGCCAG AGGAGAGGAG AAAGAGGAGC
    GGGGCGGAGC TTAGGAAGTT ATGGAGGAAA GCCATTCTTC AACAGATACT CCTACTGCGG
    ATGGAGAAAG AGAATCAGAA ACTACAAGCG TCAGAGAGTG ATCTGCAGTG CAAGCGACAG
    AAGCTGGATT ATGAAGAGAT CACTCCATGC CTGAAAGATG TCACTCTGGT CTGGGAGAGG
    ATGCTCGGCA CGGCTGGAAG AGCAAAGGTC AGATTTGACA TGGATGAGAT CCACGGTGCT
    GTGGGACAAG GCGTTCCTCG TCAGCATCGA GGAGAGATCT GGAAGTTTCT CTCTGAGCAG
    TATTTGCTGA GGCAGGAGGT GCCCAGTGCC AAACCGCCAA ACAATGACAC TCCTTACAAA
    GAGCTACTGA AGCAGCTCAC ATCCCAGCAG CATGCCATCC TCATTGACTT GGGTCGTACA
    TTTCCCACTC ACCCGTATTT CAGCGCTCAG CTTGGTGCAG GCCAGCTGTC CCTCTATAAC
    CTACTGAAGG CATATTCTCT GCTGGACCCG GAGGTCGGTT ACTGCCAGGG CCTGAGCTTT
    GTGGCTGGTG TTTTGCTGCT CCACATGAGC GAGGAAGACG CGTTTCACAT GCTCAAATTC
    CTCATGTACG ACATGGGGCT CAGGAAACAG TACAGGCCTG ATATGATCAT ACTGCAGATT
    CAGATGTATC AGCTGTCTCG TCTGTTGCAT GACTACCACA GGGAGCTCTA CTCTCATCTG
    GAGCTTTATG AGATCGGCCC CAGTCTCTAC GCCGCTCCAT GGTTCCTCAC CGCCTTTGCC
    TCACACTTCC CGCTCGGATT TGTGGCAAGA GTATTCGACA TGCTGTTTCT TCAGGGGTCA
    GAGGTCATCT TCAAAGTGGC CTTGAGTCTT CTCGGGAGCC ACAAACCTCT GATTCTCCAA
    CACGACAATC TTGAGGCAAT CGTGGAGTTC ATCAAAACAA CCTTACCCAA CCTCGGCCTC
    GTGCAAATGG AGAAAACTAT CAATCAGGTG TCAGAGATGG ATATCAGTAA GCAGCTGCAG
    GCCTATGAAG TGGAATATCA TGTTCTTCAG GATGAGCTTT TGGATGGTCC CTCCACACTT
    AGCCATTCCC AGCGAGCGGC CCAGTTGGAG AAGACCAATG GAAGTCTCCG ACAACAAAAC
    TTAGACCTCC TAGAGGAGCT ACAGGTTGCC CACGCTAAAA TCAGATGTTT AGAGACTCGT
    GTGGACGGCC TTGTGAACCG AGAGGCTGAG CTGCAATCAC AAGTAAAGAC TCTCCAGCAG
    GAACAAACAG ATCTCCAGCA GACCGTATGC AAACTCAGAG ACCTTCTCAG CAGTCTGGGC
    ATAAGCAACC CCTGCCCAGA CCTCCAAATC CTCACAAACA ACAACCAATA A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05631
    Clone ID Related Accession (Same CDS sequence) XM_684525.9 , XM_684525.8 , XM_021476251.1
    Accession Version XM_684525.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394473.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_684525.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT GATGTTGCGG CCCTTTCCAG TCCTGGATGT
    GTTATTGGTG ATGACAGTCC ATTGCGCAAC CGGAGACACT CATGGAGACA ACAGATCTTC
    TTAAGAGTCG CTACACCACA GAAGGGCTCG GACGCCAAAG AATCCACGTT AGAGGTTGGC
    AGCAGAATGG CACTGGGAGG TGGAGTTGGT GGCGGAGACA AAACACTTGG GGCGGTGCCA
    GAGGAGAGGA GAAAGAGGAG CGGGGCGGAG CTTAGGAAGT TATGGAGGAA AGCCATTCTT
    CAACAGATAC TCCTACTGCG GATGGAGAAA GAGAATCAGA AACTACAAGC GTCAGAGAGT
    GATCTGCAGT GCAAGCGACA GAAGCTGGAT TATGAAGAGA TCACTCCATG CCTGAAAGAT
    GTCACTCTGG TCTGGGAGAG GATGCTCGGC ACGGCTGGAA GAGCAAAGGT CAGATTTGAC
    ATGGATGAGA TCCACGGTGC TGTGGGACAA GGCGTTCCTC GTCAGCATCG AGGAGAGATC
    TGGAAGTTTC TCTCTGAGCA GTATTTGCTG AGGCAGGAGG TGCCCAGTGC CAAACCGCCA
    AACAATGACA CTCCTTACAA AGAGCTACTG AAGCAGCTCA CATCCCAGCA GCATGCCATC
    CTCATTGACT TGGGTCGTAC ATTTCCCACT CACCCGTATT TCAGCGCTCA GCTTGGTGCA
    GGCCAGCTGT CCCTCTATAA CCTACTGAAG GCATATTCTC TGCTGGACCC GGAGGTCGGT
    TACTGCCAGG GCCTGAGCTT TGTGGCTGGT GTTTTGCTGC TCCACATGAG CGAGGAAGAC
    GCGTTTCACA TGCTCAAATT CCTCATGTAC GACATGGGGC TCAGGAAACA GTACAGGCCT
    GATATGATCA TACTGCAGAT TCAGATGTAT CAGCTGTCTC GTCTGTTGCA TGACTACCAC
    AGGGAGCTCT ACTCTCATCT GGAGCTTTAT GAGATCGGCC CCAGTCTCTA CGCCGCTCCA
    TGGTTCCTCA CCGCCTTTGC CTCACACTTC CCGCTCGGAT TTGTGGCAAG AGTATTCGAC
    ATGCTGTTTC TTCAGGGGTC AGAGGTCATC TTCAAAGTGG CCTTGAGTCT TCTCGGGAGC
    CACAAACCTC TGATTCTCCA ACACGACAAT CTTGAGGCAA TCGTGGAGTT CATCAAAACA
    ACCTTACCCA ACCTCGGCCT CGTGCAAATG GAGAAAACTA TCAATCAGGT GTCAGAGATG
    GATATCAGTA AGCAGCTGCA GGCCTATGAA GTGGAATATC ATGTTCTTCA GGATGAGCTT
    TTGGATGGTC CCTCCACACT TAGCCATTCC CAGCGAGCGG CCCAGTTGGA GAAGACCAAT
    GGAAGTCTCC GACAACAAAA CTTAGACCTC CTAGAGGAGC TACAGGTTGC CCACGCTAAA
    ATCAGATGTT TAGAGACTCG TGTGGACGGC CTTGTGAACC GAGAGGCTGA GCTGCAATCA
    CAAGTAAAGA CTCTCCAGCA GGAACAAACA GATCTCCAGC AGACCGTATG CAAACTCAGA
    GACCTTCTCA GCAGTCTGGG CATAAGCAAC CCCTGCCCAG ACCTCCAAAT CCTCACAAAC
    AACAACCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_689617.6
    CDS254..3865
    Translation

    Target ORF information:

    RefSeq Version XM_684525.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_684525.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT GATGTTGCGG CCCTTTCCAG TCCTGGATGT
    GTTATTGGTG ATGACAGTCC ATTGCGCAAC CGGAGACACT CATGGAGACA ACAGATCTTC
    TTAAGAGTCG CTACACCACA GAAGGGCTCG GACGCCAAAG AATCCACGTT AGAGGTTGGC
    AGCAGAATGG CACTGGGAGG TGGAGTTGGT GGCGGAGACA AAACACTTGG GGCGGTGCCA
    GAGGAGAGGA GAAAGAGGAG CGGGGCGGAG CTTAGGAAGT TATGGAGGAA AGCCATTCTT
    CAACAGATAC TCCTACTGCG GATGGAGAAA GAGAATCAGA AACTACAAGC GTCAGAGAGT
    GATCTGCAGT GCAAGCGACA GAAGCTGGAT TATGAAGAGA TCACTCCATG CCTGAAAGAT
    GTCACTCTGG TCTGGGAGAG GATGCTCGGC ACGGCTGGAA GAGCAAAGGT CAGATTTGAC
    ATGGATGAGA TCCACGGTGC TGTGGGACAA GGCGTTCCTC GTCAGCATCG AGGAGAGATC
    TGGAAGTTTC TCTCTGAGCA GTATTTGCTG AGGCAGGAGG TGCCCAGTGC CAAACCGCCA
    AACAATGACA CTCCTTACAA AGAGCTACTG AAGCAGCTCA CATCCCAGCA GCATGCCATC
    CTCATTGACT TGGGTCGTAC ATTTCCCACT CACCCGTATT TCAGCGCTCA GCTTGGTGCA
    GGCCAGCTGT CCCTCTATAA CCTACTGAAG GCATATTCTC TGCTGGACCC GGAGGTCGGT
    TACTGCCAGG GCCTGAGCTT TGTGGCTGGT GTTTTGCTGC TCCACATGAG CGAGGAAGAC
    GCGTTTCACA TGCTCAAATT CCTCATGTAC GACATGGGGC TCAGGAAACA GTACAGGCCT
    GATATGATCA TACTGCAGAT TCAGATGTAT CAGCTGTCTC GTCTGTTGCA TGACTACCAC
    AGGGAGCTCT ACTCTCATCT GGAGCTTTAT GAGATCGGCC CCAGTCTCTA CGCCGCTCCA
    TGGTTCCTCA CCGCCTTTGC CTCACACTTC CCGCTCGGAT TTGTGGCAAG AGTATTCGAC
    ATGCTGTTTC TTCAGGGGTC AGAGGTCATC TTCAAAGTGG CCTTGAGTCT TCTCGGGAGC
    CACAAACCTC TGATTCTCCA ACACGACAAT CTTGAGGCAA TCGTGGAGTT CATCAAAACA
    ACCTTACCCA ACCTCGGCCT CGTGCAAATG GAGAAAACTA TCAATCAGGT GTCAGAGATG
    GATATCAGTA AGCAGCTGCA GGCCTATGAA GTGGAATATC ATGTTCTTCA GGATGAGCTT
    TTGGATGGTC CCTCCACACT TAGCCATTCC CAGCGAGCGG CCCAGTTGGA GAAGACCAAT
    GGAAGTCTCC GACAACAAAA CTTAGACCTC CTAGAGGAGC TACAGGTTGC CCACGCTAAA
    ATCAGATGTT TAGAGACTCG TGTGGACGGC CTTGTGAACC GAGAGGCTGA GCTGCAATCA
    CAAGTAAAGA CTCTCCAGCA GGAACAAACA GATCTCCAGC AGACCGTATG CAAACTCAGA
    GACCTTCTCA GCAGTCTGGG CATAAGCAAC CCCTGCCCAG ACCTCCAAAT CCTCACAAAC
    AACAACCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05631
    Clone ID Related Accession (Same CDS sequence) XM_684525.9 , XM_684525.8 , XM_021476251.1
    Accession Version XM_684525.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product TBC1 domain family member 1 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_684525.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT GATGTTGCGG CCCTTTCCAG TCCTGGATGT
    GTTATTGGTG ATGACAGTCC ATTGCGCAAC CGGAGACACT CATGGAGACA ACAGATCTTC
    TTAAGAGTCG CTACACCACA GAAGGGCTCG GACGCCAAAG AATCCACGTT AGAGGTTGGC
    AGCAGAATGG CACTGGGAGG TGGAGTTGGT GGCGGAGACA AAACACTTGG GGCGGTGCCA
    GAGGAGAGGA GAAAGAGGAG CGGGGCGGAG CTTAGGAAGT TATGGAGGAA AGCCATTCTT
    CAACAGATAC TCCTACTGCG GATGGAGAAA GAGAATCAGA AACTACAAGC GTCAGAGAGT
    GATCTGCAGT GCAAGCGACA GAAGCTGGAT TATGAAGAGA TCACTCCATG CCTGAAAGAT
    GTCACTCTGG TCTGGGAGAG GATGCTCGGC ACGGCTGGAA GAGCAAAGGT CAGATTTGAC
    ATGGATGAGA TCCACGGTGC TGTGGGACAA GGCGTTCCTC GTCAGCATCG AGGAGAGATC
    TGGAAGTTTC TCTCTGAGCA GTATTTGCTG AGGCAGGAGG TGCCCAGTGC CAAACCGCCA
    AACAATGACA CTCCTTACAA AGAGCTACTG AAGCAGCTCA CATCCCAGCA GCATGCCATC
    CTCATTGACT TGGGTCGTAC ATTTCCCACT CACCCGTATT TCAGCGCTCA GCTTGGTGCA
    GGCCAGCTGT CCCTCTATAA CCTACTGAAG GCATATTCTC TGCTGGACCC GGAGGTCGGT
    TACTGCCAGG GCCTGAGCTT TGTGGCTGGT GTTTTGCTGC TCCACATGAG CGAGGAAGAC
    GCGTTTCACA TGCTCAAATT CCTCATGTAC GACATGGGGC TCAGGAAACA GTACAGGCCT
    GATATGATCA TACTGCAGAT TCAGATGTAT CAGCTGTCTC GTCTGTTGCA TGACTACCAC
    AGGGAGCTCT ACTCTCATCT GGAGCTTTAT GAGATCGGCC CCAGTCTCTA CGCCGCTCCA
    TGGTTCCTCA CCGCCTTTGC CTCACACTTC CCGCTCGGAT TTGTGGCAAG AGTATTCGAC
    ATGCTGTTTC TTCAGGGGTC AGAGGTCATC TTCAAAGTGG CCTTGAGTCT TCTCGGGAGC
    CACAAACCTC TGATTCTCCA ACACGACAAT CTTGAGGCAA TCGTGGAGTT CATCAAAACA
    ACCTTACCCA ACCTCGGCCT CGTGCAAATG GAGAAAACTA TCAATCAGGT GTCAGAGATG
    GATATCAGTA AGCAGCTGCA GGCCTATGAA GTGGAATATC ATGTTCTTCA GGATGAGCTT
    TTGGATGGTC CCTCCACACT TAGCCATTCC CAGCGAGCGG CCCAGTTGGA GAAGACCAAT
    GGAAGTCTCC GACAACAAAA CTTAGACCTC CTAGAGGAGC TACAGGTTGC CCACGCTAAA
    ATCAGATGTT TAGAGACTCG TGTGGACGGC CTTGTGAACC GAGAGGCTGA GCTGCAATCA
    CAAGTAAAGA CTCTCCAGCA GGAACAAACA GATCTCCAGC AGACCGTATG CAAACTCAGA
    GACCTTCTCA GCAGTCTGGG CATAAGCAAC CCCTGCCCAG ACCTCCAAAT CCTCACAAAC
    AACAACCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_689617.6
    CDS234..3845
    Translation

    Target ORF information:

