Iml1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Iml1
Entrez Gene ID	38176
Full Name	Increased minichromosome loss 1
Synonyms	CG12090,Dmel\CG12090,SEA1,iml1
General protein information	Preferred Names Increased minichromosome loss 1 Names CG12090 gene product from transcript CG12090-RB CG12090-PA CG12090-PB CG12090-PC CG12090-PD CG12090-PE Iml1-PA Iml1-PB Iml1-PC Iml1-PD Iml1-PE increased minichromosome loss 1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3L Map Location: 62A3-62A3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001274330.1	NP_001261259.1	increased minichromosome loss 1, isoform D
NM_001274331.1	NP_001261260.1	increased minichromosome loss 1, isoform E
NM_139361.2	NP_647618.1	increased minichromosome loss 1, isoform B
NM_167889.2	NP_728621.1	increased minichromosome loss 1, isoform A
NM_167888.2	NP_728620.1	increased minichromosome loss 1, isoform C

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DEPDC5	XP_415249.4
Mus musculus (house mouse)	Depdc5	NP_001164038.1
Rattus norvegicus (Norway rat)	Depdc5	NP_001100699.1
Homo sapiens (human)	DEPDC5	NP_001229825.1
Canis lupus familiaris (dog)	DEPDC5	XP_005636547.1
Bos taurus (cattle)	LOC521542	XP_005218248.1
Xenopus tropicalis (tropical clawed frog)	LOC100498286	XP_004910648.1
Pan troglodytes (chimpanzee)	DEPDC5	XP_003317262.1
Danio rerio (zebrafish)	depdc5	XP_005155706.1
Drosophila melanogaster (fruit fly)	CG12090	NP_001261260.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP007010	XP_308760.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Iml1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Iml1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa17167	NM_139361.2 Latest version!	Drosophila melanogaster increased minichromosome loss 1, transcript variant B (Iml1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa17168	NM_167888.2 Latest version!	Drosophila melanogaster increased minichromosome loss 1, transcript variant C (Iml1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
OFa17169	NM_167889.2 Latest version!	Drosophila melanogaster increased minichromosome loss 1, transcript variant A (Iml1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa20424	NM_001274330.1 Latest version!	Drosophila melanogaster increased minichromosome loss 1, transcript variant D (Iml1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
OFa20434	NM_001274331.1 Latest version!	Drosophila melanogaster increased minichromosome loss 1, transcript variant E (Iml1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Iml1 ( NM_139361.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_647618.1 Drosophila melanogaster increased minichromosome loss 1, transcript variant B (Iml1), mRNA.

Price & Availability↑

CloneID	OFa17167
Clone ID Related Accession (Same CDS sequence)	NM_139361.2
Accession Version	NM_139361.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	increased minichromosome loss 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_139361.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGGCG TCGATTGGAA GTCGCTTACG ATCCCCGCCT GTCTGCCCAT CACCACGGAC 
TACTTCCCGG ACAAGCGTTC GCTGAATAAC GACTATGTGA TATCGGATTA CACTCTGCTG 
CCCGACGATG TGAATCACGA TTACGCCCAG AGCAGAGCGG TTTACCGGAA ACCCCTCTCC 
ACGGAGGAGG TGTGCAAGGA GATCGTGTCG CAGCGCTTGG CGCAGGGCTT CCAGCTAATC 
GTGGTCGACG AGAAGCCTCC GACTGCGAGC GGTTGTTCCT CGGGATCTGC AGTGCAGCCG 
GTGAAGCTTT CGCGCGAGAC CAACAAGGAG TATCTGCTCT CCATTGGCCG CATCTTTCAC 
AAGATCTCGT TGAGCGGCTC GGTGATCACG GTCACTGGCT ATAGGCCCAG ACACCCGTAT 
CCTCCGATAA ATGTGGACTA CCGCTACCGT TTCCATGCCC CACAACACGA GACCTACGAA 
ATATCCGGCG TGAACTTCAC CACCGAGAAG TTGGAGAACT TCAACTGGAA CCACATGGAC 
CTGTACATCT GCACGCGTGG CGATGTGGAT TACCCACTCA TGGAGAGCCT GAAGTACTGG 
CGCTATCGCA TGTACCTGCT GCCCAGGGAG AACATTGTGA GCAAGATCGC CAGTTGCCAG 
CGATGCGACA TATTCCCCGA CGTGACTGCG GATAACACGC GGGAGCAGGT CGAGGACTTC 
GTCCGACTGA TCGAGGCGGT CAGCAAGCTG AAGCGGCAAT ATGCGCGCAA GGCGAGACAG 
CATGACACAC CTAGAATCAC TGAAAAGCAT CATAACCAAT TAAACTCACC GCTGCAAAGC 
ATCAATGTGC GCCCCAAACT GGAGAACGGT CGAATTCCAC GAATATTTCC CGCCACCGAT 
GCAGCGGCAG CTGCAGGAGT CGCCGCCAGA GATGATCAGG ACGATGGTTT CCCAGTCGAC 
ATTAAGTTCA GCCCGAATGC CACGCTGCCC GAAATCTTCG AGGCCATGAA GCACCCGGTA 
AACGGAGTGG GCTTCTTCTC GCAGACGCAG TCGCTCCCCT CCTGCACCTT TGTGTCCTAC 
GACGCACTAA TGTGGTTGAA AACCCGCCTG AACAATGGAC GACATCCCCT GGATCTACTG 
GAGGCCATGC GCAAAGAGCG CATGATCTGC CATGCTTCGG GGGATTGGAA GAAGCCCGTG 
GTGCCTGGCT TTGTTTTCTA CTATGTGGTG CAGCAGGATA AAAACGCCAA AGATTACGCC 
CCGCCTTTGG ATGACTACAG TGCCTTTGTG AACGAGTGGT TGGAGATTGA GTTTCAGGGT 
TGCAGTTTTC TCTGGCACGA TGAGCCAGTG ACCACTCCAG TGCCCAATTT CCTTAGGGAC 
TCACCTGCTC CGCAATCCTG GACAGAAACG AGTAGCAACA AGCGCGTATA TCGACAGTCG 
CATTTGGAGA TCGATGTGAA CCAGAAGAGC GATCGCATGG AGTGGGGCCA TGTGAAGCAT 
CACACAGTGC TGCAGCCGAG ATTCGCCTTT GAGATCGTGG TTGAGTGGGT CACCTCATCG 
GGTCCCATAG TGGCTGACTT GATTGGCGGC TGGATGCGCA AGGCGAATCA GTTCAATTTC 
CTGGTATCAG TGCCAGCGGA TCCAATGGCA GAGCCGTTCA CCAAGAAGTC GGATCCTCTG 
CGGGGACCCA TTTTCATTCC GCTTTGCACT ACATTCCTGC CCAATGGCGC TGCCCTCTTC 
GATGAGTTTC CCGAGGAGAG CAGATCAGAC AGAATGCTGT TCTTCCAGGA AGCCATTTTG 
GGCAAGTTTG GCTTCTTGCC CTGCGTTTTG GAGAAGAAGT ACAGCGTTGG CAAGGATCTG 
CCCAAGGAGT ACCAGTATGT GCACTGCACG GGCAACATGT TTGCCTTGAT CCGTTGCGCT 
ACAAACAACT ACCAGGTGGA ATCGCCCATC CTGAAGGAGG CAAATGTCAC GCGCTGCGTC 
TATGGGCACA CCAACAACAC GAACGTGCCC AAGAAAGTGG GTTTCTTGTG GGCGTGGAAC 
CACATGATTC CGAACAAGAA ATGGAAGGCC CAGACGATAA ACAACTCCGC GGACGGGGAG 
CTCTTCCAGC TGAAGATGCT CAAGGATTTC CGCGAGTTTT GCTCGAACAG CGATCAGCGC 
CTGTCCACTT TCTGGACCCA ATCGCAGGAG TTGAAGCGAA GGGCCCAGAA ATTCGAGTTT 
AACAATAACA ACACTGAGGA GAATAAGATG AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_647618.1
CDS	127..4542
Translation	MKLYKLNTHTRGCNKSYDADLVMNLKDHPNANVGDVVEIYAPDEENGTHLLLQITEFNGSCGRDVISIESGIANAFKMRPYSNVVMRIVKPADVALDSIEITFKDQYMGRSEMWRLKTYLTDTCVYVNKKIDYNDMQIRCQVYEMWSQGERVASGVITEDTKIVFRSSTSMVYLFLQMSSEMWDFDIHGDLYFEKAVNGFLTELFQKWRKLSCNHEVTIVLFSRTFYAAKSLDEFPEHMRDCLQQDYKGRFYEDFYRVAIQNERCDDWCTVLGQLRKLFTSYQATVLRYHERENMKIPPATNSSATQGNFLEVLNISLNTFEKHYLDRTFDRTGQLSVVITPGVGVFSVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDTTLTSADDYSLPHWINLSFYSTNKKIVYSSFIPRIKLPLFGSQLTLHDGVGDGEGEENERHFLSCNQSEYKHNSLFDYDAYDEQIFQPLPAQSTCSLQRVVRAKKTSVPSLETYAYRNNDWENLTPTQIPAMRRKMSDPDIHHGTSAMLAALQPDTTNLSESLASEKNSRRTIVSIAPIVRPGRALINPFDPSQVTIKLTSNRRRWTHIFPKGPTGVLIQQHHYQAVPAKPTQAGQQRPLQQTQSNNNNDQEDYGCENGEQYDRVSSHSLLNKSDSSQSFVMGDEPIDFFKRRQNSPMNPQPANVPNLTATQAKSYLWGATGEQEWTPAITTGVDWKSLTIPACLPITTDYFPDKRSLNNDYVISDYTLLPDDVNHDYAQSRAVYRKPLSTEEVCKEIVSQRLAQGFQLIVVDEKPPTASGCSSGSAVQPVKLSRETNKEYLLSIGRIFHKISLSGSVITVTGYRPRHPYPPINVDYRYRFHAPQHETYEISGVNFTTEKLENFNWNHMDLYICTRGDVDYPLMESLKYWRYRMYLLPRENIVSKIASCQRCDIFPDVTADNTREQVEDFVRLIEAVSKLKRQYARKARQHDTPRITEKHHNQLNSPLQSINVRPKLENGRIPRIFPATDAAAAAGVAARDDQDDGFPVDIKFSPNATLPEIFEAMKHPVNGVGFFSQTQSLPSCTFVSYDALMWLKTRLNNGRHPLDLLEAMRKERMICHASGDWKKPVVPGFVFYYVVQQDKNAKDYAPPLDDYSAFVNEWLEIEFQGCSFLWHDEPVTTPVPNFLRDSPAPQSWTETSSNKRVYRQSHLEIDVNQKSDRMEWGHVKHHTVLQPRFAFEIVVEWVTSSGPIVADLIGGWMRKANQFNFLVSVPADPMAEPFTKKSDPLRGPIFIPLCTTFLPNGAALFDEFPEESRSDRMLFFQEAILGKFGFLPCVLEKKYSVGKDLPKEYQYVHCTGNMFALIRCATNNYQVESPILKEANVTRCVYGHTNNTNVPKKVGFLWAWNHMIPNKKWKAQTINNSADGELFQLKMLKDFREFCSNSDQRLSTFWTQSQELKRRAQKFEFNNNNTEENKMK

