LOC105915744 cDNA ORF clone, Fundulus heteroclitus(mummichog)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC105915744
Entrez Gene ID	105915744
Full Name	uncharacterized LOC105915744
Gene Type	protein-coding
Organism	Fundulus heteroclitus(mummichog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_012849948.1	XP_012705402.1	uncharacterized protein LOC105915744
XM_021309241.1	XP_021164916.1	uncharacterized protein LOC105915744

Pathways from BioSystems
Gene Ontology

The following LOC105915744 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC105915744 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFu100062	XM_012849948.1 Latest version!	Fundulus heteroclitus uncharacterized LOC105915744 (LOC105915744), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
OFu127801	XM_021309241.1 Latest version!	Fundulus heteroclitus uncharacterized LOC105915744 (LOC105915744), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC105915744 ( XM_012849948.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_012705402.1 Fundulus heteroclitus uncharacterized LOC105915744 (LOC105915744), mRNA.

Price & Availability↑

CloneID	OFu100062
Clone ID Related Accession (Same CDS sequence)	XM_012849948.1
Accession Version	XM_012849948.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3153bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-06-14
Organism	Fundulus heteroclitus(Mummichog)
Product	uncharacterized protein LOC105915744
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224542.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 50% of CDS bases ##RefSeq-Attributes-END##