    RefSeq Version XM_684525.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_684525.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT GATGTTGCGG CCCTTTCCAG TCCTGGATGT
    GTTATTGGTG ATGACAGTCC ATTGCGCAAC CGGAGACACT CATGGAGACA ACAGATCTTC
    TTAAGAGTCG CTACACCACA GAAGGGCTCG GACGCCAAAG AATCCACGTT AGAGGTTGGC
    AGCAGAATGG CACTGGGAGG TGGAGTTGGT GGCGGAGACA AAACACTTGG GGCGGTGCCA
    GAGGAGAGGA GAAAGAGGAG CGGGGCGGAG CTTAGGAAGT TATGGAGGAA AGCCATTCTT
    CAACAGATAC TCCTACTGCG GATGGAGAAA GAGAATCAGA AACTACAAGC GTCAGAGAGT
    GATCTGCAGT GCAAGCGACA GAAGCTGGAT TATGAAGAGA TCACTCCATG CCTGAAAGAT
    GTCACTCTGG TCTGGGAGAG GATGCTCGGC ACGGCTGGAA GAGCAAAGGT CAGATTTGAC
    ATGGATGAGA TCCACGGTGC TGTGGGACAA GGCGTTCCTC GTCAGCATCG AGGAGAGATC
    TGGAAGTTTC TCTCTGAGCA GTATTTGCTG AGGCAGGAGG TGCCCAGTGC CAAACCGCCA
    AACAATGACA CTCCTTACAA AGAGCTACTG AAGCAGCTCA CATCCCAGCA GCATGCCATC
    CTCATTGACT TGGGTCGTAC ATTTCCCACT CACCCGTATT TCAGCGCTCA GCTTGGTGCA
    GGCCAGCTGT CCCTCTATAA CCTACTGAAG GCATATTCTC TGCTGGACCC GGAGGTCGGT
    TACTGCCAGG GCCTGAGCTT TGTGGCTGGT GTTTTGCTGC TCCACATGAG CGAGGAAGAC
    GCGTTTCACA TGCTCAAATT CCTCATGTAC GACATGGGGC TCAGGAAACA GTACAGGCCT
    GATATGATCA TACTGCAGAT TCAGATGTAT CAGCTGTCTC GTCTGTTGCA TGACTACCAC
    AGGGAGCTCT ACTCTCATCT GGAGCTTTAT GAGATCGGCC CCAGTCTCTA CGCCGCTCCA
    TGGTTCCTCA CCGCCTTTGC CTCACACTTC CCGCTCGGAT TTGTGGCAAG AGTATTCGAC
    ATGCTGTTTC TTCAGGGGTC AGAGGTCATC TTCAAAGTGG CCTTGAGTCT TCTCGGGAGC
    CACAAACCTC TGATTCTCCA ACACGACAAT CTTGAGGCAA TCGTGGAGTT CATCAAAACA
    ACCTTACCCA ACCTCGGCCT CGTGCAAATG GAGAAAACTA TCAATCAGGT GTCAGAGATG
    GATATCAGTA AGCAGCTGCA GGCCTATGAA GTGGAATATC ATGTTCTTCA GGATGAGCTT
    TTGGATGGTC CCTCCACACT TAGCCATTCC CAGCGAGCGG CCCAGTTGGA GAAGACCAAT
    GGAAGTCTCC GACAACAAAA CTTAGACCTC CTAGAGGAGC TACAGGTTGC CCACGCTAAA
    ATCAGATGTT TAGAGACTCG TGTGGACGGC CTTGTGAACC GAGAGGCTGA GCTGCAATCA
    CAAGTAAAGA CTCTCCAGCA GGAACAAACA GATCTCCAGC AGACCGTATG CAAACTCAGA
    GACCTTCTCA GCAGTCTGGG CATAAGCAAC CCCTGCCCAG ACCTCCAAAT CCTCACAAAC
    AACAACCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05631
    Clone ID Related Accession (Same CDS sequence) XM_684525.9 , XM_684525.8 , XM_021476251.1
    Accession Version XM_021476251.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3612bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT GATGTTGCGG CCCTTTCCAG TCCTGGATGT
    GTTATTGGTG ATGACAGTCC ATTGCGCAAC CGGAGACACT CATGGAGACA ACAGATCTTC
    TTAAGAGTCG CTACACCACA GAAGGGCTCG GACGCCAAAG AATCCACGTT AGAGGTTGGC
    AGCAGAATGG CACTGGGAGG TGGAGTTGGT GGCGGAGACA AAACACTTGG GGCGGTGCCA
    GAGGAGAGGA GAAAGAGGAG CGGGGCGGAG CTTAGGAAGT TATGGAGGAA AGCCATTCTT
    CAACAGATAC TCCTACTGCG GATGGAGAAA GAGAATCAGA AACTACAAGC GTCAGAGAGT
    GATCTGCAGT GCAAGCGACA GAAGCTGGAT TATGAAGAGA TCACTCCATG CCTGAAAGAT
    GTCACTCTGG TCTGGGAGAG GATGCTCGGC ACGGCTGGAA GAGCAAAGGT CAGATTTGAC
    ATGGATGAGA TCCACGGTGC TGTGGGACAA GGCGTTCCTC GTCAGCATCG AGGAGAGATC
    TGGAAGTTTC TCTCTGAGCA GTATTTGCTG AGGCAGGAGG TGCCCAGTGC CAAACCGCCA
    AACAATGACA CTCCTTACAA AGAGCTACTG AAGCAGCTCA CATCCCAGCA GCATGCCATC
    CTCATTGACT TGGGTCGTAC ATTTCCCACT CACCCGTATT TCAGCGCTCA GCTTGGTGCA
    GGCCAGCTGT CCCTCTATAA CCTACTGAAG GCATATTCTC TGCTGGACCC GGAGGTCGGT
    TACTGCCAGG GCCTGAGCTT TGTGGCTGGT GTTTTGCTGC TCCACATGAG CGAGGAAGAC
    GCGTTTCACA TGCTCAAATT CCTCATGTAC GACATGGGGC TCAGGAAACA GTACAGGCCT
    GATATGATCA TACTGCAGAT TCAGATGTAT CAGCTGTCTC GTCTGTTGCA TGACTACCAC
    AGGGAGCTCT ACTCTCATCT GGAGCTTTAT GAGATCGGCC CCAGTCTCTA CGCCGCTCCA
    TGGTTCCTCA CCGCCTTTGC CTCACACTTC CCGCTCGGAT TTGTGGCAAG AGTATTCGAC
    ATGCTGTTTC TTCAGGGGTC AGAGGTCATC TTCAAAGTGG CCTTGAGTCT TCTCGGGAGC
    CACAAACCTC TGATTCTCCA ACACGACAAT CTTGAGGCAA TCGTGGAGTT CATCAAAACA
    ACCTTACCCA ACCTCGGCCT CGTGCAAATG GAGAAAACTA TCAATCAGGT GTCAGAGATG
    GATATCAGTA AGCAGCTGCA GGCCTATGAA GTGGAATATC ATGTTCTTCA GGATGAGCTT
    TTGGATGGTC CCTCCACACT TAGCCATTCC CAGCGAGCGG CCCAGTTGGA GAAGACCAAT
    GGAAGTCTCC GACAACAAAA CTTAGACCTC CTAGAGGAGC TACAGGTTGC CCACGCTAAA
    ATCAGATGTT TAGAGACTCG TGTGGACGGC CTTGTGAACC GAGAGGCTGA GCTGCAATCA
    CAAGTAAAGA CTCTCCAGCA GGAACAAACA GATCTCCAGC AGACCGTATG CAAACTCAGA
    GACCTTCTCA GCAGTCTGGG CATAAGCAAC CCCTGCCCAG ACCTCCAAAT CCTCACAAAC
    AACAACCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331926.1
    CDS252..3863
    Translation

    Target ORF information:

    RefSeq Version XM_021476251.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476251.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGAGGGCA TTTGTTTCGA GGTGAAACCC AGAGATGACT CAATAACAGC TCATCCCCTC 
    TGTGACCTTA CACAAACATT TCCTGGATCA GAGGAGAAGA GGAAGAAAGA GGATGGGGAA
    GAGGTGATCC AGTACAGACC GACTCTGGTT GGATCTTTGG CCATCCATCC ACAGACCACC
    ATGGCCATAC TCCCCTGGGT GGTGGCTGAA ATACGGAGGC CCGGGGTCAG GGAGAAGAAC
    AGAAACCTTG AGTTCAATGG CAATTACTCT CCCGAGATTC GAGATCAGCC AGTGGTGCTT
    CAGATTTCAG CCAGTTCCGT GTGCTGCATT CTGGACACGG CAGGCCCAGG GAAGCCATGG
    AACCCGTTGC AACATATGGT TCTGTTTGAT CATAGGCCTC ATCATGTCAC CAAACTCATT
    CACAACAGCC AAGAGCCCAG CTATTTTGGC TGTCTGCTCA GAGAGGAGAA AAAAGCAGCC
    TGCTATGTAT TCCGCTGCCA GGACCAGCTG AAGGTTCCAG AGATCATCAG CACTCTCAGA
    CAGGCTGGCA AAAGCTCTGC CCGGCAAGAA GACACTCCAT CACTGCCTAG CAACGGTCAA
    TCAGATGCCT CCAGCTCCAC GGCAACAGCA GCTGATGCAG CCACAGCCTT CGCTAAAAAG
    TTTGAGGTGT TGTTCTGCGG GCGAGTGGTG GTGGCCCATA AGAAAGCACC TCCTGCACTA
    ATAGATGAGT GTATAGAGAA GTTTGGACGT GTCAGTGTTA CAGGCTCACT TGCAGCGGGC
    ATCAGGCGGG CGTTTTCACT CAATCAGGTG GTGAATGGAA CAAATGAGGG TGATGGAGGA
    GGAAAGCGAC CTTTGTTTTT TAAAAGAGAT CAAAGTTTTC CATTTCTGCA GGCATTGGAT
    GAGAACGGCC TCTCACCGGA GCTCCAGCGA CGGGCAGCAG ACGGAGCCAC TGGCGTTCAA
    CCTACCAGTC TACAGGAGAA CAGAACCATG CTGTTTACAA TTGGGCGATC TCAGATCTTT
    CTCGTGAGTC CTGACACTAA GAAAGTGGCC ATAGAGAAGA GCTTCAGAGA GATTTCCTTC
    TGTTCTCAGG GAATTCGTCA CGTGGACCAC TTCGGCTTCA TATGCAGGGA GTCGGTTGAA
    TGCGGAGGCT GCCAGTTTGT TTGCTATGTT TTCCAGTGTG CAAACGAGGC TCTGGTGGAT
    GAGATCATGT TGACTTTGAA GCAGGCGTTC TCTGTGGCCG CTCTCCAGCA GAACAGCAGG
    ACCCAGAGTC AGCAGTGTGA CGGCTGTCCC CTGCAGCAGC TGCACAAACT CTGTGACAGA
    ATAGAGGGTC TTCATCCATC CAAAGCTAAG CTGGAACTGC AGAAGCATTT GGCAGATCTG
    GATAATCAGG AGCAGGCAGC TGTGTTTGAG AGCACCATGA AAGCCAGGCC TAAATCGGAT
    CAGGAGGAAA ATGAGTTGAT TTTCTCGTGT CTAAGACGGA TGTACGAGGA CAGACAAAAG
    ACACACACAC ACAACGGGCA AACAAAAAAG GATGTTGTTG TGAATGCTGA AAGCAGCGTG
    AGTTCTAGCC GTGTGCGTCT GGAGGAGTTC AAGTCTAGAG CTAAAAGATC CCTCACTGAG
    TCACTGGAGG GGATCTGGAA GGGTGGCAAT AAGTCAAAAT CCCAGAGGGA AAACAGTGAA
    GGAGAGAGCA ACTCCTCCAG TTCTACCACA CTGAGCAGCA CACATCAGGA AACATCATTG
    CAGGAGCCAC TCTGCTCTCT GCTTCTCTCC AGTCCGCTGC GCTCCCACAA TTCCACAGGG
    AATCTGAAGC ATCTTGAAGG CTCTGACAGG GGTCAGGAGG ATCCTGAGAG TCCGCCTCAG
    GGTTTCAGGA GACGCGCAAG CACTATCAGT CACTCTCCCA TGCTGTCGTC CTCTGAGCCG
    AGCCTCCCTC CTCTACAGCC CACTGCTGCC AACAAACCCA AACTGGTCCG ACACTACTCC
    GTCAGCACAG ACTCCCCACA CCAGAGCAAT GATGTTGCGG CCCTTTCCAG TCCTGGATGT
    GTTATTGGTG ATGACAGTCC ATTGCGCAAC CGGAGACACT CATGGAGACA ACAGATCTTC
    TTAAGAGTCG CTACACCACA GAAGGGCTCG GACGCCAAAG AATCCACGTT AGAGGTTGGC
    AGCAGAATGG CACTGGGAGG TGGAGTTGGT GGCGGAGACA AAACACTTGG GGCGGTGCCA
    GAGGAGAGGA GAAAGAGGAG CGGGGCGGAG CTTAGGAAGT TATGGAGGAA AGCCATTCTT
    CAACAGATAC TCCTACTGCG GATGGAGAAA GAGAATCAGA AACTACAAGC GTCAGAGAGT
    GATCTGCAGT GCAAGCGACA GAAGCTGGAT TATGAAGAGA TCACTCCATG CCTGAAAGAT
    GTCACTCTGG TCTGGGAGAG GATGCTCGGC ACGGCTGGAA GAGCAAAGGT CAGATTTGAC
    ATGGATGAGA TCCACGGTGC TGTGGGACAA GGCGTTCCTC GTCAGCATCG AGGAGAGATC
    TGGAAGTTTC TCTCTGAGCA GTATTTGCTG AGGCAGGAGG TGCCCAGTGC CAAACCGCCA
    AACAATGACA CTCCTTACAA AGAGCTACTG AAGCAGCTCA CATCCCAGCA GCATGCCATC
    CTCATTGACT TGGGTCGTAC ATTTCCCACT CACCCGTATT TCAGCGCTCA GCTTGGTGCA
    GGCCAGCTGT CCCTCTATAA CCTACTGAAG GCATATTCTC TGCTGGACCC GGAGGTCGGT
    TACTGCCAGG GCCTGAGCTT TGTGGCTGGT GTTTTGCTGC TCCACATGAG CGAGGAAGAC
    GCGTTTCACA TGCTCAAATT CCTCATGTAC GACATGGGGC TCAGGAAACA GTACAGGCCT
    GATATGATCA TACTGCAGAT TCAGATGTAT CAGCTGTCTC GTCTGTTGCA TGACTACCAC
    AGGGAGCTCT ACTCTCATCT GGAGCTTTAT GAGATCGGCC CCAGTCTCTA CGCCGCTCCA
    TGGTTCCTCA CCGCCTTTGC CTCACACTTC CCGCTCGGAT TTGTGGCAAG AGTATTCGAC
    ATGCTGTTTC TTCAGGGGTC AGAGGTCATC TTCAAAGTGG CCTTGAGTCT TCTCGGGAGC
    CACAAACCTC TGATTCTCCA ACACGACAAT CTTGAGGCAA TCGTGGAGTT CATCAAAACA
    ACCTTACCCA ACCTCGGCCT CGTGCAAATG GAGAAAACTA TCAATCAGGT GTCAGAGATG
    GATATCAGTA AGCAGCTGCA GGCCTATGAA GTGGAATATC ATGTTCTTCA GGATGAGCTT
    TTGGATGGTC CCTCCACACT TAGCCATTCC CAGCGAGCGG CCCAGTTGGA GAAGACCAAT
    GGAAGTCTCC GACAACAAAA CTTAGACCTC CTAGAGGAGC TACAGGTTGC CCACGCTAAA
    ATCAGATGTT TAGAGACTCG TGTGGACGGC CTTGTGAACC GAGAGGCTGA GCTGCAATCA
    CAAGTAAAGA CTCTCCAGCA GGAACAAACA GATCTCCAGC AGACCGTATG CAAACTCAGA
    GACCTTCTCA GCAGTCTGGG CATAAGCAAC CCCTGCCCAG ACCTCCAAAT CCTCACAAAC
    AACAACCAAT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05632
    Clone ID Related Accession (Same CDS sequence) XM_005168371.3 , XM_005168371.4 , XM_021476253.1
    Accession Version XM_005168371.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product TBC1 domain family member 1 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005168371.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTGAAG CGATGAAAGC GCGCTTTCAC ATCTTCCATA AACTGCGGAG GGATGTGAAA 
    AGCGAGCAGG AGGCAGTTCC AGAGATCATC AGCACTCTCA GACAGGCTGG CAAAAGCTCT
    GCCCGGCAAG AAGACACTCC ATCACTGCCT AGCAACGGTC AATCAGATGC CTCCAGCTCC
    ACGGCAACAG CAGCTGATGC AGCCACAGCC TTCGCTAAAA AGTTTGAGGT GTTGTTCTGC
    GGGCGAGTGG TGGTGGCCCA TAAGAAAGCA CCTCCTGCAC TAATAGATGA GTGTATAGAG
    AAGTTTGGAC GTGTCAGTGT TACAGGCTCA CTTGCAGCGG GCATCAGGCG GGCGTTTTCA
    CTCAATCAGG TGGTGAATGG AACAAATGAG GGTGATGGAG GAGGAAAGCG ACCTTTGTTT
    TTTAAAAGAG ATCAAAGTTT TCCATTTCTG CAGGCATTGG ATGAGAACGG CCTCTCACCG
    GAGCTCCAGC GACGGGCAGC AGACGGAGCC ACTGGCGTTC AACCTACCAG TCTACAGGAG
    AACAGAACCA TGCTGTTTAC AATTGGGCGA TCTCAGATCT TTCTCGTGAG TCCTGACACT
    AAGAAAGTGG CCATAGAGAA GAGCTTCAGA GAGATTTCCT TCTGTTCTCA GGGAATTCGT
    CACGTGGACC ACTTCGGCTT CATATGCAGG GAGTCGGTTG AATGCGGAGG CTGCCAGTTT
    GTTTGCTATG TTTTCCAGTG TGCAAACGAG GCTCTGGTGG ATGAGATCAT GTTGACTTTG
    AAGCAGGCGT TCTCTGTGGC CGCTCTCCAG CAGAACAGCA GGACCCAGAG TCAGCAGTGT
    GACGGCTGTC CCCTGCAGCA GCTGCACAAA CTCTGTGACA GAATAGAGGG TCTTCATCCA
    TCCAAAGCTA AGCTGGAACT GCAGAAGCAT TTGGCAGATC TGGATAATCA GGAGCAGGCA
    GCTGTGTTTG AGAGCACCAT GAAAGCCAGG CCTAAATCGG ATCAGGAGGA AAATGAGTTG
    ATTTTCTCGT GTCTAAGACG GATGTACGAG GACAGACAAA AGACACACAC ACACAACGGG
    CAAACAAAAA AGGATGTTGT TGTGAATGCT GAAAGCAGCG TGAGTTCTAG CCGTGTGCGT
    CTGGAGGAGT TCAAGTCTAG AGCTAAAAGA TCCCTCACTG AGTCACTGGA GGGGATCTGG
    AAGGGTGGCA ATAAGTCAAA ATCCCAGAGG GAAAACAGTG AAGGAGAGAG CAACTCCTCC
    AGTTCTACCA CACTGAGCAG CACACATCAG GAAACATCAT TGCAGGAGCC ACTCTGCTCT
    CTGCTTCTCT CCAGTCCGCT GCGCTCCCAC AATTCCACAG GGAATCTGAA GCATCTTGAA
    GGCTCTGACA GGGGTCAGGA GGATCCTGAG AGTCCGCCTC AGGGTTTCAG GAGACGCGCA
    AGCACTATCA GTCACTCTCC CATGCTGTCG TCCTCTGAGC CGAGCCTCCC TCCTCTACAG
    CCCACTGCTG CCAACAAACC CAAACTGGTC CGACACTACT CCGTCAGCAC AGACTCCCCA
    CACCAGAGCA ATCCTTCCTC CTCTCCGGCT TCTTCCTCTC CTGTGTGTCA GCGGCCGCTG
    AGGCCTCGTT CTTTTGCTCT TGATCATTCC ACACGGCCTC TCAAGCGCAG TCCATCAGGG
    CTGAGTGGCT TTAGAGCGAG ATTACACTCG TCCTCCTCTG TTCCAAATTT CCTCAAATTT
    CTGGCCCCTG TAGTGGAAAG TGATGATACC TGTGACTCTC AGAGGAAGAG TGATGTTGCG
    GCCCTTTCCA GTCCTGGATG TGTTATTGGT GATGACAGTC CATTGCGCAA CCGGAGACAC
    TCATGGAGAC AACAGATCTT CTTAAGAGTC GCTACACCAC AGAAGGGCTC GGACGCCAAA
    GAATCCACGT TAGAGGTTGG CAGCAGAATG GCACTGGGAG GTGGAGTTGG TGGCGGAGAC
    AAAACACTTG GGGCGGTGCC AGAGGAGAGG AGAAAGAGGA GCGGGGCGGA GCTTAGGAAG
    TTATGGAGGA AAGCCATTCT TCAACAGATA CTCCTACTGC GGATGGAGAA AGAGAATCAG
    AAACTACAAG CGTCAGAGAG TGATCTGCAG TGCAAGCGAC AGAAGCTGGA TTATGAAGAG
    ATCACTCCAT GCCTGAAAGA TGTCACTCTG GTCTGGGAGA GGATGCTCGG CACGGCTGGA
    AGAGCAAAGG TCAGATTTGA CATGGATGAG ATCCACGGTG CTGTGGGACA AGGCGTTCCT
    CGTCAGCATC GAGGAGAGAT CTGGAAGTTT CTCTCTGAGC AGTATTTGCT GAGGCAGGAG
    GTGCCCAGTG CCAAACCGCC AAACAATGAC ACTCCTTACA AAGAGCTACT GAAGCAGCTC
    ACATCCCAGC AGCATGCCAT CCTCATTGAC TTGGGTCGTA CATTTCCCAC TCACCCGTAT
    TTCAGCGCTC AGCTTGGTGC AGGCCAGCTG TCCCTCTATA ACCTACTGAA GGCATATTCT
    CTGCTGGACC CGGAGGTCGG TTACTGCCAG GGCCTGAGCT TTGTGGCTGG TGTTTTGCTG
    CTCCACATGA GCGAGGAAGA CGCGTTTCAC ATGCTCAAAT TCCTCATGTA CGACATGGGG
    CTCAGGAAAC AGTACAGGCC TGATATGATC ATACTGCAGA TTCAGATGTA TCAGCTGTCT
    CGTCTGTTGC ATGACTACCA CAGGGAGCTC TACTCTCATC TGGAGCTTTA TGAGATCGGC
    CCCAGTCTCT ACGCCGCTCC ATGGTTCCTC ACCGCCTTTG CCTCACACTT CCCGCTCGGA
    TTTGTGGCAA GAGTATTCGA CATGCTGTTT CTTCAGGGGT CAGAGGTCAT CTTCAAAGTG
    GCCTTGAGTC TTCTCGGGAG CCACAAACCT CTGATTCTCC AACACGACAA TCTTGAGGCA
    ATCGTGGAGT TCATCAAAAC AACCTTACCC AACCTCGGCC TCGTGCAAAT GGAGAAAACT
    ATCAATCAGG TGTCAGAGAT GGATATCAGT AAGCAGCTGC AGGCCTATGA AGTGGAATAT
    CATGTTCTTC AGGATGAGCT TTTGGATGGT CCCTCCACAC TTAGCCATTC CCAGCGAGCG
    GCCCAGTTGG AGAAGACCAA TGGAAGTCTC CGACAACAAA ACTTAGACCT CCTAGAGGAG
    CTACAGGTTG CCCACGCTAA AATCAGATGT TTAGAGACTC GTGTGGACGG CCTTGTGAAC
    CGAGAGGCTG AGCTGCAATC ACAAGTAAAG ACTCTCCAGC AGGAACAAAC AGATCTCCAG
    CAGACCGTAT GCAAACTCAG AGACCTTCTC AGCAGTCTGG GCATAAGCAA CCCCTGCCCA
    GACCTCCAAA TCCTCACAAA CAACAACCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168428.1
    CDS154..3546
    Translation