Target ORF information:

RefSeq Version	NM_139361.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster increased minichromosome loss 1, transcript variant B (Iml1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_139361.2

ORF Insert Sequence:

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGGCG TCGATTGGAA GTCGCTTACG ATCCCCGCCT GTCTGCCCAT CACCACGGAC 
TACTTCCCGG ACAAGCGTTC GCTGAATAAC GACTATGTGA TATCGGATTA CACTCTGCTG 
CCCGACGATG TGAATCACGA TTACGCCCAG AGCAGAGCGG TTTACCGGAA ACCCCTCTCC 
ACGGAGGAGG TGTGCAAGGA GATCGTGTCG CAGCGCTTGG CGCAGGGCTT CCAGCTAATC 
GTGGTCGACG AGAAGCCTCC GACTGCGAGC GGTTGTTCCT CGGGATCTGC AGTGCAGCCG 
GTGAAGCTTT CGCGCGAGAC CAACAAGGAG TATCTGCTCT CCATTGGCCG CATCTTTCAC 
AAGATCTCGT TGAGCGGCTC GGTGATCACG GTCACTGGCT ATAGGCCCAG ACACCCGTAT 
CCTCCGATAA ATGTGGACTA CCGCTACCGT TTCCATGCCC CACAACACGA GACCTACGAA 
ATATCCGGCG TGAACTTCAC CACCGAGAAG TTGGAGAACT TCAACTGGAA CCACATGGAC 
CTGTACATCT GCACGCGTGG CGATGTGGAT TACCCACTCA TGGAGAGCCT GAAGTACTGG 
CGCTATCGCA TGTACCTGCT GCCCAGGGAG AACATTGTGA GCAAGATCGC CAGTTGCCAG 
CGATGCGACA TATTCCCCGA CGTGACTGCG GATAACACGC GGGAGCAGGT CGAGGACTTC 
GTCCGACTGA TCGAGGCGGT CAGCAAGCTG AAGCGGCAAT ATGCGCGCAA GGCGAGACAG 
CATGACACAC CTAGAATCAC TGAAAAGCAT CATAACCAAT TAAACTCACC GCTGCAAAGC 
ATCAATGTGC GCCCCAAACT GGAGAACGGT CGAATTCCAC GAATATTTCC CGCCACCGAT 
GCAGCGGCAG CTGCAGGAGT CGCCGCCAGA GATGATCAGG ACGATGGTTT CCCAGTCGAC 
ATTAAGTTCA GCCCGAATGC CACGCTGCCC GAAATCTTCG AGGCCATGAA GCACCCGGTA 
AACGGAGTGG GCTTCTTCTC GCAGACGCAG TCGCTCCCCT CCTGCACCTT TGTGTCCTAC 
GACGCACTAA TGTGGTTGAA AACCCGCCTG AACAATGGAC GACATCCCCT GGATCTACTG 
GAGGCCATGC GCAAAGAGCG CATGATCTGC CATGCTTCGG GGGATTGGAA GAAGCCCGTG 
GTGCCTGGCT TTGTTTTCTA CTATGTGGTG CAGCAGGATA AAAACGCCAA AGATTACGCC 
CCGCCTTTGG ATGACTACAG TGCCTTTGTG AACGAGTGGT TGGAGATTGA GTTTCAGGGT 
TGCAGTTTTC TCTGGCACGA TGAGCCAGTG ACCACTCCAG TGCCCAATTT CCTTAGGGAC 
TCACCTGCTC CGCAATCCTG GACAGAAACG AGTAGCAACA AGCGCGTATA TCGACAGTCG 
CATTTGGAGA TCGATGTGAA CCAGAAGAGC GATCGCATGG AGTGGGGCCA TGTGAAGCAT 
CACACAGTGC TGCAGCCGAG ATTCGCCTTT GAGATCGTGG TTGAGTGGGT CACCTCATCG 
GGTCCCATAG TGGCTGACTT GATTGGCGGC TGGATGCGCA AGGCGAATCA GTTCAATTTC 
CTGGTATCAG TGCCAGCGGA TCCAATGGCA GAGCCGTTCA CCAAGAAGTC GGATCCTCTG 
CGGGGACCCA TTTTCATTCC GCTTTGCACT ACATTCCTGC CCAATGGCGC TGCCCTCTTC 
GATGAGTTTC CCGAGGAGAG CAGATCAGAC AGAATGCTGT TCTTCCAGGA AGCCATTTTG 
GGCAAGTTTG GCTTCTTGCC CTGCGTTTTG GAGAAGAAGT ACAGCGTTGG CAAGGATCTG 
CCCAAGGAGT ACCAGTATGT GCACTGCACG GGCAACATGT TTGCCTTGAT CCGTTGCGCT 
ACAAACAACT ACCAGGTGGA ATCGCCCATC CTGAAGGAGG CAAATGTCAC GCGCTGCGTC 
TATGGGCACA CCAACAACAC GAACGTGCCC AAGAAAGTGG GTTTCTTGTG GGCGTGGAAC 
CACATGATTC CGAACAAGAA ATGGAAGGCC CAGACGATAA ACAACTCCGC GGACGGGGAG 
CTCTTCCAGC TGAAGATGCT CAAGGATTTC CGCGAGTTTT GCTCGAACAG CGATCAGCGC 
CTGTCCACTT TCTGGACCCA ATCGCAGGAG TTGAAGCGAA GGGCCCAGAA ATTCGAGTTT 
AACAATAACA ACACTGAGGA GAATAAGATG AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iml1 ( NM_167888.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728620.1 Drosophila melanogaster increased minichromosome loss 1, transcript variant C (Iml1), mRNA.