ATGGAGAGCT ACACGGAGTT TGTGCAGCTT CGTCTCTCTA AACTGCAGAA AAATGAGGAA 
GAAGAGGAGG AACGCCGCAG AAATTCACCA GCATCCTCCC TGATTCTCTT CTATGGACGA 
CCCATCCTTC CTCCTCTGCT CTCAGGGAAA CAAAGAGAAG AGATGAGGCG ACACAGAGAT 
GAAGCACAGA GAGCTGCAGC CAACCAGAAG CTCCAGGATG ACCCCAGAAT GGCCTACATC 
CAGACCATCC TGCACAGTGT TCAGCTGAGA AAGACGCCAA CGCTGCAGGA GTTACTCGAA 
GAAACGGAGA TTAAGGCCGA ATCTCCGCAC CCACACGACA CCAGCAGGTT CTCCACATCC 
TGCAGCGGCG CTCCTGTCAG AACCACGGAG AGCCTCTCCC CGGTGCCTGT CGGGAAATCA 
AAGAACGCCA TCTTTCTCCC TCCAAAGACC TCCACGACGT GCCGTGCCTT TTCCCCTTCA 
GCTGAATCGC CTCAGCAGAG CTACTGCGAA TCTTACCTCG CCGACCAGCT GGACAGCCGA 
CCAGGAGGCC AGCCATGCTC GTCTAACGCT GCGGGCCACC GGTCGCCCTC CTCCGGCTGT 
GTGACCCGGG AGAACCAGGA AAACTCCAGC TTTTACCTTC ACAACACCTC ACACACTATT 
TCCACGATGC CTGATATTAT CAGCCACCCT CCCATAGATG GAGAGGAACT GGAGAGGACC 
GGAGTGGAGT CGCCGTTTTG CATAAGCATA ATGGGAGCGG AAGGTGTCTG CAGTGCGTCG 
TTTCACAGCG ACTCAGTGAT GTGTGACCCT TTAAGAGCCG AACAGCCTGA ACGCAGTCAC 
CCACACAGTC TGAACAAAGA GGTCAACGTC CCCTTTACAG CAACACCGGA CCCTGCCAGC 
AGGAGCAGCG CGGAAAGAAA TGACGGATCG CTCGCTTTAT CCGACGGAGA CGGGCCGTCA 
GACAGTCCAG ATTTAGAGCG GTCGTTAAAT CTGACAAAAG AGGTCCACGG TCAGAAAATT 
CCCAGCAAGG CAGAAGTAGA GGCTGCAGAC AGTCAGATTG ATGTAGCGGA CTTAAAGCAC 
CCCGAGGATT CTCGTCCTCT AAGCCTGCAG GTCTTGCTGA AGAAGTCCCA GGAGTACCGG 
CGGCGCCAGC GGATGCTGAG GAACCAAGCC AAGAGCACTA AAACCCACCA GGAGCCGCCG 
AGGGCCCGGG CGGAGGAGCC GAGCCTCTCC GATAAGGAGA ACGATGAGTT GCATCACCAG 
GGCGCCGTGA CTGGAGAGGG CGGGAAGACC AAAGAAAGGA GAGGCTCTCT CATCCAGTCA 
GAGGAACCCG CGCCACAGAA ACCCTGGGAG GCCGACCCGA TTCAGGGCCG AGATAGAAAT 
GTAGCAGAAG ACGGGAGGAC AGCGGAGCGT CTCAGTGTTG AAGAGGAAAC CTCCTTAAAA 
AACAGGATTA ACAGCTCTCA AGAGGGCCTG ACTTCTCCTA AACAGATGGG CGTTCTGATC 
CAGCAGCAGC CGCTGTCAGC GAAGACCTCC CCCGTCCAAG GGGCGCTCTA TGACGCCCCC 
TGTCTTTCGT CCCTTCGCGG AGGAGTTGGG AAATACCGCT CGGTTCCGGT CCCAAGCCTC 
TGCCTGAGTC CCGTTCCCTG CAAAAGCAAA GCAGCCGCAG TCAGAGTTGA GGACCTGAGC 
CCGGATCGTG AACGGAGCTC CGCCGTGGAG CTCACGCGTG AAGACGACGC CGCAAGCGTG 
TTCGCCAAAA GCTCGCAGCA CATCGACCAG CTGGAGTCCA ACCTGTGCAG TCTGAAGGCG 
CTGATCTCAG ATCTGGAGTC CACGGTGAAG GGGAACCTGG ACGGTCTAGC TGACAGCAGC 
GTGCACAGCG AGGAGAGCAG CAAGGACTCT GAGGAAACCG AGACGGCTGG TCAGGATGGT 
TCCTCTGGCT CTTTGGAGGA CGCTCCGGCT AATGCTGCTG ATGGCAGTCA GGTAGAAGAC 
TTGCAGCAAA GACAATCGTT CATTCAGTTT AAGAACATGG ACGAAGACGT GGGACCTGAA 
CCGAGAACAC GTCGCAGAGA GGATGTTTCC CTCACAGCAC AGGACCGAGG GTCTGAGATC 