    Target ORF information:

    RefSeq Version XM_005168371.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168371.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTGAAG CGATGAAAGC GCGCTTTCAC ATCTTCCATA AACTGCGGAG GGATGTGAAA 
    AGCGAGCAGG AGGCAGTTCC AGAGATCATC AGCACTCTCA GACAGGCTGG CAAAAGCTCT
    GCCCGGCAAG AAGACACTCC ATCACTGCCT AGCAACGGTC AATCAGATGC CTCCAGCTCC
    ACGGCAACAG CAGCTGATGC AGCCACAGCC TTCGCTAAAA AGTTTGAGGT GTTGTTCTGC
    GGGCGAGTGG TGGTGGCCCA TAAGAAAGCA CCTCCTGCAC TAATAGATGA GTGTATAGAG
    AAGTTTGGAC GTGTCAGTGT TACAGGCTCA CTTGCAGCGG GCATCAGGCG GGCGTTTTCA
    CTCAATCAGG TGGTGAATGG AACAAATGAG GGTGATGGAG GAGGAAAGCG ACCTTTGTTT
    TTTAAAAGAG ATCAAAGTTT TCCATTTCTG CAGGCATTGG ATGAGAACGG CCTCTCACCG
    GAGCTCCAGC GACGGGCAGC AGACGGAGCC ACTGGCGTTC AACCTACCAG TCTACAGGAG
    AACAGAACCA TGCTGTTTAC AATTGGGCGA TCTCAGATCT TTCTCGTGAG TCCTGACACT
    AAGAAAGTGG CCATAGAGAA GAGCTTCAGA GAGATTTCCT TCTGTTCTCA GGGAATTCGT
    CACGTGGACC ACTTCGGCTT CATATGCAGG GAGTCGGTTG AATGCGGAGG CTGCCAGTTT
    GTTTGCTATG TTTTCCAGTG TGCAAACGAG GCTCTGGTGG ATGAGATCAT GTTGACTTTG
    AAGCAGGCGT TCTCTGTGGC CGCTCTCCAG CAGAACAGCA GGACCCAGAG TCAGCAGTGT
    GACGGCTGTC CCCTGCAGCA GCTGCACAAA CTCTGTGACA GAATAGAGGG TCTTCATCCA
    TCCAAAGCTA AGCTGGAACT GCAGAAGCAT TTGGCAGATC TGGATAATCA GGAGCAGGCA
    GCTGTGTTTG AGAGCACCAT GAAAGCCAGG CCTAAATCGG ATCAGGAGGA AAATGAGTTG
    ATTTTCTCGT GTCTAAGACG GATGTACGAG GACAGACAAA AGACACACAC ACACAACGGG
    CAAACAAAAA AGGATGTTGT TGTGAATGCT GAAAGCAGCG TGAGTTCTAG CCGTGTGCGT
    CTGGAGGAGT TCAAGTCTAG AGCTAAAAGA TCCCTCACTG AGTCACTGGA GGGGATCTGG
    AAGGGTGGCA ATAAGTCAAA ATCCCAGAGG GAAAACAGTG AAGGAGAGAG CAACTCCTCC
    AGTTCTACCA CACTGAGCAG CACACATCAG GAAACATCAT TGCAGGAGCC ACTCTGCTCT
    CTGCTTCTCT CCAGTCCGCT GCGCTCCCAC AATTCCACAG GGAATCTGAA GCATCTTGAA
    GGCTCTGACA GGGGTCAGGA GGATCCTGAG AGTCCGCCTC AGGGTTTCAG GAGACGCGCA
    AGCACTATCA GTCACTCTCC CATGCTGTCG TCCTCTGAGC CGAGCCTCCC TCCTCTACAG
    CCCACTGCTG CCAACAAACC CAAACTGGTC CGACACTACT CCGTCAGCAC AGACTCCCCA
    CACCAGAGCA ATCCTTCCTC CTCTCCGGCT TCTTCCTCTC CTGTGTGTCA GCGGCCGCTG
    AGGCCTCGTT CTTTTGCTCT TGATCATTCC ACACGGCCTC TCAAGCGCAG TCCATCAGGG
    CTGAGTGGCT TTAGAGCGAG ATTACACTCG TCCTCCTCTG TTCCAAATTT CCTCAAATTT
    CTGGCCCCTG TAGTGGAAAG TGATGATACC TGTGACTCTC AGAGGAAGAG TGATGTTGCG
    GCCCTTTCCA GTCCTGGATG TGTTATTGGT GATGACAGTC CATTGCGCAA CCGGAGACAC
    TCATGGAGAC AACAGATCTT CTTAAGAGTC GCTACACCAC AGAAGGGCTC GGACGCCAAA
    GAATCCACGT TAGAGGTTGG CAGCAGAATG GCACTGGGAG GTGGAGTTGG TGGCGGAGAC
    AAAACACTTG GGGCGGTGCC AGAGGAGAGG AGAAAGAGGA GCGGGGCGGA GCTTAGGAAG
    TTATGGAGGA AAGCCATTCT TCAACAGATA CTCCTACTGC GGATGGAGAA AGAGAATCAG
    AAACTACAAG CGTCAGAGAG TGATCTGCAG TGCAAGCGAC AGAAGCTGGA TTATGAAGAG
    ATCACTCCAT GCCTGAAAGA TGTCACTCTG GTCTGGGAGA GGATGCTCGG CACGGCTGGA
    AGAGCAAAGG TCAGATTTGA CATGGATGAG ATCCACGGTG CTGTGGGACA AGGCGTTCCT
    CGTCAGCATC GAGGAGAGAT CTGGAAGTTT CTCTCTGAGC AGTATTTGCT GAGGCAGGAG
    GTGCCCAGTG CCAAACCGCC AAACAATGAC ACTCCTTACA AAGAGCTACT GAAGCAGCTC
    ACATCCCAGC AGCATGCCAT CCTCATTGAC TTGGGTCGTA CATTTCCCAC TCACCCGTAT
    TTCAGCGCTC AGCTTGGTGC AGGCCAGCTG TCCCTCTATA ACCTACTGAA GGCATATTCT
    CTGCTGGACC CGGAGGTCGG TTACTGCCAG GGCCTGAGCT TTGTGGCTGG TGTTTTGCTG
    CTCCACATGA GCGAGGAAGA CGCGTTTCAC ATGCTCAAAT TCCTCATGTA CGACATGGGG
    CTCAGGAAAC AGTACAGGCC TGATATGATC ATACTGCAGA TTCAGATGTA TCAGCTGTCT
    CGTCTGTTGC ATGACTACCA CAGGGAGCTC TACTCTCATC TGGAGCTTTA TGAGATCGGC
    CCCAGTCTCT ACGCCGCTCC ATGGTTCCTC ACCGCCTTTG CCTCACACTT CCCGCTCGGA
    TTTGTGGCAA GAGTATTCGA CATGCTGTTT CTTCAGGGGT CAGAGGTCAT CTTCAAAGTG
    GCCTTGAGTC TTCTCGGGAG CCACAAACCT CTGATTCTCC AACACGACAA TCTTGAGGCA
    ATCGTGGAGT TCATCAAAAC AACCTTACCC AACCTCGGCC TCGTGCAAAT GGAGAAAACT
    ATCAATCAGG TGTCAGAGAT GGATATCAGT AAGCAGCTGC AGGCCTATGA AGTGGAATAT
    CATGTTCTTC AGGATGAGCT TTTGGATGGT CCCTCCACAC TTAGCCATTC CCAGCGAGCG
    GCCCAGTTGG AGAAGACCAA TGGAAGTCTC CGACAACAAA ACTTAGACCT CCTAGAGGAG
    CTACAGGTTG CCCACGCTAA AATCAGATGT TTAGAGACTC GTGTGGACGG CCTTGTGAAC
    CGAGAGGCTG AGCTGCAATC ACAAGTAAAG ACTCTCCAGC AGGAACAAAC AGATCTCCAG
    CAGACCGTAT GCAAACTCAG AGACCTTCTC AGCAGTCTGG GCATAAGCAA CCCCTGCCCA
    GACCTCCAAA TCCTCACAAA CAACAACCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05632
    Clone ID Related Accession (Same CDS sequence) XM_005168371.3 , XM_005168371.4 , XM_021476253.1
    Accession Version XM_005168371.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394473.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005168371.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTGAAG CGATGAAAGC GCGCTTTCAC ATCTTCCATA AACTGCGGAG GGATGTGAAA 
    AGCGAGCAGG AGGCAGTTCC AGAGATCATC AGCACTCTCA GACAGGCTGG CAAAAGCTCT
    GCCCGGCAAG AAGACACTCC ATCACTGCCT AGCAACGGTC AATCAGATGC CTCCAGCTCC
    ACGGCAACAG CAGCTGATGC AGCCACAGCC TTCGCTAAAA AGTTTGAGGT GTTGTTCTGC
    GGGCGAGTGG TGGTGGCCCA TAAGAAAGCA CCTCCTGCAC TAATAGATGA GTGTATAGAG
    AAGTTTGGAC GTGTCAGTGT TACAGGCTCA CTTGCAGCGG GCATCAGGCG GGCGTTTTCA
    CTCAATCAGG TGGTGAATGG AACAAATGAG GGTGATGGAG GAGGAAAGCG ACCTTTGTTT
    TTTAAAAGAG ATCAAAGTTT TCCATTTCTG CAGGCATTGG ATGAGAACGG CCTCTCACCG
    GAGCTCCAGC GACGGGCAGC AGACGGAGCC ACTGGCGTTC AACCTACCAG TCTACAGGAG
    AACAGAACCA TGCTGTTTAC AATTGGGCGA TCTCAGATCT TTCTCGTGAG TCCTGACACT
    AAGAAAGTGG CCATAGAGAA GAGCTTCAGA GAGATTTCCT TCTGTTCTCA GGGAATTCGT
    CACGTGGACC ACTTCGGCTT CATATGCAGG GAGTCGGTTG AATGCGGAGG CTGCCAGTTT
    GTTTGCTATG TTTTCCAGTG TGCAAACGAG GCTCTGGTGG ATGAGATCAT GTTGACTTTG
    AAGCAGGCGT TCTCTGTGGC CGCTCTCCAG CAGAACAGCA GGACCCAGAG TCAGCAGTGT
    GACGGCTGTC CCCTGCAGCA GCTGCACAAA CTCTGTGACA GAATAGAGGG TCTTCATCCA
    TCCAAAGCTA AGCTGGAACT GCAGAAGCAT TTGGCAGATC TGGATAATCA GGAGCAGGCA
    GCTGTGTTTG AGAGCACCAT GAAAGCCAGG CCTAAATCGG ATCAGGAGGA AAATGAGTTG
    ATTTTCTCGT GTCTAAGACG GATGTACGAG GACAGACAAA AGACACACAC ACACAACGGG
    CAAACAAAAA AGGATGTTGT TGTGAATGCT GAAAGCAGCG TGAGTTCTAG CCGTGTGCGT
    CTGGAGGAGT TCAAGTCTAG AGCTAAAAGA TCCCTCACTG AGTCACTGGA GGGGATCTGG
    AAGGGTGGCA ATAAGTCAAA ATCCCAGAGG GAAAACAGTG AAGGAGAGAG CAACTCCTCC
    AGTTCTACCA CACTGAGCAG CACACATCAG GAAACATCAT TGCAGGAGCC ACTCTGCTCT
    CTGCTTCTCT CCAGTCCGCT GCGCTCCCAC AATTCCACAG GGAATCTGAA GCATCTTGAA
    GGCTCTGACA GGGGTCAGGA GGATCCTGAG AGTCCGCCTC AGGGTTTCAG GAGACGCGCA
    AGCACTATCA GTCACTCTCC CATGCTGTCG TCCTCTGAGC CGAGCCTCCC TCCTCTACAG
    CCCACTGCTG CCAACAAACC CAAACTGGTC CGACACTACT CCGTCAGCAC AGACTCCCCA
    CACCAGAGCA ATCCTTCCTC CTCTCCGGCT TCTTCCTCTC CTGTGTGTCA GCGGCCGCTG
    AGGCCTCGTT CTTTTGCTCT TGATCATTCC ACACGGCCTC TCAAGCGCAG TCCATCAGGG
    CTGAGTGGCT TTAGAGCGAG ATTACACTCG TCCTCCTCTG TTCCAAATTT CCTCAAATTT
    CTGGCCCCTG TAGTGGAAAG TGATGATACC TGTGACTCTC AGAGGAAGAG TGATGTTGCG
    GCCCTTTCCA GTCCTGGATG TGTTATTGGT GATGACAGTC CATTGCGCAA CCGGAGACAC
    TCATGGAGAC AACAGATCTT CTTAAGAGTC GCTACACCAC AGAAGGGCTC GGACGCCAAA
    GAATCCACGT TAGAGGTTGG CAGCAGAATG GCACTGGGAG GTGGAGTTGG TGGCGGAGAC
    AAAACACTTG GGGCGGTGCC AGAGGAGAGG AGAAAGAGGA GCGGGGCGGA GCTTAGGAAG
    TTATGGAGGA AAGCCATTCT TCAACAGATA CTCCTACTGC GGATGGAGAA AGAGAATCAG
    AAACTACAAG CGTCAGAGAG TGATCTGCAG TGCAAGCGAC AGAAGCTGGA TTATGAAGAG
    ATCACTCCAT GCCTGAAAGA TGTCACTCTG GTCTGGGAGA GGATGCTCGG CACGGCTGGA
    AGAGCAAAGG TCAGATTTGA CATGGATGAG ATCCACGGTG CTGTGGGACA AGGCGTTCCT
    CGTCAGCATC GAGGAGAGAT CTGGAAGTTT CTCTCTGAGC AGTATTTGCT GAGGCAGGAG
    GTGCCCAGTG CCAAACCGCC AAACAATGAC ACTCCTTACA AAGAGCTACT GAAGCAGCTC
    ACATCCCAGC AGCATGCCAT CCTCATTGAC TTGGGTCGTA CATTTCCCAC TCACCCGTAT
    TTCAGCGCTC AGCTTGGTGC AGGCCAGCTG TCCCTCTATA ACCTACTGAA GGCATATTCT
    CTGCTGGACC CGGAGGTCGG TTACTGCCAG GGCCTGAGCT TTGTGGCTGG TGTTTTGCTG
    CTCCACATGA GCGAGGAAGA CGCGTTTCAC ATGCTCAAAT TCCTCATGTA CGACATGGGG
    CTCAGGAAAC AGTACAGGCC TGATATGATC ATACTGCAGA TTCAGATGTA TCAGCTGTCT
    CGTCTGTTGC ATGACTACCA CAGGGAGCTC TACTCTCATC TGGAGCTTTA TGAGATCGGC
    CCCAGTCTCT ACGCCGCTCC ATGGTTCCTC ACCGCCTTTG CCTCACACTT CCCGCTCGGA
    TTTGTGGCAA GAGTATTCGA CATGCTGTTT CTTCAGGGGT CAGAGGTCAT CTTCAAAGTG
    GCCTTGAGTC TTCTCGGGAG CCACAAACCT CTGATTCTCC AACACGACAA TCTTGAGGCA
    ATCGTGGAGT TCATCAAAAC AACCTTACCC AACCTCGGCC TCGTGCAAAT GGAGAAAACT
    ATCAATCAGG TGTCAGAGAT GGATATCAGT AAGCAGCTGC AGGCCTATGA AGTGGAATAT
    CATGTTCTTC AGGATGAGCT TTTGGATGGT CCCTCCACAC TTAGCCATTC CCAGCGAGCG
    GCCCAGTTGG AGAAGACCAA TGGAAGTCTC CGACAACAAA ACTTAGACCT CCTAGAGGAG
    CTACAGGTTG CCCACGCTAA AATCAGATGT TTAGAGACTC GTGTGGACGG CCTTGTGAAC
    CGAGAGGCTG AGCTGCAATC ACAAGTAAAG ACTCTCCAGC AGGAACAAAC AGATCTCCAG
    CAGACCGTAT GCAAACTCAG AGACCTTCTC AGCAGTCTGG GCATAAGCAA CCCCTGCCCA
    GACCTCCAAA TCCTCACAAA CAACAACCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005168428.1
    CDS161..3553
    Translation

