depdc5 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

The following depdc5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the depdc5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OXa48894 XM_031892202.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $391.30
$559.00
OXa42156 XM_018090138.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa42156 XM_018090136.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
OXa48893 XM_031892178.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa48893 XM_031892183.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa48893 XM_031892191.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $727.30
$1039.00
OXa42155 XM_018090128.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OXa48887 XM_004910591.4
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa48888 XM_031892165.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa48888 XM_031892148.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa48889 XM_002931918.5
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa48890 XM_031892156.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa48891 XM_031892162.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa48892 XM_031892174.1
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $811.30
$1159.00
OXa03643 XM_004910591.3
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa03644 XM_002931918.4
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa37170 XM_012969655.2
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa37171 XM_012969676.2
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa37172 XM_012969661.2
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa37173 XM_012969662.2
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa37174 XM_012969665.2
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OXa37175 XM_012969680.2
Latest version!
Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OXa48894
    Clone ID Related Accession (Same CDS sequence) XM_031892202.1
    Accession Version XM_031892202.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2259bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCAACGAGAT GAAGAGGGAG CTCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748062.1
    CDS306..2564
    Translation

    Target ORF information:

    RefSeq Version XM_031892202.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892202.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCAACGAGAT GAAGAGGGAG CTCAGATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42156
    Clone ID Related Accession (Same CDS sequence) XM_018090138.1 , XM_018090136.1
    Accession Version XM_018090138.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATTTCT GGCTGATCTC TTTAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCC
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCTT TTTGGGCATT TTGGAGATTT
    CTTCTTTTCC TCCAGTCGCT GCCTTGGCAA ACCATAGAAG AGGGAAAGAA GGCAGTGCGA
    GAGACACAAG TTCGAAAAAG TTCTTGTTCC TATGACACAT CCTGTAGCCC AGTCAGTCGG
    CCATTTACAG CAGAGGAAGG CCGTAGCCAA AATTCAGATG AGAGCTCTTT GGGAAAACTC
    TCCAACATCC TACTTATCCC AAGGCCACAT CTGCATCAAT ATGAGGCCAG CAGCTCTTTG
    GGTTATACTA GCACAAGAGA TTTCTTGGAG AGGATGATTG AATCCCAACA GCGAGACTCA
    AGTGCTCCTG GCAGATTCCA TGTGGGCAGT GCAGAATCCA TGTTACACGT CCGACCTGGG
    GGATACCACC CGCAGAGAGC ACTCATCAAC CCATTTGCAC CTTCACGTAT GCCAATGAAA
    CTGACATCAA ATCGCAGGAG GTGGATGCAC ACATTTCCAG TGGGCCCATC TGGAGAAGCA
    ATACAAATAC ATCACCAAAC TCGTCAGAAT ATGGCAGAGC TACAGGGTAG TGGCATGCGT
    GAGGCATCTC ATTCTTCTGC AGAGTTGCTA GAATTGGCTT ACCATGAAGC CACTGGCAGA
    CACAGTGGCC CTCGCACCCC TGGGGAAAAT GGCTCTTGTG GGAGCAATGA AGAATTCTCC
    TGCAACACAC CCATCAATGG GCAGAGGAAC CACAATACAG GGTCTAATCA CAGGGAGAAA
    GAGTCCTCTG ATGACAGCGT ACCATGCTGC CCAGAACCCA TTTTGATGCT GTCTGCTCCC
    CCCGTAGTGC CTGGTTTCTG CTGCACGGTT GGGGTGGATT GGAAGTCCCT CACCACCCCA
    GCCTGCCTGC CGCTTACCAC AGACTACTTC CCAGATAGGC AAAGTCTGCA AAATGATTAC
    ACAGAGGGAT GCTATGACCT TCTGCCAGAA GCTGACCTTG ACAGACGAGA TGAAGAGGGA
    GCTCAGATGA CTGCACAACA AGTTTTTGAA GAGTTTATCT GCCAACGTCT TATGCAAGGT
    TATCAAATTA TTGTTCAGCC AAAACACCCA AAAGCCATCT CCTCTGTTCC TCCTCCACTC
    AGCAGCAGCC CGCTTTACAG AAGAGGGCTG GTGTCAAGGA ACAGAGCTGA AGAGGAGAAC
    CAGTACTGGC TGAGTATGGG CCGAACCTTC CATAAGGTGA CACTAAAGGA CAAGATAATA
    ACAGTCACTA GATATCTACC CAAGTATCCA TATGAATCTG CCCAAATCAG CTACATGTAC
    AGTCTGTGCC CATCACACTC TGAGTGTGAT TTTGTGTCCA GCTGGGTGGA ATTCTCCCAT
    GAGCGACTGG AGGATTATAA ATGGAACTAT TTGGATCAGT ATATATGCTC AGCTGGCTCT
    GAAGATTTCA GTCTCATCGA ATCCCTAAAG TTTTGGCGCA CACGTTTCCT TTTGCTCCCT
    TCTTGTGTGG GACCCACAAA ACGCATCACT GAGGGGGAAC AACGCTGTGA TATATATGGT
    GAACGCCCTC GATCAGAACA AGAGGAAGCA CAGTTGTTAG ACGGCTTTTT ACGCTTTGTG
    GAGGGGCTAA ACCGAATCCG TCGACGCCAC CGTTCTGATC GCATGATCCG GAAAGGAGCA
    GGTATGAAGG GCCTGCAATT GCCTTCTCAT CCGCCAGAAC CTCCAGGTCC ACCTATAGGG
    AAGAAAGGAA CATCAGCACT ATCAGCATTG CTTGAGATGG AAGCTAATCA AAAGAGCCTG
    GGGGAGCAGC AAGCCCTTGT ACATGCTGCA AAATTGTCAT CTCAGGTCTC AGACCCTGCG
    GTAACTCCCA CTTATGTGGA CAGTCCTCGT AAGGATGGGG CCTTCTTTGT GGAGTTTGTA
    CGCAGTCCAC GCACAGCGCC TTCCTTCACT CCTCAGATCT CCATTGACCA AGGAACTTCT
    TTAGTGACTG ATGGAAATAA TGCCATGCCT AACCCTACCC CTTCTGAAAG AATACAGAGT
    CAGGGTGCAA CCTCTGTAGA TCAGCCCCCC ACTTCAACTG AATGCAGTGC ACAGCAGATA
    TCTGTGGGTC CAAGTGTATT ACCAACCACT GCCAGTCTGA GTGAGATTCT GGAGGCTATG
    AAACATCCAA CTACAGGCAT CCCTCTCCTG TCAGAGCAGA AGTGCCTTCC TCCTGGTTGC
    TTTATCAGTG CTGAGGCTGT GCATTGGCTG GTCAATAATA TTAGCTGGGT GCAAAGTCAG
    GTTATGGCCA TGGATATCAT GCAGAAGATG CTTGAAGAGC AGTATATCAC ACATGCGTCA
    GGAGAGACAT TTAGAACTTT CATCTATGGC TTTTACTTGT ACAAGATCCC AGCTGACAAG
    GAGCCTGACA GAGCATCAAA CCTGGTACAG CCATGCGTGT GGCATTCCAC AGCCATGGAG
    GATTTCACAG CCTTCCAACG GAAGTGGTTT GAGGTTGCAT TGGTGGAGGA AGAGCTACTG
    CACTCCGAGA TCCCACCTTT TCTTTTGCCG TGGTTACCTA GTCGCCCCGC ATCTTATGCA
    AGTAGGCACA GCTCCTTTAG CCGCAGCTTC GGAGGAAGGA GCCAGGCCGC TGCTCTGCTA
    GCTGCTACAG TGCCAGAGCA GAGAACGGTC ACTTTAGATG TGGATGTTAA TAACCGCACT
    GACCGGCTAG AATGGTGTAG CAGCTACTAT CACGGAAACT TCTCCTTGAC TGCTGCTTTT
    GAGATCAAAC TGCACTGGAT GGCAGCAACT GCAGCAATGC TATTTGAGAT GGTTCAAGGA
    TGGCACAGAA AAGCTCCGTC CTGCGGTTTC CTCCTAGTAC CTGTTGTAGA AGGCCCTTTT
    TCTCTCCCTA GTTACTTGTA TGGGGATCCT TTGCGTGCTC AGCTTTTTAT TCCACTTAAT
    ATCAGCTGCT TAGTAAAAGC TGAGAATGAC CATCTTTTTG AAGGTTTTGA ACCAGAAACT
    TACTGGGAAC GAATGTACTT GCTACAGGAA GTAATAGCTT ATAGATTTGG CTTTATTCAG
    GACAAATATT CAGCATCTGC ATTTAACTTC CCATCAGAGA ATAAACCACA GTATATACAT
    GTGACAGGTA CTGTATTCTT ACAGCTGCCT TACTCAAAGC GGAAAGGACA GCAGAGACGT
    AGGAGGAATT CGACCAGCTC TTCTACTCAG AACCTTTTTG GAGAAGAACG CATAGGGTAT
    AATTGGGCCT ACAATACCAT GCTGACTAAA ACGTGGCGCT CCATTGGTAC AGGAGATGAG
    AGGTTTGCTG ACCGACTGCT TAAAGACTTT ACTGACTTCT GTGCCAACAG AGATAACAGG
    TTAGGCACAT TCTGGGCAAA CTGCTTGGAG AAAATGAACA CAAGTGCGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945627.1
    CDS653..4786
    Translation

    Target ORF information:

    RefSeq Version XM_018090138.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090138.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATTTCT GGCTGATCTC TTTAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCC
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCTT TTTGGGCATT TTGGAGATTT
    CTTCTTTTCC TCCAGTCGCT GCCTTGGCAA ACCATAGAAG AGGGAAAGAA GGCAGTGCGA
    GAGACACAAG TTCGAAAAAG TTCTTGTTCC TATGACACAT CCTGTAGCCC AGTCAGTCGG
    CCATTTACAG CAGAGGAAGG CCGTAGCCAA AATTCAGATG AGAGCTCTTT GGGAAAACTC
    TCCAACATCC TACTTATCCC AAGGCCACAT CTGCATCAAT ATGAGGCCAG CAGCTCTTTG
    GGTTATACTA GCACAAGAGA TTTCTTGGAG AGGATGATTG AATCCCAACA GCGAGACTCA
    AGTGCTCCTG GCAGATTCCA TGTGGGCAGT GCAGAATCCA TGTTACACGT CCGACCTGGG
    GGATACCACC CGCAGAGAGC ACTCATCAAC CCATTTGCAC CTTCACGTAT GCCAATGAAA
    CTGACATCAA ATCGCAGGAG GTGGATGCAC ACATTTCCAG TGGGCCCATC TGGAGAAGCA
    ATACAAATAC ATCACCAAAC TCGTCAGAAT ATGGCAGAGC TACAGGGTAG TGGCATGCGT
    GAGGCATCTC ATTCTTCTGC AGAGTTGCTA GAATTGGCTT ACCATGAAGC CACTGGCAGA
    CACAGTGGCC CTCGCACCCC TGGGGAAAAT GGCTCTTGTG GGAGCAATGA AGAATTCTCC
    TGCAACACAC CCATCAATGG GCAGAGGAAC CACAATACAG GGTCTAATCA CAGGGAGAAA
    GAGTCCTCTG ATGACAGCGT ACCATGCTGC CCAGAACCCA TTTTGATGCT GTCTGCTCCC
    CCCGTAGTGC CTGGTTTCTG CTGCACGGTT GGGGTGGATT GGAAGTCCCT CACCACCCCA
    GCCTGCCTGC CGCTTACCAC AGACTACTTC CCAGATAGGC AAAGTCTGCA AAATGATTAC
    ACAGAGGGAT GCTATGACCT TCTGCCAGAA GCTGACCTTG ACAGACGAGA TGAAGAGGGA
    GCTCAGATGA CTGCACAACA AGTTTTTGAA GAGTTTATCT GCCAACGTCT TATGCAAGGT
    TATCAAATTA TTGTTCAGCC AAAACACCCA AAAGCCATCT CCTCTGTTCC TCCTCCACTC
    AGCAGCAGCC CGCTTTACAG AAGAGGGCTG GTGTCAAGGA ACAGAGCTGA AGAGGAGAAC
    CAGTACTGGC TGAGTATGGG CCGAACCTTC CATAAGGTGA CACTAAAGGA CAAGATAATA
    ACAGTCACTA GATATCTACC CAAGTATCCA TATGAATCTG CCCAAATCAG CTACATGTAC
    AGTCTGTGCC CATCACACTC TGAGTGTGAT TTTGTGTCCA GCTGGGTGGA ATTCTCCCAT
    GAGCGACTGG AGGATTATAA ATGGAACTAT TTGGATCAGT ATATATGCTC AGCTGGCTCT
    GAAGATTTCA GTCTCATCGA ATCCCTAAAG TTTTGGCGCA CACGTTTCCT TTTGCTCCCT
    TCTTGTGTGG GACCCACAAA ACGCATCACT GAGGGGGAAC AACGCTGTGA TATATATGGT
    GAACGCCCTC GATCAGAACA AGAGGAAGCA CAGTTGTTAG ACGGCTTTTT ACGCTTTGTG
    GAGGGGCTAA ACCGAATCCG TCGACGCCAC CGTTCTGATC GCATGATCCG GAAAGGAGCA
    GGTATGAAGG GCCTGCAATT GCCTTCTCAT CCGCCAGAAC CTCCAGGTCC ACCTATAGGG
    AAGAAAGGAA CATCAGCACT ATCAGCATTG CTTGAGATGG AAGCTAATCA AAAGAGCCTG
    GGGGAGCAGC AAGCCCTTGT ACATGCTGCA AAATTGTCAT CTCAGGTCTC AGACCCTGCG
    GTAACTCCCA CTTATGTGGA CAGTCCTCGT AAGGATGGGG CCTTCTTTGT GGAGTTTGTA
    CGCAGTCCAC GCACAGCGCC TTCCTTCACT CCTCAGATCT CCATTGACCA AGGAACTTCT
    TTAGTGACTG ATGGAAATAA TGCCATGCCT AACCCTACCC CTTCTGAAAG AATACAGAGT
    CAGGGTGCAA CCTCTGTAGA TCAGCCCCCC ACTTCAACTG AATGCAGTGC ACAGCAGATA
    TCTGTGGGTC CAAGTGTATT ACCAACCACT GCCAGTCTGA GTGAGATTCT GGAGGCTATG
    AAACATCCAA CTACAGGCAT CCCTCTCCTG TCAGAGCAGA AGTGCCTTCC TCCTGGTTGC
    TTTATCAGTG CTGAGGCTGT GCATTGGCTG GTCAATAATA TTAGCTGGGT GCAAAGTCAG
    GTTATGGCCA TGGATATCAT GCAGAAGATG CTTGAAGAGC AGTATATCAC ACATGCGTCA
    GGAGAGACAT TTAGAACTTT CATCTATGGC TTTTACTTGT ACAAGATCCC AGCTGACAAG
    GAGCCTGACA GAGCATCAAA CCTGGTACAG CCATGCGTGT GGCATTCCAC AGCCATGGAG
    GATTTCACAG CCTTCCAACG GAAGTGGTTT GAGGTTGCAT TGGTGGAGGA AGAGCTACTG
    CACTCCGAGA TCCCACCTTT TCTTTTGCCG TGGTTACCTA GTCGCCCCGC ATCTTATGCA
    AGTAGGCACA GCTCCTTTAG CCGCAGCTTC GGAGGAAGGA GCCAGGCCGC TGCTCTGCTA
    GCTGCTACAG TGCCAGAGCA GAGAACGGTC ACTTTAGATG TGGATGTTAA TAACCGCACT
    GACCGGCTAG AATGGTGTAG CAGCTACTAT CACGGAAACT TCTCCTTGAC TGCTGCTTTT
    GAGATCAAAC TGCACTGGAT GGCAGCAACT GCAGCAATGC TATTTGAGAT GGTTCAAGGA
    TGGCACAGAA AAGCTCCGTC CTGCGGTTTC CTCCTAGTAC CTGTTGTAGA AGGCCCTTTT
    TCTCTCCCTA GTTACTTGTA TGGGGATCCT TTGCGTGCTC AGCTTTTTAT TCCACTTAAT
    ATCAGCTGCT TAGTAAAAGC TGAGAATGAC CATCTTTTTG AAGGTTTTGA ACCAGAAACT
    TACTGGGAAC GAATGTACTT GCTACAGGAA GTAATAGCTT ATAGATTTGG CTTTATTCAG
    GACAAATATT CAGCATCTGC ATTTAACTTC CCATCAGAGA ATAAACCACA GTATATACAT
    GTGACAGGTA CTGTATTCTT ACAGCTGCCT TACTCAAAGC GGAAAGGACA GCAGAGACGT
    AGGAGGAATT CGACCAGCTC TTCTACTCAG AACCTTTTTG GAGAAGAACG CATAGGGTAT
    AATTGGGCCT ACAATACCAT GCTGACTAAA ACGTGGCGCT CCATTGGTAC AGGAGATGAG
    AGGTTTGCTG ACCGACTGCT TAAAGACTTT ACTGACTTCT GTGCCAACAG AGATAACAGG
    TTAGGCACAT TCTGGGCAAA CTGCTTGGAG AAAATGAACA CAAGTGCGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42156
    Clone ID Related Accession (Same CDS sequence) XM_018090138.1 , XM_018090136.1
    Accession Version XM_018090136.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4134bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATTTCT GGCTGATCTC TTTAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCC
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCTT TTTGGGCATT TTGGAGATTT
    CTTCTTTTCC TCCAGTCGCT GCCTTGGCAA ACCATAGAAG AGGGAAAGAA GGCAGTGCGA
    GAGACACAAG TTCGAAAAAG TTCTTGTTCC TATGACACAT CCTGTAGCCC AGTCAGTCGG
    CCATTTACAG CAGAGGAAGG CCGTAGCCAA AATTCAGATG AGAGCTCTTT GGGAAAACTC
    TCCAACATCC TACTTATCCC AAGGCCACAT CTGCATCAAT ATGAGGCCAG CAGCTCTTTG
    GGTTATACTA GCACAAGAGA TTTCTTGGAG AGGATGATTG AATCCCAACA GCGAGACTCA
    AGTGCTCCTG GCAGATTCCA TGTGGGCAGT GCAGAATCCA TGTTACACGT CCGACCTGGG
    GGATACCACC CGCAGAGAGC ACTCATCAAC CCATTTGCAC CTTCACGTAT GCCAATGAAA
    CTGACATCAA ATCGCAGGAG GTGGATGCAC ACATTTCCAG TGGGCCCATC TGGAGAAGCA
    ATACAAATAC ATCACCAAAC TCGTCAGAAT ATGGCAGAGC TACAGGGTAG TGGCATGCGT
    GAGGCATCTC ATTCTTCTGC AGAGTTGCTA GAATTGGCTT ACCATGAAGC CACTGGCAGA
    CACAGTGGCC CTCGCACCCC TGGGGAAAAT GGCTCTTGTG GGAGCAATGA AGAATTCTCC
    TGCAACACAC CCATCAATGG GCAGAGGAAC CACAATACAG GGTCTAATCA CAGGGAGAAA
    GAGTCCTCTG ATGACAGCGT ACCATGCTGC CCAGAACCCA TTTTGATGCT GTCTGCTCCC
    CCCGTAGTGC CTGGTTTCTG CTGCACGGTT GGGGTGGATT GGAAGTCCCT CACCACCCCA
    GCCTGCCTGC CGCTTACCAC AGACTACTTC CCAGATAGGC AAAGTCTGCA AAATGATTAC
    ACAGAGGGAT GCTATGACCT TCTGCCAGAA GCTGACCTTG ACAGACGAGA TGAAGAGGGA
    GCTCAGATGA CTGCACAACA AGTTTTTGAA GAGTTTATCT GCCAACGTCT TATGCAAGGT
    TATCAAATTA TTGTTCAGCC AAAACACCCA AAAGCCATCT CCTCTGTTCC TCCTCCACTC
    AGCAGCAGCC CGCTTTACAG AAGAGGGCTG GTGTCAAGGA ACAGAGCTGA AGAGGAGAAC
    CAGTACTGGC TGAGTATGGG CCGAACCTTC CATAAGGTGA CACTAAAGGA CAAGATAATA
    ACAGTCACTA GATATCTACC CAAGTATCCA TATGAATCTG CCCAAATCAG CTACATGTAC
    AGTCTGTGCC CATCACACTC TGAGTGTGAT TTTGTGTCCA GCTGGGTGGA ATTCTCCCAT
    GAGCGACTGG AGGATTATAA ATGGAACTAT TTGGATCAGT ATATATGCTC AGCTGGCTCT
    GAAGATTTCA GTCTCATCGA ATCCCTAAAG TTTTGGCGCA CACGTTTCCT TTTGCTCCCT
    TCTTGTGTGG GACCCACAAA ACGCATCACT GAGGGGGAAC AACGCTGTGA TATATATGGT
    GAACGCCCTC GATCAGAACA AGAGGAAGCA CAGTTGTTAG ACGGCTTTTT ACGCTTTGTG
    GAGGGGCTAA ACCGAATCCG TCGACGCCAC CGTTCTGATC GCATGATCCG GAAAGGAGCA
    GGTATGAAGG GCCTGCAATT GCCTTCTCAT CCGCCAGAAC CTCCAGGTCC ACCTATAGGG
    AAGAAAGGAA CATCAGCACT ATCAGCATTG CTTGAGATGG AAGCTAATCA AAAGAGCCTG
    GGGGAGCAGC AAGCCCTTGT ACATGCTGCA AAATTGTCAT CTCAGGTCTC AGACCCTGCG
    GTAACTCCCA CTTATGTGGA CAGTCCTCGT AAGGATGGGG CCTTCTTTGT GGAGTTTGTA
    CGCAGTCCAC GCACAGCGCC TTCCTTCACT CCTCAGATCT CCATTGACCA AGGAACTTCT
    TTAGTGACTG ATGGAAATAA TGCCATGCCT AACCCTACCC CTTCTGAAAG AATACAGAGT
    CAGGGTGCAA CCTCTGTAGA TCAGCCCCCC ACTTCAACTG AATGCAGTGC ACAGCAGATA
    TCTGTGGGTC CAAGTGTATT ACCAACCACT GCCAGTCTGA GTGAGATTCT GGAGGCTATG
    AAACATCCAA CTACAGGCAT CCCTCTCCTG TCAGAGCAGA AGTGCCTTCC TCCTGGTTGC
    TTTATCAGTG CTGAGGCTGT GCATTGGCTG GTCAATAATA TTAGCTGGGT GCAAAGTCAG
    GTTATGGCCA TGGATATCAT GCAGAAGATG CTTGAAGAGC AGTATATCAC ACATGCGTCA
    GGAGAGACAT TTAGAACTTT CATCTATGGC TTTTACTTGT ACAAGATCCC AGCTGACAAG
    GAGCCTGACA GAGCATCAAA CCTGGTACAG CCATGCGTGT GGCATTCCAC AGCCATGGAG
    GATTTCACAG CCTTCCAACG GAAGTGGTTT GAGGTTGCAT TGGTGGAGGA AGAGCTACTG
    CACTCCGAGA TCCCACCTTT TCTTTTGCCG TGGTTACCTA GTCGCCCCGC ATCTTATGCA
    AGTAGGCACA GCTCCTTTAG CCGCAGCTTC GGAGGAAGGA GCCAGGCCGC TGCTCTGCTA
    GCTGCTACAG TGCCAGAGCA GAGAACGGTC ACTTTAGATG TGGATGTTAA TAACCGCACT
    GACCGGCTAG AATGGTGTAG CAGCTACTAT CACGGAAACT TCTCCTTGAC TGCTGCTTTT
    GAGATCAAAC TGCACTGGAT GGCAGCAACT GCAGCAATGC TATTTGAGAT GGTTCAAGGA
    TGGCACAGAA AAGCTCCGTC CTGCGGTTTC CTCCTAGTAC CTGTTGTAGA AGGCCCTTTT
    TCTCTCCCTA GTTACTTGTA TGGGGATCCT TTGCGTGCTC AGCTTTTTAT TCCACTTAAT
    ATCAGCTGCT TAGTAAAAGC TGAGAATGAC CATCTTTTTG AAGGTTTTGA ACCAGAAACT
    TACTGGGAAC GAATGTACTT GCTACAGGAA GTAATAGCTT ATAGATTTGG CTTTATTCAG
    GACAAATATT CAGCATCTGC ATTTAACTTC CCATCAGAGA ATAAACCACA GTATATACAT
    GTGACAGGTA CTGTATTCTT ACAGCTGCCT TACTCAAAGC GGAAAGGACA GCAGAGACGT
    AGGAGGAATT CGACCAGCTC TTCTACTCAG AACCTTTTTG GAGAAGAACG CATAGGGTAT
    AATTGGGCCT ACAATACCAT GCTGACTAAA ACGTGGCGCT CCATTGGTAC AGGAGATGAG
    AGGTTTGCTG ACCGACTGCT TAAAGACTTT ACTGACTTCT GTGCCAACAG AGATAACAGG
    TTAGGCACAT TCTGGGCAAA CTGCTTGGAG AAAATGAACA CAAGTGCGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945625.1
    CDS641..4774
    Translation

    Target ORF information:

