nhsl2 cDNA ORF clone, Danio rerio(zebrafish)

The following nhsl2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nhsl2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa46887 XM_009303453.2
Latest version!
Danio rerio NHS-like 2 (nhsl2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa46887 XM_021477957.1
Latest version!
Danio rerio NHS-like 2 (nhsl2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
ODa13435 XM_001334017.7
Latest version!
Danio rerio NHS-like 2 (nhsl2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46883 XM_009303449.3
Latest version!
Danio rerio NHS-like 2 (nhsl2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46884 XM_009303450.3
Latest version!
Danio rerio NHS-like 2 (nhsl2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46885 XM_009303451.3
Latest version!
Danio rerio NHS-like 2 (nhsl2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa13435 XM_001334017.6 Danio rerio NHS-like 2 (nhsl2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46883 XM_009303449.2 Danio rerio NHS-like 2 (nhsl2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46884 XM_009303450.2 Danio rerio NHS-like 2 (nhsl2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa46885 XM_009303451.2 Danio rerio NHS-like 2 (nhsl2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa46887
    Clone ID Related Accession (Same CDS sequence) XM_021477957.1 , XM_009303453.2
    Accession Version XM_021477957.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGGATC ATGCCACTCT AATGTGCCCA AGCCCTTCCT GGAATGGCCC AAAAGGTTCC 
    ACTTTCTCTC CCTCCTGGAA TGACTCCTAC AACTATGCCT TGGTTACCAG CCCTGCTTCC
    AAGCAGCCAG TGACTAAAAC TGGAATGTTA ACACCTGGAG GTGATAGGGG TGGTCTAATT
    TGTCCATCTC AGACTAGTTC AGGCCTCTCC ATGAGTTCAG GGTCACACTC CAGTTCTTTC
    ACCTCCATCT CTGAACCATG TGGGCACAGA GTATACACAG TGTCCACACA GGGGAGTGGA
    GTTGTAACAG GGAAACGGAA TGAGACTGAG GTGACAGCTT CTACTGCAGT CACAAGAAAT
    AGCAAAAGTG AGCAGGAAAA GAGATCAGCC CGATCCAATG TCTTCAGGTT CCGTGAGCGC
    TCACTTTCAA CACCAACAGA CTCTGGATCA TTTTGCTCAG CAGATAATAT ATGTTCTCCA
    GGCGAGGCTG TCTCTGGGGT TCCAGAGGGT GAAAGTTATG CCTTGCTTTA TCCGAGTAAC
    AGCTCGGAGG ATAGCACGAG CACTGATAAT GTTTCTGTGA CCACAGGTTC AGACTTTCTT
    CCTGATGGTC GACTAAGGTC ACGGTCACGC AGTATCTCAC TAAAGAAATC TAAAAAAAAG
    CCACCACCTC CTGTGCGTAG TGTATCTCTG ATGAAAAACC TATCAGAAAT CAGAGGAATG
    GGCTCCTACC AAAACGAAGG GCACTTCAGG GACGGCAGAC CTAAGAGTCT TTTTATTCCT
    CGAGATATTC AAGATTCATT CCAGCCTGAG TTCCTAATTG CCAAGCCTGA TGATGACACA
    GATCAGGCTC ATAGCAGCTT AGAGACATCT TCTGATATTT CTCAGCCTGC TGACAGAGAA
    ACAGAGCTCG GGTTTCCTCC TCACTGGCAG CTCAATGAGT GGAAGTCCAA CAATGATCCT
    TACCGCTCAT TGTCAGGTTC AAGCACAGCC ACAGGTACCA CAGTCATTGA GTGCATGAAG
    GTGCGAGGAA GTTCAGAATC TCTGAATTCA CCCTCAACAT CAAGAGCCAC ATCCCCATCT
    CAGATGTCAA TTGAGGCTGA GACCAAGGTA TCCCCTTTTA AACCACCATG CCTCTTGTCA
    CCATCCAGTG GCTACTCAAG CCAGTCTGAA ACTCCAACCC CAACTATATC AAACACCCTT
    CTCACTGGAC CTTCTCCAGT TGGCTGCAAG ATGCGGCCAA AGATTCCAGA GCGTAAATCT
    TCCTTACCAG CCTCTGCAAA GGACAAATCA CGCTCACGTC TTTCATTTGA ACTACCTGCA
    AATGCTCAAT TGGATCTGTC CTTTGTCAAA CCCAAGCCTA AAGCCAGTAG ACGTCACTCT
    GACACATCTA CAGCCAACAA ACCAGGTAAA CTGAGCCCCA GTCAGTCTTC ACTGCCTATG
    GTAACCACAA CAGATTTGCG AAACATCCGC CTTCGTTCAG TAAGCCGCTC AGAACTTGAG
    GACAGTCCTG ACGGCTCCTC TGACATTATA GAAGAAGAAC AAAGTCGAGA TCTCAGCCCT
    CCTATCACAC CCTCTAATAC AAAACAGCCA AAGCCACCAG TAGCAATGAA GCCACCTCTC
    CCAAAGCGAC CCGTAAACTT AACACTTAAA TCTCCCTCAT CCTCCCCATT GACACCTGAA
    TCTCCTCCAT CCTCCCCTGT GGACCGACCT ATGCCTGTTG GTAATATTTA TATGGTTGTA
    AGGAAACCCA AACCTAAAAG ACCAGCTCAG GCATCACTCA GCACATCTGC AATAGCCACA
    CAAGAGCCCA TCCGCAGCCA TGTATCTACT TTTCCAGAGT TTGACCTAGA GCATGGGATT
    CCCATCGAAG AAGATCTTCC ATCCCCAAGT AGCCCAGAAA GGGAGGATAA ATGTAAAACT
    CTTCCAAGTA GAATGACAAT ATCTTGTTTG GCAGAATTAG ACAAAAGGAA GCCCAAGGTA
    CCACCACCAG TACCAAAAAA ACCCAGTGTC CTTCTACTTC CCTCTCCAAG CATCCAGACC
    AATGGTGGGG CAGAGAGGCA AACTCTACTT TCAGAAAGCA TCTCTCAGTC ACCTACCGGT
    ACATCAGCAT CTGAGATTGA GGGAGATTTG CTTAAAGATG AAGAGGAAGA CTATTCTGCC
    AAGCAAATTT CACATGAAAA CCACCAAATT CCTGAACTTT ATGAGACCTT TGGGAACGAG
    GAGAATGATG TAGTAGTTGA AGAATACAGT TCAGAAGACA GAGGTTCTCA TCATTTTATG
    GAAAGGGAGA CACCTCCAGC AGAGATAGTA AAAGAAATTT ACACAGAAAC AGAAATATTG
    GAAGAGAACA TCTCTGCAGT TGACAAAACT GAACTACACA TTACAGAGGA AACAGAGGAT
    GAAGTATTTG TCAACACACC CACAACTCAC ACCACTGAAG ATCTCTTCAC CATAATACAC
    AGGTCAAAGA GAAAAGTTCT TGGTCGAAAG GAACCATCAG ATTCTTTTGG AAGAAGTCAG
    TGTCTGGTCT CGCCTGTGAA AAGCAGTAGT ACAGACTTCA GAACTCTGGG CCTTGGGACT
    ACACCTCGGT CAAGCTCACG AAATGAAAAC TTTATGGCTC TCCTCCAAAA GAGGAGCAGC
    AAAGCTAGCA GTGGGACCAG AGTTTCTGCC ATGGAGTTGC TGAAGAGCAC AAACCCTTTG
    GCACGACGAG TCACGGAATT CTCTCAGTCA CAACCAGATG GGAGCGCAAT GAACACACCA
    AAACCATCTT CGCAGGACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333632.1
    CDS610..3393
    Translation

    Target ORF information:

    RefSeq Version XM_021477957.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021477957.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGGATC ATGCCACTCT AATGTGCCCA AGCCCTTCCT GGAATGGCCC AAAAGGTTCC 
    ACTTTCTCTC CCTCCTGGAA TGACTCCTAC AACTATGCCT TGGTTACCAG CCCTGCTTCC
    AAGCAGCCAG TGACTAAAAC TGGAATGTTA ACACCTGGAG GTGATAGGGG TGGTCTAATT
    TGTCCATCTC AGACTAGTTC AGGCCTCTCC ATGAGTTCAG GGTCACACTC CAGTTCTTTC
    ACCTCCATCT CTGAACCATG TGGGCACAGA GTATACACAG TGTCCACACA GGGGAGTGGA
    GTTGTAACAG GGAAACGGAA TGAGACTGAG GTGACAGCTT CTACTGCAGT CACAAGAAAT
    AGCAAAAGTG AGCAGGAAAA GAGATCAGCC CGATCCAATG TCTTCAGGTT CCGTGAGCGC
    TCACTTTCAA CACCAACAGA CTCTGGATCA TTTTGCTCAG CAGATAATAT ATGTTCTCCA
    GGCGAGGCTG TCTCTGGGGT TCCAGAGGGT GAAAGTTATG CCTTGCTTTA TCCGAGTAAC
    AGCTCGGAGG ATAGCACGAG CACTGATAAT GTTTCTGTGA CCACAGGTTC AGACTTTCTT
    CCTGATGGTC GACTAAGGTC ACGGTCACGC AGTATCTCAC TAAAGAAATC TAAAAAAAAG
    CCACCACCTC CTGTGCGTAG TGTATCTCTG ATGAAAAACC TATCAGAAAT CAGAGGAATG
    GGCTCCTACC AAAACGAAGG GCACTTCAGG GACGGCAGAC CTAAGAGTCT TTTTATTCCT
    CGAGATATTC AAGATTCATT CCAGCCTGAG TTCCTAATTG CCAAGCCTGA TGATGACACA
    GATCAGGCTC ATAGCAGCTT AGAGACATCT TCTGATATTT CTCAGCCTGC TGACAGAGAA
    ACAGAGCTCG GGTTTCCTCC TCACTGGCAG CTCAATGAGT GGAAGTCCAA CAATGATCCT
    TACCGCTCAT TGTCAGGTTC AAGCACAGCC ACAGGTACCA CAGTCATTGA GTGCATGAAG
    GTGCGAGGAA GTTCAGAATC TCTGAATTCA CCCTCAACAT CAAGAGCCAC ATCCCCATCT
    CAGATGTCAA TTGAGGCTGA GACCAAGGTA TCCCCTTTTA AACCACCATG CCTCTTGTCA
    CCATCCAGTG GCTACTCAAG CCAGTCTGAA ACTCCAACCC CAACTATATC AAACACCCTT
    CTCACTGGAC CTTCTCCAGT TGGCTGCAAG ATGCGGCCAA AGATTCCAGA GCGTAAATCT
    TCCTTACCAG CCTCTGCAAA GGACAAATCA CGCTCACGTC TTTCATTTGA ACTACCTGCA
    AATGCTCAAT TGGATCTGTC CTTTGTCAAA CCCAAGCCTA AAGCCAGTAG ACGTCACTCT
    GACACATCTA CAGCCAACAA ACCAGGTAAA CTGAGCCCCA GTCAGTCTTC ACTGCCTATG
    GTAACCACAA CAGATTTGCG AAACATCCGC CTTCGTTCAG TAAGCCGCTC AGAACTTGAG
    GACAGTCCTG ACGGCTCCTC TGACATTATA GAAGAAGAAC AAAGTCGAGA TCTCAGCCCT
    CCTATCACAC CCTCTAATAC AAAACAGCCA AAGCCACCAG TAGCAATGAA GCCACCTCTC
    CCAAAGCGAC CCGTAAACTT AACACTTAAA TCTCCCTCAT CCTCCCCATT GACACCTGAA
    TCTCCTCCAT CCTCCCCTGT GGACCGACCT ATGCCTGTTG GTAATATTTA TATGGTTGTA
    AGGAAACCCA AACCTAAAAG ACCAGCTCAG GCATCACTCA GCACATCTGC AATAGCCACA
    CAAGAGCCCA TCCGCAGCCA TGTATCTACT TTTCCAGAGT TTGACCTAGA GCATGGGATT
    CCCATCGAAG AAGATCTTCC ATCCCCAAGT AGCCCAGAAA GGGAGGATAA ATGTAAAACT
    CTTCCAAGTA GAATGACAAT ATCTTGTTTG GCAGAATTAG ACAAAAGGAA GCCCAAGGTA
    CCACCACCAG TACCAAAAAA ACCCAGTGTC CTTCTACTTC CCTCTCCAAG CATCCAGACC
    AATGGTGGGG CAGAGAGGCA AACTCTACTT TCAGAAAGCA TCTCTCAGTC ACCTACCGGT
    ACATCAGCAT CTGAGATTGA GGGAGATTTG CTTAAAGATG AAGAGGAAGA CTATTCTGCC
    AAGCAAATTT CACATGAAAA CCACCAAATT CCTGAACTTT ATGAGACCTT TGGGAACGAG
    GAGAATGATG TAGTAGTTGA AGAATACAGT TCAGAAGACA GAGGTTCTCA TCATTTTATG
    GAAAGGGAGA CACCTCCAGC AGAGATAGTA AAAGAAATTT ACACAGAAAC AGAAATATTG
    GAAGAGAACA TCTCTGCAGT TGACAAAACT GAACTACACA TTACAGAGGA AACAGAGGAT
    GAAGTATTTG TCAACACACC CACAACTCAC ACCACTGAAG ATCTCTTCAC CATAATACAC
    AGGTCAAAGA GAAAAGTTCT TGGTCGAAAG GAACCATCAG ATTCTTTTGG AAGAAGTCAG
    TGTCTGGTCT CGCCTGTGAA AAGCAGTAGT ACAGACTTCA GAACTCTGGG CCTTGGGACT
    ACACCTCGGT CAAGCTCACG AAATGAAAAC TTTATGGCTC TCCTCCAAAA GAGGAGCAGC
    AAAGCTAGCA GTGGGACCAG AGTTTCTGCC ATGGAGTTGC TGAAGAGCAC AAACCCTTTG
    GCACGACGAG TCACGGAATT CTCTCAGTCA CAACCAGATG GGAGCGCAAT GAACACACCA
    AAACCATCTT CGCAGGACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46887
    Clone ID Related Accession (Same CDS sequence) XM_021477957.1 , XM_009303453.2
    Accession Version XM_009303453.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2784bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303453.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGGATC ATGCCACTCT AATGTGCCCA AGCCCTTCCT GGAATGGCCC AAAAGGTTCC 
    ACTTTCTCTC CCTCCTGGAA TGACTCCTAC AACTATGCCT TGGTTACCAG CCCTGCTTCC
    AAGCAGCCAG TGACTAAAAC TGGAATGTTA ACACCTGGAG GTGATAGGGG TGGTCTAATT
    TGTCCATCTC AGACTAGTTC AGGCCTCTCC ATGAGTTCAG GGTCACACTC CAGTTCTTTC
    ACCTCCATCT CTGAACCATG TGGGCACAGA GTATACACAG TGTCCACACA GGGGAGTGGA
    GTTGTAACAG GGAAACGGAA TGAGACTGAG GTGACAGCTT CTACTGCAGT CACAAGAAAT
    AGCAAAAGTG AGCAGGAAAA GAGATCAGCC CGATCCAATG TCTTCAGGTT CCGTGAGCGC
    TCACTTTCAA CACCAACAGA CTCTGGATCA TTTTGCTCAG CAGATAATAT ATGTTCTCCA
    GGCGAGGCTG TCTCTGGGGT TCCAGAGGGT GAAAGTTATG CCTTGCTTTA TCCGAGTAAC
    AGCTCGGAGG ATAGCACGAG CACTGATAAT GTTTCTGTGA CCACAGGTTC AGACTTTCTT
    CCTGATGGTC GACTAAGGTC ACGGTCACGC AGTATCTCAC TAAAGAAATC TAAAAAAAAG
    CCACCACCTC CTGTGCGTAG TGTATCTCTG ATGAAAAACC TATCAGAAAT CAGAGGAATG
    GGCTCCTACC AAAACGAAGG GCACTTCAGG GACGGCAGAC CTAAGAGTCT TTTTATTCCT
    CGAGATATTC AAGATTCATT CCAGCCTGAG TTCCTAATTG CCAAGCCTGA TGATGACACA
    GATCAGGCTC ATAGCAGCTT AGAGACATCT TCTGATATTT CTCAGCCTGC TGACAGAGAA
    ACAGAGCTCG GGTTTCCTCC TCACTGGCAG CTCAATGAGT GGAAGTCCAA CAATGATCCT
    TACCGCTCAT TGTCAGGTTC AAGCACAGCC ACAGGTACCA CAGTCATTGA GTGCATGAAG
    GTGCGAGGAA GTTCAGAATC TCTGAATTCA CCCTCAACAT CAAGAGCCAC ATCCCCATCT
    CAGATGTCAA TTGAGGCTGA GACCAAGGTA TCCCCTTTTA AACCACCATG CCTCTTGTCA
    CCATCCAGTG GCTACTCAAG CCAGTCTGAA ACTCCAACCC CAACTATATC AAACACCCTT
    CTCACTGGAC CTTCTCCAGT TGGCTGCAAG ATGCGGCCAA AGATTCCAGA GCGTAAATCT
    TCCTTACCAG CCTCTGCAAA GGACAAATCA CGCTCACGTC TTTCATTTGA ACTACCTGCA
    AATGCTCAAT TGGATCTGTC CTTTGTCAAA CCCAAGCCTA AAGCCAGTAG ACGTCACTCT
    GACACATCTA CAGCCAACAA ACCAGGTAAA CTGAGCCCCA GTCAGTCTTC ACTGCCTATG
    GTAACCACAA CAGATTTGCG AAACATCCGC CTTCGTTCAG TAAGCCGCTC AGAACTTGAG
    GACAGTCCTG ACGGCTCCTC TGACATTATA GAAGAAGAAC AAAGTCGAGA TCTCAGCCCT
    CCTATCACAC CCTCTAATAC AAAACAGCCA AAGCCACCAG TAGCAATGAA GCCACCTCTC
    CCAAAGCGAC CCGTAAACTT AACACTTAAA TCTCCCTCAT CCTCCCCATT GACACCTGAA
    TCTCCTCCAT CCTCCCCTGT GGACCGACCT ATGCCTGTTG GTAATATTTA TATGGTTGTA
    AGGAAACCCA AACCTAAAAG ACCAGCTCAG GCATCACTCA GCACATCTGC AATAGCCACA
    CAAGAGCCCA TCCGCAGCCA TGTATCTACT TTTCCAGAGT TTGACCTAGA GCATGGGATT
    CCCATCGAAG AAGATCTTCC ATCCCCAAGT AGCCCAGAAA GGGAGGATAA ATGTAAAACT
    CTTCCAAGTA GAATGACAAT ATCTTGTTTG GCAGAATTAG ACAAAAGGAA GCCCAAGGTA
    CCACCACCAG TACCAAAAAA ACCCAGTGTC CTTCTACTTC CCTCTCCAAG CATCCAGACC
    AATGGTGGGG CAGAGAGGCA AACTCTACTT TCAGAAAGCA TCTCTCAGTC ACCTACCGGT
    ACATCAGCAT CTGAGATTGA GGGAGATTTG CTTAAAGATG AAGAGGAAGA CTATTCTGCC
    AAGCAAATTT CACATGAAAA CCACCAAATT CCTGAACTTT ATGAGACCTT TGGGAACGAG
    GAGAATGATG TAGTAGTTGA AGAATACAGT TCAGAAGACA GAGGTTCTCA TCATTTTATG
    GAAAGGGAGA CACCTCCAGC AGAGATAGTA AAAGAAATTT ACACAGAAAC AGAAATATTG
    GAAGAGAACA TCTCTGCAGT TGACAAAACT GAACTACACA TTACAGAGGA AACAGAGGAT
    GAAGTATTTG TCAACACACC CACAACTCAC ACCACTGAAG ATCTCTTCAC CATAATACAC
    AGGTCAAAGA GAAAAGTTCT TGGTCGAAAG GAACCATCAG ATTCTTTTGG AAGAAGTCAG
    TGTCTGGTCT CGCCTGTGAA AAGCAGTAGT ACAGACTTCA GAACTCTGGG CCTTGGGACT
    ACACCTCGGT CAAGCTCACG AAATGAAAAC TTTATGGCTC TCCTCCAAAA GAGGAGCAGC
    AAAGCTAGCA GTGGGACCAG AGTTTCTGCC ATGGAGTTGC TGAAGAGCAC AAACCCTTTG
    GCACGACGAG TCACGGAATT CTCTCAGTCA CAACCAGATG GGAGCGCAAT GAACACACCA
    AAACCATCTT CGCAGGACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301728.1
    CDS569..3352
    Translation

    Target ORF information:

    RefSeq Version XM_009303453.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303453.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGATGGATC ATGCCACTCT AATGTGCCCA AGCCCTTCCT GGAATGGCCC AAAAGGTTCC 
    ACTTTCTCTC CCTCCTGGAA TGACTCCTAC AACTATGCCT TGGTTACCAG CCCTGCTTCC
    AAGCAGCCAG TGACTAAAAC TGGAATGTTA ACACCTGGAG GTGATAGGGG TGGTCTAATT
    TGTCCATCTC AGACTAGTTC AGGCCTCTCC ATGAGTTCAG GGTCACACTC CAGTTCTTTC
    ACCTCCATCT CTGAACCATG TGGGCACAGA GTATACACAG TGTCCACACA GGGGAGTGGA
    GTTGTAACAG GGAAACGGAA TGAGACTGAG GTGACAGCTT CTACTGCAGT CACAAGAAAT
    AGCAAAAGTG AGCAGGAAAA GAGATCAGCC CGATCCAATG TCTTCAGGTT CCGTGAGCGC
    TCACTTTCAA CACCAACAGA CTCTGGATCA TTTTGCTCAG CAGATAATAT ATGTTCTCCA
    GGCGAGGCTG TCTCTGGGGT TCCAGAGGGT GAAAGTTATG CCTTGCTTTA TCCGAGTAAC
    AGCTCGGAGG ATAGCACGAG CACTGATAAT GTTTCTGTGA CCACAGGTTC AGACTTTCTT
    CCTGATGGTC GACTAAGGTC ACGGTCACGC AGTATCTCAC TAAAGAAATC TAAAAAAAAG
    CCACCACCTC CTGTGCGTAG TGTATCTCTG ATGAAAAACC TATCAGAAAT CAGAGGAATG
    GGCTCCTACC AAAACGAAGG GCACTTCAGG GACGGCAGAC CTAAGAGTCT TTTTATTCCT
    CGAGATATTC AAGATTCATT CCAGCCTGAG TTCCTAATTG CCAAGCCTGA TGATGACACA
    GATCAGGCTC ATAGCAGCTT AGAGACATCT TCTGATATTT CTCAGCCTGC TGACAGAGAA
    ACAGAGCTCG GGTTTCCTCC TCACTGGCAG CTCAATGAGT GGAAGTCCAA CAATGATCCT
    TACCGCTCAT TGTCAGGTTC AAGCACAGCC ACAGGTACCA CAGTCATTGA GTGCATGAAG
    GTGCGAGGAA GTTCAGAATC TCTGAATTCA CCCTCAACAT CAAGAGCCAC ATCCCCATCT
    CAGATGTCAA TTGAGGCTGA GACCAAGGTA TCCCCTTTTA AACCACCATG CCTCTTGTCA
    CCATCCAGTG GCTACTCAAG CCAGTCTGAA ACTCCAACCC CAACTATATC AAACACCCTT
    CTCACTGGAC CTTCTCCAGT TGGCTGCAAG ATGCGGCCAA AGATTCCAGA GCGTAAATCT
    TCCTTACCAG CCTCTGCAAA GGACAAATCA CGCTCACGTC TTTCATTTGA ACTACCTGCA
    AATGCTCAAT TGGATCTGTC CTTTGTCAAA CCCAAGCCTA AAGCCAGTAG ACGTCACTCT
    GACACATCTA CAGCCAACAA ACCAGGTAAA CTGAGCCCCA GTCAGTCTTC ACTGCCTATG
    GTAACCACAA CAGATTTGCG AAACATCCGC CTTCGTTCAG TAAGCCGCTC AGAACTTGAG
    GACAGTCCTG ACGGCTCCTC TGACATTATA GAAGAAGAAC AAAGTCGAGA TCTCAGCCCT
    CCTATCACAC CCTCTAATAC AAAACAGCCA AAGCCACCAG TAGCAATGAA GCCACCTCTC
    CCAAAGCGAC CCGTAAACTT AACACTTAAA TCTCCCTCAT CCTCCCCATT GACACCTGAA
    TCTCCTCCAT CCTCCCCTGT GGACCGACCT ATGCCTGTTG GTAATATTTA TATGGTTGTA
    AGGAAACCCA AACCTAAAAG ACCAGCTCAG GCATCACTCA GCACATCTGC AATAGCCACA
    CAAGAGCCCA TCCGCAGCCA TGTATCTACT TTTCCAGAGT TTGACCTAGA GCATGGGATT
    CCCATCGAAG AAGATCTTCC ATCCCCAAGT AGCCCAGAAA GGGAGGATAA ATGTAAAACT
    CTTCCAAGTA GAATGACAAT ATCTTGTTTG GCAGAATTAG ACAAAAGGAA GCCCAAGGTA
    CCACCACCAG TACCAAAAAA ACCCAGTGTC CTTCTACTTC CCTCTCCAAG CATCCAGACC
    AATGGTGGGG CAGAGAGGCA AACTCTACTT TCAGAAAGCA TCTCTCAGTC ACCTACCGGT
    ACATCAGCAT CTGAGATTGA GGGAGATTTG CTTAAAGATG AAGAGGAAGA CTATTCTGCC
    AAGCAAATTT CACATGAAAA CCACCAAATT CCTGAACTTT ATGAGACCTT TGGGAACGAG
    GAGAATGATG TAGTAGTTGA AGAATACAGT TCAGAAGACA GAGGTTCTCA TCATTTTATG
    GAAAGGGAGA CACCTCCAGC AGAGATAGTA AAAGAAATTT ACACAGAAAC AGAAATATTG
    GAAGAGAACA TCTCTGCAGT TGACAAAACT GAACTACACA TTACAGAGGA AACAGAGGAT
    GAAGTATTTG TCAACACACC CACAACTCAC ACCACTGAAG ATCTCTTCAC CATAATACAC
    AGGTCAAAGA GAAAAGTTCT TGGTCGAAAG GAACCATCAG ATTCTTTTGG AAGAAGTCAG
    TGTCTGGTCT CGCCTGTGAA AAGCAGTAGT ACAGACTTCA GAACTCTGGG CCTTGGGACT
    ACACCTCGGT CAAGCTCACG AAATGAAAAC TTTATGGCTC TCCTCCAAAA GAGGAGCAGC
    AAAGCTAGCA GTGGGACCAG AGTTTCTGCC ATGGAGTTGC TGAAGAGCAC AAACCCTTTG
    GCACGACGAG TCACGGAATT CTCTCAGTCA CAACCAGATG GGAGCGCAAT GAACACACCA
    AAACCATCTT CGCAGGACCA GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13435
    Clone ID Related Accession (Same CDS sequence) XM_001334017.7 , XM_001334017.6
    Accession Version XM_001334017.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001334017.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTTTGGG ACAGGAAACA CGGAGCTATG GGCAATTCCC AACGCCGGGC TAAAGGGAGG 
    CATAAAACCA AGGGGGCAAC AGCCGCCACC AACTTGGACT CGGAGAGCAA ACGAACGGCC
    CACTTCCAAT CGTCATGGCA ACAGCATGTG AATGTGTTCG GCTCGTGGAG TAGGCCGGAG
    TGTGTGCAGG AGCTGCACCA GGAGGCTCAG CTCAACCTAC AGAGTCTGCT GCAAGATTTC
    GAGGAGCAGT TGTATGATAA CAGAGTGACA GGACAGACAT TCAAACATCC TTCCTCTCAG
    AGCTCGGAGG ACACGTCAAC AACTCTCAGC CGGTCGCCTT CCTCACTCAA CAACAAGAAG
    ACGGAATTCG TCTTTGTGCC TGCAAATAAG CAGGTGTGTG AGGATGAGAC CACAACTTTA
    GGGGTCCGGG CGCAGGACCC GAGCCCTTGC GCTTCAGAGA AGACTCTTTT AGGCTGGAAT
    GCTGCACTGA GTCCACCTGT TGCAGAGAAA CCTCGCTGGT ACCTTGGCCG ACAAACTCCA
    GCTCAGCTTG TACCCATTGA GATCACAGGC CAGAGCTTTG ATAAGCACGC CGCACGGCAC
    AGCGAACTGC GGGCCACCAT TAGAACTGAT TCAGCGGTCA ACCCAAAAAC CATTCGCCGC
    CCGAGGAGTG TGGTTGCAGG CCCTGATGTC ACGCTGCACT GCCAAGGCGA CAGCAAAATT
    CTTGCTGTTG ATTTGATACA AGGTGCCTGT TCCCCTGGAT CATCTCAACC TAGATCTTTG
    GAGCCTACCA CAGAGAATAC AGGTCAACAG CATATTCCTC TTCGAAAGAC TCAGAGTGAC
    CTGGAGTACA GCATACCCCC ATCCCCAAAG ACCCCATCAG GCATGATGGA TCATGCCACT
    CTAATGTGCC CAAGCCCTTC CTGGAATGGC CCAAAAGGTT CCACTTTCTC TCCCTCCTGG
    AATGACTCCT ACAACTATGC CTTGGTTACC AGCCCTGCTT CCAAGCAGCC AGTGACTAAA
    ACTGGAATGT TAACACCTGG AGGTGATAGG GGTGGTCTAA TTTGTCCATC TCAGACTAGT
    TCAGGCCTCT CCATGAGTTC AGGGTCACAC TCCAGTTCTT TCACCTCCAT CTCTGAACCA
    TGTGGGCACA GAGTATACAC AGTGTCCACA CAGGGGAGTG GAGTTGTAAC AGGGAAACGG
    AATGAGACTG AGGTGACAGC TTCTACTGCA GTCACAAGAA ATAGCAAAAG TGAGCAGGAA
    AAGAGATCAG CCCGATCCAA TGTCTTCAGG TTCCGTGAGC GCTCACTTTC AACACCAACA
    GACTCTGGAT CATTTTGCTC AGCAGATAAT ATATGTTCTC CAGGCGAGGC TGTCTCTGGG
    GTTCCAGAGG GTGAAAGTTA TGCCTTGCTT TATCCGAGTA ACAGCTCGGA GGATAGCACG
    AGCACTGATA ATGTTTCTGT GACCACAGGT TCAGACTTTC TTCCTGATGG TCGACTAAGG
    TCACGGTCAC GCAGTATCTC ACTAAAGAAA TCTAAAAAAA AGCCACCACC TCCTGTGCGT
    AGTGTATCTC TGATGAAAAA CCTATCAGAA ATCAGAGGAA TGGGCTCCTA CCAAAACGAA
    GGGCACTTCA GGGACGGCAG ACCTAAGAGT CTTTTTATTC CTCGAGATAT TCAAGATTCA
    TTCCAGCCTG AGTTCCTAAT TGCCAAGCCT GATGATGACA CAGATCAGGC TCATAGCAGC
    TTAGAGACAT CTTCTGATAT TTCTCAGCCT GCTGACAGAG AAACAGAGCT CGGGTTTCCT
    CCTCACTGGC AGCTCAATGA GTGGAAGTCC AACAATGATC CTTACCGCTC ATTGTCAGGT
    TCAAGCACAG CCACAGGTAC CACAGTCATT GAGTGCATGA AGGTGCGAGG AAGTTCAGAA
    TCTCTGAATT CACCCTCAAC ATCAAGAGCC ACATCCCCAT CTCAGATGTC AATTGAGGCT
    GAGACCAAGG TATCCCCTTT TAAACCACCA TGCCTCTTGT CACCATCCAG TGGCTACTCA
    AGCCAGTCTG AAACTCCAAC CCCAACTATA TCAAACACCC TTCTCACTGG ACCTTCTCCA
    GTTGGCTGCA AGATGCGGCC AAAGATTCCA GAGCGTAAAT CTTCCTTACC AGCCTCTGCA
    AAGGACAAAT CACGCTCACG TCTTTCATTT GAACTACCTG CAAATGCTCA ATTGGATCTG
    TCCTTTGTCA AACCCAAGCC TAAAGCCAGT AGACGTCACT CTGACACATC TACAGCCAAC
    AAACCAGGTA AACTGAGCCC CAGTCAGTCT TCACTGCCTA TGGTAACCAC AACAGATTTG
    CGAAACATCC GCCTTCGTTC AGTAAGCCGC TCAGAACTTG AGGACAGTCC TGACGGCTCC
    TCTGACATTA TAGAAGAAGA ACAAAGTCGA GATCTCAGCC CTCCTATCAC ACCCTCTAAT
    ACAAAACAGC CAAAGCCACC AGTAGCAATG AAGCCACCTC TCCCAAAGCG ACCCGTAAAC
    TTAACACTTA AATCTCCCTC ATCCTCCCCA TTGACACCTG AATCTCCTCC ATCCTCCCCT
    GTGGACCGAC CTATGCCTGT TGGTAATATT TATATGGTTG TAAGGAAACC CAAACCTAAA
    AGACCAGCTC AGGCATCACT CAGCACATCT GCAATAGCCA CACAAGAGCC CATCCGCAGC
    CATGTATCTA CTTTTCCAGA GTTTGACCTA GAGCATGGGA TTCCCATCGA AGAAGATCTT
    CCATCCCCAA GTAGCCCAGA AAGGGAGGAT AAATGTAAAA CTCTTCCAAG TAGAATGACA
    ATATCTTGTT TGGCAGAATT AGACAAAAGG AAGCCCAAGG TACCACCACC AGTACCAAAA
    AAACCCAGTG TCCTTCTACT TCCCTCTCCA AGCATCCAGA CCAATGGTGG GGCAGAGAGG
    CAAACTCTAC TTTCAGAAAG CATCTCTCAG TCACCTACCG GTACATCAGC ATCTGAGATT
    GAGGGAGATT TGCTTAAAGA TGAAGAGGAA GACTATTCTG CCAAGCAAAT TTCACATGAA
    AACCACCAAA TTCCTGAACT TTATGAGACC TTTGGGAACG AGGAGAATGA TGTAGTAGTT
    GAAGAATACA GTTCAGAAGA CAGAGGTTCT CATCATTTTA TGGAAAGGGA GACACCTCCA
    GCAGAGATAG TAAAAGAAAT TTACACAGAA ACAGAAATAT TGGAAGAGAA CATCTCTGCA
    GTTGACAAAA CTGAACTACA CATTACAGAG GAAACAGAGG ATGAAGTATT TGTCAACACA
    CCCACAACTC ACACCACTGA AGATCTCTTC ACCATAATAC ACAGGTCAAA GAGAAAAGTT
    CTTGGTCGAA AGGAACCATC AGATTCTTTT GGAAGAAGTC AGTGTCTGGT CTCGCCTGTG
    AAAAGCAGTA GTACAGACTT CAGAACTCTG GGCCTTGGGA CTACACCTCG GTCAAGCTCA
    CGAAATGAAA ACTTTATGGC TCTCCTCCAA AAGAGGAGCA GCAAAGCTAG CAGTGGGACC
    AGAGTTTCTG CCATGGAGTT GCTGAAGAGC ACAAACCCTT TGGCACGACG AGTCACGGAA
    TTCTCTCAGT CACAACCAGA TGGGAGCGCA ATGAACACAC CAAAACCATC TTCGCAGGAC
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001334053.3
    CDS288..3953
    Translation

    Target ORF information:

    RefSeq Version XM_001334017.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001334017.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTTTGGG ACAGGAAACA CGGAGCTATG GGCAATTCCC AACGCCGGGC TAAAGGGAGG 
    CATAAAACCA AGGGGGCAAC AGCCGCCACC AACTTGGACT CGGAGAGCAA ACGAACGGCC
    CACTTCCAAT CGTCATGGCA ACAGCATGTG AATGTGTTCG GCTCGTGGAG TAGGCCGGAG
    TGTGTGCAGG AGCTGCACCA GGAGGCTCAG CTCAACCTAC AGAGTCTGCT GCAAGATTTC
    GAGGAGCAGT TGTATGATAA CAGAGTGACA GGACAGACAT TCAAACATCC TTCCTCTCAG
    AGCTCGGAGG ACACGTCAAC AACTCTCAGC CGGTCGCCTT CCTCACTCAA CAACAAGAAG
    ACGGAATTCG TCTTTGTGCC TGCAAATAAG CAGGTGTGTG AGGATGAGAC CACAACTTTA
    GGGGTCCGGG CGCAGGACCC GAGCCCTTGC GCTTCAGAGA AGACTCTTTT AGGCTGGAAT
    GCTGCACTGA GTCCACCTGT TGCAGAGAAA CCTCGCTGGT ACCTTGGCCG ACAAACTCCA
    GCTCAGCTTG TACCCATTGA GATCACAGGC CAGAGCTTTG ATAAGCACGC CGCACGGCAC
    AGCGAACTGC GGGCCACCAT TAGAACTGAT TCAGCGGTCA ACCCAAAAAC CATTCGCCGC
    CCGAGGAGTG TGGTTGCAGG CCCTGATGTC ACGCTGCACT GCCAAGGCGA CAGCAAAATT
    CTTGCTGTTG ATTTGATACA AGGTGCCTGT TCCCCTGGAT CATCTCAACC TAGATCTTTG
    GAGCCTACCA CAGAGAATAC AGGTCAACAG CATATTCCTC TTCGAAAGAC TCAGAGTGAC
    CTGGAGTACA GCATACCCCC ATCCCCAAAG ACCCCATCAG GCATGATGGA TCATGCCACT
    CTAATGTGCC CAAGCCCTTC CTGGAATGGC CCAAAAGGTT CCACTTTCTC TCCCTCCTGG
    AATGACTCCT ACAACTATGC CTTGGTTACC AGCCCTGCTT CCAAGCAGCC AGTGACTAAA
    ACTGGAATGT TAACACCTGG AGGTGATAGG GGTGGTCTAA TTTGTCCATC TCAGACTAGT
    TCAGGCCTCT CCATGAGTTC AGGGTCACAC TCCAGTTCTT TCACCTCCAT CTCTGAACCA
    TGTGGGCACA GAGTATACAC AGTGTCCACA CAGGGGAGTG GAGTTGTAAC AGGGAAACGG
    AATGAGACTG AGGTGACAGC TTCTACTGCA GTCACAAGAA ATAGCAAAAG TGAGCAGGAA
    AAGAGATCAG CCCGATCCAA TGTCTTCAGG TTCCGTGAGC GCTCACTTTC AACACCAACA
    GACTCTGGAT CATTTTGCTC AGCAGATAAT ATATGTTCTC CAGGCGAGGC TGTCTCTGGG
    GTTCCAGAGG GTGAAAGTTA TGCCTTGCTT TATCCGAGTA ACAGCTCGGA GGATAGCACG
    AGCACTGATA ATGTTTCTGT GACCACAGGT TCAGACTTTC TTCCTGATGG TCGACTAAGG
    TCACGGTCAC GCAGTATCTC ACTAAAGAAA TCTAAAAAAA AGCCACCACC TCCTGTGCGT
    AGTGTATCTC TGATGAAAAA CCTATCAGAA ATCAGAGGAA TGGGCTCCTA CCAAAACGAA
    GGGCACTTCA GGGACGGCAG ACCTAAGAGT CTTTTTATTC CTCGAGATAT TCAAGATTCA
    TTCCAGCCTG AGTTCCTAAT TGCCAAGCCT GATGATGACA CAGATCAGGC TCATAGCAGC
    TTAGAGACAT CTTCTGATAT TTCTCAGCCT GCTGACAGAG AAACAGAGCT CGGGTTTCCT
    CCTCACTGGC AGCTCAATGA GTGGAAGTCC AACAATGATC CTTACCGCTC ATTGTCAGGT
    TCAAGCACAG CCACAGGTAC CACAGTCATT GAGTGCATGA AGGTGCGAGG AAGTTCAGAA
    TCTCTGAATT CACCCTCAAC ATCAAGAGCC ACATCCCCAT CTCAGATGTC AATTGAGGCT
    GAGACCAAGG TATCCCCTTT TAAACCACCA TGCCTCTTGT CACCATCCAG TGGCTACTCA
    AGCCAGTCTG AAACTCCAAC CCCAACTATA TCAAACACCC TTCTCACTGG ACCTTCTCCA
    GTTGGCTGCA AGATGCGGCC AAAGATTCCA GAGCGTAAAT CTTCCTTACC AGCCTCTGCA
    AAGGACAAAT CACGCTCACG TCTTTCATTT GAACTACCTG CAAATGCTCA ATTGGATCTG
    TCCTTTGTCA AACCCAAGCC TAAAGCCAGT AGACGTCACT CTGACACATC TACAGCCAAC
    AAACCAGGTA AACTGAGCCC CAGTCAGTCT TCACTGCCTA TGGTAACCAC AACAGATTTG
    CGAAACATCC GCCTTCGTTC AGTAAGCCGC TCAGAACTTG AGGACAGTCC TGACGGCTCC
    TCTGACATTA TAGAAGAAGA ACAAAGTCGA GATCTCAGCC CTCCTATCAC ACCCTCTAAT
    ACAAAACAGC CAAAGCCACC AGTAGCAATG AAGCCACCTC TCCCAAAGCG ACCCGTAAAC
    TTAACACTTA AATCTCCCTC ATCCTCCCCA TTGACACCTG AATCTCCTCC ATCCTCCCCT
    GTGGACCGAC CTATGCCTGT TGGTAATATT TATATGGTTG TAAGGAAACC CAAACCTAAA
    AGACCAGCTC AGGCATCACT CAGCACATCT GCAATAGCCA CACAAGAGCC CATCCGCAGC
    CATGTATCTA CTTTTCCAGA GTTTGACCTA GAGCATGGGA TTCCCATCGA AGAAGATCTT
    CCATCCCCAA GTAGCCCAGA AAGGGAGGAT AAATGTAAAA CTCTTCCAAG TAGAATGACA
    ATATCTTGTT TGGCAGAATT AGACAAAAGG AAGCCCAAGG TACCACCACC AGTACCAAAA
    AAACCCAGTG TCCTTCTACT TCCCTCTCCA AGCATCCAGA CCAATGGTGG GGCAGAGAGG
    CAAACTCTAC TTTCAGAAAG CATCTCTCAG TCACCTACCG GTACATCAGC ATCTGAGATT
    GAGGGAGATT TGCTTAAAGA TGAAGAGGAA GACTATTCTG CCAAGCAAAT TTCACATGAA
    AACCACCAAA TTCCTGAACT TTATGAGACC TTTGGGAACG AGGAGAATGA TGTAGTAGTT
    GAAGAATACA GTTCAGAAGA CAGAGGTTCT CATCATTTTA TGGAAAGGGA GACACCTCCA
    GCAGAGATAG TAAAAGAAAT TTACACAGAA ACAGAAATAT TGGAAGAGAA CATCTCTGCA
    GTTGACAAAA CTGAACTACA CATTACAGAG GAAACAGAGG ATGAAGTATT TGTCAACACA
    CCCACAACTC ACACCACTGA AGATCTCTTC ACCATAATAC ACAGGTCAAA GAGAAAAGTT
    CTTGGTCGAA AGGAACCATC AGATTCTTTT GGAAGAAGTC AGTGTCTGGT CTCGCCTGTG
    AAAAGCAGTA GTACAGACTT CAGAACTCTG GGCCTTGGGA CTACACCTCG GTCAAGCTCA
    CGAAATGAAA ACTTTATGGC TCTCCTCCAA AAGAGGAGCA GCAAAGCTAG CAGTGGGACC
    AGAGTTTCTG CCATGGAGTT GCTGAAGAGC ACAAACCCTT TGGCACGACG AGTCACGGAA
    TTCTCTCAGT CACAACCAGA TGGGAGCGCA ATGAACACAC CAAAACCATC TTCGCAGGAC
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13435
    Clone ID Related Accession (Same CDS sequence) XM_001334017.7 , XM_001334017.6
