trpm2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	trpm2
Entrez Gene ID	799412
Full Name	transient receptor potential cation channel, subfamily M, member 2
Synonyms	si:ch73-263b18.1
General protein information	Preferred Names transient receptor potential cation channel, subfamily M, member 2 Names transient receptor potential cation channel subfamily M member 2 transient receptor potential melastatin 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009304992.2	XP_009303267.1	transient receptor potential cation channel subfamily M member 2 isoform X1
XM_021478778.1	XP_021334453.1	transient receptor potential cation channel subfamily M member 2 isoform X1
NM_001288817.1	NP_001275746.1	transient receptor potential cation channel subfamily M member 2
XM_017357728.1	XP_017213217.1	transient receptor potential cation channel subfamily M member 2 isoform X2
XM_017357729.1	XP_017213218.1	transient receptor potential cation channel subfamily M member 2 isoform X3
XM_009304992.3	XP_009303267.1	transient receptor potential cation channel subfamily M member 2 isoform X2
XM_009304991.2	XP_009303266.1	transient receptor potential cation channel subfamily M member 2 isoform X1
XM_021478779.1	XP_021334454.1	transient receptor potential cation channel subfamily M member 2 isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC565414	XP_693785.6
Danio rerio (zebrafish)	LOC558375	XP_686669.1
Danio rerio (zebrafish)	ftr29	XP_003198034.1
Danio rerio (zebrafish)	ftr82	NP_001068571.1
Danio rerio (zebrafish)	LOC560422	XP_688915.1
Danio rerio (zebrafish)	LOC100000832	XP_001340954.2
Danio rerio (zebrafish)	LOC100006636	XP_005172385.1
Danio rerio (zebrafish)	ftr73	XP_005157866.1
Danio rerio (zebrafish)	ftr11	XP_003197971.1
Danio rerio (zebrafish)	LOC101882560	XP_005163612.1
Danio rerio (zebrafish)	ftr67	NP_001034808.2
Danio rerio (zebrafish)	ftr34	XP_005163669.1
Danio rerio (zebrafish)	ftr37	XP_696445.3
Danio rerio (zebrafish)	ftr30	XP_003198033.2
Danio rerio (zebrafish)	btr22	XP_005173784.1
Danio rerio (zebrafish)	btr18	XP_005173787.1
Danio rerio (zebrafish)	btr32	XP_003201305.1
Danio rerio (zebrafish)	btr07	XP_001923237.4
Danio rerio (zebrafish)	LOC100001589	XP_001341559.3
Danio rerio (zebrafish)	ftr79	XP_697299.2
Danio rerio (zebrafish)	ftr83	XP_688583.3
Danio rerio (zebrafish)	LOC100333853	XP_002661845.3
Danio rerio (zebrafish)	si:dkeyp-123a12.2	XP_691204.4
Danio rerio (zebrafish)	LOC100007791	XP_001346158.5
Danio rerio (zebrafish)	si:ch211-250k18.8	XP_698776.5
Danio rerio (zebrafish)	zgc:174944	NP_001108057.1
Danio rerio (zebrafish)	LOC566957	XP_005174040.1
Danio rerio (zebrafish)	LOC100150225	XP_001921257.4
Danio rerio (zebrafish)	LOC100334101	XP_003197833.2
Danio rerio (zebrafish)	LOC100334843	XP_002665168.2
Danio rerio (zebrafish)	si:ch73-24k9.2	XP_002665507.3
Danio rerio (zebrafish)	si:ch211-196h16.5	XP_690098.5
Danio rerio (zebrafish)	si:ch211-194e1.7	XP_005167387.1
Danio rerio (zebrafish)	LOC793291	XP_001331884.5
Danio rerio (zebrafish)	LOC100004711	XP_001340175.3
Danio rerio (zebrafish)	LOC100534746	XP_005161332.1
Danio rerio (zebrafish)	LOC100005625	XP_001339269.4
Danio rerio (zebrafish)	LOC100003765	XP_001343238.2
Danio rerio (zebrafish)	LOC101883156	XP_005160435.1
Danio rerio (zebrafish)	si:ch211-237a4.2	XP_003198575.2
Danio rerio (zebrafish)	zgc:194906	NP_001124145.1
Danio rerio (zebrafish)	LOC100536980	XP_003201699.2
Danio rerio (zebrafish)	si:ch211-15j1.3	XP_002660683.2
Danio rerio (zebrafish)	si:ch211-233g6.6	XP_002660707.3
Danio rerio (zebrafish)	LOC100331097	XP_002661460.3
Danio rerio (zebrafish)	LOC101885873	XP_005161560.1
Danio rerio (zebrafish)	LOC101883187	XP_005174457.1
Danio rerio (zebrafish)	LOC101882512	XP_005171750.1
Danio rerio (zebrafish)	LOC100329306	XP_005168148.1
Danio rerio (zebrafish)	LOC100535915	XP_005161831.1
Danio rerio (zebrafish)	si:ch211-114l13.1	XP_002660672.3
Danio rerio (zebrafish)	LOC101885187	XP_005165034.1
Danio rerio (zebrafish)	si:rp71-7l19.2	XP_003198343.2
Danio rerio (zebrafish)	LOC100331680	XP_002667178.3
Danio rerio (zebrafish)	LOC100005516	XP_005171758.1
Danio rerio (zebrafish)	LOC101883084	XP_005174455.1
Danio rerio (zebrafish)	LOC101883426	XP_005174069.1
Danio rerio (zebrafish)	LOC100150280	XP_005174567.1
Danio rerio (zebrafish)	LOC101884637	XP_005168127.1
Danio rerio (zebrafish)	LOC100329543	XP_005167374.1
Danio rerio (zebrafish)	LOC101882306	XP_005171748.1
Danio rerio (zebrafish)	LOC566508	XP_694871.6
Danio rerio (zebrafish)	LOC101884187	XP_005157862.1
Danio rerio (zebrafish)	ftr55	XP_003200609.2
Danio rerio (zebrafish)	LOC100330813	XP_002660428.2
Danio rerio (zebrafish)	ftr49	XP_003198077.1
Danio rerio (zebrafish)	LOC100535633	XP_003198959.1
Danio rerio (zebrafish)	btr04	XP_003198956.2
Danio rerio (zebrafish)	btr09	NP_001124100.1
Danio rerio (zebrafish)	LOC558675	XP_687012.2
Danio rerio (zebrafish)	ftr76	XP_005157864.1
Danio rerio (zebrafish)	ftr16	XP_690796.5
Danio rerio (zebrafish)	LOC561829	XP_690320.2
Danio rerio (zebrafish)	ftr03	XP_002660930.2
Danio rerio (zebrafish)	ftr06	XP_001337856.1
Danio rerio (zebrafish)	LOC570185	XP_698724.1
Danio rerio (zebrafish)	LOC797531	XP_001338000.2
Danio rerio (zebrafish)	ftr02	XP_003197969.1
Danio rerio (zebrafish)	LOC571442	XP_700123.5
Danio rerio (zebrafish)	ftr19	XP_002660960.3
Danio rerio (zebrafish)	LOC101882488	XP_005163611.1
Danio rerio (zebrafish)	ftr39p	XP_002661035.2
Danio rerio (zebrafish)	zgc:136302	XP_005170221.1
Danio rerio (zebrafish)	ftr58	XP_686163.5
Danio rerio (zebrafish)	LOC100537283	XP_003198007.2
Danio rerio (zebrafish)	ftr64	XP_002661885.1
Danio rerio (zebrafish)	btr31	XP_005170663.1
Danio rerio (zebrafish)	LOC100006950	XP_001345543.5
Danio rerio (zebrafish)	ftr28l	XP_005163705.1
Danio rerio (zebrafish)	LOC100333325	XP_002667878.3
Danio rerio (zebrafish)	si:ch211-121a2.3	XP_698105.5
Danio rerio (zebrafish)	LOC799802	XP_001336792.2
Danio rerio (zebrafish)	LOC555438	XP_683030.6
Danio rerio (zebrafish)	LOC568716	XP_003201051.2
Danio rerio (zebrafish)	LOC568664	XP_003201050.2
Danio rerio (zebrafish)	si:ch73-286h23.3	XP_001346239.5
Danio rerio (zebrafish)	LOC100006645	XP_001337665.3
Danio rerio (zebrafish)	wu:fb15h11	XP_697949.6
Danio rerio (zebrafish)	si:ch211-191a16.2	XP_001338550.5
Danio rerio (zebrafish)	si:ch211-181d7.1	XP_001919795.3
Danio rerio (zebrafish)	LOC795182	XP_001332957.4
Danio rerio (zebrafish)	LOC100329836	XP_002664746.3
Danio rerio (zebrafish)	LOC100334547	XP_002664742.2
Danio rerio (zebrafish)	si:dkey-156m2.3	XP_002664795.3
Danio rerio (zebrafish)	si:ch211-213a13.6	XP_697815.6
Danio rerio (zebrafish)	si:dkey-13n15.11	XP_005165261.1
Danio rerio (zebrafish)	si:zfos-364h11.1	XP_002665163.2
Danio rerio (zebrafish)	LOC100002266	XP_005158672.1
Danio rerio (zebrafish)	si:ch211-278p9.3	XP_002665167.3
Danio rerio (zebrafish)	zgc:136791	XP_005160238.1
Danio rerio (zebrafish)	LOC796214	XP_005161889.1
Danio rerio (zebrafish)	LOC100536939	XP_003200134.2
Danio rerio (zebrafish)	zgc:174178	NP_001107787.1
Danio rerio (zebrafish)	LOC101882503	XP_005168090.1
Danio rerio (zebrafish)	si:ch211-114l13.4	XP_002660681.1
Danio rerio (zebrafish)	si:ch211-15j1.5	XP_002660682.2
Danio rerio (zebrafish)	LOC100334715	XP_005171097.1
Danio rerio (zebrafish)	si:ch211-15j1.1	XP_001920778.3
Danio rerio (zebrafish)	si:dkey-222h21.8	XP_002660686.2
Danio rerio (zebrafish)	si:dkey-222h21.5	XP_002660689.1
Danio rerio (zebrafish)	LOC101882744	XP_005168242.1
Danio rerio (zebrafish)	LOC100329650	XP_002661801.3
Danio rerio (zebrafish)	si:ch211-255g12.8	XP_694303.5
Danio rerio (zebrafish)	LOC100331576	XP_002661628.3
Danio rerio (zebrafish)	LOC101883760	XP_005164630.1
Danio rerio (zebrafish)	LOC101886147	XP_005167368.1
Danio rerio (zebrafish)	LOC101886579	XP_005161565.1
Danio rerio (zebrafish)	LOC100333957	XP_005171081.1
Danio rerio (zebrafish)	LOC101884123	XP_005171093.1
Danio rerio (zebrafish)	LOC101884874	XP_005171101.1
Danio rerio (zebrafish)	LOC100333264	XP_002660697.3
Danio rerio (zebrafish)	LOC100330744	XP_002660427.3
Danio rerio (zebrafish)	ftr50	XP_005164403.1
Danio rerio (zebrafish)	LOC793898	XP_001920428.2
Danio rerio (zebrafish)	LOC791496	XP_003198035.2
Danio rerio (zebrafish)	LOC565787	XP_694149.5
Danio rerio (zebrafish)	LOC570079	XP_698604.5
Danio rerio (zebrafish)	LOC570163	XP_003197979.1
Danio rerio (zebrafish)	btr16	XP_005157512.1
Danio rerio (zebrafish)	LOC570251	XP_698802.4
Danio rerio (zebrafish)	ftr15	XP_700104.3
Danio rerio (zebrafish)	ftr26	XP_005163617.1
Danio rerio (zebrafish)	ftr65	XP_003198031.2
Danio rerio (zebrafish)	zgc:173666	XP_003198037.1
Danio rerio (zebrafish)	ftr61	XP_003201315.1
Danio rerio (zebrafish)	btr20	XP_003200561.1
Danio rerio (zebrafish)	zgc:173581	XP_005173811.1
Danio rerio (zebrafish)	btr06	NP_001120951.1
Danio rerio (zebrafish)	LOC101886307	XP_005163673.1
Danio rerio (zebrafish)	LOC101884427	XP_005159614.1
Danio rerio (zebrafish)	LOC100002745	XP_005164415.1
Danio rerio (zebrafish)	LOC101885734	XP_005161559.1
Danio rerio (zebrafish)	si:dkey-151g22.1	NP_001137518.1
Danio rerio (zebrafish)	si:dkey-121n8.4	XP_001335920.4
Danio rerio (zebrafish)	LOC100330073	XP_002661806.3
Danio rerio (zebrafish)	si:dkey-7i4.1	XP_005169721.1
Danio rerio (zebrafish)	LOC100001465	XP_005166707.1
Danio rerio (zebrafish)	LOC100005483	XP_001339130.3
Danio rerio (zebrafish)	LOC562679	XP_691146.5
Danio rerio (zebrafish)	LOC100333840	XP_005169858.1
Danio rerio (zebrafish)	LOC100536924	XP_003200567.2
Danio rerio (zebrafish)	LOC559196	XP_687605.5
Danio rerio (zebrafish)	LOC795109	XP_001335224.5
Danio rerio (zebrafish)	si:ch211-187g4.1	XP_698878.6
Danio rerio (zebrafish)	LOC100538217	XP_005169783.1
Danio rerio (zebrafish)	LOC571651	XP_700351.5
Danio rerio (zebrafish)	si:ch211-271g18.1	XP_002661463.3
Danio rerio (zebrafish)	LOC565381	XP_693750.6
Danio rerio (zebrafish)	si:ch211-238e22.7	XP_005168160.1
Danio rerio (zebrafish)	LOC100333747	XP_002661530.3
Danio rerio (zebrafish)	LOC100331072	XP_002661905.3
Danio rerio (zebrafish)	si:ch73-236c18.7	XP_002660699.3
Danio rerio (zebrafish)	si:dkey-222h21.6	XP_002660688.2
Danio rerio (zebrafish)	si:ch211-233g6.3	XP_002660709.3
Danio rerio (zebrafish)	LOC100329354	XP_002661501.3
Danio rerio (zebrafish)	LOC100006280	XP_001920132.4
Danio rerio (zebrafish)	LOC101886178	XP_005164994.1
Danio rerio (zebrafish)	LOC101883660	XP_005168155.1
Danio rerio (zebrafish)	LOC101883625	XP_005170693.1
Danio rerio (zebrafish)	LOC100150791	XP_005165031.1
Danio rerio (zebrafish)	LOC101883234	XP_005160282.1
Danio rerio (zebrafish)	LOC101885938	XP_005173831.1
Danio rerio (zebrafish)	LOC101885252	XP_005168541.1
Danio rerio (zebrafish)	trpm2	XP_003198602.2
Danio rerio (zebrafish)	btr12	NP_001070087.1
Danio rerio (zebrafish)	btr02	NP_001070626.2
Danio rerio (zebrafish)	ftr72	NP_001007388.2
Danio rerio (zebrafish)	LOC101883527	XP_005170222.1
Danio rerio (zebrafish)	trim25l	XP_005172141.1
Danio rerio (zebrafish)	LOC100149991	XP_003200554.1
Danio rerio (zebrafish)	LOC101883221	XP_005171452.1
Danio rerio (zebrafish)	ftr56	XP_003200605.1
Danio rerio (zebrafish)	LOC100334710	XP_002665516.2
Danio rerio (zebrafish)	btr25	XP_005173808.1
Danio rerio (zebrafish)	ftr24	XP_003197988.1
Danio rerio (zebrafish)	LOC100536343	XP_003197990.1
Danio rerio (zebrafish)	LOC100537128	XP_003200077.2
Danio rerio (zebrafish)	LOC100536410	XP_003200565.2
Danio rerio (zebrafish)	btr21	XP_003200564.2
Danio rerio (zebrafish)	ftr84	XP_005165427.1
Danio rerio (zebrafish)	si:ch211-150h8.3	XP_003198032.1
Danio rerio (zebrafish)	ftr43	XP_005164401.1
Danio rerio (zebrafish)	LOC571488	XP_700178.4
Danio rerio (zebrafish)	ftr41	XP_695494.5
Danio rerio (zebrafish)	ftr59	XP_686796.2
Danio rerio (zebrafish)	ftr51	XP_691169.5
Danio rerio (zebrafish)	ftr14l	XP_003199766.1
Danio rerio (zebrafish)	si:ch211-214b16.3	XP_005164324.1
Danio rerio (zebrafish)	LOC100331532	XP_003201602.2
Danio rerio (zebrafish)	LOC100329480	XP_002663890.3
Danio rerio (zebrafish)	si:dkey-222h21.2	XP_001334961.4
Danio rerio (zebrafish)	LOC100006564	NP_001119957.1
Danio rerio (zebrafish)	LOC100333746	XP_002661485.3
Danio rerio (zebrafish)	LOC100329448	XP_002667651.3
Danio rerio (zebrafish)	si:ch211-114l13.10	XP_696367.5
Danio rerio (zebrafish)	LOC100331159	XP_005169797.1
Danio rerio (zebrafish)	si:ch211-213a13.9	XP_003201049.2
Danio rerio (zebrafish)	si:dkey-253d23.10	XP_683789.6
Danio rerio (zebrafish)	si:ch211-278p9.4	XP_001336197.5
Danio rerio (zebrafish)	LOC566782	XP_695160.6
Danio rerio (zebrafish)	LOC558818	NP_001038341.1
Danio rerio (zebrafish)	si:ch211-149a19.3	XP_005170041.1
Danio rerio (zebrafish)	si:dkey-222h21.9	XP_002660685.1
Danio rerio (zebrafish)	LOC796962	XP_001923433.2
Danio rerio (zebrafish)	LOC100536895	XP_003200133.2
Danio rerio (zebrafish)	LOC100537076	XP_003200135.2
Danio rerio (zebrafish)	LOC100329927	XP_003200136.2
Danio rerio (zebrafish)	si:ch211-233m11.1	XP_687146.4
Danio rerio (zebrafish)	LOC100535360	XP_003200157.2
Danio rerio (zebrafish)	LOC101884992	XP_005174036.1
Danio rerio (zebrafish)	LOC794214	XP_001334125.1
Danio rerio (zebrafish)	LOC100537399	XP_003198502.2
Danio rerio (zebrafish)	LOC100007745	XP_001346125.5
Danio rerio (zebrafish)	si:dkey-126g1.7	XP_005162400.1
Danio rerio (zebrafish)	LOC101882643	XP_005171752.1
Danio rerio (zebrafish)	LOC100001765	XP_001341704.4
Danio rerio (zebrafish)	si:dkey-82i20.1	XP_002666374.3
Danio rerio (zebrafish)	LOC100333748	XP_002661966.3
Danio rerio (zebrafish)	LOC565296	XP_003198495.2
Danio rerio (zebrafish)	LOC100535428	XP_005174493.1
Danio rerio (zebrafish)	LOC100334677	XP_002661542.3
Danio rerio (zebrafish)	LOC100329558	XP_002661549.3
Danio rerio (zebrafish)	si:dkeyp-4c4.1	NP_001094426.1
Danio rerio (zebrafish)	LOC101885760	XP_005164976.1
Danio rerio (zebrafish)	LOC101884884	XP_005157350.1
Danio rerio (zebrafish)	LOC101885212	XP_005158663.1
Danio rerio (zebrafish)	LOC101885461	XP_005168195.1
Danio rerio (zebrafish)	LOC560232	NP_001116726.1
Danio rerio (zebrafish)	LOC101885169	XP_005174039.1
Danio rerio (zebrafish)	LOC101883611	XP_005174070.1