Price & Availability↑

CloneID	OFa17168
Clone ID Related Accession (Same CDS sequence)	NM_167888.2
Accession Version	NM_167888.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4419bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	increased minichromosome loss 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167888.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGGCG TCGATTGGAA GTCGCTTACG ATCCCCGCCT GTCTGCCCAT CACCACGGAC 
TACTTCCCGG ACAAGCGTTC GCTGAATAAC GACTATGTGA TATCGGATTA CACTCTGCTG 
CCCGACGATG TGAATCACGA TTACGCCCAG AGCAGAGCGG TTTACCGGAA ACCCCTCTCC 
ACGGAGGAGG TGTGCAAGGA GATCGTGTCG CAGCGCTTGG CGCAGGGCTT CCAGCTAATC 
GTGGTCGACG AGAAGCCTCC GACTGCGAGC GGTTGTTCCT CGGGATCTGC AGTGCAGCCG 
GTGAAGCTTT CGCGCGAGAC CAACAAGGAG TATCTGCTCT CCATTGGCCG CATCTTTCAC 
AAGATCTCGT TGAGCGGCTC GGTGATCACG GTCACTGGCT ATAGGCCCAG ACACCCGTAT 
CCTCCGATAA ATGTGGACTA CCGCTACCGT TTCCATGCCC CACAACACGA GACCTACGAA 
ATATCCGGCG TGAACTTCAC CACCGAGAAG TTGGAGAACT TCAACTGGAA CCACATGGAC 
CTGTACATCT GCACGCGTGG CGATGTGGAT TACCCACTCA TGGAGAGCCT GAAGTACTGG 
CGCTATCGCA TGTACCTGCT GCCCAGGGAG AACATTGTGA GCAAGATCGC CAGTTGCCAG 
CGATGCGACA TATTCCCCGA CGTGACTGCG GATAACACGC GGGAGCAGGT CGAGGACTTC 
GTCCGACTGA TCGAGGCGGT CAGCAAGCTG AAGCGGCAAT ATGCGCGCAA GGCGAGAGGA 
CTCACGAACT CTCCGTTCCG GGAGCGAGTC GGCAGCAATC GACTGCCGGA GAAGCGACCC 
AGCATCAATG TGCGCCCCAA ACTGGAGAAC GGTCGAATTC CACGAATATT TCCCGCCACC 
GATGCAGCGG CAGCTGCAGG AGTCGCCGCC AGAGATGATC AGGACGATGG TTTCCCAGTC 
GACATTAAGT TCAGCCCGAA TGCCACGCTG CCCGAAATCT TCGAGGCCAT GAAGCACCCG 
GTAAACGGAG TGGGCTTCTT CTCGCAGACG CAGTCGCTCC CCTCCTGCAC CTTTGTGTCC 
TACGACGCAC TAATGTGGTT GAAAACCCGC CTGAACAATG GACGACATCC CCTGGATCTA 
CTGGAGGCCA TGCGCAAAGA GCGCATGATC TGCCATGCTT CGGGGGATTG GAAGAAGCCC 
GTGGTGCCTG GCTTTGTTTT CTACTATGTG GTGCAGCAGG ATAAAAACGC CAAAGATTAC 
GCCCCGCCTT TGGATGACTA CAGTGCCTTT GTGAACGAGT GGTTGGAGAT TGAGTTTCAG 
GGTTGCAGTT TTCTCTGGCA CGATGAGCCA GTGACCACTC CAGTGCCCAA TTTCCTTAGG 
GACTCACCTG CTCCGCAATC CTGGACAGAA ACGAGTAGCA ACAAGCGCGT ATATCGACAG 
TCGCATTTGG AGATCGATGT GAACCAGAAG AGCGATCGCA TGGAGTGGGG CCATGTGAAG 
CATCACACAG TGCTGCAGCC GAGATTCGCC TTTGAGATCG TGGTTGAGTG GGTCACCTCA 
TCGGGTCCCA TAGTGGCTGA CTTGATTGGC GGCTGGATGC GCAAGGCGAA TCAGTTCAAT 
TTCCTGGTAT CAGTGCCAGC GGATCCAATG GCAGAGCCGT TCACCAAGAA GTCGGATCCT 
CTGCGGGGAC CCATTTTCAT TCCGCTTTGC ACTACATTCC TGCCCAATGG CGCTGCCCTC 
TTCGATGAGT TTCCCGAGGA GAGCAGATCA GACAGAATGC TGTTCTTCCA GGAAGCCATT 
TTGGGCAAGT TTGGCTTCTT GCCCTGCGTT TTGGAGAAGA AGTACAGCGT TGGCAAGGAT 
CTGCCCAAGG AGTACCAGTA TGTGCACTGC ACGGGCAACA TGTTTGCCTT GATCCGTTGC 
GCTACAAACA ACTACCAGGT GGAATCGCCC ATCCTGAAGG AGGCAAATGT CACGCGCTGC 
GTCTATGGGC ACACCAACAA CACGAACGTG CCCAAGAAAG TGGGTTTCTT GTGGGCGTGG 
AACCACATGA TTCCGAACAA GAAATGGAAG GCCCAGACGA TAAACAACTC CGCGGACGGG 
GAGCTCTTCC AGCTGAAGAT GCTCAAGGAT TTCCGCGAGT TTTGCTCGAA CAGCGATCAG 
CGCCTGTCCA CTTTCTGGAC CCAATCGCAG GAGTTGAAGC GAAGGGCCCA GAAATTCGAG 
TTTAACAATA ACAACACTGA GGAGAATAAG ATGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728620.1
CDS	127..4545
Translation	MKLYKLNTHTRGCNKSYDADLVMNLKDHPNANVGDVVEIYAPDEENGTHLLLQITEFNGSCGRDVISIESGIANAFKMRPYSNVVMRIVKPADVALDSIEITFKDQYMGRSEMWRLKTYLTDTCVYVNKKIDYNDMQIRCQVYEMWSQGERVASGVITEDTKIVFRSSTSMVYLFLQMSSEMWDFDIHGDLYFEKAVNGFLTELFQKWRKLSCNHEVTIVLFSRTFYAAKSLDEFPEHMRDCLQQDYKGRFYEDFYRVAIQNERCDDWCTVLGQLRKLFTSYQATVLRYHERENMKIPPATNSSATQGNFLEVLNISLNTFEKHYLDRTFDRTGQLSVVITPGVGVFSVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDTTLTSADDYSLPHWINLSFYSTNKKIVYSSFIPRIKLPLFGSQLTLHDGVGDGEGEENERHFLSCNQSEYKHNSLFDYDAYDEQIFQPLPAQSTCSLQRVVRAKKTSVPSLETYAYRNNDWENLTPTQIPAMRRKMSDPDIHHGTSAMLAALQPDTTNLSESLASEKNSRRTIVSIAPIVRPGRALINPFDPSQVTIKLTSNRRRWTHIFPKGPTGVLIQQHHYQAVPAKPTQAGQQRPLQQTQSNNNNDQEDYGCENGEQYDRVSSHSLLNKSDSSQSFVMGDEPIDFFKRRQNSPMNPQPANVPNLTATQAKSYLWGATGEQEWTPAITTGVDWKSLTIPACLPITTDYFPDKRSLNNDYVISDYTLLPDDVNHDYAQSRAVYRKPLSTEEVCKEIVSQRLAQGFQLIVVDEKPPTASGCSSGSAVQPVKLSRETNKEYLLSIGRIFHKISLSGSVITVTGYRPRHPYPPINVDYRYRFHAPQHETYEISGVNFTTEKLENFNWNHMDLYICTRGDVDYPLMESLKYWRYRMYLLPRENIVSKIASCQRCDIFPDVTADNTREQVEDFVRLIEAVSKLKRQYARKARGLTNSPFRERVGSNRLPEKRPSINVRPKLENGRIPRIFPATDAAAAAGVAARDDQDDGFPVDIKFSPNATLPEIFEAMKHPVNGVGFFSQTQSLPSCTFVSYDALMWLKTRLNNGRHPLDLLEAMRKERMICHASGDWKKPVVPGFVFYYVVQQDKNAKDYAPPLDDYSAFVNEWLEIEFQGCSFLWHDEPVTTPVPNFLRDSPAPQSWTETSSNKRVYRQSHLEIDVNQKSDRMEWGHVKHHTVLQPRFAFEIVVEWVTSSGPIVADLIGGWMRKANQFNFLVSVPADPMAEPFTKKSDPLRGPIFIPLCTTFLPNGAALFDEFPEESRSDRMLFFQEAILGKFGFLPCVLEKKYSVGKDLPKEYQYVHCTGNMFALIRCATNNYQVESPILKEANVTRCVYGHTNNTNVPKKVGFLWAWNHMIPNKKWKAQTINNSADGELFQLKMLKDFREFCSNSDQRLSTFWTQSQELKRRAQKFEFNNNNTEENKMK

Target ORF information:

RefSeq Version	NM_167888.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster increased minichromosome loss 1, transcript variant C (Iml1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167888.2

ORF Insert Sequence:

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGGCG TCGATTGGAA GTCGCTTACG ATCCCCGCCT GTCTGCCCAT CACCACGGAC 
TACTTCCCGG ACAAGCGTTC GCTGAATAAC GACTATGTGA TATCGGATTA CACTCTGCTG 
CCCGACGATG TGAATCACGA TTACGCCCAG AGCAGAGCGG TTTACCGGAA ACCCCTCTCC 
ACGGAGGAGG TGTGCAAGGA GATCGTGTCG CAGCGCTTGG CGCAGGGCTT CCAGCTAATC 
GTGGTCGACG AGAAGCCTCC GACTGCGAGC GGTTGTTCCT CGGGATCTGC AGTGCAGCCG 
GTGAAGCTTT CGCGCGAGAC CAACAAGGAG TATCTGCTCT CCATTGGCCG CATCTTTCAC 
AAGATCTCGT TGAGCGGCTC GGTGATCACG GTCACTGGCT ATAGGCCCAG ACACCCGTAT 
CCTCCGATAA ATGTGGACTA CCGCTACCGT TTCCATGCCC CACAACACGA GACCTACGAA 
ATATCCGGCG TGAACTTCAC CACCGAGAAG TTGGAGAACT TCAACTGGAA CCACATGGAC 
CTGTACATCT GCACGCGTGG CGATGTGGAT TACCCACTCA TGGAGAGCCT GAAGTACTGG 
CGCTATCGCA TGTACCTGCT GCCCAGGGAG AACATTGTGA GCAAGATCGC CAGTTGCCAG 
CGATGCGACA TATTCCCCGA CGTGACTGCG GATAACACGC GGGAGCAGGT CGAGGACTTC 
GTCCGACTGA TCGAGGCGGT CAGCAAGCTG AAGCGGCAAT ATGCGCGCAA GGCGAGAGGA 
CTCACGAACT CTCCGTTCCG GGAGCGAGTC GGCAGCAATC GACTGCCGGA GAAGCGACCC 
AGCATCAATG TGCGCCCCAA ACTGGAGAAC GGTCGAATTC CACGAATATT TCCCGCCACC 
GATGCAGCGG CAGCTGCAGG AGTCGCCGCC AGAGATGATC AGGACGATGG TTTCCCAGTC 
GACATTAAGT TCAGCCCGAA TGCCACGCTG CCCGAAATCT TCGAGGCCAT GAAGCACCCG 
GTAAACGGAG TGGGCTTCTT CTCGCAGACG CAGTCGCTCC CCTCCTGCAC CTTTGTGTCC 
TACGACGCAC TAATGTGGTT GAAAACCCGC CTGAACAATG GACGACATCC CCTGGATCTA 
CTGGAGGCCA TGCGCAAAGA GCGCATGATC TGCCATGCTT CGGGGGATTG GAAGAAGCCC 
GTGGTGCCTG GCTTTGTTTT CTACTATGTG GTGCAGCAGG ATAAAAACGC CAAAGATTAC 
GCCCCGCCTT TGGATGACTA CAGTGCCTTT GTGAACGAGT GGTTGGAGAT TGAGTTTCAG 
GGTTGCAGTT TTCTCTGGCA CGATGAGCCA GTGACCACTC CAGTGCCCAA TTTCCTTAGG 
GACTCACCTG CTCCGCAATC CTGGACAGAA ACGAGTAGCA ACAAGCGCGT ATATCGACAG 
TCGCATTTGG AGATCGATGT GAACCAGAAG AGCGATCGCA TGGAGTGGGG CCATGTGAAG 
CATCACACAG TGCTGCAGCC GAGATTCGCC TTTGAGATCG TGGTTGAGTG GGTCACCTCA 
TCGGGTCCCA TAGTGGCTGA CTTGATTGGC GGCTGGATGC GCAAGGCGAA TCAGTTCAAT 
TTCCTGGTAT CAGTGCCAGC GGATCCAATG GCAGAGCCGT TCACCAAGAA GTCGGATCCT 
CTGCGGGGAC CCATTTTCAT TCCGCTTTGC ACTACATTCC TGCCCAATGG CGCTGCCCTC 
TTCGATGAGT TTCCCGAGGA GAGCAGATCA GACAGAATGC TGTTCTTCCA GGAAGCCATT 
TTGGGCAAGT TTGGCTTCTT GCCCTGCGTT TTGGAGAAGA AGTACAGCGT TGGCAAGGAT 
CTGCCCAAGG AGTACCAGTA TGTGCACTGC ACGGGCAACA TGTTTGCCTT GATCCGTTGC 
GCTACAAACA ACTACCAGGT GGAATCGCCC ATCCTGAAGG AGGCAAATGT CACGCGCTGC 
GTCTATGGGC ACACCAACAA CACGAACGTG CCCAAGAAAG TGGGTTTCTT GTGGGCGTGG 
AACCACATGA TTCCGAACAA GAAATGGAAG GCCCAGACGA TAAACAACTC CGCGGACGGG 
GAGCTCTTCC AGCTGAAGAT GCTCAAGGAT TTCCGCGAGT TTTGCTCGAA CAGCGATCAG 
CGCCTGTCCA CTTTCTGGAC CCAATCGCAG GAGTTGAAGC GAAGGGCCCA GAAATTCGAG 
TTTAACAATA ACAACACTGA GGAGAATAAG ATGAAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iml1 ( NM_167889.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_728621.1 Drosophila melanogaster increased minichromosome loss 1, transcript variant A (Iml1), mRNA.