ATTAAACTAA GCAAACAGAG TCTGTTGACG GGCTCAAAAA GAGAGAATGG AAACCAGATG 
AGTGCTGAAG GAGGGAGCAG CAAAAAGGAG CAGCTGCCCT CTAAAGGTAT CCTGTCTTTC 
CGAAACGTTG CCTCCAAAAA CGTCCCTCAG TGCCACATTT CACTTCGGAG TCATCTCAAG 
GACAGAGAGG GAAATATAAC GCCCGAGGCT GAGGGCCCCG TCCCCTCGCC GTCCCTCAAC 
CTGTCATACG ACGTGGACAC GCCGTCGGAC CTTTGGTTCC AGGAAGGATC GGGATCCGAC 
GCCGGCTCAC GAGGCAGCCG TGCTCAGGGG AAACATCTGA CTCCAGGCAG CGGGGGCGAG 
GATCAGGTGG GCGTGTCTAA GGTCAAACGC AGGCTGCTCA TGCATGTGAC CGAGGGGAGC 
CTGGAGAGGG ACGAGGGCAC GGGGCGAGCC GCGGCCTCCG TGGTCAGGGC TGACCCAGGC 
ACTCCAAAAG CTGCGGTGCG TTGGTCTGAA GCCTGCAGCG TTCAGAGGCA AGAGCGGCTG 
AAGCAGGTCC ATGCAGCTCA GGTCAGAGCG CTGCAGGAGG CGCACAGGAA GCAGCAAGAA 
GAACTTCTAC AGGTGTTGGC AGCACGCTAC CGTCTCCTGC AGGACGCGTC TCCCTGCTCC 
ACAACAGGCT CTCGGTTCGG GGACACGTTG ACCTTCTCCA GCCTCTCCCA GCCTCCGAGC 
CCGCTCTCGC AGCGCTGCCG CCCCCTGCTG GCTGCAGCCG TGAAGGGTTT TCTAACTCGC 
AGGCTGCTGA GGACGGAGAG AGTCGCCCAG CTGGTGCGGA CCATCAGGGA CACGCAGCAG 
TTCCTGCAGG CCTTCCAGCA GAGGGGCACC GGCAGAGGAG AGCGCTGGAG CAGGCAGGAT 
GTATTATTGC AGGAGAGAGT TGCTCTGCAG CTGCGCGCCG CCCGTTACGA GGTTTACGAC 
ATATTCTTCA GTCTGTCAGC CAGAGAGCAG ACGCAGCTGA TCAGACGGGA CAGGGAGCTG 
GCCGGGGAGA GACGTCTGCG GCGGCAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_012705402.1
CDS	401..3553
Translation	MESYTEFVQLRLSKLQKNEEEEEERRRNSPASSLILFYGRPILPPLLSGKQREEMRRHRDEAQRAAANQKLQDDPRMAYIQTILHSVQLRKTPTLQELLEETEIKAESPHPHDTSRFSTSCSGAPVRTTESLSPVPVGKSKNAIFLPPKTSTTCRAFSPSAESPQQSYCESYLADQLDSRPGGQPCSSNAAGHRSPSSGCVTRENQENSSFYLHNTSHTISTMPDIISHPPIDGEELERTGVESPFCISIMGAEGVCSASFHSDSVMCDPLRAEQPERSHPHSLNKEVNVPFTATPDPASRSSAERNDGSLALSDGDGPSDSPDLERSLNLTKEVHGQKIPSKAEVEAADSQIDVADLKHPEDSRPLSLQVLLKKSQEYRRRQRMLRNQAKSTKTHQEPPRARAEEPSLSDKENDELHHQGAVTGEGGKTKERRGSLIQSEEPAPQKPWEADPIQGRDRNVAEDGRTAERLSVEEETSLKNRINSSQEGLTSPKQMGVLIQQQPLSAKTSPVQGALYDAPCLSSLRGGVGKYRSVPVPSLCLSPVPCKSKAAAVRVEDLSPDRERSSAVELTREDDAASVFAKSSQHIDQLESNLCSLKALISDLESTVKGNLDGLADSSVHSEESSKDSEETETAGQDGSSGSLEDAPANAADGSQVEDLQQRQSFIQFKNMDEDVGPEPRTRRREDVSLTAQDRGSEIIKLSKQSLLTGSKRENGNQMSAEGGSSKKEQLPSKGILSFRNVASKNVPQCHISLRSHLKDREGNITPEAEGPVPSPSLNLSYDVDTPSDLWFQEGSGSDAGSRGSRAQGKHLTPGSGGEDQVGVSKVKRRLLMHVTEGSLERDEGTGRAAASVVRADPGTPKAAVRWSEACSVQRQERLKQVHAAQVRALQEAHRKQQEELLQVLAARYRLLQDASPCSTTGSRFGDTLTFSSLSQPPSPLSQRCRPLLAAAVKGFLTRRLLRTERVAQLVRTIRDTQQFLQAFQQRGTGRGERWSRQDVLLQERVALQLRAARYEVYDIFFSLSAREQTQLIRRDRELAGERRLRRQL