Gene Ontology
Bibliography

Related articles in PubMed

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Jer?nimo R, Moraes MN, de Assis LVM, Ramos BC, Rocha T, Castrucci AML
Journal of thermal biology68(Pt A)128-138(2017 Aug)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following trpm2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the trpm2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa144000	XM_021478779.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa143999	XM_021478778.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa35834	NM_001288817.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa47363	XM_009304991.2 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa47363	XM_009304992.3 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa47364	XM_017357728.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa47365	XM_017357729.1 Latest version!	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
View Old Accession Versions >>
ODa47363	XM_009304992.2	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

trpm2 ( XM_021478779.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334454.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa144000
Clone ID Related Accession (Same CDS sequence)	XM_021478779.1
Accession Version	XM_021478779.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4197bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA AAGCATGCAC ATGTCCTTTG TATGTTCATA TTACAACATG TATGCACACG 
TTAAATCTAC ATCTAATGCA AAAAGATAAA AATAAATGCA TGTTTTTGTT TGTAGAAATG 
CTGGGTACTT CAGGGGTGAA AATCCACCCA AATGGCAACA GCAACCAGTT AGGAGTCCAG 
CTAGAAAATG TGAAACTGAC AAGTTTATTC AAAAAACTTG ATAAGCGCTG CTCACTCGCC 
TCCTGGATTA AAGAAAACAT CAAGAAGAAA GAGTGCTGCT TCTATGTGGA GGATGGCAGG 
GAGGGGATTT GTAAGTGTGG TTACCCGAAG GTCCAGCACT GCGATGAGGC CATCAAACCA 
GAGGACTATA TGGGGGAACA GTGGGACAAA CACAGGCACG TCAGAGAGAC ACCCACCGAT 
GCCTTTGGAG ATATTAGTTT TGGAGGACTT GGACAGAAAA CCGGGAAGTA TGTCCGGGTG 
TCTTCTGACA CGTCTTGTGA GAATCTGTAC CAGTTGATGA CAGAACAGTG GAAACTGCGT 
TCACCAAACC TGCTGATTTC CGTGACCGGC GGAGCCAAAA ACTTCTACAT CAAGACGCAC 
CTGAAGGACA AGTTCCGCCG CGGCCTCATA AAAGTCGCCC AGACCACAGG TGCGTGGATC 
CTGACGGGAG GAACTCATGC GGGTGTGATG AAGCATGTGG GCATGGCTGT GAGGGATTAT 
ACACTCAGCA GTGGGTCAAT GGAGGGACAG ATCGTCGTCA TCGGTGTTGC GCCCTGGGGA 
GTCATTCACA ACCGCAGCAC ACTCATCCAT CCTGAGGGCC GTTTTCCGGC GTATTACTCT 
CTGGATGAGC AGGGTCAGGG CCGTCTCTCC TGCCTCGACA TCAATCACAC TCACTTCCTG 
CTGGTGGATG ATGGCACTCA AGGACATTAT GGGGTTGAGA TTGAGCTGCG GGCCCGTCTG 
GAGAAACTTA TCTCTAAACT ATCACTCGGA AATAGAGAGA GTGGAGTGAC GATACCAGTG 
GTCTGTGTGG TTCTGGACGG CGGTCCGGGA ACTCTAAATA CGATCTATAA TTCGATGCTG 
AACCACACGC CGTGTGTTGT ACTGGAGGGC TCTGGGCGTC TGGCGGATGT TATCGCTCAT 
GTGGCCTCTG TGCCCGTGTC AAAGGTCACA ATGGCTCTCA TCAACCGTCT CCTGAAGAGG 
TTTTTCATGC AAGAGTACAA AAACTTCACC GAGTTGCAGA TCATTGAGTG GACTAAGAAG 
ATTCAGGACA TCTTGCGGAT GCCTCATTTG CTCACAGTAT TCCGCATCGA TGAGGATAAA 
AACTATGACG TGGATGTGGC CATTCTGCAG GCGCTGCTAA AAGCCTCCAG AAGTGATGAA 
CATGCAGGTC GGCATTGCTG GGAGAGGCAG CTGGAGCTCG CTGTGGCCTG GAACCGCGTG 
GACATCGCAG AGAGCGAGAT CTTCACCGAG GAGAGCCAGT GGACGTCCAG TGATCTGCAT 
CCAGCCATGT TCTCCGCTCT GGTTGGTGAT AAGCCCGAGT TTGTGCGCCT CCTGCTGGAG 
AACGGTGTGT GTGTGCGTGA GTTTCTGGAG CGTGAGGAGA CTCTCTGCGA GCTCTACTCT 
CATCTGCCCT CCTGCTTCTT CCTTCGCAAA TTGGCCAAAC GGGTCCAGGG CGGAAAGATG 
CGGCGCGGGC AGGAGCCACT GCCCGGCAGC AGGAAGGTCT GCCTGAGTCA CGTCTCTGAA 
GAAGTGCGAC ACCTGCTGGG CAGCTTCACA CAACCGCTGT ACATTGCTTC TCGATACAAG 
CCCACTAAAG ATGATGTGCG CTTAAAGGTG CCGTCTAAAG GTGCTCTGGA TCTTCCATGT 
TCGGGTGAGG AATGGAGCGC AGACACAGTT TGGGATCCTG GACGGGATCT GTTCCTCTGG 
GCTGTAGTGC AGAATAACAG AGAGCTGGCG GAGATCGGTT GGGAGCAGTG CAGAGACTGT 
ATCGCTGCTG CTCTGGCCGC CAGTAAGATC TTGAGGAAGC TAGCGCAGGA GAGCGGAGAG 
GATGACAGTG AAGAGGCTAC AGAGATGCTG GAGCTCGCCA ACCACTACGA GAAACAGGCC 
ATCGGTGTGT TCAGCGAGTG CCACAGCTGG GACGCGCAGC GGGCGCAGAA GCTGCTCATC 
CGCATCTCTC CATCATGGGG CAGAAGCACA TGCCTGTGGC TCGCTCTGGA GGCTCACGAT 
AAAAGCTTCA TCGCTCACTC CGGAGTTCAG GCTCTTCTGA CTCAGATCTG GTGTGGAGAG 
CTGTCTGTAG ATAATCCACA CTGGAAAGTG CTGCTCTGCA TGATCTTCTT TCCTCTCATC 
TACACCGGCT TCCTGACCTT CAGACGGGAT GAAGACATTC AGAGACAGGC CGAACGGACG 
GAGCAGCAGA AACTGGCCAT GGAGTCTGTG TTTGCTGGAC AGTCAGACGG CAAAATCAAA 
CGTCATTTAC GTGGATTTTC ACAGAAGTCT GAGCTGAAGC CTTTGAACTG TTCGTCTCGT 
CTGATGAGTT TCCTCAAGTC TCCTCAAGTC AAGTTTTACT GGAACATCGC CTCATATTTC 
GGCTTCCTGT GGCTGTTTGC TGTGGTGCTC ATGATCGATT TCCAGACCTC GCCATCATGG 
AGGGAACTGC TGCTGTATGT GTGGCTCACG TCGCTGGTGT GTGAGGAGAT TCGACAGCTC 
TATCATGACT TTGACGGCTC TGGATTTCGG AGGAAAGCAA AGATGTACAT CAAGGATCTG 
TGGAACATTC TGGATGTGCT GTCAATCGTG CTGTTCATTG CTGGTCTAAT CTGCAGACTT 
CAGGCCTCGG ACACTGTGTT TTACATTGGT AAAGTCATCC TCTGCATCGA CTTCATCATA 
TTCTGCCTGC GATTAATGGC CATCTTCTCC ATCAGTCGAA CTCTGGGTCC CAAAATAATC 
ATCGTCAGGA GAATGATGTT GGATTTGTTT TTCTTCATGT TTCTGCTCAG TATCTGGGTT 
GTGGCGTACG GAGTAGCCAA ACAGGGCATT CTGATCGAAA ACGAAGAGAG ACTAAACTGG 
ATCATCCGTG GAGCTGTTTA TGAGCCCTAC ATCACTATAT TTGGCAACTT CCCTACTAAT 
ATAGACAATA CTCTGTTTGA TATTAGCAGC TGTAGTGTGA ACGCCTCGGA TCCTCTGAAG 
CCCAAATGTC CCATGCTGAA CGCCGATAAC ACTCCGGTGT TCCCAGAATG GCTGACCATC 
ATGATGCTGT GTGTTTATCT GCTGTTCGCC AACATCCTTT TACTCAACCT GCTCATCGCC 
ATCTTTAACT ACACGTTTCA GGAGGTTCAG GACAACACAG ACACCATCTG GAAGTTTCAG 
CGGTACGAGC TGATAAAGGA GTATCACAGC CGACCTGCTC TTCCTCCACC CTTCATCCTC 
CTAAGTCACC TCATCCTCTT CATCAGAGGA GTTTTCCTGC GTGACCTGCC GCAGAGACAC 
AAGAACTTCC GACAGGAGCT GGAGCAGACG GAGGAAGAGG AGCTGTTATC ATGGGAAGCC 
TACATGAAGG ACAACTATCT GGCATCCACA AGACAGGACG AGAGCCAGAG CGTGGAGCAT 
CGCATCCATG ACACGGCAGA GAAGGTTGGA GCGATGTCTG AACTGCTGGA GCGCGAGCAG 
GAGATGGTTT CTGCCACTAT GGCCAAACGA CTGGCCAGGC TTGAAGAGCA GGTCTCGGAG 
TCTGCCAAAG CTTTGCGATG GATTATTGAT GCGCTGAAAT CACAAGGCTG CAAATCCAAA 
GTGCAGCCTC CATTAATGAG AAGTAAAAGC TCTGATAGGG ATGATGGAGA CTCCAGCGGA 
CAGGAGACGG ATGATGAAGA GGCTCCTCAT ATGTTTGCTC GTCAGCTGCA ATATCCTGAC 
AGCACCGTCA GACGCTTTCC TGTGCCTGAG GAGAAGGTGT CCTGGGAGGT AAACTTCAGC 
CCTTATCAAC CTCCAGTTTA CAATCAACAG GACAGCAGCG AATCTGACAC TTCAGCCTTA 
GACAAGCACA GGAATCCCGG TGGCAGGACT GGGATACGAG GAAAAGGGGC TCTGAATACC 
CTTGGTCCGA ATCACATTCT CCACCCCATT TTCACCAGGT GTGGCTCTGA AATCCTGAAA 
TCTGCTCTTC TTTCAGCATC ACATGCAGGA ATTCTAATCC TATTTCAAGT GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334454.1
CDS	71..4267
Translation	MAKKACTCPLYVHITTCMHTLNLHLMQKDKNKCMFLFVEMLGTSGVKIHPNGNSNQLGVQLENVKLTSLFKKLDKRCSLASWIKENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDILRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLLIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILLSHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRCGSEILKSALLSASHAGILILFQVA

Target ORF information:

RefSeq Version	XM_021478779.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478779.1

ORF Insert Sequence:

ATGGCAAAAA AAGCATGCAC ATGTCCTTTG TATGTTCATA TTACAACATG TATGCACACG 
TTAAATCTAC ATCTAATGCA AAAAGATAAA AATAAATGCA TGTTTTTGTT TGTAGAAATG 
CTGGGTACTT CAGGGGTGAA AATCCACCCA AATGGCAACA GCAACCAGTT AGGAGTCCAG 
CTAGAAAATG TGAAACTGAC AAGTTTATTC AAAAAACTTG ATAAGCGCTG CTCACTCGCC 
TCCTGGATTA AAGAAAACAT CAAGAAGAAA GAGTGCTGCT TCTATGTGGA GGATGGCAGG 
GAGGGGATTT GTAAGTGTGG TTACCCGAAG GTCCAGCACT GCGATGAGGC CATCAAACCA 
GAGGACTATA TGGGGGAACA GTGGGACAAA CACAGGCACG TCAGAGAGAC ACCCACCGAT 
GCCTTTGGAG ATATTAGTTT TGGAGGACTT GGACAGAAAA CCGGGAAGTA TGTCCGGGTG 
TCTTCTGACA CGTCTTGTGA GAATCTGTAC CAGTTGATGA CAGAACAGTG GAAACTGCGT 
TCACCAAACC TGCTGATTTC CGTGACCGGC GGAGCCAAAA ACTTCTACAT CAAGACGCAC 
CTGAAGGACA AGTTCCGCCG CGGCCTCATA AAAGTCGCCC AGACCACAGG TGCGTGGATC 
CTGACGGGAG GAACTCATGC GGGTGTGATG AAGCATGTGG GCATGGCTGT GAGGGATTAT 
ACACTCAGCA GTGGGTCAAT GGAGGGACAG ATCGTCGTCA TCGGTGTTGC GCCCTGGGGA 
GTCATTCACA ACCGCAGCAC ACTCATCCAT CCTGAGGGCC GTTTTCCGGC GTATTACTCT 
CTGGATGAGC AGGGTCAGGG CCGTCTCTCC TGCCTCGACA TCAATCACAC TCACTTCCTG 
CTGGTGGATG ATGGCACTCA AGGACATTAT GGGGTTGAGA TTGAGCTGCG GGCCCGTCTG 
GAGAAACTTA TCTCTAAACT ATCACTCGGA AATAGAGAGA GTGGAGTGAC GATACCAGTG 
GTCTGTGTGG TTCTGGACGG CGGTCCGGGA ACTCTAAATA CGATCTATAA TTCGATGCTG 
AACCACACGC CGTGTGTTGT ACTGGAGGGC TCTGGGCGTC TGGCGGATGT TATCGCTCAT 
GTGGCCTCTG TGCCCGTGTC AAAGGTCACA ATGGCTCTCA TCAACCGTCT CCTGAAGAGG 
TTTTTCATGC AAGAGTACAA AAACTTCACC GAGTTGCAGA TCATTGAGTG GACTAAGAAG 
ATTCAGGACA TCTTGCGGAT GCCTCATTTG CTCACAGTAT TCCGCATCGA TGAGGATAAA 
AACTATGACG TGGATGTGGC CATTCTGCAG GCGCTGCTAA AAGCCTCCAG AAGTGATGAA 
CATGCAGGTC GGCATTGCTG GGAGAGGCAG CTGGAGCTCG CTGTGGCCTG GAACCGCGTG 
GACATCGCAG AGAGCGAGAT CTTCACCGAG GAGAGCCAGT GGACGTCCAG TGATCTGCAT 
CCAGCCATGT TCTCCGCTCT GGTTGGTGAT AAGCCCGAGT TTGTGCGCCT CCTGCTGGAG 
AACGGTGTGT GTGTGCGTGA GTTTCTGGAG CGTGAGGAGA CTCTCTGCGA GCTCTACTCT 
CATCTGCCCT CCTGCTTCTT CCTTCGCAAA TTGGCCAAAC GGGTCCAGGG CGGAAAGATG 
CGGCGCGGGC AGGAGCCACT GCCCGGCAGC AGGAAGGTCT GCCTGAGTCA CGTCTCTGAA 
GAAGTGCGAC ACCTGCTGGG CAGCTTCACA CAACCGCTGT ACATTGCTTC TCGATACAAG 
CCCACTAAAG ATGATGTGCG CTTAAAGGTG CCGTCTAAAG GTGCTCTGGA TCTTCCATGT 
TCGGGTGAGG AATGGAGCGC AGACACAGTT TGGGATCCTG GACGGGATCT GTTCCTCTGG 
GCTGTAGTGC AGAATAACAG AGAGCTGGCG GAGATCGGTT GGGAGCAGTG CAGAGACTGT 
ATCGCTGCTG CTCTGGCCGC CAGTAAGATC TTGAGGAAGC TAGCGCAGGA GAGCGGAGAG 
GATGACAGTG AAGAGGCTAC AGAGATGCTG GAGCTCGCCA ACCACTACGA GAAACAGGCC 
ATCGGTGTGT TCAGCGAGTG CCACAGCTGG GACGCGCAGC GGGCGCAGAA GCTGCTCATC 
CGCATCTCTC CATCATGGGG CAGAAGCACA TGCCTGTGGC TCGCTCTGGA GGCTCACGAT 
AAAAGCTTCA TCGCTCACTC CGGAGTTCAG GCTCTTCTGA CTCAGATCTG GTGTGGAGAG 
CTGTCTGTAG ATAATCCACA CTGGAAAGTG CTGCTCTGCA TGATCTTCTT TCCTCTCATC 
TACACCGGCT TCCTGACCTT CAGACGGGAT GAAGACATTC AGAGACAGGC CGAACGGACG 
GAGCAGCAGA AACTGGCCAT GGAGTCTGTG TTTGCTGGAC AGTCAGACGG CAAAATCAAA 
CGTCATTTAC GTGGATTTTC ACAGAAGTCT GAGCTGAAGC CTTTGAACTG TTCGTCTCGT 
CTGATGAGTT TCCTCAAGTC TCCTCAAGTC AAGTTTTACT GGAACATCGC CTCATATTTC 
GGCTTCCTGT GGCTGTTTGC TGTGGTGCTC ATGATCGATT TCCAGACCTC GCCATCATGG 
AGGGAACTGC TGCTGTATGT GTGGCTCACG TCGCTGGTGT GTGAGGAGAT TCGACAGCTC 
TATCATGACT TTGACGGCTC TGGATTTCGG AGGAAAGCAA AGATGTACAT CAAGGATCTG 
TGGAACATTC TGGATGTGCT GTCAATCGTG CTGTTCATTG CTGGTCTAAT CTGCAGACTT 
CAGGCCTCGG ACACTGTGTT TTACATTGGT AAAGTCATCC TCTGCATCGA CTTCATCATA 
TTCTGCCTGC GATTAATGGC CATCTTCTCC ATCAGTCGAA CTCTGGGTCC CAAAATAATC 
ATCGTCAGGA GAATGATGTT GGATTTGTTT TTCTTCATGT TTCTGCTCAG TATCTGGGTT 
GTGGCGTACG GAGTAGCCAA ACAGGGCATT CTGATCGAAA ACGAAGAGAG ACTAAACTGG 
ATCATCCGTG GAGCTGTTTA TGAGCCCTAC ATCACTATAT TTGGCAACTT CCCTACTAAT 
ATAGACAATA CTCTGTTTGA TATTAGCAGC TGTAGTGTGA ACGCCTCGGA TCCTCTGAAG 
CCCAAATGTC CCATGCTGAA CGCCGATAAC ACTCCGGTGT TCCCAGAATG GCTGACCATC 
ATGATGCTGT GTGTTTATCT GCTGTTCGCC AACATCCTTT TACTCAACCT GCTCATCGCC 
ATCTTTAACT ACACGTTTCA GGAGGTTCAG GACAACACAG ACACCATCTG GAAGTTTCAG 
CGGTACGAGC TGATAAAGGA GTATCACAGC CGACCTGCTC TTCCTCCACC CTTCATCCTC 
CTAAGTCACC TCATCCTCTT CATCAGAGGA GTTTTCCTGC GTGACCTGCC GCAGAGACAC 
AAGAACTTCC GACAGGAGCT GGAGCAGACG GAGGAAGAGG AGCTGTTATC ATGGGAAGCC 
TACATGAAGG ACAACTATCT GGCATCCACA AGACAGGACG AGAGCCAGAG CGTGGAGCAT 
CGCATCCATG ACACGGCAGA GAAGGTTGGA GCGATGTCTG AACTGCTGGA GCGCGAGCAG 
GAGATGGTTT CTGCCACTAT GGCCAAACGA CTGGCCAGGC TTGAAGAGCA GGTCTCGGAG 
TCTGCCAAAG CTTTGCGATG GATTATTGAT GCGCTGAAAT CACAAGGCTG CAAATCCAAA 
GTGCAGCCTC CATTAATGAG AAGTAAAAGC TCTGATAGGG ATGATGGAGA CTCCAGCGGA 
CAGGAGACGG ATGATGAAGA GGCTCCTCAT ATGTTTGCTC GTCAGCTGCA ATATCCTGAC 
AGCACCGTCA GACGCTTTCC TGTGCCTGAG GAGAAGGTGT CCTGGGAGGT AAACTTCAGC 
CCTTATCAAC CTCCAGTTTA CAATCAACAG GACAGCAGCG AATCTGACAC TTCAGCCTTA 
GACAAGCACA GGAATCCCGG TGGCAGGACT GGGATACGAG GAAAAGGGGC TCTGAATACC 
CTTGGTCCGA ATCACATTCT CCACCCCATT TTCACCAGGT GTGGCTCTGA AATCCTGAAA 
TCTGCTCTTC TTTCAGCATC ACATGCAGGA ATTCTAATCC TATTTCAAGT GGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm2 ( XM_021478778.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334453.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X1, mRNA.

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CloneID	ODa143999
Clone ID Related Accession (Same CDS sequence)	XM_021478778.1
Accession Version	XM_021478778.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4542bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAA AAGCATGCAC ATGTCCTTTG TATGTTCATA TTACAACATG TATGCACACG 
TTAAATCTAC ATCTAATGCA AAAAGATAAA AATAAATGCA TGTTTTTGTT TGTAGAAATG 
CTGGGTACTT CAGGGGTGAA AATCCACCCA AATGGCAACA GCAACCAGTT AGGAGTCCAG 
CTAGAAAATG TGAAACTGAC AAGTTTATTC AAAAAACTTG ATAAGCGCTG CTCACTCGCC 
TCCTGGATTA AAGAAAACAT CAAGAAGAAA GAGTGCTGCT TCTATGTGGA GGATGGCAGG 
GAGGGGATTT GTAAGTGTGG TTACCCGAAG GTCCAGCACT GCGATGAGGC CATCAAACCA 
GAGGACTATA TGGGGGAACA GTGGGACAAA CACAGGCACG TCAGAGAGAC ACCCACCGAT 
GCCTTTGGAG ATATTAGTTT TGGAGGACTT GGACAGAAAA CCGGGAAGTA TGTCCGGGTG 
TCTTCTGACA CGTCTTGTGA GAATCTGTAC CAGTTGATGA CAGAACAGTG GAAACTGCGT 
TCACCAAACC TGCTGATTTC CGTGACCGGC GGAGCCAAAA ACTTCTACAT CAAGACGCAC 
CTGAAGGACA AGTTCCGCCG CGGCCTCATA AAAGTCGCCC AGACCACAGG TGCGTGGATC 
CTGACGGGAG GAACTCATGC GGGTGTGATG AAGCATGTGG GCATGGCTGT GAGGGATTAT 
ACACTCAGCA GTGGGTCAAT GGAGGGACAG ATCGTCGTCA TCGGTGTTGC GCCCTGGGGA 
GTCATTCACA ACCGCAGCAC ACTCATCCAT CCTGAGGGCC GTTTTCCGGC GTATTACTCT 
CTGGATGAGC AGGGTCAGGG CCGTCTCTCC TGCCTCGACA TCAATCACAC TCACTTCCTG 
CTGGTGGATG ATGGCACTCA AGGACATTAT GGGGTTGAGA TTGAGCTGCG GGCCCGTCTG 
GAGAAACTTA TCTCTAAACT ATCACTCGGA AATAGAGAGA GTGGAGTGAC GATACCAGTG 
GTCTGTGTGG TTCTGGACGG CGGTCCGGGA ACTCTAAATA CGATCTATAA TTCGATGCTG 
AACCACACGC CGTGTGTTGT ACTGGAGGGC TCTGGGCGTC TGGCGGATGT TATCGCTCAT 
GTGGCCTCTG TGCCCGTGTC AAAGGTCACA ATGGCTCTCA TCAACCGTCT CCTGAAGAGG 
TTTTTCATGC AAGAGTACAA AAACTTCACC GAGTTGCAGA TCATTGAGTG GACTAAGAAG 
ATTCAGGACA TCTTGCGGAT GCCTCATTTG CTCACAGTAT TCCGCATCGA TGAGGATAAA 
AACTATGACG TGGATGTGGC CATTCTGCAG GCGCTGCTAA AAGCCTCCAG AAGTGATGAA 
CATGCAGGTC GGCATTGCTG GGAGAGGCAG CTGGAGCTCG CTGTGGCCTG GAACCGCGTG 
GACATCGCAG AGAGCGAGAT CTTCACCGAG GAGAGCCAGT GGACGTCCAG TGATCTGCAT 
CCAGCCATGT TCTCCGCTCT GGTTGGTGAT AAGCCCGAGT TTGTGCGCCT CCTGCTGGAG 
AACGGTGTGT GTGTGCGTGA GTTTCTGGAG CGTGAGGAGA CTCTCTGCGA GCTCTACTCT 
CATCTGCCCT CCTGCTTCTT CCTTCGCAAA TTGGCCAAAC GGGTCCAGGG CGGAAAGATG 
CGGCGCGGGC AGGAGCCACT GCCCGGCAGC AGGAAGGTCT GCCTGAGTCA CGTCTCTGAA 
GAAGTGCGAC ACCTGCTGGG CAGCTTCACA CAACCGCTGT ACATTGCTTC TCGATACAAG 
CCCACTAAAG ATGATGTGCG CTTAAAGGTG CCGTCTAAAG GTGCTCTGGA TCTTCCATGT 
TCGGGTGAGG AATGGAGCGC AGACACAGTT TGGGATCCTG GACGGGATCT GTTCCTCTGG 
GCTGTAGTGC AGAATAACAG AGAGCTGGCG GAGATCGGTT GGGAGCAGTG CAGAGACTGT 
ATCGCTGCTG CTCTGGCCGC CAGTAAGATC TTGAGGAAGC TAGCGCAGGA GAGCGGAGAG 
GATGACAGTG AAGAGGCTAC AGAGATGCTG GAGCTCGCCA ACCACTACGA GAAACAGGCC 
ATCGGTGTGT TCAGCGAGTG CCACAGCTGG GACGCGCAGC GGGCGCAGAA GCTGCTCATC 
CGCATCTCTC CATCATGGGG CAGAAGCACA TGCCTGTGGC TCGCTCTGGA GGCTCACGAT 
AAAAGCTTCA TCGCTCACTC CGGAGTTCAG GCTCTTCTGA CTCAGATCTG GTGTGGAGAG 
CTGTCTGTAG ATAATCCACA CTGGAAAGTG CTGCTCTGCA TGATCTTCTT TCCTCTCATC 
TACACCGGCT TCCTGACCTT CAGACGGGAT GAAGACATTC AGAGACAGGC CGAACGGACG 
GAGCAGCAGA AACTGGCCAT GGAGTCTGTG TTTGCTGGAC AGTCAGACGG CAAAATCAAA 
CGTCATTTAC GTGGATTTTC ACAGAAGTCT GAGCTGAAGC CTTTGAACTG TTCGTCTCGT 
CTGATGAGTT TCCTCAAGTC TCCTCAAGTC AAGTTTTACT GGAACATCGC CTCATATTTC 
GGCTTCCTGT GGCTGTTTGC TGTGGTGCTC ATGATCGATT TCCAGACCTC GCCATCATGG 
AGGGAACTGC TGCTGTATGT GTGGCTCACG TCGCTGGTGT GTGAGGAGAT TCGACAGCTC 
TATCATGACT TTGACGGCTC TGGATTTCGG AGGAAAGCAA AGATGTACAT CAAGGATCTG 
TGGAACATTC TGGATGTGCT GTCAATCGTG CTGTTCATTG CTGGTCTAAT CTGCAGACTT 
CAGGCCTCGG ACACTGTGTT TTACATTGGT AAAGTCATCC TCTGCATCGA CTTCATCATA 
TTCTGCCTGC GATTAATGGC CATCTTCTCC ATCAGTCGAA CTCTGGGTCC CAAAATAATC 
ATCGTCAGGA GAATGATGTT GGATTTGTTT TTCTTCATGT TTCTGCTCAG TATCTGGGTT 
GTGGCGTACG GAGTAGCCAA ACAGGGCATT CTGATCGAAA ACGAAGAGAG ACTAAACTGG 
ATCATCCGTG GAGCTGTTTA TGAGCCCTAC ATCACTATAT TTGGCAACTT CCCTACTAAT 
ATAGACAATA CTCTGTTTGA TATTAGCAGC TGTAGTGTGA ACGCCTCGGA TCCTCTGAAG 
CCCAAATGTC CCATGCTGAA CGCCGATAAC ACTCCGGTGT TCCCAGAATG GCTGACCATC 
ATGATGCTGT GTGTTTATCT GCTGTTCGCC AACATCCTTT TACTCAACCT GCTCATCGCC 
ATCTTTAACT ACACGTTTCA GGAGGTTCAG GACAACACAG ACACCATCTG GAAGTTTCAG 
CGGTACGAGC TGATAAAGGA GTATCACAGC CGACCTGCTC TTCCTCCACC CTTCATCCTC 
CTAAGTCACC TCATCCTCTT CATCAGAGGA GTTTTCCTGC GTGACCTGCC GCAGAGACAC 
AAGAACTTCC GACAGGAGCT GGAGCAGACG GAGGAAGAGG AGCTGTTATC ATGGGAAGCC 
TACATGAAGG ACAACTATCT GGCATCCACA AGACAGGACG AGAGCCAGAG CGTGGAGCAT 
CGCATCCATG ACACGGCAGA GAAGGTTGGA GCGATGTCTG AACTGCTGGA GCGCGAGCAG 
GAGATGGTTT CTGCCACTAT GGCCAAACGA CTGGCCAGGC TTGAAGAGCA GGTCTCGGAG 
TCTGCCAAAG CTTTGCGATG GATTATTGAT GCGCTGAAAT CACAAGGCTG CAAATCCAAA 
GTGCAGCCTC CATTAATGAG AAGTAAAAGC TCTGATAGGG ATGATGGAGA CTCCAGCGGA 
CAGGAGACGG ATGATGAAGA GGCTCCTCAT ATGTTTGCTC GTCAGCTGCA ATATCCTGAC 
AGCACCGTCA GACGCTTTCC TGTGCCTGAG GAGAAGGTGT CCTGGGAGGT AAACTTCAGC 
CCTTATCAAC CTCCAGTTTA CAATCAACAG GACAGCAGCG AATCTGACAC TTCAGCCTTA 
GACAAGCACA GGAATCCCGG TGGCAGGACT GGGATACGAG GAAAAGGGGC TCTGAATACC 
CTTGGTCCGA ATCACATTCT CCACCCCATT TTCACCAGGT GGCGTGATGC TGAACACAAA 
GTGCTGGAGT TTCTGGCCGT TTGGGAGGAT GCAGAGAAGC GCTGGGCTCT TCTTGGGGGT 
CCAGCCCAAC CCGATGAGCC TCTAGCGCAG GTGCTGGAGA GGATTCTGGG TAAAAAGTTA 
AATGAAAAGA CCAAGACTCT TCTAAAAGCT GGAGAAGAGG TATATAAGGG TTATGTGGAT 
GACAGCAGGA ACACTGATAA CGCCTGGGTG GAGACCAGCA TCATCACACT TCACTGTGAC 
AAAAACACAC CGCTGATGGC AGATCTCAAC CACATGGTCG AGAGCTCGCT GTCGTCTCAT 
CAGCCACTCC AGTGGCGAGA AGTGAGCAGT GACGCATGTA GGTGTTCATA CCAACGAGAA 
GCTCTCCGGC AAATAGCACA TCATCACAAC ACGTACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334453.1
CDS	69..4610
Translation	MAKKACTCPLYVHITTCMHTLNLHLMQKDKNKCMFLFVEMLGTSGVKIHPNGNSNQLGVQLENVKLTSLFKKLDKRCSLASWIKENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDILRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLLIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILLSHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRWRDAEHKVLEFLAVWEDAEKRWALLGGPAQPDEPLAQVLERILGKKLNEKTKTLLKAGEEVYKGYVDDSRNTDNAWVETSIITLHCDKNTPLMADLNHMVESSLSSHQPLQWREVSSDACRCSYQREALRQIAHHHNTYF