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CloneID	OFa17169
Clone ID Related Accession (Same CDS sequence)	NM_167889.2
Accession Version	NM_167889.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	increased minichromosome loss 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). On Jan 16, 2013 this sequence version replaced NM_167889.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGTCA AGCATCTGAG GCCGATCGTC GAGGGCGAGC ATCATCATCT GGGCAGCCTG 
GAGGCACTAA GGGCTCTAGA CCCGCCCCCC GATGCGGAGG CGGGCGGCGG AAGAGGAAAG 
ATCATCATAG GCGTCGATTG GAAGTCGCTT ACGATCCCCG CCTGTCTGCC CATCACCACG 
GACTACTTCC CGGACAAGCG TTCGCTGAAT AACGACTATG TGATATCGGA TTACACTCTG 
CTGCCCGACG ATGTGAATCA CGATTACGCC CAGAGCAGAG CGGTTTACCG GAAACCCCTC 
TCCACGGAGG AGGTGTGCAA GGAGATCGTG TCGCAGCGCT TGGCGCAGGG CTTCCAGCTA 
ATCGTGGTCG ACGAGAAGCC TCCGACTGCG AGCGGTTGTT CCTCGGGATC TGCAGTGCAG 
CCGGTGAAGC TTTCGCGCGA GACCAACAAG GAGTATCTGC TCTCCATTGG CCGCATCTTT 
CACAAGATCT CGTTGAGCGG CTCGGTGATC ACGGTCACTG GCTATAGGCC CAGACACCCG 
TATCCTCCGA TAAATGTGGA CTACCGCTAC CGTTTCCATG CCCCACAACA CGAGACCTAC 
GAAATATCCG GCGTGAACTT CACCACCGAG AAGTTGGAGA ACTTCAACTG GAACCACATG 
GACCTGTACA TCTGCACGCG TGGCGATGTG GATTACCCAC TCATGGAGAG CCTGAAGTAC 
TGGCGCTATC GCATGTACCT GCTGCCCAGG GAGAACATTG TGAGCAAGAT CGCCAGTTGC 
CAGCGATGCG ACATATTCCC CGACGTGACT GCGGATAACA CGCGGGAGCA GGTCGAGGAC 
TTCGTCCGAC TGATCGAGGC GGTCAGCAAG CTGAAGCGGC AATATGCGCG CAAGGCGAGA 
TATCTCGCCT CACTCTTGCA GGACAGCCCC ATCGCCCACA TAACCAAGCG GAGACACAGC 
ACCAGCATTA TATCCAGGCC TCAGCCTAAC CAGGGACTCA CGAACTCTCC GTTCCGGGAG 
CGAGTCGGCA GCAATCGACT GCCGGAGAAG CGACCCAGCA TCAATGTGCG CCCCAAACTG 
GAGAACGGTC GAATTCCACG AATATTTCCC GCCACCGATG CAGCGGCAGC TGCAGGAGTC 
GCCGCCAGAG ATGATCAGGA CGATGGTTTC CCAGTCGACA TTAAGTTCAG CCCGAATGCC 
ACGCTGCCCG AAATCTTCGA GGCCATGAAG CACCCGGTAA ACGGAGTGGG CTTCTTCTCG 
CAGACGCAGT CGCTCCCCTC CTGCACCTTT GTGTCCTACG ACGCACTAAT GTGGTTGAAA 
ACCCGCCTGA ACAATGGACG ACATCCCCTG GATCTACTGG AGGCCATGCG CAAAGAGCGC 
ATGATCTGCC ATGCTTCGGG GGATTGGAAG AAGCCCGTGG TGCCTGGCTT TGTTTTCTAC 
TATGTGGTGC AGCAGGATAA AAACGCCAAA GATTACGCCC CGCCTTTGGA TGACTACAGT 
GCCTTTGTGA ACGAGTGGTT GGAGATTGAG TTTCAGGGTT GCAGTTTTCT CTGGCACGAT 
GAGCCAGTGA CCACTCCAGT GCCCAATTTC CTTAGGGACT CACCTGCTCC GCAATCCTGG 
ACAGAAACGA GTAGCAACAA GCGCGTATAT CGACAGTCGC ATTTGGAGAT CGATGTGAAC 
CAGAAGAGCG ATCGCATGGA GTGGGGCCAT GTGAAGCATC ACACAGTGCT GCAGCCGAGA 
TTCGCCTTTG AGATCGTGGT TGAGTGGGTC ACCTCATCGG GTCCCATAGT GGCTGACTTG 
ATTGGCGGCT GGATGCGCAA GGCGAATCAG TTCAATTTCC TGGTATCAGT GCCAGCGGAT 
CCAATGGCAG AGCCGTTCAC CAAGAAGTCG GATCCTCTGC GGGGACCCAT TTTCATTCCG 
CTTTGCACTA CATTCCTGCC CAATGGCGCT GCCCTCTTCG ATGAGTTTCC CGAGGAGAGC 
AGATCAGACA GAATGCTGTT CTTCCAGGAA GCCATTTTGG GCAAGTTTGG CTTCTTGCCC 
TGCGTTTTGG AGAAGAAGTA CAGCGTTGGC AAGGATCTGC CCAAGGAGTA CCAGTATGTG 
CACTGCACGG GCAACATGTT TGCCTTGATC CGTTGCGCTA CAAACAACTA CCAGGTGGAA 
TCGCCCATCC TGAAGGAGGC AAATGTCACG CGCTGCGTCT ATGGGCACAC CAACAACACG 
AACGTGCCCA AGAAAGTGGG TTTCTTGTGG GCGTGGAACC ACATGATTCC GAACAAGAAA 
TGGAAGGCCC AGACGATAAA CAACTCCGCG GACGGGGAGC TCTTCCAGCT GAAGATGCTC 
AAGGATTTCC GCGAGTTTTG CTCGAACAGC GATCAGCGCC TGTCCACTTT CTGGACCCAA 
TCGCAGGAGT TGAAGCGAAG GGCCCAGAAA TTCGAGTTTA ACAATAACAA CACTGAGGAG 
AATAAGATGA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_728621.1
CDS	127..4761
Translation	MKLYKLNTHTRGCNKSYDADLVMNLKDHPNANVGDVVEIYAPDEENGTHLLLQITEFNGSCGRDVISIESGIANAFKMRPYSNVVMRIVKPADVALDSIEITFKDQYMGRSEMWRLKTYLTDTCVYVNKKIDYNDMQIRCQVYEMWSQGERVASGVITEDTKIVFRSSTSMVYLFLQMSSEMWDFDIHGDLYFEKAVNGFLTELFQKWRKLSCNHEVTIVLFSRTFYAAKSLDEFPEHMRDCLQQDYKGRFYEDFYRVAIQNERCDDWCTVLGQLRKLFTSYQATVLRYHERENMKIPPATNSSATQGNFLEVLNISLNTFEKHYLDRTFDRTGQLSVVITPGVGVFSVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDTTLTSADDYSLPHWINLSFYSTNKKIVYSSFIPRIKLPLFGSQLTLHDGVGDGEGEENERHFLSCNQSEYKHNSLFDYDAYDEQIFQPLPAQSTCSLQRVVRAKKTSVPSLETYAYRNNDWENLTPTQIPAMRRKMSDPDIHHGTSAMLAALQPDTTNLSESLASEKNSRRTIVSIAPIVRPGRALINPFDPSQVTIKLTSNRRRWTHIFPKGPTGVLIQQHHYQAVPAKPTQAGQQRPLQQTQSNNNNDQEDYGCENGEQYDRVSSHSLLNKSDSSQSFVMGDEPIDFFKRRQNSPMNPQPANVPNLTATQAKSYLWGATGEQEWTPAITTVKHLRPIVEGEHHHLGSLEALRALDPPPDAEAGGGRGKIIIGVDWKSLTIPACLPITTDYFPDKRSLNNDYVISDYTLLPDDVNHDYAQSRAVYRKPLSTEEVCKEIVSQRLAQGFQLIVVDEKPPTASGCSSGSAVQPVKLSRETNKEYLLSIGRIFHKISLSGSVITVTGYRPRHPYPPINVDYRYRFHAPQHETYEISGVNFTTEKLENFNWNHMDLYICTRGDVDYPLMESLKYWRYRMYLLPRENIVSKIASCQRCDIFPDVTADNTREQVEDFVRLIEAVSKLKRQYARKARYLASLLQDSPIAHITKRRHSTSIISRPQPNQGLTNSPFRERVGSNRLPEKRPSINVRPKLENGRIPRIFPATDAAAAAGVAARDDQDDGFPVDIKFSPNATLPEIFEAMKHPVNGVGFFSQTQSLPSCTFVSYDALMWLKTRLNNGRHPLDLLEAMRKERMICHASGDWKKPVVPGFVFYYVVQQDKNAKDYAPPLDDYSAFVNEWLEIEFQGCSFLWHDEPVTTPVPNFLRDSPAPQSWTETSSNKRVYRQSHLEIDVNQKSDRMEWGHVKHHTVLQPRFAFEIVVEWVTSSGPIVADLIGGWMRKANQFNFLVSVPADPMAEPFTKKSDPLRGPIFIPLCTTFLPNGAALFDEFPEESRSDRMLFFQEAILGKFGFLPCVLEKKYSVGKDLPKEYQYVHCTGNMFALIRCATNNYQVESPILKEANVTRCVYGHTNNTNVPKKVGFLWAWNHMIPNKKWKAQTINNSADGELFQLKMLKDFREFCSNSDQRLSTFWTQSQELKRRAQKFEFNNNNTEENKMK

Target ORF information:

RefSeq Version	NM_167889.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster increased minichromosome loss 1, transcript variant A (Iml1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_167889.2