    Target ORF information:

    RefSeq Version XM_005168371.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005168371.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTGAAG CGATGAAAGC GCGCTTTCAC ATCTTCCATA AACTGCGGAG GGATGTGAAA 
    AGCGAGCAGG AGGCAGTTCC AGAGATCATC AGCACTCTCA GACAGGCTGG CAAAAGCTCT
    GCCCGGCAAG AAGACACTCC ATCACTGCCT AGCAACGGTC AATCAGATGC CTCCAGCTCC
    ACGGCAACAG CAGCTGATGC AGCCACAGCC TTCGCTAAAA AGTTTGAGGT GTTGTTCTGC
    GGGCGAGTGG TGGTGGCCCA TAAGAAAGCA CCTCCTGCAC TAATAGATGA GTGTATAGAG
    AAGTTTGGAC GTGTCAGTGT TACAGGCTCA CTTGCAGCGG GCATCAGGCG GGCGTTTTCA
    CTCAATCAGG TGGTGAATGG AACAAATGAG GGTGATGGAG GAGGAAAGCG ACCTTTGTTT
    TTTAAAAGAG ATCAAAGTTT TCCATTTCTG CAGGCATTGG ATGAGAACGG CCTCTCACCG
    GAGCTCCAGC GACGGGCAGC AGACGGAGCC ACTGGCGTTC AACCTACCAG TCTACAGGAG
    AACAGAACCA TGCTGTTTAC AATTGGGCGA TCTCAGATCT TTCTCGTGAG TCCTGACACT
    AAGAAAGTGG CCATAGAGAA GAGCTTCAGA GAGATTTCCT TCTGTTCTCA GGGAATTCGT
    CACGTGGACC ACTTCGGCTT CATATGCAGG GAGTCGGTTG AATGCGGAGG CTGCCAGTTT
    GTTTGCTATG TTTTCCAGTG TGCAAACGAG GCTCTGGTGG ATGAGATCAT GTTGACTTTG
    AAGCAGGCGT TCTCTGTGGC CGCTCTCCAG CAGAACAGCA GGACCCAGAG TCAGCAGTGT
    GACGGCTGTC CCCTGCAGCA GCTGCACAAA CTCTGTGACA GAATAGAGGG TCTTCATCCA
    TCCAAAGCTA AGCTGGAACT GCAGAAGCAT TTGGCAGATC TGGATAATCA GGAGCAGGCA
    GCTGTGTTTG AGAGCACCAT GAAAGCCAGG CCTAAATCGG ATCAGGAGGA AAATGAGTTG
    ATTTTCTCGT GTCTAAGACG GATGTACGAG GACAGACAAA AGACACACAC ACACAACGGG
    CAAACAAAAA AGGATGTTGT TGTGAATGCT GAAAGCAGCG TGAGTTCTAG CCGTGTGCGT
    CTGGAGGAGT TCAAGTCTAG AGCTAAAAGA TCCCTCACTG AGTCACTGGA GGGGATCTGG
    AAGGGTGGCA ATAAGTCAAA ATCCCAGAGG GAAAACAGTG AAGGAGAGAG CAACTCCTCC
    AGTTCTACCA CACTGAGCAG CACACATCAG GAAACATCAT TGCAGGAGCC ACTCTGCTCT
    CTGCTTCTCT CCAGTCCGCT GCGCTCCCAC AATTCCACAG GGAATCTGAA GCATCTTGAA
    GGCTCTGACA GGGGTCAGGA GGATCCTGAG AGTCCGCCTC AGGGTTTCAG GAGACGCGCA
    AGCACTATCA GTCACTCTCC CATGCTGTCG TCCTCTGAGC CGAGCCTCCC TCCTCTACAG
    CCCACTGCTG CCAACAAACC CAAACTGGTC CGACACTACT CCGTCAGCAC AGACTCCCCA
    CACCAGAGCA ATCCTTCCTC CTCTCCGGCT TCTTCCTCTC CTGTGTGTCA GCGGCCGCTG
    AGGCCTCGTT CTTTTGCTCT TGATCATTCC ACACGGCCTC TCAAGCGCAG TCCATCAGGG
    CTGAGTGGCT TTAGAGCGAG ATTACACTCG TCCTCCTCTG TTCCAAATTT CCTCAAATTT
    CTGGCCCCTG TAGTGGAAAG TGATGATACC TGTGACTCTC AGAGGAAGAG TGATGTTGCG
    GCCCTTTCCA GTCCTGGATG TGTTATTGGT GATGACAGTC CATTGCGCAA CCGGAGACAC
    TCATGGAGAC AACAGATCTT CTTAAGAGTC GCTACACCAC AGAAGGGCTC GGACGCCAAA
    GAATCCACGT TAGAGGTTGG CAGCAGAATG GCACTGGGAG GTGGAGTTGG TGGCGGAGAC
    AAAACACTTG GGGCGGTGCC AGAGGAGAGG AGAAAGAGGA GCGGGGCGGA GCTTAGGAAG
    TTATGGAGGA AAGCCATTCT TCAACAGATA CTCCTACTGC GGATGGAGAA AGAGAATCAG
    AAACTACAAG CGTCAGAGAG TGATCTGCAG TGCAAGCGAC AGAAGCTGGA TTATGAAGAG
    ATCACTCCAT GCCTGAAAGA TGTCACTCTG GTCTGGGAGA GGATGCTCGG CACGGCTGGA
    AGAGCAAAGG TCAGATTTGA CATGGATGAG ATCCACGGTG CTGTGGGACA AGGCGTTCCT
    CGTCAGCATC GAGGAGAGAT CTGGAAGTTT CTCTCTGAGC AGTATTTGCT GAGGCAGGAG
    GTGCCCAGTG CCAAACCGCC AAACAATGAC ACTCCTTACA AAGAGCTACT GAAGCAGCTC
    ACATCCCAGC AGCATGCCAT CCTCATTGAC TTGGGTCGTA CATTTCCCAC TCACCCGTAT
    TTCAGCGCTC AGCTTGGTGC AGGCCAGCTG TCCCTCTATA ACCTACTGAA GGCATATTCT
    CTGCTGGACC CGGAGGTCGG TTACTGCCAG GGCCTGAGCT TTGTGGCTGG TGTTTTGCTG
    CTCCACATGA GCGAGGAAGA CGCGTTTCAC ATGCTCAAAT TCCTCATGTA CGACATGGGG
    CTCAGGAAAC AGTACAGGCC TGATATGATC ATACTGCAGA TTCAGATGTA TCAGCTGTCT
    CGTCTGTTGC ATGACTACCA CAGGGAGCTC TACTCTCATC TGGAGCTTTA TGAGATCGGC
    CCCAGTCTCT ACGCCGCTCC ATGGTTCCTC ACCGCCTTTG CCTCACACTT CCCGCTCGGA
    TTTGTGGCAA GAGTATTCGA CATGCTGTTT CTTCAGGGGT CAGAGGTCAT CTTCAAAGTG
    GCCTTGAGTC TTCTCGGGAG CCACAAACCT CTGATTCTCC AACACGACAA TCTTGAGGCA
    ATCGTGGAGT TCATCAAAAC AACCTTACCC AACCTCGGCC TCGTGCAAAT GGAGAAAACT
    ATCAATCAGG TGTCAGAGAT GGATATCAGT AAGCAGCTGC AGGCCTATGA AGTGGAATAT
    CATGTTCTTC AGGATGAGCT TTTGGATGGT CCCTCCACAC TTAGCCATTC CCAGCGAGCG
    GCCCAGTTGG AGAAGACCAA TGGAAGTCTC CGACAACAAA ACTTAGACCT CCTAGAGGAG
    CTACAGGTTG CCCACGCTAA AATCAGATGT TTAGAGACTC GTGTGGACGG CCTTGTGAAC
    CGAGAGGCTG AGCTGCAATC ACAAGTAAAG ACTCTCCAGC AGGAACAAAC AGATCTCCAG
    CAGACCGTAT GCAAACTCAG AGACCTTCTC AGCAGTCTGG GCATAAGCAA CCCCTGCCCA
    GACCTCCAAA TCCTCACAAA CAACAACCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa05632
    Clone ID Related Accession (Same CDS sequence) XM_005168371.3 , XM_005168371.4 , XM_021476253.1
    Accession Version XM_021476253.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3393bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTGAAG CGATGAAAGC GCGCTTTCAC ATCTTCCATA AACTGCGGAG GGATGTGAAA 
    AGCGAGCAGG AGGCAGTTCC AGAGATCATC AGCACTCTCA GACAGGCTGG CAAAAGCTCT
    GCCCGGCAAG AAGACACTCC ATCACTGCCT AGCAACGGTC AATCAGATGC CTCCAGCTCC
    ACGGCAACAG CAGCTGATGC AGCCACAGCC TTCGCTAAAA AGTTTGAGGT GTTGTTCTGC
    GGGCGAGTGG TGGTGGCCCA TAAGAAAGCA CCTCCTGCAC TAATAGATGA GTGTATAGAG
    AAGTTTGGAC GTGTCAGTGT TACAGGCTCA CTTGCAGCGG GCATCAGGCG GGCGTTTTCA
    CTCAATCAGG TGGTGAATGG AACAAATGAG GGTGATGGAG GAGGAAAGCG ACCTTTGTTT
    TTTAAAAGAG ATCAAAGTTT TCCATTTCTG CAGGCATTGG ATGAGAACGG CCTCTCACCG
    GAGCTCCAGC GACGGGCAGC AGACGGAGCC ACTGGCGTTC AACCTACCAG TCTACAGGAG
    AACAGAACCA TGCTGTTTAC AATTGGGCGA TCTCAGATCT TTCTCGTGAG TCCTGACACT
    AAGAAAGTGG CCATAGAGAA GAGCTTCAGA GAGATTTCCT TCTGTTCTCA GGGAATTCGT
    CACGTGGACC ACTTCGGCTT CATATGCAGG GAGTCGGTTG AATGCGGAGG CTGCCAGTTT
    GTTTGCTATG TTTTCCAGTG TGCAAACGAG GCTCTGGTGG ATGAGATCAT GTTGACTTTG
    AAGCAGGCGT TCTCTGTGGC CGCTCTCCAG CAGAACAGCA GGACCCAGAG TCAGCAGTGT
    GACGGCTGTC CCCTGCAGCA GCTGCACAAA CTCTGTGACA GAATAGAGGG TCTTCATCCA
    TCCAAAGCTA AGCTGGAACT GCAGAAGCAT TTGGCAGATC TGGATAATCA GGAGCAGGCA
    GCTGTGTTTG AGAGCACCAT GAAAGCCAGG CCTAAATCGG ATCAGGAGGA AAATGAGTTG
    ATTTTCTCGT GTCTAAGACG GATGTACGAG GACAGACAAA AGACACACAC ACACAACGGG
    CAAACAAAAA AGGATGTTGT TGTGAATGCT GAAAGCAGCG TGAGTTCTAG CCGTGTGCGT
    CTGGAGGAGT TCAAGTCTAG AGCTAAAAGA TCCCTCACTG AGTCACTGGA GGGGATCTGG
    AAGGGTGGCA ATAAGTCAAA ATCCCAGAGG GAAAACAGTG AAGGAGAGAG CAACTCCTCC
    AGTTCTACCA CACTGAGCAG CACACATCAG GAAACATCAT TGCAGGAGCC ACTCTGCTCT
    CTGCTTCTCT CCAGTCCGCT GCGCTCCCAC AATTCCACAG GGAATCTGAA GCATCTTGAA
    GGCTCTGACA GGGGTCAGGA GGATCCTGAG AGTCCGCCTC AGGGTTTCAG GAGACGCGCA
    AGCACTATCA GTCACTCTCC CATGCTGTCG TCCTCTGAGC CGAGCCTCCC TCCTCTACAG
    CCCACTGCTG CCAACAAACC CAAACTGGTC CGACACTACT CCGTCAGCAC AGACTCCCCA
    CACCAGAGCA ATCCTTCCTC CTCTCCGGCT TCTTCCTCTC CTGTGTGTCA GCGGCCGCTG
    AGGCCTCGTT CTTTTGCTCT TGATCATTCC ACACGGCCTC TCAAGCGCAG TCCATCAGGG
    CTGAGTGGCT TTAGAGCGAG ATTACACTCG TCCTCCTCTG TTCCAAATTT CCTCAAATTT
    CTGGCCCCTG TAGTGGAAAG TGATGATACC TGTGACTCTC AGAGGAAGAG TGATGTTGCG
    GCCCTTTCCA GTCCTGGATG TGTTATTGGT GATGACAGTC CATTGCGCAA CCGGAGACAC
    TCATGGAGAC AACAGATCTT CTTAAGAGTC GCTACACCAC AGAAGGGCTC GGACGCCAAA
    GAATCCACGT TAGAGGTTGG CAGCAGAATG GCACTGGGAG GTGGAGTTGG TGGCGGAGAC
    AAAACACTTG GGGCGGTGCC AGAGGAGAGG AGAAAGAGGA GCGGGGCGGA GCTTAGGAAG
    TTATGGAGGA AAGCCATTCT TCAACAGATA CTCCTACTGC GGATGGAGAA AGAGAATCAG
    AAACTACAAG CGTCAGAGAG TGATCTGCAG TGCAAGCGAC AGAAGCTGGA TTATGAAGAG
    ATCACTCCAT GCCTGAAAGA TGTCACTCTG GTCTGGGAGA GGATGCTCGG CACGGCTGGA
    AGAGCAAAGG TCAGATTTGA CATGGATGAG ATCCACGGTG CTGTGGGACA AGGCGTTCCT
    CGTCAGCATC GAGGAGAGAT CTGGAAGTTT CTCTCTGAGC AGTATTTGCT GAGGCAGGAG
    GTGCCCAGTG CCAAACCGCC AAACAATGAC ACTCCTTACA AAGAGCTACT GAAGCAGCTC
    ACATCCCAGC AGCATGCCAT CCTCATTGAC TTGGGTCGTA CATTTCCCAC TCACCCGTAT
    TTCAGCGCTC AGCTTGGTGC AGGCCAGCTG TCCCTCTATA ACCTACTGAA GGCATATTCT
    CTGCTGGACC CGGAGGTCGG TTACTGCCAG GGCCTGAGCT TTGTGGCTGG TGTTTTGCTG
    CTCCACATGA GCGAGGAAGA CGCGTTTCAC ATGCTCAAAT TCCTCATGTA CGACATGGGG
    CTCAGGAAAC AGTACAGGCC TGATATGATC ATACTGCAGA TTCAGATGTA TCAGCTGTCT
    CGTCTGTTGC ATGACTACCA CAGGGAGCTC TACTCTCATC TGGAGCTTTA TGAGATCGGC
    CCCAGTCTCT ACGCCGCTCC ATGGTTCCTC ACCGCCTTTG CCTCACACTT CCCGCTCGGA
    TTTGTGGCAA GAGTATTCGA CATGCTGTTT CTTCAGGGGT CAGAGGTCAT CTTCAAAGTG
    GCCTTGAGTC TTCTCGGGAG CCACAAACCT CTGATTCTCC AACACGACAA TCTTGAGGCA
    ATCGTGGAGT TCATCAAAAC AACCTTACCC AACCTCGGCC TCGTGCAAAT GGAGAAAACT
    ATCAATCAGG TGTCAGAGAT GGATATCAGT AAGCAGCTGC AGGCCTATGA AGTGGAATAT
    CATGTTCTTC AGGATGAGCT TTTGGATGGT CCCTCCACAC TTAGCCATTC CCAGCGAGCG
    GCCCAGTTGG AGAAGACCAA TGGAAGTCTC CGACAACAAA ACTTAGACCT CCTAGAGGAG
    CTACAGGTTG CCCACGCTAA AATCAGATGT TTAGAGACTC GTGTGGACGG CCTTGTGAAC
    CGAGAGGCTG AGCTGCAATC ACAAGTAAAG ACTCTCCAGC AGGAACAAAC AGATCTCCAG
    CAGACCGTAT GCAAACTCAG AGACCTTCTC AGCAGTCTGG GCATAAGCAA CCCCTGCCCA
    GACCTCCAAA TCCTCACAAA CAACAACCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331928.1
    CDS161..3553
    Translation