    RefSeq Version XM_018090136.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090136.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    ATGGATTTCT GGCTGATCTC TTTAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCC
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCTT TTTGGGCATT TTGGAGATTT
    CTTCTTTTCC TCCAGTCGCT GCCTTGGCAA ACCATAGAAG AGGGAAAGAA GGCAGTGCGA
    GAGACACAAG TTCGAAAAAG TTCTTGTTCC TATGACACAT CCTGTAGCCC AGTCAGTCGG
    CCATTTACAG CAGAGGAAGG CCGTAGCCAA AATTCAGATG AGAGCTCTTT GGGAAAACTC
    TCCAACATCC TACTTATCCC AAGGCCACAT CTGCATCAAT ATGAGGCCAG CAGCTCTTTG
    GGTTATACTA GCACAAGAGA TTTCTTGGAG AGGATGATTG AATCCCAACA GCGAGACTCA
    AGTGCTCCTG GCAGATTCCA TGTGGGCAGT GCAGAATCCA TGTTACACGT CCGACCTGGG
    GGATACCACC CGCAGAGAGC ACTCATCAAC CCATTTGCAC CTTCACGTAT GCCAATGAAA
    CTGACATCAA ATCGCAGGAG GTGGATGCAC ACATTTCCAG TGGGCCCATC TGGAGAAGCA
    ATACAAATAC ATCACCAAAC TCGTCAGAAT ATGGCAGAGC TACAGGGTAG TGGCATGCGT
    GAGGCATCTC ATTCTTCTGC AGAGTTGCTA GAATTGGCTT ACCATGAAGC CACTGGCAGA
    CACAGTGGCC CTCGCACCCC TGGGGAAAAT GGCTCTTGTG GGAGCAATGA AGAATTCTCC
    TGCAACACAC CCATCAATGG GCAGAGGAAC CACAATACAG GGTCTAATCA CAGGGAGAAA
    GAGTCCTCTG ATGACAGCGT ACCATGCTGC CCAGAACCCA TTTTGATGCT GTCTGCTCCC
    CCCGTAGTGC CTGGTTTCTG CTGCACGGTT GGGGTGGATT GGAAGTCCCT CACCACCCCA
    GCCTGCCTGC CGCTTACCAC AGACTACTTC CCAGATAGGC AAAGTCTGCA AAATGATTAC
    ACAGAGGGAT GCTATGACCT TCTGCCAGAA GCTGACCTTG ACAGACGAGA TGAAGAGGGA
    GCTCAGATGA CTGCACAACA AGTTTTTGAA GAGTTTATCT GCCAACGTCT TATGCAAGGT
    TATCAAATTA TTGTTCAGCC AAAACACCCA AAAGCCATCT CCTCTGTTCC TCCTCCACTC
    AGCAGCAGCC CGCTTTACAG AAGAGGGCTG GTGTCAAGGA ACAGAGCTGA AGAGGAGAAC
    CAGTACTGGC TGAGTATGGG CCGAACCTTC CATAAGGTGA CACTAAAGGA CAAGATAATA
    ACAGTCACTA GATATCTACC CAAGTATCCA TATGAATCTG CCCAAATCAG CTACATGTAC
    AGTCTGTGCC CATCACACTC TGAGTGTGAT TTTGTGTCCA GCTGGGTGGA ATTCTCCCAT
    GAGCGACTGG AGGATTATAA ATGGAACTAT TTGGATCAGT ATATATGCTC AGCTGGCTCT
    GAAGATTTCA GTCTCATCGA ATCCCTAAAG TTTTGGCGCA CACGTTTCCT TTTGCTCCCT
    TCTTGTGTGG GACCCACAAA ACGCATCACT GAGGGGGAAC AACGCTGTGA TATATATGGT
    GAACGCCCTC GATCAGAACA AGAGGAAGCA CAGTTGTTAG ACGGCTTTTT ACGCTTTGTG
    GAGGGGCTAA ACCGAATCCG TCGACGCCAC CGTTCTGATC GCATGATCCG GAAAGGAGCA
    GGTATGAAGG GCCTGCAATT GCCTTCTCAT CCGCCAGAAC CTCCAGGTCC ACCTATAGGG
    AAGAAAGGAA CATCAGCACT ATCAGCATTG CTTGAGATGG AAGCTAATCA AAAGAGCCTG
    GGGGAGCAGC AAGCCCTTGT ACATGCTGCA AAATTGTCAT CTCAGGTCTC AGACCCTGCG
    GTAACTCCCA CTTATGTGGA CAGTCCTCGT AAGGATGGGG CCTTCTTTGT GGAGTTTGTA
    CGCAGTCCAC GCACAGCGCC TTCCTTCACT CCTCAGATCT CCATTGACCA AGGAACTTCT
    TTAGTGACTG ATGGAAATAA TGCCATGCCT AACCCTACCC CTTCTGAAAG AATACAGAGT
    CAGGGTGCAA CCTCTGTAGA TCAGCCCCCC ACTTCAACTG AATGCAGTGC ACAGCAGATA
    TCTGTGGGTC CAAGTGTATT ACCAACCACT GCCAGTCTGA GTGAGATTCT GGAGGCTATG
    AAACATCCAA CTACAGGCAT CCCTCTCCTG TCAGAGCAGA AGTGCCTTCC TCCTGGTTGC
    TTTATCAGTG CTGAGGCTGT GCATTGGCTG GTCAATAATA TTAGCTGGGT GCAAAGTCAG
    GTTATGGCCA TGGATATCAT GCAGAAGATG CTTGAAGAGC AGTATATCAC ACATGCGTCA
    GGAGAGACAT TTAGAACTTT CATCTATGGC TTTTACTTGT ACAAGATCCC AGCTGACAAG
    GAGCCTGACA GAGCATCAAA CCTGGTACAG CCATGCGTGT GGCATTCCAC AGCCATGGAG
    GATTTCACAG CCTTCCAACG GAAGTGGTTT GAGGTTGCAT TGGTGGAGGA AGAGCTACTG
    CACTCCGAGA TCCCACCTTT TCTTTTGCCG TGGTTACCTA GTCGCCCCGC ATCTTATGCA
    AGTAGGCACA GCTCCTTTAG CCGCAGCTTC GGAGGAAGGA GCCAGGCCGC TGCTCTGCTA
    GCTGCTACAG TGCCAGAGCA GAGAACGGTC ACTTTAGATG TGGATGTTAA TAACCGCACT
    GACCGGCTAG AATGGTGTAG CAGCTACTAT CACGGAAACT TCTCCTTGAC TGCTGCTTTT
    GAGATCAAAC TGCACTGGAT GGCAGCAACT GCAGCAATGC TATTTGAGAT GGTTCAAGGA
    TGGCACAGAA AAGCTCCGTC CTGCGGTTTC CTCCTAGTAC CTGTTGTAGA AGGCCCTTTT
    TCTCTCCCTA GTTACTTGTA TGGGGATCCT TTGCGTGCTC AGCTTTTTAT TCCACTTAAT
    ATCAGCTGCT TAGTAAAAGC TGAGAATGAC CATCTTTTTG AAGGTTTTGA ACCAGAAACT
    TACTGGGAAC GAATGTACTT GCTACAGGAA GTAATAGCTT ATAGATTTGG CTTTATTCAG
    GACAAATATT CAGCATCTGC ATTTAACTTC CCATCAGAGA ATAAACCACA GTATATACAT
    GTGACAGGTA CTGTATTCTT ACAGCTGCCT TACTCAAAGC GGAAAGGACA GCAGAGACGT
    AGGAGGAATT CGACCAGCTC TTCTACTCAG AACCTTTTTG GAGAAGAACG CATAGGGTAT
    AATTGGGCCT ACAATACCAT GCTGACTAAA ACGTGGCGCT CCATTGGTAC AGGAGATGAG
    AGGTTTGCTG ACCGACTGCT TAAAGACTTT ACTGACTTCT GTGCCAACAG AGATAACAGG
    TTAGGCACAT TCTGGGCAAA CTGCTTGGAG AAAATGAACA CAAGTGCGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48893
    Clone ID Related Accession (Same CDS sequence) XM_031892178.1 , XM_031892183.1 , XM_031892191.1
    Accession Version XM_031892178.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTCT GGCTGATCTC TTCAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCA
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCAG TGCGAGAGAC ACAAGTTCGA
    AAAAGTTCTT GTTCCTATGA CACATCCTGT AGCCCAGTCA GTCGGCCATT TACAGCAGAG
    GAAGGCCGTA GCCAAAATTC AGATGAGAGC TCTTTGGGAA AACTCTCCAA CATCCTACTT
    ATCCCAAGGC CACATCTGCA TCAATATGAG GCCAGCAGCT CTTTGGGTTA TACTAGCACA
    AGAGATTTCT TGGAGAGGAT GATTGAATCC CAACAGCGAG ACTCAAGTGC TCCTGGCAGA
    TTCCATGTGG GCAGTGCAGA ATCCATGTTA CACGTCCGAC CTGGGGGATA CCACCCGCAG
    AGAGCACTCA TCAACCCATT TGCACCTTCA CGTATGCCAA TGAAACTGAC ATCAAATCGC
    AGGAGGTGGA TGCACACATT TCCAGTGGGC CCATCTGGAG AAGCAATACA AATACATCAC
    CAAACTCGTC AGAATATGGC AGAGCTACAG GGTAGTGGCA TGCGTGAGGC ATCTCATTCT
    TCTGCAGAGT TGCTAGAATT GGCTTACCAT GAAGCCACTG GCAGACACAG TGGCCCTCGC
    ACCCCTGGGG AAAATGGCTC TTGTGGGAGC AATGAAGAAT TCTCCTGCAA CACACCCATC
    AATGGGCAGA GGAACCACAA TACAGGGTCT AATCACAGGG AGAAAGAGTC CTCTGATGAC
    AGCGTACCAT GCTGCCCAGA ACCCATTTTG ATGCTGTCTG CTCCCCCCGT AGTGCCTGGT
    TTCTGCTGCA CGGTTGGGGT GGATTGGAAG TCCCTCACCA CCCCAGCCTG CCTGCCGCTT
    ACCACAGACT ACTTCCCAGA TAGGCAAAGT CTACAAAATG ATTACACAGA GGGATGCTAT
    GACCTTCTGC CAGAAGCTGA CCTTGACAGA CGAGATGAAG AGGGAGCTCA GATGACTGCA
    CAACAAGTTT TTGAAGAGTT TATCTGCCAA CGTCTTATGC AAGGTTATCA AATTATTGTT
    CAGCCAAAAC ACCCAAAAGC CATCTCCTCT GTTCCTCCTC CACTCAGCAG CAGCCCGCTT
    TACAGAAGAG GGCTGGTGTC AAGGAACAGA GCTGAAGAGG AGAACCAGTA CTGGCTGAGT
    ATGGGCCGAA CCTTCCATAA GGTGACACTA AAGGACAAGA TAATAACAGT CACTAGATAT
    CTACCCAAGT ATCCATATGA ATCTGCCCAA ATCAGCTACA TGTACAGTCT GTGCCCATCA
    CACTCTGAGT GTGATTTTGT GTCCAGCTGG GTGGAATTCT CCCATGAGCG ACTGGAGGAT
    TATAAATGGA ACTATTTGGA TCAGTATATA TGCTCAGCTG GCTCTGAAGA TTTCAGTCTC
    ATCGAATCCC TAAAGTTTTG GCGCACACGT TTCCTTTTGC TCCCTTCTTG TGTGGGACCC
    ACAAAACGCA TCACTGAGGG GGAACAACGC TGTGATATAT ATGGTGAACG CCCTCGATCA
    GAACAAGAGG AAGCACAGTT GTTAGACGGC TTTTTACGCT TTGTGGAGGG GCTAAACCGA
    ATCCGTCGAC GCCACCGTTC TGATCGCATG ATCCGGAAAG GAGCAGGTAT GAAGGGCCTG
    CAATTGCCTT CTCATCCGCC AGAACCTCCA GGTCCACCTA TAGGGAAGAA AGGAACATCA
    GCACTATCAG CATTGCTTGA GATGGAAGCT AATCAAAAGA GCCTGGGGGA GCAGCAAGCC
    CTTGTACATG CTGCAAAATT GTCATCTCAG GTCTCAGACC CTGCGGTAAC TCCCACTTAT
    GTGGACAGTC CTCGTAAGGA TGGGGCCTTC TTTGTGGAGT TTGTACGCAG TCCACGCACA
    GCGCCTTCCT TCACTCCTCA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CTTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGCAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAATATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748038.1
    CDS641..4699
    Translation

    Target ORF information:

    RefSeq Version XM_031892178.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892178.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTCT GGCTGATCTC TTCAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCA
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCAG TGCGAGAGAC ACAAGTTCGA
    AAAAGTTCTT GTTCCTATGA CACATCCTGT AGCCCAGTCA GTCGGCCATT TACAGCAGAG
    GAAGGCCGTA GCCAAAATTC AGATGAGAGC TCTTTGGGAA AACTCTCCAA CATCCTACTT
    ATCCCAAGGC CACATCTGCA TCAATATGAG GCCAGCAGCT CTTTGGGTTA TACTAGCACA
    AGAGATTTCT TGGAGAGGAT GATTGAATCC CAACAGCGAG ACTCAAGTGC TCCTGGCAGA
    TTCCATGTGG GCAGTGCAGA ATCCATGTTA CACGTCCGAC CTGGGGGATA CCACCCGCAG
    AGAGCACTCA TCAACCCATT TGCACCTTCA CGTATGCCAA TGAAACTGAC ATCAAATCGC
    AGGAGGTGGA TGCACACATT TCCAGTGGGC CCATCTGGAG AAGCAATACA AATACATCAC
    CAAACTCGTC AGAATATGGC AGAGCTACAG GGTAGTGGCA TGCGTGAGGC ATCTCATTCT
    TCTGCAGAGT TGCTAGAATT GGCTTACCAT GAAGCCACTG GCAGACACAG TGGCCCTCGC
    ACCCCTGGGG AAAATGGCTC TTGTGGGAGC AATGAAGAAT TCTCCTGCAA CACACCCATC
    AATGGGCAGA GGAACCACAA TACAGGGTCT AATCACAGGG AGAAAGAGTC CTCTGATGAC
    AGCGTACCAT GCTGCCCAGA ACCCATTTTG ATGCTGTCTG CTCCCCCCGT AGTGCCTGGT
    TTCTGCTGCA CGGTTGGGGT GGATTGGAAG TCCCTCACCA CCCCAGCCTG CCTGCCGCTT
    ACCACAGACT ACTTCCCAGA TAGGCAAAGT CTACAAAATG ATTACACAGA GGGATGCTAT
    GACCTTCTGC CAGAAGCTGA CCTTGACAGA CGAGATGAAG AGGGAGCTCA GATGACTGCA
    CAACAAGTTT TTGAAGAGTT TATCTGCCAA CGTCTTATGC AAGGTTATCA AATTATTGTT
    CAGCCAAAAC ACCCAAAAGC CATCTCCTCT GTTCCTCCTC CACTCAGCAG CAGCCCGCTT
    TACAGAAGAG GGCTGGTGTC AAGGAACAGA GCTGAAGAGG AGAACCAGTA CTGGCTGAGT
    ATGGGCCGAA CCTTCCATAA GGTGACACTA AAGGACAAGA TAATAACAGT CACTAGATAT
    CTACCCAAGT ATCCATATGA ATCTGCCCAA ATCAGCTACA TGTACAGTCT GTGCCCATCA
    CACTCTGAGT GTGATTTTGT GTCCAGCTGG GTGGAATTCT CCCATGAGCG ACTGGAGGAT
    TATAAATGGA ACTATTTGGA TCAGTATATA TGCTCAGCTG GCTCTGAAGA TTTCAGTCTC
    ATCGAATCCC TAAAGTTTTG GCGCACACGT TTCCTTTTGC TCCCTTCTTG TGTGGGACCC
    ACAAAACGCA TCACTGAGGG GGAACAACGC TGTGATATAT ATGGTGAACG CCCTCGATCA
    GAACAAGAGG AAGCACAGTT GTTAGACGGC TTTTTACGCT TTGTGGAGGG GCTAAACCGA
    ATCCGTCGAC GCCACCGTTC TGATCGCATG ATCCGGAAAG GAGCAGGTAT GAAGGGCCTG
    CAATTGCCTT CTCATCCGCC AGAACCTCCA GGTCCACCTA TAGGGAAGAA AGGAACATCA
    GCACTATCAG CATTGCTTGA GATGGAAGCT AATCAAAAGA GCCTGGGGGA GCAGCAAGCC
    CTTGTACATG CTGCAAAATT GTCATCTCAG GTCTCAGACC CTGCGGTAAC TCCCACTTAT
    GTGGACAGTC CTCGTAAGGA TGGGGCCTTC TTTGTGGAGT TTGTACGCAG TCCACGCACA
    GCGCCTTCCT TCACTCCTCA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CTTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGCAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAATATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48893
    Clone ID Related Accession (Same CDS sequence) XM_031892178.1 , XM_031892183.1 , XM_031892191.1
    Accession Version XM_031892183.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTCT GGCTGATCTC TTCAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCA
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCAG TGCGAGAGAC ACAAGTTCGA
    AAAAGTTCTT GTTCCTATGA CACATCCTGT AGCCCAGTCA GTCGGCCATT TACAGCAGAG
    GAAGGCCGTA GCCAAAATTC AGATGAGAGC TCTTTGGGAA AACTCTCCAA CATCCTACTT
    ATCCCAAGGC CACATCTGCA TCAATATGAG GCCAGCAGCT CTTTGGGTTA TACTAGCACA
    AGAGATTTCT TGGAGAGGAT GATTGAATCC CAACAGCGAG ACTCAAGTGC TCCTGGCAGA
    TTCCATGTGG GCAGTGCAGA ATCCATGTTA CACGTCCGAC CTGGGGGATA CCACCCGCAG
    AGAGCACTCA TCAACCCATT TGCACCTTCA CGTATGCCAA TGAAACTGAC ATCAAATCGC
    AGGAGGTGGA TGCACACATT TCCAGTGGGC CCATCTGGAG AAGCAATACA AATACATCAC
    CAAACTCGTC AGAATATGGC AGAGCTACAG GGTAGTGGCA TGCGTGAGGC ATCTCATTCT
    TCTGCAGAGT TGCTAGAATT GGCTTACCAT GAAGCCACTG GCAGACACAG TGGCCCTCGC
    ACCCCTGGGG AAAATGGCTC TTGTGGGAGC AATGAAGAAT TCTCCTGCAA CACACCCATC
    AATGGGCAGA GGAACCACAA TACAGGGTCT AATCACAGGG AGAAAGAGTC CTCTGATGAC
    AGCGTACCAT GCTGCCCAGA ACCCATTTTG ATGCTGTCTG CTCCCCCCGT AGTGCCTGGT
    TTCTGCTGCA CGGTTGGGGT GGATTGGAAG TCCCTCACCA CCCCAGCCTG CCTGCCGCTT
    ACCACAGACT ACTTCCCAGA TAGGCAAAGT CTACAAAATG ATTACACAGA GGGATGCTAT
    GACCTTCTGC CAGAAGCTGA CCTTGACAGA CGAGATGAAG AGGGAGCTCA GATGACTGCA
    CAACAAGTTT TTGAAGAGTT TATCTGCCAA CGTCTTATGC AAGGTTATCA AATTATTGTT
    CAGCCAAAAC ACCCAAAAGC CATCTCCTCT GTTCCTCCTC CACTCAGCAG CAGCCCGCTT
    TACAGAAGAG GGCTGGTGTC AAGGAACAGA GCTGAAGAGG AGAACCAGTA CTGGCTGAGT
    ATGGGCCGAA CCTTCCATAA GGTGACACTA AAGGACAAGA TAATAACAGT CACTAGATAT
    CTACCCAAGT ATCCATATGA ATCTGCCCAA ATCAGCTACA TGTACAGTCT GTGCCCATCA
    CACTCTGAGT GTGATTTTGT GTCCAGCTGG GTGGAATTCT CCCATGAGCG ACTGGAGGAT
    TATAAATGGA ACTATTTGGA TCAGTATATA TGCTCAGCTG GCTCTGAAGA TTTCAGTCTC
    ATCGAATCCC TAAAGTTTTG GCGCACACGT TTCCTTTTGC TCCCTTCTTG TGTGGGACCC
    ACAAAACGCA TCACTGAGGG GGAACAACGC TGTGATATAT ATGGTGAACG CCCTCGATCA
    GAACAAGAGG AAGCACAGTT GTTAGACGGC TTTTTACGCT TTGTGGAGGG GCTAAACCGA
    ATCCGTCGAC GCCACCGTTC TGATCGCATG ATCCGGAAAG GAGCAGGTAT GAAGGGCCTG
    CAATTGCCTT CTCATCCGCC AGAACCTCCA GGTCCACCTA TAGGGAAGAA AGGAACATCA
    GCACTATCAG CATTGCTTGA GATGGAAGCT AATCAAAAGA GCCTGGGGGA GCAGCAAGCC
    CTTGTACATG CTGCAAAATT GTCATCTCAG GTCTCAGACC CTGCGGTAAC TCCCACTTAT
    GTGGACAGTC CTCGTAAGGA TGGGGCCTTC TTTGTGGAGT TTGTACGCAG TCCACGCACA
    GCGCCTTCCT TCACTCCTCA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CTTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGCAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAATATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748043.1
    CDS648..4706
    Translation

    Target ORF information:

    RefSeq Version XM_031892183.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892183.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTCT GGCTGATCTC TTCAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCA
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCAG TGCGAGAGAC ACAAGTTCGA
    AAAAGTTCTT GTTCCTATGA CACATCCTGT AGCCCAGTCA GTCGGCCATT TACAGCAGAG
    GAAGGCCGTA GCCAAAATTC AGATGAGAGC TCTTTGGGAA AACTCTCCAA CATCCTACTT
    ATCCCAAGGC CACATCTGCA TCAATATGAG GCCAGCAGCT CTTTGGGTTA TACTAGCACA
    AGAGATTTCT TGGAGAGGAT GATTGAATCC CAACAGCGAG ACTCAAGTGC TCCTGGCAGA
    TTCCATGTGG GCAGTGCAGA ATCCATGTTA CACGTCCGAC CTGGGGGATA CCACCCGCAG
    AGAGCACTCA TCAACCCATT TGCACCTTCA CGTATGCCAA TGAAACTGAC ATCAAATCGC
    AGGAGGTGGA TGCACACATT TCCAGTGGGC CCATCTGGAG AAGCAATACA AATACATCAC
    CAAACTCGTC AGAATATGGC AGAGCTACAG GGTAGTGGCA TGCGTGAGGC ATCTCATTCT
    TCTGCAGAGT TGCTAGAATT GGCTTACCAT GAAGCCACTG GCAGACACAG TGGCCCTCGC
    ACCCCTGGGG AAAATGGCTC TTGTGGGAGC AATGAAGAAT TCTCCTGCAA CACACCCATC
    AATGGGCAGA GGAACCACAA TACAGGGTCT AATCACAGGG AGAAAGAGTC CTCTGATGAC
    AGCGTACCAT GCTGCCCAGA ACCCATTTTG ATGCTGTCTG CTCCCCCCGT AGTGCCTGGT
    TTCTGCTGCA CGGTTGGGGT GGATTGGAAG TCCCTCACCA CCCCAGCCTG CCTGCCGCTT
    ACCACAGACT ACTTCCCAGA TAGGCAAAGT CTACAAAATG ATTACACAGA GGGATGCTAT
    GACCTTCTGC CAGAAGCTGA CCTTGACAGA CGAGATGAAG AGGGAGCTCA GATGACTGCA
    CAACAAGTTT TTGAAGAGTT TATCTGCCAA CGTCTTATGC AAGGTTATCA AATTATTGTT
    CAGCCAAAAC ACCCAAAAGC CATCTCCTCT GTTCCTCCTC CACTCAGCAG CAGCCCGCTT
    TACAGAAGAG GGCTGGTGTC AAGGAACAGA GCTGAAGAGG AGAACCAGTA CTGGCTGAGT
    ATGGGCCGAA CCTTCCATAA GGTGACACTA AAGGACAAGA TAATAACAGT CACTAGATAT
    CTACCCAAGT ATCCATATGA ATCTGCCCAA ATCAGCTACA TGTACAGTCT GTGCCCATCA
    CACTCTGAGT GTGATTTTGT GTCCAGCTGG GTGGAATTCT CCCATGAGCG ACTGGAGGAT
    TATAAATGGA ACTATTTGGA TCAGTATATA TGCTCAGCTG GCTCTGAAGA TTTCAGTCTC
    ATCGAATCCC TAAAGTTTTG GCGCACACGT TTCCTTTTGC TCCCTTCTTG TGTGGGACCC
    ACAAAACGCA TCACTGAGGG GGAACAACGC TGTGATATAT ATGGTGAACG CCCTCGATCA
    GAACAAGAGG AAGCACAGTT GTTAGACGGC TTTTTACGCT TTGTGGAGGG GCTAAACCGA
    ATCCGTCGAC GCCACCGTTC TGATCGCATG ATCCGGAAAG GAGCAGGTAT GAAGGGCCTG
    CAATTGCCTT CTCATCCGCC AGAACCTCCA GGTCCACCTA TAGGGAAGAA AGGAACATCA
    GCACTATCAG CATTGCTTGA GATGGAAGCT AATCAAAAGA GCCTGGGGGA GCAGCAAGCC
    CTTGTACATG CTGCAAAATT GTCATCTCAG GTCTCAGACC CTGCGGTAAC TCCCACTTAT
    GTGGACAGTC CTCGTAAGGA TGGGGCCTTC TTTGTGGAGT TTGTACGCAG TCCACGCACA
    GCGCCTTCCT TCACTCCTCA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CTTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGCAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAATATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48893
    Clone ID Related Accession (Same CDS sequence) XM_031892178.1 , XM_031892183.1 , XM_031892191.1
    Accession Version XM_031892191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4059bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTCT GGCTGATCTC TTCAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCA
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCAG TGCGAGAGAC ACAAGTTCGA
    AAAAGTTCTT GTTCCTATGA CACATCCTGT AGCCCAGTCA GTCGGCCATT TACAGCAGAG
    GAAGGCCGTA GCCAAAATTC AGATGAGAGC TCTTTGGGAA AACTCTCCAA CATCCTACTT
    ATCCCAAGGC CACATCTGCA TCAATATGAG GCCAGCAGCT CTTTGGGTTA TACTAGCACA
    AGAGATTTCT TGGAGAGGAT GATTGAATCC CAACAGCGAG ACTCAAGTGC TCCTGGCAGA
    TTCCATGTGG GCAGTGCAGA ATCCATGTTA CACGTCCGAC CTGGGGGATA CCACCCGCAG
    AGAGCACTCA TCAACCCATT TGCACCTTCA CGTATGCCAA TGAAACTGAC ATCAAATCGC
    AGGAGGTGGA TGCACACATT TCCAGTGGGC CCATCTGGAG AAGCAATACA AATACATCAC
    CAAACTCGTC AGAATATGGC AGAGCTACAG GGTAGTGGCA TGCGTGAGGC ATCTCATTCT
    TCTGCAGAGT TGCTAGAATT GGCTTACCAT GAAGCCACTG GCAGACACAG TGGCCCTCGC
    ACCCCTGGGG AAAATGGCTC TTGTGGGAGC AATGAAGAAT TCTCCTGCAA CACACCCATC
    AATGGGCAGA GGAACCACAA TACAGGGTCT AATCACAGGG AGAAAGAGTC CTCTGATGAC
    AGCGTACCAT GCTGCCCAGA ACCCATTTTG ATGCTGTCTG CTCCCCCCGT AGTGCCTGGT
    TTCTGCTGCA CGGTTGGGGT GGATTGGAAG TCCCTCACCA CCCCAGCCTG CCTGCCGCTT
    ACCACAGACT ACTTCCCAGA TAGGCAAAGT CTACAAAATG ATTACACAGA GGGATGCTAT
    GACCTTCTGC CAGAAGCTGA CCTTGACAGA CGAGATGAAG AGGGAGCTCA GATGACTGCA
    CAACAAGTTT TTGAAGAGTT TATCTGCCAA CGTCTTATGC AAGGTTATCA AATTATTGTT
    CAGCCAAAAC ACCCAAAAGC CATCTCCTCT GTTCCTCCTC CACTCAGCAG CAGCCCGCTT
    TACAGAAGAG GGCTGGTGTC AAGGAACAGA GCTGAAGAGG AGAACCAGTA CTGGCTGAGT
    ATGGGCCGAA CCTTCCATAA GGTGACACTA AAGGACAAGA TAATAACAGT CACTAGATAT
    CTACCCAAGT ATCCATATGA ATCTGCCCAA ATCAGCTACA TGTACAGTCT GTGCCCATCA
    CACTCTGAGT GTGATTTTGT GTCCAGCTGG GTGGAATTCT CCCATGAGCG ACTGGAGGAT
    TATAAATGGA ACTATTTGGA TCAGTATATA TGCTCAGCTG GCTCTGAAGA TTTCAGTCTC
    ATCGAATCCC TAAAGTTTTG GCGCACACGT TTCCTTTTGC TCCCTTCTTG TGTGGGACCC
    ACAAAACGCA TCACTGAGGG GGAACAACGC TGTGATATAT ATGGTGAACG CCCTCGATCA
    GAACAAGAGG AAGCACAGTT GTTAGACGGC TTTTTACGCT TTGTGGAGGG GCTAAACCGA
    ATCCGTCGAC GCCACCGTTC TGATCGCATG ATCCGGAAAG GAGCAGGTAT GAAGGGCCTG
    CAATTGCCTT CTCATCCGCC AGAACCTCCA GGTCCACCTA TAGGGAAGAA AGGAACATCA
    GCACTATCAG CATTGCTTGA GATGGAAGCT AATCAAAAGA GCCTGGGGGA GCAGCAAGCC
    CTTGTACATG CTGCAAAATT GTCATCTCAG GTCTCAGACC CTGCGGTAAC TCCCACTTAT
    GTGGACAGTC CTCGTAAGGA TGGGGCCTTC TTTGTGGAGT TTGTACGCAG TCCACGCACA
    GCGCCTTCCT TCACTCCTCA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CTTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGCAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAATATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748051.1
    CDS71..4129
    Translation