ORF Insert Sequence:

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGTCA AGCATCTGAG GCCGATCGTC GAGGGCGAGC ATCATCATCT GGGCAGCCTG 
GAGGCACTAA GGGCTCTAGA CCCGCCCCCC GATGCGGAGG CGGGCGGCGG AAGAGGAAAG 
ATCATCATAG GCGTCGATTG GAAGTCGCTT ACGATCCCCG CCTGTCTGCC CATCACCACG 
GACTACTTCC CGGACAAGCG TTCGCTGAAT AACGACTATG TGATATCGGA TTACACTCTG 
CTGCCCGACG ATGTGAATCA CGATTACGCC CAGAGCAGAG CGGTTTACCG GAAACCCCTC 
TCCACGGAGG AGGTGTGCAA GGAGATCGTG TCGCAGCGCT TGGCGCAGGG CTTCCAGCTA 
ATCGTGGTCG ACGAGAAGCC TCCGACTGCG AGCGGTTGTT CCTCGGGATC TGCAGTGCAG 
CCGGTGAAGC TTTCGCGCGA GACCAACAAG GAGTATCTGC TCTCCATTGG CCGCATCTTT 
CACAAGATCT CGTTGAGCGG CTCGGTGATC ACGGTCACTG GCTATAGGCC CAGACACCCG 
TATCCTCCGA TAAATGTGGA CTACCGCTAC CGTTTCCATG CCCCACAACA CGAGACCTAC 
GAAATATCCG GCGTGAACTT CACCACCGAG AAGTTGGAGA ACTTCAACTG GAACCACATG 
GACCTGTACA TCTGCACGCG TGGCGATGTG GATTACCCAC TCATGGAGAG CCTGAAGTAC 
TGGCGCTATC GCATGTACCT GCTGCCCAGG GAGAACATTG TGAGCAAGAT CGCCAGTTGC 
CAGCGATGCG ACATATTCCC CGACGTGACT GCGGATAACA CGCGGGAGCA GGTCGAGGAC 
TTCGTCCGAC TGATCGAGGC GGTCAGCAAG CTGAAGCGGC AATATGCGCG CAAGGCGAGA 
TATCTCGCCT CACTCTTGCA GGACAGCCCC ATCGCCCACA TAACCAAGCG GAGACACAGC 
ACCAGCATTA TATCCAGGCC TCAGCCTAAC CAGGGACTCA CGAACTCTCC GTTCCGGGAG 
CGAGTCGGCA GCAATCGACT GCCGGAGAAG CGACCCAGCA TCAATGTGCG CCCCAAACTG 
GAGAACGGTC GAATTCCACG AATATTTCCC GCCACCGATG CAGCGGCAGC TGCAGGAGTC 
GCCGCCAGAG ATGATCAGGA CGATGGTTTC CCAGTCGACA TTAAGTTCAG CCCGAATGCC 
ACGCTGCCCG AAATCTTCGA GGCCATGAAG CACCCGGTAA ACGGAGTGGG CTTCTTCTCG 
CAGACGCAGT CGCTCCCCTC CTGCACCTTT GTGTCCTACG ACGCACTAAT GTGGTTGAAA 
ACCCGCCTGA ACAATGGACG ACATCCCCTG GATCTACTGG AGGCCATGCG CAAAGAGCGC 
ATGATCTGCC ATGCTTCGGG GGATTGGAAG AAGCCCGTGG TGCCTGGCTT TGTTTTCTAC 
TATGTGGTGC AGCAGGATAA AAACGCCAAA GATTACGCCC CGCCTTTGGA TGACTACAGT 
GCCTTTGTGA ACGAGTGGTT GGAGATTGAG TTTCAGGGTT GCAGTTTTCT CTGGCACGAT 
GAGCCAGTGA CCACTCCAGT GCCCAATTTC CTTAGGGACT CACCTGCTCC GCAATCCTGG 
ACAGAAACGA GTAGCAACAA GCGCGTATAT CGACAGTCGC ATTTGGAGAT CGATGTGAAC 
CAGAAGAGCG ATCGCATGGA GTGGGGCCAT GTGAAGCATC ACACAGTGCT GCAGCCGAGA 
TTCGCCTTTG AGATCGTGGT TGAGTGGGTC ACCTCATCGG GTCCCATAGT GGCTGACTTG 
ATTGGCGGCT GGATGCGCAA GGCGAATCAG TTCAATTTCC TGGTATCAGT GCCAGCGGAT 
CCAATGGCAG AGCCGTTCAC CAAGAAGTCG GATCCTCTGC GGGGACCCAT TTTCATTCCG 
CTTTGCACTA CATTCCTGCC CAATGGCGCT GCCCTCTTCG ATGAGTTTCC CGAGGAGAGC 
AGATCAGACA GAATGCTGTT CTTCCAGGAA GCCATTTTGG GCAAGTTTGG CTTCTTGCCC 
TGCGTTTTGG AGAAGAAGTA CAGCGTTGGC AAGGATCTGC CCAAGGAGTA CCAGTATGTG 
CACTGCACGG GCAACATGTT TGCCTTGATC CGTTGCGCTA CAAACAACTA CCAGGTGGAA 
TCGCCCATCC TGAAGGAGGC AAATGTCACG CGCTGCGTCT ATGGGCACAC CAACAACACG 
AACGTGCCCA AGAAAGTGGG TTTCTTGTGG GCGTGGAACC ACATGATTCC GAACAAGAAA 
TGGAAGGCCC AGACGATAAA CAACTCCGCG GACGGGGAGC TCTTCCAGCT GAAGATGCTC 
AAGGATTTCC GCGAGTTTTG CTCGAACAGC GATCAGCGCC TGTCCACTTT CTGGACCCAA 
TCGCAGGAGT TGAAGCGAAG GGCCCAGAAA TTCGAGTTTA ACAATAACAA CACTGAGGAG 
AATAAGATGA AATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iml1 ( NM_001274330.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261259.1 Drosophila melanogaster increased minichromosome loss 1, transcript variant D (Iml1), mRNA.