Target ORF information:

RefSeq Version	XM_012849948.1
Organism	Fundulus heteroclitus(Mummichog)
Definition	Fundulus heteroclitus uncharacterized LOC105915744 (LOC105915744), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_012849948.1

ORF Insert Sequence:

ATGGAGAGCT ACACGGAGTT TGTGCAGCTT CGTCTCTCTA AACTGCAGAA AAATGAGGAA 
GAAGAGGAGG AACGCCGCAG AAATTCACCA GCATCCTCCC TGATTCTCTT CTATGGACGA 
CCCATCCTTC CTCCTCTGCT CTCAGGGAAA CAAAGAGAAG AGATGAGGCG ACACAGAGAT 
GAAGCACAGA GAGCTGCAGC CAACCAGAAG CTCCAGGATG ACCCCAGAAT GGCCTACATC 
CAGACCATCC TGCACAGTGT TCAGCTGAGA AAGACGCCAA CGCTGCAGGA GTTACTCGAA 
GAAACGGAGA TTAAGGCCGA ATCTCCGCAC CCACACGACA CCAGCAGGTT CTCCACATCC 
TGCAGCGGCG CTCCTGTCAG AACCACGGAG AGCCTCTCCC CGGTGCCTGT CGGGAAATCA 
AAGAACGCCA TCTTTCTCCC TCCAAAGACC TCCACGACGT GCCGTGCCTT TTCCCCTTCA 
GCTGAATCGC CTCAGCAGAG CTACTGCGAA TCTTACCTCG CCGACCAGCT GGACAGCCGA 
CCAGGAGGCC AGCCATGCTC GTCTAACGCT GCGGGCCACC GGTCGCCCTC CTCCGGCTGT 
GTGACCCGGG AGAACCAGGA AAACTCCAGC TTTTACCTTC ACAACACCTC ACACACTATT 
TCCACGATGC CTGATATTAT CAGCCACCCT CCCATAGATG GAGAGGAACT GGAGAGGACC 
GGAGTGGAGT CGCCGTTTTG CATAAGCATA ATGGGAGCGG AAGGTGTCTG CAGTGCGTCG 
TTTCACAGCG ACTCAGTGAT GTGTGACCCT TTAAGAGCCG AACAGCCTGA ACGCAGTCAC 
CCACACAGTC TGAACAAAGA GGTCAACGTC CCCTTTACAG CAACACCGGA CCCTGCCAGC 
AGGAGCAGCG CGGAAAGAAA TGACGGATCG CTCGCTTTAT CCGACGGAGA CGGGCCGTCA 
GACAGTCCAG ATTTAGAGCG GTCGTTAAAT CTGACAAAAG AGGTCCACGG TCAGAAAATT 
CCCAGCAAGG CAGAAGTAGA GGCTGCAGAC AGTCAGATTG ATGTAGCGGA CTTAAAGCAC 
CCCGAGGATT CTCGTCCTCT AAGCCTGCAG GTCTTGCTGA AGAAGTCCCA GGAGTACCGG 
CGGCGCCAGC GGATGCTGAG GAACCAAGCC AAGAGCACTA AAACCCACCA GGAGCCGCCG 
AGGGCCCGGG CGGAGGAGCC GAGCCTCTCC GATAAGGAGA ACGATGAGTT GCATCACCAG 
GGCGCCGTGA CTGGAGAGGG CGGGAAGACC AAAGAAAGGA GAGGCTCTCT CATCCAGTCA 
GAGGAACCCG CGCCACAGAA ACCCTGGGAG GCCGACCCGA TTCAGGGCCG AGATAGAAAT 
GTAGCAGAAG ACGGGAGGAC AGCGGAGCGT CTCAGTGTTG AAGAGGAAAC CTCCTTAAAA 
AACAGGATTA ACAGCTCTCA AGAGGGCCTG ACTTCTCCTA AACAGATGGG CGTTCTGATC 
CAGCAGCAGC CGCTGTCAGC GAAGACCTCC CCCGTCCAAG GGGCGCTCTA TGACGCCCCC 
TGTCTTTCGT CCCTTCGCGG AGGAGTTGGG AAATACCGCT CGGTTCCGGT CCCAAGCCTC 
TGCCTGAGTC CCGTTCCCTG CAAAAGCAAA GCAGCCGCAG TCAGAGTTGA GGACCTGAGC 
CCGGATCGTG AACGGAGCTC CGCCGTGGAG CTCACGCGTG AAGACGACGC CGCAAGCGTG 
TTCGCCAAAA GCTCGCAGCA CATCGACCAG CTGGAGTCCA ACCTGTGCAG TCTGAAGGCG 
CTGATCTCAG ATCTGGAGTC CACGGTGAAG GGGAACCTGG ACGGTCTAGC TGACAGCAGC 
GTGCACAGCG AGGAGAGCAG CAAGGACTCT GAGGAAACCG AGACGGCTGG TCAGGATGGT 
TCCTCTGGCT CTTTGGAGGA CGCTCCGGCT AATGCTGCTG ATGGCAGTCA GGTAGAAGAC 
TTGCAGCAAA GACAATCGTT CATTCAGTTT AAGAACATGG ACGAAGACGT GGGACCTGAA 
CCGAGAACAC GTCGCAGAGA GGATGTTTCC CTCACAGCAC AGGACCGAGG GTCTGAGATC 
ATTAAACTAA GCAAACAGAG TCTGTTGACG GGCTCAAAAA GAGAGAATGG AAACCAGATG 
AGTGCTGAAG GAGGGAGCAG CAAAAAGGAG CAGCTGCCCT CTAAAGGTAT CCTGTCTTTC 
CGAAACGTTG CCTCCAAAAA CGTCCCTCAG TGCCACATTT CACTTCGGAG TCATCTCAAG 
GACAGAGAGG GAAATATAAC GCCCGAGGCT GAGGGCCCCG TCCCCTCGCC GTCCCTCAAC 
CTGTCATACG ACGTGGACAC GCCGTCGGAC CTTTGGTTCC AGGAAGGATC GGGATCCGAC 
GCCGGCTCAC GAGGCAGCCG TGCTCAGGGG AAACATCTGA CTCCAGGCAG CGGGGGCGAG 
GATCAGGTGG GCGTGTCTAA GGTCAAACGC AGGCTGCTCA TGCATGTGAC CGAGGGGAGC 
CTGGAGAGGG ACGAGGGCAC GGGGCGAGCC GCGGCCTCCG TGGTCAGGGC TGACCCAGGC 
ACTCCAAAAG CTGCGGTGCG TTGGTCTGAA GCCTGCAGCG TTCAGAGGCA AGAGCGGCTG 
AAGCAGGTCC ATGCAGCTCA GGTCAGAGCG CTGCAGGAGG CGCACAGGAA GCAGCAAGAA 
GAACTTCTAC AGGTGTTGGC AGCACGCTAC CGTCTCCTGC AGGACGCGTC TCCCTGCTCC 
ACAACAGGCT CTCGGTTCGG GGACACGTTG ACCTTCTCCA GCCTCTCCCA GCCTCCGAGC 
CCGCTCTCGC AGCGCTGCCG CCCCCTGCTG GCTGCAGCCG TGAAGGGTTT TCTAACTCGC 
AGGCTGCTGA GGACGGAGAG AGTCGCCCAG CTGGTGCGGA CCATCAGGGA CACGCAGCAG 
TTCCTGCAGG CCTTCCAGCA GAGGGGCACC GGCAGAGGAG AGCGCTGGAG CAGGCAGGAT 
GTATTATTGC AGGAGAGAGT TGCTCTGCAG CTGCGCGCCG CCCGTTACGA GGTTTACGAC 
ATATTCTTCA GTCTGTCAGC CAGAGAGCAG ACGCAGCTGA TCAGACGGGA CAGGGAGCTG 
GCCGGGGAGA GACGTCTGCG GCGGCAGCTT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC105915744 ( XM_021309241.1 ) cDNA ORF clone, Fundulus heteroclitus(mummichog) -> XP_021164916.1 Fundulus heteroclitus uncharacterized LOC105915744 (LOC105915744), mRNA.