    Target ORF information:

    RefSeq Version XM_031892191.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGGATTTCT GGCTGATCTC TTCAACAAAT GGAAGGACAA GAACTGCAGT CACGAGGTTA 
    CAGTGGTTCT ATTCTCTAGG ACCTTTTATG ATGCAAAGAA TATTGAGGAC TTTCCAGAAG
    CACACCGTAA TGCGTCCGTC ATGCAAGATC ACAAGGGCAG ATTCTATGAA GACTTCTACA
    AAGGGCTTTC CCCCAGGGCA TAATTCTACT TCAGATCAAG GAAATTATCT AGAGGCCATT
    AACCTGTCCT TTAATGTGTT TGACAAGCAT TATATAAATC GCAACTTTGA CCGAACTGGT
    CAGATGTCTG TTGTGATCAC TCCAGGTGTT GGTGTTTTCC AAGTGGATCG TTCACTAATG
    ATTCTGACCA AGCAGCGAAT GATTGATAAT GGGATTGGTG TGGATCTGGT ATGTATGGGA
    GAGCAGCCGC TTCATGCTGT TCCACTTTTC AAGCTGCACA ATCGTTCCAG TCCAGGAGAT
    TCACGTCTTG GAGATGATTA TAACATCCCT CACTGGATTA ACCATAGCTT CTACACATCG
    CAGAGCCAGC CTCTAAATAA CAGCTTTACC CCACGCATCA AACTGGCACC TCGCAAGACA
    AATAGTGAGA AAGGAAGGAC TGGAAAAGAC CCATCCATAG GAAGCCCCAA AGAGACAGAG
    AATGCTCTTC CCATACAAGT AGACTACGAT GCTTATGACT CACAGGTCTT CCGATTACCA
    GGTCCATCCA GGGCACAGCG AAGCATAACT TTCAGGGCAG TGCGAGAGAC ACAAGTTCGA
    AAAAGTTCTT GTTCCTATGA CACATCCTGT AGCCCAGTCA GTCGGCCATT TACAGCAGAG
    GAAGGCCGTA GCCAAAATTC AGATGAGAGC TCTTTGGGAA AACTCTCCAA CATCCTACTT
    ATCCCAAGGC CACATCTGCA TCAATATGAG GCCAGCAGCT CTTTGGGTTA TACTAGCACA
    AGAGATTTCT TGGAGAGGAT GATTGAATCC CAACAGCGAG ACTCAAGTGC TCCTGGCAGA
    TTCCATGTGG GCAGTGCAGA ATCCATGTTA CACGTCCGAC CTGGGGGATA CCACCCGCAG
    AGAGCACTCA TCAACCCATT TGCACCTTCA CGTATGCCAA TGAAACTGAC ATCAAATCGC
    AGGAGGTGGA TGCACACATT TCCAGTGGGC CCATCTGGAG AAGCAATACA AATACATCAC
    CAAACTCGTC AGAATATGGC AGAGCTACAG GGTAGTGGCA TGCGTGAGGC ATCTCATTCT
    TCTGCAGAGT TGCTAGAATT GGCTTACCAT GAAGCCACTG GCAGACACAG TGGCCCTCGC
    ACCCCTGGGG AAAATGGCTC TTGTGGGAGC AATGAAGAAT TCTCCTGCAA CACACCCATC
    AATGGGCAGA GGAACCACAA TACAGGGTCT AATCACAGGG AGAAAGAGTC CTCTGATGAC
    AGCGTACCAT GCTGCCCAGA ACCCATTTTG ATGCTGTCTG CTCCCCCCGT AGTGCCTGGT
    TTCTGCTGCA CGGTTGGGGT GGATTGGAAG TCCCTCACCA CCCCAGCCTG CCTGCCGCTT
    ACCACAGACT ACTTCCCAGA TAGGCAAAGT CTACAAAATG ATTACACAGA GGGATGCTAT
    GACCTTCTGC CAGAAGCTGA CCTTGACAGA CGAGATGAAG AGGGAGCTCA GATGACTGCA
    CAACAAGTTT TTGAAGAGTT TATCTGCCAA CGTCTTATGC AAGGTTATCA AATTATTGTT
    CAGCCAAAAC ACCCAAAAGC CATCTCCTCT GTTCCTCCTC CACTCAGCAG CAGCCCGCTT
    TACAGAAGAG GGCTGGTGTC AAGGAACAGA GCTGAAGAGG AGAACCAGTA CTGGCTGAGT
    ATGGGCCGAA CCTTCCATAA GGTGACACTA AAGGACAAGA TAATAACAGT CACTAGATAT
    CTACCCAAGT ATCCATATGA ATCTGCCCAA ATCAGCTACA TGTACAGTCT GTGCCCATCA
    CACTCTGAGT GTGATTTTGT GTCCAGCTGG GTGGAATTCT CCCATGAGCG ACTGGAGGAT
    TATAAATGGA ACTATTTGGA TCAGTATATA TGCTCAGCTG GCTCTGAAGA TTTCAGTCTC
    ATCGAATCCC TAAAGTTTTG GCGCACACGT TTCCTTTTGC TCCCTTCTTG TGTGGGACCC
    ACAAAACGCA TCACTGAGGG GGAACAACGC TGTGATATAT ATGGTGAACG CCCTCGATCA
    GAACAAGAGG AAGCACAGTT GTTAGACGGC TTTTTACGCT TTGTGGAGGG GCTAAACCGA
    ATCCGTCGAC GCCACCGTTC TGATCGCATG ATCCGGAAAG GAGCAGGTAT GAAGGGCCTG
    CAATTGCCTT CTCATCCGCC AGAACCTCCA GGTCCACCTA TAGGGAAGAA AGGAACATCA
    GCACTATCAG CATTGCTTGA GATGGAAGCT AATCAAAAGA GCCTGGGGGA GCAGCAAGCC
    CTTGTACATG CTGCAAAATT GTCATCTCAG GTCTCAGACC CTGCGGTAAC TCCCACTTAT
    GTGGACAGTC CTCGTAAGGA TGGGGCCTTC TTTGTGGAGT TTGTACGCAG TCCACGCACA
    GCGCCTTCCT TCACTCCTCA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CTTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGCAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAATATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa42155
    Clone ID Related Accession (Same CDS sequence) XM_018090128.1
    Accession Version XM_018090128.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGTT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GGATGGGGCC TTCTTTGTGG AGTTTGTACG CAGTCCACGC
    ACAGCGCCTT CCTTCACTCC TCAGATCTCC ATTGACCAAG GAACTTCTTT AGTGACTGAT
    GGAAATAATG CCATGCCTAA CCCTACCCCT TCTGAAAGAA TACAGAGTCA GGGTGCAACC
    TCTGTAGATC AGCCCCCCAC TTCAACTGAA TGCAGTGCAC AGCAGATATC TGTGGGTCCA
    AGTGTATTAC CAACCACTGC CAGTCTGAGT GAGATTCTGG AGGCTATGAA ACATCCAACT
    ACAGGCATCC CTCTCCTGTC AGAGCAGAAG TGCCTTCCTC CTGGTTGCTT TATCAGTGCT
    GAGGCTGTGC ATTGGCTGGT CAATAATATT AGCTGGGTGC AAAGTCAGGT TATGGCCATG
    GATATCATGC AGAAGATGCT TGAAGAGCAG TATATCACAC ATGCGTCAGG AGAGACATTT
    AGAACTTTCA TCTATGGCTT TTACTTGTAC AAGATCCCAG CTGACAAGGA GCCTGACAGA
    GCATCAAACC TGGTACAGCC ATGCGTGTGG CATTCCACAG CCATGGAGGA TTTCACAGCC
    TTCCAACGGA AGTGGTTTGA GGTTGCATTG GTGGAGGAAG AGCTACTGCA CTCCGAGATC
    CCACCTTTTC TTTTGCCGTG GTTACCTAGT CGCCCCGCAT CTTATGCAAG TAGGCACAGC
    TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC CAGGCCGCTG CTCTGCTAGC TGCTACAGTG
    CCAGAGCAGA GAACGGTCAC TTTAGATGTG GATGTTAATA ACCGCACTGA CCGGCTAGAA
    TGGTGTAGCA GCTACTATCA CGGAAACTTC TCCTTGACTG CTGCTTTTGA GATCAAACTG
    CACTGGATGG CAGCAACTGC AGCAATGCTA TTTGAGATGG TTCAAGGATG GCACAGAAAA
    GCTCCGTCCT GCGGTTTCCT CCTAGTACCT GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT
    TACTTGTATG GGGATCCTTT GCGTGCTCAG CTTTTTATTC CACTTAATAT CAGCTGCTTA
    GTAAAAGCTG AGAATGACCA TCTTTTTGAA GGTTTTGAAC CAGAAACTTA CTGGGAACGA
    ATGTACTTGC TACAGGAAGT AATAGCTTAT AGATTTGGCT TTATTCAGGA CAAATATTCA
    GCATCTGCAT TTAACTTCCC ATCAGAGAAT AAACCACAGT ATATACATGT GACAGGTACT
    GTATTCTTAC AGCTGCCTTA CTCAAAGCGG AAAGGACAGC AGAGACGTAG GAGGAATTCG
    ACCAGCTCTT CTACTCAGAA CCTTTTTGGA GAAGAACGCA TAGGGTATAA TTGGGCCTAC
    AATACCATGC TGACTAAAAC GTGGCGCTCC ATTGGTACAG GAGATGAGAG GTTTGCTGAC
    CGACTGCTTA AAGACTTTAC TGACTTCTGT GCCAACAGAG ATAACAGGTT AGGCACATTC
    TGGGCAAACT GCTTGGAGAA AATGAACACA AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017945617.1
    CDS48..4889
    Translation

    Target ORF information:

    RefSeq Version XM_018090128.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_018090128.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGTT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GGATGGGGCC TTCTTTGTGG AGTTTGTACG CAGTCCACGC
    ACAGCGCCTT CCTTCACTCC TCAGATCTCC ATTGACCAAG GAACTTCTTT AGTGACTGAT
    GGAAATAATG CCATGCCTAA CCCTACCCCT TCTGAAAGAA TACAGAGTCA GGGTGCAACC
    TCTGTAGATC AGCCCCCCAC TTCAACTGAA TGCAGTGCAC AGCAGATATC TGTGGGTCCA
    AGTGTATTAC CAACCACTGC CAGTCTGAGT GAGATTCTGG AGGCTATGAA ACATCCAACT
    ACAGGCATCC CTCTCCTGTC AGAGCAGAAG TGCCTTCCTC CTGGTTGCTT TATCAGTGCT
    GAGGCTGTGC ATTGGCTGGT CAATAATATT AGCTGGGTGC AAAGTCAGGT TATGGCCATG
    GATATCATGC AGAAGATGCT TGAAGAGCAG TATATCACAC ATGCGTCAGG AGAGACATTT
    AGAACTTTCA TCTATGGCTT TTACTTGTAC AAGATCCCAG CTGACAAGGA GCCTGACAGA
    GCATCAAACC TGGTACAGCC ATGCGTGTGG CATTCCACAG CCATGGAGGA TTTCACAGCC
    TTCCAACGGA AGTGGTTTGA GGTTGCATTG GTGGAGGAAG AGCTACTGCA CTCCGAGATC
    CCACCTTTTC TTTTGCCGTG GTTACCTAGT CGCCCCGCAT CTTATGCAAG TAGGCACAGC
    TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC CAGGCCGCTG CTCTGCTAGC TGCTACAGTG
    CCAGAGCAGA GAACGGTCAC TTTAGATGTG GATGTTAATA ACCGCACTGA CCGGCTAGAA
    TGGTGTAGCA GCTACTATCA CGGAAACTTC TCCTTGACTG CTGCTTTTGA GATCAAACTG
    CACTGGATGG CAGCAACTGC AGCAATGCTA TTTGAGATGG TTCAAGGATG GCACAGAAAA
    GCTCCGTCCT GCGGTTTCCT CCTAGTACCT GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT
    TACTTGTATG GGGATCCTTT GCGTGCTCAG CTTTTTATTC CACTTAATAT CAGCTGCTTA
    GTAAAAGCTG AGAATGACCA TCTTTTTGAA GGTTTTGAAC CAGAAACTTA CTGGGAACGA
    ATGTACTTGC TACAGGAAGT AATAGCTTAT AGATTTGGCT TTATTCAGGA CAAATATTCA
    GCATCTGCAT TTAACTTCCC ATCAGAGAAT AAACCACAGT ATATACATGT GACAGGTACT
    GTATTCTTAC AGCTGCCTTA CTCAAAGCGG AAAGGACAGC AGAGACGTAG GAGGAATTCG
    ACCAGCTCTT CTACTCAGAA CCTTTTTGGA GAAGAACGCA TAGGGTATAA TTGGGCCTAC
    AATACCATGC TGACTAAAAC GTGGCGCTCC ATTGGTACAG GAGATGAGAG GTTTGCTGAC
    CGACTGCTTA AAGACTTTAC TGACTTCTGT GCCAACAGAG ATAACAGGTT AGGCACATTC
    TGGGCAAACT GCTTGGAGAA AATGAACACA AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48887
    Clone ID Related Accession (Same CDS sequence) XM_004910591.4
    Accession Version XM_004910591.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_004910591.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT ACAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCT TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAAGTAGGC ACAGCTCCTT TAGCCGCAGC
    TTCGGAGGAA GGAGCCAGGC CGCTGCTCTG CTAGCTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG CAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAATATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910648.1
    CDS306..5132
    Translation

    Target ORF information:

    RefSeq Version XM_004910591.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910591.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT ACAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCT TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAAGTAGGC ACAGCTCCTT TAGCCGCAGC
    TTCGGAGGAA GGAGCCAGGC CGCTGCTCTG CTAGCTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG CAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAATATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48888
    Clone ID Related Accession (Same CDS sequence) XM_031892165.1 , XM_031892148.1
    Accession Version XM_031892165.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGGACCA ACAGAGTATA CAAGCTAGTC ATCCACAAGA AAGGCTTTGG GGGCAGTGAT 
    GATGAGTTGG TGATAAACCC CAAGGTCTTT CCATATATTA ACCTGGGGGA CATTGTTGAA
    ATTGCTCACC CAAATGATGA ATACAGTCCC CTTCTCCTGC AGGTGAAGTC ACTAAAAGAA
    GATTTGCAAA AAGAAACTGT TAGTATTGAC CAAACCGTAG CCCAAGTGTT TCGCCTACGG
    CCATACCAGG ATGTTTATGT CAATGTTGTT GACCCTAAGG ATGTGACTCT TGATCTTGTG
    GAGTTAACAT TTAAGGACCA GTATATAGGT CGTGGAGATA TGTGGAGGCT AAAAAAGAGT
    TTGGTCAGCT CCTGTGCTTA CATAACTCAG AAAGTGGAGT TTGCTGGAAT TCGAGCACAA
    GTTGGAGAAT TATGGGTTAA GAGCGAGAAA GTCACCTGTG GGTACATCAG TGAAGACACA
    CGAGTTGTAT TCAGGTCCAC ATCGGCCATG GTGTATATAT TTATACAAAT GAGCAGTGAA
    CTGTGGGACT TTGATATCTA TGGTGATCTG TATTTTGAGA AGGCAGTAAA TGGATTTCTG
    GCTGATCTCT TCAACAAATG GAAGGACAAG AACTGCAGTC ACGAGGTTAC AGTGGTTCTA
    TTCTCTAGGA CCTTTTATGA TGCAAAGAAT ATTGAGGACT TTCCAGAAGC ACACCGTAAT
    GCGTCCGTCA TGCAAGATCA CAAGGGCAGA TTCTATGAAG ACTTCTACAA GGTGGTTGTA
    CAAAATGAAA GGCGAGAAGA ATGGACTTCA TTGCTAGTCA CTATTAAGAA ACTTTTTATT
    CAGTACCCAG TGCTTGTACG ACTGGAAAAT GCAGAGGGCT TTCCCCCAGG GCATAATTCT
    ACTTCAGATC AAGGAAATTA TCTAGAGGCC ATTAACCTGT CCTTTAATGT GTTTGACAAG
    CATTATATAA ATCGCAACTT TGACCGAACT GGTCAGATGT CTGTTGTGAT CACTCCAGGT
    GTTGGTGTTT TCCAAGTGGA TCGTTCACTA ATGATTCTGA CCAAGCAGCG AATGATTGAT
    AATGGGATTG GTGTGGATCT GGTATGTATG GGAGAGCAGC CGCTTCATGC TGTTCCACTT
    TTCAAGCTGC ACAATCGTTC CAGTCCAGGA GATTCACGTC TTGGAGATGA TTATAACATC
    CCTCACTGGA TTAACCATAG CTTCTACACA TCGCAGAGCC AGCCTCTAAA TAACAGCTTT
    ACCCCACGCA TCAAACTGGC ACCTCGCAAG ACAAATAGTG AGAAAGGAAG GACTGGAAAA
    GACCCATCCA TAGGAAGCCC CAAAGAGACA GAGAATGCTC TTCCCATACA AGTAGACTAC
    GATGCTTATG ACTCACAGGT CTTCCGATTA CCAGGTCCAT CCAGGGCACA GCGAAGCATA
    ACTTTCAGGG CAGTGCGAGA GACACAAGTT CGAAAAAGTT CTTGTTCCTA TGACACATCC
    TGTAGCCCAG TCAGTCGGCC ATTTACAGCA GAGGAAGGCC GTAGCCAAAA TTCAGATGAG
    AGCTCTTTGG GAAAACTCTC CAACATCCTA CTTATCCCAA GGCCACATCT GCATCAATAT
    GAGGCCAGCA GCTCTTTGGG TTATACTAGC ACAAGAGATT TCTTGGAGAG GATGATTGAA
    TCCCAACAGC GAGACTCAAG TGCTCCTGGC AGATTCCATG TGGGCAGTGC AGAATCCATG
    TTACACGTCC GACCTGGGGG ATACCACCCG CAGAGAGCAC TCATCAACCC ATTTGCACCT
    TCACGTATGC CAATGAAACT GACATCAAAT CGCAGGAGGT GGATGCACAC ATTTCCAGTG
    GGCCCATCTG GAGAAGCAAT ACAAATACAT CACCAAACTC GTCAGAATAT GGCAGAGCTA
    CAGGGTAGTG GCATGCGTGA GGCATCTCAT TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC
    CATGAAGCCA CTGGCAGACA CAGTGGCCCT CGCACCCCTG GGGAAAATGG CTCTTGTGGG
    AGCAATGAAG AATTCTCCTG CAACACACCC ATCAATGGGC AGAGGAACCA CAATACAGGG
    TCTAATCACA GGGAGAAAGA GTCCTCTGAT GACAGCGTAC CATGCTGCCC AGAACCCATT
    TTGATGCTGT CTGCTCCCCC CGTAGTGCCT GGTTTCTGCT GCACGGTTGG GGTGGATTGG
    AAGTCCCTCA CCACCCCAGC CTGCCTGCCG CTTACCACAG ACTACTTCCC AGATAGGCAA
    AGTCTACAAA ATGATTACAC AGAGGGATGC TATGACCTTC TGCCAGAAGC TGACCTTGAC
    AGACGAGATG AAGAGGGAGC TCAGATGACT GCACAACAAG TTTTTGAAGA GTTTATCTGC
    CAACGTCTTA TGCAAGGTTA TCAAATTATT GTTCAGCCAA AACACCCAAA AGCCATCTCC
    TCTGTTCCTC CTCCACTCAG CAGCAGCCCG CTTTACAGAA GAGGGCTGGT GTCAAGGAAC
    AGAGCTGAAG AGGAGAACCA GTACTGGCTG AGTATGGGCC GAACCTTCCA TAAGGTGACA
    CTAAAGGACA AGATAATAAC AGTCACTAGA TATCTACCCA AGTATCCATA TGAATCTGCC
    CAAATCAGCT ACATGTACAG TCTGTGCCCA TCACACTCTG AGTGTGATTT TGTGTCCAGC
    TGGGTGGAAT TCTCCCATGA GCGACTGGAG GATTATAAAT GGAACTATTT GGATCAGTAT
    ATATGCTCAG CTGGCTCTGA AGATTTCAGT CTCATCGAAT CCCTAAAGTT TTGGCGCACA
    CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA CCCACAAAAC GCATCACTGA GGGGGAACAA
    CGCTGTGATA TATATGGTGA ACGCCCTCGA TCAGAACAAG AGGAAGCACA GTTGTTAGAC
    GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC CGAATCCGTC GACGCCACCG TTCTGATCGC
    ATGATCCGGA AAGGAGCAGG TATGAAGGGC CTGCAATTGC CTTCTCATCC GCCAGAACCT
    CCAGGTCCAC CTATAGGGAA GAAAGGAACA TCAGCACTAT CAGCATTGCT TGAGATGGAA
    GCTAATCAAA AGAGCCTGGG GGAGCAGCAA GCCCTTGTAC ATGCTGCAAA ATTGTCATCT
    CAGGTCTCAG ACCCTGCGGT AACTCCCACT TATGTGGACA GTCCTCGTAA GGATGGGGCC
    TTCTTTGTGG AGTTTGTACG CAGTCCACGC ACAGCGCCTT CCTTCACTCC TCAGATCTCC
    ATTGACCAAG GAACTTCTTT AGTGACTGAT GGAAATAATG CCATGCCTAA CCCTACCCCT
    TCTGAAAGAA TACAGAGTCA GGGTGCAACC TCTGTAGATC AGCCCCCCAC TTCAACTGAA
    TGCAGTGCAC AGCAGATATC TGTGGGTCCA AGTGTATTAC CAACCACTGC CAGTCTGAGT
    GAGATTCTGG AGGCTATGAA ACATCCAACT ACAGGCATCC CTCTCCTGTC AGAGCAGAAG
    TGCCTTCCTC CTGGTTGCTT TATCAGTGCT GAGGCTGTGC ATTGGCTGGT CAATAATATT
    AGCTGGGTGC AAAGTCAGGT TATGGCCATG GATATCATGC AGAAGATGCT TGAAGAGCAG
    TATATCACAC ATGCTTCAGG AGAGACATTT AGAACTTTCA TCTATGGCTT TTACTTGTAC
    AAGATCCCAG CTGACAAGGA GCCTGACAGA GCATCAAACC TGGTACAGCC ATGCGTGTGG
    CATTCCACAG CCATGGAGGA TTTCACAGCC TTCCAACGGA AGTGGTTTGA GGTTGCATTG
    GTGGAGGAAG AGCTACTGCA CTCCGAGATC CCACCTTTTC TTTTGCCGTG GTTACCTAGT
    CGCCCCGCAT CTTATGCAAG TAGGCACAGC TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC
    CAGGCCGCTG CTCTGCTAGC TGCTACAGTG CCAGAGCAGA GAACGGTCAC TTTAGATGTG
    GATGTTAATA ACCGCACTGA CCGGCTAGAA TGGTGCAGCA GCTACTATCA CGGAAACTTC
    TCCTTGACTG CTGCTTTTGA GATCAAACTG CACTGGATGG CAGCAACTGC AGCAATGCTA
    TTTGAGATGG TTCAAGGATG GCACAGAAAA GCTCCGTCCT GCGGTTTCCT CCTAGTACCT
    GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT TACTTGTATG GGGATCCTTT GCGTGCTCAG
    CTTTTTATTC CACTTAATAT CAGCTGCTTA GTAAAAGCTG AGAATGACCA TCTTTTTGAA
    GGTTTTGAAC CAGAAACTTA CTGGGAACGA ATGTACTTGC TACAGGAAGT AATAGCTTAT
    AGATTTGGCT TTATTCAGGA CAAATATTCA GCATCTGCAT TTAACTTCCC ATCAGAGAAT
    AAACCACAAT ATATACATGT GACAGGTACT GTATTCTTAC AGCTGCCTTA CTCAAAGCGG
    AAAGGACAGC AGAGACGTAG GAGGAATTCG ACCAGCTCTT CTACTCAGAA CCTTTTTGGA
    GAAGAACGCA TAGGGTATAA TTGGGCCTAC AATACCATGC TGACTAAAAC GTGGCGCTCC
    ATTGGTACAG GAGATGAGAG GTTTGCTGAC CGACTGCTTA AAGACTTTAC TGACTTCTGT
    GCCAACAGAG ATAACAGGTT AGGCACATTC TGGGCAAACT GCTTGGAGAA AATGAACACA
    AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748025.1
    CDS340..5091
    Translation

    Target ORF information:

    RefSeq Version XM_031892165.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892165.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGGACCA ACAGAGTATA CAAGCTAGTC ATCCACAAGA AAGGCTTTGG GGGCAGTGAT 
    GATGAGTTGG TGATAAACCC CAAGGTCTTT CCATATATTA ACCTGGGGGA CATTGTTGAA
    ATTGCTCACC CAAATGATGA ATACAGTCCC CTTCTCCTGC AGGTGAAGTC ACTAAAAGAA
    GATTTGCAAA AAGAAACTGT TAGTATTGAC CAAACCGTAG CCCAAGTGTT TCGCCTACGG
    CCATACCAGG ATGTTTATGT CAATGTTGTT GACCCTAAGG ATGTGACTCT TGATCTTGTG
    GAGTTAACAT TTAAGGACCA GTATATAGGT CGTGGAGATA TGTGGAGGCT AAAAAAGAGT
    TTGGTCAGCT CCTGTGCTTA CATAACTCAG AAAGTGGAGT TTGCTGGAAT TCGAGCACAA
    GTTGGAGAAT TATGGGTTAA GAGCGAGAAA GTCACCTGTG GGTACATCAG TGAAGACACA
    CGAGTTGTAT TCAGGTCCAC ATCGGCCATG GTGTATATAT TTATACAAAT GAGCAGTGAA
    CTGTGGGACT TTGATATCTA TGGTGATCTG TATTTTGAGA AGGCAGTAAA TGGATTTCTG
    GCTGATCTCT TCAACAAATG GAAGGACAAG AACTGCAGTC ACGAGGTTAC AGTGGTTCTA
    TTCTCTAGGA CCTTTTATGA TGCAAAGAAT ATTGAGGACT TTCCAGAAGC ACACCGTAAT
    GCGTCCGTCA TGCAAGATCA CAAGGGCAGA TTCTATGAAG ACTTCTACAA GGTGGTTGTA
    CAAAATGAAA GGCGAGAAGA ATGGACTTCA TTGCTAGTCA CTATTAAGAA ACTTTTTATT
    CAGTACCCAG TGCTTGTACG ACTGGAAAAT GCAGAGGGCT TTCCCCCAGG GCATAATTCT
    ACTTCAGATC AAGGAAATTA TCTAGAGGCC ATTAACCTGT CCTTTAATGT GTTTGACAAG
    CATTATATAA ATCGCAACTT TGACCGAACT GGTCAGATGT CTGTTGTGAT CACTCCAGGT
    GTTGGTGTTT TCCAAGTGGA TCGTTCACTA ATGATTCTGA CCAAGCAGCG AATGATTGAT
    AATGGGATTG GTGTGGATCT GGTATGTATG GGAGAGCAGC CGCTTCATGC TGTTCCACTT
    TTCAAGCTGC ACAATCGTTC CAGTCCAGGA GATTCACGTC TTGGAGATGA TTATAACATC
    CCTCACTGGA TTAACCATAG CTTCTACACA TCGCAGAGCC AGCCTCTAAA TAACAGCTTT
    ACCCCACGCA TCAAACTGGC ACCTCGCAAG ACAAATAGTG AGAAAGGAAG GACTGGAAAA
    GACCCATCCA TAGGAAGCCC CAAAGAGACA GAGAATGCTC TTCCCATACA AGTAGACTAC
    GATGCTTATG ACTCACAGGT CTTCCGATTA CCAGGTCCAT CCAGGGCACA GCGAAGCATA
    ACTTTCAGGG CAGTGCGAGA GACACAAGTT CGAAAAAGTT CTTGTTCCTA TGACACATCC
    TGTAGCCCAG TCAGTCGGCC ATTTACAGCA GAGGAAGGCC GTAGCCAAAA TTCAGATGAG
    AGCTCTTTGG GAAAACTCTC CAACATCCTA CTTATCCCAA GGCCACATCT GCATCAATAT
    GAGGCCAGCA GCTCTTTGGG TTATACTAGC ACAAGAGATT TCTTGGAGAG GATGATTGAA
    TCCCAACAGC GAGACTCAAG TGCTCCTGGC AGATTCCATG TGGGCAGTGC AGAATCCATG
    TTACACGTCC GACCTGGGGG ATACCACCCG CAGAGAGCAC TCATCAACCC ATTTGCACCT
    TCACGTATGC CAATGAAACT GACATCAAAT CGCAGGAGGT GGATGCACAC ATTTCCAGTG
    GGCCCATCTG GAGAAGCAAT ACAAATACAT CACCAAACTC GTCAGAATAT GGCAGAGCTA
    CAGGGTAGTG GCATGCGTGA GGCATCTCAT TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC
    CATGAAGCCA CTGGCAGACA CAGTGGCCCT CGCACCCCTG GGGAAAATGG CTCTTGTGGG
    AGCAATGAAG AATTCTCCTG CAACACACCC ATCAATGGGC AGAGGAACCA CAATACAGGG
    TCTAATCACA GGGAGAAAGA GTCCTCTGAT GACAGCGTAC CATGCTGCCC AGAACCCATT
    TTGATGCTGT CTGCTCCCCC CGTAGTGCCT GGTTTCTGCT GCACGGTTGG GGTGGATTGG
    AAGTCCCTCA CCACCCCAGC CTGCCTGCCG CTTACCACAG ACTACTTCCC AGATAGGCAA
    AGTCTACAAA ATGATTACAC AGAGGGATGC TATGACCTTC TGCCAGAAGC TGACCTTGAC
    AGACGAGATG AAGAGGGAGC TCAGATGACT GCACAACAAG TTTTTGAAGA GTTTATCTGC
    CAACGTCTTA TGCAAGGTTA TCAAATTATT GTTCAGCCAA AACACCCAAA AGCCATCTCC
    TCTGTTCCTC CTCCACTCAG CAGCAGCCCG CTTTACAGAA GAGGGCTGGT GTCAAGGAAC
    AGAGCTGAAG AGGAGAACCA GTACTGGCTG AGTATGGGCC GAACCTTCCA TAAGGTGACA
    CTAAAGGACA AGATAATAAC AGTCACTAGA TATCTACCCA AGTATCCATA TGAATCTGCC
    CAAATCAGCT ACATGTACAG TCTGTGCCCA TCACACTCTG AGTGTGATTT TGTGTCCAGC
    TGGGTGGAAT TCTCCCATGA GCGACTGGAG GATTATAAAT GGAACTATTT GGATCAGTAT
    ATATGCTCAG CTGGCTCTGA AGATTTCAGT CTCATCGAAT CCCTAAAGTT TTGGCGCACA
    CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA CCCACAAAAC GCATCACTGA GGGGGAACAA
    CGCTGTGATA TATATGGTGA ACGCCCTCGA TCAGAACAAG AGGAAGCACA GTTGTTAGAC
    GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC CGAATCCGTC GACGCCACCG TTCTGATCGC
    ATGATCCGGA AAGGAGCAGG TATGAAGGGC CTGCAATTGC CTTCTCATCC GCCAGAACCT
    CCAGGTCCAC CTATAGGGAA GAAAGGAACA TCAGCACTAT CAGCATTGCT TGAGATGGAA
    GCTAATCAAA AGAGCCTGGG GGAGCAGCAA GCCCTTGTAC ATGCTGCAAA ATTGTCATCT
    CAGGTCTCAG ACCCTGCGGT AACTCCCACT TATGTGGACA GTCCTCGTAA GGATGGGGCC
    TTCTTTGTGG AGTTTGTACG CAGTCCACGC ACAGCGCCTT CCTTCACTCC TCAGATCTCC
    ATTGACCAAG GAACTTCTTT AGTGACTGAT GGAAATAATG CCATGCCTAA CCCTACCCCT
    TCTGAAAGAA TACAGAGTCA GGGTGCAACC TCTGTAGATC AGCCCCCCAC TTCAACTGAA
    TGCAGTGCAC AGCAGATATC TGTGGGTCCA AGTGTATTAC CAACCACTGC CAGTCTGAGT
    GAGATTCTGG AGGCTATGAA ACATCCAACT ACAGGCATCC CTCTCCTGTC AGAGCAGAAG
    TGCCTTCCTC CTGGTTGCTT TATCAGTGCT GAGGCTGTGC ATTGGCTGGT CAATAATATT
    AGCTGGGTGC AAAGTCAGGT TATGGCCATG GATATCATGC AGAAGATGCT TGAAGAGCAG
    TATATCACAC ATGCTTCAGG AGAGACATTT AGAACTTTCA TCTATGGCTT TTACTTGTAC
    AAGATCCCAG CTGACAAGGA GCCTGACAGA GCATCAAACC TGGTACAGCC ATGCGTGTGG
    CATTCCACAG CCATGGAGGA TTTCACAGCC TTCCAACGGA AGTGGTTTGA GGTTGCATTG
    GTGGAGGAAG AGCTACTGCA CTCCGAGATC CCACCTTTTC TTTTGCCGTG GTTACCTAGT
    CGCCCCGCAT CTTATGCAAG TAGGCACAGC TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC
    CAGGCCGCTG CTCTGCTAGC TGCTACAGTG CCAGAGCAGA GAACGGTCAC TTTAGATGTG
    GATGTTAATA ACCGCACTGA CCGGCTAGAA TGGTGCAGCA GCTACTATCA CGGAAACTTC
    TCCTTGACTG CTGCTTTTGA GATCAAACTG CACTGGATGG CAGCAACTGC AGCAATGCTA
    TTTGAGATGG TTCAAGGATG GCACAGAAAA GCTCCGTCCT GCGGTTTCCT CCTAGTACCT
    GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT TACTTGTATG GGGATCCTTT GCGTGCTCAG
    CTTTTTATTC CACTTAATAT CAGCTGCTTA GTAAAAGCTG AGAATGACCA TCTTTTTGAA
    GGTTTTGAAC CAGAAACTTA CTGGGAACGA ATGTACTTGC TACAGGAAGT AATAGCTTAT
    AGATTTGGCT TTATTCAGGA CAAATATTCA GCATCTGCAT TTAACTTCCC ATCAGAGAAT
    AAACCACAAT ATATACATGT GACAGGTACT GTATTCTTAC AGCTGCCTTA CTCAAAGCGG
    AAAGGACAGC AGAGACGTAG GAGGAATTCG ACCAGCTCTT CTACTCAGAA CCTTTTTGGA
    GAAGAACGCA TAGGGTATAA TTGGGCCTAC AATACCATGC TGACTAAAAC GTGGCGCTCC
    ATTGGTACAG GAGATGAGAG GTTTGCTGAC CGACTGCTTA AAGACTTTAC TGACTTCTGT
    GCCAACAGAG ATAACAGGTT AGGCACATTC TGGGCAAACT GCTTGGAGAA AATGAACACA
    AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48888
    Clone ID Related Accession (Same CDS sequence) XM_031892165.1 , XM_031892148.1
    Accession Version XM_031892148.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4752bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGGACCA ACAGAGTATA CAAGCTAGTC ATCCACAAGA AAGGCTTTGG GGGCAGTGAT 
    GATGAGTTGG TGATAAACCC CAAGGTCTTT CCATATATTA ACCTGGGGGA CATTGTTGAA
    ATTGCTCACC CAAATGATGA ATACAGTCCC CTTCTCCTGC AGGTGAAGTC ACTAAAAGAA
    GATTTGCAAA AAGAAACTGT TAGTATTGAC CAAACCGTAG CCCAAGTGTT TCGCCTACGG
    CCATACCAGG ATGTTTATGT CAATGTTGTT GACCCTAAGG ATGTGACTCT TGATCTTGTG
    GAGTTAACAT TTAAGGACCA GTATATAGGT CGTGGAGATA TGTGGAGGCT AAAAAAGAGT
    TTGGTCAGCT CCTGTGCTTA CATAACTCAG AAAGTGGAGT TTGCTGGAAT TCGAGCACAA
    GTTGGAGAAT TATGGGTTAA GAGCGAGAAA GTCACCTGTG GGTACATCAG TGAAGACACA
    CGAGTTGTAT TCAGGTCCAC ATCGGCCATG GTGTATATAT TTATACAAAT GAGCAGTGAA
    CTGTGGGACT TTGATATCTA TGGTGATCTG TATTTTGAGA AGGCAGTAAA TGGATTTCTG
    GCTGATCTCT TCAACAAATG GAAGGACAAG AACTGCAGTC ACGAGGTTAC AGTGGTTCTA
    TTCTCTAGGA CCTTTTATGA TGCAAAGAAT ATTGAGGACT TTCCAGAAGC ACACCGTAAT
    GCGTCCGTCA TGCAAGATCA CAAGGGCAGA TTCTATGAAG ACTTCTACAA GGTGGTTGTA
    CAAAATGAAA GGCGAGAAGA ATGGACTTCA TTGCTAGTCA CTATTAAGAA ACTTTTTATT
    CAGTACCCAG TGCTTGTACG ACTGGAAAAT GCAGAGGGCT TTCCCCCAGG GCATAATTCT
    ACTTCAGATC AAGGAAATTA TCTAGAGGCC ATTAACCTGT CCTTTAATGT GTTTGACAAG
    CATTATATAA ATCGCAACTT TGACCGAACT GGTCAGATGT CTGTTGTGAT CACTCCAGGT
    GTTGGTGTTT TCCAAGTGGA TCGTTCACTA ATGATTCTGA CCAAGCAGCG AATGATTGAT
    AATGGGATTG GTGTGGATCT GGTATGTATG GGAGAGCAGC CGCTTCATGC TGTTCCACTT
    TTCAAGCTGC ACAATCGTTC CAGTCCAGGA GATTCACGTC TTGGAGATGA TTATAACATC
    CCTCACTGGA TTAACCATAG CTTCTACACA TCGCAGAGCC AGCCTCTAAA TAACAGCTTT
    ACCCCACGCA TCAAACTGGC ACCTCGCAAG ACAAATAGTG AGAAAGGAAG GACTGGAAAA
    GACCCATCCA TAGGAAGCCC CAAAGAGACA GAGAATGCTC TTCCCATACA AGTAGACTAC
    GATGCTTATG ACTCACAGGT CTTCCGATTA CCAGGTCCAT CCAGGGCACA GCGAAGCATA
    ACTTTCAGGG CAGTGCGAGA GACACAAGTT CGAAAAAGTT CTTGTTCCTA TGACACATCC
    TGTAGCCCAG TCAGTCGGCC ATTTACAGCA GAGGAAGGCC GTAGCCAAAA TTCAGATGAG
    AGCTCTTTGG GAAAACTCTC CAACATCCTA CTTATCCCAA GGCCACATCT GCATCAATAT
    GAGGCCAGCA GCTCTTTGGG TTATACTAGC ACAAGAGATT TCTTGGAGAG GATGATTGAA
    TCCCAACAGC GAGACTCAAG TGCTCCTGGC AGATTCCATG TGGGCAGTGC AGAATCCATG
    TTACACGTCC GACCTGGGGG ATACCACCCG CAGAGAGCAC TCATCAACCC ATTTGCACCT
    TCACGTATGC CAATGAAACT GACATCAAAT CGCAGGAGGT GGATGCACAC ATTTCCAGTG
    GGCCCATCTG GAGAAGCAAT ACAAATACAT CACCAAACTC GTCAGAATAT GGCAGAGCTA
    CAGGGTAGTG GCATGCGTGA GGCATCTCAT TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC
    CATGAAGCCA CTGGCAGACA CAGTGGCCCT CGCACCCCTG GGGAAAATGG CTCTTGTGGG
    AGCAATGAAG AATTCTCCTG CAACACACCC ATCAATGGGC AGAGGAACCA CAATACAGGG
    TCTAATCACA GGGAGAAAGA GTCCTCTGAT GACAGCGTAC CATGCTGCCC AGAACCCATT
    TTGATGCTGT CTGCTCCCCC CGTAGTGCCT GGTTTCTGCT GCACGGTTGG GGTGGATTGG
    AAGTCCCTCA CCACCCCAGC CTGCCTGCCG CTTACCACAG ACTACTTCCC AGATAGGCAA
    AGTCTACAAA ATGATTACAC AGAGGGATGC TATGACCTTC TGCCAGAAGC TGACCTTGAC
    AGACGAGATG AAGAGGGAGC TCAGATGACT GCACAACAAG TTTTTGAAGA GTTTATCTGC
    CAACGTCTTA TGCAAGGTTA TCAAATTATT GTTCAGCCAA AACACCCAAA AGCCATCTCC
    TCTGTTCCTC CTCCACTCAG CAGCAGCCCG CTTTACAGAA GAGGGCTGGT GTCAAGGAAC
    AGAGCTGAAG AGGAGAACCA GTACTGGCTG AGTATGGGCC GAACCTTCCA TAAGGTGACA
    CTAAAGGACA AGATAATAAC AGTCACTAGA TATCTACCCA AGTATCCATA TGAATCTGCC
    CAAATCAGCT ACATGTACAG TCTGTGCCCA TCACACTCTG AGTGTGATTT TGTGTCCAGC
    TGGGTGGAAT TCTCCCATGA GCGACTGGAG GATTATAAAT GGAACTATTT GGATCAGTAT
    ATATGCTCAG CTGGCTCTGA AGATTTCAGT CTCATCGAAT CCCTAAAGTT TTGGCGCACA
    CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA CCCACAAAAC GCATCACTGA GGGGGAACAA
    CGCTGTGATA TATATGGTGA ACGCCCTCGA TCAGAACAAG AGGAAGCACA GTTGTTAGAC
    GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC CGAATCCGTC GACGCCACCG TTCTGATCGC
    ATGATCCGGA AAGGAGCAGG TATGAAGGGC CTGCAATTGC CTTCTCATCC GCCAGAACCT
    CCAGGTCCAC CTATAGGGAA GAAAGGAACA TCAGCACTAT CAGCATTGCT TGAGATGGAA
    GCTAATCAAA AGAGCCTGGG GGAGCAGCAA GCCCTTGTAC ATGCTGCAAA ATTGTCATCT
    CAGGTCTCAG ACCCTGCGGT AACTCCCACT TATGTGGACA GTCCTCGTAA GGATGGGGCC
    TTCTTTGTGG AGTTTGTACG CAGTCCACGC ACAGCGCCTT CCTTCACTCC TCAGATCTCC
    ATTGACCAAG GAACTTCTTT AGTGACTGAT GGAAATAATG CCATGCCTAA CCCTACCCCT
    TCTGAAAGAA TACAGAGTCA GGGTGCAACC TCTGTAGATC AGCCCCCCAC TTCAACTGAA
    TGCAGTGCAC AGCAGATATC TGTGGGTCCA AGTGTATTAC CAACCACTGC CAGTCTGAGT
    GAGATTCTGG AGGCTATGAA ACATCCAACT ACAGGCATCC CTCTCCTGTC AGAGCAGAAG
    TGCCTTCCTC CTGGTTGCTT TATCAGTGCT GAGGCTGTGC ATTGGCTGGT CAATAATATT
    AGCTGGGTGC AAAGTCAGGT TATGGCCATG GATATCATGC AGAAGATGCT TGAAGAGCAG
    TATATCACAC ATGCTTCAGG AGAGACATTT AGAACTTTCA TCTATGGCTT TTACTTGTAC
    AAGATCCCAG CTGACAAGGA GCCTGACAGA GCATCAAACC TGGTACAGCC ATGCGTGTGG
    CATTCCACAG CCATGGAGGA TTTCACAGCC TTCCAACGGA AGTGGTTTGA GGTTGCATTG
    GTGGAGGAAG AGCTACTGCA CTCCGAGATC CCACCTTTTC TTTTGCCGTG GTTACCTAGT
    CGCCCCGCAT CTTATGCAAG TAGGCACAGC TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC
    CAGGCCGCTG CTCTGCTAGC TGCTACAGTG CCAGAGCAGA GAACGGTCAC TTTAGATGTG
    GATGTTAATA ACCGCACTGA CCGGCTAGAA TGGTGCAGCA GCTACTATCA CGGAAACTTC
    TCCTTGACTG CTGCTTTTGA GATCAAACTG CACTGGATGG CAGCAACTGC AGCAATGCTA
    TTTGAGATGG TTCAAGGATG GCACAGAAAA GCTCCGTCCT GCGGTTTCCT CCTAGTACCT
    GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT TACTTGTATG GGGATCCTTT GCGTGCTCAG
    CTTTTTATTC CACTTAATAT CAGCTGCTTA GTAAAAGCTG AGAATGACCA TCTTTTTGAA
    GGTTTTGAAC CAGAAACTTA CTGGGAACGA ATGTACTTGC TACAGGAAGT AATAGCTTAT
    AGATTTGGCT TTATTCAGGA CAAATATTCA GCATCTGCAT TTAACTTCCC ATCAGAGAAT
    AAACCACAAT ATATACATGT GACAGGTACT GTATTCTTAC AGCTGCCTTA CTCAAAGCGG
    AAAGGACAGC AGAGACGTAG GAGGAATTCG ACCAGCTCTT CTACTCAGAA CCTTTTTGGA
    GAAGAACGCA TAGGGTATAA TTGGGCCTAC AATACCATGC TGACTAAAAC GTGGCGCTCC
    ATTGGTACAG GAGATGAGAG GTTTGCTGAC CGACTGCTTA AAGACTTTAC TGACTTCTGT
    GCCAACAGAG ATAACAGGTT AGGCACATTC TGGGCAAACT GCTTGGAGAA AATGAACACA
    AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748008.1
    CDS55..4806
    Translation

    Target ORF information:

    RefSeq Version XM_031892148.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGGACCA ACAGAGTATA CAAGCTAGTC ATCCACAAGA AAGGCTTTGG GGGCAGTGAT 
    GATGAGTTGG TGATAAACCC CAAGGTCTTT CCATATATTA ACCTGGGGGA CATTGTTGAA
    ATTGCTCACC CAAATGATGA ATACAGTCCC CTTCTCCTGC AGGTGAAGTC ACTAAAAGAA
    GATTTGCAAA AAGAAACTGT TAGTATTGAC CAAACCGTAG CCCAAGTGTT TCGCCTACGG
    CCATACCAGG ATGTTTATGT CAATGTTGTT GACCCTAAGG ATGTGACTCT TGATCTTGTG
    GAGTTAACAT TTAAGGACCA GTATATAGGT CGTGGAGATA TGTGGAGGCT AAAAAAGAGT
    TTGGTCAGCT CCTGTGCTTA CATAACTCAG AAAGTGGAGT TTGCTGGAAT TCGAGCACAA
    GTTGGAGAAT TATGGGTTAA GAGCGAGAAA GTCACCTGTG GGTACATCAG TGAAGACACA
    CGAGTTGTAT TCAGGTCCAC ATCGGCCATG GTGTATATAT TTATACAAAT GAGCAGTGAA
    CTGTGGGACT TTGATATCTA TGGTGATCTG TATTTTGAGA AGGCAGTAAA TGGATTTCTG
    GCTGATCTCT TCAACAAATG GAAGGACAAG AACTGCAGTC ACGAGGTTAC AGTGGTTCTA
    TTCTCTAGGA CCTTTTATGA TGCAAAGAAT ATTGAGGACT TTCCAGAAGC ACACCGTAAT
    GCGTCCGTCA TGCAAGATCA CAAGGGCAGA TTCTATGAAG ACTTCTACAA GGTGGTTGTA
    CAAAATGAAA GGCGAGAAGA ATGGACTTCA TTGCTAGTCA CTATTAAGAA ACTTTTTATT
    CAGTACCCAG TGCTTGTACG ACTGGAAAAT GCAGAGGGCT TTCCCCCAGG GCATAATTCT
    ACTTCAGATC AAGGAAATTA TCTAGAGGCC ATTAACCTGT CCTTTAATGT GTTTGACAAG
    CATTATATAA ATCGCAACTT TGACCGAACT GGTCAGATGT CTGTTGTGAT CACTCCAGGT
    GTTGGTGTTT TCCAAGTGGA TCGTTCACTA ATGATTCTGA CCAAGCAGCG AATGATTGAT
    AATGGGATTG GTGTGGATCT GGTATGTATG GGAGAGCAGC CGCTTCATGC TGTTCCACTT
    TTCAAGCTGC ACAATCGTTC CAGTCCAGGA GATTCACGTC TTGGAGATGA TTATAACATC
    CCTCACTGGA TTAACCATAG CTTCTACACA TCGCAGAGCC AGCCTCTAAA TAACAGCTTT
    ACCCCACGCA TCAAACTGGC ACCTCGCAAG ACAAATAGTG AGAAAGGAAG GACTGGAAAA
    GACCCATCCA TAGGAAGCCC CAAAGAGACA GAGAATGCTC TTCCCATACA AGTAGACTAC
    GATGCTTATG ACTCACAGGT CTTCCGATTA CCAGGTCCAT CCAGGGCACA GCGAAGCATA
    ACTTTCAGGG CAGTGCGAGA GACACAAGTT CGAAAAAGTT CTTGTTCCTA TGACACATCC
    TGTAGCCCAG TCAGTCGGCC ATTTACAGCA GAGGAAGGCC GTAGCCAAAA TTCAGATGAG
    AGCTCTTTGG GAAAACTCTC CAACATCCTA CTTATCCCAA GGCCACATCT GCATCAATAT
    GAGGCCAGCA GCTCTTTGGG TTATACTAGC ACAAGAGATT TCTTGGAGAG GATGATTGAA
    TCCCAACAGC GAGACTCAAG TGCTCCTGGC AGATTCCATG TGGGCAGTGC AGAATCCATG
    TTACACGTCC GACCTGGGGG ATACCACCCG CAGAGAGCAC TCATCAACCC ATTTGCACCT
    TCACGTATGC CAATGAAACT GACATCAAAT CGCAGGAGGT GGATGCACAC ATTTCCAGTG
    GGCCCATCTG GAGAAGCAAT ACAAATACAT CACCAAACTC GTCAGAATAT GGCAGAGCTA
    CAGGGTAGTG GCATGCGTGA GGCATCTCAT TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC
    CATGAAGCCA CTGGCAGACA CAGTGGCCCT CGCACCCCTG GGGAAAATGG CTCTTGTGGG
    AGCAATGAAG AATTCTCCTG CAACACACCC ATCAATGGGC AGAGGAACCA CAATACAGGG
    TCTAATCACA GGGAGAAAGA GTCCTCTGAT GACAGCGTAC CATGCTGCCC AGAACCCATT
    TTGATGCTGT CTGCTCCCCC CGTAGTGCCT GGTTTCTGCT GCACGGTTGG GGTGGATTGG
    AAGTCCCTCA CCACCCCAGC CTGCCTGCCG CTTACCACAG ACTACTTCCC AGATAGGCAA
    AGTCTACAAA ATGATTACAC AGAGGGATGC TATGACCTTC TGCCAGAAGC TGACCTTGAC
    AGACGAGATG AAGAGGGAGC TCAGATGACT GCACAACAAG TTTTTGAAGA GTTTATCTGC
    CAACGTCTTA TGCAAGGTTA TCAAATTATT GTTCAGCCAA AACACCCAAA AGCCATCTCC
    TCTGTTCCTC CTCCACTCAG CAGCAGCCCG CTTTACAGAA GAGGGCTGGT GTCAAGGAAC
    AGAGCTGAAG AGGAGAACCA GTACTGGCTG AGTATGGGCC GAACCTTCCA TAAGGTGACA
    CTAAAGGACA AGATAATAAC AGTCACTAGA TATCTACCCA AGTATCCATA TGAATCTGCC
    CAAATCAGCT ACATGTACAG TCTGTGCCCA TCACACTCTG AGTGTGATTT TGTGTCCAGC
    TGGGTGGAAT TCTCCCATGA GCGACTGGAG GATTATAAAT GGAACTATTT GGATCAGTAT
    ATATGCTCAG CTGGCTCTGA AGATTTCAGT CTCATCGAAT CCCTAAAGTT TTGGCGCACA
    CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA CCCACAAAAC GCATCACTGA GGGGGAACAA
    CGCTGTGATA TATATGGTGA ACGCCCTCGA TCAGAACAAG AGGAAGCACA GTTGTTAGAC
    GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC CGAATCCGTC GACGCCACCG TTCTGATCGC
    ATGATCCGGA AAGGAGCAGG TATGAAGGGC CTGCAATTGC CTTCTCATCC GCCAGAACCT
    CCAGGTCCAC CTATAGGGAA GAAAGGAACA TCAGCACTAT CAGCATTGCT TGAGATGGAA
    GCTAATCAAA AGAGCCTGGG GGAGCAGCAA GCCCTTGTAC ATGCTGCAAA ATTGTCATCT
    CAGGTCTCAG ACCCTGCGGT AACTCCCACT TATGTGGACA GTCCTCGTAA GGATGGGGCC
    TTCTTTGTGG AGTTTGTACG CAGTCCACGC ACAGCGCCTT CCTTCACTCC TCAGATCTCC
    ATTGACCAAG GAACTTCTTT AGTGACTGAT GGAAATAATG CCATGCCTAA CCCTACCCCT
    TCTGAAAGAA TACAGAGTCA GGGTGCAACC TCTGTAGATC AGCCCCCCAC TTCAACTGAA
    TGCAGTGCAC AGCAGATATC TGTGGGTCCA AGTGTATTAC CAACCACTGC CAGTCTGAGT
    GAGATTCTGG AGGCTATGAA ACATCCAACT ACAGGCATCC CTCTCCTGTC AGAGCAGAAG
    TGCCTTCCTC CTGGTTGCTT TATCAGTGCT GAGGCTGTGC ATTGGCTGGT CAATAATATT
    AGCTGGGTGC AAAGTCAGGT TATGGCCATG GATATCATGC AGAAGATGCT TGAAGAGCAG
    TATATCACAC ATGCTTCAGG AGAGACATTT AGAACTTTCA TCTATGGCTT TTACTTGTAC
    AAGATCCCAG CTGACAAGGA GCCTGACAGA GCATCAAACC TGGTACAGCC ATGCGTGTGG
    CATTCCACAG CCATGGAGGA TTTCACAGCC TTCCAACGGA AGTGGTTTGA GGTTGCATTG
    GTGGAGGAAG AGCTACTGCA CTCCGAGATC CCACCTTTTC TTTTGCCGTG GTTACCTAGT
    CGCCCCGCAT CTTATGCAAG TAGGCACAGC TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC
    CAGGCCGCTG CTCTGCTAGC TGCTACAGTG CCAGAGCAGA GAACGGTCAC TTTAGATGTG
    GATGTTAATA ACCGCACTGA CCGGCTAGAA TGGTGCAGCA GCTACTATCA CGGAAACTTC
    TCCTTGACTG CTGCTTTTGA GATCAAACTG CACTGGATGG CAGCAACTGC AGCAATGCTA
    TTTGAGATGG TTCAAGGATG GCACAGAAAA GCTCCGTCCT GCGGTTTCCT CCTAGTACCT
    GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT TACTTGTATG GGGATCCTTT GCGTGCTCAG
    CTTTTTATTC CACTTAATAT CAGCTGCTTA GTAAAAGCTG AGAATGACCA TCTTTTTGAA
    GGTTTTGAAC CAGAAACTTA CTGGGAACGA ATGTACTTGC TACAGGAAGT AATAGCTTAT
    AGATTTGGCT TTATTCAGGA CAAATATTCA GCATCTGCAT TTAACTTCCC ATCAGAGAAT
    AAACCACAAT ATATACATGT GACAGGTACT GTATTCTTAC AGCTGCCTTA CTCAAAGCGG
    AAAGGACAGC AGAGACGTAG GAGGAATTCG ACCAGCTCTT CTACTCAGAA CCTTTTTGGA
    GAAGAACGCA TAGGGTATAA TTGGGCCTAC AATACCATGC TGACTAAAAC GTGGCGCTCC
    ATTGGTACAG GAGATGAGAG GTTTGCTGAC CGACTGCTTA AAGACTTTAC TGACTTCTGT
    GCCAACAGAG ATAACAGGTT AGGCACATTC TGGGCAAACT GCTTGGAGAA AATGAACACA
    AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48889
    Clone ID Related Accession (Same CDS sequence) XM_002931918.5
    Accession Version XM_002931918.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_002931918.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATGCCTGG TTTCTGCTGC ACGGTTGGGG TGGATTGGAA GTCCCTCACC
    ACCCCAGCCT GCCTGCCGCT TACCACAGAC TACTTCCCAG ATAGGCAAAG TCTACAAAAT
    GATTACACAG AGGGATGCTA TGACCTTCTG CCAGAAGCTG ACCTTGACAG ACGAGATGAA
    GAGGGAGCTC AGATGACTGC ACAACAAGTT TTTGAAGAGT TTATCTGCCA ACGTCTTATG
    CAAGGTTATC AAATTATTGT TCAGCCAAAA CACCCAAAAG CCATCTCCTC TGTTCCTCCT
    CCACTCAGCA GCAGCCCGCT TTACAGAAGA GGGCTGGTGT CAAGGAACAG AGCTGAAGAG
    GAGAACCAGT ACTGGCTGAG TATGGGCCGA ACCTTCCATA AGGTGACACT AAAGGACAAG
    ATAATAACAG TCACTAGATA TCTACCCAAG TATCCATATG AATCTGCCCA AATCAGCTAC
    ATGTACAGTC TGTGCCCATC ACACTCTGAG TGTGATTTTG TGTCCAGCTG GGTGGAATTC
    TCCCATGAGC GACTGGAGGA TTATAAATGG AACTATTTGG ATCAGTATAT ATGCTCAGCT
    GGCTCTGAAG ATTTCAGTCT CATCGAATCC CTAAAGTTTT GGCGCACACG TTTCCTTTTG
    CTCCCTTCTT GTGTGGGACC CACAAAACGC ATCACTGAGG GGGAACAACG CTGTGATATA
    TATGGTGAAC GCCCTCGATC AGAACAAGAG GAAGCACAGT TGTTAGACGG CTTTTTACGC
    TTTGTGGAGG GGCTAAACCG AATCCGTCGA CGCCACCGTT CTGATCGCAT GATCCGGAAA
    GGAGCAGGTA TGAAGGGCCT GCAATTGCCT TCTCATCCGC CAGAACCTCC AGGTCCACCT
    ATAGGGAAGA AAGGAACATC AGCACTATCA GCATTGCTTG AGATGGAAGC TAATCAAAAG
    AGCCTGGGGG AGCAGCAAGC CCTTGTACAT GCTGCAAAAT TGTCATCTCA GGTCTCAGAC
    CCTGCGGTAA CTCCCACTTA TGTGGACAGT CCTCGTAAGG ATGGGGCCTT CTTTGTGGAG
    TTTGTACGCA GTCCACGCAC AGCGCCTTCC TTCACTCCTC AGATCTCCAT TGACCAAGGA
    ACTTCTTTAG TGACTGATGG AAATAATGCC ATGCCTAACC CTACCCCTTC TGAAAGAATA
    CAGAGTCAGG GTGCAACCTC TGTAGATCAG CCCCCCACTT CAACTGAATG CAGTGCACAG
    CAGATATCTG TGGGTCCAAG TGTATTACCA ACCACTGCCA GTCTGAGTGA GATTCTGGAG
    GCTATGAAAC ATCCAACTAC AGGCATCCCT CTCCTGTCAG AGCAGAAGTG CCTTCCTCCT
    GGTTGCTTTA TCAGTGCTGA GGCTGTGCAT TGGCTGGTCA ATAATATTAG CTGGGTGCAA
    AGTCAGGTTA TGGCCATGGA TATCATGCAG AAGATGCTTG AAGAGCAGTA TATCACACAT
    GCTTCAGGAG AGACATTTAG AACTTTCATC TATGGCTTTT ACTTGTACAA GATCCCAGCT
    GACAAGGAGC CTGACAGAGC ATCAAACCTG GTACAGCCAT GCGTGTGGCA TTCCACAGCC
    ATGGAGGATT TCACAGCCTT CCAACGGAAG TGGTTTGAGG TTGCATTGGT GGAGGAAGAG
    CTACTGCACT CCGAGATCCC ACCTTTTCTT TTGCCGTGGT TACCTAGTCG CCCCGCATCT
    TATGCAAGTA GGCACAGCTC CTTTAGCCGC AGCTTCGGAG GAAGGAGCCA GGCCGCTGCT
    CTGCTAGCTG CTACAGTGCC AGAGCAGAGA ACGGTCACTT TAGATGTGGA TGTTAATAAC
    CGCACTGACC GGCTAGAATG GTGCAGCAGC TACTATCACG GAAACTTCTC CTTGACTGCT
    GCTTTTGAGA TCAAACTGCA CTGGATGGCA GCAACTGCAG CAATGCTATT TGAGATGGTT
    CAAGGATGGC ACAGAAAAGC TCCGTCCTGC GGTTTCCTCC TAGTACCTGT TGTAGAAGGC
    CCTTTTTCTC TCCCTAGTTA CTTGTATGGG GATCCTTTGC GTGCTCAGCT TTTTATTCCA
    CTTAATATCA GCTGCTTAGT AAAAGCTGAG AATGACCATC TTTTTGAAGG TTTTGAACCA
    GAAACTTACT GGGAACGAAT GTACTTGCTA CAGGAAGTAA TAGCTTATAG ATTTGGCTTT
    ATTCAGGACA AATATTCAGC ATCTGCATTT AACTTCCCAT CAGAGAATAA ACCACAATAT
    ATACATGTGA CAGGTACTGT ATTCTTACAG CTGCCTTACT CAAAGCGGAA AGGACAGCAG
    AGACGTAGGA GGAATTCGAC CAGCTCTTCT ACTCAGAACC TTTTTGGAGA AGAACGCATA
    GGGTATAATT GGGCCTACAA TACCATGCTG ACTAAAACGT GGCGCTCCAT TGGTACAGGA
    GATGAGAGGT TTGCTGACCG ACTGCTTAAA GACTTTACTG ACTTCTGTGC CAACAGAGAT
    AACAGGTTAG GCACATTCTG GGCAAACTGC TTGGAGAAAA TGAACACAAG TGCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931964.2
    CDS306..5105
    Translation