Target ORF information:

RefSeq Version	XM_021478778.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478778.1

ORF Insert Sequence:

ATGGCAAAAA AAGCATGCAC ATGTCCTTTG TATGTTCATA TTACAACATG TATGCACACG 
TTAAATCTAC ATCTAATGCA AAAAGATAAA AATAAATGCA TGTTTTTGTT TGTAGAAATG 
CTGGGTACTT CAGGGGTGAA AATCCACCCA AATGGCAACA GCAACCAGTT AGGAGTCCAG 
CTAGAAAATG TGAAACTGAC AAGTTTATTC AAAAAACTTG ATAAGCGCTG CTCACTCGCC 
TCCTGGATTA AAGAAAACAT CAAGAAGAAA GAGTGCTGCT TCTATGTGGA GGATGGCAGG 
GAGGGGATTT GTAAGTGTGG TTACCCGAAG GTCCAGCACT GCGATGAGGC CATCAAACCA 
GAGGACTATA TGGGGGAACA GTGGGACAAA CACAGGCACG TCAGAGAGAC ACCCACCGAT 
GCCTTTGGAG ATATTAGTTT TGGAGGACTT GGACAGAAAA CCGGGAAGTA TGTCCGGGTG 
TCTTCTGACA CGTCTTGTGA GAATCTGTAC CAGTTGATGA CAGAACAGTG GAAACTGCGT 
TCACCAAACC TGCTGATTTC CGTGACCGGC GGAGCCAAAA ACTTCTACAT CAAGACGCAC 
CTGAAGGACA AGTTCCGCCG CGGCCTCATA AAAGTCGCCC AGACCACAGG TGCGTGGATC 
CTGACGGGAG GAACTCATGC GGGTGTGATG AAGCATGTGG GCATGGCTGT GAGGGATTAT 
ACACTCAGCA GTGGGTCAAT GGAGGGACAG ATCGTCGTCA TCGGTGTTGC GCCCTGGGGA 
GTCATTCACA ACCGCAGCAC ACTCATCCAT CCTGAGGGCC GTTTTCCGGC GTATTACTCT 
CTGGATGAGC AGGGTCAGGG CCGTCTCTCC TGCCTCGACA TCAATCACAC TCACTTCCTG 
CTGGTGGATG ATGGCACTCA AGGACATTAT GGGGTTGAGA TTGAGCTGCG GGCCCGTCTG 
GAGAAACTTA TCTCTAAACT ATCACTCGGA AATAGAGAGA GTGGAGTGAC GATACCAGTG 
GTCTGTGTGG TTCTGGACGG CGGTCCGGGA ACTCTAAATA CGATCTATAA TTCGATGCTG 
AACCACACGC CGTGTGTTGT ACTGGAGGGC TCTGGGCGTC TGGCGGATGT TATCGCTCAT 
GTGGCCTCTG TGCCCGTGTC AAAGGTCACA ATGGCTCTCA TCAACCGTCT CCTGAAGAGG 
TTTTTCATGC AAGAGTACAA AAACTTCACC GAGTTGCAGA TCATTGAGTG GACTAAGAAG 
ATTCAGGACA TCTTGCGGAT GCCTCATTTG CTCACAGTAT TCCGCATCGA TGAGGATAAA 
AACTATGACG TGGATGTGGC CATTCTGCAG GCGCTGCTAA AAGCCTCCAG AAGTGATGAA 
CATGCAGGTC GGCATTGCTG GGAGAGGCAG CTGGAGCTCG CTGTGGCCTG GAACCGCGTG 
GACATCGCAG AGAGCGAGAT CTTCACCGAG GAGAGCCAGT GGACGTCCAG TGATCTGCAT 
CCAGCCATGT TCTCCGCTCT GGTTGGTGAT AAGCCCGAGT TTGTGCGCCT CCTGCTGGAG 
AACGGTGTGT GTGTGCGTGA GTTTCTGGAG CGTGAGGAGA CTCTCTGCGA GCTCTACTCT 
CATCTGCCCT CCTGCTTCTT CCTTCGCAAA TTGGCCAAAC GGGTCCAGGG CGGAAAGATG 
CGGCGCGGGC AGGAGCCACT GCCCGGCAGC AGGAAGGTCT GCCTGAGTCA CGTCTCTGAA 
GAAGTGCGAC ACCTGCTGGG CAGCTTCACA CAACCGCTGT ACATTGCTTC TCGATACAAG 
CCCACTAAAG ATGATGTGCG CTTAAAGGTG CCGTCTAAAG GTGCTCTGGA TCTTCCATGT 
TCGGGTGAGG AATGGAGCGC AGACACAGTT TGGGATCCTG GACGGGATCT GTTCCTCTGG 
GCTGTAGTGC AGAATAACAG AGAGCTGGCG GAGATCGGTT GGGAGCAGTG CAGAGACTGT 
ATCGCTGCTG CTCTGGCCGC CAGTAAGATC TTGAGGAAGC TAGCGCAGGA GAGCGGAGAG 
GATGACAGTG AAGAGGCTAC AGAGATGCTG GAGCTCGCCA ACCACTACGA GAAACAGGCC 
ATCGGTGTGT TCAGCGAGTG CCACAGCTGG GACGCGCAGC GGGCGCAGAA GCTGCTCATC 
CGCATCTCTC CATCATGGGG CAGAAGCACA TGCCTGTGGC TCGCTCTGGA GGCTCACGAT 
AAAAGCTTCA TCGCTCACTC CGGAGTTCAG GCTCTTCTGA CTCAGATCTG GTGTGGAGAG 
CTGTCTGTAG ATAATCCACA CTGGAAAGTG CTGCTCTGCA TGATCTTCTT TCCTCTCATC 
TACACCGGCT TCCTGACCTT CAGACGGGAT GAAGACATTC AGAGACAGGC CGAACGGACG 
GAGCAGCAGA AACTGGCCAT GGAGTCTGTG TTTGCTGGAC AGTCAGACGG CAAAATCAAA 
CGTCATTTAC GTGGATTTTC ACAGAAGTCT GAGCTGAAGC CTTTGAACTG TTCGTCTCGT 
CTGATGAGTT TCCTCAAGTC TCCTCAAGTC AAGTTTTACT GGAACATCGC CTCATATTTC 
GGCTTCCTGT GGCTGTTTGC TGTGGTGCTC ATGATCGATT TCCAGACCTC GCCATCATGG 
AGGGAACTGC TGCTGTATGT GTGGCTCACG TCGCTGGTGT GTGAGGAGAT TCGACAGCTC 
TATCATGACT TTGACGGCTC TGGATTTCGG AGGAAAGCAA AGATGTACAT CAAGGATCTG 
TGGAACATTC TGGATGTGCT GTCAATCGTG CTGTTCATTG CTGGTCTAAT CTGCAGACTT 
CAGGCCTCGG ACACTGTGTT TTACATTGGT AAAGTCATCC TCTGCATCGA CTTCATCATA 
TTCTGCCTGC GATTAATGGC CATCTTCTCC ATCAGTCGAA CTCTGGGTCC CAAAATAATC 
ATCGTCAGGA GAATGATGTT GGATTTGTTT TTCTTCATGT TTCTGCTCAG TATCTGGGTT 
GTGGCGTACG GAGTAGCCAA ACAGGGCATT CTGATCGAAA ACGAAGAGAG ACTAAACTGG 
ATCATCCGTG GAGCTGTTTA TGAGCCCTAC ATCACTATAT TTGGCAACTT CCCTACTAAT 
ATAGACAATA CTCTGTTTGA TATTAGCAGC TGTAGTGTGA ACGCCTCGGA TCCTCTGAAG 
CCCAAATGTC CCATGCTGAA CGCCGATAAC ACTCCGGTGT TCCCAGAATG GCTGACCATC 
ATGATGCTGT GTGTTTATCT GCTGTTCGCC AACATCCTTT TACTCAACCT GCTCATCGCC 
ATCTTTAACT ACACGTTTCA GGAGGTTCAG GACAACACAG ACACCATCTG GAAGTTTCAG 
CGGTACGAGC TGATAAAGGA GTATCACAGC CGACCTGCTC TTCCTCCACC CTTCATCCTC 
CTAAGTCACC TCATCCTCTT CATCAGAGGA GTTTTCCTGC GTGACCTGCC GCAGAGACAC 
AAGAACTTCC GACAGGAGCT GGAGCAGACG GAGGAAGAGG AGCTGTTATC ATGGGAAGCC 
TACATGAAGG ACAACTATCT GGCATCCACA AGACAGGACG AGAGCCAGAG CGTGGAGCAT 
CGCATCCATG ACACGGCAGA GAAGGTTGGA GCGATGTCTG AACTGCTGGA GCGCGAGCAG 
GAGATGGTTT CTGCCACTAT GGCCAAACGA CTGGCCAGGC TTGAAGAGCA GGTCTCGGAG 
TCTGCCAAAG CTTTGCGATG GATTATTGAT GCGCTGAAAT CACAAGGCTG CAAATCCAAA 
GTGCAGCCTC CATTAATGAG AAGTAAAAGC TCTGATAGGG ATGATGGAGA CTCCAGCGGA 
CAGGAGACGG ATGATGAAGA GGCTCCTCAT ATGTTTGCTC GTCAGCTGCA ATATCCTGAC 
AGCACCGTCA GACGCTTTCC TGTGCCTGAG GAGAAGGTGT CCTGGGAGGT AAACTTCAGC 
CCTTATCAAC CTCCAGTTTA CAATCAACAG GACAGCAGCG AATCTGACAC TTCAGCCTTA 
GACAAGCACA GGAATCCCGG TGGCAGGACT GGGATACGAG GAAAAGGGGC TCTGAATACC 
CTTGGTCCGA ATCACATTCT CCACCCCATT TTCACCAGGT GGCGTGATGC TGAACACAAA 
GTGCTGGAGT TTCTGGCCGT TTGGGAGGAT GCAGAGAAGC GCTGGGCTCT TCTTGGGGGT 
CCAGCCCAAC CCGATGAGCC TCTAGCGCAG GTGCTGGAGA GGATTCTGGG TAAAAAGTTA 
AATGAAAAGA CCAAGACTCT TCTAAAAGCT GGAGAAGAGG TATATAAGGG TTATGTGGAT 
GACAGCAGGA ACACTGATAA CGCCTGGGTG GAGACCAGCA TCATCACACT TCACTGTGAC 
AAAAACACAC CGCTGATGGC AGATCTCAAC CACATGGTCG AGAGCTCGCT GTCGTCTCAT 
CAGCCACTCC AGTGGCGAGA AGTGAGCAGT GACGCATGTA GGTGTTCATA CCAACGAGAA 
GCTCTCCGGC AAATAGCACA TCATCACAAC ACGTACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm2 ( NM_001288817.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001275746.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), mRNA.