Price & Availability↑

CloneID	OFu127801
Clone ID Related Accession (Same CDS sequence)	XM_021309241.1
Accession Version	XM_021309241.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3390bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-05-31
Organism	Fundulus heteroclitus(mummichog)
Product	uncharacterized protein LOC105915744
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_012224542.1) and transcript sequence (GCES01029427.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Fundulus heteroclitus Annotation Release 101 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 83 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAGAGCT ACACGGAGTT TGTGCAGCTT CGTCTCTCTA AACTGCAGAA AAATGAGGAA 
GAAGAGGAGG AACGCCGCAG AAATTCACCA GCATCCTCCC TGATTCTCTT CTATGGACGA 
CCCATCCTTC CTCCTCTGCT CTCAGGGAAA CAAAGAGAAG AGATGAGGCG ACACAGAGAT 
GAAGCACAGA GAGCTGCAGC CAACCAGAAG CTCCAGGATG ACCCCAGAAT GGCCTACATC 
CAGACCATCC TGCACAGTGT TCAGCTGAGA AAGACGCCAA CGCTGCAGGA GTTACTCGAA 
GAAACGGAGA TTAAGGCCGA ATCTCCGCAC CCACACGACA CCAGCAGGTT CTCCACATCC 
TGCAGCGGCG CTCCTGTCAG AACCACGGAG AGCCTCTCCC CGGTGCCTGT CGGGAAATCA 
AAGAACGCCA TCTTTCTCCC TCCAAAGACC TCCACGACGT GCCGTGCCTT TTCCCCTTCA 
GCTGAATCGC CTCAGCAGAG CTACTGCGAA TCTTACCTCG CCGACCAGCT GGACAGCCGA 
CCAGGAGGCC AGCCATGCTC GTCTAACGCT GCGGGCCACC GGTCGCCCTC CTCCGGCTGT 
GTGACCCGGG AGAACCAGGA AAACTCCAGC TTTTACCTTC ACAACACCTC ACACACTATT 
TCCACGATGC CTGATATTAT CAGCCACCCT CCCATAGATG GAGAGGAACT GGAGAGGACC 
GGAGTGGAGT CGCCGTTTTG CATAAGCATA ATGGGAGCGG AAGGTGTCTG CAGTGCGTCG 
TTTCACAGCG ACTCAGTGAT GTGTGACCCT TTAAGAGCCG AACAGCCTGA ACGCAGTCAC 
CCACACAGTC TGAACAAAGA GGTCAACGTC CCCTTTACAG CAACACCGGA CCCTGCCAGC 
AGGAGCAGCG CGGAAAGAAA TGACGGATCG CTCGCTTTAT CCGACGGAGA CGGGCCGTCA 
GACAGTCCAG ATTTAGAGCG GTCGTTAAAT CTGACAAAAG AGGTCCACGG TCAGAAAATT 
CCCAGCAAGG CAGAAGTAGA GGCTGCAGAC AGTCAGATTG ATGTAGCGGA CTTAAAGCAC 
CCCGAGGATT CTCGTCCTCT AAGCCTGCAG GTCTTGCTGA AGAAGTCCCA GGAGTACCGG 
CGGCGCCAGC GGATGCTGAG GAACCAAGCC AAGAGCACTA AAACCCACCA GGAGCCGCCG 
AGGGCCCGGG CGGAGGAGCC GAGCCTCTCC GATAAGGAGA ACGATGAGTT GCATCACCAG 
GGCGCCGTGA CTGGAGAGGG CGGGAAGACC AAAGAAAGGA GAGGCTCTCT CATCCAGTCA 
GAGGAACCCG CGCCACAGAA ACCCTGGGAG GCCGACCCGA TTCAGGGCCG AGATAGAAAT 
GTAGCAGAAG ACGGGAGGAC AGCGGAGCGT CTCAGTGTTG AAGAGGAAAC CTCCTTAAAA 
AACAGGATTA ACAGCTCTCA AGAGGGCCTG ACTTCTCCTA AACAGATGGG CGTTCTGATC 
CAGCAGCAGC CGCTGTCAGC GAAGACCTCC CCCGTCCAAG GGGCGCTCTA TGACGCCCCC 
TGTCTTTCGT CCCTTCGCGG AGGAGTTGGG AAATACCGCT CGGTTCCGGT CCCAAGCCTC 
TGCCTGAGTC CCGTTCCCTG CAAAAGCAAA GCAGCCGCAG TCAGAGTTGA GGACCTGAGC 
CCGGATCGTG AACGGAGCTC CGCCGTGGAG CTCACGCGTG AAGACGACGC CGCAAGCGTG 
TTCGCCAAAA GCTCGCAGCA CATCGACCAG CTGGAGTCCA ACCTGTGCAG TCTGAAGGCG 
CTGATCTCAG ATCTGGAGTC CACGGTGAAG GGGAACCTGG ACGGTCTAGC TGACAGCAGC 
GTGCACAGCG AGGAGAGCAG CAAGGACTCT GAGGAAACCG AGACGGCTGG TCAGGATGGT 
TCCTCTGGCT CTTTGGAGGA CGCTCCGGCT AATGCTGCTG ATGGCAGTCA GGTAGAAGAC 