    Target ORF information:

    RefSeq Version XM_002931918.5
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931918.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATGCCTGG TTTCTGCTGC ACGGTTGGGG TGGATTGGAA GTCCCTCACC
    ACCCCAGCCT GCCTGCCGCT TACCACAGAC TACTTCCCAG ATAGGCAAAG TCTACAAAAT
    GATTACACAG AGGGATGCTA TGACCTTCTG CCAGAAGCTG ACCTTGACAG ACGAGATGAA
    GAGGGAGCTC AGATGACTGC ACAACAAGTT TTTGAAGAGT TTATCTGCCA ACGTCTTATG
    CAAGGTTATC AAATTATTGT TCAGCCAAAA CACCCAAAAG CCATCTCCTC TGTTCCTCCT
    CCACTCAGCA GCAGCCCGCT TTACAGAAGA GGGCTGGTGT CAAGGAACAG AGCTGAAGAG
    GAGAACCAGT ACTGGCTGAG TATGGGCCGA ACCTTCCATA AGGTGACACT AAAGGACAAG
    ATAATAACAG TCACTAGATA TCTACCCAAG TATCCATATG AATCTGCCCA AATCAGCTAC
    ATGTACAGTC TGTGCCCATC ACACTCTGAG TGTGATTTTG TGTCCAGCTG GGTGGAATTC
    TCCCATGAGC GACTGGAGGA TTATAAATGG AACTATTTGG ATCAGTATAT ATGCTCAGCT
    GGCTCTGAAG ATTTCAGTCT CATCGAATCC CTAAAGTTTT GGCGCACACG TTTCCTTTTG
    CTCCCTTCTT GTGTGGGACC CACAAAACGC ATCACTGAGG GGGAACAACG CTGTGATATA
    TATGGTGAAC GCCCTCGATC AGAACAAGAG GAAGCACAGT TGTTAGACGG CTTTTTACGC
    TTTGTGGAGG GGCTAAACCG AATCCGTCGA CGCCACCGTT CTGATCGCAT GATCCGGAAA
    GGAGCAGGTA TGAAGGGCCT GCAATTGCCT TCTCATCCGC CAGAACCTCC AGGTCCACCT
    ATAGGGAAGA AAGGAACATC AGCACTATCA GCATTGCTTG AGATGGAAGC TAATCAAAAG
    AGCCTGGGGG AGCAGCAAGC CCTTGTACAT GCTGCAAAAT TGTCATCTCA GGTCTCAGAC
    CCTGCGGTAA CTCCCACTTA TGTGGACAGT CCTCGTAAGG ATGGGGCCTT CTTTGTGGAG
    TTTGTACGCA GTCCACGCAC AGCGCCTTCC TTCACTCCTC AGATCTCCAT TGACCAAGGA
    ACTTCTTTAG TGACTGATGG AAATAATGCC ATGCCTAACC CTACCCCTTC TGAAAGAATA
    CAGAGTCAGG GTGCAACCTC TGTAGATCAG CCCCCCACTT CAACTGAATG CAGTGCACAG
    CAGATATCTG TGGGTCCAAG TGTATTACCA ACCACTGCCA GTCTGAGTGA GATTCTGGAG
    GCTATGAAAC ATCCAACTAC AGGCATCCCT CTCCTGTCAG AGCAGAAGTG CCTTCCTCCT
    GGTTGCTTTA TCAGTGCTGA GGCTGTGCAT TGGCTGGTCA ATAATATTAG CTGGGTGCAA
    AGTCAGGTTA TGGCCATGGA TATCATGCAG AAGATGCTTG AAGAGCAGTA TATCACACAT
    GCTTCAGGAG AGACATTTAG AACTTTCATC TATGGCTTTT ACTTGTACAA GATCCCAGCT
    GACAAGGAGC CTGACAGAGC ATCAAACCTG GTACAGCCAT GCGTGTGGCA TTCCACAGCC
    ATGGAGGATT TCACAGCCTT CCAACGGAAG TGGTTTGAGG TTGCATTGGT GGAGGAAGAG
    CTACTGCACT CCGAGATCCC ACCTTTTCTT TTGCCGTGGT TACCTAGTCG CCCCGCATCT
    TATGCAAGTA GGCACAGCTC CTTTAGCCGC AGCTTCGGAG GAAGGAGCCA GGCCGCTGCT
    CTGCTAGCTG CTACAGTGCC AGAGCAGAGA ACGGTCACTT TAGATGTGGA TGTTAATAAC
    CGCACTGACC GGCTAGAATG GTGCAGCAGC TACTATCACG GAAACTTCTC CTTGACTGCT
    GCTTTTGAGA TCAAACTGCA CTGGATGGCA GCAACTGCAG CAATGCTATT TGAGATGGTT
    CAAGGATGGC ACAGAAAAGC TCCGTCCTGC GGTTTCCTCC TAGTACCTGT TGTAGAAGGC
    CCTTTTTCTC TCCCTAGTTA CTTGTATGGG GATCCTTTGC GTGCTCAGCT TTTTATTCCA
    CTTAATATCA GCTGCTTAGT AAAAGCTGAG AATGACCATC TTTTTGAAGG TTTTGAACCA
    GAAACTTACT GGGAACGAAT GTACTTGCTA CAGGAAGTAA TAGCTTATAG ATTTGGCTTT
    ATTCAGGACA AATATTCAGC ATCTGCATTT AACTTCCCAT CAGAGAATAA ACCACAATAT
    ATACATGTGA CAGGTACTGT ATTCTTACAG CTGCCTTACT CAAAGCGGAA AGGACAGCAG
    AGACGTAGGA GGAATTCGAC CAGCTCTTCT ACTCAGAACC TTTTTGGAGA AGAACGCATA
    GGGTATAATT GGGCCTACAA TACCATGCTG ACTAAAACGT GGCGCTCCAT TGGTACAGGA
    GATGAGAGGT TTGCTGACCG ACTGCTTAAA GACTTTACTG ACTTCTGTGC CAACAGAGAT
    AACAGGTTAG GCACATTCTG GGCAAACTGC TTGGAGAAAA TGAACACAAG TGCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48890
    Clone ID Related Accession (Same CDS sequence) XM_031892156.1
    Accession Version XM_031892156.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4767bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT ACAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCT TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAACTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG CAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAATATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748016.1
    CDS306..5072
    Translation

    Target ORF information:

    RefSeq Version XM_031892156.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892156.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT ACAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCT TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAACTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG CAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAATATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48891
    Clone ID Related Accession (Same CDS sequence) XM_031892162.1
    Accession Version XM_031892162.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4764bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT ACAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGATCT CCATTGACCA AGGAACTTCT TTAGTGACTG ATGGAAATAA TGCCATGCCT
    AACCCTACCC CTTCTGAAAG AATACAGAGT CAGGGTGCAA CCTCTGTAGA TCAGCCCCCC
    ACTTCAACTG AATGCAGTGC ACAGCAGATA TCTGTGGGTC CAAGTGTATT ACCAACCACT
    GCCAGTCTGA GTGAGATTCT GGAGGCTATG AAACATCCAA CTACAGGCAT CCCTCTCCTG
    TCAGAGCAGA AGTGCCTTCC TCCTGGTTGC TTTATCAGTG CTGAGGCTGT GCATTGGCTG
    GTCAATAATA TTAGCTGGGT GCAAAGTCAG GTTATGGCCA TGGATATCAT GCAGAAGATG
    CTTGAAGAGC AGTATATCAC ACATGCTTCA GGAGAGACAT TTAGAACTTT CATCTATGGC
    TTTTACTTGT ACAAGATCCC AGCTGACAAG GAGCCTGACA GAGCATCAAA CCTGGTACAG
    CCATGCGTGT GGCATTCCAC AGCCATGGAG GATTTCACAG CCTTCCAACG GAAGTGGTTT
    GAGGTTGCAT TGGTGGAGGA AGAGCTACTG CACTCCGAGA TCCCACCTTT TCTTTTGCCG
    TGGTTACCTA GTCGCCCCGC ATCTTATGCA AGTAGGCACA GCTCCTTTAG CCGCAGCTTC
    GGAGGAAGGA GCCAGGCCGC TGCTCTGCTA GCTGCTACAG TGCCAGAGCA GAGAACGGTC
    ACTTTAGATG TGGATGTTAA TAACCGCACT GACCGGCTAG AATGGTGCAG CAGCTACTAT
    CACGGAAACT TCTCCTTGAC TGCTGCTTTT GAGATCAAAC TGCACTGGAT GGCAGCAACT
    GCAGCAATGC TATTTGAGAT GGTTCAAGGA TGGCACAGAA AAGCTCCGTC CTGCGGTTTC
    CTCCTAGTAC CTGTTGTAGA AGGCCCTTTT TCTCTCCCTA GTTACTTGTA TGGGGATCCT
    TTGCGTGCTC AGCTTTTTAT TCCACTTAAT ATCAGCTGCT TAGTAAAAGC TGAGAATGAC
    CATCTTTTTG AAGGTTTTGA ACCAGAAACT TACTGGGAAC GAATGTACTT GCTACAGGAA
    GTAATAGCTT ATAGATTTGG CTTTATTCAG GACAAATATT CAGCATCTGC ATTTAACTTC
    CCATCAGAGA ATAAACCACA ATATATACAT GTGACAGGTA CTGTATTCTT ACAGCTGCCT
    TACTCAAAGC GGAAAGGACA GCAGAGACGT AGGAGGAATT CGACCAGCTC TTCTACTCAG
    AACCTTTTTG GAGAAGAACG CATAGGGTAT AATTGGGCCT ACAATACCAT GCTGACTAAA
    ACGTGGCGCT CCATTGGTAC AGGAGATGAG AGGTTTGCTG ACCGACTGCT TAAAGACTTT
    ACTGACTTCT GTGCCAACAG AGATAACAGG TTAGGCACAT TCTGGGCAAA CTGCTTGGAG
    AAAATGAACA CAAGTGCGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748022.1
    CDS306..5069
    Translation

    Target ORF information:

    RefSeq Version XM_031892162.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892162.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT ACAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGATCT CCATTGACCA AGGAACTTCT TTAGTGACTG ATGGAAATAA TGCCATGCCT
    AACCCTACCC CTTCTGAAAG AATACAGAGT CAGGGTGCAA CCTCTGTAGA TCAGCCCCCC
    ACTTCAACTG AATGCAGTGC ACAGCAGATA TCTGTGGGTC CAAGTGTATT ACCAACCACT
    GCCAGTCTGA GTGAGATTCT GGAGGCTATG AAACATCCAA CTACAGGCAT CCCTCTCCTG
    TCAGAGCAGA AGTGCCTTCC TCCTGGTTGC TTTATCAGTG CTGAGGCTGT GCATTGGCTG
    GTCAATAATA TTAGCTGGGT GCAAAGTCAG GTTATGGCCA TGGATATCAT GCAGAAGATG
    CTTGAAGAGC AGTATATCAC ACATGCTTCA GGAGAGACAT TTAGAACTTT CATCTATGGC
    TTTTACTTGT ACAAGATCCC AGCTGACAAG GAGCCTGACA GAGCATCAAA CCTGGTACAG
    CCATGCGTGT GGCATTCCAC AGCCATGGAG GATTTCACAG CCTTCCAACG GAAGTGGTTT
    GAGGTTGCAT TGGTGGAGGA AGAGCTACTG CACTCCGAGA TCCCACCTTT TCTTTTGCCG
    TGGTTACCTA GTCGCCCCGC ATCTTATGCA AGTAGGCACA GCTCCTTTAG CCGCAGCTTC
    GGAGGAAGGA GCCAGGCCGC TGCTCTGCTA GCTGCTACAG TGCCAGAGCA GAGAACGGTC
    ACTTTAGATG TGGATGTTAA TAACCGCACT GACCGGCTAG AATGGTGCAG CAGCTACTAT
    CACGGAAACT TCTCCTTGAC TGCTGCTTTT GAGATCAAAC TGCACTGGAT GGCAGCAACT
    GCAGCAATGC TATTTGAGAT GGTTCAAGGA TGGCACAGAA AAGCTCCGTC CTGCGGTTTC
    CTCCTAGTAC CTGTTGTAGA AGGCCCTTTT TCTCTCCCTA GTTACTTGTA TGGGGATCCT
    TTGCGTGCTC AGCTTTTTAT TCCACTTAAT ATCAGCTGCT TAGTAAAAGC TGAGAATGAC
    CATCTTTTTG AAGGTTTTGA ACCAGAAACT TACTGGGAAC GAATGTACTT GCTACAGGAA
    GTAATAGCTT ATAGATTTGG CTTTATTCAG GACAAATATT CAGCATCTGC ATTTAACTTC
    CCATCAGAGA ATAAACCACA ATATATACAT GTGACAGGTA CTGTATTCTT ACAGCTGCCT
    TACTCAAAGC GGAAAGGACA GCAGAGACGT AGGAGGAATT CGACCAGCTC TTCTACTCAG
    AACCTTTTTG GAGAAGAACG CATAGGGTAT AATTGGGCCT ACAATACCAT GCTGACTAAA
    ACGTGGCGCT CCATTGGTAC AGGAGATGAG AGGTTTGCTG ACCGACTGCT TAAAGACTTT
    ACTGACTTCT GTGCCAACAG AGATAACAGG TTAGGCACAT TCTGGGCAAA CTGCTTGGAG
    AAAATGAACA CAAGTGCGCC TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa48892
    Clone ID Related Accession (Same CDS sequence) XM_031892174.1
    Accession Version XM_031892174.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4740bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-17
    Organism Xenopus tropicalis(tropical clawed frog)
    Product GATOR complex protein DEPDC5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGTGCCTGG TTTCTGCTGC ACGGTTGGGG TGGATTGGAA GTCCCTCACC
    ACCCCAGCCT GCCTGCCGCT TACCACAGAC TACTTCCCAG ATAGGCAAAG TCTACAAAAT
    GATTACACAG AGGGATGCTA TGACCTTCTG CCAGAAGCTG ACCTTGACAG ACGAGATGAA
    GAGGGAGCTC AGATGACTGC ACAACAAGTT TTTGAAGAGT TTATCTGCCA ACGTCTTATG
    CAAGGTTATC AAATTATTGT TCAGCCAAAA CACCCAAAAG CCATCTCCTC TGTTCCTCCT
    CCACTCAGCA GCAGCCCGCT TTACAGAAGA GGGCTGGTGT CAAGGAACAG AGCTGAAGAG
    GAGAACCAGT ACTGGCTGAG TATGGGCCGA ACCTTCCATA AGGTGACACT AAAGGACAAG
    ATAATAACAG TCACTAGATA TCTACCCAAG TATCCATATG AATCTGCCCA AATCAGCTAC
    ATGTACAGTC TGTGCCCATC ACACTCTGAG TGTGATTTTG TGTCCAGCTG GGTGGAATTC
    TCCCATGAGC GACTGGAGGA TTATAAATGG AACTATTTGG ATCAGTATAT ATGCTCAGCT
    GGCTCTGAAG ATTTCAGTCT CATCGAATCC CTAAAGTTTT GGCGCACACG TTTCCTTTTG
    CTCCCTTCTT GTGTGGGACC CACAAAACGC ATCACTGAGG GGGAACAACG CTGTGATATA
    TATGGTGAAC GCCCTCGATC AGAACAAGAG GAAGCACAGT TGTTAGACGG CTTTTTACGC
    TTTGTGGAGG GGCTAAACCG AATCCGTCGA CGCCACCGTT CTGATCGCAT GATCCGGAAA
    GGAGCAGGTA TGAAGGGCCT GCAATTGCCT TCTCATCCGC CAGAACCTCC AGGTCCACCT
    ATAGGGAAGA AAGGAACATC AGCACTATCA GCATTGCTTG AGATGGAAGC TAATCAAAAG
    AGCCTGGGGG AGCAGCAAGC CCTTGTACAT GCTGCAAAAT TGTCATCTCA GGTCTCAGAC
    CCTGCGGTAA CTCCCACTTA TGTGGACAGT CCTCGTAAGG ATGGGGCCTT CTTTGTGGAG
    TTTGTACGCA GTCCACGCAC AGCGCCTTCC TTCACTCCTC AGATCTCCAT TGACCAAGGA
    ACTTCTTTAG TGACTGATGG AAATAATGCC ATGCCTAACC CTACCCCTTC TGAAAGAATA
    CAGAGTCAGG GTGCAACCTC TGTAGATCAG CCCCCCACTT CAACTGAATG CAGTGCACAG
    CAGATATCTG TGGGTCCAAG TGTATTACCA ACCACTGCCA GTCTGAGTGA GATTCTGGAG
    GCTATGAAAC ATCCAACTAC AGGCATCCCT CTCCTGTCAG AGCAGAAGTG CCTTCCTCCT
    GGTTGCTTTA TCAGTGCTGA GGCTGTGCAT TGGCTGGTCA ATAATATTAG CTGGGTGCAA
    AGTCAGGTTA TGGCCATGGA TATCATGCAG AAGATGCTTG AAGAGCAGTA TATCACACAT
    GCTTCAGGAG AGACATTTAG AACTTTCATC TATGGCTTTT ACTTGTACAA GATCCCAGCT
    GACAAGGAGC CTGACAGAGC ATCAAACCTG GTACAGCCAT GCGTGTGGCA TTCCACAGCC
    ATGGAGGATT TCACAGCCTT CCAACGGAAG TGGTTTGAGG TTGCATTGGT GGAGGAAGAG
    CTACTGCACT CCGAGATCCC ACCTTTTCTT TTGCCGTGGT TACCTAGTCG CCCCGCATCT
    TATGCAAGTA GGCACAGCTC CTTTAGCCGC AGCTTCGGAG GAAGGAGCCA GGCCGCTGCT
    CTGCTAGCTG CTACAGTGCC AGAGCAGAGA ACGGTCACTT TAGATGTGGA TGTTAATAAC
    CGCACTGACC GGCTAGAATG GTGCAGCAGC TACTATCACG GAAACTTCTC CTTGACTGCT
    GCTTTTGAGA TCAAACTGCA CTGGATGGCA GCAACTGCAG CAATGCTATT TGAGATGGTT
    CAAGGATGGC ACAGAAAAGC TCCGTCCTGC GGTTTCCTCC TAGTACCTGT TGTAGAAGGC
    CCTTTTTCTC TCCCTAGTTA CTTGTATGGG GATCCTTTGC GTGCTCAGCT TTTTATTCCA
    CTTAATATCA GCTGCTTAGT AAAAGCTGAG AATGACCATC TTTTTGAAGG TTTTGAACCA
    GAAACTTACT GGGAACGAAT GTACTTGCTA CAGGAAGTAA TAGCTTATAG ATTTGGCTTT
    ATTCAGGACA AATATTCAGC ATCTGCATTT AACTTCCCAT CAGAGAATAA ACCACAATAT
    ATACATGTGA CAGGTACTGT ATTCTTACAG CTGCCTTACT CAAAGCGGAA AGGACAGCAG
    AGACGTAGGA GGAATTCGAC CAGCTCTTCT ACTCAGAACC TTTTTGGAGA AGAACGCATA
    GGGTATAATT GGGCCTACAA TACCATGCTG ACTAAAACGT GGCGCTCCAT TGGTACAGGA
    GATGAGAGGT TTGCTGACCG ACTGCTTAAA GACTTTACTG ACTTCTGTGC CAACAGAGAT
    AACAGGTTAG GCACATTCTG GGCAAACTGC TTGGAGAAAA TGAACACAAG TGCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_031748034.1
    CDS306..5045
    Translation

    Target ORF information:

    RefSeq Version XM_031892174.1
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_031892174.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTCAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCAGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGTGCCTGG TTTCTGCTGC ACGGTTGGGG TGGATTGGAA GTCCCTCACC
    ACCCCAGCCT GCCTGCCGCT TACCACAGAC TACTTCCCAG ATAGGCAAAG TCTACAAAAT
    GATTACACAG AGGGATGCTA TGACCTTCTG CCAGAAGCTG ACCTTGACAG ACGAGATGAA
    GAGGGAGCTC AGATGACTGC ACAACAAGTT TTTGAAGAGT TTATCTGCCA ACGTCTTATG
    CAAGGTTATC AAATTATTGT TCAGCCAAAA CACCCAAAAG CCATCTCCTC TGTTCCTCCT
    CCACTCAGCA GCAGCCCGCT TTACAGAAGA GGGCTGGTGT CAAGGAACAG AGCTGAAGAG
    GAGAACCAGT ACTGGCTGAG TATGGGCCGA ACCTTCCATA AGGTGACACT AAAGGACAAG
    ATAATAACAG TCACTAGATA TCTACCCAAG TATCCATATG AATCTGCCCA AATCAGCTAC
    ATGTACAGTC TGTGCCCATC ACACTCTGAG TGTGATTTTG TGTCCAGCTG GGTGGAATTC
    TCCCATGAGC GACTGGAGGA TTATAAATGG AACTATTTGG ATCAGTATAT ATGCTCAGCT
    GGCTCTGAAG ATTTCAGTCT CATCGAATCC CTAAAGTTTT GGCGCACACG TTTCCTTTTG
    CTCCCTTCTT GTGTGGGACC CACAAAACGC ATCACTGAGG GGGAACAACG CTGTGATATA
    TATGGTGAAC GCCCTCGATC AGAACAAGAG GAAGCACAGT TGTTAGACGG CTTTTTACGC
    TTTGTGGAGG GGCTAAACCG AATCCGTCGA CGCCACCGTT CTGATCGCAT GATCCGGAAA
    GGAGCAGGTA TGAAGGGCCT GCAATTGCCT TCTCATCCGC CAGAACCTCC AGGTCCACCT
    ATAGGGAAGA AAGGAACATC AGCACTATCA GCATTGCTTG AGATGGAAGC TAATCAAAAG
    AGCCTGGGGG AGCAGCAAGC CCTTGTACAT GCTGCAAAAT TGTCATCTCA GGTCTCAGAC
    CCTGCGGTAA CTCCCACTTA TGTGGACAGT CCTCGTAAGG ATGGGGCCTT CTTTGTGGAG
    TTTGTACGCA GTCCACGCAC AGCGCCTTCC TTCACTCCTC AGATCTCCAT TGACCAAGGA
    ACTTCTTTAG TGACTGATGG AAATAATGCC ATGCCTAACC CTACCCCTTC TGAAAGAATA
    CAGAGTCAGG GTGCAACCTC TGTAGATCAG CCCCCCACTT CAACTGAATG CAGTGCACAG
    CAGATATCTG TGGGTCCAAG TGTATTACCA ACCACTGCCA GTCTGAGTGA GATTCTGGAG
    GCTATGAAAC ATCCAACTAC AGGCATCCCT CTCCTGTCAG AGCAGAAGTG CCTTCCTCCT
    GGTTGCTTTA TCAGTGCTGA GGCTGTGCAT TGGCTGGTCA ATAATATTAG CTGGGTGCAA
    AGTCAGGTTA TGGCCATGGA TATCATGCAG AAGATGCTTG AAGAGCAGTA TATCACACAT
    GCTTCAGGAG AGACATTTAG AACTTTCATC TATGGCTTTT ACTTGTACAA GATCCCAGCT
    GACAAGGAGC CTGACAGAGC ATCAAACCTG GTACAGCCAT GCGTGTGGCA TTCCACAGCC
    ATGGAGGATT TCACAGCCTT CCAACGGAAG TGGTTTGAGG TTGCATTGGT GGAGGAAGAG
    CTACTGCACT CCGAGATCCC ACCTTTTCTT TTGCCGTGGT TACCTAGTCG CCCCGCATCT
    TATGCAAGTA GGCACAGCTC CTTTAGCCGC AGCTTCGGAG GAAGGAGCCA GGCCGCTGCT
    CTGCTAGCTG CTACAGTGCC AGAGCAGAGA ACGGTCACTT TAGATGTGGA TGTTAATAAC
    CGCACTGACC GGCTAGAATG GTGCAGCAGC TACTATCACG GAAACTTCTC CTTGACTGCT
    GCTTTTGAGA TCAAACTGCA CTGGATGGCA GCAACTGCAG CAATGCTATT TGAGATGGTT
    CAAGGATGGC ACAGAAAAGC TCCGTCCTGC GGTTTCCTCC TAGTACCTGT TGTAGAAGGC
    CCTTTTTCTC TCCCTAGTTA CTTGTATGGG GATCCTTTGC GTGCTCAGCT TTTTATTCCA
    CTTAATATCA GCTGCTTAGT AAAAGCTGAG AATGACCATC TTTTTGAAGG TTTTGAACCA
    GAAACTTACT GGGAACGAAT GTACTTGCTA CAGGAAGTAA TAGCTTATAG ATTTGGCTTT
    ATTCAGGACA AATATTCAGC ATCTGCATTT AACTTCCCAT CAGAGAATAA ACCACAATAT
    ATACATGTGA CAGGTACTGT ATTCTTACAG CTGCCTTACT CAAAGCGGAA AGGACAGCAG
    AGACGTAGGA GGAATTCGAC CAGCTCTTCT ACTCAGAACC TTTTTGGAGA AGAACGCATA
    GGGTATAATT GGGCCTACAA TACCATGCTG ACTAAAACGT GGCGCTCCAT TGGTACAGGA
    GATGAGAGGT TTGCTGACCG ACTGCTTAAA GACTTTACTG ACTTCTGTGC CAACAGAGAT
    AACAGGTTAG GCACATTCTG GGCAAACTGC TTGGAGAAAA TGAACACAAG TGCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03643
    Clone ID Related Accession (Same CDS sequence) XM_004910591.3
    Accession Version XM_004910591.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_004910591.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT GCAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCG TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAAGTAGGC ACAGCTCCTT TAGCCGCAGC
    TTCGGAGGAA GGAGCCAGGC CGCTGCTCTG CTAGCTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG TAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAGTATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_004910648.1
    CDS48..4874
    Translation

    Target ORF information:

    RefSeq Version XM_004910591.3
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_004910591.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT GCAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCG TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAAGTAGGC ACAGCTCCTT TAGCCGCAGC
    TTCGGAGGAA GGAGCCAGGC CGCTGCTCTG CTAGCTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG TAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAGTATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa03644
    Clone ID Related Accession (Same CDS sequence) XM_002931918.4
    Accession Version XM_002931918.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4800bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_002931918.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATGCCTGG TTTCTGCTGC ACGGTTGGGG TGGATTGGAA GTCCCTCACC
    ACCCCAGCCT GCCTGCCGCT TACCACAGAC TACTTCCCAG ATAGGCAAAG TCTGCAAAAT
    GATTACACAG AGGGATGCTA TGACCTTCTG CCAGAAGCTG ACCTTGACAG ACGAGATGAA
    GAGGGAGCTC AGATGACTGC ACAACAAGTT TTTGAAGAGT TTATCTGCCA ACGTCTTATG
    CAAGGTTATC AAATTATTGT TCAGCCAAAA CACCCAAAAG CCATCTCCTC TGTTCCTCCT
    CCACTCAGCA GCAGCCCGCT TTACAGAAGA GGGCTGGTGT CAAGGAACAG AGCTGAAGAG
    GAGAACCAGT ACTGGCTGAG TATGGGCCGA ACCTTCCATA AGGTGACACT AAAGGACAAG
    ATAATAACAG TCACTAGATA TCTACCCAAG TATCCATATG AATCTGCCCA AATCAGCTAC
    ATGTACAGTC TGTGCCCATC ACACTCTGAG TGTGATTTTG TGTCCAGCTG GGTGGAATTC
    TCCCATGAGC GACTGGAGGA TTATAAATGG AACTATTTGG ATCAGTATAT ATGCTCAGCT
    GGCTCTGAAG ATTTCAGTCT CATCGAATCC CTAAAGTTTT GGCGCACACG TTTCCTTTTG
    CTCCCTTCTT GTGTGGGACC CACAAAACGC ATCACTGAGG GGGAACAACG CTGTGATATA
    TATGGTGAAC GCCCTCGATC AGAACAAGAG GAAGCACAGT TGTTAGACGG CTTTTTACGC
    TTTGTGGAGG GGCTAAACCG AATCCGTCGA CGCCACCGTT CTGATCGCAT GATCCGGAAA
    GGAGCAGGTA TGAAGGGCCT GCAATTGCCT TCTCATCCGC CAGAACCTCC AGGTCCACCT
    ATAGGGAAGA AAGGAACATC AGCACTATCA GCATTGCTTG AGATGGAAGC TAATCAAAAG
    AGCCTGGGGG AGCAGCAAGC CCTTGTACAT GCTGCAAAAT TGTCATCTCA GGTCTCAGAC
    CCTGCGGTAA CTCCCACTTA TGTGGACAGT CCTCGTAAGG ATGGGGCCTT CTTTGTGGAG
    TTTGTACGCA GTCCACGCAC AGCGCCTTCC TTCACTCCTC AGATCTCCAT TGACCAAGGA
    ACTTCTTTAG TGACTGATGG AAATAATGCC ATGCCTAACC CTACCCCTTC TGAAAGAATA
    CAGAGTCAGG GTGCAACCTC TGTAGATCAG CCCCCCACTT CAACTGAATG CAGTGCACAG
    CAGATATCTG TGGGTCCAAG TGTATTACCA ACCACTGCCA GTCTGAGTGA GATTCTGGAG
    GCTATGAAAC ATCCAACTAC AGGCATCCCT CTCCTGTCAG AGCAGAAGTG CCTTCCTCCT
    GGTTGCTTTA TCAGTGCTGA GGCTGTGCAT TGGCTGGTCA ATAATATTAG CTGGGTGCAA
    AGTCAGGTTA TGGCCATGGA TATCATGCAG AAGATGCTTG AAGAGCAGTA TATCACACAT
    GCGTCAGGAG AGACATTTAG AACTTTCATC TATGGCTTTT ACTTGTACAA GATCCCAGCT
    GACAAGGAGC CTGACAGAGC ATCAAACCTG GTACAGCCAT GCGTGTGGCA TTCCACAGCC
    ATGGAGGATT TCACAGCCTT CCAACGGAAG TGGTTTGAGG TTGCATTGGT GGAGGAAGAG
    CTACTGCACT CCGAGATCCC ACCTTTTCTT TTGCCGTGGT TACCTAGTCG CCCCGCATCT
    TATGCAAGTA GGCACAGCTC CTTTAGCCGC AGCTTCGGAG GAAGGAGCCA GGCCGCTGCT
    CTGCTAGCTG CTACAGTGCC AGAGCAGAGA ACGGTCACTT TAGATGTGGA TGTTAATAAC
    CGCACTGACC GGCTAGAATG GTGTAGCAGC TACTATCACG GAAACTTCTC CTTGACTGCT
    GCTTTTGAGA TCAAACTGCA CTGGATGGCA GCAACTGCAG CAATGCTATT TGAGATGGTT
    CAAGGATGGC ACAGAAAAGC TCCGTCCTGC GGTTTCCTCC TAGTACCTGT TGTAGAAGGC
    CCTTTTTCTC TCCCTAGTTA CTTGTATGGG GATCCTTTGC GTGCTCAGCT TTTTATTCCA
    CTTAATATCA GCTGCTTAGT AAAAGCTGAG AATGACCATC TTTTTGAAGG TTTTGAACCA
    GAAACTTACT GGGAACGAAT GTACTTGCTA CAGGAAGTAA TAGCTTATAG ATTTGGCTTT
    ATTCAGGACA AATATTCAGC ATCTGCATTT AACTTCCCAT CAGAGAATAA ACCACAGTAT
    ATACATGTGA CAGGTACTGT ATTCTTACAG CTGCCTTACT CAAAGCGGAA AGGACAGCAG
    AGACGTAGGA GGAATTCGAC CAGCTCTTCT ACTCAGAACC TTTTTGGAGA AGAACGCATA
    GGGTATAATT GGGCCTACAA TACCATGCTG ACTAAAACGT GGCGCTCCAT TGGTACAGGA
    GATGAGAGGT TTGCTGACCG ACTGCTTAAA GACTTTACTG ACTTCTGTGC CAACAGAGAT
    AACAGGTTAG GCACATTCTG GGCAAACTGC TTGGAGAAAA TGAACACAAG TGCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002931964.2
    CDS48..4847
    Translation

    Target ORF information:

    RefSeq Version XM_002931918.4
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002931918.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATGCCTGG TTTCTGCTGC ACGGTTGGGG TGGATTGGAA GTCCCTCACC
    ACCCCAGCCT GCCTGCCGCT TACCACAGAC TACTTCCCAG ATAGGCAAAG TCTGCAAAAT
    GATTACACAG AGGGATGCTA TGACCTTCTG CCAGAAGCTG ACCTTGACAG ACGAGATGAA
    GAGGGAGCTC AGATGACTGC ACAACAAGTT TTTGAAGAGT TTATCTGCCA ACGTCTTATG
    CAAGGTTATC AAATTATTGT TCAGCCAAAA CACCCAAAAG CCATCTCCTC TGTTCCTCCT
    CCACTCAGCA GCAGCCCGCT TTACAGAAGA GGGCTGGTGT CAAGGAACAG AGCTGAAGAG
    GAGAACCAGT ACTGGCTGAG TATGGGCCGA ACCTTCCATA AGGTGACACT AAAGGACAAG
    ATAATAACAG TCACTAGATA TCTACCCAAG TATCCATATG AATCTGCCCA AATCAGCTAC
    ATGTACAGTC TGTGCCCATC ACACTCTGAG TGTGATTTTG TGTCCAGCTG GGTGGAATTC
    TCCCATGAGC GACTGGAGGA TTATAAATGG AACTATTTGG ATCAGTATAT ATGCTCAGCT
    GGCTCTGAAG ATTTCAGTCT CATCGAATCC CTAAAGTTTT GGCGCACACG TTTCCTTTTG
    CTCCCTTCTT GTGTGGGACC CACAAAACGC ATCACTGAGG GGGAACAACG CTGTGATATA
    TATGGTGAAC GCCCTCGATC AGAACAAGAG GAAGCACAGT TGTTAGACGG CTTTTTACGC
    TTTGTGGAGG GGCTAAACCG AATCCGTCGA CGCCACCGTT CTGATCGCAT GATCCGGAAA
    GGAGCAGGTA TGAAGGGCCT GCAATTGCCT TCTCATCCGC CAGAACCTCC AGGTCCACCT
    ATAGGGAAGA AAGGAACATC AGCACTATCA GCATTGCTTG AGATGGAAGC TAATCAAAAG
    AGCCTGGGGG AGCAGCAAGC CCTTGTACAT GCTGCAAAAT TGTCATCTCA GGTCTCAGAC
    CCTGCGGTAA CTCCCACTTA TGTGGACAGT CCTCGTAAGG ATGGGGCCTT CTTTGTGGAG
    TTTGTACGCA GTCCACGCAC AGCGCCTTCC TTCACTCCTC AGATCTCCAT TGACCAAGGA
    ACTTCTTTAG TGACTGATGG AAATAATGCC ATGCCTAACC CTACCCCTTC TGAAAGAATA
    CAGAGTCAGG GTGCAACCTC TGTAGATCAG CCCCCCACTT CAACTGAATG CAGTGCACAG
    CAGATATCTG TGGGTCCAAG TGTATTACCA ACCACTGCCA GTCTGAGTGA GATTCTGGAG
    GCTATGAAAC ATCCAACTAC AGGCATCCCT CTCCTGTCAG AGCAGAAGTG CCTTCCTCCT
    GGTTGCTTTA TCAGTGCTGA GGCTGTGCAT TGGCTGGTCA ATAATATTAG CTGGGTGCAA
    AGTCAGGTTA TGGCCATGGA TATCATGCAG AAGATGCTTG AAGAGCAGTA TATCACACAT
    GCGTCAGGAG AGACATTTAG AACTTTCATC TATGGCTTTT ACTTGTACAA GATCCCAGCT
    GACAAGGAGC CTGACAGAGC ATCAAACCTG GTACAGCCAT GCGTGTGGCA TTCCACAGCC
    ATGGAGGATT TCACAGCCTT CCAACGGAAG TGGTTTGAGG TTGCATTGGT GGAGGAAGAG
    CTACTGCACT CCGAGATCCC ACCTTTTCTT TTGCCGTGGT TACCTAGTCG CCCCGCATCT
    TATGCAAGTA GGCACAGCTC CTTTAGCCGC AGCTTCGGAG GAAGGAGCCA GGCCGCTGCT
    CTGCTAGCTG CTACAGTGCC AGAGCAGAGA ACGGTCACTT TAGATGTGGA TGTTAATAAC
    CGCACTGACC GGCTAGAATG GTGTAGCAGC TACTATCACG GAAACTTCTC CTTGACTGCT
    GCTTTTGAGA TCAAACTGCA CTGGATGGCA GCAACTGCAG CAATGCTATT TGAGATGGTT
    CAAGGATGGC ACAGAAAAGC TCCGTCCTGC GGTTTCCTCC TAGTACCTGT TGTAGAAGGC
    CCTTTTTCTC TCCCTAGTTA CTTGTATGGG GATCCTTTGC GTGCTCAGCT TTTTATTCCA
    CTTAATATCA GCTGCTTAGT AAAAGCTGAG AATGACCATC TTTTTGAAGG TTTTGAACCA
    GAAACTTACT GGGAACGAAT GTACTTGCTA CAGGAAGTAA TAGCTTATAG ATTTGGCTTT
    ATTCAGGACA AATATTCAGC ATCTGCATTT AACTTCCCAT CAGAGAATAA ACCACAGTAT
    ATACATGTGA CAGGTACTGT ATTCTTACAG CTGCCTTACT CAAAGCGGAA AGGACAGCAG
    AGACGTAGGA GGAATTCGAC CAGCTCTTCT ACTCAGAACC TTTTTGGAGA AGAACGCATA
    GGGTATAATT GGGCCTACAA TACCATGCTG ACTAAAACGT GGCGCTCCAT TGGTACAGGA
    GATGAGAGGT TTGCTGACCG ACTGCTTAAA GACTTTACTG ACTTCTGTGC CAACAGAGAT
    AACAGGTTAG GCACATTCTG GGCAAACTGC TTGGAGAAAA TGAACACAAG TGCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37170
    Clone ID Related Accession (Same CDS sequence) XM_012969655.2
    Accession Version XM_012969655.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4902bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969655.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GGATGGGGCC TTCTTTGTGG AGTTTGTACG CAGTCCACGC
    ACAGCGCCTT CCTTCACTCC TCAGATCTCC ATTGACCAAG GAACTTCTTT AGTGACTGAT
    GGAAATAATG CCATGCCTAA CCCTACCCCT TCTGAAAGAA TACAGAGTCA GGGTGCAACC
    TCTGTAGATC AGCCCCCCAC TTCAACTGAA TGCAGTGCAC AGCAGATATC TGTGGGTCCA
    AGTGTATTAC CAACCACTGC CAGTCTGAGT GAGATTCTGG AGGCTATGAA ACATCCAACT
    ACAGGCATCC CTCTCCTGTC AGAGCAGAAG TGCCTTCCTC CTGGTTGCTT TATCAGTGCT
    GAGGCTGTGC ATTGGCTGGT CAATAATATT AGCTGGGTGC AAAGTCAGGT TATGGCCATG
    GATATCATGC AGAAGATGCT TGAAGAGCAG TATATCACAC ATGCGTCAGG AGAGACATTT
    AGAACTTTCA TCTATGGCTT TTACTTGTAC AAGATCCCAG CTGACAAGGA GCCTGACAGA
    GCATCAAACC TGGTACAGCC ATGCGTGTGG CATTCCACAG CCATGGAGGA TTTCACAGCC
    TTCCAACGGA AGTGGTTTGA GGTTGCATTG GTGGAGGAAG AGCTACTGCA CTCCGAGATC
    CCACCTTTTC TTTTGCCGTG GTTACCTAGT CGCCCCGCAT CTTATGCAAG TAGGCACAGC
    TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC CAGGCCGCTG CTCTGCTAGC TGCTACAGTG
    CCAGAGCAGA GAACGGTCAC TTTAGATGTG GATGTTAATA ACCGCACTGA CCGGCTAGAA
    TGGTGTAGCA GCTACTATCA CGGAAACTTC TCCTTGACTG CTGCTTTTGA GATCAAACTG
    CACTGGATGG CAGCAACTGC AGCAATGCTA TTTGAGATGG TTCAAGGATG GCACAGAAAA
    GCTCCGTCCT GCGGTTTCCT CCTAGTACCT GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT
    TACTTGTATG GGGATCCTTT GCGTGCTCAG CTTTTTATTC CACTTAATAT CAGCTGCTTA
    GTAAAAGCTG AGAATGACCA TCTTTTTGAA GGTTTTGAAC CAGAAACTTA CTGGGAACGA
    ATGTACTTGC TACAGGAAGT AATAGCTTAT AGATTTGGCT TTATTCAGGA CAAATATTCA
    GCATCTGCAT TTAACTTCCC ATCAGAGAAT AAACCACAGT ATATACATGT GACAGGTACT
    GTATTCTTAC AGCTGCCTTA CTCAAAGCGG AAAGGACAGC AGAGACGTAG GAGGAATTCG
    ACCAGCTCTT CTACTCAGAA CCTTTTTGGA GAAGAACGCA TAGGGTATAA TTGGGCCTAC
    AATACCATGC TGACTAAAAC GTGGCGCTCC ATTGGTACAG GAGATGAGAG GTTTGCTGAC
    CGACTGCTTA AAGACTTTAC TGACTTCTGT GCCAACAGAG ATAACAGGTT AGGCACATTC
    TGGGCAAACT GCTTGGAGAA AATGAACACA AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825109.1
    CDS48..4949
    Translation

    Target ORF information:

    RefSeq Version XM_012969655.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969655.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GGATGGGGCC TTCTTTGTGG AGTTTGTACG CAGTCCACGC
    ACAGCGCCTT CCTTCACTCC TCAGATCTCC ATTGACCAAG GAACTTCTTT AGTGACTGAT
    GGAAATAATG CCATGCCTAA CCCTACCCCT TCTGAAAGAA TACAGAGTCA GGGTGCAACC
    TCTGTAGATC AGCCCCCCAC TTCAACTGAA TGCAGTGCAC AGCAGATATC TGTGGGTCCA
    AGTGTATTAC CAACCACTGC CAGTCTGAGT GAGATTCTGG AGGCTATGAA ACATCCAACT
    ACAGGCATCC CTCTCCTGTC AGAGCAGAAG TGCCTTCCTC CTGGTTGCTT TATCAGTGCT
    GAGGCTGTGC ATTGGCTGGT CAATAATATT AGCTGGGTGC AAAGTCAGGT TATGGCCATG
    GATATCATGC AGAAGATGCT TGAAGAGCAG TATATCACAC ATGCGTCAGG AGAGACATTT
    AGAACTTTCA TCTATGGCTT TTACTTGTAC AAGATCCCAG CTGACAAGGA GCCTGACAGA
    GCATCAAACC TGGTACAGCC ATGCGTGTGG CATTCCACAG CCATGGAGGA TTTCACAGCC
    TTCCAACGGA AGTGGTTTGA GGTTGCATTG GTGGAGGAAG AGCTACTGCA CTCCGAGATC
    CCACCTTTTC TTTTGCCGTG GTTACCTAGT CGCCCCGCAT CTTATGCAAG TAGGCACAGC
    TCCTTTAGCC GCAGCTTCGG AGGAAGGAGC CAGGCCGCTG CTCTGCTAGC TGCTACAGTG
    CCAGAGCAGA GAACGGTCAC TTTAGATGTG GATGTTAATA ACCGCACTGA CCGGCTAGAA
    TGGTGTAGCA GCTACTATCA CGGAAACTTC TCCTTGACTG CTGCTTTTGA GATCAAACTG
    CACTGGATGG CAGCAACTGC AGCAATGCTA TTTGAGATGG TTCAAGGATG GCACAGAAAA
    GCTCCGTCCT GCGGTTTCCT CCTAGTACCT GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT
    TACTTGTATG GGGATCCTTT GCGTGCTCAG CTTTTTATTC CACTTAATAT CAGCTGCTTA
    GTAAAAGCTG AGAATGACCA TCTTTTTGAA GGTTTTGAAC CAGAAACTTA CTGGGAACGA
    ATGTACTTGC TACAGGAAGT AATAGCTTAT AGATTTGGCT TTATTCAGGA CAAATATTCA
    GCATCTGCAT TTAACTTCCC ATCAGAGAAT AAACCACAGT ATATACATGT GACAGGTACT
    GTATTCTTAC AGCTGCCTTA CTCAAAGCGG AAAGGACAGC AGAGACGTAG GAGGAATTCG
    ACCAGCTCTT CTACTCAGAA CCTTTTTGGA GAAGAACGCA TAGGGTATAA TTGGGCCTAC
    AATACCATGC TGACTAAAAC GTGGCGCTCC ATTGGTACAG GAGATGAGAG GTTTGCTGAC
    CGACTGCTTA AAGACTTTAC TGACTTCTGT GCCAACAGAG ATAACAGGTT AGGCACATTC
    TGGGCAAACT GCTTGGAGAA AATGAACACA AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37171
    Clone ID Related Accession (Same CDS sequence) XM_012969676.2
    Accession Version XM_012969676.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4827bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGGACCA ACAGAGTATA CAAGCTAGTC ATCCACAAGA AAGGCTTTGG GGGCAGTGAT 
    GATGAGTTGG TGATAAACCC CAAGGTCTTT CCATATATTA ACCTGGGGGA CATTGTTGAA
    ATTGCTCACC CAAATGATGA ATACAGTCCC CTTCTCCTGC AGGTGAAGTC ACTAAAAGAA
    GATTTGCAAA AAGAAACTGT TAGTATTGAC CAAACCGTAG CCCAAGTGTT TCGCCTACGG
    CCATACCAGG ATGTTTATGT CAATGTTGTT GACCCTAAGG ATGTGACTCT TGATCTTGTG
    GAGTTAACAT TTAAGGACCA GTATATAGGT CGTGGAGATA TGTGGAGGCT AAAAAAGAGT
    TTGGTCAGCT CCTGTGCTTA CATAACTCAG AAAGTGGAGT TTGCTGGAAT TCGAGCACAA
    GTTGGAGAAT TATGGGTTAA GAGCGAGAAA GTCACCTGTG GGTACATCAG TGAAGACACA
    CGAGTTGTAT TCAGGTCCAC ATCGGCCATG GTGTATATAT TTATACAAAT GAGCAGTGAA
    CTGTGGGACT TTGATATCTA TGGTGATCTG TATTTTGAGA AGGCAGTAAA TGGATTTCTG
    GCTGATCTCT TTAACAAATG GAAGGACAAG AACTGCAGTC ACGAGGTTAC AGTGGTTCTA
    TTCTCTAGGA CCTTTTATGA TGCAAAGAAT ATTGAGGACT TTCCAGAAGC ACACCGTAAT
    GCGTCCGTCA TGCAAGATCA CAAGGGCAGA TTCTATGAAG ACTTCTACAA GGTGGTTGTA
    CAAAATGAAA GGCGAGAAGA ATGGACTTCA TTGCTAGTCA CTATTAAGAA ACTTTTTATT
    CAGTACCCAG TGCTTGTACG ACTGGAAAAT GCAGAGGGCT TTCCCCCAGG GCATAATTCT
    ACTTCAGATC AAGGAAATTA TCTAGAGGCC ATTAACCTGT CCTTTAATGT GTTTGACAAG
    CATTATATAA ATCGCAACTT TGACCGAACT GGTCAGATGT CTGTTGTGAT CACTCCAGGT
    GTTGGTGTTT TCCAAGTGGA TCGTTCACTA ATGATTCTGA CCAAGCAGCG AATGATTGAT
    AATGGGATTG GTGTGGATCT GGTATGTATG GGAGAGCAGC CGCTTCATGC TGTTCCACTT
    TTCAAGCTGC ACAATCGTTC CAGTCCAGGA GATTCACGTC TTGGAGATGA TTATAACATC
    CCTCACTGGA TTAACCATAG CTTCTACACA TCGCAGAGCC AGCCTCTAAA TAACAGCTTT
    ACCCCACGCA TCAAACTGGC ACCTCGCAAG ACAAATAGTG AGAAAGGAAG GACTGGAAAA
    GACCCATCCA TAGGAAGCCC CAAAGAGACA GAGAATGCTC TTCCCATACA AGTAGACTAC
    GATGCTTATG ACTCACAGGT CTTCCGATTA CCCGGTCCAT CCAGGGCACA GCGAAGCATA
    ACTTTCAGGG CTTTTTGGGC ATTTTGGAGA TTTCTTCTTT TCCTCCAGTC GCTGCCTTGG
    CAAACCATAG AAGAGGGAAA GAAGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT GCAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCG TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAAGTAGGC ACAGCTCCTT TAGCCGCAGC
    TTCGGAGGAA GGAGCCAGGC CGCTGCTCTG CTAGCTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG TAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAGTATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825130.1
    CDS139..4965
    Translation