    Target ORF information:

    RefSeq Version XM_021476253.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476253.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    ATGGCTGAAG CGATGAAAGC GCGCTTTCAC ATCTTCCATA AACTGCGGAG GGATGTGAAA 
    AGCGAGCAGG AGGCAGTTCC AGAGATCATC AGCACTCTCA GACAGGCTGG CAAAAGCTCT
    GCCCGGCAAG AAGACACTCC ATCACTGCCT AGCAACGGTC AATCAGATGC CTCCAGCTCC
    ACGGCAACAG CAGCTGATGC AGCCACAGCC TTCGCTAAAA AGTTTGAGGT GTTGTTCTGC
    GGGCGAGTGG TGGTGGCCCA TAAGAAAGCA CCTCCTGCAC TAATAGATGA GTGTATAGAG
    AAGTTTGGAC GTGTCAGTGT TACAGGCTCA CTTGCAGCGG GCATCAGGCG GGCGTTTTCA
    CTCAATCAGG TGGTGAATGG AACAAATGAG GGTGATGGAG GAGGAAAGCG ACCTTTGTTT
    TTTAAAAGAG ATCAAAGTTT TCCATTTCTG CAGGCATTGG ATGAGAACGG CCTCTCACCG
    GAGCTCCAGC GACGGGCAGC AGACGGAGCC ACTGGCGTTC AACCTACCAG TCTACAGGAG
    AACAGAACCA TGCTGTTTAC AATTGGGCGA TCTCAGATCT TTCTCGTGAG TCCTGACACT
    AAGAAAGTGG CCATAGAGAA GAGCTTCAGA GAGATTTCCT TCTGTTCTCA GGGAATTCGT
    CACGTGGACC ACTTCGGCTT CATATGCAGG GAGTCGGTTG AATGCGGAGG CTGCCAGTTT
    GTTTGCTATG TTTTCCAGTG TGCAAACGAG GCTCTGGTGG ATGAGATCAT GTTGACTTTG
    AAGCAGGCGT TCTCTGTGGC CGCTCTCCAG CAGAACAGCA GGACCCAGAG TCAGCAGTGT
    GACGGCTGTC CCCTGCAGCA GCTGCACAAA CTCTGTGACA GAATAGAGGG TCTTCATCCA
    TCCAAAGCTA AGCTGGAACT GCAGAAGCAT TTGGCAGATC TGGATAATCA GGAGCAGGCA
    GCTGTGTTTG AGAGCACCAT GAAAGCCAGG CCTAAATCGG ATCAGGAGGA AAATGAGTTG
    ATTTTCTCGT GTCTAAGACG GATGTACGAG GACAGACAAA AGACACACAC ACACAACGGG
    CAAACAAAAA AGGATGTTGT TGTGAATGCT GAAAGCAGCG TGAGTTCTAG CCGTGTGCGT
    CTGGAGGAGT TCAAGTCTAG AGCTAAAAGA TCCCTCACTG AGTCACTGGA GGGGATCTGG
    AAGGGTGGCA ATAAGTCAAA ATCCCAGAGG GAAAACAGTG AAGGAGAGAG CAACTCCTCC
    AGTTCTACCA CACTGAGCAG CACACATCAG GAAACATCAT TGCAGGAGCC ACTCTGCTCT
    CTGCTTCTCT CCAGTCCGCT GCGCTCCCAC AATTCCACAG GGAATCTGAA GCATCTTGAA
    GGCTCTGACA GGGGTCAGGA GGATCCTGAG AGTCCGCCTC AGGGTTTCAG GAGACGCGCA
    AGCACTATCA GTCACTCTCC CATGCTGTCG TCCTCTGAGC CGAGCCTCCC TCCTCTACAG
    CCCACTGCTG CCAACAAACC CAAACTGGTC CGACACTACT CCGTCAGCAC AGACTCCCCA
    CACCAGAGCA ATCCTTCCTC CTCTCCGGCT TCTTCCTCTC CTGTGTGTCA GCGGCCGCTG
    AGGCCTCGTT CTTTTGCTCT TGATCATTCC ACACGGCCTC TCAAGCGCAG TCCATCAGGG
    CTGAGTGGCT TTAGAGCGAG ATTACACTCG TCCTCCTCTG TTCCAAATTT CCTCAAATTT
    CTGGCCCCTG TAGTGGAAAG TGATGATACC TGTGACTCTC AGAGGAAGAG TGATGTTGCG
    GCCCTTTCCA GTCCTGGATG TGTTATTGGT GATGACAGTC CATTGCGCAA CCGGAGACAC
    TCATGGAGAC AACAGATCTT CTTAAGAGTC GCTACACCAC AGAAGGGCTC GGACGCCAAA
    GAATCCACGT TAGAGGTTGG CAGCAGAATG GCACTGGGAG GTGGAGTTGG TGGCGGAGAC
    AAAACACTTG GGGCGGTGCC AGAGGAGAGG AGAAAGAGGA GCGGGGCGGA GCTTAGGAAG
    TTATGGAGGA AAGCCATTCT TCAACAGATA CTCCTACTGC GGATGGAGAA AGAGAATCAG
    AAACTACAAG CGTCAGAGAG TGATCTGCAG TGCAAGCGAC AGAAGCTGGA TTATGAAGAG
    ATCACTCCAT GCCTGAAAGA TGTCACTCTG GTCTGGGAGA GGATGCTCGG CACGGCTGGA
    AGAGCAAAGG TCAGATTTGA CATGGATGAG ATCCACGGTG CTGTGGGACA AGGCGTTCCT
    CGTCAGCATC GAGGAGAGAT CTGGAAGTTT CTCTCTGAGC AGTATTTGCT GAGGCAGGAG
    GTGCCCAGTG CCAAACCGCC AAACAATGAC ACTCCTTACA AAGAGCTACT GAAGCAGCTC
    ACATCCCAGC AGCATGCCAT CCTCATTGAC TTGGGTCGTA CATTTCCCAC TCACCCGTAT
    TTCAGCGCTC AGCTTGGTGC AGGCCAGCTG TCCCTCTATA ACCTACTGAA GGCATATTCT
    CTGCTGGACC CGGAGGTCGG TTACTGCCAG GGCCTGAGCT TTGTGGCTGG TGTTTTGCTG
    CTCCACATGA GCGAGGAAGA CGCGTTTCAC ATGCTCAAAT TCCTCATGTA CGACATGGGG
    CTCAGGAAAC AGTACAGGCC TGATATGATC ATACTGCAGA TTCAGATGTA TCAGCTGTCT
    CGTCTGTTGC ATGACTACCA CAGGGAGCTC TACTCTCATC TGGAGCTTTA TGAGATCGGC
    CCCAGTCTCT ACGCCGCTCC ATGGTTCCTC ACCGCCTTTG CCTCACACTT CCCGCTCGGA
    TTTGTGGCAA GAGTATTCGA CATGCTGTTT CTTCAGGGGT CAGAGGTCAT CTTCAAAGTG
    GCCTTGAGTC TTCTCGGGAG CCACAAACCT CTGATTCTCC AACACGACAA TCTTGAGGCA
    ATCGTGGAGT TCATCAAAAC AACCTTACCC AACCTCGGCC TCGTGCAAAT GGAGAAAACT
    ATCAATCAGG TGTCAGAGAT GGATATCAGT AAGCAGCTGC AGGCCTATGA AGTGGAATAT
    CATGTTCTTC AGGATGAGCT TTTGGATGGT CCCTCCACAC TTAGCCATTC CCAGCGAGCG
    GCCCAGTTGG AGAAGACCAA TGGAAGTCTC CGACAACAAA ACTTAGACCT CCTAGAGGAG
    CTACAGGTTG CCCACGCTAA AATCAGATGT TTAGAGACTC GTGTGGACGG CCTTGTGAAC
    CGAGAGGCTG AGCTGCAATC ACAAGTAAAG ACTCTCCAGC AGGAACAAAC AGATCTCCAG
    CAGACCGTAT GCAAACTCAG AGACCTTCTC AGCAGTCTGG GCATAAGCAA CCCCTGCCCA
    GACCTCCAAA TCCTCACAAA CAACAACCAA TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43457
    Clone ID Related Accession (Same CDS sequence) XM_021476256.1 , XM_009307533.3 , XM_009307533.2
    Accession Version XM_021476256.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTTCTC TGCGTAGATG GCTGAATCAG GTTCCAGAGA TCATCAGCAC TCTCAGACAG 
    GCTGGCAAAA GCTCTGCCCG GCAAGAAGAC ACTCCATCAC TGCCTAGCAA CGGTCAATCA
    GATGCCTCCA GCTCCACGGC AACAGCAGCT GATGCAGCCA CAGCCTTCGC TAAAAAGTTT
    GAGGTGTTGT TCTGCGGGCG AGTGGTGGTG GCCCATAAGA AAGCACCTCC TGCACTAATA
    GATGAGTGTA TAGAGAAGTT TGGACGTGTC AGTGTTACAG GCTCACTTGC AGCGGGCATC
    AGGCGGGCGT TTTCACTCAA TCAGGTGGTG AATGGAACAA ATGAGGGTGA TGGAGGAGGA
    AAGCGACCTT TGTTTTTTAA AAGAGATCAA AGTTTTCCAT TTCTGCAGGC ATTGGATGAG
    AACGGCCTCT CACCGGAGCT CCAGCGACGG GCAGCAGACG GAGCCACTGG CGTTCAACCT
    ACCAGTCTAC AGGAGAACAG AACCATGCTG TTTACAATTG GGCGATCTCA GATCTTTCTC
    GTGAGTCCTG ACACTAAGAA AGTGGCCATA GAGAAGAGCT TCAGAGAGAT TTCCTTCTGT
    TCTCAGGGAA TTCGTCACGT GGACCACTTC GGCTTCATAT GCAGGGAGTC GGTTGAATGC
    GGAGGCTGCC AGTTTGTTTG CTATGTTTTC CAGTGTGCAA ACGAGGCTCT GGTGGATGAG
    ATCATGTTGA CTTTGAAGCA GGCGTTCTCT GTGGCCGCTC TCCAGCAGAA CAGCAGGACC
    CAGAGTCAGC AGTGTGACGG CTGTCCCCTG CAGCAGCTGC ACAAACTCTG TGACAGAATA
    GAGGGTCTTC ATCCATCCAA AGCTAAGCTG GAACTGCAGA AGCATTTGGC AGATCTGGAT
    AATCAGGAGC AGGCAGCTGT GTTTGAGAGC ACCATGAAAG CCAGGCCTAA ATCGGATCAG
    GAGGAAAATG AGTTGATTTT CTCGTGTCTA AGACGGATGT ACGAGGACAG ACAAAAGACA
    CACACACACA ACGGGCAAAC AAAAAAGGAT GTTGTTGTGA ATGCTGAAAG CAGCGTGAGT
    TCTAGCCGTG TGCGTCTGGA GGAGTTCAAG TCTAGAGCTA AAAGATCCCT CACTGAGTCA
    CTGGAGGGGA TCTGGAAGGG TGGCAATAAG TCAAAATCCC AGAGGGAAAA CAGTGAAGGA
    GAGAGCAACT CCTCCAGTTC TACCACACTG AGCAGCACAC ATCAGGAAAC ATCATTGCAG
    GAGCCACTCT GCTCTCTGCT TCTCTCCAGT CCGCTGCGCT CCCACAATTC CACAGGGAAT
    CTGAAGCATC TTGAAGGCTC TGACAGGGGT CAGGAGGATC CTGAGAGTCC GCCTCAGGGT
    TTCAGGAGAC GCGCAAGCAC TATCAGTCAC TCTCCCATGC TGTCGTCCTC TGAGCCGAGC
    CTCCCTCCTC TACAGCCCAC TGCTGCCAAC AAACCCAAAC TGGTCCGACA CTACTCCGTC
    AGCACAGACT CCCCACACCA GAGCAATCCT TCCTCCTCTC CGGCTTCTTC CTCTCCTGTG
    TGTCAGCGGC CGCTGAGGCC TCGTTCTTTT GCTCTTGATC ATTCCACACG GCCTCTCAAG
    CGCAGTCCAT CAGGGCTGAG TGGCTTTAGA GCGAGATTAC ACTCGTCCTC CTCTGTTCCA
    AATTTCCTCA AATTTCTGGC CCCTGTAGTG GAAAGTGATG ATACCTGTGA CTCTCAGAGG
    AAGAGTGATG TTGCGGCCCT TTCCAGTCCT GGATGTGTTA TTGGTGATGA CAGTCCATTG
    CGCAACCGGA GACACTCATG GAGACAACAG ATCTTCTTAA GAGTCGCTAC ACCACAGAAG
    GGCTCGGACG CCAAAGAATC CACGTTAGAG GTTGGCAGCA GAATGGCACT GGGAGGTGGA
    GTTGGTGGCG GAGACAAAAC ACTTGGGGCG GTGCCAGAGG AGAGGAGAAA GAGGAGCGGG
    GCGGAGCTTA GGAAGTTATG GAGGAAAGCC ATTCTTCAAC AGATACTCCT ACTGCGGATG
    GAGAAAGAGA ATCAGAAACT ACAAGCGTCA GAGAGTGATC TGCAGTGCAA GCGACAGAAG
    CTGGATTATG AAGAGATCAC TCCATGCCTG AAAGATGTCA CTCTGGTCTG GGAGAGGATG
    CTCGGCACGG CTGGAAGAGC AAAGGTCAGA TTTGACATGG ATGAGATCCA CGGTGCTGTG
    GGACAAGGCG TTCCTCGTCA GCATCGAGGA GAGATCTGGA AGTTTCTCTC TGAGCAGTAT
    TTGCTGAGGC AGGAGGTGCC CAGTGCCAAA CCGCCAAACA ATGACACTCC TTACAAAGAG
    CTACTGAAGC AGCTCACATC CCAGCAGCAT GCCATCCTCA TTGACTTGGG TCGTACATTT
    CCCACTCACC CGTATTTCAG CGCTCAGCTT GGTGCAGGCC AGCTGTCCCT CTATAACCTA
    CTGAAGGCAT ATTCTCTGCT GGACCCGGAG GTCGGTTACT GCCAGGGCCT GAGCTTTGTG
    GCTGGTGTTT TGCTGCTCCA CATGAGCGAG GAAGACGCGT TTCACATGCT CAAATTCCTC
    ATGTACGACA TGGGGCTCAG GAAACAGTAC AGGCCTGATA TGATCATACT GCAGATTCAG
    ATGTATCAGC TGTCTCGTCT GTTGCATGAC TACCACAGGG AGCTCTACTC TCATCTGGAG
    CTTTATGAGA TCGGCCCCAG TCTCTACGCC GCTCCATGGT TCCTCACCGC CTTTGCCTCA
    CACTTCCCGC TCGGATTTGT GGCAAGAGTA TTCGACATGC TGTTTCTTCA GGGGTCAGAG
    GTCATCTTCA AAGTGGCCTT GAGTCTTCTC GGGAGCCACA AACCTCTGAT TCTCCAACAC
    GACAATCTTG AGGCAATCGT GGAGTTCATC AAAACAACCT TACCCAACCT CGGCCTCGTG
    CAAATGGAGA AAACTATCAA TCAGGTGTCA GAGATGGATA TCAGTAAGCA GCTGCAGGCC
    TATGAAGTGG AATATCATGT TCTTCAGGAT GAGCTTTTGG ATGGTCCCTC CACACTTAGC
    CATTCCCAGC GAGCGGCCCA GTTGGAGAAG ACCAATGGAA GTCTCCGACA ACAAAACTTA
    GACCTCCTAG AGGAGCTACA GGTTGCCCAC GCTAAAATCA GATGTTTAGA GACTCGTGTG
    GACGGCCTTG TGAACCGAGA GGCTGAGCTG CAATCACAAG TAAAGACTCT CCAGCAGGAA
    CAAACAGATC TCCAGCAGAC CGTATGCAAA CTCAGAGACC TTCTCAGCAG TCTGGGCATA
    AGCAACCCCT GCCCAGACCT CCAAATCCTC ACAAACAACA ACCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021331931.1
    CDS163..3510
    Translation