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CloneID	ODa35834
Clone ID Related Accession (Same CDS sequence)	NM_001288817.1
Accession Version	NM_001288817.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4413bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from KF305307.1. ##Evidence-Data-START## Transcript exon combination :: KF305307.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAGG CAGCGCTGGA GCCGACCCTT GTGCAAACAC TCGCGGTGAG CACTGCTAAA 
GGCGGCCGAT ATCTGTCTCT GTCTCCCTCT TTCCAGCGCT GCTCACTCGC CTCCTGGATT 
AAAGAAAACA TCAAGAAGAA AGAGTGCTGC TTCTATGTGG AGGATGGCAG GGAGGGGATT 
TGTAAGTGTG GTTACCCGAA GGTCCAGCAC TGCGATGAGG CCATCAAACC AGAGGACTAT 
ATGGGGGAAC AGTGGGACAA ACACAGGCAC GTCAGAGAGA CACCCACCGA TGCCTTTGGA 
GATATTAGTT TTGGAGGACT TGGACAGAAA ACCGGGAAGT ATGTCCGGGT GTCTTCTGAC 
ACGTCTTGTG AGAATCTGTA CCAGTTGATG ACAGAACAGT GGAAACTGCG TTCACCAAAC 
CTGCTGATTT CCGTGACCGG CGGAGCCAAA AACTTCTACA TCAAGACGCA CCTGAAGGAC 
AAGTTTCGCC GCGGCCTCAT AAAAGTCGCC CAGACCACAG GTGCGTGGAT CCTGACGGGA 
GGAACTCATG CGGGTGTGAT GAAGCATGTG GGCATGGCTG TGAGGGATTA TACACTCAGC 
AGTGGGTCAA TGGAGGGACA GATCGTCGTC ATCGGTGTTG CGCCCTGGGG AGTCATTCAC 
AACCGCAGCA CACTCATCCA TCCTGAGGGC CGTTTTCCGG CGTATTACTC TCTGGATGAG 
CAGGGTCAGG GCCGTCTCTC CTGCCTCGAC ATCAATCACA CTCACTTCCT GCTGGTGGAT 
GATGGCACTC AAGGACATTA TGGGGTTGAG ATTGAGCTGC GGGCCCGTCT GGAGAAACTT 
ATCTCTAAAC TATCACTCGG AAATAGAGAG AGTGGAGTGA CGATACCAGT GGTCTGTGTG 
GTTTTGGACG GCGGTCCGGG AACTCTAAAT ACGATCTATA ATTCGATGCT GAACCACACG 
CCGTGTGTTG TACTGGAGGG CTCTGGGCGT CTGGCGGATG TTATCGCTCA TGTGGCCTCT 
GTGCCCGTGT CAAAGGTCAC AATGGCTCTC ATCAACCGTC TCCTGAAGAG GTTTTTCATG 
CAAGAGTACA AAAACTTCAC TGAGTTGCAG ATCATTGAGT GGACTAAGAA GATTCAGGAC 
ATCATGCGGA TGCCTCATTT GCTCACAGTA TTCCGCATCG ATGAGGATAA AAACTATGAC 
GTGGATGTGG CCATTCTGCA GGCGCTGCTA AAAGCCTCCA GAAGTGATGA ACATGCGGGT 
CGGCATTGCT GGGAGAGGCA GCTGGAGCTC GCTGTGGCCT GGAACCGCGT GGACATCGCA 
GAGAGCGAGA TCTTCACCGA GGAGAGCCAG TGGACGTCCA GTGATCTGCA TCCAGCCATG 
TTCTCCGCTC TGGTTGGTGA TAAGCCCGAG TTTGTGCGCC TCCTGCTGGA GAACGGTGTG 
TGTGTGCGTG AGTTTCTGGA GCGTGAGGAG ACTCTCTGCG AGCTCTACTC TCATCTGCCC 
TCCTGCTTCT TCCTTCGCAA ATTGGCCAAA CGGGTCCAGG GCGGAAAGAT GCGGCGCGGG 
CAGGAGCCAC TGCCCGGCAG CAGGAAGGTC TGCCTGAGTC ACGTCTCTGA AGAAGTGCGA 
CACCTGCTGG GCAGCTTCAC ACAACCGCTG TACATTGCTT CTCGATACAA GCCCACTAAA 
GATGATGTGC GCTTAAAGGT GCCGTCTAAA GGTGCTCTGG ATCTTCCATG TTCGGGTGAG 
GAATGGAGCG CAGACACAGT TTGGGATCCT GGACGGGACC TGTTCCTCTG GGCTGTAGTG 
CAGAATAACA GAGAGCTGGC GGAGATCGGT TGGGAGCAGT GCAGAGACTG TATCGCTGCT 
GCTCTGGCCG CCAGTAAGAT CTTGAGGAAG CTAGCGCAGG AGAGCGGAGA GGATGACAGT 
GAAGAGGCTA CAGAGATGCT GGAGCTCGCC AACCACTACG AGAAACAGGC CATCGGTGTG 
TTCAGCGAGT GCCACAGCTG GGACGCGCAG CGGGCGCAGA AGCTGTTCAT CCGCATCTCT 
CCATCATGGG GCAGAAGCAC ATGTCTGTGG CTCGCTCTGG AGGCTCATGA TAAAAGCTTC 
ATCGCTCACT CCGGAGTTCA GGCTCTTCTG ACTCAGATCT GGTGTGGAGA GCTGTCTGTA 
GATAATCCAC ACTGGAAAGT GCTGCTCTGC ATGATCTTCT TTCCTCTCAT CTACACCGGC 
TTCCTGACCT TCAGACGGGA TGAAGACATT CAGAGACAGG CCGAACGGAC GGAGCAGCAG 
AAACTGGCCA TGGAGTCTGT GTTTGCTGGA CAGTCAGACG GCAAAATCAA ACGTCATTTA 
CGTGGATTTT CACAGAAGTC TGAGCTGAAG CCTTTGAACT GTTCGTCTCG TCTGATGAGT 
TTCCTCAAGT CTCCTCAAGT CAAGTTTTAC TGGAACATCG CCTCATATTT CGGCTTCCTG 
TGGCTGTTTG CTGTGGTGCT CATGATCGAT TTCCAGACCT CGCCATCATG GAGGGAACTG 
CTGCTGTATG TGTGGCTCAC GTCGCTGGTG TGTGAGGAGA TTCGACAGCT CTATCATGAC 
TTTGACGGCT CTGGATTTCG GAGGAAAGCA AAGATGTACA TCAAGGATCT GTGGAACATT 
CTGGATGTGC TGTCAATCGT GCTGTTCATT GCTGGTCTAA TCTGCAGACT TCAGGCCTCG 
GACACTGTGT TTTACATTGG TAAAGTCATC CTCTGCATCG ACTTCATCAT ATTCTGCCTG 
CGATTAATGG CCATCTTCTC CATCAGTCGA ACTCTGGGTC CCAAAATAAT CATCGTCAGG 
AGAATGATGT TGGATTTGTT CTTCTTCATG TTTCTGCTCA GTATCTGGGT TGTGGCGTAC 
GGAGTGGCCA AACAGGGCAT TCTGATCGAA AACGAAGAGA GACTAAACTG GATCATCCGT 
GGAGCTGTTT ATGAGCCCTA CATCACTATA TTTGGCAACT TCCCTACTAA TATAGACAAT 
ACTCTGTTTG ATATTAGCAG CTGTAGTGTG AACGCCTCGG ATCCTCTGAA GCCCAAATGT 
CCCATGCTGA ACGCTGATAA CACTCCAGTG TTCCCAGAAT GGCTGACCAT CATGATGCTG 
TGTGTTTATC TGCTGTTCGC CAACATCCTT TTACTCAACC TGCTCATCGC CATCTTTAAC 
TACACGTTTC AGGAGGTTCA GGACAACACA GACACCATCT GGAAGTTTCA GCGGTACGAG 
CTGATAAAGG AGTATCACAG CCGACCTGCA CTTCCTCCAC CCTTCATCCT CATAAGTCAC 
CTCATCCTCT TCATCAGAGG AGTTTTCCTG CGTGACCTGC CGCAGAGACA CAAGAACTTC 
CGACAGGAGC TGGAGCAGAC GGAGGAAGAG GAGCTGTTAT CATGGGAAGC CTACATGAAG 
GACAACTATC TGGCATCCAC AAGACAGGAC GAGAGCCAGA GCGTGGAGCA TCGCATCCAT 
GACACGGCAG AGAAGGTTGG AGCGATGTCT GAACTGCTGG AGCGCGAGCA GGAGATGGTT 
TCTGCCACTA TGGCCAAACG ACTGGCCAGG CTTGAAGAGC AGGTCTCGGA GTCTGCCAAA 
GCTTTGCGAT GGATTATTGA TGCGCTGAAA TCACAAGGCT GCAAATCCAA AGTGCAGCCT 
CCATTAATGA GAAGTAAAAG CTCTGATAGG GATGATGGAG ACTCCAGCGG ACAGGAGACG 
GATGATGAAG AGGCTCCTCA TATGTTTGCT CGTCAGCTTC AATATCCTGA CAGCACCGTC 
AGACGCTTTC CTGTGCCTGA GGAGAAGGTG TCCTGGGAGG TAAACTTCAG CCCTTATCAA 
CCTCCAGTTT ACAATCAACA GGACAGCAGC GAATCTGACA CTTCAGCCTT AGACAAGCAC 
AGGAATCCCG GTGGCAGGAC TGGGATACGA GGAAAAGGGG CTCTGAATAC CCTTGGTCCG 
AATCACATTC TCCACCCCAT TTTCACCAGG TGGCGTGATG CTGAACACAA AGTGCTGGAG 
TTTCTGGCCA TTTGGGAGGA TGCAGAGAAG CGCTGGGCTC TTCTTGGGGG TCCAGCCAAA 
CCCGATGAGC CTCTAGCGCA GGTGCTGGAG AGGATTCTGG GTAAAAAGTT AAATGAAAAG 
ACCAAGACTC TTCTAAAAGC TGGAGAAGAG GTATATAAGG GTTATGTGGA TGACAGCAGG 
AACACTGATA ACGCCTGGGT GGAGACCAGC ATCATCACAC TTCACTGTGA CAAAAACACA 
CCGCTGATGG CAGATCTCAA CCACATGGTC GAGAGCTCGC TGTCGTCTCA TCAGCCACTC 
CAGTGGCGAG AAGTGAGCAG TGACGCATGT AGGTGTTCAT ACCAACGAGA AGCTCTCCGG 
CAAATAGCAC ATCATCACAA CACGTACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001275746.1
CDS	29..4441
Translation	MDEAALEPTLVQTLAVSTAKGGRYLSLSPSFQRCSLASWIKENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDIMRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLFIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILISHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRWRDAEHKVLEFLAIWEDAEKRWALLGGPAKPDEPLAQVLERILGKKLNEKTKTLLKAGEEVYKGYVDDSRNTDNAWVETSIITLHCDKNTPLMADLNHMVESSLSSHQPLQWREVSSDACRCSYQREALRQIAHHHNTYF

Target ORF information:

RefSeq Version	NM_001288817.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001288817.1

ORF Insert Sequence:

ATGGATGAGG CAGCGCTGGA GCCGACCCTT GTGCAAACAC TCGCGGTGAG CACTGCTAAA 
GGCGGCCGAT ATCTGTCTCT GTCTCCCTCT TTCCAGCGCT GCTCACTCGC CTCCTGGATT 
AAAGAAAACA TCAAGAAGAA AGAGTGCTGC TTCTATGTGG AGGATGGCAG GGAGGGGATT 
TGTAAGTGTG GTTACCCGAA GGTCCAGCAC TGCGATGAGG CCATCAAACC AGAGGACTAT 
ATGGGGGAAC AGTGGGACAA ACACAGGCAC GTCAGAGAGA CACCCACCGA TGCCTTTGGA 
GATATTAGTT TTGGAGGACT TGGACAGAAA ACCGGGAAGT ATGTCCGGGT GTCTTCTGAC 
ACGTCTTGTG AGAATCTGTA CCAGTTGATG ACAGAACAGT GGAAACTGCG TTCACCAAAC 
CTGCTGATTT CCGTGACCGG CGGAGCCAAA AACTTCTACA TCAAGACGCA CCTGAAGGAC 
AAGTTTCGCC GCGGCCTCAT AAAAGTCGCC CAGACCACAG GTGCGTGGAT CCTGACGGGA 
GGAACTCATG CGGGTGTGAT GAAGCATGTG GGCATGGCTG TGAGGGATTA TACACTCAGC 
AGTGGGTCAA TGGAGGGACA GATCGTCGTC ATCGGTGTTG CGCCCTGGGG AGTCATTCAC 
AACCGCAGCA CACTCATCCA TCCTGAGGGC CGTTTTCCGG CGTATTACTC TCTGGATGAG 
CAGGGTCAGG GCCGTCTCTC CTGCCTCGAC ATCAATCACA CTCACTTCCT GCTGGTGGAT 
GATGGCACTC AAGGACATTA TGGGGTTGAG ATTGAGCTGC GGGCCCGTCT GGAGAAACTT 
ATCTCTAAAC TATCACTCGG AAATAGAGAG AGTGGAGTGA CGATACCAGT GGTCTGTGTG 
GTTTTGGACG GCGGTCCGGG AACTCTAAAT ACGATCTATA ATTCGATGCT GAACCACACG 
CCGTGTGTTG TACTGGAGGG CTCTGGGCGT CTGGCGGATG TTATCGCTCA TGTGGCCTCT 
GTGCCCGTGT CAAAGGTCAC AATGGCTCTC ATCAACCGTC TCCTGAAGAG GTTTTTCATG 
CAAGAGTACA AAAACTTCAC TGAGTTGCAG ATCATTGAGT GGACTAAGAA GATTCAGGAC 
ATCATGCGGA TGCCTCATTT GCTCACAGTA TTCCGCATCG ATGAGGATAA AAACTATGAC 
GTGGATGTGG CCATTCTGCA GGCGCTGCTA AAAGCCTCCA GAAGTGATGA ACATGCGGGT 
CGGCATTGCT GGGAGAGGCA GCTGGAGCTC GCTGTGGCCT GGAACCGCGT GGACATCGCA 
GAGAGCGAGA TCTTCACCGA GGAGAGCCAG TGGACGTCCA GTGATCTGCA TCCAGCCATG 
TTCTCCGCTC TGGTTGGTGA TAAGCCCGAG TTTGTGCGCC TCCTGCTGGA GAACGGTGTG 
TGTGTGCGTG AGTTTCTGGA GCGTGAGGAG ACTCTCTGCG AGCTCTACTC TCATCTGCCC 
TCCTGCTTCT TCCTTCGCAA ATTGGCCAAA CGGGTCCAGG GCGGAAAGAT GCGGCGCGGG 
CAGGAGCCAC TGCCCGGCAG CAGGAAGGTC TGCCTGAGTC ACGTCTCTGA AGAAGTGCGA 
CACCTGCTGG GCAGCTTCAC ACAACCGCTG TACATTGCTT CTCGATACAA GCCCACTAAA 
GATGATGTGC GCTTAAAGGT GCCGTCTAAA GGTGCTCTGG ATCTTCCATG TTCGGGTGAG 
GAATGGAGCG CAGACACAGT TTGGGATCCT GGACGGGACC TGTTCCTCTG GGCTGTAGTG 
CAGAATAACA GAGAGCTGGC GGAGATCGGT TGGGAGCAGT GCAGAGACTG TATCGCTGCT 
GCTCTGGCCG CCAGTAAGAT CTTGAGGAAG CTAGCGCAGG AGAGCGGAGA GGATGACAGT 
GAAGAGGCTA CAGAGATGCT GGAGCTCGCC AACCACTACG AGAAACAGGC CATCGGTGTG 
TTCAGCGAGT GCCACAGCTG GGACGCGCAG CGGGCGCAGA AGCTGTTCAT CCGCATCTCT 
CCATCATGGG GCAGAAGCAC ATGTCTGTGG CTCGCTCTGG AGGCTCATGA TAAAAGCTTC 
ATCGCTCACT CCGGAGTTCA GGCTCTTCTG ACTCAGATCT GGTGTGGAGA GCTGTCTGTA 
GATAATCCAC ACTGGAAAGT GCTGCTCTGC ATGATCTTCT TTCCTCTCAT CTACACCGGC 
TTCCTGACCT TCAGACGGGA TGAAGACATT CAGAGACAGG CCGAACGGAC GGAGCAGCAG 
AAACTGGCCA TGGAGTCTGT GTTTGCTGGA CAGTCAGACG GCAAAATCAA ACGTCATTTA 
CGTGGATTTT CACAGAAGTC TGAGCTGAAG CCTTTGAACT GTTCGTCTCG TCTGATGAGT 
TTCCTCAAGT CTCCTCAAGT CAAGTTTTAC TGGAACATCG CCTCATATTT CGGCTTCCTG 
TGGCTGTTTG CTGTGGTGCT CATGATCGAT TTCCAGACCT CGCCATCATG GAGGGAACTG 
CTGCTGTATG TGTGGCTCAC GTCGCTGGTG TGTGAGGAGA TTCGACAGCT CTATCATGAC 
TTTGACGGCT CTGGATTTCG GAGGAAAGCA AAGATGTACA TCAAGGATCT GTGGAACATT 
CTGGATGTGC TGTCAATCGT GCTGTTCATT GCTGGTCTAA TCTGCAGACT TCAGGCCTCG 
GACACTGTGT TTTACATTGG TAAAGTCATC CTCTGCATCG ACTTCATCAT ATTCTGCCTG 
CGATTAATGG CCATCTTCTC CATCAGTCGA ACTCTGGGTC CCAAAATAAT CATCGTCAGG 
AGAATGATGT TGGATTTGTT CTTCTTCATG TTTCTGCTCA GTATCTGGGT TGTGGCGTAC 
GGAGTGGCCA AACAGGGCAT TCTGATCGAA AACGAAGAGA GACTAAACTG GATCATCCGT 
GGAGCTGTTT ATGAGCCCTA CATCACTATA TTTGGCAACT TCCCTACTAA TATAGACAAT 
ACTCTGTTTG ATATTAGCAG CTGTAGTGTG AACGCCTCGG ATCCTCTGAA GCCCAAATGT 
CCCATGCTGA ACGCTGATAA CACTCCAGTG TTCCCAGAAT GGCTGACCAT CATGATGCTG 
TGTGTTTATC TGCTGTTCGC CAACATCCTT TTACTCAACC TGCTCATCGC CATCTTTAAC 
TACACGTTTC AGGAGGTTCA GGACAACACA GACACCATCT GGAAGTTTCA GCGGTACGAG 
CTGATAAAGG AGTATCACAG CCGACCTGCA CTTCCTCCAC CCTTCATCCT CATAAGTCAC 
CTCATCCTCT TCATCAGAGG AGTTTTCCTG CGTGACCTGC CGCAGAGACA CAAGAACTTC 
CGACAGGAGC TGGAGCAGAC GGAGGAAGAG GAGCTGTTAT CATGGGAAGC CTACATGAAG 
GACAACTATC TGGCATCCAC AAGACAGGAC GAGAGCCAGA GCGTGGAGCA TCGCATCCAT 
GACACGGCAG AGAAGGTTGG AGCGATGTCT GAACTGCTGG AGCGCGAGCA GGAGATGGTT 
TCTGCCACTA TGGCCAAACG ACTGGCCAGG CTTGAAGAGC AGGTCTCGGA GTCTGCCAAA 
GCTTTGCGAT GGATTATTGA TGCGCTGAAA TCACAAGGCT GCAAATCCAA AGTGCAGCCT 
CCATTAATGA GAAGTAAAAG CTCTGATAGG GATGATGGAG ACTCCAGCGG ACAGGAGACG 
GATGATGAAG AGGCTCCTCA TATGTTTGCT CGTCAGCTTC AATATCCTGA CAGCACCGTC 
AGACGCTTTC CTGTGCCTGA GGAGAAGGTG TCCTGGGAGG TAAACTTCAG CCCTTATCAA 
CCTCCAGTTT ACAATCAACA GGACAGCAGC GAATCTGACA CTTCAGCCTT AGACAAGCAC 
AGGAATCCCG GTGGCAGGAC TGGGATACGA GGAAAAGGGG CTCTGAATAC CCTTGGTCCG 
AATCACATTC TCCACCCCAT TTTCACCAGG TGGCGTGATG CTGAACACAA AGTGCTGGAG 
TTTCTGGCCA TTTGGGAGGA TGCAGAGAAG CGCTGGGCTC TTCTTGGGGG TCCAGCCAAA 
CCCGATGAGC CTCTAGCGCA GGTGCTGGAG AGGATTCTGG GTAAAAAGTT AAATGAAAAG 
ACCAAGACTC TTCTAAAAGC TGGAGAAGAG GTATATAAGG GTTATGTGGA TGACAGCAGG 
AACACTGATA ACGCCTGGGT GGAGACCAGC ATCATCACAC TTCACTGTGA CAAAAACACA 
CCGCTGATGG CAGATCTCAA CCACATGGTC GAGAGCTCGC TGTCGTCTCA TCAGCCACTC 
CAGTGGCGAG AAGTGAGCAG TGACGCATGT AGGTGTTCAT ACCAACGAGA AGCTCTCCGG 
CAAATAGCAC ATCATCACAA CACGTACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm2 ( XM_009304992.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303267.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X1, mRNA.