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CloneID	OFa20424
Clone ID Related Accession (Same CDS sequence)	NM_001274330.1
Accession Version	NM_001274330.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4536bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	increased minichromosome loss 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGTCA AGCATCTGAG GCCGATCGTC GAGGGCGAGC ATCATCATCT GGGCAGCCTG 
GAGGCACTAA GGGCTCTAGA CCCGCCCCCC GATGCGGAGG CGGGCGGCGG AAGAGGAAAG 
ATCATCATAG GCGTCGATTG GAAGTCGCTT ACGATCCCCG CCTGTCTGCC CATCACCACG 
GACTACTTCC CGGACAAGCG TTCGCTGAAT AACGACTATG TGATATCGGA TTACACTCTG 
CTGCCCGACG ATGTGAATCA CGATTACGCC CAGAGCAGAG CGGTTTACCG GAAACCCCTC 
TCCACGGAGG AGGTGTGCAA GGAGATCGTG TCGCAGCGCT TGGCGCAGGG CTTCCAGCTA 
ATCGTGGTCG ACGAGAAGCC TCCGACTGCG AGCGGTTGTT CCTCGGGATC TGCAGTGCAG 
CCGGTGAAGC TTTCGCGCGA GACCAACAAG GAGTATCTGC TCTCCATTGG CCGCATCTTT 
CACAAGATCT CGTTGAGCGG CTCGGTGATC ACGGTCACTG GCTATAGGCC CAGACACCCG 
TATCCTCCGA TAAATGTGGA CTACCGCTAC CGTTTCCATG CCCCACAACA CGAGACCTAC 
GAAATATCCG GCGTGAACTT CACCACCGAG AAGTTGGAGA ACTTCAACTG GAACCACATG 
GACCTGTACA TCTGCACGCG TGGCGATGTG GATTACCCAC TCATGGAGAG CCTGAAGTAC 
TGGCGCTATC GCATGTACCT GCTGCCCAGG GAGAACATTG TGAGCAAGAT CGCCAGTTGC 
CAGCGATGCG ACATATTCCC CGACGTGACT GCGGATAACA CGCGGGAGCA GGTCGAGGAC 
TTCGTCCGAC TGATCGAGGC GGTCAGCAAG CTGAAGCGGC AATATGCGCG CAAGGCGAGA 
CATGACACAC CTAGAATCAC TGAAAAGCAT CATAACCAAT TAAACTCACC GCTGCAAAGC 
ATCAATGTGC GCCCCAAACT GGAGAACGGT CGAATTCCAC GAATATTTCC CGCCACCGAT 
GCAGCGGCAG CTGCAGGAGT CGCCGCCAGA GATGATCAGG ACGATGGTTT CCCAGTCGAC 
ATTAAGTTCA GCCCGAATGC CACGCTGCCC GAAATCTTCG AGGCCATGAA GCACCCGGTA 
AACGGAGTGG GCTTCTTCTC GCAGACGCAG TCGCTCCCCT CCTGCACCTT TGTGTCCTAC 
GACGCACTAA TGTGGTTGAA AACCCGCCTG AACAATGGAC GACATCCCCT GGATCTACTG 
GAGGCCATGC GCAAAGAGCG CATGATCTGC CATGCTTCGG GGGATTGGAA GAAGCCCGTG 
GTGCCTGGCT TTGTTTTCTA CTATGTGGTG CAGCAGGATA AAAACGCCAA AGATTACGCC 
CCGCCTTTGG ATGACTACAG TGCCTTTGTG AACGAGTGGT TGGAGATTGA GTTTCAGGGT 
TGCAGTTTTC TCTGGCACGA TGAGCCAGTG ACCACTCCAG TGCCCAATTT CCTTAGGGAC 
TCACCTGCTC CGCAATCCTG GACAGAAACG AGTAGCAACA AGCGCGTATA TCGACAGTCG 
CATTTGGAGA TCGATGTGAA CCAGAAGAGC GATCGCATGG AGTGGGGCCA TGTGAAGCAT 
CACACAGTGC TGCAGCCGAG ATTCGCCTTT GAGATCGTGG TTGAGTGGGT CACCTCATCG 
GGTCCCATAG TGGCTGACTT GATTGGCGGC TGGATGCGCA AGGCGAATCA GTTCAATTTC 
CTGGTATCAG TGCCAGCGGA TCCAATGGCA GAGCCGTTCA CCAAGAAGTC GGATCCTCTG 
CGGGGACCCA TTTTCATTCC GCTTTGCACT ACATTCCTGC CCAATGGCGC TGCCCTCTTC 
GATGAGTTTC CCGAGGAGAG CAGATCAGAC AGAATGCTGT TCTTCCAGGA AGCCATTTTG 
GGCAAGTTTG GCTTCTTGCC CTGCGTTTTG GAGAAGAAGT ACAGCGTTGG CAAGGATCTG 
CCCAAGGAGT ACCAGTATGT GCACTGCACG GGCAACATGT TTGCCTTGAT CCGTTGCGCT 
ACAAACAACT ACCAGGTGGA ATCGCCCATC CTGAAGGAGG CAAATGTCAC GCGCTGCGTC 
TATGGGCACA CCAACAACAC GAACGTGCCC AAGAAAGTGG GTTTCTTGTG GGCGTGGAAC 
CACATGATTC CGAACAAGAA ATGGAAGGCC CAGACGATAA ACAACTCCGC GGACGGGGAG 
CTCTTCCAGC TGAAGATGCT CAAGGATTTC CGCGAGTTTT GCTCGAACAG CGATCAGCGC 
CTGTCCACTT TCTGGACCCA ATCGCAGGAG TTGAAGCGAA GGGCCCAGAA ATTCGAGTTT 
AACAATAACA ACACTGAGGA GAATAAGATG AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261259.1
CDS	127..4662
Translation	MKLYKLNTHTRGCNKSYDADLVMNLKDHPNANVGDVVEIYAPDEENGTHLLLQITEFNGSCGRDVISIESGIANAFKMRPYSNVVMRIVKPADVALDSIEITFKDQYMGRSEMWRLKTYLTDTCVYVNKKIDYNDMQIRCQVYEMWSQGERVASGVITEDTKIVFRSSTSMVYLFLQMSSEMWDFDIHGDLYFEKAVNGFLTELFQKWRKLSCNHEVTIVLFSRTFYAAKSLDEFPEHMRDCLQQDYKGRFYEDFYRVAIQNERCDDWCTVLGQLRKLFTSYQATVLRYHERENMKIPPATNSSATQGNFLEVLNISLNTFEKHYLDRTFDRTGQLSVVITPGVGVFSVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDTTLTSADDYSLPHWINLSFYSTNKKIVYSSFIPRIKLPLFGSQLTLHDGVGDGEGEENERHFLSCNQSEYKHNSLFDYDAYDEQIFQPLPAQSTCSLQRVVRAKKTSVPSLETYAYRNNDWENLTPTQIPAMRRKMSDPDIHHGTSAMLAALQPDTTNLSESLASEKNSRRTIVSIAPIVRPGRALINPFDPSQVTIKLTSNRRRWTHIFPKGPTGVLIQQHHYQAVPAKPTQAGQQRPLQQTQSNNNNDQEDYGCENGEQYDRVSSHSLLNKSDSSQSFVMGDEPIDFFKRRQNSPMNPQPANVPNLTATQAKSYLWGATGEQEWTPAITTVKHLRPIVEGEHHHLGSLEALRALDPPPDAEAGGGRGKIIIGVDWKSLTIPACLPITTDYFPDKRSLNNDYVISDYTLLPDDVNHDYAQSRAVYRKPLSTEEVCKEIVSQRLAQGFQLIVVDEKPPTASGCSSGSAVQPVKLSRETNKEYLLSIGRIFHKISLSGSVITVTGYRPRHPYPPINVDYRYRFHAPQHETYEISGVNFTTEKLENFNWNHMDLYICTRGDVDYPLMESLKYWRYRMYLLPRENIVSKIASCQRCDIFPDVTADNTREQVEDFVRLIEAVSKLKRQYARKARHDTPRITEKHHNQLNSPLQSINVRPKLENGRIPRIFPATDAAAAAGVAARDDQDDGFPVDIKFSPNATLPEIFEAMKHPVNGVGFFSQTQSLPSCTFVSYDALMWLKTRLNNGRHPLDLLEAMRKERMICHASGDWKKPVVPGFVFYYVVQQDKNAKDYAPPLDDYSAFVNEWLEIEFQGCSFLWHDEPVTTPVPNFLRDSPAPQSWTETSSNKRVYRQSHLEIDVNQKSDRMEWGHVKHHTVLQPRFAFEIVVEWVTSSGPIVADLIGGWMRKANQFNFLVSVPADPMAEPFTKKSDPLRGPIFIPLCTTFLPNGAALFDEFPEESRSDRMLFFQEAILGKFGFLPCVLEKKYSVGKDLPKEYQYVHCTGNMFALIRCATNNYQVESPILKEANVTRCVYGHTNNTNVPKKVGFLWAWNHMIPNKKWKAQTINNSADGELFQLKMLKDFREFCSNSDQRLSTFWTQSQELKRRAQKFEFNNNNTEENKMK

Target ORF information:

RefSeq Version	NM_001274330.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster increased minichromosome loss 1, transcript variant D (Iml1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274330.1

ORF Insert Sequence:

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGTCA AGCATCTGAG GCCGATCGTC GAGGGCGAGC ATCATCATCT GGGCAGCCTG 
GAGGCACTAA GGGCTCTAGA CCCGCCCCCC GATGCGGAGG CGGGCGGCGG AAGAGGAAAG 
ATCATCATAG GCGTCGATTG GAAGTCGCTT ACGATCCCCG CCTGTCTGCC CATCACCACG 
GACTACTTCC CGGACAAGCG TTCGCTGAAT AACGACTATG TGATATCGGA TTACACTCTG 
CTGCCCGACG ATGTGAATCA CGATTACGCC CAGAGCAGAG CGGTTTACCG GAAACCCCTC 
TCCACGGAGG AGGTGTGCAA GGAGATCGTG TCGCAGCGCT TGGCGCAGGG CTTCCAGCTA 
ATCGTGGTCG ACGAGAAGCC TCCGACTGCG AGCGGTTGTT CCTCGGGATC TGCAGTGCAG 
CCGGTGAAGC TTTCGCGCGA GACCAACAAG GAGTATCTGC TCTCCATTGG CCGCATCTTT 
CACAAGATCT CGTTGAGCGG CTCGGTGATC ACGGTCACTG GCTATAGGCC CAGACACCCG 
TATCCTCCGA TAAATGTGGA CTACCGCTAC CGTTTCCATG CCCCACAACA CGAGACCTAC 
GAAATATCCG GCGTGAACTT CACCACCGAG AAGTTGGAGA ACTTCAACTG GAACCACATG 
GACCTGTACA TCTGCACGCG TGGCGATGTG GATTACCCAC TCATGGAGAG CCTGAAGTAC 
TGGCGCTATC GCATGTACCT GCTGCCCAGG GAGAACATTG TGAGCAAGAT CGCCAGTTGC 
CAGCGATGCG ACATATTCCC CGACGTGACT GCGGATAACA CGCGGGAGCA GGTCGAGGAC 
TTCGTCCGAC TGATCGAGGC GGTCAGCAAG CTGAAGCGGC AATATGCGCG CAAGGCGAGA 
CATGACACAC CTAGAATCAC TGAAAAGCAT CATAACCAAT TAAACTCACC GCTGCAAAGC 
ATCAATGTGC GCCCCAAACT GGAGAACGGT CGAATTCCAC GAATATTTCC CGCCACCGAT 
GCAGCGGCAG CTGCAGGAGT CGCCGCCAGA GATGATCAGG ACGATGGTTT CCCAGTCGAC 
ATTAAGTTCA GCCCGAATGC CACGCTGCCC GAAATCTTCG AGGCCATGAA GCACCCGGTA 
AACGGAGTGG GCTTCTTCTC GCAGACGCAG TCGCTCCCCT CCTGCACCTT TGTGTCCTAC 
GACGCACTAA TGTGGTTGAA AACCCGCCTG AACAATGGAC GACATCCCCT GGATCTACTG 
GAGGCCATGC GCAAAGAGCG CATGATCTGC CATGCTTCGG GGGATTGGAA GAAGCCCGTG 
GTGCCTGGCT TTGTTTTCTA CTATGTGGTG CAGCAGGATA AAAACGCCAA AGATTACGCC 
CCGCCTTTGG ATGACTACAG TGCCTTTGTG AACGAGTGGT TGGAGATTGA GTTTCAGGGT 
TGCAGTTTTC TCTGGCACGA TGAGCCAGTG ACCACTCCAG TGCCCAATTT CCTTAGGGAC 
TCACCTGCTC CGCAATCCTG GACAGAAACG AGTAGCAACA AGCGCGTATA TCGACAGTCG 
CATTTGGAGA TCGATGTGAA CCAGAAGAGC GATCGCATGG AGTGGGGCCA TGTGAAGCAT 
CACACAGTGC TGCAGCCGAG ATTCGCCTTT GAGATCGTGG TTGAGTGGGT CACCTCATCG 
GGTCCCATAG TGGCTGACTT GATTGGCGGC TGGATGCGCA AGGCGAATCA GTTCAATTTC 
CTGGTATCAG TGCCAGCGGA TCCAATGGCA GAGCCGTTCA CCAAGAAGTC GGATCCTCTG 
CGGGGACCCA TTTTCATTCC GCTTTGCACT ACATTCCTGC CCAATGGCGC TGCCCTCTTC 
GATGAGTTTC CCGAGGAGAG CAGATCAGAC AGAATGCTGT TCTTCCAGGA AGCCATTTTG 
GGCAAGTTTG GCTTCTTGCC CTGCGTTTTG GAGAAGAAGT ACAGCGTTGG CAAGGATCTG 
CCCAAGGAGT ACCAGTATGT GCACTGCACG GGCAACATGT TTGCCTTGAT CCGTTGCGCT 
ACAAACAACT ACCAGGTGGA ATCGCCCATC CTGAAGGAGG CAAATGTCAC GCGCTGCGTC 
TATGGGCACA CCAACAACAC GAACGTGCCC AAGAAAGTGG GTTTCTTGTG GGCGTGGAAC 
CACATGATTC CGAACAAGAA ATGGAAGGCC CAGACGATAA ACAACTCCGC GGACGGGGAG 
CTCTTCCAGC TGAAGATGCT CAAGGATTTC CGCGAGTTTT GCTCGAACAG CGATCAGCGC 
CTGTCCACTT TCTGGACCCA ATCGCAGGAG TTGAAGCGAA GGGCCCAGAA ATTCGAGTTT 
AACAATAACA ACACTGAGGA GAATAAGATG AAATAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Iml1 ( NM_001274331.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001261260.1 Drosophila melanogaster increased minichromosome loss 1, transcript variant E (Iml1), mRNA.