    Target ORF information:

    RefSeq Version XM_021476256.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021476256.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTTCTC TGCGTAGATG GCTGAATCAG GTTCCAGAGA TCATCAGCAC TCTCAGACAG 
    GCTGGCAAAA GCTCTGCCCG GCAAGAAGAC ACTCCATCAC TGCCTAGCAA CGGTCAATCA
    GATGCCTCCA GCTCCACGGC AACAGCAGCT GATGCAGCCA CAGCCTTCGC TAAAAAGTTT
    GAGGTGTTGT TCTGCGGGCG AGTGGTGGTG GCCCATAAGA AAGCACCTCC TGCACTAATA
    GATGAGTGTA TAGAGAAGTT TGGACGTGTC AGTGTTACAG GCTCACTTGC AGCGGGCATC
    AGGCGGGCGT TTTCACTCAA TCAGGTGGTG AATGGAACAA ATGAGGGTGA TGGAGGAGGA
    AAGCGACCTT TGTTTTTTAA AAGAGATCAA AGTTTTCCAT TTCTGCAGGC ATTGGATGAG
    AACGGCCTCT CACCGGAGCT CCAGCGACGG GCAGCAGACG GAGCCACTGG CGTTCAACCT
    ACCAGTCTAC AGGAGAACAG AACCATGCTG TTTACAATTG GGCGATCTCA GATCTTTCTC
    GTGAGTCCTG ACACTAAGAA AGTGGCCATA GAGAAGAGCT TCAGAGAGAT TTCCTTCTGT
    TCTCAGGGAA TTCGTCACGT GGACCACTTC GGCTTCATAT GCAGGGAGTC GGTTGAATGC
    GGAGGCTGCC AGTTTGTTTG CTATGTTTTC CAGTGTGCAA ACGAGGCTCT GGTGGATGAG
    ATCATGTTGA CTTTGAAGCA GGCGTTCTCT GTGGCCGCTC TCCAGCAGAA CAGCAGGACC
    CAGAGTCAGC AGTGTGACGG CTGTCCCCTG CAGCAGCTGC ACAAACTCTG TGACAGAATA
    GAGGGTCTTC ATCCATCCAA AGCTAAGCTG GAACTGCAGA AGCATTTGGC AGATCTGGAT
    AATCAGGAGC AGGCAGCTGT GTTTGAGAGC ACCATGAAAG CCAGGCCTAA ATCGGATCAG
    GAGGAAAATG AGTTGATTTT CTCGTGTCTA AGACGGATGT ACGAGGACAG ACAAAAGACA
    CACACACACA ACGGGCAAAC AAAAAAGGAT GTTGTTGTGA ATGCTGAAAG CAGCGTGAGT
    TCTAGCCGTG TGCGTCTGGA GGAGTTCAAG TCTAGAGCTA AAAGATCCCT CACTGAGTCA
    CTGGAGGGGA TCTGGAAGGG TGGCAATAAG TCAAAATCCC AGAGGGAAAA CAGTGAAGGA
    GAGAGCAACT CCTCCAGTTC TACCACACTG AGCAGCACAC ATCAGGAAAC ATCATTGCAG
    GAGCCACTCT GCTCTCTGCT TCTCTCCAGT CCGCTGCGCT CCCACAATTC CACAGGGAAT
    CTGAAGCATC TTGAAGGCTC TGACAGGGGT CAGGAGGATC CTGAGAGTCC GCCTCAGGGT
    TTCAGGAGAC GCGCAAGCAC TATCAGTCAC TCTCCCATGC TGTCGTCCTC TGAGCCGAGC
    CTCCCTCCTC TACAGCCCAC TGCTGCCAAC AAACCCAAAC TGGTCCGACA CTACTCCGTC
    AGCACAGACT CCCCACACCA GAGCAATCCT TCCTCCTCTC CGGCTTCTTC CTCTCCTGTG
    TGTCAGCGGC CGCTGAGGCC TCGTTCTTTT GCTCTTGATC ATTCCACACG GCCTCTCAAG
    CGCAGTCCAT CAGGGCTGAG TGGCTTTAGA GCGAGATTAC ACTCGTCCTC CTCTGTTCCA
    AATTTCCTCA AATTTCTGGC CCCTGTAGTG GAAAGTGATG ATACCTGTGA CTCTCAGAGG
    AAGAGTGATG TTGCGGCCCT TTCCAGTCCT GGATGTGTTA TTGGTGATGA CAGTCCATTG
    CGCAACCGGA GACACTCATG GAGACAACAG ATCTTCTTAA GAGTCGCTAC ACCACAGAAG
    GGCTCGGACG CCAAAGAATC CACGTTAGAG GTTGGCAGCA GAATGGCACT GGGAGGTGGA
    GTTGGTGGCG GAGACAAAAC ACTTGGGGCG GTGCCAGAGG AGAGGAGAAA GAGGAGCGGG
    GCGGAGCTTA GGAAGTTATG GAGGAAAGCC ATTCTTCAAC AGATACTCCT ACTGCGGATG
    GAGAAAGAGA ATCAGAAACT ACAAGCGTCA GAGAGTGATC TGCAGTGCAA GCGACAGAAG
    CTGGATTATG AAGAGATCAC TCCATGCCTG AAAGATGTCA CTCTGGTCTG GGAGAGGATG
    CTCGGCACGG CTGGAAGAGC AAAGGTCAGA TTTGACATGG ATGAGATCCA CGGTGCTGTG
    GGACAAGGCG TTCCTCGTCA GCATCGAGGA GAGATCTGGA AGTTTCTCTC TGAGCAGTAT
    TTGCTGAGGC AGGAGGTGCC CAGTGCCAAA CCGCCAAACA ATGACACTCC TTACAAAGAG
    CTACTGAAGC AGCTCACATC CCAGCAGCAT GCCATCCTCA TTGACTTGGG TCGTACATTT
    CCCACTCACC CGTATTTCAG CGCTCAGCTT GGTGCAGGCC AGCTGTCCCT CTATAACCTA
    CTGAAGGCAT ATTCTCTGCT GGACCCGGAG GTCGGTTACT GCCAGGGCCT GAGCTTTGTG
    GCTGGTGTTT TGCTGCTCCA CATGAGCGAG GAAGACGCGT TTCACATGCT CAAATTCCTC
    ATGTACGACA TGGGGCTCAG GAAACAGTAC AGGCCTGATA TGATCATACT GCAGATTCAG
    ATGTATCAGC TGTCTCGTCT GTTGCATGAC TACCACAGGG AGCTCTACTC TCATCTGGAG
    CTTTATGAGA TCGGCCCCAG TCTCTACGCC GCTCCATGGT TCCTCACCGC CTTTGCCTCA
    CACTTCCCGC TCGGATTTGT GGCAAGAGTA TTCGACATGC TGTTTCTTCA GGGGTCAGAG
    GTCATCTTCA AAGTGGCCTT GAGTCTTCTC GGGAGCCACA AACCTCTGAT TCTCCAACAC
    GACAATCTTG AGGCAATCGT GGAGTTCATC AAAACAACCT TACCCAACCT CGGCCTCGTG
    CAAATGGAGA AAACTATCAA TCAGGTGTCA GAGATGGATA TCAGTAAGCA GCTGCAGGCC
    TATGAAGTGG AATATCATGT TCTTCAGGAT GAGCTTTTGG ATGGTCCCTC CACACTTAGC
    CATTCCCAGC GAGCGGCCCA GTTGGAGAAG ACCAATGGAA GTCTCCGACA ACAAAACTTA
    GACCTCCTAG AGGAGCTACA GGTTGCCCAC GCTAAAATCA GATGTTTAGA GACTCGTGTG
    GACGGCCTTG TGAACCGAGA GGCTGAGCTG CAATCACAAG TAAAGACTCT CCAGCAGGAA
    CAAACAGATC TCCAGCAGAC CGTATGCAAA CTCAGAGACC TTCTCAGCAG TCTGGGCATA
    AGCAACCCCT GCCCAGACCT CCAAATCCTC ACAAACAACA ACCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43457
    Clone ID Related Accession (Same CDS sequence) XM_021476256.1 , XM_009307533.3 , XM_009307533.2
    Accession Version XM_009307533.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product TBC1 domain family member 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394473.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009307533.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTTCTC TGCGTAGATG GCTGAATCAG GTTCCAGAGA TCATCAGCAC TCTCAGACAG 
    GCTGGCAAAA GCTCTGCCCG GCAAGAAGAC ACTCCATCAC TGCCTAGCAA CGGTCAATCA
    GATGCCTCCA GCTCCACGGC AACAGCAGCT GATGCAGCCA CAGCCTTCGC TAAAAAGTTT
    GAGGTGTTGT TCTGCGGGCG AGTGGTGGTG GCCCATAAGA AAGCACCTCC TGCACTAATA
    GATGAGTGTA TAGAGAAGTT TGGACGTGTC AGTGTTACAG GCTCACTTGC AGCGGGCATC
    AGGCGGGCGT TTTCACTCAA TCAGGTGGTG AATGGAACAA ATGAGGGTGA TGGAGGAGGA
    AAGCGACCTT TGTTTTTTAA AAGAGATCAA AGTTTTCCAT TTCTGCAGGC ATTGGATGAG
    AACGGCCTCT CACCGGAGCT CCAGCGACGG GCAGCAGACG GAGCCACTGG CGTTCAACCT
    ACCAGTCTAC AGGAGAACAG AACCATGCTG TTTACAATTG GGCGATCTCA GATCTTTCTC
    GTGAGTCCTG ACACTAAGAA AGTGGCCATA GAGAAGAGCT TCAGAGAGAT TTCCTTCTGT
    TCTCAGGGAA TTCGTCACGT GGACCACTTC GGCTTCATAT GCAGGGAGTC GGTTGAATGC
    GGAGGCTGCC AGTTTGTTTG CTATGTTTTC CAGTGTGCAA ACGAGGCTCT GGTGGATGAG
    ATCATGTTGA CTTTGAAGCA GGCGTTCTCT GTGGCCGCTC TCCAGCAGAA CAGCAGGACC
    CAGAGTCAGC AGTGTGACGG CTGTCCCCTG CAGCAGCTGC ACAAACTCTG TGACAGAATA
    GAGGGTCTTC ATCCATCCAA AGCTAAGCTG GAACTGCAGA AGCATTTGGC AGATCTGGAT
    AATCAGGAGC AGGCAGCTGT GTTTGAGAGC ACCATGAAAG CCAGGCCTAA ATCGGATCAG
    GAGGAAAATG AGTTGATTTT CTCGTGTCTA AGACGGATGT ACGAGGACAG ACAAAAGACA
    CACACACACA ACGGGCAAAC AAAAAAGGAT GTTGTTGTGA ATGCTGAAAG CAGCGTGAGT
    TCTAGCCGTG TGCGTCTGGA GGAGTTCAAG TCTAGAGCTA AAAGATCCCT CACTGAGTCA
    CTGGAGGGGA TCTGGAAGGG TGGCAATAAG TCAAAATCCC AGAGGGAAAA CAGTGAAGGA
    GAGAGCAACT CCTCCAGTTC TACCACACTG AGCAGCACAC ATCAGGAAAC ATCATTGCAG
    GAGCCACTCT GCTCTCTGCT TCTCTCCAGT CCGCTGCGCT CCCACAATTC CACAGGGAAT
    CTGAAGCATC TTGAAGGCTC TGACAGGGGT CAGGAGGATC CTGAGAGTCC GCCTCAGGGT
    TTCAGGAGAC GCGCAAGCAC TATCAGTCAC TCTCCCATGC TGTCGTCCTC TGAGCCGAGC
    CTCCCTCCTC TACAGCCCAC TGCTGCCAAC AAACCCAAAC TGGTCCGACA CTACTCCGTC
    AGCACAGACT CCCCACACCA GAGCAATCCT TCCTCCTCTC CGGCTTCTTC CTCTCCTGTG
    TGTCAGCGGC CGCTGAGGCC TCGTTCTTTT GCTCTTGATC ATTCCACACG GCCTCTCAAG
    CGCAGTCCAT CAGGGCTGAG TGGCTTTAGA GCGAGATTAC ACTCGTCCTC CTCTGTTCCA
    AATTTCCTCA AATTTCTGGC CCCTGTAGTG GAAAGTGATG ATACCTGTGA CTCTCAGAGG
    AAGAGTGATG TTGCGGCCCT TTCCAGTCCT GGATGTGTTA TTGGTGATGA CAGTCCATTG
    CGCAACCGGA GACACTCATG GAGACAACAG ATCTTCTTAA GAGTCGCTAC ACCACAGAAG
    GGCTCGGACG CCAAAGAATC CACGTTAGAG GTTGGCAGCA GAATGGCACT GGGAGGTGGA
    GTTGGTGGCG GAGACAAAAC ACTTGGGGCG GTGCCAGAGG AGAGGAGAAA GAGGAGCGGG
    GCGGAGCTTA GGAAGTTATG GAGGAAAGCC ATTCTTCAAC AGATACTCCT ACTGCGGATG
    GAGAAAGAGA ATCAGAAACT ACAAGCGTCA GAGAGTGATC TGCAGTGCAA GCGACAGAAG
    CTGGATTATG AAGAGATCAC TCCATGCCTG AAAGATGTCA CTCTGGTCTG GGAGAGGATG
    CTCGGCACGG CTGGAAGAGC AAAGGTCAGA TTTGACATGG ATGAGATCCA CGGTGCTGTG
    GGACAAGGCG TTCCTCGTCA GCATCGAGGA GAGATCTGGA AGTTTCTCTC TGAGCAGTAT
    TTGCTGAGGC AGGAGGTGCC CAGTGCCAAA CCGCCAAACA ATGACACTCC TTACAAAGAG
    CTACTGAAGC AGCTCACATC CCAGCAGCAT GCCATCCTCA TTGACTTGGG TCGTACATTT
    CCCACTCACC CGTATTTCAG CGCTCAGCTT GGTGCAGGCC AGCTGTCCCT CTATAACCTA
    CTGAAGGCAT ATTCTCTGCT GGACCCGGAG GTCGGTTACT GCCAGGGCCT GAGCTTTGTG
    GCTGGTGTTT TGCTGCTCCA CATGAGCGAG GAAGACGCGT TTCACATGCT CAAATTCCTC
    ATGTACGACA TGGGGCTCAG GAAACAGTAC AGGCCTGATA TGATCATACT GCAGATTCAG
    ATGTATCAGC TGTCTCGTCT GTTGCATGAC TACCACAGGG AGCTCTACTC TCATCTGGAG
    CTTTATGAGA TCGGCCCCAG TCTCTACGCC GCTCCATGGT TCCTCACCGC CTTTGCCTCA
    CACTTCCCGC TCGGATTTGT GGCAAGAGTA TTCGACATGC TGTTTCTTCA GGGGTCAGAG
    GTCATCTTCA AAGTGGCCTT GAGTCTTCTC GGGAGCCACA AACCTCTGAT TCTCCAACAC
    GACAATCTTG AGGCAATCGT GGAGTTCATC AAAACAACCT TACCCAACCT CGGCCTCGTG
    CAAATGGAGA AAACTATCAA TCAGGTGTCA GAGATGGATA TCAGTAAGCA GCTGCAGGCC
    TATGAAGTGG AATATCATGT TCTTCAGGAT GAGCTTTTGG ATGGTCCCTC CACACTTAGC
    CATTCCCAGC GAGCGGCCCA GTTGGAGAAG ACCAATGGAA GTCTCCGACA ACAAAACTTA
    GACCTCCTAG AGGAGCTACA GGTTGCCCAC GCTAAAATCA GATGTTTAGA GACTCGTGTG
    GACGGCCTTG TGAACCGAGA GGCTGAGCTG CAATCACAAG TAAAGACTCT CCAGCAGGAA
    CAAACAGATC TCCAGCAGAC CGTATGCAAA CTCAGAGACC TTCTCAGCAG TCTGGGCATA
    AGCAACCCCT GCCCAGACCT CCAAATCCTC ACAAACAACA ACCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305808.1
    CDS163..3510
    Translation