TTGCAGCAAA GACAATCGTT CATTCAGTTT AAGAACATGG ACGAAGACGT GGGACCTGAA 
CCGAGAACAC GTCGCAGAGA GGATGTTTCC CTCACAGCAC AGGACCGAGG GTCTGAGATC 
ATTAAACTAA GCAAACAGAG TCTGTTGACG GGCTCAAAAA GAGAGAATGG AAACCAGATG 
AGTGCTGAAG GAGGGAGCAG CAAAAAGGAG CAGCTGCCCT CTAAAGGTAT CCTGTCTTTC 
CGAAACGTTG CCTCCAAAAA CGTCCCTCAG TGCCACATTT CACTTCGGAG TCATCTCAAG 
GACAGAGAGG GAAATATAAC GCCCGAGGCT GAGGGCCCCG TCCCCTCGCC GTCCCTCAAC 
CTGTCATACG ACGTGGACAC GCCGTCGGAC CTTTGGTTCC AGGAAGGATC GGGATCCGAC 
GCCGGCTCAC GAGGCAGCCG TGCTCAGGGG AAACATCTGA CTCCAGGCAG CGGGGGCGAG 
GATCAGGTGG GCGTGTCTAA GGTCAAACGC AGGCTGCTCA TGCATGTGAC CGAGGGGAGC 
CTGGAGAGGG ACGAGGGCAC GGGGCGAGCC GCGGCCTCCG TGGTCAGGGC TGACCCAGGC 
ACTCCAAAAG CTGCGGTGCG TTGGTCTGAA GCCTGCAGCG TTCAGAGGCA AGAGCGGCTG 
AAGCAGGTCC ATGCAGCTCA GGTCAGAGCG CTGCAGGAGG CGCACAGGAA GCAGCAAGAA 
GAACTTCTAC AGGTGTTGGC AGCACGCTAC CGTCTCCTGC AGGACGCGTC TCCCTGCTCC 
ACAACAGGCT CTCGGTTCGG GGACACGTTG ACCTTCTCCA GCCTCTCCCA GCCTCCGAGC 
CCGCTCTCGC AGCGCTGCCG CCCCCTGCTG GCTGCAGCCG TGAAGGGTTT TCTAACTCGC 
AGGCTGCTGA GGACGGAGAG AGTCGCCCAG CTGGTGCGGA CCATCAGGGA CACGCAGCAG 
TTCCTGCAGG CCTTCCAGCA GAGGGGCACC GGCAGAGGAG AGCGCTGGAG CAGGCAGGAT 
GTATTATTGC AGGAGAGAGT TGCTCTGCAG CTGCGCGCCG CCCGTTACGA GGTTTACGAC 
ATATTCTTCA GTCTGTCAGC CAGAGAGCAG ACGCAGCTGA TCAGACGGGA CAGGGAGCTG 
GCCGGGGAGA GACGTCTGCG GCGGCAGAAC GGACCTGCAG GTGACCACCG AGGAAAGAGT 
TCTCTGTCAG CTGCGACGCA GAAATCCCTG GAGAGGAAGC GAGAAAGGAT GATTCAGAAA 
AGGACCGCAG AATGTCACGG GGGAGCTGTG GTGCGGACCG GCCAAAAGTC TAGATTCTCA 
GCCGAGAAAC CACCGGAGAC AAAACGAGGA CAGTTTAAAG CGAATCCTCA GAGAGTTCCC 
AAAAGCGTTT CCTCGTCTAG ACCTCGATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021164916.1
CDS	399..3788
Translation	MESYTEFVQLRLSKLQKNEEEEEERRRNSPASSLILFYGRPILPPLLSGKQREEMRRHRDEAQRAAANQKLQDDPRMAYIQTILHSVQLRKTPTLQELLEETEIKAESPHPHDTSRFSTSCSGAPVRTTESLSPVPVGKSKNAIFLPPKTSTTCRAFSPSAESPQQSYCESYLADQLDSRPGGQPCSSNAAGHRSPSSGCVTRENQENSSFYLHNTSHTISTMPDIISHPPIDGEELERTGVESPFCISIMGAEGVCSASFHSDSVMCDPLRAEQPERSHPHSLNKEVNVPFTATPDPASRSSAERNDGSLALSDGDGPSDSPDLERSLNLTKEVHGQKIPSKAEVEAADSQIDVADLKHPEDSRPLSLQVLLKKSQEYRRRQRMLRNQAKSTKTHQEPPRARAEEPSLSDKENDELHHQGAVTGEGGKTKERRGSLIQSEEPAPQKPWEADPIQGRDRNVAEDGRTAERLSVEEETSLKNRINSSQEGLTSPKQMGVLIQQQPLSAKTSPVQGALYDAPCLSSLRGGVGKYRSVPVPSLCLSPVPCKSKAAAVRVEDLSPDRERSSAVELTREDDAASVFAKSSQHIDQLESNLCSLKALISDLESTVKGNLDGLADSSVHSEESSKDSEETETAGQDGSSGSLEDAPANAADGSQVEDLQQRQSFIQFKNMDEDVGPEPRTRRREDVSLTAQDRGSEIIKLSKQSLLTGSKRENGNQMSAEGGSSKKEQLPSKGILSFRNVASKNVPQCHISLRSHLKDREGNITPEAEGPVPSPSLNLSYDVDTPSDLWFQEGSGSDAGSRGSRAQGKHLTPGSGGEDQVGVSKVKRRLLMHVTEGSLERDEGTGRAAASVVRADPGTPKAAVRWSEACSVQRQERLKQVHAAQVRALQEAHRKQQEELLQVLAARYRLLQDASPCSTTGSRFGDTLTFSSLSQPPSPLSQRCRPLLAAAVKGFLTRRLLRTERVAQLVRTIRDTQQFLQAFQQRGTGRGERWSRQDVLLQERVALQLRAARYEVYDIFFSLSAREQTQLIRRDRELAGERRLRRQNGPAGDHRGKSSLSAATQKSLERKRERMIQKRTAECHGGAVVRTGQKSRFSAEKPPETKRGQFKANPQRVPKSVSSSRPR