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CloneID	OFa20434
Clone ID Related Accession (Same CDS sequence)	NM_001274331.1
Accession Version	NM_001274331.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4512bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	increased minichromosome loss 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_037436). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGGCG TCGATTGGAA GTCGCTTACG ATCCCCGCCT GTCTGCCCAT CACCACGGAC 
TACTTCCCGG ACAAGCGTTC GCTGAATAAC GACTATGTGA TATCGGATTA CACTCTGCTG 
CCCGACGATG TGAATCACGA TTACGCCCAG AGCAGAGCGG TTTACCGGAA ACCCCTCTCC 
ACGGAGGAGG TGTGCAAGGA GATCGTGTCG CAGCGCTTGG CGCAGGGCTT CCAGCTAATC 
GTGGTCGACG AGAAGCCTCC GACTGCGAGC GGTTGTTCCT CGGGATCTGC AGTGCAGCCG 
GTGAAGCTTT CGCGCGAGAC CAACAAGGAG TATCTGCTCT CCATTGGCCG CATCTTTCAC 
AAGATCTCGT TGAGCGGCTC GGTGATCACG GTCACTGGCT ATAGGCCCAG ACACCCGTAT 
CCTCCGATAA ATGTGGACTA CCGCTACCGT TTCCATGCCC CACAACACGA GACCTACGAA 
ATATCCGGCG TGAACTTCAC CACCGAGAAG TTGGAGAACT TCAACTGGAA CCACATGGAC 
CTGTACATCT GCACGCGTGG CGATGTGGAT TACCCACTCA TGGAGAGCCT GAAGTACTGG 
CGCTATCGCA TGTACCTGCT GCCCAGGGAG AACATTGTGA GCAAGATCGC CAGTTGCCAG 
CGATGCGACA TATTCCCCGA CGTGACTGCG GATAACACGC GGGAGCAGGT CGAGGACTTC 
GTCCGACTGA TCGAGGCGGT CAGCAAGCTG AAGCGGCAAT ATGCGCGCAA GGCGAGATAT 
CTCGCCTCAC TCTTGCAGGA CAGCCCCATC GCCCACATAA CCAAGCGGAG ACACAGCACC 
AGCATTATAT CCAGGCCTCA GCCTAACCAG GGACTCACGA ACTCTCCGTT CCGGGAGCGA 
GTCGGCAGCA ATCGACTGCC GGAGAAGCGA CCCAGCATCA ATGTGCGCCC CAAACTGGAG 
AACGGTCGAA TTCCACGAAT ATTTCCCGCC ACCGATGCAG CGGCAGCTGC AGGAGTCGCC 
GCCAGAGATG ATCAGGACGA TGGTTTCCCA GTCGACATTA AGTTCAGCCC GAATGCCACG 
CTGCCCGAAA TCTTCGAGGC CATGAAGCAC CCGGTAAACG GAGTGGGCTT CTTCTCGCAG 
ACGCAGTCGC TCCCCTCCTG CACCTTTGTG TCCTACGACG CACTAATGTG GTTGAAAACC 
CGCCTGAACA ATGGACGACA TCCCCTGGAT CTACTGGAGG CCATGCGCAA AGAGCGCATG 
ATCTGCCATG CTTCGGGGGA TTGGAAGAAG CCCGTGGTGC CTGGCTTTGT TTTCTACTAT 
GTGGTGCAGC AGGATAAAAA CGCCAAAGAT TACGCCCCGC CTTTGGATGA CTACAGTGCC 
TTTGTGAACG AGTGGTTGGA GATTGAGTTT CAGGGTTGCA GTTTTCTCTG GCACGATGAG 
CCAGTGACCA CTCCAGTGCC CAATTTCCTT AGGGACTCAC CTGCTCCGCA ATCCTGGACA 
GAAACGAGTA GCAACAAGCG CGTATATCGA CAGTCGCATT TGGAGATCGA TGTGAACCAG 
AAGAGCGATC GCATGGAGTG GGGCCATGTG AAGCATCACA CAGTGCTGCA GCCGAGATTC 
GCCTTTGAGA TCGTGGTTGA GTGGGTCACC TCATCGGGTC CCATAGTGGC TGACTTGATT 
GGCGGCTGGA TGCGCAAGGC GAATCAGTTC AATTTCCTGG TATCAGTGCC AGCGGATCCA 
ATGGCAGAGC CGTTCACCAA GAAGTCGGAT CCTCTGCGGG GACCCATTTT CATTCCGCTT 
TGCACTACAT TCCTGCCCAA TGGCGCTGCC CTCTTCGATG AGTTTCCCGA GGAGAGCAGA 
TCAGACAGAA TGCTGTTCTT CCAGGAAGCC ATTTTGGGCA AGTTTGGCTT CTTGCCCTGC 
GTTTTGGAGA AGAAGTACAG CGTTGGCAAG GATCTGCCCA AGGAGTACCA GTATGTGCAC 
TGCACGGGCA ACATGTTTGC CTTGATCCGT TGCGCTACAA ACAACTACCA GGTGGAATCG 
CCCATCCTGA AGGAGGCAAA TGTCACGCGC TGCGTCTATG GGCACACCAA CAACACGAAC 
GTGCCCAAGA AAGTGGGTTT CTTGTGGGCG TGGAACCACA TGATTCCGAA CAAGAAATGG 
AAGGCCCAGA CGATAAACAA CTCCGCGGAC GGGGAGCTCT TCCAGCTGAA GATGCTCAAG 
GATTTCCGCG AGTTTTGCTC GAACAGCGAT CAGCGCCTGT CCACTTTCTG GACCCAATCG 
CAGGAGTTGA AGCGAAGGGC CCAGAAATTC GAGTTTAACA ATAACAACAC TGAGGAGAAT 
AAGATGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001261260.1
CDS	127..4638
Translation	MKLYKLNTHTRGCNKSYDADLVMNLKDHPNANVGDVVEIYAPDEENGTHLLLQITEFNGSCGRDVISIESGIANAFKMRPYSNVVMRIVKPADVALDSIEITFKDQYMGRSEMWRLKTYLTDTCVYVNKKIDYNDMQIRCQVYEMWSQGERVASGVITEDTKIVFRSSTSMVYLFLQMSSEMWDFDIHGDLYFEKAVNGFLTELFQKWRKLSCNHEVTIVLFSRTFYAAKSLDEFPEHMRDCLQQDYKGRFYEDFYRVAIQNERCDDWCTVLGQLRKLFTSYQATVLRYHERENMKIPPATNSSATQGNFLEVLNISLNTFEKHYLDRTFDRTGQLSVVITPGVGVFSVDRELTNITKQRIIDNGVGSDLVCVGEQPLHAVPLLKFHNKDTTLTSADDYSLPHWINLSFYSTNKKIVYSSFIPRIKLPLFGSQLTLHDGVGDGEGEENERHFLSCNQSEYKHNSLFDYDAYDEQIFQPLPAQSTCSLQRVVRAKKTSVPSLETYAYRNNDWENLTPTQIPAMRRKMSDPDIHHGTSAMLAALQPDTTNLSESLASEKNSRRTIVSIAPIVRPGRALINPFDPSQVTIKLTSNRRRWTHIFPKGPTGVLIQQHHYQAVPAKPTQAGQQRPLQQTQSNNNNDQEDYGCENGEQYDRVSSHSLLNKSDSSQSFVMGDEPIDFFKRRQNSPMNPQPANVPNLTATQAKSYLWGATGEQEWTPAITTGVDWKSLTIPACLPITTDYFPDKRSLNNDYVISDYTLLPDDVNHDYAQSRAVYRKPLSTEEVCKEIVSQRLAQGFQLIVVDEKPPTASGCSSGSAVQPVKLSRETNKEYLLSIGRIFHKISLSGSVITVTGYRPRHPYPPINVDYRYRFHAPQHETYEISGVNFTTEKLENFNWNHMDLYICTRGDVDYPLMESLKYWRYRMYLLPRENIVSKIASCQRCDIFPDVTADNTREQVEDFVRLIEAVSKLKRQYARKARYLASLLQDSPIAHITKRRHSTSIISRPQPNQGLTNSPFRERVGSNRLPEKRPSINVRPKLENGRIPRIFPATDAAAAAGVAARDDQDDGFPVDIKFSPNATLPEIFEAMKHPVNGVGFFSQTQSLPSCTFVSYDALMWLKTRLNNGRHPLDLLEAMRKERMICHASGDWKKPVVPGFVFYYVVQQDKNAKDYAPPLDDYSAFVNEWLEIEFQGCSFLWHDEPVTTPVPNFLRDSPAPQSWTETSSNKRVYRQSHLEIDVNQKSDRMEWGHVKHHTVLQPRFAFEIVVEWVTSSGPIVADLIGGWMRKANQFNFLVSVPADPMAEPFTKKSDPLRGPIFIPLCTTFLPNGAALFDEFPEESRSDRMLFFQEAILGKFGFLPCVLEKKYSVGKDLPKEYQYVHCTGNMFALIRCATNNYQVESPILKEANVTRCVYGHTNNTNVPKKVGFLWAWNHMIPNKKWKAQTINNSADGELFQLKMLKDFREFCSNSDQRLSTFWTQSQELKRRAQKFEFNNNNTEENKMK

Target ORF information:

RefSeq Version	NM_001274331.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster increased minichromosome loss 1, transcript variant E (Iml1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001274331.1

ORF Insert Sequence:

ATGAAGCTGT ACAAGCTGAA CACGCATACG CGTGGCTGCA ACAAATCCTA CGATGCGGAC 
TTGGTGATGA ATCTAAAGGA TCATCCCAAC GCCAATGTGG GCGATGTGGT AGAAATTTAC 
GCCCCCGACG AGGAGAACGG CACCCACCTG CTGCTCCAAA TCACCGAGTT CAATGGCAGC 
TGTGGCCGGG ATGTGATAAG CATAGAATCG GGCATCGCCA ATGCCTTCAA AATGCGTCCG 
TACTCCAATG TGGTGATGCG CATAGTTAAG CCGGCGGATG TGGCCCTGGA CTCGATAGAG 
ATTACCTTCA AGGACCAGTA CATGGGTCGC TCGGAAATGT GGCGCCTGAA GACCTACCTG 
ACGGACACCT GTGTGTATGT GAACAAGAAG ATCGACTACA ACGACATGCA GATTCGGTGC 
CAGGTGTACG AGATGTGGTC GCAAGGCGAG CGCGTGGCCA GCGGTGTCAT CACGGAGGAC 
ACCAAGATTG TCTTCCGCAG CAGCACTTCG ATGGTGTACC TCTTCCTGCA GATGTCCTCC 
GAGATGTGGG ACTTTGACAT CCACGGAGAC CTGTACTTCG AAAAGGCAGT CAATGGGTTT 
CTCACTGAGC TCTTTCAAAA GTGGCGAAAA TTGAGCTGTA ACCACGAGGT GACCATCGTG 
CTGTTCTCCC GCACCTTCTA CGCAGCCAAG AGCCTGGACG AGTTTCCCGA GCATATGCGC 
GACTGCCTGC AGCAGGACTA CAAGGGTCGC TTCTACGAGG ACTTTTACCG CGTGGCCATC 
CAGAATGAGC GCTGTGATGA TTGGTGTACC GTTTTGGGCC AGCTCCGAAA GCTATTCACG 
TCCTACCAGG CCACTGTGCT GCGCTACCAC GAACGGGAGA ACATGAAGAT TCCACCGGCC 
ACCAATTCTT CAGCCACTCA GGGTAACTTT TTGGAGGTGC TAAACATATC GCTGAACACT 
TTCGAAAAGC ATTACTTGGA TAGAACGTTC GACCGCACCG GACAACTATC CGTGGTTATA 
ACCCCAGGAG TGGGTGTCTT TTCCGTGGAT CGAGAGCTAA CCAATATCAC GAAGCAGCGC 
ATTATCGACA ATGGCGTGGG TAGCGACCTG GTCTGTGTGG GCGAGCAGCC ACTGCATGCG 
GTTCCGCTTC TCAAATTCCA CAACAAAGAC ACCACGTTGA CCTCCGCCGA TGACTACTCT 
CTGCCCCACT GGATAAACTT GAGCTTTTAC TCCACTAACA AGAAGATTGT GTACTCCAGT 
TTCATACCAC GGATCAAGCT GCCTCTGTTT GGATCCCAGT TGACGCTCCA CGATGGCGTT 
GGCGACGGGG AGGGCGAGGA GAACGAGCGT CACTTCCTGA GCTGCAATCA GTCGGAGTAT 
AAGCACAACT CGCTCTTTGA TTACGATGCG TATGATGAAC AGATCTTCCA GCCGCTCCCC 
GCTCAGAGTA CTTGCTCCCT GCAGCGTGTA GTGCGGGCTA AGAAGACATC GGTGCCCAGT 
TTAGAGACCT ATGCCTATAG GAACAACGAT TGGGAGAACC TCACGCCGAC TCAAATCCCG 
GCTATGCGGC GCAAGATGTC CGATCCCGAT ATTCATCACG GCACCTCTGC AATGCTGGCT 
GCCTTGCAAC CGGATACAAC CAACCTCTCC GAGTCACTGG CCTCGGAGAA GAACTCCCGC 
AGGACGATCG TTAGCATTGC GCCCATAGTG CGTCCGGGTC GCGCTCTGAT CAATCCCTTT 
GATCCCTCGC AAGTGACAAT AAAGCTGACC TCCAATCGCC GCCGCTGGAC GCACATATTC 
CCGAAGGGCC CAACCGGTGT ACTCATACAG CAGCATCATT ATCAAGCGGT GCCCGCCAAA 
CCCACGCAGG CTGGTCAGCA GAGACCATTG CAGCAGACGC AGAGCAACAA CAACAACGAC 
CAGGAGGATT ACGGGTGCGA AAATGGAGAG CAGTACGACC GAGTGTCCAG CCATTCGCTG 
CTCAACAAGT CAGACTCCTC GCAGAGCTTT GTGATGGGTG ATGAGCCCAT TGATTTCTTT 
AAGCGGCGTC AAAACTCGCC GATGAACCCC CAGCCCGCCA ATGTGCCCAA CCTGACGGCC 
ACCCAGGCGA AGTCTTACCT CTGGGGAGCC ACTGGGGAGC AAGAGTGGAC ACCAGCAATT 
ACCACGGGCG TCGATTGGAA GTCGCTTACG ATCCCCGCCT GTCTGCCCAT CACCACGGAC 
TACTTCCCGG ACAAGCGTTC GCTGAATAAC GACTATGTGA TATCGGATTA CACTCTGCTG 
CCCGACGATG TGAATCACGA TTACGCCCAG AGCAGAGCGG TTTACCGGAA ACCCCTCTCC 
ACGGAGGAGG TGTGCAAGGA GATCGTGTCG CAGCGCTTGG CGCAGGGCTT CCAGCTAATC 
GTGGTCGACG AGAAGCCTCC GACTGCGAGC GGTTGTTCCT CGGGATCTGC AGTGCAGCCG 
GTGAAGCTTT CGCGCGAGAC CAACAAGGAG TATCTGCTCT CCATTGGCCG CATCTTTCAC 
AAGATCTCGT TGAGCGGCTC GGTGATCACG GTCACTGGCT ATAGGCCCAG ACACCCGTAT 
CCTCCGATAA ATGTGGACTA CCGCTACCGT TTCCATGCCC CACAACACGA GACCTACGAA 
ATATCCGGCG TGAACTTCAC CACCGAGAAG TTGGAGAACT TCAACTGGAA CCACATGGAC 
CTGTACATCT GCACGCGTGG CGATGTGGAT TACCCACTCA TGGAGAGCCT GAAGTACTGG 
CGCTATCGCA TGTACCTGCT GCCCAGGGAG AACATTGTGA GCAAGATCGC CAGTTGCCAG 
CGATGCGACA TATTCCCCGA CGTGACTGCG GATAACACGC GGGAGCAGGT CGAGGACTTC 
GTCCGACTGA TCGAGGCGGT CAGCAAGCTG AAGCGGCAAT ATGCGCGCAA GGCGAGATAT 
CTCGCCTCAC TCTTGCAGGA CAGCCCCATC GCCCACATAA CCAAGCGGAG ACACAGCACC 
AGCATTATAT CCAGGCCTCA GCCTAACCAG GGACTCACGA ACTCTCCGTT CCGGGAGCGA 
GTCGGCAGCA ATCGACTGCC GGAGAAGCGA CCCAGCATCA ATGTGCGCCC CAAACTGGAG 
AACGGTCGAA TTCCACGAAT ATTTCCCGCC ACCGATGCAG CGGCAGCTGC AGGAGTCGCC 
GCCAGAGATG ATCAGGACGA TGGTTTCCCA GTCGACATTA AGTTCAGCCC GAATGCCACG 
CTGCCCGAAA TCTTCGAGGC CATGAAGCAC CCGGTAAACG GAGTGGGCTT CTTCTCGCAG 
ACGCAGTCGC TCCCCTCCTG CACCTTTGTG TCCTACGACG CACTAATGTG GTTGAAAACC 
CGCCTGAACA ATGGACGACA TCCCCTGGAT CTACTGGAGG CCATGCGCAA AGAGCGCATG 
ATCTGCCATG CTTCGGGGGA TTGGAAGAAG CCCGTGGTGC CTGGCTTTGT TTTCTACTAT 
GTGGTGCAGC AGGATAAAAA CGCCAAAGAT TACGCCCCGC CTTTGGATGA CTACAGTGCC 
TTTGTGAACG AGTGGTTGGA GATTGAGTTT CAGGGTTGCA GTTTTCTCTG GCACGATGAG 
CCAGTGACCA CTCCAGTGCC CAATTTCCTT AGGGACTCAC CTGCTCCGCA ATCCTGGACA 
GAAACGAGTA GCAACAAGCG CGTATATCGA CAGTCGCATT TGGAGATCGA TGTGAACCAG 
AAGAGCGATC GCATGGAGTG GGGCCATGTG AAGCATCACA CAGTGCTGCA GCCGAGATTC 
GCCTTTGAGA TCGTGGTTGA GTGGGTCACC TCATCGGGTC CCATAGTGGC TGACTTGATT 
GGCGGCTGGA TGCGCAAGGC GAATCAGTTC AATTTCCTGG TATCAGTGCC AGCGGATCCA 
ATGGCAGAGC CGTTCACCAA GAAGTCGGAT CCTCTGCGGG GACCCATTTT CATTCCGCTT 
TGCACTACAT TCCTGCCCAA TGGCGCTGCC CTCTTCGATG AGTTTCCCGA GGAGAGCAGA 
TCAGACAGAA TGCTGTTCTT CCAGGAAGCC ATTTTGGGCA AGTTTGGCTT CTTGCCCTGC 
GTTTTGGAGA AGAAGTACAG CGTTGGCAAG GATCTGCCCA AGGAGTACCA GTATGTGCAC 
TGCACGGGCA ACATGTTTGC CTTGATCCGT TGCGCTACAA ACAACTACCA GGTGGAATCG 
CCCATCCTGA AGGAGGCAAA TGTCACGCGC TGCGTCTATG GGCACACCAA CAACACGAAC 
GTGCCCAAGA AAGTGGGTTT CTTGTGGGCG TGGAACCACA TGATTCCGAA CAAGAAATGG 
AAGGCCCAGA CGATAAACAA CTCCGCGGAC GGGGAGCTCT TCCAGCTGAA GATGCTCAAG 
GATTTCCGCG AGTTTTGCTC GAACAGCGAT CAGCGCCTGT CCACTTTCTG GACCCAATCG 
CAGGAGTTGA AGCGAAGGGC CCAGAAATTC GAGTTTAACA ATAACAACAC TGAGGAGAAT 
AAGATGAAAT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Iml1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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