    Target ORF information:

    RefSeq Version XM_009307533.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307533.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTTCTC TGCGTAGATG GCTGAATCAG GTTCCAGAGA TCATCAGCAC TCTCAGACAG 
    GCTGGCAAAA GCTCTGCCCG GCAAGAAGAC ACTCCATCAC TGCCTAGCAA CGGTCAATCA
    GATGCCTCCA GCTCCACGGC AACAGCAGCT GATGCAGCCA CAGCCTTCGC TAAAAAGTTT
    GAGGTGTTGT TCTGCGGGCG AGTGGTGGTG GCCCATAAGA AAGCACCTCC TGCACTAATA
    GATGAGTGTA TAGAGAAGTT TGGACGTGTC AGTGTTACAG GCTCACTTGC AGCGGGCATC
    AGGCGGGCGT TTTCACTCAA TCAGGTGGTG AATGGAACAA ATGAGGGTGA TGGAGGAGGA
    AAGCGACCTT TGTTTTTTAA AAGAGATCAA AGTTTTCCAT TTCTGCAGGC ATTGGATGAG
    AACGGCCTCT CACCGGAGCT CCAGCGACGG GCAGCAGACG GAGCCACTGG CGTTCAACCT
    ACCAGTCTAC AGGAGAACAG AACCATGCTG TTTACAATTG GGCGATCTCA GATCTTTCTC
    GTGAGTCCTG ACACTAAGAA AGTGGCCATA GAGAAGAGCT TCAGAGAGAT TTCCTTCTGT
    TCTCAGGGAA TTCGTCACGT GGACCACTTC GGCTTCATAT GCAGGGAGTC GGTTGAATGC
    GGAGGCTGCC AGTTTGTTTG CTATGTTTTC CAGTGTGCAA ACGAGGCTCT GGTGGATGAG
    ATCATGTTGA CTTTGAAGCA GGCGTTCTCT GTGGCCGCTC TCCAGCAGAA CAGCAGGACC
    CAGAGTCAGC AGTGTGACGG CTGTCCCCTG CAGCAGCTGC ACAAACTCTG TGACAGAATA
    GAGGGTCTTC ATCCATCCAA AGCTAAGCTG GAACTGCAGA AGCATTTGGC AGATCTGGAT
    AATCAGGAGC AGGCAGCTGT GTTTGAGAGC ACCATGAAAG CCAGGCCTAA ATCGGATCAG
    GAGGAAAATG AGTTGATTTT CTCGTGTCTA AGACGGATGT ACGAGGACAG ACAAAAGACA
    CACACACACA ACGGGCAAAC AAAAAAGGAT GTTGTTGTGA ATGCTGAAAG CAGCGTGAGT
    TCTAGCCGTG TGCGTCTGGA GGAGTTCAAG TCTAGAGCTA AAAGATCCCT CACTGAGTCA
    CTGGAGGGGA TCTGGAAGGG TGGCAATAAG TCAAAATCCC AGAGGGAAAA CAGTGAAGGA
    GAGAGCAACT CCTCCAGTTC TACCACACTG AGCAGCACAC ATCAGGAAAC ATCATTGCAG
    GAGCCACTCT GCTCTCTGCT TCTCTCCAGT CCGCTGCGCT CCCACAATTC CACAGGGAAT
    CTGAAGCATC TTGAAGGCTC TGACAGGGGT CAGGAGGATC CTGAGAGTCC GCCTCAGGGT
    TTCAGGAGAC GCGCAAGCAC TATCAGTCAC TCTCCCATGC TGTCGTCCTC TGAGCCGAGC
    CTCCCTCCTC TACAGCCCAC TGCTGCCAAC AAACCCAAAC TGGTCCGACA CTACTCCGTC
    AGCACAGACT CCCCACACCA GAGCAATCCT TCCTCCTCTC CGGCTTCTTC CTCTCCTGTG
    TGTCAGCGGC CGCTGAGGCC TCGTTCTTTT GCTCTTGATC ATTCCACACG GCCTCTCAAG
    CGCAGTCCAT CAGGGCTGAG TGGCTTTAGA GCGAGATTAC ACTCGTCCTC CTCTGTTCCA
    AATTTCCTCA AATTTCTGGC CCCTGTAGTG GAAAGTGATG ATACCTGTGA CTCTCAGAGG
    AAGAGTGATG TTGCGGCCCT TTCCAGTCCT GGATGTGTTA TTGGTGATGA CAGTCCATTG
    CGCAACCGGA GACACTCATG GAGACAACAG ATCTTCTTAA GAGTCGCTAC ACCACAGAAG
    GGCTCGGACG CCAAAGAATC CACGTTAGAG GTTGGCAGCA GAATGGCACT GGGAGGTGGA
    GTTGGTGGCG GAGACAAAAC ACTTGGGGCG GTGCCAGAGG AGAGGAGAAA GAGGAGCGGG
    GCGGAGCTTA GGAAGTTATG GAGGAAAGCC ATTCTTCAAC AGATACTCCT ACTGCGGATG
    GAGAAAGAGA ATCAGAAACT ACAAGCGTCA GAGAGTGATC TGCAGTGCAA GCGACAGAAG
    CTGGATTATG AAGAGATCAC TCCATGCCTG AAAGATGTCA CTCTGGTCTG GGAGAGGATG
    CTCGGCACGG CTGGAAGAGC AAAGGTCAGA TTTGACATGG ATGAGATCCA CGGTGCTGTG
    GGACAAGGCG TTCCTCGTCA GCATCGAGGA GAGATCTGGA AGTTTCTCTC TGAGCAGTAT
    TTGCTGAGGC AGGAGGTGCC CAGTGCCAAA CCGCCAAACA ATGACACTCC TTACAAAGAG
    CTACTGAAGC AGCTCACATC CCAGCAGCAT GCCATCCTCA TTGACTTGGG TCGTACATTT
    CCCACTCACC CGTATTTCAG CGCTCAGCTT GGTGCAGGCC AGCTGTCCCT CTATAACCTA
    CTGAAGGCAT ATTCTCTGCT GGACCCGGAG GTCGGTTACT GCCAGGGCCT GAGCTTTGTG
    GCTGGTGTTT TGCTGCTCCA CATGAGCGAG GAAGACGCGT TTCACATGCT CAAATTCCTC
    ATGTACGACA TGGGGCTCAG GAAACAGTAC AGGCCTGATA TGATCATACT GCAGATTCAG
    ATGTATCAGC TGTCTCGTCT GTTGCATGAC TACCACAGGG AGCTCTACTC TCATCTGGAG
    CTTTATGAGA TCGGCCCCAG TCTCTACGCC GCTCCATGGT TCCTCACCGC CTTTGCCTCA
    CACTTCCCGC TCGGATTTGT GGCAAGAGTA TTCGACATGC TGTTTCTTCA GGGGTCAGAG
    GTCATCTTCA AAGTGGCCTT GAGTCTTCTC GGGAGCCACA AACCTCTGAT TCTCCAACAC
    GACAATCTTG AGGCAATCGT GGAGTTCATC AAAACAACCT TACCCAACCT CGGCCTCGTG
    CAAATGGAGA AAACTATCAA TCAGGTGTCA GAGATGGATA TCAGTAAGCA GCTGCAGGCC
    TATGAAGTGG AATATCATGT TCTTCAGGAT GAGCTTTTGG ATGGTCCCTC CACACTTAGC
    CATTCCCAGC GAGCGGCCCA GTTGGAGAAG ACCAATGGAA GTCTCCGACA ACAAAACTTA
    GACCTCCTAG AGGAGCTACA GGTTGCCCAC GCTAAAATCA GATGTTTAGA GACTCGTGTG
    GACGGCCTTG TGAACCGAGA GGCTGAGCTG CAATCACAAG TAAAGACTCT CCAGCAGGAA
    CAAACAGATC TCCAGCAGAC CGTATGCAAA CTCAGAGACC TTCTCAGCAG TCTGGGCATA
    AGCAACCCCT GCCCAGACCT CCAAATCCTC ACAAACAACA ACCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43457
    Clone ID Related Accession (Same CDS sequence) XM_021476256.1 , XM_009307533.3 , XM_009307533.2
    Accession Version XM_009307533.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3348bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product TBC1 domain family member 1 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009307533.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTTCTC TGCGTAGATG GCTGAATCAG GTTCCAGAGA TCATCAGCAC TCTCAGACAG 
    GCTGGCAAAA GCTCTGCCCG GCAAGAAGAC ACTCCATCAC TGCCTAGCAA CGGTCAATCA
    GATGCCTCCA GCTCCACGGC AACAGCAGCT GATGCAGCCA CAGCCTTCGC TAAAAAGTTT
    GAGGTGTTGT TCTGCGGGCG AGTGGTGGTG GCCCATAAGA AAGCACCTCC TGCACTAATA
    GATGAGTGTA TAGAGAAGTT TGGACGTGTC AGTGTTACAG GCTCACTTGC AGCGGGCATC
    AGGCGGGCGT TTTCACTCAA TCAGGTGGTG AATGGAACAA ATGAGGGTGA TGGAGGAGGA
    AAGCGACCTT TGTTTTTTAA AAGAGATCAA AGTTTTCCAT TTCTGCAGGC ATTGGATGAG
    AACGGCCTCT CACCGGAGCT CCAGCGACGG GCAGCAGACG GAGCCACTGG CGTTCAACCT
    ACCAGTCTAC AGGAGAACAG AACCATGCTG TTTACAATTG GGCGATCTCA GATCTTTCTC
    GTGAGTCCTG ACACTAAGAA AGTGGCCATA GAGAAGAGCT TCAGAGAGAT TTCCTTCTGT
    TCTCAGGGAA TTCGTCACGT GGACCACTTC GGCTTCATAT GCAGGGAGTC GGTTGAATGC
    GGAGGCTGCC AGTTTGTTTG CTATGTTTTC CAGTGTGCAA ACGAGGCTCT GGTGGATGAG
    ATCATGTTGA CTTTGAAGCA GGCGTTCTCT GTGGCCGCTC TCCAGCAGAA CAGCAGGACC
    CAGAGTCAGC AGTGTGACGG CTGTCCCCTG CAGCAGCTGC ACAAACTCTG TGACAGAATA
    GAGGGTCTTC ATCCATCCAA AGCTAAGCTG GAACTGCAGA AGCATTTGGC AGATCTGGAT
    AATCAGGAGC AGGCAGCTGT GTTTGAGAGC ACCATGAAAG CCAGGCCTAA ATCGGATCAG
    GAGGAAAATG AGTTGATTTT CTCGTGTCTA AGACGGATGT ACGAGGACAG ACAAAAGACA
    CACACACACA ACGGGCAAAC AAAAAAGGAT GTTGTTGTGA ATGCTGAAAG CAGCGTGAGT
    TCTAGCCGTG TGCGTCTGGA GGAGTTCAAG TCTAGAGCTA AAAGATCCCT CACTGAGTCA
    CTGGAGGGGA TCTGGAAGGG TGGCAATAAG TCAAAATCCC AGAGGGAAAA CAGTGAAGGA
    GAGAGCAACT CCTCCAGTTC TACCACACTG AGCAGCACAC ATCAGGAAAC ATCATTGCAG
    GAGCCACTCT GCTCTCTGCT TCTCTCCAGT CCGCTGCGCT CCCACAATTC CACAGGGAAT
    CTGAAGCATC TTGAAGGCTC TGACAGGGGT CAGGAGGATC CTGAGAGTCC GCCTCAGGGT
    TTCAGGAGAC GCGCAAGCAC TATCAGTCAC TCTCCCATGC TGTCGTCCTC TGAGCCGAGC
    CTCCCTCCTC TACAGCCCAC TGCTGCCAAC AAACCCAAAC TGGTCCGACA CTACTCCGTC
    AGCACAGACT CCCCACACCA GAGCAATCCT TCCTCCTCTC CGGCTTCTTC CTCTCCTGTG
    TGTCAGCGGC CGCTGAGGCC TCGTTCTTTT GCTCTTGATC ATTCCACACG GCCTCTCAAG
    CGCAGTCCAT CAGGGCTGAG TGGCTTTAGA GCGAGATTAC ACTCGTCCTC CTCTGTTCCA
    AATTTCCTCA AATTTCTGGC CCCTGTAGTG GAAAGTGATG ATACCTGTGA CTCTCAGAGG
    AAGAGTGATG TTGCGGCCCT TTCCAGTCCT GGATGTGTTA TTGGTGATGA CAGTCCATTG
    CGCAACCGGA GACACTCATG GAGACAACAG ATCTTCTTAA GAGTCGCTAC ACCACAGAAG
    GGCTCGGACG CCAAAGAATC CACGTTAGAG GTTGGCAGCA GAATGGCACT GGGAGGTGGA
    GTTGGTGGCG GAGACAAAAC ACTTGGGGCG GTGCCAGAGG AGAGGAGAAA GAGGAGCGGG
    GCGGAGCTTA GGAAGTTATG GAGGAAAGCC ATTCTTCAAC AGATACTCCT ACTGCGGATG
    GAGAAAGAGA ATCAGAAACT ACAAGCGTCA GAGAGTGATC TGCAGTGCAA GCGACAGAAG
    CTGGATTATG AAGAGATCAC TCCATGCCTG AAAGATGTCA CTCTGGTCTG GGAGAGGATG
    CTCGGCACGG CTGGAAGAGC AAAGGTCAGA TTTGACATGG ATGAGATCCA CGGTGCTGTG
    GGACAAGGCG TTCCTCGTCA GCATCGAGGA GAGATCTGGA AGTTTCTCTC TGAGCAGTAT
    TTGCTGAGGC AGGAGGTGCC CAGTGCCAAA CCGCCAAACA ATGACACTCC TTACAAAGAG
    CTACTGAAGC AGCTCACATC CCAGCAGCAT GCCATCCTCA TTGACTTGGG TCGTACATTT
    CCCACTCACC CGTATTTCAG CGCTCAGCTT GGTGCAGGCC AGCTGTCCCT CTATAACCTA
    CTGAAGGCAT ATTCTCTGCT GGACCCGGAG GTCGGTTACT GCCAGGGCCT GAGCTTTGTG
    GCTGGTGTTT TGCTGCTCCA CATGAGCGAG GAAGACGCGT TTCACATGCT CAAATTCCTC
    ATGTACGACA TGGGGCTCAG GAAACAGTAC AGGCCTGATA TGATCATACT GCAGATTCAG
    ATGTATCAGC TGTCTCGTCT GTTGCATGAC TACCACAGGG AGCTCTACTC TCATCTGGAG
    CTTTATGAGA TCGGCCCCAG TCTCTACGCC GCTCCATGGT TCCTCACCGC CTTTGCCTCA
    CACTTCCCGC TCGGATTTGT GGCAAGAGTA TTCGACATGC TGTTTCTTCA GGGGTCAGAG
    GTCATCTTCA AAGTGGCCTT GAGTCTTCTC GGGAGCCACA AACCTCTGAT TCTCCAACAC
    GACAATCTTG AGGCAATCGT GGAGTTCATC AAAACAACCT TACCCAACCT CGGCCTCGTG
    CAAATGGAGA AAACTATCAA TCAGGTGTCA GAGATGGATA TCAGTAAGCA GCTGCAGGCC
    TATGAAGTGG AATATCATGT TCTTCAGGAT GAGCTTTTGG ATGGTCCCTC CACACTTAGC
    CATTCCCAGC GAGCGGCCCA GTTGGAGAAG ACCAATGGAA GTCTCCGACA ACAAAACTTA
    GACCTCCTAG AGGAGCTACA GGTTGCCCAC GCTAAAATCA GATGTTTAGA GACTCGTGTG
    GACGGCCTTG TGAACCGAGA GGCTGAGCTG CAATCACAAG TAAAGACTCT CCAGCAGGAA
    CAAACAGATC TCCAGCAGAC CGTATGCAAA CTCAGAGACC TTCTCAGCAG TCTGGGCATA
    AGCAACCCCT GCCCAGACCT CCAAATCCTC ACAAACAACA ACCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009305808.1
    CDS167..3514
    Translation

