DEPDC5 cDNA ORF clone, Canis lupus familiaris(dog)

The following DEPDC5 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DEPDC5 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe297392 XM_022410191.1
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
OCe26563 XM_005636491.3
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26563 XM_005636490.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26564 XM_014108000.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26565 XM_014108001.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26566 XM_014108002.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26567 XM_014108003.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26568 XM_014108004.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26569 XM_014108006.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26570 XM_014108007.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26571 XM_014108008.2
Latest version!
Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
OCe26567 XM_014108003.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26568 XM_014108004.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26565 XM_014108001.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26571 XM_014108008.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
OCe26566 XM_014108002.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26569 XM_014108006.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26563 XM_005636491.2 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26563 XM_005636490.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26564 XM_014108000.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
OCe26570 XM_014108007.1 Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe297392
    Clone ID Related Accession (Same CDS sequence) XM_022410191.1
    Accession Version XM_022410191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGGAAT TGGTGTGGAC
    TTGGTGTGCA TGGGGGAGCA ACCATTACAT GCTGTCCCGC TGTTTAAGCT GCATAATCGA
    AGTGCACCTC GTGATTCTCG TCTGGGCGAT GACTATAATA TTCCTCACTG GATAAACCAC
    AGTTTCTATA CGTCCAAAAG TCAGCTCTTT TGTAACAGTT TCACCCCACG AATAAAACTG
    GCGGGAAAGA AGCCTGCCTC TGAGAAAGCA AAAAATGGCC GTGATACATC TCTCGGGACT
    CCAAAAGAAT CTGAGAACGC CCTTCCCATC CAAGTAGATT ATGATGCCTA TGATGCCCAA
    GTGTTCAGGC TGCCAGGCCC ATCCCGGGCT CAGCGCCTCG CCACCTGCAG GTCTGTGCGA
    GAACGGGACA GTCACAATCG AAAGAGTGCC AGCTCCTGTG ATGTCTCATC CAGCCCTTCC
    CTGCCCAGCC GTGCACTGCC CACTGAAGAA GTGAGGAGCC AGGCTTCTGA CGACAGCTCC
    CTGGGCAGGA GTGCCAATAT CCTGATGATC CCACACCCCC ACCTGCACCA GTATGAAGTC
    AGCAGTTCCT TGGGCTATAC CAGCACTCGA GATGTCCTGG AGAACATGAT GGAGCCACCA
    CAGCGAGACT CTAGCGCACC AGGGAGGTTC CATGTAGGCA GTGCGGAATC CATGCTGCAT
    GTTCGACCCG GGGGATACAC GCCCCAGCGG GCGCTCATTA ACCCCTTTGC CCCCTCACGA
    ATGCCCATGA AGCTCACATC CAACAGAAGA CGCTGGATGC ACACTTTTCC TGTGGGACCA
    TCCGGAGAGG CCATCCAGAT CCATCACCAG ACCCGACAGA ATATGGCGGA GCTACAGGGC
    AGCGGGCAGA GGGACCCAGC TCACTCCTCT GCAGAGCTGC TGGAGTTAGC ATATCAGGAA
    GCTGCTGGAA GGCACAGCAC TTCCCGCCAG CCTGGTGACA GCATGTCCTT CTTGAGCTTC
    AGTGGAACAG AAGAGCTTTC TGTCAGCCTG CTTAGCAACA GTGGTGCAGG TATGAATCCT
    AGAACTCAGA ATAAGGATTC TCTAGAGGAC AGTGTTTCTA CCTCTCCAGA CCCAATTCTG
    ACACTGTCTG CTCCCCCTGT AGTGCCAGGC TTCTGTTGCA CAGTTGGAGT GGACTGGAAG
    TCTCTCACTA CTCCGGCGTG CCTCCCCCTC ACCACCGACT ACTTCCCCGA CCGCCAGGGC
    CTGCAGAACG ACTACACAGA GGGCTGTTAC GATCTCCTTC CAGAAGCAGA TATTGACAGG
    AGGGATGAGG AGGGTGTGCA GATGACGGCC CAGCAGGTGT TTGAAGAGTT CATCTGCCAG
    CGTCTCATGC AGGGCTACCA AATCATAGTG CAGCCCAAGG CACAAAAATC CAACCCCGTC
    GTCCCACCCC CACTGAGCAG CAGCCCGCTC TATAGTCGAG GCCTTGTGTC CCGAAACCGC
    CCTGAGGAGG AGGACCAATA CTGGCTGAGT ATGGGCAGAA CGTTCCACAA AGTGACGCTG
    AAAGATAAGA TGATCACAGT GACACGTTAC CTTCCCAAGT ACCCTTATGA ATCTGCCCAG
    ATCCACTACA CCTACAGTCT CTGCCCTTCC CACTCAGACT CGGAGTTCGT CTCTTGTTGG
    GTGGAATTCT CCCATGAGCG GCTGGAGGAG TACAAGTGGA ATTACTTAGA TCAGTATATC
    TGTTCTGCTG GCTCTGAGGA CTTCAGCTTA ATTGAGTCTC TGAAGTTCTG GAGGACCCGC
    TTCCTGCTGC TGCCAGCCTG TGTCACTGCC ACTAAGCGCA TCACAGAAGG GGAGGCCCAC
    TGCGACATCT ATGGGGACAG GCCTCGGGCA GACGAGGAGG AATGGCAGCT CTTGGATGGT
    TTTATCCGCT TCGTGGAGGG TTTGAACCGG ATCCGCAGGC GACATCGCTC AGATCGCATG
    ATGCGGAAAG GGACCGCCAT GAAAGGTTTG CAGATGACTG GGCCCATTGC CACACACTCC
    CTTGAGTCGA CCGGCCCCCC AGTTGGGAAG AAGGGAACCT CAGCTCTGTC CGCCCTGTTG
    GAGATGGAGG CCAGTCAGAA GTGCCTAGGA GAACAGCAGG CAGCTGTGCA CGGTGGCAAG
    AGCGCCTCCC AGCCAGCTGA GAGCAGCAGC GTTGCCATGA CTCCCACCTA CGTGGACAGC
    CCACGAAAGG ACGGGGCCTT CTTTATGGAG TTTGTCCGCA GCCCTCGCAC AGCGTCATCC
    GCCTTCTACC CACAGGTGTC TTTGGACCAG ACGGCCACCC CGGTGTTAGA CAGCACCAGT
    TTAGGGGTAA GCACAGGCCA GTCTGTGGAC AGAGGCAACA GCCAGGCCTT TGGGAACTCC
    CAGAACTCAG GGGAACAGAG CTTCCCCTCT GCAAACTCCA GTGATAGCAG CTCTCAGCAG
    CTGGCAGCAA GCTCTTTAAC CTCATCCTCC ACCTTGACAG AGATCCTGGA AGCCATGAAG
    CACCCCTCGA CAGGGGTCCA GCTGCTCTCA GAACAGAAGG GCCTCTCACC ATGCTGCTTC
    ATCAGTGCGG AGGTGGTGCA CTGGCTGGTG AATAACGTGG AGGGTGTCCA GACCCAGGCG
    ATGGCTATCG ACATCATGCA GAAAATGCTG GAAGAGCAAC TCATCACACA CGCATCTGGT
    GAAGTCTGGC GGACCTTCAT CTATGGCTTC TATTTCTACA AGATAGTCAT GGACAAAGAA
    CCTGACCGAG TGGCCATGCA GCAGCCCACC ACCTGGCACA CGACGGCGGG GGAGGACTTC
    GCCAGCTTCC AGCGCAAGTG GTTTGAGGTG GCCTTTGTGG CCGAAGAGCT GTTGCACTCC
    GAGATCCCTG CTTTCCTCTT GCCCTGGCTG CCCAGCCGAC CAGCCTCCTA TGCCAGCAGG
    CACAGCTCCT TCAGCCGCAG CTTTGGAGGG CGGAGCCAGG CAGCTGCACT TTTAGCTGCC
    ACTGTCCCAG AGCAGAGGAC TGTGACCCTG GATGTGGACG TGAACAACCG CACAGACCGG
    CTGGAGTGGT GCAGCTGTTA TTATCATGGC AATTTCTCCC TGAATGCAGC TTTTGAGATC
    AAGCTCCACT GGATGGCTGT GACTGCTGCG GTCCTCTTCG AGATGGTCCA GGGTTGGCAT
    CGCAAAGCCA CTTCCTGCGG CTTTTTATTG GTCCCTGTTC TGGAGGGTCC CTTTGCGCTG
    CCCAGTTACC TGTATGGCGA TCCACTTCGA GCCCAGCTCT TCATCCCACT CAACACCAGC
    TGCCTGCTCA AGGAGGGCAG TGAGCACCTG TTTGATAGCT TCGAGCCAGA AACATACTGG
    GATCGAATGC ACCTTTTCCA GGAAGCCATT GCACACAGGT TTGGATTTGT GCAAGATAAA
    TATTCCGCCT CTGCTTTTAA CTTCCCTGCT GAGAACAAGC CTCAGTATAT CCATGTTACA
    GGAACAGTGT TCCTGCAGCT GCCTTACTCC AGGCGCAAGT TCTCCGGGCA GCAGCGGCGG
    CGGCGGAACT CCACCAGTTC CACCAACCAG AACTTGTTCT GTGAGGAGCG TGTTGGCTAC
    AACTGGGCCT ACAACACCAT GCTGACCAAG ACATGGCGTT CTAGTGCCAC GGGGGATGAG
    AAGTTTGCTG ATCGGCTGCT CAGGGATTTC ACGGACTTCT GCATCAACCG CGACAACCGG
    CTGGTCACAT TCTGGATGAG CTGCCTGGAG AAGATGCATG CCAGTGCCCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265899.1
    CDS216..4889
    Translation

    Target ORF information:

    RefSeq Version XM_022410191.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGGAAT TGGTGTGGAC
    TTGGTGTGCA TGGGGGAGCA ACCATTACAT GCTGTCCCGC TGTTTAAGCT GCATAATCGA
    AGTGCACCTC GTGATTCTCG TCTGGGCGAT GACTATAATA TTCCTCACTG GATAAACCAC
    AGTTTCTATA CGTCCAAAAG TCAGCTCTTT TGTAACAGTT TCACCCCACG AATAAAACTG
    GCGGGAAAGA AGCCTGCCTC TGAGAAAGCA AAAAATGGCC GTGATACATC TCTCGGGACT
    CCAAAAGAAT CTGAGAACGC CCTTCCCATC CAAGTAGATT ATGATGCCTA TGATGCCCAA
    GTGTTCAGGC TGCCAGGCCC ATCCCGGGCT CAGCGCCTCG CCACCTGCAG GTCTGTGCGA
    GAACGGGACA GTCACAATCG AAAGAGTGCC AGCTCCTGTG ATGTCTCATC CAGCCCTTCC
    CTGCCCAGCC GTGCACTGCC CACTGAAGAA GTGAGGAGCC AGGCTTCTGA CGACAGCTCC
    CTGGGCAGGA GTGCCAATAT CCTGATGATC CCACACCCCC ACCTGCACCA GTATGAAGTC
    AGCAGTTCCT TGGGCTATAC CAGCACTCGA GATGTCCTGG AGAACATGAT GGAGCCACCA
    CAGCGAGACT CTAGCGCACC AGGGAGGTTC CATGTAGGCA GTGCGGAATC CATGCTGCAT
    GTTCGACCCG GGGGATACAC GCCCCAGCGG GCGCTCATTA ACCCCTTTGC CCCCTCACGA
    ATGCCCATGA AGCTCACATC CAACAGAAGA CGCTGGATGC ACACTTTTCC TGTGGGACCA
    TCCGGAGAGG CCATCCAGAT CCATCACCAG ACCCGACAGA ATATGGCGGA GCTACAGGGC
    AGCGGGCAGA GGGACCCAGC TCACTCCTCT GCAGAGCTGC TGGAGTTAGC ATATCAGGAA
    GCTGCTGGAA GGCACAGCAC TTCCCGCCAG CCTGGTGACA GCATGTCCTT CTTGAGCTTC
    AGTGGAACAG AAGAGCTTTC TGTCAGCCTG CTTAGCAACA GTGGTGCAGG TATGAATCCT
    AGAACTCAGA ATAAGGATTC TCTAGAGGAC AGTGTTTCTA CCTCTCCAGA CCCAATTCTG
    ACACTGTCTG CTCCCCCTGT AGTGCCAGGC TTCTGTTGCA CAGTTGGAGT GGACTGGAAG
    TCTCTCACTA CTCCGGCGTG CCTCCCCCTC ACCACCGACT ACTTCCCCGA CCGCCAGGGC
    CTGCAGAACG ACTACACAGA GGGCTGTTAC GATCTCCTTC CAGAAGCAGA TATTGACAGG
    AGGGATGAGG AGGGTGTGCA GATGACGGCC CAGCAGGTGT TTGAAGAGTT CATCTGCCAG
    CGTCTCATGC AGGGCTACCA AATCATAGTG CAGCCCAAGG CACAAAAATC CAACCCCGTC
    GTCCCACCCC CACTGAGCAG CAGCCCGCTC TATAGTCGAG GCCTTGTGTC CCGAAACCGC
    CCTGAGGAGG AGGACCAATA CTGGCTGAGT ATGGGCAGAA CGTTCCACAA AGTGACGCTG
    AAAGATAAGA TGATCACAGT GACACGTTAC CTTCCCAAGT ACCCTTATGA ATCTGCCCAG
    ATCCACTACA CCTACAGTCT CTGCCCTTCC CACTCAGACT CGGAGTTCGT CTCTTGTTGG
    GTGGAATTCT CCCATGAGCG GCTGGAGGAG TACAAGTGGA ATTACTTAGA TCAGTATATC
    TGTTCTGCTG GCTCTGAGGA CTTCAGCTTA ATTGAGTCTC TGAAGTTCTG GAGGACCCGC
    TTCCTGCTGC TGCCAGCCTG TGTCACTGCC ACTAAGCGCA TCACAGAAGG GGAGGCCCAC
    TGCGACATCT ATGGGGACAG GCCTCGGGCA GACGAGGAGG AATGGCAGCT CTTGGATGGT
    TTTATCCGCT TCGTGGAGGG TTTGAACCGG ATCCGCAGGC GACATCGCTC AGATCGCATG
    ATGCGGAAAG GGACCGCCAT GAAAGGTTTG CAGATGACTG GGCCCATTGC CACACACTCC
    CTTGAGTCGA CCGGCCCCCC AGTTGGGAAG AAGGGAACCT CAGCTCTGTC CGCCCTGTTG
    GAGATGGAGG CCAGTCAGAA GTGCCTAGGA GAACAGCAGG CAGCTGTGCA CGGTGGCAAG
    AGCGCCTCCC AGCCAGCTGA GAGCAGCAGC GTTGCCATGA CTCCCACCTA CGTGGACAGC
    CCACGAAAGG ACGGGGCCTT CTTTATGGAG TTTGTCCGCA GCCCTCGCAC AGCGTCATCC
    GCCTTCTACC CACAGGTGTC TTTGGACCAG ACGGCCACCC CGGTGTTAGA CAGCACCAGT
    TTAGGGGTAA GCACAGGCCA GTCTGTGGAC AGAGGCAACA GCCAGGCCTT TGGGAACTCC
    CAGAACTCAG GGGAACAGAG CTTCCCCTCT GCAAACTCCA GTGATAGCAG CTCTCAGCAG
    CTGGCAGCAA GCTCTTTAAC CTCATCCTCC ACCTTGACAG AGATCCTGGA AGCCATGAAG
    CACCCCTCGA CAGGGGTCCA GCTGCTCTCA GAACAGAAGG GCCTCTCACC ATGCTGCTTC
    ATCAGTGCGG AGGTGGTGCA CTGGCTGGTG AATAACGTGG AGGGTGTCCA GACCCAGGCG
    ATGGCTATCG ACATCATGCA GAAAATGCTG GAAGAGCAAC TCATCACACA CGCATCTGGT
    GAAGTCTGGC GGACCTTCAT CTATGGCTTC TATTTCTACA AGATAGTCAT GGACAAAGAA
    CCTGACCGAG TGGCCATGCA GCAGCCCACC ACCTGGCACA CGACGGCGGG GGAGGACTTC
    GCCAGCTTCC AGCGCAAGTG GTTTGAGGTG GCCTTTGTGG CCGAAGAGCT GTTGCACTCC
    GAGATCCCTG CTTTCCTCTT GCCCTGGCTG CCCAGCCGAC CAGCCTCCTA TGCCAGCAGG
    CACAGCTCCT TCAGCCGCAG CTTTGGAGGG CGGAGCCAGG CAGCTGCACT TTTAGCTGCC
    ACTGTCCCAG AGCAGAGGAC TGTGACCCTG GATGTGGACG TGAACAACCG CACAGACCGG
    CTGGAGTGGT GCAGCTGTTA TTATCATGGC AATTTCTCCC TGAATGCAGC TTTTGAGATC
    AAGCTCCACT GGATGGCTGT GACTGCTGCG GTCCTCTTCG AGATGGTCCA GGGTTGGCAT
    CGCAAAGCCA CTTCCTGCGG CTTTTTATTG GTCCCTGTTC TGGAGGGTCC CTTTGCGCTG
    CCCAGTTACC TGTATGGCGA TCCACTTCGA GCCCAGCTCT TCATCCCACT CAACACCAGC
    TGCCTGCTCA AGGAGGGCAG TGAGCACCTG TTTGATAGCT TCGAGCCAGA AACATACTGG
    GATCGAATGC ACCTTTTCCA GGAAGCCATT GCACACAGGT TTGGATTTGT GCAAGATAAA
    TATTCCGCCT CTGCTTTTAA CTTCCCTGCT GAGAACAAGC CTCAGTATAT CCATGTTACA
    GGAACAGTGT TCCTGCAGCT GCCTTACTCC AGGCGCAAGT TCTCCGGGCA GCAGCGGCGG
    CGGCGGAACT CCACCAGTTC CACCAACCAG AACTTGTTCT GTGAGGAGCG TGTTGGCTAC
    AACTGGGCCT ACAACACCAT GCTGACCAAG ACATGGCGTT CTAGTGCCAC GGGGGATGAG
    AAGTTTGCTG ATCGGCTGCT CAGGGATTTC ACGGACTTCT GCATCAACCG CGACAACCGG
    CTGGTCACAT TCTGGATGAG CTGCCTGGAG AAGATGCATG CCAGTGCCCC GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26563
    Clone ID Related Accession (Same CDS sequence) XM_005636491.2 , XM_005636490.1 , XM_005636491.3 , XM_005636490.2
    Accession Version XM_005636491.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005636491.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636548.1
    CDS228..5036
    Translation