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CloneID	ODa47363
Clone ID Related Accession (Same CDS sequence)	XM_009304992.2 , XM_009304992.3 , XM_009304991.2
Accession Version	XM_009304992.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304992.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGGCGTGA TGCTGAACAC 
AAAGTGCTGG AGTTTCTGGC CGTTTGGGAG GATGCAGAGA AGCGCTGGGC TCTTCTTGGG 
GGTCCAGCCC AACCCGATGA GCCTCTAGCG CAGGTGCTGG AGAGGATTCT GGGTAAAAAG 
TTAAATGAAA AGACCAAGAC TCTTCTAAAA GCTGGAGAAG AGGTATATAA GGGTTATGTG 
GATGACAGCA GGAACACTGA TAACGCCTGG GTGGAGACCA GCATCATCAC ACTTCACTGT 
GACAAAAACA CACCGCTGAT GGCAGATCTC AACCACATGG TCGAGAGCTC GCTGTCGTCT 
CATCAGCCAC TCCAGTGGCG AGAAGTGAGC AGTGACGCAT GTAGGTGTTC ATACCAACGA 
GAAGCTCTCC GGCAAATAGC ACATCATCAC AACACGTACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303267.1
CDS	182..4606
Translation	MLGTSGVKIHPNGNSNQLGVQLENVKLTSLFKKLDKRCSLASWIKENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDILRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLLIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILLSHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRWRDAEHKVLEFLAVWEDAEKRWALLGGPAQPDEPLAQVLERILGKKLNEKTKTLLKAGEEVYKGYVDDSRNTDNAWVETSIITLHCDKNTPLMADLNHMVESSLSSHQPLQWREVSSDACRCSYQREALRQIAHHHNTYF

Target ORF information:

RefSeq Version	XM_009304992.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304992.2

ORF Insert Sequence:

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGGCGTGA TGCTGAACAC 
AAAGTGCTGG AGTTTCTGGC CGTTTGGGAG GATGCAGAGA AGCGCTGGGC TCTTCTTGGG 
GGTCCAGCCC AACCCGATGA GCCTCTAGCG CAGGTGCTGG AGAGGATTCT GGGTAAAAAG 
TTAAATGAAA AGACCAAGAC TCTTCTAAAA GCTGGAGAAG AGGTATATAA GGGTTATGTG 
GATGACAGCA GGAACACTGA TAACGCCTGG GTGGAGACCA GCATCATCAC ACTTCACTGT 
GACAAAAACA CACCGCTGAT GGCAGATCTC AACCACATGG TCGAGAGCTC GCTGTCGTCT 
CATCAGCCAC TCCAGTGGCG AGAAGTGAGC AGTGACGCAT GTAGGTGTTC ATACCAACGA 
GAAGCTCTCC GGCAAATAGC ACATCATCAC AACACGTACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm2 ( XM_009304992.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303267.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa47363
Clone ID Related Accession (Same CDS sequence)	XM_009304992.2 , XM_009304992.3 , XM_009304991.2
Accession Version	XM_009304992.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	transient receptor potential cation channel subfamily M member 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304992.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGGCGTGA TGCTGAACAC 
AAAGTGCTGG AGTTTCTGGC CGTTTGGGAG GATGCAGAGA AGCGCTGGGC TCTTCTTGGG 
GGTCCAGCCC AACCCGATGA GCCTCTAGCG CAGGTGCTGG AGAGGATTCT GGGTAAAAAG 
TTAAATGAAA AGACCAAGAC TCTTCTAAAA GCTGGAGAAG AGGTATATAA GGGTTATGTG 
GATGACAGCA GGAACACTGA TAACGCCTGG GTGGAGACCA GCATCATCAC ACTTCACTGT 
GACAAAAACA CACCGCTGAT GGCAGATCTC AACCACATGG TCGAGAGCTC GCTGTCGTCT 
CATCAGCCAC TCCAGTGGCG AGAAGTGAGC AGTGACGCAT GTAGGTGTTC ATACCAACGA 
GAAGCTCTCC GGCAAATAGC ACATCATCAC AACACGTACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303267.1
CDS	200..4624
Translation	MLGTSGVKIHPNGNSNQLGVQLENVKLTSLFKKLDKRCSLASWIKENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDILRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLLIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILLSHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRWRDAEHKVLEFLAVWEDAEKRWALLGGPAQPDEPLAQVLERILGKKLNEKTKTLLKAGEEVYKGYVDDSRNTDNAWVETSIITLHCDKNTPLMADLNHMVESSLSSHQPLQWREVSSDACRCSYQREALRQIAHHHNTYF

Target ORF information:

RefSeq Version	XM_009304992.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304992.3

ORF Insert Sequence:

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGGCGTGA TGCTGAACAC 
AAAGTGCTGG AGTTTCTGGC CGTTTGGGAG GATGCAGAGA AGCGCTGGGC TCTTCTTGGG 
GGTCCAGCCC AACCCGATGA GCCTCTAGCG CAGGTGCTGG AGAGGATTCT GGGTAAAAAG 
TTAAATGAAA AGACCAAGAC TCTTCTAAAA GCTGGAGAAG AGGTATATAA GGGTTATGTG 
GATGACAGCA GGAACACTGA TAACGCCTGG GTGGAGACCA GCATCATCAC ACTTCACTGT 
GACAAAAACA CACCGCTGAT GGCAGATCTC AACCACATGG TCGAGAGCTC GCTGTCGTCT 
CATCAGCCAC TCCAGTGGCG AGAAGTGAGC AGTGACGCAT GTAGGTGTTC ATACCAACGA 
GAAGCTCTCC GGCAAATAGC ACATCATCAC AACACGTACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm2 ( XM_009304991.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303266.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X2, mRNA.

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CloneID	ODa47363
Clone ID Related Accession (Same CDS sequence)	XM_009304992.2 , XM_009304992.3 , XM_009304991.2
Accession Version	XM_009304991.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4425bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304991.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGGCGTGA TGCTGAACAC 
AAAGTGCTGG AGTTTCTGGC CGTTTGGGAG GATGCAGAGA AGCGCTGGGC TCTTCTTGGG 
GGTCCAGCCC AACCCGATGA GCCTCTAGCG CAGGTGCTGG AGAGGATTCT GGGTAAAAAG 
TTAAATGAAA AGACCAAGAC TCTTCTAAAA GCTGGAGAAG AGGTATATAA GGGTTATGTG 
GATGACAGCA GGAACACTGA TAACGCCTGG GTGGAGACCA GCATCATCAC ACTTCACTGT 
GACAAAAACA CACCGCTGAT GGCAGATCTC AACCACATGG TCGAGAGCTC GCTGTCGTCT 
CATCAGCCAC TCCAGTGGCG AGAAGTGAGC AGTGACGCAT GTAGGTGTTC ATACCAACGA 
GAAGCTCTCC GGCAAATAGC ACATCATCAC AACACGTACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303266.1
CDS	192..4616
Translation	MLGTSGVKIHPNGNSNQLGVQLENVKLTSLFKKLDKRCSLASWIKENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDILRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLLIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILLSHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRWRDAEHKVLEFLAVWEDAEKRWALLGGPAQPDEPLAQVLERILGKKLNEKTKTLLKAGEEVYKGYVDDSRNTDNAWVETSIITLHCDKNTPLMADLNHMVESSLSSHQPLQWREVSSDACRCSYQREALRQIAHHHNTYF

Target ORF information:

RefSeq Version	XM_009304991.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304991.2

ORF Insert Sequence:

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGGCGTGA TGCTGAACAC 
AAAGTGCTGG AGTTTCTGGC CGTTTGGGAG GATGCAGAGA AGCGCTGGGC TCTTCTTGGG 
GGTCCAGCCC AACCCGATGA GCCTCTAGCG CAGGTGCTGG AGAGGATTCT GGGTAAAAAG 
TTAAATGAAA AGACCAAGAC TCTTCTAAAA GCTGGAGAAG AGGTATATAA GGGTTATGTG 
GATGACAGCA GGAACACTGA TAACGCCTGG GTGGAGACCA GCATCATCAC ACTTCACTGT 
GACAAAAACA CACCGCTGAT GGCAGATCTC AACCACATGG TCGAGAGCTC GCTGTCGTCT 
CATCAGCCAC TCCAGTGGCG AGAAGTGAGC AGTGACGCAT GTAGGTGTTC ATACCAACGA 
GAAGCTCTCC GGCAAATAGC ACATCATCAC AACACGTACT TCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm2 ( XM_017357728.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213217.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X3, mRNA.