    Accession Version XM_001334017.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3666bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001334017.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTTTGGG ACAGGAAACA CGGAGCTATG GGCAATTCCC AACGCCGGGC TAAAGGGAGG 
    CATAAAACCA AGGGGGCAAC AGCCGCCACC AACTTGGACT CGGAGAGCAA ACGAACGGCC
    CACTTCCAAT CGTCATGGCA ACAGCATGTG AATGTGTTCG GCTCGTGGAG TAGGCCGGAG
    TGTGTGCAGG AGCTGCACCA GGAGGCTCAG CTCAACCTAC AGAGTCTGCT GCAAGATTTC
    GAGGAGCAGT TGTATGATAA CAGAGTGACA GGACAGACAT TCAAACATCC TTCCTCTCAG
    AGCTCGGAGG ACACGTCAAC AACTCTCAGC CGGTCGCCTT CCTCACTCAA CAACAAGAAG
    ACGGAATTCG TCTTTGTGCC TGCAAATAAG CAGGTGTGTG AGGATGAGAC CACAACTTTA
    GGGGTCCGGG CGCAGGACCC GAGCCCTTGC GCTTCAGAGA AGACTCTTTT AGGCTGGAAT
    GCTGCACTGA GTCCACCTGT TGCAGAGAAA CCTCGCTGGT ACCTTGGCCG ACAAACTCCA
    GCTCAGCTTG TACCCATTGA GATCACAGGC CAGAGCTTTG ATAAGCACGC CGCACGGCAC
    AGCGAACTGC GGGCCACCAT TAGAACTGAT TCAGCGGTCA ACCCAAAAAC CATTCGCCGC
    CCGAGGAGTG TGGTTGCAGG CCCTGATGTC ACGCTGCACT GCCAAGGCGA CAGCAAAATT
    CTTGCTGTTG ATTTGATACA AGGTGCCTGT TCCCCTGGAT CATCTCAACC TAGATCTTTG
    GAGCCTACCA CAGAGAATAC AGGTCAACAG CATATTCCTC TTCGAAAGAC TCAGAGTGAC
    CTGGAGTACA GCATACCCCC ATCCCCAAAG ACCCCATCAG GCATGATGGA TCATGCCACT
    CTAATGTGCC CAAGCCCTTC CTGGAATGGC CCAAAAGGTT CCACTTTCTC TCCCTCCTGG
    AATGACTCCT ACAACTATGC CTTGGTTACC AGCCCTGCTT CCAAGCAGCC AGTGACTAAA
    ACTGGAATGT TAACACCTGG AGGTGATAGG GGTGGTCTAA TTTGTCCATC TCAGACTAGT
    TCAGGCCTCT CCATGAGTTC AGGGTCACAC TCCAGTTCTT TCACCTCCAT CTCTGAACCA
    TGTGGGCACA GAGTATACAC AGTGTCCACA CAGGGGAGTG GAGTTGTAAC AGGGAAACGG
    AATGAGACTG AGGTGACAGC TTCTACTGCA GTCACAAGAA ATAGCAAAAG TGAGCAGGAA
    AAGAGATCAG CCCGATCCAA TGTCTTCAGG TTCCGTGAGC GCTCACTTTC AACACCAACA
    GACTCTGGAT CATTTTGCTC AGCAGATAAT ATATGTTCTC CAGGCGAGGC TGTCTCTGGG
    GTTCCAGAGG GTGAAAGTTA TGCCTTGCTT TATCCGAGTA ACAGCTCGGA GGATAGCACG
    AGCACTGATA ATGTTTCTGT GACCACAGGT TCAGACTTTC TTCCTGATGG TCGACTAAGG
    TCACGGTCAC GCAGTATCTC ACTAAAGAAA TCTAAAAAAA AGCCACCACC TCCTGTGCGT
    AGTGTATCTC TGATGAAAAA CCTATCAGAA ATCAGAGGAA TGGGCTCCTA CCAAAACGAA
    GGGCACTTCA GGGACGGCAG ACCTAAGAGT CTTTTTATTC CTCGAGATAT TCAAGATTCA
    TTCCAGCCTG AGTTCCTAAT TGCCAAGCCT GATGATGACA CAGATCAGGC TCATAGCAGC
    TTAGAGACAT CTTCTGATAT TTCTCAGCCT GCTGACAGAG AAACAGAGCT CGGGTTTCCT
    CCTCACTGGC AGCTCAATGA GTGGAAGTCC AACAATGATC CTTACCGCTC ATTGTCAGGT
    TCAAGCACAG CCACAGGTAC CACAGTCATT GAGTGCATGA AGGTGCGAGG AAGTTCAGAA
    TCTCTGAATT CACCCTCAAC ATCAAGAGCC ACATCCCCAT CTCAGATGTC AATTGAGGCT
    GAGACCAAGG TATCCCCTTT TAAACCACCA TGCCTCTTGT CACCATCCAG TGGCTACTCA
    AGCCAGTCTG AAACTCCAAC CCCAACTATA TCAAACACCC TTCTCACTGG ACCTTCTCCA
    GTTGGCTGCA AGATGCGGCC AAAGATTCCA GAGCGTAAAT CTTCCTTACC AGCCTCTGCA
    AAGGACAAAT CACGCTCACG TCTTTCATTT GAACTACCTG CAAATGCTCA ATTGGATCTG
    TCCTTTGTCA AACCCAAGCC TAAAGCCAGT AGACGTCACT CTGACACATC TACAGCCAAC
    AAACCAGGTA AACTGAGCCC CAGTCAGTCT TCACTGCCTA TGGTAACCAC AACAGATTTG
    CGAAACATCC GCCTTCGTTC AGTAAGCCGC TCAGAACTTG AGGACAGTCC TGACGGCTCC
    TCTGACATTA TAGAAGAAGA ACAAAGTCGA GATCTCAGCC CTCCTATCAC ACCCTCTAAT
    ACAAAACAGC CAAAGCCACC AGTAGCAATG AAGCCACCTC TCCCAAAGCG ACCCGTAAAC
    TTAACACTTA AATCTCCCTC ATCCTCCCCA TTGACACCTG AATCTCCTCC ATCCTCCCCT
    GTGGACCGAC CTATGCCTGT TGGTAATATT TATATGGTTG TAAGGAAACC CAAACCTAAA
    AGACCAGCTC AGGCATCACT CAGCACATCT GCAATAGCCA CACAAGAGCC CATCCGCAGC
    CATGTATCTA CTTTTCCAGA GTTTGACCTA GAGCATGGGA TTCCCATCGA AGAAGATCTT
    CCATCCCCAA GTAGCCCAGA AAGGGAGGAT AAATGTAAAA CTCTTCCAAG TAGAATGACA
    ATATCTTGTT TGGCAGAATT AGACAAAAGG AAGCCCAAGG TACCACCACC AGTACCAAAA
    AAACCCAGTG TCCTTCTACT TCCCTCTCCA AGCATCCAGA CCAATGGTGG GGCAGAGAGG
    CAAACTCTAC TTTCAGAAAG CATCTCTCAG TCACCTACCG GTACATCAGC ATCTGAGATT
    GAGGGAGATT TGCTTAAAGA TGAAGAGGAA GACTATTCTG CCAAGCAAAT TTCACATGAA
    AACCACCAAA TTCCTGAACT TTATGAGACC TTTGGGAACG AGGAGAATGA TGTAGTAGTT
    GAAGAATACA GTTCAGAAGA CAGAGGTTCT CATCATTTTA TGGAAAGGGA GACACCTCCA
    GCAGAGATAG TAAAAGAAAT TTACACAGAA ACAGAAATAT TGGAAGAGAA CATCTCTGCA
    GTTGACAAAA CTGAACTACA CATTACAGAG GAAACAGAGG ATGAAGTATT TGTCAACACA
    CCCACAACTC ACACCACTGA AGATCTCTTC ACCATAATAC ACAGGTCAAA GAGAAAAGTT
    CTTGGTCGAA AGGAACCATC AGATTCTTTT GGAAGAAGTC AGTGTCTGGT CTCGCCTGTG
    AAAAGCAGTA GTACAGACTT CAGAACTCTG GGCCTTGGGA CTACACCTCG GTCAAGCTCA
    CGAAATGAAA ACTTTATGGC TCTCCTCCAA AAGAGGAGCA GCAAAGCTAG CAGTGGGACC
    AGAGTTTCTG CCATGGAGTT GCTGAAGAGC ACAAACCCTT TGGCACGACG AGTCACGGAA
    TTCTCTCAGT CACAACCAGA TGGGAGCGCA ATGAACACAC CAAAACCATC TTCGCAGGAC
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001334053.3
    CDS275..3940
    Translation