    Target ORF information:

    RefSeq Version XM_005636491.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636491.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26563
    Clone ID Related Accession (Same CDS sequence) XM_005636491.2 , XM_005636490.1 , XM_005636491.3 , XM_005636490.2
    Accession Version XM_005636490.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636547.1
    CDS146..4954
    Translation

    Target ORF information:

    RefSeq Version XM_005636490.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636490.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26563
    Clone ID Related Accession (Same CDS sequence) XM_005636491.2 , XM_005636490.1 , XM_005636491.3 , XM_005636490.2
    Accession Version XM_005636491.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636491.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636548.1
    CDS216..5024
    Translation

    Target ORF information:

    RefSeq Version XM_005636491.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636491.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26563
    Clone ID Related Accession (Same CDS sequence) XM_005636491.2 , XM_005636490.1 , XM_005636491.3 , XM_005636490.2
    Accession Version XM_005636490.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4809bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005636490.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005636547.1
    CDS146..4954
    Translation

    Target ORF information:

    RefSeq Version XM_005636490.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005636490.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26564
    Clone ID Related Accession (Same CDS sequence) XM_014108000.1 , XM_014108000.2
    Accession Version XM_014108000.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGAC
    GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCTCGCACAG CGTCATCCGC CTTCTACCCA
    CAGGTGTCTT TGGACCAGAC GGCCACCCCG GTGTTAGACA GCACCAGTTT AGGGGTAAGC
    ACAGGCCAGT CTGTGGACAG AGGCAACAGC CAGGCCTTTG GGAACTCCCA GAACTCAGGG
    GAACAGAGCT TCCCCTCTGC AAACTCCAGT GATAGCAGCT CTCAGCAGCT GGCAGCAAGC
    TCTTTAACCT CATCCTCCAC CTTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA
    GGGGTCCAGC TGCTCTCAGA ACAGAAGGGC CTCTCACCAT GCTGCTTCAT CAGTGCGGAG
    GTGGTGCACT GGCTGGTGAA TAACGTGGAG GGTGTCCAGA CCCAGGCGAT GGCTATCGAC
    ATCATGCAGA AAATGCTGGA AGAGCAACTC ATCACACACG CATCTGGTGA AGTCTGGCGG
    ACCTTCATCT ATGGCTTCTA TTTCTACAAG ATAGTCATGG ACAAAGAACC TGACCGAGTG
    GCCATGCAGC AGCCCACCAC CTGGCACACG ACGGCGGGGG AGGACTTCGC CAGCTTCCAG
    CGCAAGTGGT TTGAGGTGGC CTTTGTGGCC GAAGAGCTGT TGCACTCCGA GATCCCTGCT
    TTCCTCTTGC CCTGGCTGCC CAGCCGACCA GCCTCCTATG CCAGCAGGCA CAGCTCCTTC
    AGCCGCAGCT TTGGAGGGCG GAGCCAGGCA GCTGCACTTT TAGCTGCCAC TGTCCCAGAG
    CAGAGGACTG TGACCCTGGA TGTGGACGTG AACAACCGCA CAGACCGGCT GGAGTGGTGC
    AGCTGTTATT ATCATGGCAA TTTCTCCCTG AATGCAGCTT TTGAGATCAA GCTCCACTGG
    ATGGCTGTGA CTGCTGCGGT CCTCTTCGAG ATGGTCCAGG GTTGGCATCG CAAAGCCACT
    TCCTGCGGCT TTTTATTGGT CCCTGTTCTG GAGGGTCCCT TTGCGCTGCC CAGTTACCTG
    TATGGCGATC CACTTCGAGC CCAGCTCTTC ATCCCACTCA ACACCAGCTG CCTGCTCAAG
    GAGGGCAGTG AGCACCTGTT TGATAGCTTC GAGCCAGAAA CATACTGGGA TCGAATGCAC
    CTTTTCCAGG AAGCCATTGC ACACAGGTTT GGATTTGTGC AAGATAAATA TTCCGCCTCT
    GCTTTTAACT TCCCTGCTGA GAACAAGCCT CAGTATATCC ATGTTACAGG AACAGTGTTC
    CTGCAGCTGC CTTACTCCAG GCGCAAGTTC TCCGGGCAGC AGCGGCGGCG GCGGAACTCC
    ACCAGTTCCA CCAACCAGAA CTTGTTCTGT GAGGAGCGTG TTGGCTACAA CTGGGCCTAC
    AACACCATGC TGACCAAGAC ATGGCGTTCT AGTGCCACGG GGGATGAGAA GTTTGCTGAT
    CGGCTGCTCA GGGATTTCAC GGACTTCTGC ATCAACCGCG ACAACCGGCT GGTCACATTC
    TGGATGAGCT GCCTGGAGAA GATGCATGCC AGTGCCCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963475.1
    CDS228..5009
    Translation

    Target ORF information:

    RefSeq Version XM_014108000.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108000.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGAC
    GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCTCGCACAG CGTCATCCGC CTTCTACCCA
    CAGGTGTCTT TGGACCAGAC GGCCACCCCG GTGTTAGACA GCACCAGTTT AGGGGTAAGC
    ACAGGCCAGT CTGTGGACAG AGGCAACAGC CAGGCCTTTG GGAACTCCCA GAACTCAGGG
    GAACAGAGCT TCCCCTCTGC AAACTCCAGT GATAGCAGCT CTCAGCAGCT GGCAGCAAGC
    TCTTTAACCT CATCCTCCAC CTTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA
    GGGGTCCAGC TGCTCTCAGA ACAGAAGGGC CTCTCACCAT GCTGCTTCAT CAGTGCGGAG
    GTGGTGCACT GGCTGGTGAA TAACGTGGAG GGTGTCCAGA CCCAGGCGAT GGCTATCGAC
    ATCATGCAGA AAATGCTGGA AGAGCAACTC ATCACACACG CATCTGGTGA AGTCTGGCGG
    ACCTTCATCT ATGGCTTCTA TTTCTACAAG ATAGTCATGG ACAAAGAACC TGACCGAGTG
    GCCATGCAGC AGCCCACCAC CTGGCACACG ACGGCGGGGG AGGACTTCGC CAGCTTCCAG
    CGCAAGTGGT TTGAGGTGGC CTTTGTGGCC GAAGAGCTGT TGCACTCCGA GATCCCTGCT
    TTCCTCTTGC CCTGGCTGCC CAGCCGACCA GCCTCCTATG CCAGCAGGCA CAGCTCCTTC
    AGCCGCAGCT TTGGAGGGCG GAGCCAGGCA GCTGCACTTT TAGCTGCCAC TGTCCCAGAG
    CAGAGGACTG TGACCCTGGA TGTGGACGTG AACAACCGCA CAGACCGGCT GGAGTGGTGC
    AGCTGTTATT ATCATGGCAA TTTCTCCCTG AATGCAGCTT TTGAGATCAA GCTCCACTGG
    ATGGCTGTGA CTGCTGCGGT CCTCTTCGAG ATGGTCCAGG GTTGGCATCG CAAAGCCACT
    TCCTGCGGCT TTTTATTGGT CCCTGTTCTG GAGGGTCCCT TTGCGCTGCC CAGTTACCTG
    TATGGCGATC CACTTCGAGC CCAGCTCTTC ATCCCACTCA ACACCAGCTG CCTGCTCAAG
    GAGGGCAGTG AGCACCTGTT TGATAGCTTC GAGCCAGAAA CATACTGGGA TCGAATGCAC
    CTTTTCCAGG AAGCCATTGC ACACAGGTTT GGATTTGTGC AAGATAAATA TTCCGCCTCT
    GCTTTTAACT TCCCTGCTGA GAACAAGCCT CAGTATATCC ATGTTACAGG AACAGTGTTC
    CTGCAGCTGC CTTACTCCAG GCGCAAGTTC TCCGGGCAGC AGCGGCGGCG GCGGAACTCC
    ACCAGTTCCA CCAACCAGAA CTTGTTCTGT GAGGAGCGTG TTGGCTACAA CTGGGCCTAC
    AACACCATGC TGACCAAGAC ATGGCGTTCT AGTGCCACGG GGGATGAGAA GTTTGCTGAT
    CGGCTGCTCA GGGATTTCAC GGACTTCTGC ATCAACCGCG ACAACCGGCT GGTCACATTC
    TGGATGAGCT GCCTGGAGAA GATGCATGCC AGTGCCCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26564
    Clone ID Related Accession (Same CDS sequence) XM_014108000.1 , XM_014108000.2
    Accession Version XM_014108000.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4782bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108000.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGAC
    GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCTCGCACAG CGTCATCCGC CTTCTACCCA
    CAGGTGTCTT TGGACCAGAC GGCCACCCCG GTGTTAGACA GCACCAGTTT AGGGGTAAGC
    ACAGGCCAGT CTGTGGACAG AGGCAACAGC CAGGCCTTTG GGAACTCCCA GAACTCAGGG
    GAACAGAGCT TCCCCTCTGC AAACTCCAGT GATAGCAGCT CTCAGCAGCT GGCAGCAAGC
    TCTTTAACCT CATCCTCCAC CTTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA
    GGGGTCCAGC TGCTCTCAGA ACAGAAGGGC CTCTCACCAT GCTGCTTCAT CAGTGCGGAG
    GTGGTGCACT GGCTGGTGAA TAACGTGGAG GGTGTCCAGA CCCAGGCGAT GGCTATCGAC
    ATCATGCAGA AAATGCTGGA AGAGCAACTC ATCACACACG CATCTGGTGA AGTCTGGCGG
    ACCTTCATCT ATGGCTTCTA TTTCTACAAG ATAGTCATGG ACAAAGAACC TGACCGAGTG
    GCCATGCAGC AGCCCACCAC CTGGCACACG ACGGCGGGGG AGGACTTCGC CAGCTTCCAG
    CGCAAGTGGT TTGAGGTGGC CTTTGTGGCC GAAGAGCTGT TGCACTCCGA GATCCCTGCT
    TTCCTCTTGC CCTGGCTGCC CAGCCGACCA GCCTCCTATG CCAGCAGGCA CAGCTCCTTC
    AGCCGCAGCT TTGGAGGGCG GAGCCAGGCA GCTGCACTTT TAGCTGCCAC TGTCCCAGAG
    CAGAGGACTG TGACCCTGGA TGTGGACGTG AACAACCGCA CAGACCGGCT GGAGTGGTGC
    AGCTGTTATT ATCATGGCAA TTTCTCCCTG AATGCAGCTT TTGAGATCAA GCTCCACTGG
    ATGGCTGTGA CTGCTGCGGT CCTCTTCGAG ATGGTCCAGG GTTGGCATCG CAAAGCCACT
    TCCTGCGGCT TTTTATTGGT CCCTGTTCTG GAGGGTCCCT TTGCGCTGCC CAGTTACCTG
    TATGGCGATC CACTTCGAGC CCAGCTCTTC ATCCCACTCA ACACCAGCTG CCTGCTCAAG
    GAGGGCAGTG AGCACCTGTT TGATAGCTTC GAGCCAGAAA CATACTGGGA TCGAATGCAC
    CTTTTCCAGG AAGCCATTGC ACACAGGTTT GGATTTGTGC AAGATAAATA TTCCGCCTCT
    GCTTTTAACT TCCCTGCTGA GAACAAGCCT CAGTATATCC ATGTTACAGG AACAGTGTTC
    CTGCAGCTGC CTTACTCCAG GCGCAAGTTC TCCGGGCAGC AGCGGCGGCG GCGGAACTCC
    ACCAGTTCCA CCAACCAGAA CTTGTTCTGT GAGGAGCGTG TTGGCTACAA CTGGGCCTAC
    AACACCATGC TGACCAAGAC ATGGCGTTCT AGTGCCACGG GGGATGAGAA GTTTGCTGAT
    CGGCTGCTCA GGGATTTCAC GGACTTCTGC ATCAACCGCG ACAACCGGCT GGTCACATTC
    TGGATGAGCT GCCTGGAGAA GATGCATGCC AGTGCCCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963475.1
    CDS216..4997
    Translation