    Target ORF information:

    RefSeq Version XM_012969676.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969676.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGGACCA ACAGAGTATA CAAGCTAGTC ATCCACAAGA AAGGCTTTGG GGGCAGTGAT 
    GATGAGTTGG TGATAAACCC CAAGGTCTTT CCATATATTA ACCTGGGGGA CATTGTTGAA
    ATTGCTCACC CAAATGATGA ATACAGTCCC CTTCTCCTGC AGGTGAAGTC ACTAAAAGAA
    GATTTGCAAA AAGAAACTGT TAGTATTGAC CAAACCGTAG CCCAAGTGTT TCGCCTACGG
    CCATACCAGG ATGTTTATGT CAATGTTGTT GACCCTAAGG ATGTGACTCT TGATCTTGTG
    GAGTTAACAT TTAAGGACCA GTATATAGGT CGTGGAGATA TGTGGAGGCT AAAAAAGAGT
    TTGGTCAGCT CCTGTGCTTA CATAACTCAG AAAGTGGAGT TTGCTGGAAT TCGAGCACAA
    GTTGGAGAAT TATGGGTTAA GAGCGAGAAA GTCACCTGTG GGTACATCAG TGAAGACACA
    CGAGTTGTAT TCAGGTCCAC ATCGGCCATG GTGTATATAT TTATACAAAT GAGCAGTGAA
    CTGTGGGACT TTGATATCTA TGGTGATCTG TATTTTGAGA AGGCAGTAAA TGGATTTCTG
    GCTGATCTCT TTAACAAATG GAAGGACAAG AACTGCAGTC ACGAGGTTAC AGTGGTTCTA
    TTCTCTAGGA CCTTTTATGA TGCAAAGAAT ATTGAGGACT TTCCAGAAGC ACACCGTAAT
    GCGTCCGTCA TGCAAGATCA CAAGGGCAGA TTCTATGAAG ACTTCTACAA GGTGGTTGTA
    CAAAATGAAA GGCGAGAAGA ATGGACTTCA TTGCTAGTCA CTATTAAGAA ACTTTTTATT
    CAGTACCCAG TGCTTGTACG ACTGGAAAAT GCAGAGGGCT TTCCCCCAGG GCATAATTCT
    ACTTCAGATC AAGGAAATTA TCTAGAGGCC ATTAACCTGT CCTTTAATGT GTTTGACAAG
    CATTATATAA ATCGCAACTT TGACCGAACT GGTCAGATGT CTGTTGTGAT CACTCCAGGT
    GTTGGTGTTT TCCAAGTGGA TCGTTCACTA ATGATTCTGA CCAAGCAGCG AATGATTGAT
    AATGGGATTG GTGTGGATCT GGTATGTATG GGAGAGCAGC CGCTTCATGC TGTTCCACTT
    TTCAAGCTGC ACAATCGTTC CAGTCCAGGA GATTCACGTC TTGGAGATGA TTATAACATC
    CCTCACTGGA TTAACCATAG CTTCTACACA TCGCAGAGCC AGCCTCTAAA TAACAGCTTT
    ACCCCACGCA TCAAACTGGC ACCTCGCAAG ACAAATAGTG AGAAAGGAAG GACTGGAAAA
    GACCCATCCA TAGGAAGCCC CAAAGAGACA GAGAATGCTC TTCCCATACA AGTAGACTAC
    GATGCTTATG ACTCACAGGT CTTCCGATTA CCCGGTCCAT CCAGGGCACA GCGAAGCATA
    ACTTTCAGGG CTTTTTGGGC ATTTTGGAGA TTTCTTCTTT TCCTCCAGTC GCTGCCTTGG
    CAAACCATAG AAGAGGGAAA GAAGGCAGTG CGAGAGACAC AAGTTCGAAA AAGTTCTTGT
    TCCTATGACA CATCCTGTAG CCCAGTCAGT CGGCCATTTA CAGCAGAGGA AGGCCGTAGC
    CAAAATTCAG ATGAGAGCTC TTTGGGAAAA CTCTCCAACA TCCTACTTAT CCCAAGGCCA
    CATCTGCATC AATATGAGGC CAGCAGCTCT TTGGGTTATA CTAGCACAAG AGATTTCTTG
    GAGAGGATGA TTGAATCCCA ACAGCGAGAC TCAAGTGCTC CTGGCAGATT CCATGTGGGC
    AGTGCAGAAT CCATGTTACA CGTCCGACCT GGGGGATACC ACCCGCAGAG AGCACTCATC
    AACCCATTTG CACCTTCACG TATGCCAATG AAACTGACAT CAAATCGCAG GAGGTGGATG
    CACACATTTC CAGTGGGCCC ATCTGGAGAA GCAATACAAA TACATCACCA AACTCGTCAG
    AATATGGCAG AGCTACAGGG TAGTGGCATG CGTGAGGCAT CTCATTCTTC TGCAGAGTTG
    CTAGAATTGG CTTACCATGA AGCCACTGGC AGACACAGTG GCCCTCGCAC CCCTGGGGAA
    AATGGCTCTT GTGGGAGCAA TGAAGAATTC TCCTGCAACA CACCCATCAA TGGGCAGAGG
    AACCACAATA CAGGGTCTAA TCACAGGGAG AAAGAGTCCT CTGATGACAG CGTACCATGC
    TGCCCAGAAC CCATTTTGAT GCTGTCTGCT CCCCCCGTAG TGCCTGGTTT CTGCTGCACG
    GTTGGGGTGG ATTGGAAGTC CCTCACCACC CCAGCCTGCC TGCCGCTTAC CACAGACTAC
    TTCCCAGATA GGCAAAGTCT GCAAAATGAT TACACAGAGG GATGCTATGA CCTTCTGCCA
    GAAGCTGACC TTGACAGACG AGATGAAGAG GGAGCTCAGA TGACTGCACA ACAAGTTTTT
    GAAGAGTTTA TCTGCCAACG TCTTATGCAA GGTTATCAAA TTATTGTTCA GCCAAAACAC
    CCAAAAGCCA TCTCCTCTGT TCCTCCTCCA CTCAGCAGCA GCCCGCTTTA CAGAAGAGGG
    CTGGTGTCAA GGAACAGAGC TGAAGAGGAG AACCAGTACT GGCTGAGTAT GGGCCGAACC
    TTCCATAAGG TGACACTAAA GGACAAGATA ATAACAGTCA CTAGATATCT ACCCAAGTAT
    CCATATGAAT CTGCCCAAAT CAGCTACATG TACAGTCTGT GCCCATCACA CTCTGAGTGT
    GATTTTGTGT CCAGCTGGGT GGAATTCTCC CATGAGCGAC TGGAGGATTA TAAATGGAAC
    TATTTGGATC AGTATATATG CTCAGCTGGC TCTGAAGATT TCAGTCTCAT CGAATCCCTA
    AAGTTTTGGC GCACACGTTT CCTTTTGCTC CCTTCTTGTG TGGGACCCAC AAAACGCATC
    ACTGAGGGGG AACAACGCTG TGATATATAT GGTGAACGCC CTCGATCAGA ACAAGAGGAA
    GCACAGTTGT TAGACGGCTT TTTACGCTTT GTGGAGGGGC TAAACCGAAT CCGTCGACGC
    CACCGTTCTG ATCGCATGAT CCGGAAAGGA GCAGGTATGA AGGGCCTGCA ATTGCCTTCT
    CATCCGCCAG AACCTCCAGG TCCACCTATA GGGAAGAAAG GAACATCAGC ACTATCAGCA
    TTGCTTGAGA TGGAAGCTAA TCAAAAGAGC CTGGGGGAGC AGCAAGCCCT TGTACATGCT
    GCAAAATTGT CATCTCAGGT CTCAGACCCT GCGGTAACTC CCACTTATGT GGACAGTCCT
    CGTAAGGATG GGGCCTTCTT TGTGGAGTTT GTACGCAGTC CACGCACAGC GCCTTCCTTC
    ACTCCTCAGA TCTCCATTGA CCAAGGAACT TCTTTAGTGA CTGATGGAAA TAATGCCATG
    CCTAACCCTA CCCCTTCTGA AAGAATACAG AGTCAGGGTG CAACCTCTGT AGATCAGCCC
    CCCACTTCAA CTGAATGCAG TGCACAGCAG ATATCTGTGG GTCCAAGTGT ATTACCAACC
    ACTGCCAGTC TGAGTGAGAT TCTGGAGGCT ATGAAACATC CAACTACAGG CATCCCTCTC
    CTGTCAGAGC AGAAGTGCCT TCCTCCTGGT TGCTTTATCA GTGCTGAGGC TGTGCATTGG
    CTGGTCAATA ATATTAGCTG GGTGCAAAGT CAGGTTATGG CCATGGATAT CATGCAGAAG
    ATGCTTGAAG AGCAGTATAT CACACATGCG TCAGGAGAGA CATTTAGAAC TTTCATCTAT
    GGCTTTTACT TGTACAAGAT CCCAGCTGAC AAGGAGCCTG ACAGAGCATC AAACCTGGTA
    CAGCCATGCG TGTGGCATTC CACAGCCATG GAGGATTTCA CAGCCTTCCA ACGGAAGTGG
    TTTGAGGTTG CATTGGTGGA GGAAGAGCTA CTGCACTCCG AGATCCCACC TTTTCTTTTG
    CCGTGGTTAC CTAGTCGCCC CGCATCTTAT GCAAGTAGGC ACAGCTCCTT TAGCCGCAGC
    TTCGGAGGAA GGAGCCAGGC CGCTGCTCTG CTAGCTGCTA CAGTGCCAGA GCAGAGAACG
    GTCACTTTAG ATGTGGATGT TAATAACCGC ACTGACCGGC TAGAATGGTG TAGCAGCTAC
    TATCACGGAA ACTTCTCCTT GACTGCTGCT TTTGAGATCA AACTGCACTG GATGGCAGCA
    ACTGCAGCAA TGCTATTTGA GATGGTTCAA GGATGGCACA GAAAAGCTCC GTCCTGCGGT
    TTCCTCCTAG TACCTGTTGT AGAAGGCCCT TTTTCTCTCC CTAGTTACTT GTATGGGGAT
    CCTTTGCGTG CTCAGCTTTT TATTCCACTT AATATCAGCT GCTTAGTAAA AGCTGAGAAT
    GACCATCTTT TTGAAGGTTT TGAACCAGAA ACTTACTGGG AACGAATGTA CTTGCTACAG
    GAAGTAATAG CTTATAGATT TGGCTTTATT CAGGACAAAT ATTCAGCATC TGCATTTAAC
    TTCCCATCAG AGAATAAACC ACAGTATATA CATGTGACAG GTACTGTATT CTTACAGCTG
    CCTTACTCAA AGCGGAAAGG ACAGCAGAGA CGTAGGAGGA ATTCGACCAG CTCTTCTACT
    CAGAACCTTT TTGGAGAAGA ACGCATAGGG TATAATTGGG CCTACAATAC CATGCTGACT
    AAAACGTGGC GCTCCATTGG TACAGGAGAT GAGAGGTTTG CTGACCGACT GCTTAAAGAC
    TTTACTGACT TCTGTGCCAA CAGAGATAAC AGGTTAGGCA CATTCTGGGC AAACTGCTTG
    GAGAAAATGA ACACAAGTGC GCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37172
    Clone ID Related Accession (Same CDS sequence) XM_012969661.2
    Accession Version XM_012969661.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969661.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATG CCTGGTTTCT GCTGCACGGT TGGGGTGGAT
    TGGAAGTCCC TCACCACCCC AGCCTGCCTG CCGCTTACCA CAGACTACTT CCCAGATAGG
    CAAAGTCTGC AAAATGATTA CACAGAGGGA TGCTATGACC TTCTGCCAGA AGCTGACCTT
    GACAGACGAG ATGAAGAGGG AGCTCAGATG ACTGCACAAC AAGTTTTTGA AGAGTTTATC
    TGCCAACGTC TTATGCAAGG TTATCAAATT ATTGTTCAGC CAAAACACCC AAAAGCCATC
    TCCTCTGTTC CTCCTCCACT CAGCAGCAGC CCGCTTTACA GAAGAGGGCT GGTGTCAAGG
    AACAGAGCTG AAGAGGAGAA CCAGTACTGG CTGAGTATGG GCCGAACCTT CCATAAGGTG
    ACACTAAAGG ACAAGATAAT AACAGTCACT AGATATCTAC CCAAGTATCC ATATGAATCT
    GCCCAAATCA GCTACATGTA CAGTCTGTGC CCATCACACT CTGAGTGTGA TTTTGTGTCC
    AGCTGGGTGG AATTCTCCCA TGAGCGACTG GAGGATTATA AATGGAACTA TTTGGATCAG
    TATATATGCT CAGCTGGCTC TGAAGATTTC AGTCTCATCG AATCCCTAAA GTTTTGGCGC
    ACACGTTTCC TTTTGCTCCC TTCTTGTGTG GGACCCACAA AACGCATCAC TGAGGGGGAA
    CAACGCTGTG ATATATATGG TGAACGCCCT CGATCAGAAC AAGAGGAAGC ACAGTTGTTA
    GACGGCTTTT TACGCTTTGT GGAGGGGCTA AACCGAATCC GTCGACGCCA CCGTTCTGAT
    CGCATGATCC GGAAAGGAGC AGGTATGAAG GGCCTGCAAT TGCCTTCTCA TCCGCCAGAA
    CCTCCAGGTC CACCTATAGG GAAGAAAGGA ACATCAGCAC TATCAGCATT GCTTGAGATG
    GAAGCTAATC AAAAGAGCCT GGGGGAGCAG CAAGCCCTTG TACATGCTGC AAAATTGTCA
    TCTCAGGTCT CAGACCCTGC GGTAACTCCC ACTTATGTGG ACAGTCCTCG TAAGGATGGG
    GCCTTCTTTG TGGAGTTTGT ACGCAGTCCA CGCACAGCGC CTTCCTTCAC TCCTCAGATC
    TCCATTGACC AAGGAACTTC TTTAGTGACT GATGGAAATA ATGCCATGCC TAACCCTACC
    CCTTCTGAAA GAATACAGAG TCAGGGTGCA ACCTCTGTAG ATCAGCCCCC CACTTCAACT
    GAATGCAGTG CACAGCAGAT ATCTGTGGGT CCAAGTGTAT TACCAACCAC TGCCAGTCTG
    AGTGAGATTC TGGAGGCTAT GAAACATCCA ACTACAGGCA TCCCTCTCCT GTCAGAGCAG
    AAGTGCCTTC CTCCTGGTTG CTTTATCAGT GCTGAGGCTG TGCATTGGCT GGTCAATAAT
    ATTAGCTGGG TGCAAAGTCA GGTTATGGCC ATGGATATCA TGCAGAAGAT GCTTGAAGAG
    CAGTATATCA CACATGCGTC AGGAGAGACA TTTAGAACTT TCATCTATGG CTTTTACTTG
    TACAAGATCC CAGCTGACAA GGAGCCTGAC AGAGCATCAA ACCTGGTACA GCCATGCGTG
    TGGCATTCCA CAGCCATGGA GGATTTCACA GCCTTCCAAC GGAAGTGGTT TGAGGTTGCA
    TTGGTGGAGG AAGAGCTACT GCACTCCGAG ATCCCACCTT TTCTTTTGCC GTGGTTACCT
    AGTCGCCCCG CATCTTATGC AAGTAGGCAC AGCTCCTTTA GCCGCAGCTT CGGAGGAAGG
    AGCCAGGCCG CTGCTCTGCT AGCTGCTACA GTGCCAGAGC AGAGAACGGT CACTTTAGAT
    GTGGATGTTA ATAACCGCAC TGACCGGCTA GAATGGTGTA GCAGCTACTA TCACGGAAAC
    TTCTCCTTGA CTGCTGCTTT TGAGATCAAA CTGCACTGGA TGGCAGCAAC TGCAGCAATG
    CTATTTGAGA TGGTTCAAGG ATGGCACAGA AAAGCTCCGT CCTGCGGTTT CCTCCTAGTA
    CCTGTTGTAG AAGGCCCTTT TTCTCTCCCT AGTTACTTGT ATGGGGATCC TTTGCGTGCT
    CAGCTTTTTA TTCCACTTAA TATCAGCTGC TTAGTAAAAG CTGAGAATGA CCATCTTTTT
    GAAGGTTTTG AACCAGAAAC TTACTGGGAA CGAATGTACT TGCTACAGGA AGTAATAGCT
    TATAGATTTG GCTTTATTCA GGACAAATAT TCAGCATCTG CATTTAACTT CCCATCAGAG
    AATAAACCAC AGTATATACA TGTGACAGGT ACTGTATTCT TACAGCTGCC TTACTCAAAG
    CGGAAAGGAC AGCAGAGACG TAGGAGGAAT TCGACCAGCT CTTCTACTCA GAACCTTTTT
    GGAGAAGAAC GCATAGGGTA TAATTGGGCC TACAATACCA TGCTGACTAA AACGTGGCGC
    TCCATTGGTA CAGGAGATGA GAGGTTTGCT GACCGACTGC TTAAAGACTT TACTGACTTC
    TGTGCCAACA GAGATAACAG GTTAGGCACA TTCTGGGCAA ACTGCTTGGA GAAAATGAAC
    ACAAGTGCGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825115.1
    CDS48..4922
    Translation

    Target ORF information:

    RefSeq Version XM_012969661.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969661.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATG CCTGGTTTCT GCTGCACGGT TGGGGTGGAT
    TGGAAGTCCC TCACCACCCC AGCCTGCCTG CCGCTTACCA CAGACTACTT CCCAGATAGG
    CAAAGTCTGC AAAATGATTA CACAGAGGGA TGCTATGACC TTCTGCCAGA AGCTGACCTT
    GACAGACGAG ATGAAGAGGG AGCTCAGATG ACTGCACAAC AAGTTTTTGA AGAGTTTATC
    TGCCAACGTC TTATGCAAGG TTATCAAATT ATTGTTCAGC CAAAACACCC AAAAGCCATC
    TCCTCTGTTC CTCCTCCACT CAGCAGCAGC CCGCTTTACA GAAGAGGGCT GGTGTCAAGG
    AACAGAGCTG AAGAGGAGAA CCAGTACTGG CTGAGTATGG GCCGAACCTT CCATAAGGTG
    ACACTAAAGG ACAAGATAAT AACAGTCACT AGATATCTAC CCAAGTATCC ATATGAATCT
    GCCCAAATCA GCTACATGTA CAGTCTGTGC CCATCACACT CTGAGTGTGA TTTTGTGTCC
    AGCTGGGTGG AATTCTCCCA TGAGCGACTG GAGGATTATA AATGGAACTA TTTGGATCAG
    TATATATGCT CAGCTGGCTC TGAAGATTTC AGTCTCATCG AATCCCTAAA GTTTTGGCGC
    ACACGTTTCC TTTTGCTCCC TTCTTGTGTG GGACCCACAA AACGCATCAC TGAGGGGGAA
    CAACGCTGTG ATATATATGG TGAACGCCCT CGATCAGAAC AAGAGGAAGC ACAGTTGTTA
    GACGGCTTTT TACGCTTTGT GGAGGGGCTA AACCGAATCC GTCGACGCCA CCGTTCTGAT
    CGCATGATCC GGAAAGGAGC AGGTATGAAG GGCCTGCAAT TGCCTTCTCA TCCGCCAGAA
    CCTCCAGGTC CACCTATAGG GAAGAAAGGA ACATCAGCAC TATCAGCATT GCTTGAGATG
    GAAGCTAATC AAAAGAGCCT GGGGGAGCAG CAAGCCCTTG TACATGCTGC AAAATTGTCA
    TCTCAGGTCT CAGACCCTGC GGTAACTCCC ACTTATGTGG ACAGTCCTCG TAAGGATGGG
    GCCTTCTTTG TGGAGTTTGT ACGCAGTCCA CGCACAGCGC CTTCCTTCAC TCCTCAGATC
    TCCATTGACC AAGGAACTTC TTTAGTGACT GATGGAAATA ATGCCATGCC TAACCCTACC
    CCTTCTGAAA GAATACAGAG TCAGGGTGCA ACCTCTGTAG ATCAGCCCCC CACTTCAACT
    GAATGCAGTG CACAGCAGAT ATCTGTGGGT CCAAGTGTAT TACCAACCAC TGCCAGTCTG
    AGTGAGATTC TGGAGGCTAT GAAACATCCA ACTACAGGCA TCCCTCTCCT GTCAGAGCAG
    AAGTGCCTTC CTCCTGGTTG CTTTATCAGT GCTGAGGCTG TGCATTGGCT GGTCAATAAT
    ATTAGCTGGG TGCAAAGTCA GGTTATGGCC ATGGATATCA TGCAGAAGAT GCTTGAAGAG
    CAGTATATCA CACATGCGTC AGGAGAGACA TTTAGAACTT TCATCTATGG CTTTTACTTG
    TACAAGATCC CAGCTGACAA GGAGCCTGAC AGAGCATCAA ACCTGGTACA GCCATGCGTG
    TGGCATTCCA CAGCCATGGA GGATTTCACA GCCTTCCAAC GGAAGTGGTT TGAGGTTGCA
    TTGGTGGAGG AAGAGCTACT GCACTCCGAG ATCCCACCTT TTCTTTTGCC GTGGTTACCT
    AGTCGCCCCG CATCTTATGC AAGTAGGCAC AGCTCCTTTA GCCGCAGCTT CGGAGGAAGG
    AGCCAGGCCG CTGCTCTGCT AGCTGCTACA GTGCCAGAGC AGAGAACGGT CACTTTAGAT
    GTGGATGTTA ATAACCGCAC TGACCGGCTA GAATGGTGTA GCAGCTACTA TCACGGAAAC
    TTCTCCTTGA CTGCTGCTTT TGAGATCAAA CTGCACTGGA TGGCAGCAAC TGCAGCAATG
    CTATTTGAGA TGGTTCAAGG ATGGCACAGA AAAGCTCCGT CCTGCGGTTT CCTCCTAGTA
    CCTGTTGTAG AAGGCCCTTT TTCTCTCCCT AGTTACTTGT ATGGGGATCC TTTGCGTGCT
    CAGCTTTTTA TTCCACTTAA TATCAGCTGC TTAGTAAAAG CTGAGAATGA CCATCTTTTT
    GAAGGTTTTG AACCAGAAAC TTACTGGGAA CGAATGTACT TGCTACAGGA AGTAATAGCT
    TATAGATTTG GCTTTATTCA GGACAAATAT TCAGCATCTG CATTTAACTT CCCATCAGAG
    AATAAACCAC AGTATATACA TGTGACAGGT ACTGTATTCT TACAGCTGCC TTACTCAAAG
    CGGAAAGGAC AGCAGAGACG TAGGAGGAAT TCGACCAGCT CTTCTACTCA GAACCTTTTT
    GGAGAAGAAC GCATAGGGTA TAATTGGGCC TACAATACCA TGCTGACTAA AACGTGGCGC
    TCCATTGGTA CAGGAGATGA GAGGTTTGCT GACCGACTGC TTAAAGACTT TACTGACTTC
    TGTGCCAACA GAGATAACAG GTTAGGCACA TTCTGGGCAA ACTGCTTGGA GAAAATGAAC
    ACAAGTGCGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37173
    Clone ID Related Accession (Same CDS sequence) XM_012969662.2
    Accession Version XM_012969662.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4842bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969662.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GGATGGGGCC TTCTTTGTGG AGTTTGTACG CAGTCCACGC
    ACAGCGCCTT CCTTCACTCC TCAGATCTCC ATTGACCAAG GAACTTCTTT AGTGACTGAT
    GGAAATAATG CCATGCCTAA CCCTACCCCT TCTGAAAGAA TACAGAGTCA GGGTGCAACC
    TCTGTAGATC AGCCCCCCAC TTCAACTGAA TGCAGTGCAC AGCAGATATC TGTGGGTCCA
    AGTGTATTAC CAACCACTGC CAGTCTGAGT GAGATTCTGG AGGCTATGAA ACATCCAACT
    ACAGGCATCC CTCTCCTGTC AGAGCAGAAG TGCCTTCCTC CTGGTTGCTT TATCAGTGCT
    GAGGCTGTGC ATTGGCTGGT CAATAATATT AGCTGGGTGC AAAGTCAGGT TATGGCCATG
    GATATCATGC AGAAGATGCT TGAAGAGCAG TATATCACAC ATGCGTCAGG AGAGACATTT
    AGAACTTTCA TCTATGGCTT TTACTTGTAC AAGATCCCAG CTGACAAGGA GCCTGACAGA
    GCATCAAACC TGGTACAGCC ATGCGTGTGG CATTCCACAG CCATGGAGGA TTTCACAGCC
    TTCCAACGGA AGTGGTTTGA GGTTGCATTG GTGGAGGAAG AGCTACTGCA CTCCGAGATC
    CCACCTTTTC TTTTGCCGTG GTTACCTAGT CGCCCCGCAT CTTATGCAAC TGCTACAGTG
    CCAGAGCAGA GAACGGTCAC TTTAGATGTG GATGTTAATA ACCGCACTGA CCGGCTAGAA
    TGGTGTAGCA GCTACTATCA CGGAAACTTC TCCTTGACTG CTGCTTTTGA GATCAAACTG
    CACTGGATGG CAGCAACTGC AGCAATGCTA TTTGAGATGG TTCAAGGATG GCACAGAAAA
    GCTCCGTCCT GCGGTTTCCT CCTAGTACCT GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT
    TACTTGTATG GGGATCCTTT GCGTGCTCAG CTTTTTATTC CACTTAATAT CAGCTGCTTA
    GTAAAAGCTG AGAATGACCA TCTTTTTGAA GGTTTTGAAC CAGAAACTTA CTGGGAACGA
    ATGTACTTGC TACAGGAAGT AATAGCTTAT AGATTTGGCT TTATTCAGGA CAAATATTCA
    GCATCTGCAT TTAACTTCCC ATCAGAGAAT AAACCACAGT ATATACATGT GACAGGTACT
    GTATTCTTAC AGCTGCCTTA CTCAAAGCGG AAAGGACAGC AGAGACGTAG GAGGAATTCG
    ACCAGCTCTT CTACTCAGAA CCTTTTTGGA GAAGAACGCA TAGGGTATAA TTGGGCCTAC
    AATACCATGC TGACTAAAAC GTGGCGCTCC ATTGGTACAG GAGATGAGAG GTTTGCTGAC
    CGACTGCTTA AAGACTTTAC TGACTTCTGT GCCAACAGAG ATAACAGGTT AGGCACATTC
    TGGGCAAACT GCTTGGAGAA AATGAACACA AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825116.1
    CDS48..4889
    Translation