Target ORF information:

RefSeq Version	XM_021309241.1
Organism	Fundulus heteroclitus(mummichog)
Definition	Fundulus heteroclitus uncharacterized LOC105915744 (LOC105915744), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021309241.1

ORF Insert Sequence:

ATGGAGAGCT ACACGGAGTT TGTGCAGCTT CGTCTCTCTA AACTGCAGAA AAATGAGGAA 
GAAGAGGAGG AACGCCGCAG AAATTCACCA GCATCCTCCC TGATTCTCTT CTATGGACGA 
CCCATCCTTC CTCCTCTGCT CTCAGGGAAA CAAAGAGAAG AGATGAGGCG ACACAGAGAT 
GAAGCACAGA GAGCTGCAGC CAACCAGAAG CTCCAGGATG ACCCCAGAAT GGCCTACATC 
CAGACCATCC TGCACAGTGT TCAGCTGAGA AAGACGCCAA CGCTGCAGGA GTTACTCGAA 
GAAACGGAGA TTAAGGCCGA ATCTCCGCAC CCACACGACA CCAGCAGGTT CTCCACATCC 
TGCAGCGGCG CTCCTGTCAG AACCACGGAG AGCCTCTCCC CGGTGCCTGT CGGGAAATCA 
AAGAACGCCA TCTTTCTCCC TCCAAAGACC TCCACGACGT GCCGTGCCTT TTCCCCTTCA 
GCTGAATCGC CTCAGCAGAG CTACTGCGAA TCTTACCTCG CCGACCAGCT GGACAGCCGA 
CCAGGAGGCC AGCCATGCTC GTCTAACGCT GCGGGCCACC GGTCGCCCTC CTCCGGCTGT 
GTGACCCGGG AGAACCAGGA AAACTCCAGC TTTTACCTTC ACAACACCTC ACACACTATT 
TCCACGATGC CTGATATTAT CAGCCACCCT CCCATAGATG GAGAGGAACT GGAGAGGACC 
GGAGTGGAGT CGCCGTTTTG CATAAGCATA ATGGGAGCGG AAGGTGTCTG CAGTGCGTCG 
TTTCACAGCG ACTCAGTGAT GTGTGACCCT TTAAGAGCCG AACAGCCTGA ACGCAGTCAC 
CCACACAGTC TGAACAAAGA GGTCAACGTC CCCTTTACAG CAACACCGGA CCCTGCCAGC 
AGGAGCAGCG CGGAAAGAAA TGACGGATCG CTCGCTTTAT CCGACGGAGA CGGGCCGTCA 
GACAGTCCAG ATTTAGAGCG GTCGTTAAAT CTGACAAAAG AGGTCCACGG TCAGAAAATT 
CCCAGCAAGG CAGAAGTAGA GGCTGCAGAC AGTCAGATTG ATGTAGCGGA CTTAAAGCAC 
CCCGAGGATT CTCGTCCTCT AAGCCTGCAG GTCTTGCTGA AGAAGTCCCA GGAGTACCGG 
CGGCGCCAGC GGATGCTGAG GAACCAAGCC AAGAGCACTA AAACCCACCA GGAGCCGCCG 
AGGGCCCGGG CGGAGGAGCC GAGCCTCTCC GATAAGGAGA ACGATGAGTT GCATCACCAG 
GGCGCCGTGA CTGGAGAGGG CGGGAAGACC AAAGAAAGGA GAGGCTCTCT CATCCAGTCA 
GAGGAACCCG CGCCACAGAA ACCCTGGGAG GCCGACCCGA TTCAGGGCCG AGATAGAAAT 
GTAGCAGAAG ACGGGAGGAC AGCGGAGCGT CTCAGTGTTG AAGAGGAAAC CTCCTTAAAA 
AACAGGATTA ACAGCTCTCA AGAGGGCCTG ACTTCTCCTA AACAGATGGG CGTTCTGATC 
CAGCAGCAGC CGCTGTCAGC GAAGACCTCC CCCGTCCAAG GGGCGCTCTA TGACGCCCCC 