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CloneID	ODa47364
Clone ID Related Accession (Same CDS sequence)	XM_017357728.1
Accession Version	XM_017357728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4173bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGGAAC AGTGGGACAA ACACAGGCAC GTCAGAGAGA CACCCACCGA TGCCTTTGGA 
GATATTAGTT TTGGAGGACT TGGACAGAAA ACCGGGAAGT ATGTCCGGGT GTCTTCTGAC 
ACGTCTTGTG AGAATCTGTA CCAGTTGATG ACAGAACAGT GGAAACTGCG TTCACCAAAC 
CTGCTGATTT CCGTGACCGG CGGAGCCAAA AACTTCTACA TCAAGACGCA CCTGAAGGAC 
AAGTTCCGCC GCGGCCTCAT AAAAGTCGCC CAGACCACAG GTGCGTGGAT CCTGACGGGA 
GGAACTCATG CGGGTGTGAT GAAGCATGTG GGCATGGCTG TGAGGGATTA TACACTCAGC 
AGTGGGTCAA TGGAGGGACA GATCGTCGTC ATCGGTGTTG CGCCCTGGGG AGTCATTCAC 
AACCGCAGCA CACTCATCCA TCCTGAGGGC CGTTTTCCGG CGTATTACTC TCTGGATGAG 
CAGGGTCAGG GCCGTCTCTC CTGCCTCGAC ATCAATCACA CTCACTTCCT GCTGGTGGAT 
GATGGCACTC AAGGACATTA TGGGGTTGAG ATTGAGCTGC GGGCCCGTCT GGAGAAACTT 
ATCTCTAAAC TATCACTCGG AAATAGAGAG AGTGGAGTGA CGATACCAGT GGTCTGTGTG 
GTTCTGGACG GCGGTCCGGG AACTCTAAAT ACGATCTATA ATTCGATGCT GAACCACACG 
CCGTGTGTTG TACTGGAGGG CTCTGGGCGT CTGGCGGATG TTATCGCTCA TGTGGCCTCT 
GTGCCCGTGT CAAAGGTCAC AATGGCTCTC ATCAACCGTC TCCTGAAGAG GTTTTTCATG 
CAAGAGTACA AAAACTTCAC CGAGTTGCAG ATCATTGAGT GGACTAAGAA GATTCAGGAC 
ATCTTGCGGA TGCCTCATTT GCTCACAGTA TTCCGCATCG ATGAGGATAA AAACTATGAC 
GTGGATGTGG CCATTCTGCA GGCGCTGCTA AAAGCCTCCA GAAGTGATGA ACATGCAGGT 
CGGCATTGCT GGGAGAGGCA GCTGGAGCTC GCTGTGGCCT GGAACCGCGT GGACATCGCA 
GAGAGCGAGA TCTTCACCGA GGAGAGCCAG TGGACGTCCA GTGATCTGCA TCCAGCCATG 
TTCTCCGCTC TGGTTGGTGA TAAGCCCGAG TTTGTGCGCC TCCTGCTGGA GAACGGTGTG 
TGTGTGCGTG AGTTTCTGGA GCGTGAGGAG ACTCTCTGCG AGCTCTACTC TCATCTGCCC 
TCCTGCTTCT TCCTTCGCAA ATTGGCCAAA CGGGTCCAGG GCGGAAAGAT GCGGCGCGGG 
CAGGAGCCAC TGCCCGGCAG CAGGAAGGTC TGCCTGAGTC ACGTCTCTGA AGAAGTGCGA 
CACCTGCTGG GCAGCTTCAC ACAACCGCTG TACATTGCTT CTCGATACAA GCCCACTAAA 
GATGATGTGC GCTTAAAGGT GCCGTCTAAA GGTGCTCTGG ATCTTCCATG TTCGGGTGAG 
GAATGGAGCG CAGACACAGT TTGGGATCCT GGACGGGATC TGTTCCTCTG GGCTGTAGTG 
CAGAATAACA GAGAGCTGGC GGAGATCGGT TGGGAGCAGT GCAGAGACTG TATCGCTGCT 
GCTCTGGCCG CCAGTAAGAT CTTGAGGAAG CTAGCGCAGG AGAGCGGAGA GGATGACAGT 
GAAGAGGCTA CAGAGATGCT GGAGCTCGCC AACCACTACG AGAAACAGGC CATCGGTGTG 
TTCAGCGAGT GCCACAGCTG GGACGCGCAG CGGGCGCAGA AGCTGCTCAT CCGCATCTCT 
CCATCATGGG GCAGAAGCAC ATGCCTGTGG CTCGCTCTGG AGGCTCACGA TAAAAGCTTC 
ATCGCTCACT CCGGAGTTCA GGCTCTTCTG ACTCAGATCT GGTGTGGAGA GCTGTCTGTA 
GATAATCCAC ACTGGAAAGT GCTGCTCTGC ATGATCTTCT TTCCTCTCAT CTACACCGGC 
TTCCTGACCT TCAGACGGGA TGAAGACATT CAGAGACAGG CCGAACGGAC GGAGCAGCAG 
AAACTGGCCA TGGAGTCTGT GTTTGCTGGA CAGTCAGACG GCAAAATCAA ACGTCATTTA 
CGTGGATTTT CACAGAAGTC TGAGCTGAAG CCTTTGAACT GTTCGTCTCG TCTGATGAGT 
TTCCTCAAGT CTCCTCAAGT CAAGTTTTAC TGGAACATCG CCTCATATTT CGGCTTCCTG 
TGGCTGTTTG CTGTGGTGCT CATGATCGAT TTCCAGACCT CGCCATCATG GAGGGAACTG 
CTGCTGTATG TGTGGCTCAC GTCGCTGGTG TGTGAGGAGA TTCGACAGCT CTATCATGAC 
TTTGACGGCT CTGGATTTCG GAGGAAAGCA AAGATGTACA TCAAGGATCT GTGGAACATT 
CTGGATGTGC TGTCAATCGT GCTGTTCATT GCTGGTCTAA TCTGCAGACT TCAGGCCTCG 
GACACTGTGT TTTACATTGG TAAAGTCATC CTCTGCATCG ACTTCATCAT ATTCTGCCTG 
CGATTAATGG CCATCTTCTC CATCAGTCGA ACTCTGGGTC CCAAAATAAT CATCGTCAGG 
AGAATGATGT TGGATTTGTT TTTCTTCATG TTTCTGCTCA GTATCTGGGT TGTGGCGTAC 
GGAGTAGCCA AACAGGGCAT TCTGATCGAA AACGAAGAGA GACTAAACTG GATCATCCGT 
GGAGCTGTTT ATGAGCCCTA CATCACTATA TTTGGCAACT TCCCTACTAA TATAGACAAT 
ACTCTGTTTG ATATTAGCAG CTGTAGTGTG AACGCCTCGG ATCCTCTGAA GCCCAAATGT 
CCCATGCTGA ACGCCGATAA CACTCCGGTG TTCCCAGAAT GGCTGACCAT CATGATGCTG 
TGTGTTTATC TGCTGTTCGC CAACATCCTT TTACTCAACC TGCTCATCGC CATCTTTAAC 
TACACGTTTC AGGAGGTTCA GGACAACACA GACACCATCT GGAAGTTTCA GCGGTACGAG 
CTGATAAAGG AGTATCACAG CCGACCTGCT CTTCCTCCAC CCTTCATCCT CCTAAGTCAC 
CTCATCCTCT TCATCAGAGG AGTTTTCCTG CGTGACCTGC CGCAGAGACA CAAGAACTTC 
CGACAGGAGC TGGAGCAGAC GGAGGAAGAG GAGCTGTTAT CATGGGAAGC CTACATGAAG 
GACAACTATC TGGCATCCAC AAGACAGGAC GAGAGCCAGA GCGTGGAGCA TCGCATCCAT 
GACACGGCAG AGAAGGTTGG AGCGATGTCT GAACTGCTGG AGCGCGAGCA GGAGATGGTT 
TCTGCCACTA TGGCCAAACG ACTGGCCAGG CTTGAAGAGC AGGTCTCGGA GTCTGCCAAA 
GCTTTGCGAT GGATTATTGA TGCGCTGAAA TCACAAGGCT GCAAATCCAA AGTGCAGCCT 
CCATTAATGA GAAGTAAAAG CTCTGATAGG GATGATGGAG ACTCCAGCGG ACAGGAGACG 
GATGATGAAG AGGCTCCTCA TATGTTTGCT CGTCAGCTGC AATATCCTGA CAGCACCGTC 
AGACGCTTTC CTGTGCCTGA GGAGAAGGTG TCCTGGGAGG TAAACTTCAG CCCTTATCAA 
CCTCCAGTTT ACAATCAACA GGACAGCAGC GAATCTGACA CTTCAGCCTT AGACAAGCAC 
AGGAATCCCG GTGGCAGGAC TGGGATACGA GGAAAAGGGG CTCTGAATAC CCTTGGTCCG 
AATCACATTC TCCACCCCAT TTTCACCAGG TGGCGTGATG CTGAACACAA AGTGCTGGAG 
TTTCTGGCCG TTTGGGAGGA TGCAGAGAAG CGCTGGGCTC TTCTTGGGGG TCCAGCCCAA 
CCCGATGAGC CTCTAGCGCA GGTGCTGGAG AGGATTCTGG GTAAAAAGTT AAATGAAAAG 
ACCAAGACTC TTCTAAAAGC TGGAGAAGAG GTATATAAGG GTTATGTGGA TGACAGCAGG 
AACACTGATA ACGCCTGGGT GGAGACCAGC ATCATCACAC TTCACTGTGA CAAAAACACA 
CCGCTGATGG CAGATCTCAA CCACATGGTC GAGAGCTCGC TGTCGTCTCA TCAGCCACTC 
CAGTGGCGAG AAGTGAGCAG TGACGCATGT AGGTGTTCAT ACCAACGAGA AGCTCTCCGG 
CAAATAGCAC ATCATCACAA CACGTACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213217.1
CDS	324..4496
Translation	MGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDILRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLLIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILLSHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRWRDAEHKVLEFLAVWEDAEKRWALLGGPAQPDEPLAQVLERILGKKLNEKTKTLLKAGEEVYKGYVDDSRNTDNAWVETSIITLHCDKNTPLMADLNHMVESSLSSHQPLQWREVSSDACRCSYQREALRQIAHHHNTYF

Target ORF information:

RefSeq Version	XM_017357728.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357728.1

ORF Insert Sequence:

ATGGGGGAAC AGTGGGACAA ACACAGGCAC GTCAGAGAGA CACCCACCGA TGCCTTTGGA 
GATATTAGTT TTGGAGGACT TGGACAGAAA ACCGGGAAGT ATGTCCGGGT GTCTTCTGAC 
ACGTCTTGTG AGAATCTGTA CCAGTTGATG ACAGAACAGT GGAAACTGCG TTCACCAAAC 
CTGCTGATTT CCGTGACCGG CGGAGCCAAA AACTTCTACA TCAAGACGCA CCTGAAGGAC 
AAGTTCCGCC GCGGCCTCAT AAAAGTCGCC CAGACCACAG GTGCGTGGAT CCTGACGGGA 
GGAACTCATG CGGGTGTGAT GAAGCATGTG GGCATGGCTG TGAGGGATTA TACACTCAGC 
AGTGGGTCAA TGGAGGGACA GATCGTCGTC ATCGGTGTTG CGCCCTGGGG AGTCATTCAC 
AACCGCAGCA CACTCATCCA TCCTGAGGGC CGTTTTCCGG CGTATTACTC TCTGGATGAG 
CAGGGTCAGG GCCGTCTCTC CTGCCTCGAC ATCAATCACA CTCACTTCCT GCTGGTGGAT 
GATGGCACTC AAGGACATTA TGGGGTTGAG ATTGAGCTGC GGGCCCGTCT GGAGAAACTT 
ATCTCTAAAC TATCACTCGG AAATAGAGAG AGTGGAGTGA CGATACCAGT GGTCTGTGTG 
GTTCTGGACG GCGGTCCGGG AACTCTAAAT ACGATCTATA ATTCGATGCT GAACCACACG 
CCGTGTGTTG TACTGGAGGG CTCTGGGCGT CTGGCGGATG TTATCGCTCA TGTGGCCTCT 
GTGCCCGTGT CAAAGGTCAC AATGGCTCTC ATCAACCGTC TCCTGAAGAG GTTTTTCATG 
CAAGAGTACA AAAACTTCAC CGAGTTGCAG ATCATTGAGT GGACTAAGAA GATTCAGGAC 
ATCTTGCGGA TGCCTCATTT GCTCACAGTA TTCCGCATCG ATGAGGATAA AAACTATGAC 
GTGGATGTGG CCATTCTGCA GGCGCTGCTA AAAGCCTCCA GAAGTGATGA ACATGCAGGT 
CGGCATTGCT GGGAGAGGCA GCTGGAGCTC GCTGTGGCCT GGAACCGCGT GGACATCGCA 
GAGAGCGAGA TCTTCACCGA GGAGAGCCAG TGGACGTCCA GTGATCTGCA TCCAGCCATG 
TTCTCCGCTC TGGTTGGTGA TAAGCCCGAG TTTGTGCGCC TCCTGCTGGA GAACGGTGTG 
TGTGTGCGTG AGTTTCTGGA GCGTGAGGAG ACTCTCTGCG AGCTCTACTC TCATCTGCCC 
TCCTGCTTCT TCCTTCGCAA ATTGGCCAAA CGGGTCCAGG GCGGAAAGAT GCGGCGCGGG 
CAGGAGCCAC TGCCCGGCAG CAGGAAGGTC TGCCTGAGTC ACGTCTCTGA AGAAGTGCGA 
CACCTGCTGG GCAGCTTCAC ACAACCGCTG TACATTGCTT CTCGATACAA GCCCACTAAA 
GATGATGTGC GCTTAAAGGT GCCGTCTAAA GGTGCTCTGG ATCTTCCATG TTCGGGTGAG 
GAATGGAGCG CAGACACAGT TTGGGATCCT GGACGGGATC TGTTCCTCTG GGCTGTAGTG 
CAGAATAACA GAGAGCTGGC GGAGATCGGT TGGGAGCAGT GCAGAGACTG TATCGCTGCT 
GCTCTGGCCG CCAGTAAGAT CTTGAGGAAG CTAGCGCAGG AGAGCGGAGA GGATGACAGT 
GAAGAGGCTA CAGAGATGCT GGAGCTCGCC AACCACTACG AGAAACAGGC CATCGGTGTG 
TTCAGCGAGT GCCACAGCTG GGACGCGCAG CGGGCGCAGA AGCTGCTCAT CCGCATCTCT 
CCATCATGGG GCAGAAGCAC ATGCCTGTGG CTCGCTCTGG AGGCTCACGA TAAAAGCTTC 
ATCGCTCACT CCGGAGTTCA GGCTCTTCTG ACTCAGATCT GGTGTGGAGA GCTGTCTGTA 
GATAATCCAC ACTGGAAAGT GCTGCTCTGC ATGATCTTCT TTCCTCTCAT CTACACCGGC 
TTCCTGACCT TCAGACGGGA TGAAGACATT CAGAGACAGG CCGAACGGAC GGAGCAGCAG 
AAACTGGCCA TGGAGTCTGT GTTTGCTGGA CAGTCAGACG GCAAAATCAA ACGTCATTTA 
CGTGGATTTT CACAGAAGTC TGAGCTGAAG CCTTTGAACT GTTCGTCTCG TCTGATGAGT 
TTCCTCAAGT CTCCTCAAGT CAAGTTTTAC TGGAACATCG CCTCATATTT CGGCTTCCTG 
TGGCTGTTTG CTGTGGTGCT CATGATCGAT TTCCAGACCT CGCCATCATG GAGGGAACTG 
CTGCTGTATG TGTGGCTCAC GTCGCTGGTG TGTGAGGAGA TTCGACAGCT CTATCATGAC 
TTTGACGGCT CTGGATTTCG GAGGAAAGCA AAGATGTACA TCAAGGATCT GTGGAACATT 
CTGGATGTGC TGTCAATCGT GCTGTTCATT GCTGGTCTAA TCTGCAGACT TCAGGCCTCG 
GACACTGTGT TTTACATTGG TAAAGTCATC CTCTGCATCG ACTTCATCAT ATTCTGCCTG 
CGATTAATGG CCATCTTCTC CATCAGTCGA ACTCTGGGTC CCAAAATAAT CATCGTCAGG 
AGAATGATGT TGGATTTGTT TTTCTTCATG TTTCTGCTCA GTATCTGGGT TGTGGCGTAC 
GGAGTAGCCA AACAGGGCAT TCTGATCGAA AACGAAGAGA GACTAAACTG GATCATCCGT 
GGAGCTGTTT ATGAGCCCTA CATCACTATA TTTGGCAACT TCCCTACTAA TATAGACAAT 
ACTCTGTTTG ATATTAGCAG CTGTAGTGTG AACGCCTCGG ATCCTCTGAA GCCCAAATGT 
CCCATGCTGA ACGCCGATAA CACTCCGGTG TTCCCAGAAT GGCTGACCAT CATGATGCTG 
TGTGTTTATC TGCTGTTCGC CAACATCCTT TTACTCAACC TGCTCATCGC CATCTTTAAC 
TACACGTTTC AGGAGGTTCA GGACAACACA GACACCATCT GGAAGTTTCA GCGGTACGAG 
CTGATAAAGG AGTATCACAG CCGACCTGCT CTTCCTCCAC CCTTCATCCT CCTAAGTCAC 
CTCATCCTCT TCATCAGAGG AGTTTTCCTG CGTGACCTGC CGCAGAGACA CAAGAACTTC 
CGACAGGAGC TGGAGCAGAC GGAGGAAGAG GAGCTGTTAT CATGGGAAGC CTACATGAAG 
GACAACTATC TGGCATCCAC AAGACAGGAC GAGAGCCAGA GCGTGGAGCA TCGCATCCAT 
GACACGGCAG AGAAGGTTGG AGCGATGTCT GAACTGCTGG AGCGCGAGCA GGAGATGGTT 
TCTGCCACTA TGGCCAAACG ACTGGCCAGG CTTGAAGAGC AGGTCTCGGA GTCTGCCAAA 
GCTTTGCGAT GGATTATTGA TGCGCTGAAA TCACAAGGCT GCAAATCCAA AGTGCAGCCT 
CCATTAATGA GAAGTAAAAG CTCTGATAGG GATGATGGAG ACTCCAGCGG ACAGGAGACG 
GATGATGAAG AGGCTCCTCA TATGTTTGCT CGTCAGCTGC AATATCCTGA CAGCACCGTC 
AGACGCTTTC CTGTGCCTGA GGAGAAGGTG TCCTGGGAGG TAAACTTCAG CCCTTATCAA 
CCTCCAGTTT ACAATCAACA GGACAGCAGC GAATCTGACA CTTCAGCCTT AGACAAGCAC 
AGGAATCCCG GTGGCAGGAC TGGGATACGA GGAAAAGGGG CTCTGAATAC CCTTGGTCCG 
AATCACATTC TCCACCCCAT TTTCACCAGG TGGCGTGATG CTGAACACAA AGTGCTGGAG 
TTTCTGGCCG TTTGGGAGGA TGCAGAGAAG CGCTGGGCTC TTCTTGGGGG TCCAGCCCAA 
CCCGATGAGC CTCTAGCGCA GGTGCTGGAG AGGATTCTGG GTAAAAAGTT AAATGAAAAG 
ACCAAGACTC TTCTAAAAGC TGGAGAAGAG GTATATAAGG GTTATGTGGA TGACAGCAGG 
AACACTGATA ACGCCTGGGT GGAGACCAGC ATCATCACAC TTCACTGTGA CAAAAACACA 
CCGCTGATGG CAGATCTCAA CCACATGGTC GAGAGCTCGC TGTCGTCTCA TCAGCCACTC 
CAGTGGCGAG AAGTGAGCAG TGACGCATGT AGGTGTTCAT ACCAACGAGA AGCTCTCCGG 
CAAATAGCAC ATCATCACAA CACGTACTTC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

trpm2 ( XM_017357729.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213218.1 Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X4, mRNA.