    Target ORF information:

    RefSeq Version XM_001334017.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001334017.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGTTTTGGG ACAGGAAACA CGGAGCTATG GGCAATTCCC AACGCCGGGC TAAAGGGAGG 
    CATAAAACCA AGGGGGCAAC AGCCGCCACC AACTTGGACT CGGAGAGCAA ACGAACGGCC
    CACTTCCAAT CGTCATGGCA ACAGCATGTG AATGTGTTCG GCTCGTGGAG TAGGCCGGAG
    TGTGTGCAGG AGCTGCACCA GGAGGCTCAG CTCAACCTAC AGAGTCTGCT GCAAGATTTC
    GAGGAGCAGT TGTATGATAA CAGAGTGACA GGACAGACAT TCAAACATCC TTCCTCTCAG
    AGCTCGGAGG ACACGTCAAC AACTCTCAGC CGGTCGCCTT CCTCACTCAA CAACAAGAAG
    ACGGAATTCG TCTTTGTGCC TGCAAATAAG CAGGTGTGTG AGGATGAGAC CACAACTTTA
    GGGGTCCGGG CGCAGGACCC GAGCCCTTGC GCTTCAGAGA AGACTCTTTT AGGCTGGAAT
    GCTGCACTGA GTCCACCTGT TGCAGAGAAA CCTCGCTGGT ACCTTGGCCG ACAAACTCCA
    GCTCAGCTTG TACCCATTGA GATCACAGGC CAGAGCTTTG ATAAGCACGC CGCACGGCAC
    AGCGAACTGC GGGCCACCAT TAGAACTGAT TCAGCGGTCA ACCCAAAAAC CATTCGCCGC
    CCGAGGAGTG TGGTTGCAGG CCCTGATGTC ACGCTGCACT GCCAAGGCGA CAGCAAAATT
    CTTGCTGTTG ATTTGATACA AGGTGCCTGT TCCCCTGGAT CATCTCAACC TAGATCTTTG
    GAGCCTACCA CAGAGAATAC AGGTCAACAG CATATTCCTC TTCGAAAGAC TCAGAGTGAC
    CTGGAGTACA GCATACCCCC ATCCCCAAAG ACCCCATCAG GCATGATGGA TCATGCCACT
    CTAATGTGCC CAAGCCCTTC CTGGAATGGC CCAAAAGGTT CCACTTTCTC TCCCTCCTGG
    AATGACTCCT ACAACTATGC CTTGGTTACC AGCCCTGCTT CCAAGCAGCC AGTGACTAAA
    ACTGGAATGT TAACACCTGG AGGTGATAGG GGTGGTCTAA TTTGTCCATC TCAGACTAGT
    TCAGGCCTCT CCATGAGTTC AGGGTCACAC TCCAGTTCTT TCACCTCCAT CTCTGAACCA
    TGTGGGCACA GAGTATACAC AGTGTCCACA CAGGGGAGTG GAGTTGTAAC AGGGAAACGG
    AATGAGACTG AGGTGACAGC TTCTACTGCA GTCACAAGAA ATAGCAAAAG TGAGCAGGAA
    AAGAGATCAG CCCGATCCAA TGTCTTCAGG TTCCGTGAGC GCTCACTTTC AACACCAACA
    GACTCTGGAT CATTTTGCTC AGCAGATAAT ATATGTTCTC CAGGCGAGGC TGTCTCTGGG
    GTTCCAGAGG GTGAAAGTTA TGCCTTGCTT TATCCGAGTA ACAGCTCGGA GGATAGCACG
    AGCACTGATA ATGTTTCTGT GACCACAGGT TCAGACTTTC TTCCTGATGG TCGACTAAGG
    TCACGGTCAC GCAGTATCTC ACTAAAGAAA TCTAAAAAAA AGCCACCACC TCCTGTGCGT
    AGTGTATCTC TGATGAAAAA CCTATCAGAA ATCAGAGGAA TGGGCTCCTA CCAAAACGAA
    GGGCACTTCA GGGACGGCAG ACCTAAGAGT CTTTTTATTC CTCGAGATAT TCAAGATTCA
    TTCCAGCCTG AGTTCCTAAT TGCCAAGCCT GATGATGACA CAGATCAGGC TCATAGCAGC
    TTAGAGACAT CTTCTGATAT TTCTCAGCCT GCTGACAGAG AAACAGAGCT CGGGTTTCCT
    CCTCACTGGC AGCTCAATGA GTGGAAGTCC AACAATGATC CTTACCGCTC ATTGTCAGGT
    TCAAGCACAG CCACAGGTAC CACAGTCATT GAGTGCATGA AGGTGCGAGG AAGTTCAGAA
    TCTCTGAATT CACCCTCAAC ATCAAGAGCC ACATCCCCAT CTCAGATGTC AATTGAGGCT
    GAGACCAAGG TATCCCCTTT TAAACCACCA TGCCTCTTGT CACCATCCAG TGGCTACTCA
    AGCCAGTCTG AAACTCCAAC CCCAACTATA TCAAACACCC TTCTCACTGG ACCTTCTCCA
    GTTGGCTGCA AGATGCGGCC AAAGATTCCA GAGCGTAAAT CTTCCTTACC AGCCTCTGCA
    AAGGACAAAT CACGCTCACG TCTTTCATTT GAACTACCTG CAAATGCTCA ATTGGATCTG
    TCCTTTGTCA AACCCAAGCC TAAAGCCAGT AGACGTCACT CTGACACATC TACAGCCAAC
    AAACCAGGTA AACTGAGCCC CAGTCAGTCT TCACTGCCTA TGGTAACCAC AACAGATTTG
    CGAAACATCC GCCTTCGTTC AGTAAGCCGC TCAGAACTTG AGGACAGTCC TGACGGCTCC
    TCTGACATTA TAGAAGAAGA ACAAAGTCGA GATCTCAGCC CTCCTATCAC ACCCTCTAAT
    ACAAAACAGC CAAAGCCACC AGTAGCAATG AAGCCACCTC TCCCAAAGCG ACCCGTAAAC
    TTAACACTTA AATCTCCCTC ATCCTCCCCA TTGACACCTG AATCTCCTCC ATCCTCCCCT
    GTGGACCGAC CTATGCCTGT TGGTAATATT TATATGGTTG TAAGGAAACC CAAACCTAAA
    AGACCAGCTC AGGCATCACT CAGCACATCT GCAATAGCCA CACAAGAGCC CATCCGCAGC
    CATGTATCTA CTTTTCCAGA GTTTGACCTA GAGCATGGGA TTCCCATCGA AGAAGATCTT
    CCATCCCCAA GTAGCCCAGA AAGGGAGGAT AAATGTAAAA CTCTTCCAAG TAGAATGACA
    ATATCTTGTT TGGCAGAATT AGACAAAAGG AAGCCCAAGG TACCACCACC AGTACCAAAA
    AAACCCAGTG TCCTTCTACT TCCCTCTCCA AGCATCCAGA CCAATGGTGG GGCAGAGAGG
    CAAACTCTAC TTTCAGAAAG CATCTCTCAG TCACCTACCG GTACATCAGC ATCTGAGATT
    GAGGGAGATT TGCTTAAAGA TGAAGAGGAA GACTATTCTG CCAAGCAAAT TTCACATGAA
    AACCACCAAA TTCCTGAACT TTATGAGACC TTTGGGAACG AGGAGAATGA TGTAGTAGTT
    GAAGAATACA GTTCAGAAGA CAGAGGTTCT CATCATTTTA TGGAAAGGGA GACACCTCCA
    GCAGAGATAG TAAAAGAAAT TTACACAGAA ACAGAAATAT TGGAAGAGAA CATCTCTGCA
    GTTGACAAAA CTGAACTACA CATTACAGAG GAAACAGAGG ATGAAGTATT TGTCAACACA
    CCCACAACTC ACACCACTGA AGATCTCTTC ACCATAATAC ACAGGTCAAA GAGAAAAGTT
    CTTGGTCGAA AGGAACCATC AGATTCTTTT GGAAGAAGTC AGTGTCTGGT CTCGCCTGTG
    AAAAGCAGTA GTACAGACTT CAGAACTCTG GGCCTTGGGA CTACACCTCG GTCAAGCTCA
    CGAAATGAAA ACTTTATGGC TCTCCTCCAA AAGAGGAGCA GCAAAGCTAG CAGTGGGACC
    AGAGTTTCTG CCATGGAGTT GCTGAAGAGC ACAAACCCTT TGGCACGACG AGTCACGGAA
    TTCTCTCAGT CACAACCAGA TGGGAGCGCA ATGAACACAC CAAAACCATC TTCGCAGGAC
    CAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46883
    Clone ID Related Accession (Same CDS sequence) XM_009303449.2 , XM_009303449.3
    Accession Version XM_009303449.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303449.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCCAGAGCT TTGATAAGCA CGCCGCACGG
    CACAGCGAAC TGCGGGCCAC CATTAGAACT GATTCAGCGG TCAACCCAAA AACCATTCGC
    CGCCCGAGGA GTGTGGTTGC AGGCCCTGAT GTCACGCTGC ACTGCCAAGG CGACAGCAAA
    ATTCTTGCTG TTGATTTGAT ACAAGGTGCC TGTTCCCCTG GATCATCTCA ACCTAGATCT
    TTGGAGCCTA CCACAGAGAA TACAGGTCAA CAGCATATTC CTCTTCGAAA GACTCAGAGT
    GACCTGGAGT ACAGCATACC CCCATCCCCA AAGACCCCAT CAGGCATGAT GGATCATGCC
    ACTCTAATGT GCCCAAGCCC TTCCTGGAAT GGCCCAAAAG GTTCCACTTT CTCTCCCTCC
    TGGAATGACT CCTACAACTA TGCCTTGGTT ACCAGCCCTG CTTCCAAGCA GCCAGTGACT
    AAAACTGGAA TGTTAACACC TGGAGGTGAT AGGGGTGGTC TAATTTGTCC ATCTCAGACT
    AGTTCAGGCC TCTCCATGAG TTCAGGGTCA CACTCCAGTT CTTTCACCTC CATCTCTGAA
    CCATGTGGGC ACAGAGTATA CACAGTGTCC ACACAGGGGA GTGGAGTTGT AACAGGGAAA
    CGGAATGAGA CTGAGGTGAC AGCTTCTACT GCAGTCACAA GAAATAGCAA AAGTGAGCAG
    GAAAAGAGAT CAGCCCGATC CAATGTCTTC AGGTTCCGTG AGCGCTCACT TTCAACACCA
    ACAGACTCTG GATCATTTTG CTCAGCAGAT AATATATGTT CTCCAGGCGA GGCTGTCTCT
    GGGGTTCCAG AGGGTGAAAG TTATGCCTTG CTTTATCCGA GTAACAGCTC GGAGGATAGC
    ACGAGCACTG ATAATGTTTC TGTGACCACA GGTTCAGACT TTCTTCCTGA TGGTCGACTA
    AGGTCACGGT CACGCAGTAT CTCACTAAAG AAATCTAAAA AAAAGCCACC ACCTCCTGTG
    CGTAGTGTAT CTCTGATGAA AAACCTATCA GAAATCAGAG GAATGGGCTC CTACCAAAAC
    GAAGGGCACT TCAGGGACGG CAGACCTAAG AGTCTTTTTA TTCCTCGAGA TATTCAAGAT
    TCATTCCAGC CTGAGTTCCT AATTGCCAAG CCTGATGATG ACACAGATCA GGCTCATAGC
    AGCTTAGAGA CATCTTCTGA TATTTCTCAG CCTGCTGACA GAGAAACAGA GCTCGGGTTT
    CCTCCTCACT GGCAGCTCAA TGAGTGGAAG TCCAACAATG ATCCTTACCG CTCATTGTCA
    GGTTCAAGCA CAGCCACAGG TACCACAGTC ATTGAGTGCA TGAAGGTGCG AGGAAGTTCA
    GAATCTCTGA ATTCACCCTC AACATCAAGA GCCACATCCC CATCTCAGAT GTCAATTGAG
    GCTGAGACCA AGGTATCCCC TTTTAAACCA CCATGCCTCT TGTCACCATC CAGTGGCTAC
    TCAAGCCAGT CTGAAACTCC AACCCCAACT ATATCAAACA CCCTTCTCAC TGGACCTTCT
    CCAGTTGGCT GCAAGATGCG GCCAAAGATT CCAGAGCGTA AATCTTCCTT ACCAGCCTCT
    GCAAAGGACA AATCACGCTC ACGTCTTTCA TTTGAACTAC CTGCAAATGC TCAATTGGAT
    CTGTCCTTTG TCAAACCCAA GCCTAAAGCC AGTAGACGTC ACTCTGACAC ATCTACAGCC
    AACAAACCAG GTAAACTGAG CCCCAGTCAG TCTTCACTGC CTATGGTAAC CACAACAGAT
    TTGCGAAACA TCCGCCTTCG TTCAGTAAGC CGCTCAGAAC TTGAGGACAG TCCTGACGGC
    TCCTCTGACA TTATAGAAGA AGAACAAAGT CGAGATCTCA GCCCTCCTAT CACACCCTCT
    AATACAAAAC AGCCAAAGCC ACCAGTAGCA ATGAAGCCAC CTCTCCCAAA GCGACCCGTA
    AACTTAACAC TTAAATCTCC CTCATCCTCC CCATTGACAC CTGAATCTCC TCCATCCTCC
    CCTGTGGACC GACCTATGCC TGTTGGTAAT ATTTATATGG TTGTAAGGAA ACCCAAACCT
    AAAAGACCAG CTCAGGCATC ACTCAGCACA TCTGCAATAG CCACACAAGA GCCCATCCGC
    AGCCATGTAT CTACTTTTCC AGAGTTTGAC CTAGAGCATG GGATTCCCAT CGAAGAAGAT
    CTTCCATCCC CAAGTAGCCC AGAAAGGGAG GATAAATGTA AAACTCTTCC AAGTAGAATG
    ACAATATCTT GTTTGGCAGA ATTAGACAAA AGGAAGCCCA AGGTACCACC ACCAGTACCA
    AAAAAACCCA GTGTCCTTCT ACTTCCCTCT CCAAGCATCC AGACCAATGG TGGGGCAGAG
    AGGCAAACTC TACTTTCAGA AAGCATCTCT CAGTCACCTA CCGGTACATC AGCATCTGAG
    ATTGAGGGAG ATTTGCTTAA AGATGAAGAG GAAGACTATT CTGCCAAGCA AATTTCACAT
    GAAAACCACC AAATTCCTGA ACTTTATGAG ACCTTTGGGA ACGAGGAGAA TGATGTAGTA
    GTTGAAGAAT ACAGTTCAGA AGACAGAGGT TCTCATCATT TTATGGAAAG GGAGACACCT
    CCAGCAGAGA TAGTAAAAGA AATTTACACA GAAACAGAAA TATTGGAAGA GAACATCTCT
    GCAGTTGACA AAACTGAACT ACACATTACA GAGGAAACAG AGGATGAAGT ATTTGTCAAC
    ACACCCACAA CTCACACCAC TGAAGATCTC TTCACCATAA TACACAGGTC AAAGAGAAAA
    GTTCTTGGTC GAAAGGAACC ATCAGATTCT TTTGGAAGAA GTCAGTGTCT GGTCTCGCCT
    GTGAAAAGCA GTAGTACAGA CTTCAGAACT CTGGGCCTTG GGACTACACC TCGGTCAAGC
    TCACGAAATG AAAACTTTAT GGCTCTCCTC CAAAAGAGGA GCAGCAAAGC TAGCAGTGGG
    ACCAGAGTTT CTGCCATGGA GTTGCTGAAG AGCACAAACC CTTTGGCACG ACGAGTCACG
    GAATTCTCTC AGTCACAACC AGATGGGAGC GCAATGAACA CACCAAAACC ATCTTCGCAG
    GACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301724.1
    CDS387..4235
    Translation