    Target ORF information:

    RefSeq Version XM_014108000.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108000.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGAC
    GGGGCCTTCT TTATGGAGTT TGTCCGCAGC CCTCGCACAG CGTCATCCGC CTTCTACCCA
    CAGGTGTCTT TGGACCAGAC GGCCACCCCG GTGTTAGACA GCACCAGTTT AGGGGTAAGC
    ACAGGCCAGT CTGTGGACAG AGGCAACAGC CAGGCCTTTG GGAACTCCCA GAACTCAGGG
    GAACAGAGCT TCCCCTCTGC AAACTCCAGT GATAGCAGCT CTCAGCAGCT GGCAGCAAGC
    TCTTTAACCT CATCCTCCAC CTTGACAGAG ATCCTGGAAG CCATGAAGCA CCCCTCGACA
    GGGGTCCAGC TGCTCTCAGA ACAGAAGGGC CTCTCACCAT GCTGCTTCAT CAGTGCGGAG
    GTGGTGCACT GGCTGGTGAA TAACGTGGAG GGTGTCCAGA CCCAGGCGAT GGCTATCGAC
    ATCATGCAGA AAATGCTGGA AGAGCAACTC ATCACACACG CATCTGGTGA AGTCTGGCGG
    ACCTTCATCT ATGGCTTCTA TTTCTACAAG ATAGTCATGG ACAAAGAACC TGACCGAGTG
    GCCATGCAGC AGCCCACCAC CTGGCACACG ACGGCGGGGG AGGACTTCGC CAGCTTCCAG
    CGCAAGTGGT TTGAGGTGGC CTTTGTGGCC GAAGAGCTGT TGCACTCCGA GATCCCTGCT
    TTCCTCTTGC CCTGGCTGCC CAGCCGACCA GCCTCCTATG CCAGCAGGCA CAGCTCCTTC
    AGCCGCAGCT TTGGAGGGCG GAGCCAGGCA GCTGCACTTT TAGCTGCCAC TGTCCCAGAG
    CAGAGGACTG TGACCCTGGA TGTGGACGTG AACAACCGCA CAGACCGGCT GGAGTGGTGC
    AGCTGTTATT ATCATGGCAA TTTCTCCCTG AATGCAGCTT TTGAGATCAA GCTCCACTGG
    ATGGCTGTGA CTGCTGCGGT CCTCTTCGAG ATGGTCCAGG GTTGGCATCG CAAAGCCACT
    TCCTGCGGCT TTTTATTGGT CCCTGTTCTG GAGGGTCCCT TTGCGCTGCC CAGTTACCTG
    TATGGCGATC CACTTCGAGC CCAGCTCTTC ATCCCACTCA ACACCAGCTG CCTGCTCAAG
    GAGGGCAGTG AGCACCTGTT TGATAGCTTC GAGCCAGAAA CATACTGGGA TCGAATGCAC
    CTTTTCCAGG AAGCCATTGC ACACAGGTTT GGATTTGTGC AAGATAAATA TTCCGCCTCT
    GCTTTTAACT TCCCTGCTGA GAACAAGCCT CAGTATATCC ATGTTACAGG AACAGTGTTC
    CTGCAGCTGC CTTACTCCAG GCGCAAGTTC TCCGGGCAGC AGCGGCGGCG GCGGAACTCC
    ACCAGTTCCA CCAACCAGAA CTTGTTCTGT GAGGAGCGTG TTGGCTACAA CTGGGCCTAC
    AACACCATGC TGACCAAGAC ATGGCGTTCT AGTGCCACGG GGGATGAGAA GTTTGCTGAT
    CGGCTGCTCA GGGATTTCAC GGACTTCTGC ATCAACCGCG ACAACCGGCT GGTCACATTC
    TGGATGAGCT GCCTGGAGAA GATGCATGCC AGTGCCCCGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26565
    Clone ID Related Accession (Same CDS sequence) XM_014108001.1 , XM_014108001.2
    Accession Version XM_014108001.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGTGTCT TTGGACCAGA CGGCCACCCC GGTGTTAGAC
    AGCACCAGTT TAGGGGTAAG CACAGGCCAG TCTGTGGACA GAGGCAACAG CCAGGCCTTT
    GGGAACTCCC AGAACTCAGG GGAACAGAGC TTCCCCTCTG CAAACTCCAG TGATAGCAGC
    TCTCAGCAGC TGGCAGCAAG CTCTTTAACC TCATCCTCCA CCTTGACAGA GATCCTGGAA
    GCCATGAAGC ACCCCTCGAC AGGGGTCCAG CTGCTCTCAG AACAGAAGGG CCTCTCACCA
    TGCTGCTTCA TCAGTGCGGA GGTGGTGCAC TGGCTGGTGA ATAACGTGGA GGGTGTCCAG
    ACCCAGGCGA TGGCTATCGA CATCATGCAG AAAATGCTGG AAGAGCAACT CATCACACAC
    GCATCTGGTG AAGTCTGGCG GACCTTCATC TATGGCTTCT ATTTCTACAA GATAGTCATG
    GACAAAGAAC CTGACCGAGT GGCCATGCAG CAGCCCACCA CCTGGCACAC GACGGCGGGG
    GAGGACTTCG CCAGCTTCCA GCGCAAGTGG TTTGAGGTGG CCTTTGTGGC CGAAGAGCTG
    TTGCACTCCG AGATCCCTGC TTTCCTCTTG CCCTGGCTGC CCAGCCGACC AGCCTCCTAT
    GCCAGCAGGC ACAGCTCCTT CAGCCGCAGC TTTGGAGGGC GGAGCCAGGC AGCTGCACTT
    TTAGCTGCCA CTGTCCCAGA GCAGAGGACT GTGACCCTGG ATGTGGACGT GAACAACCGC
    ACAGACCGGC TGGAGTGGTG CAGCTGTTAT TATCATGGCA ATTTCTCCCT GAATGCAGCT
    TTTGAGATCA AGCTCCACTG GATGGCTGTG ACTGCTGCGG TCCTCTTCGA GATGGTCCAG
    GGTTGGCATC GCAAAGCCAC TTCCTGCGGC TTTTTATTGG TCCCTGTTCT GGAGGGTCCC
    TTTGCGCTGC CCAGTTACCT GTATGGCGAT CCACTTCGAG CCCAGCTCTT CATCCCACTC
    AACACCAGCT GCCTGCTCAA GGAGGGCAGT GAGCACCTGT TTGATAGCTT CGAGCCAGAA
    ACATACTGGG ATCGAATGCA CCTTTTCCAG GAAGCCATTG CACACAGGTT TGGATTTGTG
    CAAGATAAAT ATTCCGCCTC TGCTTTTAAC TTCCCTGCTG AGAACAAGCC TCAGTATATC
    CATGTTACAG GAACAGTGTT CCTGCAGCTG CCTTACTCCA GGCGCAAGTT CTCCGGGCAG
    CAGCGGCGGC GGCGGAACTC CACCAGTTCC ACCAACCAGA ACTTGTTCTG TGAGGAGCGT
    GTTGGCTACA ACTGGGCCTA CAACACCATG CTGACCAAGA CATGGCGTTC TAGTGCCACG
    GGGGATGAGA AGTTTGCTGA TCGGCTGCTC AGGGATTTCA CGGACTTCTG CATCAACCGC
    GACAACCGGC TGGTCACATT CTGGATGAGC TGCCTGGAGA AGATGCATGC CAGTGCCCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963476.1
    CDS228..4970
    Translation

    Target ORF information:

    RefSeq Version XM_014108001.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108001.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGTGTCT TTGGACCAGA CGGCCACCCC GGTGTTAGAC
    AGCACCAGTT TAGGGGTAAG CACAGGCCAG TCTGTGGACA GAGGCAACAG CCAGGCCTTT
    GGGAACTCCC AGAACTCAGG GGAACAGAGC TTCCCCTCTG CAAACTCCAG TGATAGCAGC
    TCTCAGCAGC TGGCAGCAAG CTCTTTAACC TCATCCTCCA CCTTGACAGA GATCCTGGAA
    GCCATGAAGC ACCCCTCGAC AGGGGTCCAG CTGCTCTCAG AACAGAAGGG CCTCTCACCA
    TGCTGCTTCA TCAGTGCGGA GGTGGTGCAC TGGCTGGTGA ATAACGTGGA GGGTGTCCAG
    ACCCAGGCGA TGGCTATCGA CATCATGCAG AAAATGCTGG AAGAGCAACT CATCACACAC
    GCATCTGGTG AAGTCTGGCG GACCTTCATC TATGGCTTCT ATTTCTACAA GATAGTCATG
    GACAAAGAAC CTGACCGAGT GGCCATGCAG CAGCCCACCA CCTGGCACAC GACGGCGGGG
    GAGGACTTCG CCAGCTTCCA GCGCAAGTGG TTTGAGGTGG CCTTTGTGGC CGAAGAGCTG
    TTGCACTCCG AGATCCCTGC TTTCCTCTTG CCCTGGCTGC CCAGCCGACC AGCCTCCTAT
    GCCAGCAGGC ACAGCTCCTT CAGCCGCAGC TTTGGAGGGC GGAGCCAGGC AGCTGCACTT
    TTAGCTGCCA CTGTCCCAGA GCAGAGGACT GTGACCCTGG ATGTGGACGT GAACAACCGC
    ACAGACCGGC TGGAGTGGTG CAGCTGTTAT TATCATGGCA ATTTCTCCCT GAATGCAGCT
    TTTGAGATCA AGCTCCACTG GATGGCTGTG ACTGCTGCGG TCCTCTTCGA GATGGTCCAG
    GGTTGGCATC GCAAAGCCAC TTCCTGCGGC TTTTTATTGG TCCCTGTTCT GGAGGGTCCC
    TTTGCGCTGC CCAGTTACCT GTATGGCGAT CCACTTCGAG CCCAGCTCTT CATCCCACTC
    AACACCAGCT GCCTGCTCAA GGAGGGCAGT GAGCACCTGT TTGATAGCTT CGAGCCAGAA
    ACATACTGGG ATCGAATGCA CCTTTTCCAG GAAGCCATTG CACACAGGTT TGGATTTGTG
    CAAGATAAAT ATTCCGCCTC TGCTTTTAAC TTCCCTGCTG AGAACAAGCC TCAGTATATC
    CATGTTACAG GAACAGTGTT CCTGCAGCTG CCTTACTCCA GGCGCAAGTT CTCCGGGCAG
    CAGCGGCGGC GGCGGAACTC CACCAGTTCC ACCAACCAGA ACTTGTTCTG TGAGGAGCGT
    GTTGGCTACA ACTGGGCCTA CAACACCATG CTGACCAAGA CATGGCGTTC TAGTGCCACG
    GGGGATGAGA AGTTTGCTGA TCGGCTGCTC AGGGATTTCA CGGACTTCTG CATCAACCGC
    GACAACCGGC TGGTCACATT CTGGATGAGC TGCCTGGAGA AGATGCATGC CAGTGCCCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26565
    Clone ID Related Accession (Same CDS sequence) XM_014108001.1 , XM_014108001.2
    Accession Version XM_014108001.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4743bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108001.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGTGTCT TTGGACCAGA CGGCCACCCC GGTGTTAGAC
    AGCACCAGTT TAGGGGTAAG CACAGGCCAG TCTGTGGACA GAGGCAACAG CCAGGCCTTT
    GGGAACTCCC AGAACTCAGG GGAACAGAGC TTCCCCTCTG CAAACTCCAG TGATAGCAGC
    TCTCAGCAGC TGGCAGCAAG CTCTTTAACC TCATCCTCCA CCTTGACAGA GATCCTGGAA
    GCCATGAAGC ACCCCTCGAC AGGGGTCCAG CTGCTCTCAG AACAGAAGGG CCTCTCACCA
    TGCTGCTTCA TCAGTGCGGA GGTGGTGCAC TGGCTGGTGA ATAACGTGGA GGGTGTCCAG
    ACCCAGGCGA TGGCTATCGA CATCATGCAG AAAATGCTGG AAGAGCAACT CATCACACAC
    GCATCTGGTG AAGTCTGGCG GACCTTCATC TATGGCTTCT ATTTCTACAA GATAGTCATG
    GACAAAGAAC CTGACCGAGT GGCCATGCAG CAGCCCACCA CCTGGCACAC GACGGCGGGG
    GAGGACTTCG CCAGCTTCCA GCGCAAGTGG TTTGAGGTGG CCTTTGTGGC CGAAGAGCTG
    TTGCACTCCG AGATCCCTGC TTTCCTCTTG CCCTGGCTGC CCAGCCGACC AGCCTCCTAT
    GCCAGCAGGC ACAGCTCCTT CAGCCGCAGC TTTGGAGGGC GGAGCCAGGC AGCTGCACTT
    TTAGCTGCCA CTGTCCCAGA GCAGAGGACT GTGACCCTGG ATGTGGACGT GAACAACCGC
    ACAGACCGGC TGGAGTGGTG CAGCTGTTAT TATCATGGCA ATTTCTCCCT GAATGCAGCT
    TTTGAGATCA AGCTCCACTG GATGGCTGTG ACTGCTGCGG TCCTCTTCGA GATGGTCCAG
    GGTTGGCATC GCAAAGCCAC TTCCTGCGGC TTTTTATTGG TCCCTGTTCT GGAGGGTCCC
    TTTGCGCTGC CCAGTTACCT GTATGGCGAT CCACTTCGAG CCCAGCTCTT CATCCCACTC
    AACACCAGCT GCCTGCTCAA GGAGGGCAGT GAGCACCTGT TTGATAGCTT CGAGCCAGAA
    ACATACTGGG ATCGAATGCA CCTTTTCCAG GAAGCCATTG CACACAGGTT TGGATTTGTG
    CAAGATAAAT ATTCCGCCTC TGCTTTTAAC TTCCCTGCTG AGAACAAGCC TCAGTATATC
    CATGTTACAG GAACAGTGTT CCTGCAGCTG CCTTACTCCA GGCGCAAGTT CTCCGGGCAG
    CAGCGGCGGC GGCGGAACTC CACCAGTTCC ACCAACCAGA ACTTGTTCTG TGAGGAGCGT
    GTTGGCTACA ACTGGGCCTA CAACACCATG CTGACCAAGA CATGGCGTTC TAGTGCCACG
    GGGGATGAGA AGTTTGCTGA TCGGCTGCTC AGGGATTTCA CGGACTTCTG CATCAACCGC
    GACAACCGGC TGGTCACATT CTGGATGAGC TGCCTGGAGA AGATGCATGC CAGTGCCCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963476.1
    CDS216..4958
    Translation

    Target ORF information:

    RefSeq Version XM_014108001.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108001.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGTGTCT TTGGACCAGA CGGCCACCCC GGTGTTAGAC
    AGCACCAGTT TAGGGGTAAG CACAGGCCAG TCTGTGGACA GAGGCAACAG CCAGGCCTTT
    GGGAACTCCC AGAACTCAGG GGAACAGAGC TTCCCCTCTG CAAACTCCAG TGATAGCAGC
    TCTCAGCAGC TGGCAGCAAG CTCTTTAACC TCATCCTCCA CCTTGACAGA GATCCTGGAA
    GCCATGAAGC ACCCCTCGAC AGGGGTCCAG CTGCTCTCAG AACAGAAGGG CCTCTCACCA
    TGCTGCTTCA TCAGTGCGGA GGTGGTGCAC TGGCTGGTGA ATAACGTGGA GGGTGTCCAG
    ACCCAGGCGA TGGCTATCGA CATCATGCAG AAAATGCTGG AAGAGCAACT CATCACACAC
    GCATCTGGTG AAGTCTGGCG GACCTTCATC TATGGCTTCT ATTTCTACAA GATAGTCATG
    GACAAAGAAC CTGACCGAGT GGCCATGCAG CAGCCCACCA CCTGGCACAC GACGGCGGGG
    GAGGACTTCG CCAGCTTCCA GCGCAAGTGG TTTGAGGTGG CCTTTGTGGC CGAAGAGCTG
    TTGCACTCCG AGATCCCTGC TTTCCTCTTG CCCTGGCTGC CCAGCCGACC AGCCTCCTAT
    GCCAGCAGGC ACAGCTCCTT CAGCCGCAGC TTTGGAGGGC GGAGCCAGGC AGCTGCACTT
    TTAGCTGCCA CTGTCCCAGA GCAGAGGACT GTGACCCTGG ATGTGGACGT GAACAACCGC
    ACAGACCGGC TGGAGTGGTG CAGCTGTTAT TATCATGGCA ATTTCTCCCT GAATGCAGCT
    TTTGAGATCA AGCTCCACTG GATGGCTGTG ACTGCTGCGG TCCTCTTCGA GATGGTCCAG
    GGTTGGCATC GCAAAGCCAC TTCCTGCGGC TTTTTATTGG TCCCTGTTCT GGAGGGTCCC
    TTTGCGCTGC CCAGTTACCT GTATGGCGAT CCACTTCGAG CCCAGCTCTT CATCCCACTC
    AACACCAGCT GCCTGCTCAA GGAGGGCAGT GAGCACCTGT TTGATAGCTT CGAGCCAGAA
    ACATACTGGG ATCGAATGCA CCTTTTCCAG GAAGCCATTG CACACAGGTT TGGATTTGTG
    CAAGATAAAT ATTCCGCCTC TGCTTTTAAC TTCCCTGCTG AGAACAAGCC TCAGTATATC
    CATGTTACAG GAACAGTGTT CCTGCAGCTG CCTTACTCCA GGCGCAAGTT CTCCGGGCAG
    CAGCGGCGGC GGCGGAACTC CACCAGTTCC ACCAACCAGA ACTTGTTCTG TGAGGAGCGT
    GTTGGCTACA ACTGGGCCTA CAACACCATG CTGACCAAGA CATGGCGTTC TAGTGCCACG
    GGGGATGAGA AGTTTGCTGA TCGGCTGCTC AGGGATTTCA CGGACTTCTG CATCAACCGC
    GACAACCGGC TGGTCACATT CTGGATGAGC TGCCTGGAGA AGATGCATGC CAGTGCCCCG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26566
    Clone ID Related Accession (Same CDS sequence) XM_014108002.1 , XM_014108002.2
    Accession Version XM_014108002.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGTG
    TCTTTGGACC AGACGGCCAC CCCGGTGTTA GACAGCACCA GTTTAGGGGT AAGCACAGGC
    CAGTCTGTGG ACAGAGGCAA CAGCCAGGCC TTTGGGAACT CCCAGAACTC AGGGGAACAG
    AGCTTCCCCT CTGCAAACTC CAGTGATAGC AGCTCTCAGC AGCTGGCAGC AAGCTCTTTA
    ACCTCATCCT CCACCTTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGGGTC
    CAGCTGCTCT CAGAACAGAA GGGCCTCTCA CCATGCTGCT TCATCAGTGC GGAGGTGGTG
    CACTGGCTGG TGAATAACGT GGAGGGTGTC CAGACCCAGG CGATGGCTAT CGACATCATG
    CAGAAAATGC TGGAAGAGCA ACTCATCACA CACGCATCTG GTGAAGTCTG GCGGACCTTC
    ATCTATGGCT TCTATTTCTA CAAGATAGTC ATGGACAAAG AACCTGACCG AGTGGCCATG
    CAGCAGCCCA CCACCTGGCA CACGACGGCG GGGGAGGACT TCGCCAGCTT CCAGCGCAAG
    TGGTTTGAGG TGGCCTTTGT GGCCGAAGAG CTGTTGCACT CCGAGATCCC TGCTTTCCTC
    TTGCCCTGGC TGCCCAGCCG ACCAGCCTCC TATGCCAGCA GGCACAGCTC CTTCAGCCGC
    AGCTTTGGAG GGCGGAGCCA GGCAGCTGCA CTTTTAGCTG CCACTGTCCC AGAGCAGAGG
    ACTGTGACCC TGGATGTGGA CGTGAACAAC CGCACAGACC GGCTGGAGTG GTGCAGCTGT
    TATTATCATG GCAATTTCTC CCTGAATGCA GCTTTTGAGA TCAAGCTCCA CTGGATGGCT
    GTGACTGCTG CGGTCCTCTT CGAGATGGTC CAGGGTTGGC ATCGCAAAGC CACTTCCTGC
    GGCTTTTTAT TGGTCCCTGT TCTGGAGGGT CCCTTTGCGC TGCCCAGTTA CCTGTATGGC
    GATCCACTTC GAGCCCAGCT CTTCATCCCA CTCAACACCA GCTGCCTGCT CAAGGAGGGC
    AGTGAGCACC TGTTTGATAG CTTCGAGCCA GAAACATACT GGGATCGAAT GCACCTTTTC
    CAGGAAGCCA TTGCACACAG GTTTGGATTT GTGCAAGATA AATATTCCGC CTCTGCTTTT
    AACTTCCCTG CTGAGAACAA GCCTCAGTAT ATCCATGTTA CAGGAACAGT GTTCCTGCAG
    CTGCCTTACT CCAGGCGCAA GTTCTCCGGG CAGCAGCGGC GGCGGCGGAA CTCCACCAGT
    TCCACCAACC AGAACTTGTT CTGTGAGGAG CGTGTTGGCT ACAACTGGGC CTACAACACC
    ATGCTGACCA AGACATGGCG TTCTAGTGCC ACGGGGGATG AGAAGTTTGC TGATCGGCTG
    CTCAGGGATT TCACGGACTT CTGCATCAAC CGCGACAACC GGCTGGTCAC ATTCTGGATG
    AGCTGCCTGG AGAAGATGCA TGCCAGTGCC CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963477.1
    CDS228..4943
    Translation

    Target ORF information:

    RefSeq Version XM_014108002.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108002.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGTG
    TCTTTGGACC AGACGGCCAC CCCGGTGTTA GACAGCACCA GTTTAGGGGT AAGCACAGGC
    CAGTCTGTGG ACAGAGGCAA CAGCCAGGCC TTTGGGAACT CCCAGAACTC AGGGGAACAG
    AGCTTCCCCT CTGCAAACTC CAGTGATAGC AGCTCTCAGC AGCTGGCAGC AAGCTCTTTA
    ACCTCATCCT CCACCTTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGGGTC
    CAGCTGCTCT CAGAACAGAA GGGCCTCTCA CCATGCTGCT TCATCAGTGC GGAGGTGGTG
    CACTGGCTGG TGAATAACGT GGAGGGTGTC CAGACCCAGG CGATGGCTAT CGACATCATG
    CAGAAAATGC TGGAAGAGCA ACTCATCACA CACGCATCTG GTGAAGTCTG GCGGACCTTC
    ATCTATGGCT TCTATTTCTA CAAGATAGTC ATGGACAAAG AACCTGACCG AGTGGCCATG
    CAGCAGCCCA CCACCTGGCA CACGACGGCG GGGGAGGACT TCGCCAGCTT CCAGCGCAAG
    TGGTTTGAGG TGGCCTTTGT GGCCGAAGAG CTGTTGCACT CCGAGATCCC TGCTTTCCTC
    TTGCCCTGGC TGCCCAGCCG ACCAGCCTCC TATGCCAGCA GGCACAGCTC CTTCAGCCGC
    AGCTTTGGAG GGCGGAGCCA GGCAGCTGCA CTTTTAGCTG CCACTGTCCC AGAGCAGAGG
    ACTGTGACCC TGGATGTGGA CGTGAACAAC CGCACAGACC GGCTGGAGTG GTGCAGCTGT
    TATTATCATG GCAATTTCTC CCTGAATGCA GCTTTTGAGA TCAAGCTCCA CTGGATGGCT
    GTGACTGCTG CGGTCCTCTT CGAGATGGTC CAGGGTTGGC ATCGCAAAGC CACTTCCTGC
    GGCTTTTTAT TGGTCCCTGT TCTGGAGGGT CCCTTTGCGC TGCCCAGTTA CCTGTATGGC
    GATCCACTTC GAGCCCAGCT CTTCATCCCA CTCAACACCA GCTGCCTGCT CAAGGAGGGC
    AGTGAGCACC TGTTTGATAG CTTCGAGCCA GAAACATACT GGGATCGAAT GCACCTTTTC
    CAGGAAGCCA TTGCACACAG GTTTGGATTT GTGCAAGATA AATATTCCGC CTCTGCTTTT
    AACTTCCCTG CTGAGAACAA GCCTCAGTAT ATCCATGTTA CAGGAACAGT GTTCCTGCAG
    CTGCCTTACT CCAGGCGCAA GTTCTCCGGG CAGCAGCGGC GGCGGCGGAA CTCCACCAGT
    TCCACCAACC AGAACTTGTT CTGTGAGGAG CGTGTTGGCT ACAACTGGGC CTACAACACC
    ATGCTGACCA AGACATGGCG TTCTAGTGCC ACGGGGGATG AGAAGTTTGC TGATCGGCTG
    CTCAGGGATT TCACGGACTT CTGCATCAAC CGCGACAACC GGCTGGTCAC ATTCTGGATG
    AGCTGCCTGG AGAAGATGCA TGCCAGTGCC CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26566
    Clone ID Related Accession (Same CDS sequence) XM_014108002.1 , XM_014108002.2
    Accession Version XM_014108002.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4716bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108002.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGTG
    TCTTTGGACC AGACGGCCAC CCCGGTGTTA GACAGCACCA GTTTAGGGGT AAGCACAGGC
    CAGTCTGTGG ACAGAGGCAA CAGCCAGGCC TTTGGGAACT CCCAGAACTC AGGGGAACAG
    AGCTTCCCCT CTGCAAACTC CAGTGATAGC AGCTCTCAGC AGCTGGCAGC AAGCTCTTTA
    ACCTCATCCT CCACCTTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGGGTC
    CAGCTGCTCT CAGAACAGAA GGGCCTCTCA CCATGCTGCT TCATCAGTGC GGAGGTGGTG
    CACTGGCTGG TGAATAACGT GGAGGGTGTC CAGACCCAGG CGATGGCTAT CGACATCATG
    CAGAAAATGC TGGAAGAGCA ACTCATCACA CACGCATCTG GTGAAGTCTG GCGGACCTTC
    ATCTATGGCT TCTATTTCTA CAAGATAGTC ATGGACAAAG AACCTGACCG AGTGGCCATG
    CAGCAGCCCA CCACCTGGCA CACGACGGCG GGGGAGGACT TCGCCAGCTT CCAGCGCAAG
    TGGTTTGAGG TGGCCTTTGT GGCCGAAGAG CTGTTGCACT CCGAGATCCC TGCTTTCCTC
    TTGCCCTGGC TGCCCAGCCG ACCAGCCTCC TATGCCAGCA GGCACAGCTC CTTCAGCCGC
    AGCTTTGGAG GGCGGAGCCA GGCAGCTGCA CTTTTAGCTG CCACTGTCCC AGAGCAGAGG
    ACTGTGACCC TGGATGTGGA CGTGAACAAC CGCACAGACC GGCTGGAGTG GTGCAGCTGT
    TATTATCATG GCAATTTCTC CCTGAATGCA GCTTTTGAGA TCAAGCTCCA CTGGATGGCT
    GTGACTGCTG CGGTCCTCTT CGAGATGGTC CAGGGTTGGC ATCGCAAAGC CACTTCCTGC
    GGCTTTTTAT TGGTCCCTGT TCTGGAGGGT CCCTTTGCGC TGCCCAGTTA CCTGTATGGC
    GATCCACTTC GAGCCCAGCT CTTCATCCCA CTCAACACCA GCTGCCTGCT CAAGGAGGGC
    AGTGAGCACC TGTTTGATAG CTTCGAGCCA GAAACATACT GGGATCGAAT GCACCTTTTC
    CAGGAAGCCA TTGCACACAG GTTTGGATTT GTGCAAGATA AATATTCCGC CTCTGCTTTT
    AACTTCCCTG CTGAGAACAA GCCTCAGTAT ATCCATGTTA CAGGAACAGT GTTCCTGCAG
    CTGCCTTACT CCAGGCGCAA GTTCTCCGGG CAGCAGCGGC GGCGGCGGAA CTCCACCAGT
    TCCACCAACC AGAACTTGTT CTGTGAGGAG CGTGTTGGCT ACAACTGGGC CTACAACACC
    ATGCTGACCA AGACATGGCG TTCTAGTGCC ACGGGGGATG AGAAGTTTGC TGATCGGCTG
    CTCAGGGATT TCACGGACTT CTGCATCAAC CGCGACAACC GGCTGGTCAC ATTCTGGATG
    AGCTGCCTGG AGAAGATGCA TGCCAGTGCC CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963477.1
    CDS216..4931
    Translation

    Target ORF information:

    RefSeq Version XM_014108002.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108002.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TGCCAGGCTT CTGTTGCACA GTTGGAGTGG ACTGGAAGTC TCTCACTACT
    CCGGCGTGCC TCCCCCTCAC CACCGACTAC TTCCCCGACC GCCAGGGCCT GCAGAACGAC
    TACACAGAGG GCTGTTACGA TCTCCTTCCA GAAGCAGATA TTGACAGGAG GGATGAGGAG
    GGTGTGCAGA TGACGGCCCA GCAGGTGTTT GAAGAGTTCA TCTGCCAGCG TCTCATGCAG
    GGCTACCAAA TCATAGTGCA GCCCAAGGCA CAAAAATCCA ACCCCGTCGT CCCACCCCCA
    CTGAGCAGCA GCCCGCTCTA TAGTCGAGGC CTTGTGTCCC GAAACCGCCC TGAGGAGGAG
    GACCAATACT GGCTGAGTAT GGGCAGAACG TTCCACAAAG TGACGCTGAA AGATAAGATG
    ATCACAGTGA CACGTTACCT TCCCAAGTAC CCTTATGAAT CTGCCCAGAT CCACTACACC
    TACAGTCTCT GCCCTTCCCA CTCAGACTCG GAGTTCGTCT CTTGTTGGGT GGAATTCTCC
    CATGAGCGGC TGGAGGAGTA CAAGTGGAAT TACTTAGATC AGTATATCTG TTCTGCTGGC
    TCTGAGGACT TCAGCTTAAT TGAGTCTCTG AAGTTCTGGA GGACCCGCTT CCTGCTGCTG
    CCAGCCTGTG TCACTGCCAC TAAGCGCATC ACAGAAGGGG AGGCCCACTG CGACATCTAT
    GGGGACAGGC CTCGGGCAGA CGAGGAGGAA TGGCAGCTCT TGGATGGTTT TATCCGCTTC
    GTGGAGGGTT TGAACCGGAT CCGCAGGCGA CATCGCTCAG ATCGCATGAT GCGGAAAGGG
    ACCGCCATGA AAGGTTTGCA GATGACTGGG CCCATTGCCA CACACTCCCT TGAGTCGACC
    GGCCCCCCAG TTGGGAAGAA GGGAACCTCA GCTCTGTCCG CCCTGTTGGA GATGGAGGCC
    AGTCAGAAGT GCCTAGGAGA ACAGCAGGCA GCTGTGCACG GTGGCAAGAG CGCCTCCCAG
    CCAGCTGAGA GCAGCAGCGT TGCCATGACT CCCACCTACG TGGACAGCCC ACGAAAGGTG
    TCTTTGGACC AGACGGCCAC CCCGGTGTTA GACAGCACCA GTTTAGGGGT AAGCACAGGC
    CAGTCTGTGG ACAGAGGCAA CAGCCAGGCC TTTGGGAACT CCCAGAACTC AGGGGAACAG
    AGCTTCCCCT CTGCAAACTC CAGTGATAGC AGCTCTCAGC AGCTGGCAGC AAGCTCTTTA
    ACCTCATCCT CCACCTTGAC AGAGATCCTG GAAGCCATGA AGCACCCCTC GACAGGGGTC
    CAGCTGCTCT CAGAACAGAA GGGCCTCTCA CCATGCTGCT TCATCAGTGC GGAGGTGGTG
    CACTGGCTGG TGAATAACGT GGAGGGTGTC CAGACCCAGG CGATGGCTAT CGACATCATG
    CAGAAAATGC TGGAAGAGCA ACTCATCACA CACGCATCTG GTGAAGTCTG GCGGACCTTC
    ATCTATGGCT TCTATTTCTA CAAGATAGTC ATGGACAAAG AACCTGACCG AGTGGCCATG
    CAGCAGCCCA CCACCTGGCA CACGACGGCG GGGGAGGACT TCGCCAGCTT CCAGCGCAAG
    TGGTTTGAGG TGGCCTTTGT GGCCGAAGAG CTGTTGCACT CCGAGATCCC TGCTTTCCTC
    TTGCCCTGGC TGCCCAGCCG ACCAGCCTCC TATGCCAGCA GGCACAGCTC CTTCAGCCGC
    AGCTTTGGAG GGCGGAGCCA GGCAGCTGCA CTTTTAGCTG CCACTGTCCC AGAGCAGAGG
    ACTGTGACCC TGGATGTGGA CGTGAACAAC CGCACAGACC GGCTGGAGTG GTGCAGCTGT
    TATTATCATG GCAATTTCTC CCTGAATGCA GCTTTTGAGA TCAAGCTCCA CTGGATGGCT
    GTGACTGCTG CGGTCCTCTT CGAGATGGTC CAGGGTTGGC ATCGCAAAGC CACTTCCTGC
    GGCTTTTTAT TGGTCCCTGT TCTGGAGGGT CCCTTTGCGC TGCCCAGTTA CCTGTATGGC
    GATCCACTTC GAGCCCAGCT CTTCATCCCA CTCAACACCA GCTGCCTGCT CAAGGAGGGC
    AGTGAGCACC TGTTTGATAG CTTCGAGCCA GAAACATACT GGGATCGAAT GCACCTTTTC
    CAGGAAGCCA TTGCACACAG GTTTGGATTT GTGCAAGATA AATATTCCGC CTCTGCTTTT
    AACTTCCCTG CTGAGAACAA GCCTCAGTAT ATCCATGTTA CAGGAACAGT GTTCCTGCAG
    CTGCCTTACT CCAGGCGCAA GTTCTCCGGG CAGCAGCGGC GGCGGCGGAA CTCCACCAGT
    TCCACCAACC AGAACTTGTT CTGTGAGGAG CGTGTTGGCT ACAACTGGGC CTACAACACC
    ATGCTGACCA AGACATGGCG TTCTAGTGCC ACGGGGGATG AGAAGTTTGC TGATCGGCTG
    CTCAGGGATT TCACGGACTT CTGCATCAAC CGCGACAACC GGCTGGTCAC ATTCTGGATG
    AGCTGCCTGG AGAAGATGCA TGCCAGTGCC CCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26567
    Clone ID Related Accession (Same CDS sequence) XM_014108003.2 , XM_014108003.1
    Accession Version XM_014108003.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108003.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC
    AGCCCTCGCA CAGCGTCATC CGCCTTCTAC CCACAGGTGT CTTTGGACCA GACGGCCACC
    CCGGTGTTAG ACAGCACCAG TTTAGGGGTA AGCACAGGCC AGTCTGTGGA CAGAGGCAAC
    AGCCAGGCCT TTGGGAACTC CCAGAACTCA GGGGAACAGA GCTTCCCCTC TGCAAACTCC
    AGTGATAGCA GCTCTCAGCA GCTGGCAGCA AGCTCTTTAA CCTCATCCTC CACCTTGACA
    GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGGGTCC AGCTGCTCTC AGAACAGAAG
    GGCCTCTCAC CATGCTGCTT CATCAGTGCG GAGGTGGTGC ACTGGCTGGT GAATAACGTG
    GAGGGTGTCC AGACCCAGGC GATGGCTATC GACATCATGC AGAAAATGCT GGAAGAGCAA
    CTCATCACAC ACGCATCTGG TGAAGTCTGG CGGACCTTCA TCTATGGCTT CTATTTCTAC
    AAGATAGTCA TGGACAAAGA ACCTGACCGA GTGGCCATGC AGCAGCCCAC CACCTGGCAC
    ACGACGGCGG GGGAGGACTT CGCCAGCTTC CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG
    GCCGAAGAGC TGTTGCACTC CGAGATCCCT GCTTTCCTCT TGCCCTGGCT GCCCAGCCGA
    CCAGCCTCCT ATGCCAGCAG GCACAGCTCC TTCAGCCGCA GCTTTGGAGG GCGGAGCCAG
    GCAGCTGCAC TTTTAGCTGC CACTGTCCCA GAGCAGAGGA CTGTGACCCT GGATGTGGAC
    GTGAACAACC GCACAGACCG GCTGGAGTGG TGCAGCTGTT ATTATCATGG CAATTTCTCC
    CTGAATGCAG CTTTTGAGAT CAAGCTCCAC TGGATGGCTG TGACTGCTGC GGTCCTCTTC
    GAGATGGTCC AGGGTTGGCA TCGCAAAGCC ACTTCCTGCG GCTTTTTATT GGTCCCTGTT
    CTGGAGGGTC CCTTTGCGCT GCCCAGTTAC CTGTATGGCG ATCCACTTCG AGCCCAGCTC
    TTCATCCCAC TCAACACCAG CTGCCTGCTC AAGGAGGGCA GTGAGCACCT GTTTGATAGC
    TTCGAGCCAG AAACATACTG GGATCGAATG CACCTTTTCC AGGAAGCCAT TGCACACAGG
    TTTGGATTTG TGCAAGATAA ATATTCCGCC TCTGCTTTTA ACTTCCCTGC TGAGAACAAG
    CCTCAGTATA TCCATGTTAC AGGAACAGTG TTCCTGCAGC TGCCTTACTC CAGGCGCAAG
    TTCTCCGGGC AGCAGCGGCG GCGGCGGAAC TCCACCAGTT CCACCAACCA GAACTTGTTC
    TGTGAGGAGC GTGTTGGCTA CAACTGGGCC TACAACACCA TGCTGACCAA GACATGGCGT
    TCTAGTGCCA CGGGGGATGA GAAGTTTGCT GATCGGCTGC TCAGGGATTT CACGGACTTC
    TGCATCAACC GCGACAACCG GCTGGTCACA TTCTGGATGA GCTGCCTGGA GAAGATGCAT
    GCCAGTGCCC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963478.1
    CDS216..4790
    Translation

    Target ORF information:

    RefSeq Version XM_014108003.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108003.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC
    AGCCCTCGCA CAGCGTCATC CGCCTTCTAC CCACAGGTGT CTTTGGACCA GACGGCCACC
    CCGGTGTTAG ACAGCACCAG TTTAGGGGTA AGCACAGGCC AGTCTGTGGA CAGAGGCAAC
    AGCCAGGCCT TTGGGAACTC CCAGAACTCA GGGGAACAGA GCTTCCCCTC TGCAAACTCC
    AGTGATAGCA GCTCTCAGCA GCTGGCAGCA AGCTCTTTAA CCTCATCCTC CACCTTGACA
    GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGGGTCC AGCTGCTCTC AGAACAGAAG
    GGCCTCTCAC CATGCTGCTT CATCAGTGCG GAGGTGGTGC ACTGGCTGGT GAATAACGTG
    GAGGGTGTCC AGACCCAGGC GATGGCTATC GACATCATGC AGAAAATGCT GGAAGAGCAA
    CTCATCACAC ACGCATCTGG TGAAGTCTGG CGGACCTTCA TCTATGGCTT CTATTTCTAC
    AAGATAGTCA TGGACAAAGA ACCTGACCGA GTGGCCATGC AGCAGCCCAC CACCTGGCAC
    ACGACGGCGG GGGAGGACTT CGCCAGCTTC CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG
    GCCGAAGAGC TGTTGCACTC CGAGATCCCT GCTTTCCTCT TGCCCTGGCT GCCCAGCCGA
    CCAGCCTCCT ATGCCAGCAG GCACAGCTCC TTCAGCCGCA GCTTTGGAGG GCGGAGCCAG
    GCAGCTGCAC TTTTAGCTGC CACTGTCCCA GAGCAGAGGA CTGTGACCCT GGATGTGGAC
    GTGAACAACC GCACAGACCG GCTGGAGTGG TGCAGCTGTT ATTATCATGG CAATTTCTCC
    CTGAATGCAG CTTTTGAGAT CAAGCTCCAC TGGATGGCTG TGACTGCTGC GGTCCTCTTC
    GAGATGGTCC AGGGTTGGCA TCGCAAAGCC ACTTCCTGCG GCTTTTTATT GGTCCCTGTT
    CTGGAGGGTC CCTTTGCGCT GCCCAGTTAC CTGTATGGCG ATCCACTTCG AGCCCAGCTC
    TTCATCCCAC TCAACACCAG CTGCCTGCTC AAGGAGGGCA GTGAGCACCT GTTTGATAGC
    TTCGAGCCAG AAACATACTG GGATCGAATG CACCTTTTCC AGGAAGCCAT TGCACACAGG
    TTTGGATTTG TGCAAGATAA ATATTCCGCC TCTGCTTTTA ACTTCCCTGC TGAGAACAAG
    CCTCAGTATA TCCATGTTAC AGGAACAGTG TTCCTGCAGC TGCCTTACTC CAGGCGCAAG
    TTCTCCGGGC AGCAGCGGCG GCGGCGGAAC TCCACCAGTT CCACCAACCA GAACTTGTTC
    TGTGAGGAGC GTGTTGGCTA CAACTGGGCC TACAACACCA TGCTGACCAA GACATGGCGT
    TCTAGTGCCA CGGGGGATGA GAAGTTTGCT GATCGGCTGC TCAGGGATTT CACGGACTTC
    TGCATCAACC GCGACAACCG GCTGGTCACA TTCTGGATGA GCTGCCTGGA GAAGATGCAT
    GCCAGTGCCC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26567
    Clone ID Related Accession (Same CDS sequence) XM_014108003.2 , XM_014108003.1
    Accession Version XM_014108003.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4575bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC
    AGCCCTCGCA CAGCGTCATC CGCCTTCTAC CCACAGGTGT CTTTGGACCA GACGGCCACC
    CCGGTGTTAG ACAGCACCAG TTTAGGGGTA AGCACAGGCC AGTCTGTGGA CAGAGGCAAC
    AGCCAGGCCT TTGGGAACTC CCAGAACTCA GGGGAACAGA GCTTCCCCTC TGCAAACTCC
    AGTGATAGCA GCTCTCAGCA GCTGGCAGCA AGCTCTTTAA CCTCATCCTC CACCTTGACA
    GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGGGTCC AGCTGCTCTC AGAACAGAAG
    GGCCTCTCAC CATGCTGCTT CATCAGTGCG GAGGTGGTGC ACTGGCTGGT GAATAACGTG
    GAGGGTGTCC AGACCCAGGC GATGGCTATC GACATCATGC AGAAAATGCT GGAAGAGCAA
    CTCATCACAC ACGCATCTGG TGAAGTCTGG CGGACCTTCA TCTATGGCTT CTATTTCTAC
    AAGATAGTCA TGGACAAAGA ACCTGACCGA GTGGCCATGC AGCAGCCCAC CACCTGGCAC
    ACGACGGCGG GGGAGGACTT CGCCAGCTTC CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG
    GCCGAAGAGC TGTTGCACTC CGAGATCCCT GCTTTCCTCT TGCCCTGGCT GCCCAGCCGA
    CCAGCCTCCT ATGCCAGCAG GCACAGCTCC TTCAGCCGCA GCTTTGGAGG GCGGAGCCAG
    GCAGCTGCAC TTTTAGCTGC CACTGTCCCA GAGCAGAGGA CTGTGACCCT GGATGTGGAC
    GTGAACAACC GCACAGACCG GCTGGAGTGG TGCAGCTGTT ATTATCATGG CAATTTCTCC
    CTGAATGCAG CTTTTGAGAT CAAGCTCCAC TGGATGGCTG TGACTGCTGC GGTCCTCTTC
    GAGATGGTCC AGGGTTGGCA TCGCAAAGCC ACTTCCTGCG GCTTTTTATT GGTCCCTGTT
    CTGGAGGGTC CCTTTGCGCT GCCCAGTTAC CTGTATGGCG ATCCACTTCG AGCCCAGCTC
    TTCATCCCAC TCAACACCAG CTGCCTGCTC AAGGAGGGCA GTGAGCACCT GTTTGATAGC
    TTCGAGCCAG AAACATACTG GGATCGAATG CACCTTTTCC AGGAAGCCAT TGCACACAGG
    TTTGGATTTG TGCAAGATAA ATATTCCGCC TCTGCTTTTA ACTTCCCTGC TGAGAACAAG
    CCTCAGTATA TCCATGTTAC AGGAACAGTG TTCCTGCAGC TGCCTTACTC CAGGCGCAAG
    TTCTCCGGGC AGCAGCGGCG GCGGCGGAAC TCCACCAGTT CCACCAACCA GAACTTGTTC
    TGTGAGGAGC GTGTTGGCTA CAACTGGGCC TACAACACCA TGCTGACCAA GACATGGCGT
    TCTAGTGCCA CGGGGGATGA GAAGTTTGCT GATCGGCTGC TCAGGGATTT CACGGACTTC
    TGCATCAACC GCGACAACCG GCTGGTCACA TTCTGGATGA GCTGCCTGGA GAAGATGCAT
    GCCAGTGCCC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963478.1
    CDS228..4802
    Translation