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CloneID	ODa47365
Clone ID Related Accession (Same CDS sequence)	XM_017357729.1
Accession Version	XM_017357729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4080bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	transient receptor potential cation channel subfamily M member 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGTGGCTC TGAAATCCTG 
AAATCTGCTC TTCTTTCAGC ATCACATGCA GGAATTCTAA TCCTATTTCA AGTGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213218.1
CDS	182..4261
Translation	MLGTSGVKIHPNGNSNQLGVQLENVKLTSLFKKLDKRCSLASWIKENIKKKECCFYVEDGREGICKCGYPKVQHCDEAIKPEDYMGEQWDKHRHVRETPTDAFGDISFGGLGQKTGKYVRVSSDTSCENLYQLMTEQWKLRSPNLLISVTGGAKNFYIKTHLKDKFRRGLIKVAQTTGAWILTGGTHAGVMKHVGMAVRDYTLSSGSMEGQIVVIGVAPWGVIHNRSTLIHPEGRFPAYYSLDEQGQGRLSCLDINHTHFLLVDDGTQGHYGVEIELRARLEKLISKLSLGNRESGVTIPVVCVVLDGGPGTLNTIYNSMLNHTPCVVLEGSGRLADVIAHVASVPVSKVTMALINRLLKRFFMQEYKNFTELQIIEWTKKIQDILRMPHLLTVFRIDEDKNYDVDVAILQALLKASRSDEHAGRHCWERQLELAVAWNRVDIAESEIFTEESQWTSSDLHPAMFSALVGDKPEFVRLLLENGVCVREFLEREETLCELYSHLPSCFFLRKLAKRVQGGKMRRGQEPLPGSRKVCLSHVSEEVRHLLGSFTQPLYIASRYKPTKDDVRLKVPSKGALDLPCSGEEWSADTVWDPGRDLFLWAVVQNNRELAEIGWEQCRDCIAAALAASKILRKLAQESGEDDSEEATEMLELANHYEKQAIGVFSECHSWDAQRAQKLLIRISPSWGRSTCLWLALEAHDKSFIAHSGVQALLTQIWCGELSVDNPHWKVLLCMIFFPLIYTGFLTFRRDEDIQRQAERTEQQKLAMESVFAGQSDGKIKRHLRGFSQKSELKPLNCSSRLMSFLKSPQVKFYWNIASYFGFLWLFAVVLMIDFQTSPSWRELLLYVWLTSLVCEEIRQLYHDFDGSGFRRKAKMYIKDLWNILDVLSIVLFIAGLICRLQASDTVFYIGKVILCIDFIIFCLRLMAIFSISRTLGPKIIIVRRMMLDLFFFMFLLSIWVVAYGVAKQGILIENEERLNWIIRGAVYEPYITIFGNFPTNIDNTLFDISSCSVNASDPLKPKCPMLNADNTPVFPEWLTIMMLCVYLLFANILLLNLLIAIFNYTFQEVQDNTDTIWKFQRYELIKEYHSRPALPPPFILLSHLILFIRGVFLRDLPQRHKNFRQELEQTEEEELLSWEAYMKDNYLASTRQDESQSVEHRIHDTAEKVGAMSELLEREQEMVSATMAKRLARLEEQVSESAKALRWIIDALKSQGCKSKVQPPLMRSKSSDRDDGDSSGQETDDEEAPHMFARQLQYPDSTVRRFPVPEEKVSWEVNFSPYQPPVYNQQDSSESDTSALDKHRNPGGRTGIRGKGALNTLGPNHILHPIFTRCGSEILKSALLSASHAGILILFQVA

Target ORF information:

RefSeq Version	XM_017357729.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio transient receptor potential cation channel, subfamily M, member 2 (trpm2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357729.1

ORF Insert Sequence:

ATGCTGGGTA CTTCAGGGGT GAAAATCCAC CCAAATGGCA ACAGCAACCA GTTAGGAGTC 
CAGCTAGAAA ATGTGAAACT GACAAGTTTA TTCAAAAAAC TTGATAAGCG CTGCTCACTC 
GCCTCCTGGA TTAAAGAAAA CATCAAGAAG AAAGAGTGCT GCTTCTATGT GGAGGATGGC 
AGGGAGGGGA TTTGTAAGTG TGGTTACCCG AAGGTCCAGC ACTGCGATGA GGCCATCAAA 
CCAGAGGACT ATATGGGGGA ACAGTGGGAC AAACACAGGC ACGTCAGAGA GACACCCACC 
GATGCCTTTG GAGATATTAG TTTTGGAGGA CTTGGACAGA AAACCGGGAA GTATGTCCGG 
GTGTCTTCTG ACACGTCTTG TGAGAATCTG TACCAGTTGA TGACAGAACA GTGGAAACTG 
CGTTCACCAA ACCTGCTGAT TTCCGTGACC GGCGGAGCCA AAAACTTCTA CATCAAGACG 
CACCTGAAGG ACAAGTTCCG CCGCGGCCTC ATAAAAGTCG CCCAGACCAC AGGTGCGTGG 
ATCCTGACGG GAGGAACTCA TGCGGGTGTG ATGAAGCATG TGGGCATGGC TGTGAGGGAT 
TATACACTCA GCAGTGGGTC AATGGAGGGA CAGATCGTCG TCATCGGTGT TGCGCCCTGG 
GGAGTCATTC ACAACCGCAG CACACTCATC CATCCTGAGG GCCGTTTTCC GGCGTATTAC 
TCTCTGGATG AGCAGGGTCA GGGCCGTCTC TCCTGCCTCG ACATCAATCA CACTCACTTC 
CTGCTGGTGG ATGATGGCAC TCAAGGACAT TATGGGGTTG AGATTGAGCT GCGGGCCCGT 
CTGGAGAAAC TTATCTCTAA ACTATCACTC GGAAATAGAG AGAGTGGAGT GACGATACCA 
GTGGTCTGTG TGGTTCTGGA CGGCGGTCCG GGAACTCTAA ATACGATCTA TAATTCGATG 
CTGAACCACA CGCCGTGTGT TGTACTGGAG GGCTCTGGGC GTCTGGCGGA TGTTATCGCT 
CATGTGGCCT CTGTGCCCGT GTCAAAGGTC ACAATGGCTC TCATCAACCG TCTCCTGAAG 
AGGTTTTTCA TGCAAGAGTA CAAAAACTTC ACCGAGTTGC AGATCATTGA GTGGACTAAG 
AAGATTCAGG ACATCTTGCG GATGCCTCAT TTGCTCACAG TATTCCGCAT CGATGAGGAT 
AAAAACTATG ACGTGGATGT GGCCATTCTG CAGGCGCTGC TAAAAGCCTC CAGAAGTGAT 
GAACATGCAG GTCGGCATTG CTGGGAGAGG CAGCTGGAGC TCGCTGTGGC CTGGAACCGC 
GTGGACATCG CAGAGAGCGA GATCTTCACC GAGGAGAGCC AGTGGACGTC CAGTGATCTG 
CATCCAGCCA TGTTCTCCGC TCTGGTTGGT GATAAGCCCG AGTTTGTGCG CCTCCTGCTG 
GAGAACGGTG TGTGTGTGCG TGAGTTTCTG GAGCGTGAGG AGACTCTCTG CGAGCTCTAC 
TCTCATCTGC CCTCCTGCTT CTTCCTTCGC AAATTGGCCA AACGGGTCCA GGGCGGAAAG 
ATGCGGCGCG GGCAGGAGCC ACTGCCCGGC AGCAGGAAGG TCTGCCTGAG TCACGTCTCT 
GAAGAAGTGC GACACCTGCT GGGCAGCTTC ACACAACCGC TGTACATTGC TTCTCGATAC 
AAGCCCACTA AAGATGATGT GCGCTTAAAG GTGCCGTCTA AAGGTGCTCT GGATCTTCCA 
TGTTCGGGTG AGGAATGGAG CGCAGACACA GTTTGGGATC CTGGACGGGA TCTGTTCCTC 
TGGGCTGTAG TGCAGAATAA CAGAGAGCTG GCGGAGATCG GTTGGGAGCA GTGCAGAGAC 
TGTATCGCTG CTGCTCTGGC CGCCAGTAAG ATCTTGAGGA AGCTAGCGCA GGAGAGCGGA 
GAGGATGACA GTGAAGAGGC TACAGAGATG CTGGAGCTCG CCAACCACTA CGAGAAACAG 
GCCATCGGTG TGTTCAGCGA GTGCCACAGC TGGGACGCGC AGCGGGCGCA GAAGCTGCTC 
ATCCGCATCT CTCCATCATG GGGCAGAAGC ACATGCCTGT GGCTCGCTCT GGAGGCTCAC 
GATAAAAGCT TCATCGCTCA CTCCGGAGTT CAGGCTCTTC TGACTCAGAT CTGGTGTGGA 
GAGCTGTCTG TAGATAATCC ACACTGGAAA GTGCTGCTCT GCATGATCTT CTTTCCTCTC 
ATCTACACCG GCTTCCTGAC CTTCAGACGG GATGAAGACA TTCAGAGACA GGCCGAACGG 
ACGGAGCAGC AGAAACTGGC CATGGAGTCT GTGTTTGCTG GACAGTCAGA CGGCAAAATC 
AAACGTCATT TACGTGGATT TTCACAGAAG TCTGAGCTGA AGCCTTTGAA CTGTTCGTCT 
CGTCTGATGA GTTTCCTCAA GTCTCCTCAA GTCAAGTTTT ACTGGAACAT CGCCTCATAT 
TTCGGCTTCC TGTGGCTGTT TGCTGTGGTG CTCATGATCG ATTTCCAGAC CTCGCCATCA 
TGGAGGGAAC TGCTGCTGTA TGTGTGGCTC ACGTCGCTGG TGTGTGAGGA GATTCGACAG 
CTCTATCATG ACTTTGACGG CTCTGGATTT CGGAGGAAAG CAAAGATGTA CATCAAGGAT 
CTGTGGAACA TTCTGGATGT GCTGTCAATC GTGCTGTTCA TTGCTGGTCT AATCTGCAGA 
CTTCAGGCCT CGGACACTGT GTTTTACATT GGTAAAGTCA TCCTCTGCAT CGACTTCATC 
ATATTCTGCC TGCGATTAAT GGCCATCTTC TCCATCAGTC GAACTCTGGG TCCCAAAATA 
ATCATCGTCA GGAGAATGAT GTTGGATTTG TTTTTCTTCA TGTTTCTGCT CAGTATCTGG 
GTTGTGGCGT ACGGAGTAGC CAAACAGGGC ATTCTGATCG AAAACGAAGA GAGACTAAAC 
TGGATCATCC GTGGAGCTGT TTATGAGCCC TACATCACTA TATTTGGCAA CTTCCCTACT 
AATATAGACA ATACTCTGTT TGATATTAGC AGCTGTAGTG TGAACGCCTC GGATCCTCTG 
AAGCCCAAAT GTCCCATGCT GAACGCCGAT AACACTCCGG TGTTCCCAGA ATGGCTGACC 
ATCATGATGC TGTGTGTTTA TCTGCTGTTC GCCAACATCC TTTTACTCAA CCTGCTCATC 
GCCATCTTTA ACTACACGTT TCAGGAGGTT CAGGACAACA CAGACACCAT CTGGAAGTTT 
CAGCGGTACG AGCTGATAAA GGAGTATCAC AGCCGACCTG CTCTTCCTCC ACCCTTCATC 
CTCCTAAGTC ACCTCATCCT CTTCATCAGA GGAGTTTTCC TGCGTGACCT GCCGCAGAGA 
CACAAGAACT TCCGACAGGA GCTGGAGCAG ACGGAGGAAG AGGAGCTGTT ATCATGGGAA 
GCCTACATGA AGGACAACTA TCTGGCATCC ACAAGACAGG ACGAGAGCCA GAGCGTGGAG 
CATCGCATCC ATGACACGGC AGAGAAGGTT GGAGCGATGT CTGAACTGCT GGAGCGCGAG 
CAGGAGATGG TTTCTGCCAC TATGGCCAAA CGACTGGCCA GGCTTGAAGA GCAGGTCTCG 
GAGTCTGCCA AAGCTTTGCG ATGGATTATT GATGCGCTGA AATCACAAGG CTGCAAATCC 
AAAGTGCAGC CTCCATTAAT GAGAAGTAAA AGCTCTGATA GGGATGATGG AGACTCCAGC 
GGACAGGAGA CGGATGATGA AGAGGCTCCT CATATGTTTG CTCGTCAGCT GCAATATCCT 
GACAGCACCG TCAGACGCTT TCCTGTGCCT GAGGAGAAGG TGTCCTGGGA GGTAAACTTC 
AGCCCTTATC AACCTCCAGT TTACAATCAA CAGGACAGCA GCGAATCTGA CACTTCAGCC 
TTAGACAAGC ACAGGAATCC CGGTGGCAGG ACTGGGATAC GAGGAAAAGG GGCTCTGAAT 
ACCCTTGGTC CGAATCACAT TCTCCACCCC ATTTTCACCA GGTGTGGCTC TGAAATCCTG 
AAATCTGCTC TTCTTTCAGC ATCACATGCA GGAATTCTAA TCCTATTTCA AGTGGCGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Thermal stress in Danio rerio: a link between temperature, light, thermo-TRP channels, and clock genes.
Journal of thermal biology68(Pt A)128-138(2017 Aug)
Jer?nimo R,Moraes MN,de Assis LVM,Ramos BC,Rocha T,Castrucci AML

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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trpm2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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