    Target ORF information:

    RefSeq Version XM_009307533.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio TBC1 (tre-2/USP6, BUB2, cdc16) domain family, member 1 (tbc1d1), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009307533.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGCCTTCTC TGCGTAGATG GCTGAATCAG GTTCCAGAGA TCATCAGCAC TCTCAGACAG 
    GCTGGCAAAA GCTCTGCCCG GCAAGAAGAC ACTCCATCAC TGCCTAGCAA CGGTCAATCA
    GATGCCTCCA GCTCCACGGC AACAGCAGCT GATGCAGCCA CAGCCTTCGC TAAAAAGTTT
    GAGGTGTTGT TCTGCGGGCG AGTGGTGGTG GCCCATAAGA AAGCACCTCC TGCACTAATA
    GATGAGTGTA TAGAGAAGTT TGGACGTGTC AGTGTTACAG GCTCACTTGC AGCGGGCATC
    AGGCGGGCGT TTTCACTCAA TCAGGTGGTG AATGGAACAA ATGAGGGTGA TGGAGGAGGA
    AAGCGACCTT TGTTTTTTAA AAGAGATCAA AGTTTTCCAT TTCTGCAGGC ATTGGATGAG
    AACGGCCTCT CACCGGAGCT CCAGCGACGG GCAGCAGACG GAGCCACTGG CGTTCAACCT
    ACCAGTCTAC AGGAGAACAG AACCATGCTG TTTACAATTG GGCGATCTCA GATCTTTCTC
    GTGAGTCCTG ACACTAAGAA AGTGGCCATA GAGAAGAGCT TCAGAGAGAT TTCCTTCTGT
    TCTCAGGGAA TTCGTCACGT GGACCACTTC GGCTTCATAT GCAGGGAGTC GGTTGAATGC
    GGAGGCTGCC AGTTTGTTTG CTATGTTTTC CAGTGTGCAA ACGAGGCTCT GGTGGATGAG
    ATCATGTTGA CTTTGAAGCA GGCGTTCTCT GTGGCCGCTC TCCAGCAGAA CAGCAGGACC
    CAGAGTCAGC AGTGTGACGG CTGTCCCCTG CAGCAGCTGC ACAAACTCTG TGACAGAATA
    GAGGGTCTTC ATCCATCCAA AGCTAAGCTG GAACTGCAGA AGCATTTGGC AGATCTGGAT
    AATCAGGAGC AGGCAGCTGT GTTTGAGAGC ACCATGAAAG CCAGGCCTAA ATCGGATCAG
    GAGGAAAATG AGTTGATTTT CTCGTGTCTA AGACGGATGT ACGAGGACAG ACAAAAGACA
    CACACACACA ACGGGCAAAC AAAAAAGGAT GTTGTTGTGA ATGCTGAAAG CAGCGTGAGT
    TCTAGCCGTG TGCGTCTGGA GGAGTTCAAG TCTAGAGCTA AAAGATCCCT CACTGAGTCA
    CTGGAGGGGA TCTGGAAGGG TGGCAATAAG TCAAAATCCC AGAGGGAAAA CAGTGAAGGA
    GAGAGCAACT CCTCCAGTTC TACCACACTG AGCAGCACAC ATCAGGAAAC ATCATTGCAG
    GAGCCACTCT GCTCTCTGCT TCTCTCCAGT CCGCTGCGCT CCCACAATTC CACAGGGAAT
    CTGAAGCATC TTGAAGGCTC TGACAGGGGT CAGGAGGATC CTGAGAGTCC GCCTCAGGGT
    TTCAGGAGAC GCGCAAGCAC TATCAGTCAC TCTCCCATGC TGTCGTCCTC TGAGCCGAGC
    CTCCCTCCTC TACAGCCCAC TGCTGCCAAC AAACCCAAAC TGGTCCGACA CTACTCCGTC
    AGCACAGACT CCCCACACCA GAGCAATCCT TCCTCCTCTC CGGCTTCTTC CTCTCCTGTG
    TGTCAGCGGC CGCTGAGGCC TCGTTCTTTT GCTCTTGATC ATTCCACACG GCCTCTCAAG
    CGCAGTCCAT CAGGGCTGAG TGGCTTTAGA GCGAGATTAC ACTCGTCCTC CTCTGTTCCA
    AATTTCCTCA AATTTCTGGC CCCTGTAGTG GAAAGTGATG ATACCTGTGA CTCTCAGAGG
    AAGAGTGATG TTGCGGCCCT TTCCAGTCCT GGATGTGTTA TTGGTGATGA CAGTCCATTG
    CGCAACCGGA GACACTCATG GAGACAACAG ATCTTCTTAA GAGTCGCTAC ACCACAGAAG
    GGCTCGGACG CCAAAGAATC CACGTTAGAG GTTGGCAGCA GAATGGCACT GGGAGGTGGA
    GTTGGTGGCG GAGACAAAAC ACTTGGGGCG GTGCCAGAGG AGAGGAGAAA GAGGAGCGGG
    GCGGAGCTTA GGAAGTTATG GAGGAAAGCC ATTCTTCAAC AGATACTCCT ACTGCGGATG
    GAGAAAGAGA ATCAGAAACT ACAAGCGTCA GAGAGTGATC TGCAGTGCAA GCGACAGAAG
    CTGGATTATG AAGAGATCAC TCCATGCCTG AAAGATGTCA CTCTGGTCTG GGAGAGGATG
    CTCGGCACGG CTGGAAGAGC AAAGGTCAGA TTTGACATGG ATGAGATCCA CGGTGCTGTG
    GGACAAGGCG TTCCTCGTCA GCATCGAGGA GAGATCTGGA AGTTTCTCTC TGAGCAGTAT
    TTGCTGAGGC AGGAGGTGCC CAGTGCCAAA CCGCCAAACA ATGACACTCC TTACAAAGAG
    CTACTGAAGC AGCTCACATC CCAGCAGCAT GCCATCCTCA TTGACTTGGG TCGTACATTT
    CCCACTCACC CGTATTTCAG CGCTCAGCTT GGTGCAGGCC AGCTGTCCCT CTATAACCTA
    CTGAAGGCAT ATTCTCTGCT GGACCCGGAG GTCGGTTACT GCCAGGGCCT GAGCTTTGTG
    GCTGGTGTTT TGCTGCTCCA CATGAGCGAG GAAGACGCGT TTCACATGCT CAAATTCCTC
    ATGTACGACA TGGGGCTCAG GAAACAGTAC AGGCCTGATA TGATCATACT GCAGATTCAG
    ATGTATCAGC TGTCTCGTCT GTTGCATGAC TACCACAGGG AGCTCTACTC TCATCTGGAG
    CTTTATGAGA TCGGCCCCAG TCTCTACGCC GCTCCATGGT TCCTCACCGC CTTTGCCTCA
    CACTTCCCGC TCGGATTTGT GGCAAGAGTA TTCGACATGC TGTTTCTTCA GGGGTCAGAG
    GTCATCTTCA AAGTGGCCTT GAGTCTTCTC GGGAGCCACA AACCTCTGAT TCTCCAACAC
    GACAATCTTG AGGCAATCGT GGAGTTCATC AAAACAACCT TACCCAACCT CGGCCTCGTG
    CAAATGGAGA AAACTATCAA TCAGGTGTCA GAGATGGATA TCAGTAAGCA GCTGCAGGCC
    TATGAAGTGG AATATCATGT TCTTCAGGAT GAGCTTTTGG ATGGTCCCTC CACACTTAGC
    CATTCCCAGC GAGCGGCCCA GTTGGAGAAG ACCAATGGAA GTCTCCGACA ACAAAACTTA
    GACCTCCTAG AGGAGCTACA GGTTGCCCAC GCTAAAATCA GATGTTTAGA GACTCGTGTG
    GACGGCCTTG TGAACCGAGA GGCTGAGCTG CAATCACAAG TAAAGACTCT CCAGCAGGAA
    CAAACAGATC TCCAGCAGAC CGTATGCAAA CTCAGAGACC TTCTCAGCAG TCTGGGCATA
    AGCAACCCCT GCCCAGACCT CCAAATCCTC ACAAACAACA ACCAATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.