TGTCTTTCGT CCCTTCGCGG AGGAGTTGGG AAATACCGCT CGGTTCCGGT CCCAAGCCTC 
TGCCTGAGTC CCGTTCCCTG CAAAAGCAAA GCAGCCGCAG TCAGAGTTGA GGACCTGAGC 
CCGGATCGTG AACGGAGCTC CGCCGTGGAG CTCACGCGTG AAGACGACGC CGCAAGCGTG 
TTCGCCAAAA GCTCGCAGCA CATCGACCAG CTGGAGTCCA ACCTGTGCAG TCTGAAGGCG 
CTGATCTCAG ATCTGGAGTC CACGGTGAAG GGGAACCTGG ACGGTCTAGC TGACAGCAGC 
GTGCACAGCG AGGAGAGCAG CAAGGACTCT GAGGAAACCG AGACGGCTGG TCAGGATGGT 
TCCTCTGGCT CTTTGGAGGA CGCTCCGGCT AATGCTGCTG ATGGCAGTCA GGTAGAAGAC 
TTGCAGCAAA GACAATCGTT CATTCAGTTT AAGAACATGG ACGAAGACGT GGGACCTGAA 
CCGAGAACAC GTCGCAGAGA GGATGTTTCC CTCACAGCAC AGGACCGAGG GTCTGAGATC 
ATTAAACTAA GCAAACAGAG TCTGTTGACG GGCTCAAAAA GAGAGAATGG AAACCAGATG 
AGTGCTGAAG GAGGGAGCAG CAAAAAGGAG CAGCTGCCCT CTAAAGGTAT CCTGTCTTTC 
CGAAACGTTG CCTCCAAAAA CGTCCCTCAG TGCCACATTT CACTTCGGAG TCATCTCAAG 
GACAGAGAGG GAAATATAAC GCCCGAGGCT GAGGGCCCCG TCCCCTCGCC GTCCCTCAAC 
CTGTCATACG ACGTGGACAC GCCGTCGGAC CTTTGGTTCC AGGAAGGATC GGGATCCGAC 
GCCGGCTCAC GAGGCAGCCG TGCTCAGGGG AAACATCTGA CTCCAGGCAG CGGGGGCGAG 
GATCAGGTGG GCGTGTCTAA GGTCAAACGC AGGCTGCTCA TGCATGTGAC CGAGGGGAGC 
CTGGAGAGGG ACGAGGGCAC GGGGCGAGCC GCGGCCTCCG TGGTCAGGGC TGACCCAGGC 
ACTCCAAAAG CTGCGGTGCG TTGGTCTGAA GCCTGCAGCG TTCAGAGGCA AGAGCGGCTG 
AAGCAGGTCC ATGCAGCTCA GGTCAGAGCG CTGCAGGAGG CGCACAGGAA GCAGCAAGAA 
GAACTTCTAC AGGTGTTGGC AGCACGCTAC CGTCTCCTGC AGGACGCGTC TCCCTGCTCC 
ACAACAGGCT CTCGGTTCGG GGACACGTTG ACCTTCTCCA GCCTCTCCCA GCCTCCGAGC 
CCGCTCTCGC AGCGCTGCCG CCCCCTGCTG GCTGCAGCCG TGAAGGGTTT TCTAACTCGC 
AGGCTGCTGA GGACGGAGAG AGTCGCCCAG CTGGTGCGGA CCATCAGGGA CACGCAGCAG 
TTCCTGCAGG CCTTCCAGCA GAGGGGCACC GGCAGAGGAG AGCGCTGGAG CAGGCAGGAT 
GTATTATTGC AGGAGAGAGT TGCTCTGCAG CTGCGCGCCG CCCGTTACGA GGTTTACGAC 
ATATTCTTCA GTCTGTCAGC CAGAGAGCAG ACGCAGCTGA TCAGACGGGA CAGGGAGCTG 
GCCGGGGAGA GACGTCTGCG GCGGCAGAAC GGACCTGCAG GTGACCACCG AGGAAAGAGT 
TCTCTGTCAG CTGCGACGCA GAAATCCCTG GAGAGGAAGC GAGAAAGGAT GATTCAGAAA 
AGGACCGCAG AATGTCACGG GGGAGCTGTG GTGCGGACCG GCCAAAAGTC TAGATTCTCA 
GCCGAGAAAC CACCGGAGAC AAAACGAGGA CAGTTTAAAG CGAATCCTCA GAGAGTTCCC 
AAAAGCGTTT CCTCGTCTAG ACCTCGATGA