    Target ORF information:

    RefSeq Version XM_012969662.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969662.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GGATGGGGCC TTCTTTGTGG AGTTTGTACG CAGTCCACGC
    ACAGCGCCTT CCTTCACTCC TCAGATCTCC ATTGACCAAG GAACTTCTTT AGTGACTGAT
    GGAAATAATG CCATGCCTAA CCCTACCCCT TCTGAAAGAA TACAGAGTCA GGGTGCAACC
    TCTGTAGATC AGCCCCCCAC TTCAACTGAA TGCAGTGCAC AGCAGATATC TGTGGGTCCA
    AGTGTATTAC CAACCACTGC CAGTCTGAGT GAGATTCTGG AGGCTATGAA ACATCCAACT
    ACAGGCATCC CTCTCCTGTC AGAGCAGAAG TGCCTTCCTC CTGGTTGCTT TATCAGTGCT
    GAGGCTGTGC ATTGGCTGGT CAATAATATT AGCTGGGTGC AAAGTCAGGT TATGGCCATG
    GATATCATGC AGAAGATGCT TGAAGAGCAG TATATCACAC ATGCGTCAGG AGAGACATTT
    AGAACTTTCA TCTATGGCTT TTACTTGTAC AAGATCCCAG CTGACAAGGA GCCTGACAGA
    GCATCAAACC TGGTACAGCC ATGCGTGTGG CATTCCACAG CCATGGAGGA TTTCACAGCC
    TTCCAACGGA AGTGGTTTGA GGTTGCATTG GTGGAGGAAG AGCTACTGCA CTCCGAGATC
    CCACCTTTTC TTTTGCCGTG GTTACCTAGT CGCCCCGCAT CTTATGCAAC TGCTACAGTG
    CCAGAGCAGA GAACGGTCAC TTTAGATGTG GATGTTAATA ACCGCACTGA CCGGCTAGAA
    TGGTGTAGCA GCTACTATCA CGGAAACTTC TCCTTGACTG CTGCTTTTGA GATCAAACTG
    CACTGGATGG CAGCAACTGC AGCAATGCTA TTTGAGATGG TTCAAGGATG GCACAGAAAA
    GCTCCGTCCT GCGGTTTCCT CCTAGTACCT GTTGTAGAAG GCCCTTTTTC TCTCCCTAGT
    TACTTGTATG GGGATCCTTT GCGTGCTCAG CTTTTTATTC CACTTAATAT CAGCTGCTTA
    GTAAAAGCTG AGAATGACCA TCTTTTTGAA GGTTTTGAAC CAGAAACTTA CTGGGAACGA
    ATGTACTTGC TACAGGAAGT AATAGCTTAT AGATTTGGCT TTATTCAGGA CAAATATTCA
    GCATCTGCAT TTAACTTCCC ATCAGAGAAT AAACCACAGT ATATACATGT GACAGGTACT
    GTATTCTTAC AGCTGCCTTA CTCAAAGCGG AAAGGACAGC AGAGACGTAG GAGGAATTCG
    ACCAGCTCTT CTACTCAGAA CCTTTTTGGA GAAGAACGCA TAGGGTATAA TTGGGCCTAC
    AATACCATGC TGACTAAAAC GTGGCGCTCC ATTGGTACAG GAGATGAGAG GTTTGCTGAC
    CGACTGCTTA AAGACTTTAC TGACTTCTGT GCCAACAGAG ATAACAGGTT AGGCACATTC
    TGGGCAAACT GCTTGGAGAA AATGAACACA AGTGCGCCTT AG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37174
    Clone ID Related Accession (Same CDS sequence) XM_012969665.2
    Accession Version XM_012969665.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4839bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969665.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CGTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGTAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAGTATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825119.1
    CDS48..4886
    Translation

    Target ORF information:

    RefSeq Version XM_012969665.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969665.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGGG TCTAATCACA GGGAGAAAGA GTCCTCTGAT
    GACAGCGTAC CATGCTGCCC AGAACCCATT TTGATGCTGT CTGCTCCCCC CGTAGTGCCT
    GGTTTCTGCT GCACGGTTGG GGTGGATTGG AAGTCCCTCA CCACCCCAGC CTGCCTGCCG
    CTTACCACAG ACTACTTCCC AGATAGGCAA AGTCTGCAAA ATGATTACAC AGAGGGATGC
    TATGACCTTC TGCCAGAAGC TGACCTTGAC AGACGAGATG AAGAGGGAGC TCAGATGACT
    GCACAACAAG TTTTTGAAGA GTTTATCTGC CAACGTCTTA TGCAAGGTTA TCAAATTATT
    GTTCAGCCAA AACACCCAAA AGCCATCTCC TCTGTTCCTC CTCCACTCAG CAGCAGCCCG
    CTTTACAGAA GAGGGCTGGT GTCAAGGAAC AGAGCTGAAG AGGAGAACCA GTACTGGCTG
    AGTATGGGCC GAACCTTCCA TAAGGTGACA CTAAAGGACA AGATAATAAC AGTCACTAGA
    TATCTACCCA AGTATCCATA TGAATCTGCC CAAATCAGCT ACATGTACAG TCTGTGCCCA
    TCACACTCTG AGTGTGATTT TGTGTCCAGC TGGGTGGAAT TCTCCCATGA GCGACTGGAG
    GATTATAAAT GGAACTATTT GGATCAGTAT ATATGCTCAG CTGGCTCTGA AGATTTCAGT
    CTCATCGAAT CCCTAAAGTT TTGGCGCACA CGTTTCCTTT TGCTCCCTTC TTGTGTGGGA
    CCCACAAAAC GCATCACTGA GGGGGAACAA CGCTGTGATA TATATGGTGA ACGCCCTCGA
    TCAGAACAAG AGGAAGCACA GTTGTTAGAC GGCTTTTTAC GCTTTGTGGA GGGGCTAAAC
    CGAATCCGTC GACGCCACCG TTCTGATCGC ATGATCCGGA AAGGAGCAGG TATGAAGGGC
    CTGCAATTGC CTTCTCATCC GCCAGAACCT CCAGGTCCAC CTATAGGGAA GAAAGGAACA
    TCAGCACTAT CAGCATTGCT TGAGATGGAA GCTAATCAAA AGAGCCTGGG GGAGCAGCAA
    GCCCTTGTAC ATGCTGCAAA ATTGTCATCT CAGGTCTCAG ACCCTGCGGT AACTCCCACT
    TATGTGGACA GTCCTCGTAA GATCTCCATT GACCAAGGAA CTTCTTTAGT GACTGATGGA
    AATAATGCCA TGCCTAACCC TACCCCTTCT GAAAGAATAC AGAGTCAGGG TGCAACCTCT
    GTAGATCAGC CCCCCACTTC AACTGAATGC AGTGCACAGC AGATATCTGT GGGTCCAAGT
    GTATTACCAA CCACTGCCAG TCTGAGTGAG ATTCTGGAGG CTATGAAACA TCCAACTACA
    GGCATCCCTC TCCTGTCAGA GCAGAAGTGC CTTCCTCCTG GTTGCTTTAT CAGTGCTGAG
    GCTGTGCATT GGCTGGTCAA TAATATTAGC TGGGTGCAAA GTCAGGTTAT GGCCATGGAT
    ATCATGCAGA AGATGCTTGA AGAGCAGTAT ATCACACATG CGTCAGGAGA GACATTTAGA
    ACTTTCATCT ATGGCTTTTA CTTGTACAAG ATCCCAGCTG ACAAGGAGCC TGACAGAGCA
    TCAAACCTGG TACAGCCATG CGTGTGGCAT TCCACAGCCA TGGAGGATTT CACAGCCTTC
    CAACGGAAGT GGTTTGAGGT TGCATTGGTG GAGGAAGAGC TACTGCACTC CGAGATCCCA
    CCTTTTCTTT TGCCGTGGTT ACCTAGTCGC CCCGCATCTT ATGCAAGTAG GCACAGCTCC
    TTTAGCCGCA GCTTCGGAGG AAGGAGCCAG GCCGCTGCTC TGCTAGCTGC TACAGTGCCA
    GAGCAGAGAA CGGTCACTTT AGATGTGGAT GTTAATAACC GCACTGACCG GCTAGAATGG
    TGTAGCAGCT ACTATCACGG AAACTTCTCC TTGACTGCTG CTTTTGAGAT CAAACTGCAC
    TGGATGGCAG CAACTGCAGC AATGCTATTT GAGATGGTTC AAGGATGGCA CAGAAAAGCT
    CCGTCCTGCG GTTTCCTCCT AGTACCTGTT GTAGAAGGCC CTTTTTCTCT CCCTAGTTAC
    TTGTATGGGG ATCCTTTGCG TGCTCAGCTT TTTATTCCAC TTAATATCAG CTGCTTAGTA
    AAAGCTGAGA ATGACCATCT TTTTGAAGGT TTTGAACCAG AAACTTACTG GGAACGAATG
    TACTTGCTAC AGGAAGTAAT AGCTTATAGA TTTGGCTTTA TTCAGGACAA ATATTCAGCA
    TCTGCATTTA ACTTCCCATC AGAGAATAAA CCACAGTATA TACATGTGAC AGGTACTGTA
    TTCTTACAGC TGCCTTACTC AAAGCGGAAA GGACAGCAGA GACGTAGGAG GAATTCGACC
    AGCTCTTCTA CTCAGAACCT TTTTGGAGAA GAACGCATAG GGTATAATTG GGCCTACAAT
    ACCATGCTGA CTAAAACGTG GCGCTCCATT GGTACAGGAG ATGAGAGGTT TGCTGACCGA
    CTGCTTAAAG ACTTTACTGA CTTCTGTGCC AACAGAGATA ACAGGTTAGG CACATTCTGG
    GCAAACTGCT TGGAGAAAAT GAACACAAGT GCGCCTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OXa37175
    Clone ID Related Accession (Same CDS sequence) XM_012969680.2
    Accession Version XM_012969680.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4815bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-09-07
    Organism Xenopus tropicalis(tropical clawed frog)
    Product DEP domain-containing protein 5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030677.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 8, 2016 this sequence version replaced XM_012969680.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGTG CCTGGTTTCT GCTGCACGGT TGGGGTGGAT
    TGGAAGTCCC TCACCACCCC AGCCTGCCTG CCGCTTACCA CAGACTACTT CCCAGATAGG
    CAAAGTCTGC AAAATGATTA CACAGAGGGA TGCTATGACC TTCTGCCAGA AGCTGACCTT
    GACAGACGAG ATGAAGAGGG AGCTCAGATG ACTGCACAAC AAGTTTTTGA AGAGTTTATC
    TGCCAACGTC TTATGCAAGG TTATCAAATT ATTGTTCAGC CAAAACACCC AAAAGCCATC
    TCCTCTGTTC CTCCTCCACT CAGCAGCAGC CCGCTTTACA GAAGAGGGCT GGTGTCAAGG
    AACAGAGCTG AAGAGGAGAA CCAGTACTGG CTGAGTATGG GCCGAACCTT CCATAAGGTG
    ACACTAAAGG ACAAGATAAT AACAGTCACT AGATATCTAC CCAAGTATCC ATATGAATCT
    GCCCAAATCA GCTACATGTA CAGTCTGTGC CCATCACACT CTGAGTGTGA TTTTGTGTCC
    AGCTGGGTGG AATTCTCCCA TGAGCGACTG GAGGATTATA AATGGAACTA TTTGGATCAG
    TATATATGCT CAGCTGGCTC TGAAGATTTC AGTCTCATCG AATCCCTAAA GTTTTGGCGC
    ACACGTTTCC TTTTGCTCCC TTCTTGTGTG GGACCCACAA AACGCATCAC TGAGGGGGAA
    CAACGCTGTG ATATATATGG TGAACGCCCT CGATCAGAAC AAGAGGAAGC ACAGTTGTTA
    GACGGCTTTT TACGCTTTGT GGAGGGGCTA AACCGAATCC GTCGACGCCA CCGTTCTGAT
    CGCATGATCC GGAAAGGAGC AGGTATGAAG GGCCTGCAAT TGCCTTCTCA TCCGCCAGAA
    CCTCCAGGTC CACCTATAGG GAAGAAAGGA ACATCAGCAC TATCAGCATT GCTTGAGATG
    GAAGCTAATC AAAAGAGCCT GGGGGAGCAG CAAGCCCTTG TACATGCTGC AAAATTGTCA
    TCTCAGGTCT CAGACCCTGC GGTAACTCCC ACTTATGTGG ACAGTCCTCG TAAGGATGGG
    GCCTTCTTTG TGGAGTTTGT ACGCAGTCCA CGCACAGCGC CTTCCTTCAC TCCTCAGATC
    TCCATTGACC AAGGAACTTC TTTAGTGACT GATGGAAATA ATGCCATGCC TAACCCTACC
    CCTTCTGAAA GAATACAGAG TCAGGGTGCA ACCTCTGTAG ATCAGCCCCC CACTTCAACT
    GAATGCAGTG CACAGCAGAT ATCTGTGGGT CCAAGTGTAT TACCAACCAC TGCCAGTCTG
    AGTGAGATTC TGGAGGCTAT GAAACATCCA ACTACAGGCA TCCCTCTCCT GTCAGAGCAG
    AAGTGCCTTC CTCCTGGTTG CTTTATCAGT GCTGAGGCTG TGCATTGGCT GGTCAATAAT
    ATTAGCTGGG TGCAAAGTCA GGTTATGGCC ATGGATATCA TGCAGAAGAT GCTTGAAGAG
    CAGTATATCA CACATGCGTC AGGAGAGACA TTTAGAACTT TCATCTATGG CTTTTACTTG
    TACAAGATCC CAGCTGACAA GGAGCCTGAC AGAGCATCAA ACCTGGTACA GCCATGCGTG
    TGGCATTCCA CAGCCATGGA GGATTTCACA GCCTTCCAAC GGAAGTGGTT TGAGGTTGCA
    TTGGTGGAGG AAGAGCTACT GCACTCCGAG ATCCCACCTT TTCTTTTGCC GTGGTTACCT
    AGTCGCCCCG CATCTTATGC AAGTAGGCAC AGCTCCTTTA GCCGCAGCTT CGGAGGAAGG
    AGCCAGGCCG CTGCTCTGCT AGCTGCTACA GTGCCAGAGC AGAGAACGGT CACTTTAGAT
    GTGGATGTTA ATAACCGCAC TGACCGGCTA GAATGGTGTA GCAGCTACTA TCACGGAAAC
    TTCTCCTTGA CTGCTGCTTT TGAGATCAAA CTGCACTGGA TGGCAGCAAC TGCAGCAATG
    CTATTTGAGA TGGTTCAAGG ATGGCACAGA AAAGCTCCGT CCTGCGGTTT CCTCCTAGTA
    CCTGTTGTAG AAGGCCCTTT TTCTCTCCCT AGTTACTTGT ATGGGGATCC TTTGCGTGCT
    CAGCTTTTTA TTCCACTTAA TATCAGCTGC TTAGTAAAAG CTGAGAATGA CCATCTTTTT
    GAAGGTTTTG AACCAGAAAC TTACTGGGAA CGAATGTACT TGCTACAGGA AGTAATAGCT
    TATAGATTTG GCTTTATTCA GGACAAATAT TCAGCATCTG CATTTAACTT CCCATCAGAG
    AATAAACCAC AGTATATACA TGTGACAGGT ACTGTATTCT TACAGCTGCC TTACTCAAAG
    CGGAAAGGAC AGCAGAGACG TAGGAGGAAT TCGACCAGCT CTTCTACTCA GAACCTTTTT
    GGAGAAGAAC GCATAGGGTA TAATTGGGCC TACAATACCA TGCTGACTAA AACGTGGCGC
    TCCATTGGTA CAGGAGATGA GAGGTTTGCT GACCGACTGC TTAAAGACTT TACTGACTTC
    TGTGCCAACA GAGATAACAG GTTAGGCACA TTCTGGGCAA ACTGCTTGGA GAAAATGAAC
    ACAAGTGCGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_012825134.1
    CDS48..4862
    Translation

    Target ORF information:

    RefSeq Version XM_012969680.2
    Organism Xenopus tropicalis(tropical clawed frog)
    Definition Xenopus tropicalis DEP domain containing 5 (depdc5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_012969680.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGTTGTTGG TTGTTATTTC CTGTATTGGA TGCATCACTT GCCCAGATGT GCTTCAAATC 
    AGCAGAGAAT CCAGCATGAG GACCAACAGA GTATACAAGC TAGTCATCCA CAAGAAAGGC
    TTTGGGGGCA GTGATGATGA GTTGGTGATA AACCCCAAGG TCTTTCCATA TATTAACCTG
    GGGGACATTG TTGAAATTGC TCACCCAAAT GATGAATACA GTCCCCTTCT CCTGCAGGTG
    AAGTCACTAA AAGAAGATTT GCAAAAAGAA ACTGTTAGTA TTGACCAAAC CGTAGCCCAA
    GTGTTTCGCC TACGGCCATA CCAGGATGTT TATGTCAATG TTGTTGACCC TAAGGATGTG
    ACTCTTGATC TTGTGGAGTT AACATTTAAG GACCAGTATA TAGGTCGTGG AGATATGTGG
    AGGCTAAAAA AGAGTTTGGT CAGCTCCTGT GCTTACATAA CTCAGAAAGT GGAGTTTGCT
    GGAATTCGAG CACAAGTTGG AGAATTATGG GTTAAGAGCG AGAAAGTCAC CTGTGGGTAC
    ATCAGTGAAG ACACACGAGT TGTATTCAGG TCCACATCGG CCATGGTGTA TATATTTATA
    CAAATGAGCA GTGAACTGTG GGACTTTGAT ATCTATGGTG ATCTGTATTT TGAGAAGGCA
    GTAAATGGAT TTCTGGCTGA TCTCTTTAAC AAATGGAAGG ACAAGAACTG CAGTCACGAG
    GTTACAGTGG TTCTATTCTC TAGGACCTTT TATGATGCAA AGAATATTGA GGACTTTCCA
    GAAGCACACC GTAATGCGTC CGTCATGCAA GATCACAAGG GCAGATTCTA TGAAGACTTC
    TACAAGGTGG TTGTACAAAA TGAAAGGCGA GAAGAATGGA CTTCATTGCT AGTCACTATT
    AAGAAACTTT TTATTCAGTA CCCAGTGCTT GTACGACTGG AAAATGCAGA GGGCTTTCCC
    CCAGGGCATA ATTCTACTTC AGATCAAGGA AATTATCTAG AGGCCATTAA CCTGTCCTTT
    AATGTGTTTG ACAAGCATTA TATAAATCGC AACTTTGACC GAACTGGTCA GATGTCTGTT
    GTGATCACTC CAGGTGTTGG TGTTTTCCAA GTGGATCGTT CACTAATGAT TCTGACCAAG
    CAGCGAATGA TTGATAATGG GATTGGTGTG GATCTGGTAT GTATGGGAGA GCAGCCGCTT
    CATGCTGTTC CACTTTTCAA GCTGCACAAT CGTTCCAGTC CAGGAGATTC ACGTCTTGGA
    GATGATTATA ACATCCCTCA CTGGATTAAC CATAGCTTCT ACACATCGCA GAGCCAGCCT
    CTAAATAACA GCTTTACCCC ACGCATCAAA CTGGCACCTC GCAAGACAAA TAGTGAGAAA
    GGAAGGACTG GAAAAGACCC ATCCATAGGA AGCCCCAAAG AGACAGAGAA TGCTCTTCCC
    ATACAAGTAG ACTACGATGC TTATGACTCA CAGGTCTTCC GATTACCCGG TCCATCCAGG
    GCACAGCGAA GCATAACTTT CAGGGCTTTT TGGGCATTTT GGAGATTTCT TCTTTTCCTC
    CAGTCGCTGC CTTGGCAAAC CATAGAAGAG GGAAAGAAGG CAGTGCGAGA GACACAAGTT
    CGAAAAAGTT CTTGTTCCTA TGACACATCC TGTAGCCCAG TCAGTCGGCC ATTTACAGCA
    GAGGAAGGCC GTAGCCAAAA TTCAGATGAG AGCTCTTTGG GAAAACTCTC CAACATCCTA
    CTTATCCCAA GGCCACATCT GCATCAATAT GAGGCCAGCA GCTCTTTGGG TTATACTAGC
    ACAAGAGATT TCTTGGAGAG GATGATTGAA TCCCAACAGC GAGACTCAAG TGCTCCTGGC
    AGATTCCATG TGGGCAGTGC AGAATCCATG TTACACGTCC GACCTGGGGG ATACCACCCG
    CAGAGAGCAC TCATCAACCC ATTTGCACCT TCACGTATGC CAATGAAACT GACATCAAAT
    CGCAGGAGGT GGATGCACAC ATTTCCAGTG GGCCCATCTG GAGAAGCAAT ACAAATACAT
    CACCAAACTC GTCAGAATAT GGCAGAGCTA CAGGGTAGTG GCATGCGTGA GGCATCTCAT
    TCTTCTGCAG AGTTGCTAGA ATTGGCTTAC CATGAAGCCA CTGGCAGACA CAGTGGCCCT
    CGCACCCCTG GGGAAAATGG CTCTTGTGGG AGCAATGAAG AATTCTCCTG CAACACACCC
    ATCAATGGGC AGAGGAACCA CAATACAGTG CCTGGTTTCT GCTGCACGGT TGGGGTGGAT
    TGGAAGTCCC TCACCACCCC AGCCTGCCTG CCGCTTACCA CAGACTACTT CCCAGATAGG
    CAAAGTCTGC AAAATGATTA CACAGAGGGA TGCTATGACC TTCTGCCAGA AGCTGACCTT
    GACAGACGAG ATGAAGAGGG AGCTCAGATG ACTGCACAAC AAGTTTTTGA AGAGTTTATC
    TGCCAACGTC TTATGCAAGG TTATCAAATT ATTGTTCAGC CAAAACACCC AAAAGCCATC
    TCCTCTGTTC CTCCTCCACT CAGCAGCAGC CCGCTTTACA GAAGAGGGCT GGTGTCAAGG
    AACAGAGCTG AAGAGGAGAA CCAGTACTGG CTGAGTATGG GCCGAACCTT CCATAAGGTG
    ACACTAAAGG ACAAGATAAT AACAGTCACT AGATATCTAC CCAAGTATCC ATATGAATCT
    GCCCAAATCA GCTACATGTA CAGTCTGTGC CCATCACACT CTGAGTGTGA TTTTGTGTCC
    AGCTGGGTGG AATTCTCCCA TGAGCGACTG GAGGATTATA AATGGAACTA TTTGGATCAG
    TATATATGCT CAGCTGGCTC TGAAGATTTC AGTCTCATCG AATCCCTAAA GTTTTGGCGC
    ACACGTTTCC TTTTGCTCCC TTCTTGTGTG GGACCCACAA AACGCATCAC TGAGGGGGAA
    CAACGCTGTG ATATATATGG TGAACGCCCT CGATCAGAAC AAGAGGAAGC ACAGTTGTTA
    GACGGCTTTT TACGCTTTGT GGAGGGGCTA AACCGAATCC GTCGACGCCA CCGTTCTGAT
    CGCATGATCC GGAAAGGAGC AGGTATGAAG GGCCTGCAAT TGCCTTCTCA TCCGCCAGAA
    CCTCCAGGTC CACCTATAGG GAAGAAAGGA ACATCAGCAC TATCAGCATT GCTTGAGATG
    GAAGCTAATC AAAAGAGCCT GGGGGAGCAG CAAGCCCTTG TACATGCTGC AAAATTGTCA
    TCTCAGGTCT CAGACCCTGC GGTAACTCCC ACTTATGTGG ACAGTCCTCG TAAGGATGGG
    GCCTTCTTTG TGGAGTTTGT ACGCAGTCCA CGCACAGCGC CTTCCTTCAC TCCTCAGATC
    TCCATTGACC AAGGAACTTC TTTAGTGACT GATGGAAATA ATGCCATGCC TAACCCTACC
    CCTTCTGAAA GAATACAGAG TCAGGGTGCA ACCTCTGTAG ATCAGCCCCC CACTTCAACT
    GAATGCAGTG CACAGCAGAT ATCTGTGGGT CCAAGTGTAT TACCAACCAC TGCCAGTCTG
    AGTGAGATTC TGGAGGCTAT GAAACATCCA ACTACAGGCA TCCCTCTCCT GTCAGAGCAG
    AAGTGCCTTC CTCCTGGTTG CTTTATCAGT GCTGAGGCTG TGCATTGGCT GGTCAATAAT
    ATTAGCTGGG TGCAAAGTCA GGTTATGGCC ATGGATATCA TGCAGAAGAT GCTTGAAGAG
    CAGTATATCA CACATGCGTC AGGAGAGACA TTTAGAACTT TCATCTATGG CTTTTACTTG
    TACAAGATCC CAGCTGACAA GGAGCCTGAC AGAGCATCAA ACCTGGTACA GCCATGCGTG
    TGGCATTCCA CAGCCATGGA GGATTTCACA GCCTTCCAAC GGAAGTGGTT TGAGGTTGCA
    TTGGTGGAGG AAGAGCTACT GCACTCCGAG ATCCCACCTT TTCTTTTGCC GTGGTTACCT
    AGTCGCCCCG CATCTTATGC AAGTAGGCAC AGCTCCTTTA GCCGCAGCTT CGGAGGAAGG
    AGCCAGGCCG CTGCTCTGCT AGCTGCTACA GTGCCAGAGC AGAGAACGGT CACTTTAGAT
    GTGGATGTTA ATAACCGCAC TGACCGGCTA GAATGGTGTA GCAGCTACTA TCACGGAAAC
    TTCTCCTTGA CTGCTGCTTT TGAGATCAAA CTGCACTGGA TGGCAGCAAC TGCAGCAATG
    CTATTTGAGA TGGTTCAAGG ATGGCACAGA AAAGCTCCGT CCTGCGGTTT CCTCCTAGTA
    CCTGTTGTAG AAGGCCCTTT TTCTCTCCCT AGTTACTTGT ATGGGGATCC TTTGCGTGCT
    CAGCTTTTTA TTCCACTTAA TATCAGCTGC TTAGTAAAAG CTGAGAATGA CCATCTTTTT
    GAAGGTTTTG AACCAGAAAC TTACTGGGAA CGAATGTACT TGCTACAGGA AGTAATAGCT
    TATAGATTTG GCTTTATTCA GGACAAATAT TCAGCATCTG CATTTAACTT CCCATCAGAG
    AATAAACCAC AGTATATACA TGTGACAGGT ACTGTATTCT TACAGCTGCC TTACTCAAAG
    CGGAAAGGAC AGCAGAGACG TAGGAGGAAT TCGACCAGCT CTTCTACTCA GAACCTTTTT
    GGAGAAGAAC GCATAGGGTA TAATTGGGCC TACAATACCA TGCTGACTAA AACGTGGCGC
    TCCATTGGTA CAGGAGATGA GAGGTTTGCT GACCGACTGC TTAAAGACTT TACTGACTTC
    TGTGCCAACA GAGATAACAG GTTAGGCACA TTCTGGGCAA ACTGCTTGGA GAAAATGAAC
    ACAAGTGCGC CTTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.