    Target ORF information:

    RefSeq Version XM_014108003.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108003.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GACGGGGCCT TCTTTATGGA GTTTGTCCGC
    AGCCCTCGCA CAGCGTCATC CGCCTTCTAC CCACAGGTGT CTTTGGACCA GACGGCCACC
    CCGGTGTTAG ACAGCACCAG TTTAGGGGTA AGCACAGGCC AGTCTGTGGA CAGAGGCAAC
    AGCCAGGCCT TTGGGAACTC CCAGAACTCA GGGGAACAGA GCTTCCCCTC TGCAAACTCC
    AGTGATAGCA GCTCTCAGCA GCTGGCAGCA AGCTCTTTAA CCTCATCCTC CACCTTGACA
    GAGATCCTGG AAGCCATGAA GCACCCCTCG ACAGGGGTCC AGCTGCTCTC AGAACAGAAG
    GGCCTCTCAC CATGCTGCTT CATCAGTGCG GAGGTGGTGC ACTGGCTGGT GAATAACGTG
    GAGGGTGTCC AGACCCAGGC GATGGCTATC GACATCATGC AGAAAATGCT GGAAGAGCAA
    CTCATCACAC ACGCATCTGG TGAAGTCTGG CGGACCTTCA TCTATGGCTT CTATTTCTAC
    AAGATAGTCA TGGACAAAGA ACCTGACCGA GTGGCCATGC AGCAGCCCAC CACCTGGCAC
    ACGACGGCGG GGGAGGACTT CGCCAGCTTC CAGCGCAAGT GGTTTGAGGT GGCCTTTGTG
    GCCGAAGAGC TGTTGCACTC CGAGATCCCT GCTTTCCTCT TGCCCTGGCT GCCCAGCCGA
    CCAGCCTCCT ATGCCAGCAG GCACAGCTCC TTCAGCCGCA GCTTTGGAGG GCGGAGCCAG
    GCAGCTGCAC TTTTAGCTGC CACTGTCCCA GAGCAGAGGA CTGTGACCCT GGATGTGGAC
    GTGAACAACC GCACAGACCG GCTGGAGTGG TGCAGCTGTT ATTATCATGG CAATTTCTCC
    CTGAATGCAG CTTTTGAGAT CAAGCTCCAC TGGATGGCTG TGACTGCTGC GGTCCTCTTC
    GAGATGGTCC AGGGTTGGCA TCGCAAAGCC ACTTCCTGCG GCTTTTTATT GGTCCCTGTT
    CTGGAGGGTC CCTTTGCGCT GCCCAGTTAC CTGTATGGCG ATCCACTTCG AGCCCAGCTC
    TTCATCCCAC TCAACACCAG CTGCCTGCTC AAGGAGGGCA GTGAGCACCT GTTTGATAGC
    TTCGAGCCAG AAACATACTG GGATCGAATG CACCTTTTCC AGGAAGCCAT TGCACACAGG
    TTTGGATTTG TGCAAGATAA ATATTCCGCC TCTGCTTTTA ACTTCCCTGC TGAGAACAAG
    CCTCAGTATA TCCATGTTAC AGGAACAGTG TTCCTGCAGC TGCCTTACTC CAGGCGCAAG
    TTCTCCGGGC AGCAGCGGCG GCGGCGGAAC TCCACCAGTT CCACCAACCA GAACTTGTTC
    TGTGAGGAGC GTGTTGGCTA CAACTGGGCC TACAACACCA TGCTGACCAA GACATGGCGT
    TCTAGTGCCA CGGGGGATGA GAAGTTTGCT GATCGGCTGC TCAGGGATTT CACGGACTTC
    TGCATCAACC GCGACAACCG GCTGGTCACA TTCTGGATGA GCTGCCTGGA GAAGATGCAT
    GCCAGTGCCC CGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26568
    Clone ID Related Accession (Same CDS sequence) XM_014108004.2 , XM_014108004.1
    Accession Version XM_014108004.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108004.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATGCC AGGCTTCTGT TGCACAGTTG GAGTGGACTG GAAGTCTCTC
    ACTACTCCGG CGTGCCTCCC CCTCACCACC GACTACTTCC CCGACCGCCA GGGCCTGCAG
    AACGACTACA CAGAGGGCTG TTACGATCTC CTTCCAGAAG CAGATATTGA CAGGAGGGAT
    GAGGAGGGTG TGCAGATGAC GGCCCAGCAG GTGTTTGAAG AGTTCATCTG CCAGCGTCTC
    ATGCAGGGCT ACCAAATCAT AGTGCAGCCC AAGGCACAAA AATCCAACCC CGTCGTCCCA
    CCCCCACTGA GCAGCAGCCC GCTCTATAGT CGAGGCCTTG TGTCCCGAAA CCGCCCTGAG
    GAGGAGGACC AATACTGGCT GAGTATGGGC AGAACGTTCC ACAAAGTGAC GCTGAAAGAT
    AAGATGATCA CAGTGACACG TTACCTTCCC AAGTACCCTT ATGAATCTGC CCAGATCCAC
    TACACCTACA GTCTCTGCCC TTCCCACTCA GACTCGGAGT TCGTCTCTTG TTGGGTGGAA
    TTCTCCCATG AGCGGCTGGA GGAGTACAAG TGGAATTACT TAGATCAGTA TATCTGTTCT
    GCTGGCTCTG AGGACTTCAG CTTAATTGAG TCTCTGAAGT TCTGGAGGAC CCGCTTCCTG
    CTGCTGCCAG CCTGTGTCAC TGCCACTAAG CGCATCACAG AAGGGGAGGC CCACTGCGAC
    ATCTATGGGG ACAGGCCTCG GGCAGACGAG GAGGAATGGC AGCTCTTGGA TGGTTTTATC
    CGCTTCGTGG AGGGTTTGAA CCGGATCCGC AGGCGACATC GCTCAGATCG CATGATGCGG
    AAAGGGACCG CCATGAAAGG TTTGCAGATG ACTGGGCCCA TTGCCACACA CTCCCTTGAG
    TCGACCGGCC CCCCAGTTGG GAAGAAGGGA ACCTCAGCTC TGTCCGCCCT GTTGGAGATG
    GAGGCCAGTC AGAAGTGCCT AGGAGAACAG CAGGCAGCTG TGCACGGTGG CAAGAGCGCC
    TCCCAGCCAG CTGAGAGCAG CAGCGTTGCC ATGACTCCCA CCTACGTGGA CAGCCCACGA
    AAGGACGGGG CCTTCTTTAT GGAGTTTGTC CGCAGCCCTC GCACAGCGTC ATCCGCCTTC
    TACCCACAGG TGTCTTTGGA CCAGACGGCC ACCCCGGTGT TAGACAGCAC CAGTTTAGGG
    GTAAGCACAG GCCAGTCTGT GGACAGAGGC AACAGCCAGG CCTTTGGGAA CTCCCAGAAC
    TCAGGGGAAC AGAGCTTCCC CTCTGCAAAC TCCAGTGATA GCAGCTCTCA GCAGCTGGCA
    GCAAGCTCTT TAACCTCATC CTCCACCTTG ACAGAGATCC TGGAAGCCAT GAAGCACCCC
    TCGACAGGGG TCCAGCTGCT CTCAGAACAG AAGGGCCTCT CACCATGCTG CTTCATCAGT
    GCGGAGGTGG TGCACTGGCT GGTGAATAAC GTGGAGGGTG TCCAGACCCA GGCGATGGCT
    ATCGACATCA TGCAGAAAAT GCTGGAAGAG CAACTCATCA CACACGCATC TGGTGAAGTC
    TGGCGGACCT TCATCTATGG CTTCTATTTC TACAAGATAG TCATGGACAA AGAACCTGAC
    CGAGTGGCCA TGCAGCAGCC CACCACCTGG CACACGACGG CGGGGGAGGA CTTCGCCAGC
    TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCCGAAG AGCTGTTGCA CTCCGAGATC
    CCTGCTTTCC TCTTGCCCTG GCTGCCCAGC CGACCAGCCT CCTATGCCAG CAGGCACAGC
    TCCTTCAGCC GCAGCTTTGG AGGGCGGAGC CAGGCAGCTG CACTTTTAGC TGCCACTGTC
    CCAGAGCAGA GGACTGTGAC CCTGGATGTG GACGTGAACA ACCGCACAGA CCGGCTGGAG
    TGGTGCAGCT GTTATTATCA TGGCAATTTC TCCCTGAATG CAGCTTTTGA GATCAAGCTC
    CACTGGATGG CTGTGACTGC TGCGGTCCTC TTCGAGATGG TCCAGGGTTG GCATCGCAAA
    GCCACTTCCT GCGGCTTTTT ATTGGTCCCT GTTCTGGAGG GTCCCTTTGC GCTGCCCAGT
    TACCTGTATG GCGATCCACT TCGAGCCCAG CTCTTCATCC CACTCAACAC CAGCTGCCTG
    CTCAAGGAGG GCAGTGAGCA CCTGTTTGAT AGCTTCGAGC CAGAAACATA CTGGGATCGA
    ATGCACCTTT TCCAGGAAGC CATTGCACAC AGGTTTGGAT TTGTGCAAGA TAAATATTCC
    GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCATGT TACAGGAACA
    GTGTTCCTGC AGCTGCCTTA CTCCAGGCGC AAGTTCTCCG GGCAGCAGCG GCGGCGGCGG
    AACTCCACCA GTTCCACCAA CCAGAACTTG TTCTGTGAGG AGCGTGTTGG CTACAACTGG
    GCCTACAACA CCATGCTGAC CAAGACATGG CGTTCTAGTG CCACGGGGGA TGAGAAGTTT
    GCTGATCGGC TGCTCAGGGA TTTCACGGAC TTCTGCATCA ACCGCGACAA CCGGCTGGTC
    ACATTCTGGA TGAGCTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963479.1
    CDS216..4763
    Translation

    Target ORF information:

    RefSeq Version XM_014108004.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108004.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATGCC AGGCTTCTGT TGCACAGTTG GAGTGGACTG GAAGTCTCTC
    ACTACTCCGG CGTGCCTCCC CCTCACCACC GACTACTTCC CCGACCGCCA GGGCCTGCAG
    AACGACTACA CAGAGGGCTG TTACGATCTC CTTCCAGAAG CAGATATTGA CAGGAGGGAT
    GAGGAGGGTG TGCAGATGAC GGCCCAGCAG GTGTTTGAAG AGTTCATCTG CCAGCGTCTC
    ATGCAGGGCT ACCAAATCAT AGTGCAGCCC AAGGCACAAA AATCCAACCC CGTCGTCCCA
    CCCCCACTGA GCAGCAGCCC GCTCTATAGT CGAGGCCTTG TGTCCCGAAA CCGCCCTGAG
    GAGGAGGACC AATACTGGCT GAGTATGGGC AGAACGTTCC ACAAAGTGAC GCTGAAAGAT
    AAGATGATCA CAGTGACACG TTACCTTCCC AAGTACCCTT ATGAATCTGC CCAGATCCAC
    TACACCTACA GTCTCTGCCC TTCCCACTCA GACTCGGAGT TCGTCTCTTG TTGGGTGGAA
    TTCTCCCATG AGCGGCTGGA GGAGTACAAG TGGAATTACT TAGATCAGTA TATCTGTTCT
    GCTGGCTCTG AGGACTTCAG CTTAATTGAG TCTCTGAAGT TCTGGAGGAC CCGCTTCCTG
    CTGCTGCCAG CCTGTGTCAC TGCCACTAAG CGCATCACAG AAGGGGAGGC CCACTGCGAC
    ATCTATGGGG ACAGGCCTCG GGCAGACGAG GAGGAATGGC AGCTCTTGGA TGGTTTTATC
    CGCTTCGTGG AGGGTTTGAA CCGGATCCGC AGGCGACATC GCTCAGATCG CATGATGCGG
    AAAGGGACCG CCATGAAAGG TTTGCAGATG ACTGGGCCCA TTGCCACACA CTCCCTTGAG
    TCGACCGGCC CCCCAGTTGG GAAGAAGGGA ACCTCAGCTC TGTCCGCCCT GTTGGAGATG
    GAGGCCAGTC AGAAGTGCCT AGGAGAACAG CAGGCAGCTG TGCACGGTGG CAAGAGCGCC
    TCCCAGCCAG CTGAGAGCAG CAGCGTTGCC ATGACTCCCA CCTACGTGGA CAGCCCACGA
    AAGGACGGGG CCTTCTTTAT GGAGTTTGTC CGCAGCCCTC GCACAGCGTC ATCCGCCTTC
    TACCCACAGG TGTCTTTGGA CCAGACGGCC ACCCCGGTGT TAGACAGCAC CAGTTTAGGG
    GTAAGCACAG GCCAGTCTGT GGACAGAGGC AACAGCCAGG CCTTTGGGAA CTCCCAGAAC
    TCAGGGGAAC AGAGCTTCCC CTCTGCAAAC TCCAGTGATA GCAGCTCTCA GCAGCTGGCA
    GCAAGCTCTT TAACCTCATC CTCCACCTTG ACAGAGATCC TGGAAGCCAT GAAGCACCCC
    TCGACAGGGG TCCAGCTGCT CTCAGAACAG AAGGGCCTCT CACCATGCTG CTTCATCAGT
    GCGGAGGTGG TGCACTGGCT GGTGAATAAC GTGGAGGGTG TCCAGACCCA GGCGATGGCT
    ATCGACATCA TGCAGAAAAT GCTGGAAGAG CAACTCATCA CACACGCATC TGGTGAAGTC
    TGGCGGACCT TCATCTATGG CTTCTATTTC TACAAGATAG TCATGGACAA AGAACCTGAC
    CGAGTGGCCA TGCAGCAGCC CACCACCTGG CACACGACGG CGGGGGAGGA CTTCGCCAGC
    TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCCGAAG AGCTGTTGCA CTCCGAGATC
    CCTGCTTTCC TCTTGCCCTG GCTGCCCAGC CGACCAGCCT CCTATGCCAG CAGGCACAGC
    TCCTTCAGCC GCAGCTTTGG AGGGCGGAGC CAGGCAGCTG CACTTTTAGC TGCCACTGTC
    CCAGAGCAGA GGACTGTGAC CCTGGATGTG GACGTGAACA ACCGCACAGA CCGGCTGGAG
    TGGTGCAGCT GTTATTATCA TGGCAATTTC TCCCTGAATG CAGCTTTTGA GATCAAGCTC
    CACTGGATGG CTGTGACTGC TGCGGTCCTC TTCGAGATGG TCCAGGGTTG GCATCGCAAA
    GCCACTTCCT GCGGCTTTTT ATTGGTCCCT GTTCTGGAGG GTCCCTTTGC GCTGCCCAGT
    TACCTGTATG GCGATCCACT TCGAGCCCAG CTCTTCATCC CACTCAACAC CAGCTGCCTG
    CTCAAGGAGG GCAGTGAGCA CCTGTTTGAT AGCTTCGAGC CAGAAACATA CTGGGATCGA
    ATGCACCTTT TCCAGGAAGC CATTGCACAC AGGTTTGGAT TTGTGCAAGA TAAATATTCC
    GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCATGT TACAGGAACA
    GTGTTCCTGC AGCTGCCTTA CTCCAGGCGC AAGTTCTCCG GGCAGCAGCG GCGGCGGCGG
    AACTCCACCA GTTCCACCAA CCAGAACTTG TTCTGTGAGG AGCGTGTTGG CTACAACTGG
    GCCTACAACA CCATGCTGAC CAAGACATGG CGTTCTAGTG CCACGGGGGA TGAGAAGTTT
    GCTGATCGGC TGCTCAGGGA TTTCACGGAC TTCTGCATCA ACCGCGACAA CCGGCTGGTC
    ACATTCTGGA TGAGCTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26568
    Clone ID Related Accession (Same CDS sequence) XM_014108004.2 , XM_014108004.1
    Accession Version XM_014108004.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4548bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATGCC AGGCTTCTGT TGCACAGTTG GAGTGGACTG GAAGTCTCTC
    ACTACTCCGG CGTGCCTCCC CCTCACCACC GACTACTTCC CCGACCGCCA GGGCCTGCAG
    AACGACTACA CAGAGGGCTG TTACGATCTC CTTCCAGAAG CAGATATTGA CAGGAGGGAT
    GAGGAGGGTG TGCAGATGAC GGCCCAGCAG GTGTTTGAAG AGTTCATCTG CCAGCGTCTC
    ATGCAGGGCT ACCAAATCAT AGTGCAGCCC AAGGCACAAA AATCCAACCC CGTCGTCCCA
    CCCCCACTGA GCAGCAGCCC GCTCTATAGT CGAGGCCTTG TGTCCCGAAA CCGCCCTGAG
    GAGGAGGACC AATACTGGCT GAGTATGGGC AGAACGTTCC ACAAAGTGAC GCTGAAAGAT
    AAGATGATCA CAGTGACACG TTACCTTCCC AAGTACCCTT ATGAATCTGC CCAGATCCAC
    TACACCTACA GTCTCTGCCC TTCCCACTCA GACTCGGAGT TCGTCTCTTG TTGGGTGGAA
    TTCTCCCATG AGCGGCTGGA GGAGTACAAG TGGAATTACT TAGATCAGTA TATCTGTTCT
    GCTGGCTCTG AGGACTTCAG CTTAATTGAG TCTCTGAAGT TCTGGAGGAC CCGCTTCCTG
    CTGCTGCCAG CCTGTGTCAC TGCCACTAAG CGCATCACAG AAGGGGAGGC CCACTGCGAC
    ATCTATGGGG ACAGGCCTCG GGCAGACGAG GAGGAATGGC AGCTCTTGGA TGGTTTTATC
    CGCTTCGTGG AGGGTTTGAA CCGGATCCGC AGGCGACATC GCTCAGATCG CATGATGCGG
    AAAGGGACCG CCATGAAAGG TTTGCAGATG ACTGGGCCCA TTGCCACACA CTCCCTTGAG
    TCGACCGGCC CCCCAGTTGG GAAGAAGGGA ACCTCAGCTC TGTCCGCCCT GTTGGAGATG
    GAGGCCAGTC AGAAGTGCCT AGGAGAACAG CAGGCAGCTG TGCACGGTGG CAAGAGCGCC
    TCCCAGCCAG CTGAGAGCAG CAGCGTTGCC ATGACTCCCA CCTACGTGGA CAGCCCACGA
    AAGGACGGGG CCTTCTTTAT GGAGTTTGTC CGCAGCCCTC GCACAGCGTC ATCCGCCTTC
    TACCCACAGG TGTCTTTGGA CCAGACGGCC ACCCCGGTGT TAGACAGCAC CAGTTTAGGG
    GTAAGCACAG GCCAGTCTGT GGACAGAGGC AACAGCCAGG CCTTTGGGAA CTCCCAGAAC
    TCAGGGGAAC AGAGCTTCCC CTCTGCAAAC TCCAGTGATA GCAGCTCTCA GCAGCTGGCA
    GCAAGCTCTT TAACCTCATC CTCCACCTTG ACAGAGATCC TGGAAGCCAT GAAGCACCCC
    TCGACAGGGG TCCAGCTGCT CTCAGAACAG AAGGGCCTCT CACCATGCTG CTTCATCAGT
    GCGGAGGTGG TGCACTGGCT GGTGAATAAC GTGGAGGGTG TCCAGACCCA GGCGATGGCT
    ATCGACATCA TGCAGAAAAT GCTGGAAGAG CAACTCATCA CACACGCATC TGGTGAAGTC
    TGGCGGACCT TCATCTATGG CTTCTATTTC TACAAGATAG TCATGGACAA AGAACCTGAC
    CGAGTGGCCA TGCAGCAGCC CACCACCTGG CACACGACGG CGGGGGAGGA CTTCGCCAGC
    TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCCGAAG AGCTGTTGCA CTCCGAGATC
    CCTGCTTTCC TCTTGCCCTG GCTGCCCAGC CGACCAGCCT CCTATGCCAG CAGGCACAGC
    TCCTTCAGCC GCAGCTTTGG AGGGCGGAGC CAGGCAGCTG CACTTTTAGC TGCCACTGTC
    CCAGAGCAGA GGACTGTGAC CCTGGATGTG GACGTGAACA ACCGCACAGA CCGGCTGGAG
    TGGTGCAGCT GTTATTATCA TGGCAATTTC TCCCTGAATG CAGCTTTTGA GATCAAGCTC
    CACTGGATGG CTGTGACTGC TGCGGTCCTC TTCGAGATGG TCCAGGGTTG GCATCGCAAA
    GCCACTTCCT GCGGCTTTTT ATTGGTCCCT GTTCTGGAGG GTCCCTTTGC GCTGCCCAGT
    TACCTGTATG GCGATCCACT TCGAGCCCAG CTCTTCATCC CACTCAACAC CAGCTGCCTG
    CTCAAGGAGG GCAGTGAGCA CCTGTTTGAT AGCTTCGAGC CAGAAACATA CTGGGATCGA
    ATGCACCTTT TCCAGGAAGC CATTGCACAC AGGTTTGGAT TTGTGCAAGA TAAATATTCC
    GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCATGT TACAGGAACA
    GTGTTCCTGC AGCTGCCTTA CTCCAGGCGC AAGTTCTCCG GGCAGCAGCG GCGGCGGCGG
    AACTCCACCA GTTCCACCAA CCAGAACTTG TTCTGTGAGG AGCGTGTTGG CTACAACTGG
    GCCTACAACA CCATGCTGAC CAAGACATGG CGTTCTAGTG CCACGGGGGA TGAGAAGTTT
    GCTGATCGGC TGCTCAGGGA TTTCACGGAC TTCTGCATCA ACCGCGACAA CCGGCTGGTC
    ACATTCTGGA TGAGCTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963479.1
    CDS228..4775
    Translation

    Target ORF information:

    RefSeq Version XM_014108004.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108004.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATGCC AGGCTTCTGT TGCACAGTTG GAGTGGACTG GAAGTCTCTC
    ACTACTCCGG CGTGCCTCCC CCTCACCACC GACTACTTCC CCGACCGCCA GGGCCTGCAG
    AACGACTACA CAGAGGGCTG TTACGATCTC CTTCCAGAAG CAGATATTGA CAGGAGGGAT
    GAGGAGGGTG TGCAGATGAC GGCCCAGCAG GTGTTTGAAG AGTTCATCTG CCAGCGTCTC
    ATGCAGGGCT ACCAAATCAT AGTGCAGCCC AAGGCACAAA AATCCAACCC CGTCGTCCCA
    CCCCCACTGA GCAGCAGCCC GCTCTATAGT CGAGGCCTTG TGTCCCGAAA CCGCCCTGAG
    GAGGAGGACC AATACTGGCT GAGTATGGGC AGAACGTTCC ACAAAGTGAC GCTGAAAGAT
    AAGATGATCA CAGTGACACG TTACCTTCCC AAGTACCCTT ATGAATCTGC CCAGATCCAC
    TACACCTACA GTCTCTGCCC TTCCCACTCA GACTCGGAGT TCGTCTCTTG TTGGGTGGAA
    TTCTCCCATG AGCGGCTGGA GGAGTACAAG TGGAATTACT TAGATCAGTA TATCTGTTCT
    GCTGGCTCTG AGGACTTCAG CTTAATTGAG TCTCTGAAGT TCTGGAGGAC CCGCTTCCTG
    CTGCTGCCAG CCTGTGTCAC TGCCACTAAG CGCATCACAG AAGGGGAGGC CCACTGCGAC
    ATCTATGGGG ACAGGCCTCG GGCAGACGAG GAGGAATGGC AGCTCTTGGA TGGTTTTATC
    CGCTTCGTGG AGGGTTTGAA CCGGATCCGC AGGCGACATC GCTCAGATCG CATGATGCGG
    AAAGGGACCG CCATGAAAGG TTTGCAGATG ACTGGGCCCA TTGCCACACA CTCCCTTGAG
    TCGACCGGCC CCCCAGTTGG GAAGAAGGGA ACCTCAGCTC TGTCCGCCCT GTTGGAGATG
    GAGGCCAGTC AGAAGTGCCT AGGAGAACAG CAGGCAGCTG TGCACGGTGG CAAGAGCGCC
    TCCCAGCCAG CTGAGAGCAG CAGCGTTGCC ATGACTCCCA CCTACGTGGA CAGCCCACGA
    AAGGACGGGG CCTTCTTTAT GGAGTTTGTC CGCAGCCCTC GCACAGCGTC ATCCGCCTTC
    TACCCACAGG TGTCTTTGGA CCAGACGGCC ACCCCGGTGT TAGACAGCAC CAGTTTAGGG
    GTAAGCACAG GCCAGTCTGT GGACAGAGGC AACAGCCAGG CCTTTGGGAA CTCCCAGAAC
    TCAGGGGAAC AGAGCTTCCC CTCTGCAAAC TCCAGTGATA GCAGCTCTCA GCAGCTGGCA
    GCAAGCTCTT TAACCTCATC CTCCACCTTG ACAGAGATCC TGGAAGCCAT GAAGCACCCC
    TCGACAGGGG TCCAGCTGCT CTCAGAACAG AAGGGCCTCT CACCATGCTG CTTCATCAGT
    GCGGAGGTGG TGCACTGGCT GGTGAATAAC GTGGAGGGTG TCCAGACCCA GGCGATGGCT
    ATCGACATCA TGCAGAAAAT GCTGGAAGAG CAACTCATCA CACACGCATC TGGTGAAGTC
    TGGCGGACCT TCATCTATGG CTTCTATTTC TACAAGATAG TCATGGACAA AGAACCTGAC
    CGAGTGGCCA TGCAGCAGCC CACCACCTGG CACACGACGG CGGGGGAGGA CTTCGCCAGC
    TTCCAGCGCA AGTGGTTTGA GGTGGCCTTT GTGGCCGAAG AGCTGTTGCA CTCCGAGATC
    CCTGCTTTCC TCTTGCCCTG GCTGCCCAGC CGACCAGCCT CCTATGCCAG CAGGCACAGC
    TCCTTCAGCC GCAGCTTTGG AGGGCGGAGC CAGGCAGCTG CACTTTTAGC TGCCACTGTC
    CCAGAGCAGA GGACTGTGAC CCTGGATGTG GACGTGAACA ACCGCACAGA CCGGCTGGAG
    TGGTGCAGCT GTTATTATCA TGGCAATTTC TCCCTGAATG CAGCTTTTGA GATCAAGCTC
    CACTGGATGG CTGTGACTGC TGCGGTCCTC TTCGAGATGG TCCAGGGTTG GCATCGCAAA
    GCCACTTCCT GCGGCTTTTT ATTGGTCCCT GTTCTGGAGG GTCCCTTTGC GCTGCCCAGT
    TACCTGTATG GCGATCCACT TCGAGCCCAG CTCTTCATCC CACTCAACAC CAGCTGCCTG
    CTCAAGGAGG GCAGTGAGCA CCTGTTTGAT AGCTTCGAGC CAGAAACATA CTGGGATCGA
    ATGCACCTTT TCCAGGAAGC CATTGCACAC AGGTTTGGAT TTGTGCAAGA TAAATATTCC
    GCCTCTGCTT TTAACTTCCC TGCTGAGAAC AAGCCTCAGT ATATCCATGT TACAGGAACA
    GTGTTCCTGC AGCTGCCTTA CTCCAGGCGC AAGTTCTCCG GGCAGCAGCG GCGGCGGCGG
    AACTCCACCA GTTCCACCAA CCAGAACTTG TTCTGTGAGG AGCGTGTTGG CTACAACTGG
    GCCTACAACA CCATGCTGAC CAAGACATGG CGTTCTAGTG CCACGGGGGA TGAGAAGTTT
    GCTGATCGGC TGCTCAGGGA TTTCACGGAC TTCTGCATCA ACCGCGACAA CCGGCTGGTC
    ACATTCTGGA TGAGCTGCCT GGAGAAGATG CATGCCAGTG CCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26569
    Clone ID Related Accession (Same CDS sequence) XM_014108006.2 , XM_014108006.1
    Accession Version XM_014108006.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108006.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963481.1
    CDS216..4724
    Translation

    Target ORF information:

    RefSeq Version XM_014108006.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108006.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26569
    Clone ID Related Accession (Same CDS sequence) XM_014108006.2 , XM_014108006.1
    Accession Version XM_014108006.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963481.1
    CDS228..4736
    Translation

    Target ORF information:

    RefSeq Version XM_014108006.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108006.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GTATGAATCC TAGAACTCAG AATAAGGATT CTCTAGAGGA CAGTGTTTCT
    ACCTCTCCAG ACCCAATTCT GACACTGTCT GCTCCCCCTG TAGTGCCAGG CTTCTGTTGC
    ACAGTTGGAG TGGACTGGAA GTCTCTCACT ACTCCGGCGT GCCTCCCCCT CACCACCGAC
    TACTTCCCCG ACCGCCAGGG CCTGCAGAAC GACTACACAG AGGGCTGTTA CGATCTCCTT
    CCAGAAGCAG ATATTGACAG GAGGGATGAG GAGGGTGTGC AGATGACGGC CCAGCAGGTG
    TTTGAAGAGT TCATCTGCCA GCGTCTCATG CAGGGCTACC AAATCATAGT GCAGCCCAAG
    GCACAAAAAT CCAACCCCGT CGTCCCACCC CCACTGAGCA GCAGCCCGCT CTATAGTCGA
    GGCCTTGTGT CCCGAAACCG CCCTGAGGAG GAGGACCAAT ACTGGCTGAG TATGGGCAGA
    ACGTTCCACA AAGTGACGCT GAAAGATAAG ATGATCACAG TGACACGTTA CCTTCCCAAG
    TACCCTTATG AATCTGCCCA GATCCACTAC ACCTACAGTC TCTGCCCTTC CCACTCAGAC
    TCGGAGTTCG TCTCTTGTTG GGTGGAATTC TCCCATGAGC GGCTGGAGGA GTACAAGTGG
    AATTACTTAG ATCAGTATAT CTGTTCTGCT GGCTCTGAGG ACTTCAGCTT AATTGAGTCT
    CTGAAGTTCT GGAGGACCCG CTTCCTGCTG CTGCCAGCCT GTGTCACTGC CACTAAGCGC
    ATCACAGAAG GGGAGGCCCA CTGCGACATC TATGGGGACA GGCCTCGGGC AGACGAGGAG
    GAATGGCAGC TCTTGGATGG TTTTATCCGC TTCGTGGAGG GTTTGAACCG GATCCGCAGG
    CGACATCGCT CAGATCGCAT GATGCGGAAA GGGACCGCCA TGAAAGGTTT GCAGATGACT
    GGGCCCATTG CCACACACTC CCTTGAGTCG ACCGGCCCCC CAGTTGGGAA GAAGGGAACC
    TCAGCTCTGT CCGCCCTGTT GGAGATGGAG GCCAGTCAGA AGTGCCTAGG AGAACAGCAG
    GCAGCTGTGC ACGGTGGCAA GAGCGCCTCC CAGCCAGCTG AGAGCAGCAG CGTTGCCATG
    ACTCCCACCT ACGTGGACAG CCCACGAAAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGGA
    TTTGTGCAAG ATAAATATTC CGCCTCTGCT TTTAACTTCC CTGCTGAGAA CAAGCCTCAG
    TATATCCATG TTACAGGAAC AGTGTTCCTG CAGCTGCCTT ACTCCAGGCG CAAGTTCTCC
    GGGCAGCAGC GGCGGCGGCG GAACTCCACC AGTTCCACCA ACCAGAACTT GTTCTGTGAG
    GAGCGTGTTG GCTACAACTG GGCCTACAAC ACCATGCTGA CCAAGACATG GCGTTCTAGT
    GCCACGGGGG ATGAGAAGTT TGCTGATCGG CTGCTCAGGG ATTTCACGGA CTTCTGCATC
    AACCGCGACA ACCGGCTGGT CACATTCTGG ATGAGCTGCC TGGAGAAGAT GCATGCCAGT
    GCCCCGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26570
    Clone ID Related Accession (Same CDS sequence) XM_014108007.1 , XM_014108007.2
    Accession Version XM_014108007.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCAACTGC
    TTCATGCTGC TGTTTGTAAT ATGCTCAAGA GACACTTCCC CCACACCAGC TAGACTCTTC
    TGGGGTTTTC CACCTTCACT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963482.1
    CDS228..4691
    Translation

    Target ORF information:

    RefSeq Version XM_014108007.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108007.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCAACTGC
    TTCATGCTGC TGTTTGTAAT ATGCTCAAGA GACACTTCCC CCACACCAGC TAGACTCTTC
    TGGGGTTTTC CACCTTCACT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26570
    Clone ID Related Accession (Same CDS sequence) XM_014108007.1 , XM_014108007.2
    Accession Version XM_014108007.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4464bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCAACTGC
    TTCATGCTGC TGTTTGTAAT ATGCTCAAGA GACACTTCCC CCACACCAGC TAGACTCTTC
    TGGGGTTTTC CACCTTCACT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963482.1
    CDS217..4680
    Translation

    Target ORF information:

    RefSeq Version XM_014108007.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108007.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCAACTGC
    TTCATGCTGC TGTTTGTAAT ATGCTCAAGA GACACTTCCC CCACACCAGC TAGACTCTTC
    TGGGGTTTTC CACCTTCACT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26571
    Clone ID Related Accession (Same CDS sequence) XM_014108008.1 , XM_014108008.2
    Accession Version XM_014108008.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product DEP domain-containing protein 5 isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726106.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGTC
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963483.1
    CDS228..4673
    Translation

    Target ORF information:

    RefSeq Version XM_014108008.1
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108008.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGTC
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe26571
    Clone ID Related Accession (Same CDS sequence) XM_014108008.1 , XM_014108008.2
    Accession Version XM_014108008.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4446bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GATOR complex protein DEPDC5 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006608.3) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_014108008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGTC
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_013963483.1
    CDS216..4661
    Translation

    Target ORF information:

    RefSeq Version XM_014108008.2
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris DEP domain containing 5 (DEPDC5), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_014108008.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGAGAACAA CAAAAGTCTA CAAACTTGTC ATCCACAAGA AGGGCTTTGG GGGCAGTGAT 
    GATGAGCTAG TCATGAACCC TAAAGTGTTT CCTCACATCA AGCTTGGAGA CATTGTGGAG
    ATTGCTCACC CCAATGATGA ATACAGCCCT CTGCTTTTGC AGGTCAAGTC ACTTAAGGAA
    GATCTACAGA AAGAAACTAT TAGTGTGGAC CAGACTGTGA CTCAAGTGTT TCGACTAAGA
    CCTTATCAAG ATGTTTACGT GAATGTAGTA GACCCTAAGG ATGTGACCCT TGACCTAGTG
    GAATTAACCT TTAAGGATCA ATATATTGGC CGTGGGGATA TGTGGCGATT AAAGAAAAGT
    TTGGTCAGCA CATGTGCCTA TATCACTCAG AAGGTGGAAT TTGCTGGCAT CAGGGCACAG
    GCTGGTGAGC TGTGGGTCAA GAATGAGAAA GTCATGTGTG GTTATATCAG TGAAGATACC
    AGGGTGGTGT TCCGTTCTAC GTCGGCTATG GTTTACATAT TTATTCAGAT GAGCTGTGAA
    ATGTGGGATT TCGATATTTA TGGGGATCTA TATTTTGAGA AAGCTGTGAA TGGTTTCCTT
    GCTGATCTCT TTACCAAGTG GAAGGAGAAG AACTGTAGTC ATGAAGTGAC AGTGGTCCTA
    TTTTCTAGAA CTTTTTATGA TGCAAAATCT CTTGATGAAT TTCCTGAAAT AAACCGAGCC
    TCAATTCGAC AGGATCACAA GGGACGGTAC TATGAAGACT TTTATAAAGT GGTGGTGCAG
    AATGAGAGGA GAGAAGAGTG GACGTCACTC CTTGTGACCA TTAAAAAACT CTTCATCCAG
    TATCCAGTGT TGGTACGACT GGAACAGGCA GAGGGCTTTC CTCAAGGCGA CAATTCTACC
    TCAGCACAAG GAAACTACCT AGAGGCCATC AATTTGTCAT TCAATGTGTT TGACAAGCAC
    TACATCAACC GCAACTTTGA CCGAACTGGG CAGATGTCTG TGGTGATCAC GCCTGGGGTG
    GGTGTTTTTG AAGTGGACCG TCTACTTATG ATCTTGACCA AGCAGCGGAT GATAGATAAT
    GGAATTGGTG TGGACTTGGT GTGCATGGGG GAGCAACCAT TACATGCTGT CCCGCTGTTT
    AAGCTGCATA ATCGAAGTGC ACCTCGTGAT TCTCGTCTGG GCGATGACTA TAATATTCCT
    CACTGGATAA ACCACAGTTT CTATACGTCC AAAAGTCAGC TCTTTTGTAA CAGTTTCACC
    CCACGAATAA AACTGGCGGG AAAGAAGCCT GCCTCTGAGA AAGCAAAAAA TGGCCGTGAT
    ACATCTCTCG GGACTCCAAA AGAATCTGAG AACGCCCTTC CCATCCAAGT AGATTATGAT
    GCCTATGATG CCCAAGTGTT CAGGCTGCCA GGCCCATCCC GGGCTCAGCG CCTCGCCACC
    TGCAGGTCTG TGCGAGAACG GGACAGTCAC AATCGAAAGA GTGCCAGCTC CTGTGATGTC
    TCATCCAGCC CTTCCCTGCC CAGCCGTGCA CTGCCCACTG AAGAAGTGAG GAGCCAGGCT
    TCTGACGACA GCTCCCTGGG CAGGAGTGCC AATATCCTGA TGATCCCACA CCCCCACCTG
    CACCAGTATG AAGTCAGCAG TTCCTTGGGC TATACCAGCA CTCGAGATGT CCTGGAGAAC
    ATGATGGAGC CACCACAGCG AGACTCTAGC GCACCAGGGA GGTTCCATGT AGGCAGTGCG
    GAATCCATGC TGCATGTTCG ACCCGGGGGA TACACGCCCC AGCGGGCGCT CATTAACCCC
    TTTGCCCCCT CACGAATGCC CATGAAGCTC ACATCCAACA GAAGACGCTG GATGCACACT
    TTTCCTGTGG GACCATCCGG AGAGGCCATC CAGATCCATC ACCAGACCCG ACAGAATATG
    GCGGAGCTAC AGGGCAGCGG GCAGAGGGAC CCAGCTCACT CCTCTGCAGA GCTGCTGGAG
    TTAGCATATC AGGAAGCTGC TGGAAGGCAC AGCACTTCCC GCCAGCCTGG TGACAGCATG
    TCCTTCTTGA GCTTCAGTGG AACAGAAGAG CTTTCTGTCA GCCTGCTTAG CAACAGTGGT
    GCAGGTATGA ATCCTAGAAC TCAGAATAAG GATTCTCTAG AGGACAGTGT TTCTACCTCT
    CCAGACCCAA TTCTGACACT GTCTGCTCCC CCTGTAGTGC CAGGCTTCTG TTGCACAGTT
    GGAGTGGACT GGAAGTCTCT CACTACTCCG GCGTGCCTCC CCCTCACCAC CGACTACTTC
    CCCGACCGCC AGGGCCTGCA GAACGACTAC ACAGAGGGCT GTTACGATCT CCTTCCAGAA
    GCAGATATTG ACAGGAGGGA TGAGGAGGGT GTGCAGATGA CGGCCCAGCA GGTGTTTGAA
    GAGTTCATCT GCCAGCGTCT CATGCAGGGC TACCAAATCA TAGTGCAGCC CAAGGCACAA
    AAATCCAACC CCGTCGTCCC ACCCCCACTG AGCAGCAGCC CGCTCTATAG TCGAGGCCTT
    GTGTCCCGAA ACCGCCCTGA GGAGGAGGAC CAATACTGGC TGAGTATGGG CAGAACGTTC
    CACAAAGTGA CGCTGAAAGA TAAGATGATC ACAGTGACAC GTTACCTTCC CAAGTACCCT
    TATGAATCTG CCCAGATCCA CTACACCTAC AGTCTCTGCC CTTCCCACTC AGACTCGGAG
    TTCGTCTCTT GTTGGGTGGA ATTCTCCCAT GAGCGGCTGG AGGAGTACAA GTGGAATTAC
    TTAGATCAGT ATATCTGTTC TGCTGGCTCT GAGGACTTCA GCTTAATTGA GTCTCTGAAG
    TTCTGGAGGA CCCGCTTCCT GCTGCTGCCA GCCTGTGTCA CTGCCACTAA GCGCATCACA
    GAAGGGGAGG CCCACTGCGA CATCTATGGG GACAGGCCTC GGGCAGACGA GGAGGAATGG
    CAGCTCTTGG ATGGTTTTAT CCGCTTCGTG GAGGGTTTGA ACCGGATCCG CAGGCGACAT
    CGCTCAGATC GCATGATGCG GAAAGGGACC GCCATGAAAG GTTTGCAGAT GACTGGGCCC
    ATTGCCACAC ACTCCCTTGA GTCGACCGGC CCCCCAGTTG GGAAGAAGGG AACCTCAGCT
    CTGTCCGCCC TGTTGGAGAT GGAGGCCAGT CAGAAGTGCC TAGGAGAACA GCAGGCAGCT
    GTGCACGGTG GCAAGAGCGC CTCCCAGCCA GCTGAGAGCA GCAGCGTTGC CATGACTCCC
    ACCTACGTGG ACAGCCCACG AAAGGACGGG GCCTTCTTTA TGGAGTTTGT CCGCAGCCCT
    CGCACAGCGT CATCCGCCTT CTACCCACAG GTGTCTTTGG ACCAGACGGC CACCCCGGTG
    TTAGACAGCA CCAGTTTAGG GGTAAGCACA GGCCAGTCTG TGGACAGAGG CAACAGCCAG
    GCCTTTGGGA ACTCCCAGAA CTCAGGGGAA CAGAGCTTCC CCTCTGCAAA CTCCAGTGAT
    AGCAGCTCTC AGCAGCTGGC AGCAAGCTCT TTAACCTCAT CCTCCACCTT GACAGAGATC
    CTGGAAGCCA TGAAGCACCC CTCGACAGGG GTCCAGCTGC TCTCAGAACA GAAGGGCCTC
    TCACCATGCT GCTTCATCAG TGCGGAGGTG GTGCACTGGC TGGTGAATAA CGTGGAGGGT
    GTCCAGACCC AGGCGATGGC TATCGACATC ATGCAGAAAA TGCTGGAAGA GCAACTCATC
    ACACACGCAT CTGGTGAAGT CTGGCGGACC TTCATCTATG GCTTCTATTT CTACAAGATA
    GTCATGGACA AAGAACCTGA CCGAGTGGCC ATGCAGCAGC CCACCACCTG GCACACGACG
    GCGGGGGAGG ACTTCGCCAG CTTCCAGCGC AAGTGGTTTG AGGTGGCCTT TGTGGCCGAA
    GAGCTGTTGC ACTCCGAGAT CCCTGCTTTC CTCTTGCCCT GGCTGCCCAG CCGACCAGCC
    TCCTATGCCA GCAGGCACAG CTCCTTCAGC CGCAGCTTTG GAGGGCGGAG CCAGGCAGCT
    GCACTTTTAG CTGCCACTGT CCCAGAGCAG AGGACTGTGA CCCTGGATGT GGACGTGAAC
    AACCGCACAG ACCGGCTGGA GTGGTGCAGC TGTTATTATC ATGGCAATTT CTCCCTGAAT
    GCAGCTTTTG AGATCAAGCT CCACTGGATG GCTGTGACTG CTGCGGTCCT CTTCGAGATG
    GTCCAGGGTT GGCATCGCAA AGCCACTTCC TGCGGCTTTT TATTGGTCCC TGTTCTGGAG
    GGTCCCTTTG CGCTGCCCAG TTACCTGTAT GGCGATCCAC TTCGAGCCCA GCTCTTCATC
    CCACTCAACA CCAGCTGCCT GCTCAAGGAG GGCAGTGAGC ACCTGTTTGA TAGCTTCGAG
    CCAGAAACAT ACTGGGATCG AATGCACCTT TTCCAGGAAG CCATTGCACA CAGGTTTGTC
    CCCTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.