    Target ORF information:

    RefSeq Version XM_009303449.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303449.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCCAGAGCT TTGATAAGCA CGCCGCACGG
    CACAGCGAAC TGCGGGCCAC CATTAGAACT GATTCAGCGG TCAACCCAAA AACCATTCGC
    CGCCCGAGGA GTGTGGTTGC AGGCCCTGAT GTCACGCTGC ACTGCCAAGG CGACAGCAAA
    ATTCTTGCTG TTGATTTGAT ACAAGGTGCC TGTTCCCCTG GATCATCTCA ACCTAGATCT
    TTGGAGCCTA CCACAGAGAA TACAGGTCAA CAGCATATTC CTCTTCGAAA GACTCAGAGT
    GACCTGGAGT ACAGCATACC CCCATCCCCA AAGACCCCAT CAGGCATGAT GGATCATGCC
    ACTCTAATGT GCCCAAGCCC TTCCTGGAAT GGCCCAAAAG GTTCCACTTT CTCTCCCTCC
    TGGAATGACT CCTACAACTA TGCCTTGGTT ACCAGCCCTG CTTCCAAGCA GCCAGTGACT
    AAAACTGGAA TGTTAACACC TGGAGGTGAT AGGGGTGGTC TAATTTGTCC ATCTCAGACT
    AGTTCAGGCC TCTCCATGAG TTCAGGGTCA CACTCCAGTT CTTTCACCTC CATCTCTGAA
    CCATGTGGGC ACAGAGTATA CACAGTGTCC ACACAGGGGA GTGGAGTTGT AACAGGGAAA
    CGGAATGAGA CTGAGGTGAC AGCTTCTACT GCAGTCACAA GAAATAGCAA AAGTGAGCAG
    GAAAAGAGAT CAGCCCGATC CAATGTCTTC AGGTTCCGTG AGCGCTCACT TTCAACACCA
    ACAGACTCTG GATCATTTTG CTCAGCAGAT AATATATGTT CTCCAGGCGA GGCTGTCTCT
    GGGGTTCCAG AGGGTGAAAG TTATGCCTTG CTTTATCCGA GTAACAGCTC GGAGGATAGC
    ACGAGCACTG ATAATGTTTC TGTGACCACA GGTTCAGACT TTCTTCCTGA TGGTCGACTA
    AGGTCACGGT CACGCAGTAT CTCACTAAAG AAATCTAAAA AAAAGCCACC ACCTCCTGTG
    CGTAGTGTAT CTCTGATGAA AAACCTATCA GAAATCAGAG GAATGGGCTC CTACCAAAAC
    GAAGGGCACT TCAGGGACGG CAGACCTAAG AGTCTTTTTA TTCCTCGAGA TATTCAAGAT
    TCATTCCAGC CTGAGTTCCT AATTGCCAAG CCTGATGATG ACACAGATCA GGCTCATAGC
    AGCTTAGAGA CATCTTCTGA TATTTCTCAG CCTGCTGACA GAGAAACAGA GCTCGGGTTT
    CCTCCTCACT GGCAGCTCAA TGAGTGGAAG TCCAACAATG ATCCTTACCG CTCATTGTCA
    GGTTCAAGCA CAGCCACAGG TACCACAGTC ATTGAGTGCA TGAAGGTGCG AGGAAGTTCA
    GAATCTCTGA ATTCACCCTC AACATCAAGA GCCACATCCC CATCTCAGAT GTCAATTGAG
    GCTGAGACCA AGGTATCCCC TTTTAAACCA CCATGCCTCT TGTCACCATC CAGTGGCTAC
    TCAAGCCAGT CTGAAACTCC AACCCCAACT ATATCAAACA CCCTTCTCAC TGGACCTTCT
    CCAGTTGGCT GCAAGATGCG GCCAAAGATT CCAGAGCGTA AATCTTCCTT ACCAGCCTCT
    GCAAAGGACA AATCACGCTC ACGTCTTTCA TTTGAACTAC CTGCAAATGC TCAATTGGAT
    CTGTCCTTTG TCAAACCCAA GCCTAAAGCC AGTAGACGTC ACTCTGACAC ATCTACAGCC
    AACAAACCAG GTAAACTGAG CCCCAGTCAG TCTTCACTGC CTATGGTAAC CACAACAGAT
    TTGCGAAACA TCCGCCTTCG TTCAGTAAGC CGCTCAGAAC TTGAGGACAG TCCTGACGGC
    TCCTCTGACA TTATAGAAGA AGAACAAAGT CGAGATCTCA GCCCTCCTAT CACACCCTCT
    AATACAAAAC AGCCAAAGCC ACCAGTAGCA ATGAAGCCAC CTCTCCCAAA GCGACCCGTA
    AACTTAACAC TTAAATCTCC CTCATCCTCC CCATTGACAC CTGAATCTCC TCCATCCTCC
    CCTGTGGACC GACCTATGCC TGTTGGTAAT ATTTATATGG TTGTAAGGAA ACCCAAACCT
    AAAAGACCAG CTCAGGCATC ACTCAGCACA TCTGCAATAG CCACACAAGA GCCCATCCGC
    AGCCATGTAT CTACTTTTCC AGAGTTTGAC CTAGAGCATG GGATTCCCAT CGAAGAAGAT
    CTTCCATCCC CAAGTAGCCC AGAAAGGGAG GATAAATGTA AAACTCTTCC AAGTAGAATG
    ACAATATCTT GTTTGGCAGA ATTAGACAAA AGGAAGCCCA AGGTACCACC ACCAGTACCA
    AAAAAACCCA GTGTCCTTCT ACTTCCCTCT CCAAGCATCC AGACCAATGG TGGGGCAGAG
    AGGCAAACTC TACTTTCAGA AAGCATCTCT CAGTCACCTA CCGGTACATC AGCATCTGAG
    ATTGAGGGAG ATTTGCTTAA AGATGAAGAG GAAGACTATT CTGCCAAGCA AATTTCACAT
    GAAAACCACC AAATTCCTGA ACTTTATGAG ACCTTTGGGA ACGAGGAGAA TGATGTAGTA
    GTTGAAGAAT ACAGTTCAGA AGACAGAGGT TCTCATCATT TTATGGAAAG GGAGACACCT
    CCAGCAGAGA TAGTAAAAGA AATTTACACA GAAACAGAAA TATTGGAAGA GAACATCTCT
    GCAGTTGACA AAACTGAACT ACACATTACA GAGGAAACAG AGGATGAAGT ATTTGTCAAC
    ACACCCACAA CTCACACCAC TGAAGATCTC TTCACCATAA TACACAGGTC AAAGAGAAAA
    GTTCTTGGTC GAAAGGAACC ATCAGATTCT TTTGGAAGAA GTCAGTGTCT GGTCTCGCCT
    GTGAAAAGCA GTAGTACAGA CTTCAGAACT CTGGGCCTTG GGACTACACC TCGGTCAAGC
    TCACGAAATG AAAACTTTAT GGCTCTCCTC CAAAAGAGGA GCAGCAAAGC TAGCAGTGGG
    ACCAGAGTTT CTGCCATGGA GTTGCTGAAG AGCACAAACC CTTTGGCACG ACGAGTCACG
    GAATTCTCTC AGTCACAACC AGATGGGAGC GCAATGAACA CACCAAAACC ATCTTCGCAG
    GACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46883
    Clone ID Related Accession (Same CDS sequence) XM_009303449.2 , XM_009303449.3
    Accession Version XM_009303449.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3849bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303449.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCCAGAGCT TTGATAAGCA CGCCGCACGG
    CACAGCGAAC TGCGGGCCAC CATTAGAACT GATTCAGCGG TCAACCCAAA AACCATTCGC
    CGCCCGAGGA GTGTGGTTGC AGGCCCTGAT GTCACGCTGC ACTGCCAAGG CGACAGCAAA
    ATTCTTGCTG TTGATTTGAT ACAAGGTGCC TGTTCCCCTG GATCATCTCA ACCTAGATCT
    TTGGAGCCTA CCACAGAGAA TACAGGTCAA CAGCATATTC CTCTTCGAAA GACTCAGAGT
    GACCTGGAGT ACAGCATACC CCCATCCCCA AAGACCCCAT CAGGCATGAT GGATCATGCC
    ACTCTAATGT GCCCAAGCCC TTCCTGGAAT GGCCCAAAAG GTTCCACTTT CTCTCCCTCC
    TGGAATGACT CCTACAACTA TGCCTTGGTT ACCAGCCCTG CTTCCAAGCA GCCAGTGACT
    AAAACTGGAA TGTTAACACC TGGAGGTGAT AGGGGTGGTC TAATTTGTCC ATCTCAGACT
    AGTTCAGGCC TCTCCATGAG TTCAGGGTCA CACTCCAGTT CTTTCACCTC CATCTCTGAA
    CCATGTGGGC ACAGAGTATA CACAGTGTCC ACACAGGGGA GTGGAGTTGT AACAGGGAAA
    CGGAATGAGA CTGAGGTGAC AGCTTCTACT GCAGTCACAA GAAATAGCAA AAGTGAGCAG
    GAAAAGAGAT CAGCCCGATC CAATGTCTTC AGGTTCCGTG AGCGCTCACT TTCAACACCA
    ACAGACTCTG GATCATTTTG CTCAGCAGAT AATATATGTT CTCCAGGCGA GGCTGTCTCT
    GGGGTTCCAG AGGGTGAAAG TTATGCCTTG CTTTATCCGA GTAACAGCTC GGAGGATAGC
    ACGAGCACTG ATAATGTTTC TGTGACCACA GGTTCAGACT TTCTTCCTGA TGGTCGACTA
    AGGTCACGGT CACGCAGTAT CTCACTAAAG AAATCTAAAA AAAAGCCACC ACCTCCTGTG
    CGTAGTGTAT CTCTGATGAA AAACCTATCA GAAATCAGAG GAATGGGCTC CTACCAAAAC
    GAAGGGCACT TCAGGGACGG CAGACCTAAG AGTCTTTTTA TTCCTCGAGA TATTCAAGAT
    TCATTCCAGC CTGAGTTCCT AATTGCCAAG CCTGATGATG ACACAGATCA GGCTCATAGC
    AGCTTAGAGA CATCTTCTGA TATTTCTCAG CCTGCTGACA GAGAAACAGA GCTCGGGTTT
    CCTCCTCACT GGCAGCTCAA TGAGTGGAAG TCCAACAATG ATCCTTACCG CTCATTGTCA
    GGTTCAAGCA CAGCCACAGG TACCACAGTC ATTGAGTGCA TGAAGGTGCG AGGAAGTTCA
    GAATCTCTGA ATTCACCCTC AACATCAAGA GCCACATCCC CATCTCAGAT GTCAATTGAG
    GCTGAGACCA AGGTATCCCC TTTTAAACCA CCATGCCTCT TGTCACCATC CAGTGGCTAC
    TCAAGCCAGT CTGAAACTCC AACCCCAACT ATATCAAACA CCCTTCTCAC TGGACCTTCT
    CCAGTTGGCT GCAAGATGCG GCCAAAGATT CCAGAGCGTA AATCTTCCTT ACCAGCCTCT
    GCAAAGGACA AATCACGCTC ACGTCTTTCA TTTGAACTAC CTGCAAATGC TCAATTGGAT
    CTGTCCTTTG TCAAACCCAA GCCTAAAGCC AGTAGACGTC ACTCTGACAC ATCTACAGCC
    AACAAACCAG GTAAACTGAG CCCCAGTCAG TCTTCACTGC CTATGGTAAC CACAACAGAT
    TTGCGAAACA TCCGCCTTCG TTCAGTAAGC CGCTCAGAAC TTGAGGACAG TCCTGACGGC
    TCCTCTGACA TTATAGAAGA AGAACAAAGT CGAGATCTCA GCCCTCCTAT CACACCCTCT
    AATACAAAAC AGCCAAAGCC ACCAGTAGCA ATGAAGCCAC CTCTCCCAAA GCGACCCGTA
    AACTTAACAC TTAAATCTCC CTCATCCTCC CCATTGACAC CTGAATCTCC TCCATCCTCC
    CCTGTGGACC GACCTATGCC TGTTGGTAAT ATTTATATGG TTGTAAGGAA ACCCAAACCT
    AAAAGACCAG CTCAGGCATC ACTCAGCACA TCTGCAATAG CCACACAAGA GCCCATCCGC
    AGCCATGTAT CTACTTTTCC AGAGTTTGAC CTAGAGCATG GGATTCCCAT CGAAGAAGAT
    CTTCCATCCC CAAGTAGCCC AGAAAGGGAG GATAAATGTA AAACTCTTCC AAGTAGAATG
    ACAATATCTT GTTTGGCAGA ATTAGACAAA AGGAAGCCCA AGGTACCACC ACCAGTACCA
    AAAAAACCCA GTGTCCTTCT ACTTCCCTCT CCAAGCATCC AGACCAATGG TGGGGCAGAG
    AGGCAAACTC TACTTTCAGA AAGCATCTCT CAGTCACCTA CCGGTACATC AGCATCTGAG
    ATTGAGGGAG ATTTGCTTAA AGATGAAGAG GAAGACTATT CTGCCAAGCA AATTTCACAT
    GAAAACCACC AAATTCCTGA ACTTTATGAG ACCTTTGGGA ACGAGGAGAA TGATGTAGTA
    GTTGAAGAAT ACAGTTCAGA AGACAGAGGT TCTCATCATT TTATGGAAAG GGAGACACCT
    CCAGCAGAGA TAGTAAAAGA AATTTACACA GAAACAGAAA TATTGGAAGA GAACATCTCT
    GCAGTTGACA AAACTGAACT ACACATTACA GAGGAAACAG AGGATGAAGT ATTTGTCAAC
    ACACCCACAA CTCACACCAC TGAAGATCTC TTCACCATAA TACACAGGTC AAAGAGAAAA
    GTTCTTGGTC GAAAGGAACC ATCAGATTCT TTTGGAAGAA GTCAGTGTCT GGTCTCGCCT
    GTGAAAAGCA GTAGTACAGA CTTCAGAACT CTGGGCCTTG GGACTACACC TCGGTCAAGC
    TCACGAAATG AAAACTTTAT GGCTCTCCTC CAAAAGAGGA GCAGCAAAGC TAGCAGTGGG
    ACCAGAGTTT CTGCCATGGA GTTGCTGAAG AGCACAAACC CTTTGGCACG ACGAGTCACG
    GAATTCTCTC AGTCACAACC AGATGGGAGC GCAATGAACA CACCAAAACC ATCTTCGCAG
    GACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301724.1
    CDS387..4235
    Translation

    Target ORF information:

    RefSeq Version XM_009303449.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303449.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCCAGAGCT TTGATAAGCA CGCCGCACGG
    CACAGCGAAC TGCGGGCCAC CATTAGAACT GATTCAGCGG TCAACCCAAA AACCATTCGC
    CGCCCGAGGA GTGTGGTTGC AGGCCCTGAT GTCACGCTGC ACTGCCAAGG CGACAGCAAA
    ATTCTTGCTG TTGATTTGAT ACAAGGTGCC TGTTCCCCTG GATCATCTCA ACCTAGATCT
    TTGGAGCCTA CCACAGAGAA TACAGGTCAA CAGCATATTC CTCTTCGAAA GACTCAGAGT
    GACCTGGAGT ACAGCATACC CCCATCCCCA AAGACCCCAT CAGGCATGAT GGATCATGCC
    ACTCTAATGT GCCCAAGCCC TTCCTGGAAT GGCCCAAAAG GTTCCACTTT CTCTCCCTCC
    TGGAATGACT CCTACAACTA TGCCTTGGTT ACCAGCCCTG CTTCCAAGCA GCCAGTGACT
    AAAACTGGAA TGTTAACACC TGGAGGTGAT AGGGGTGGTC TAATTTGTCC ATCTCAGACT
    AGTTCAGGCC TCTCCATGAG TTCAGGGTCA CACTCCAGTT CTTTCACCTC CATCTCTGAA
    CCATGTGGGC ACAGAGTATA CACAGTGTCC ACACAGGGGA GTGGAGTTGT AACAGGGAAA
    CGGAATGAGA CTGAGGTGAC AGCTTCTACT GCAGTCACAA GAAATAGCAA AAGTGAGCAG
    GAAAAGAGAT CAGCCCGATC CAATGTCTTC AGGTTCCGTG AGCGCTCACT TTCAACACCA
    ACAGACTCTG GATCATTTTG CTCAGCAGAT AATATATGTT CTCCAGGCGA GGCTGTCTCT
    GGGGTTCCAG AGGGTGAAAG TTATGCCTTG CTTTATCCGA GTAACAGCTC GGAGGATAGC
    ACGAGCACTG ATAATGTTTC TGTGACCACA GGTTCAGACT TTCTTCCTGA TGGTCGACTA
    AGGTCACGGT CACGCAGTAT CTCACTAAAG AAATCTAAAA AAAAGCCACC ACCTCCTGTG
    CGTAGTGTAT CTCTGATGAA AAACCTATCA GAAATCAGAG GAATGGGCTC CTACCAAAAC
    GAAGGGCACT TCAGGGACGG CAGACCTAAG AGTCTTTTTA TTCCTCGAGA TATTCAAGAT
    TCATTCCAGC CTGAGTTCCT AATTGCCAAG CCTGATGATG ACACAGATCA GGCTCATAGC
    AGCTTAGAGA CATCTTCTGA TATTTCTCAG CCTGCTGACA GAGAAACAGA GCTCGGGTTT
    CCTCCTCACT GGCAGCTCAA TGAGTGGAAG TCCAACAATG ATCCTTACCG CTCATTGTCA
    GGTTCAAGCA CAGCCACAGG TACCACAGTC ATTGAGTGCA TGAAGGTGCG AGGAAGTTCA
    GAATCTCTGA ATTCACCCTC AACATCAAGA GCCACATCCC CATCTCAGAT GTCAATTGAG
    GCTGAGACCA AGGTATCCCC TTTTAAACCA CCATGCCTCT TGTCACCATC CAGTGGCTAC
    TCAAGCCAGT CTGAAACTCC AACCCCAACT ATATCAAACA CCCTTCTCAC TGGACCTTCT
    CCAGTTGGCT GCAAGATGCG GCCAAAGATT CCAGAGCGTA AATCTTCCTT ACCAGCCTCT
    GCAAAGGACA AATCACGCTC ACGTCTTTCA TTTGAACTAC CTGCAAATGC TCAATTGGAT
    CTGTCCTTTG TCAAACCCAA GCCTAAAGCC AGTAGACGTC ACTCTGACAC ATCTACAGCC
    AACAAACCAG GTAAACTGAG CCCCAGTCAG TCTTCACTGC CTATGGTAAC CACAACAGAT
    TTGCGAAACA TCCGCCTTCG TTCAGTAAGC CGCTCAGAAC TTGAGGACAG TCCTGACGGC
    TCCTCTGACA TTATAGAAGA AGAACAAAGT CGAGATCTCA GCCCTCCTAT CACACCCTCT
    AATACAAAAC AGCCAAAGCC ACCAGTAGCA ATGAAGCCAC CTCTCCCAAA GCGACCCGTA
    AACTTAACAC TTAAATCTCC CTCATCCTCC CCATTGACAC CTGAATCTCC TCCATCCTCC
    CCTGTGGACC GACCTATGCC TGTTGGTAAT ATTTATATGG TTGTAAGGAA ACCCAAACCT
    AAAAGACCAG CTCAGGCATC ACTCAGCACA TCTGCAATAG CCACACAAGA GCCCATCCGC
    AGCCATGTAT CTACTTTTCC AGAGTTTGAC CTAGAGCATG GGATTCCCAT CGAAGAAGAT
    CTTCCATCCC CAAGTAGCCC AGAAAGGGAG GATAAATGTA AAACTCTTCC AAGTAGAATG
    ACAATATCTT GTTTGGCAGA ATTAGACAAA AGGAAGCCCA AGGTACCACC ACCAGTACCA
    AAAAAACCCA GTGTCCTTCT ACTTCCCTCT CCAAGCATCC AGACCAATGG TGGGGCAGAG
    AGGCAAACTC TACTTTCAGA AAGCATCTCT CAGTCACCTA CCGGTACATC AGCATCTGAG
    ATTGAGGGAG ATTTGCTTAA AGATGAAGAG GAAGACTATT CTGCCAAGCA AATTTCACAT
    GAAAACCACC AAATTCCTGA ACTTTATGAG ACCTTTGGGA ACGAGGAGAA TGATGTAGTA
    GTTGAAGAAT ACAGTTCAGA AGACAGAGGT TCTCATCATT TTATGGAAAG GGAGACACCT
    CCAGCAGAGA TAGTAAAAGA AATTTACACA GAAACAGAAA TATTGGAAGA GAACATCTCT
    GCAGTTGACA AAACTGAACT ACACATTACA GAGGAAACAG AGGATGAAGT ATTTGTCAAC
    ACACCCACAA CTCACACCAC TGAAGATCTC TTCACCATAA TACACAGGTC AAAGAGAAAA
    GTTCTTGGTC GAAAGGAACC ATCAGATTCT TTTGGAAGAA GTCAGTGTCT GGTCTCGCCT
    GTGAAAAGCA GTAGTACAGA CTTCAGAACT CTGGGCCTTG GGACTACACC TCGGTCAAGC
    TCACGAAATG AAAACTTTAT GGCTCTCCTC CAAAAGAGGA GCAGCAAAGC TAGCAGTGGG
    ACCAGAGTTT CTGCCATGGA GTTGCTGAAG AGCACAAACC CTTTGGCACG ACGAGTCACG
    GAATTCTCTC AGTCACAACC AGATGGGAGC GCAATGAACA CACCAAAACC ATCTTCGCAG
    GACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46884
    Clone ID Related Accession (Same CDS sequence) XM_009303450.3 , XM_009303450.2
    Accession Version XM_009303450.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCGACAGCA AAATTCTTGC TGTTGATTTG
    ATACAAGGTG CCTGTTCCCC TGGATCATCT CAACCTAGAT CTTTGGAGCC TACCACAGAG
    AATACAGGTC AACAGCATAT TCCTCTTCGA AAGACTCAGA GTGACCTGGA GTACAGCATA
    CCCCCATCCC CAAAGACCCC ATCAGGCATG ATGGATCATG CCACTCTAAT GTGCCCAAGC
    CCTTCCTGGA ATGGCCCAAA AGGTTCCACT TTCTCTCCCT CCTGGAATGA CTCCTACAAC
    TATGCCTTGG TTACCAGCCC TGCTTCCAAG CAGCCAGTGA CTAAAACTGG AATGTTAACA
    CCTGGAGGTG ATAGGGGTGG TCTAATTTGT CCATCTCAGA CTAGTTCAGG CCTCTCCATG
    AGTTCAGGGT CACACTCCAG TTCTTTCACC TCCATCTCTG AACCATGTGG GCACAGAGTA
    TACACAGTGT CCACACAGGG GAGTGGAGTT GTAACAGGGA AACGGAATGA GACTGAGGTG
    ACAGCTTCTA CTGCAGTCAC AAGAAATAGC AAAAGTGAGC AGGAAAAGAG ATCAGCCCGA
    TCCAATGTCT TCAGGTTCCG TGAGCGCTCA CTTTCAACAC CAACAGACTC TGGATCATTT
    TGCTCAGCAG ATAATATATG TTCTCCAGGC GAGGCTGTCT CTGGGGTTCC AGAGGGTGAA
    AGTTATGCCT TGCTTTATCC GAGTAACAGC TCGGAGGATA GCACGAGCAC TGATAATGTT
    TCTGTGACCA CAGGTTCAGA CTTTCTTCCT GATGGTCGAC TAAGGTCACG GTCACGCAGT
    ATCTCACTAA AGAAATCTAA AAAAAAGCCA CCACCTCCTG TGCGTAGTGT ATCTCTGATG
    AAAAACCTAT CAGAAATCAG AGGAATGGGC TCCTACCAAA ACGAAGGGCA CTTCAGGGAC
    GGCAGACCTA AGAGTCTTTT TATTCCTCGA GATATTCAAG ATTCATTCCA GCCTGAGTTC
    CTAATTGCCA AGCCTGATGA TGACACAGAT CAGGCTCATA GCAGCTTAGA GACATCTTCT
    GATATTTCTC AGCCTGCTGA CAGAGAAACA GAGCTCGGGT TTCCTCCTCA CTGGCAGCTC
    AATGAGTGGA AGTCCAACAA TGATCCTTAC CGCTCATTGT CAGGTTCAAG CACAGCCACA
    GGTACCACAG TCATTGAGTG CATGAAGGTG CGAGGAAGTT CAGAATCTCT GAATTCACCC
    TCAACATCAA GAGCCACATC CCCATCTCAG ATGTCAATTG AGGCTGAGAC CAAGGTATCC
    CCTTTTAAAC CACCATGCCT CTTGTCACCA TCCAGTGGCT ACTCAAGCCA GTCTGAAACT
    CCAACCCCAA CTATATCAAA CACCCTTCTC ACTGGACCTT CTCCAGTTGG CTGCAAGATG
    CGGCCAAAGA TTCCAGAGCG TAAATCTTCC TTACCAGCCT CTGCAAAGGA CAAATCACGC
    TCACGTCTTT CATTTGAACT ACCTGCAAAT GCTCAATTGG ATCTGTCCTT TGTCAAACCC
    AAGCCTAAAG CCAGTAGACG TCACTCTGAC ACATCTACAG CCAACAAACC AGGTAAACTG
    AGCCCCAGTC AGTCTTCACT GCCTATGGTA ACCACAACAG ATTTGCGAAA CATCCGCCTT
    CGTTCAGTAA GCCGCTCAGA ACTTGAGGAC AGTCCTGACG GCTCCTCTGA CATTATAGAA
    GAAGAACAAA GTCGAGATCT CAGCCCTCCT ATCACACCCT CTAATACAAA ACAGCCAAAG
    CCACCAGTAG CAATGAAGCC ACCTCTCCCA AAGCGACCCG TAAACTTAAC ACTTAAATCT
    CCCTCATCCT CCCCATTGAC ACCTGAATCT CCTCCATCCT CCCCTGTGGA CCGACCTATG
    CCTGTTGGTA ATATTTATAT GGTTGTAAGG AAACCCAAAC CTAAAAGACC AGCTCAGGCA
    TCACTCAGCA CATCTGCAAT AGCCACACAA GAGCCCATCC GCAGCCATGT ATCTACTTTT
    CCAGAGTTTG ACCTAGAGCA TGGGATTCCC ATCGAAGAAG ATCTTCCATC CCCAAGTAGC
    CCAGAAAGGG AGGATAAATG TAAAACTCTT CCAAGTAGAA TGACAATATC TTGTTTGGCA
    GAATTAGACA AAAGGAAGCC CAAGGTACCA CCACCAGTAC CAAAAAAACC CAGTGTCCTT
    CTACTTCCCT CTCCAAGCAT CCAGACCAAT GGTGGGGCAG AGAGGCAAAC TCTACTTTCA
    GAAAGCATCT CTCAGTCACC TACCGGTACA TCAGCATCTG AGATTGAGGG AGATTTGCTT
    AAAGATGAAG AGGAAGACTA TTCTGCCAAG CAAATTTCAC ATGAAAACCA CCAAATTCCT
    GAACTTTATG AGACCTTTGG GAACGAGGAG AATGATGTAG TAGTTGAAGA ATACAGTTCA
    GAAGACAGAG GTTCTCATCA TTTTATGGAA AGGGAGACAC CTCCAGCAGA GATAGTAAAA
    GAAATTTACA CAGAAACAGA AATATTGGAA GAGAACATCT CTGCAGTTGA CAAAACTGAA
    CTACACATTA CAGAGGAAAC AGAGGATGAA GTATTTGTCA ACACACCCAC AACTCACACC
    ACTGAAGATC TCTTCACCAT AATACACAGG TCAAAGAGAA AAGTTCTTGG TCGAAAGGAA
    CCATCAGATT CTTTTGGAAG AAGTCAGTGT CTGGTCTCGC CTGTGAAAAG CAGTAGTACA
    GACTTCAGAA CTCTGGGCCT TGGGACTACA CCTCGGTCAA GCTCACGAAA TGAAAACTTT
    ATGGCTCTCC TCCAAAAGAG GAGCAGCAAA GCTAGCAGTG GGACCAGAGT TTCTGCCATG
    GAGTTGCTGA AGAGCACAAA CCCTTTGGCA CGACGAGTCA CGGAATTCTC TCAGTCACAA
    CCAGATGGGA GCGCAATGAA CACACCAAAA CCATCTTCGC AGGACCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301725.1
    CDS387..4097
    Translation

    Target ORF information:

    RefSeq Version XM_009303450.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303450.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCGACAGCA AAATTCTTGC TGTTGATTTG
    ATACAAGGTG CCTGTTCCCC TGGATCATCT CAACCTAGAT CTTTGGAGCC TACCACAGAG
    AATACAGGTC AACAGCATAT TCCTCTTCGA AAGACTCAGA GTGACCTGGA GTACAGCATA
    CCCCCATCCC CAAAGACCCC ATCAGGCATG ATGGATCATG CCACTCTAAT GTGCCCAAGC
    CCTTCCTGGA ATGGCCCAAA AGGTTCCACT TTCTCTCCCT CCTGGAATGA CTCCTACAAC
    TATGCCTTGG TTACCAGCCC TGCTTCCAAG CAGCCAGTGA CTAAAACTGG AATGTTAACA
    CCTGGAGGTG ATAGGGGTGG TCTAATTTGT CCATCTCAGA CTAGTTCAGG CCTCTCCATG
    AGTTCAGGGT CACACTCCAG TTCTTTCACC TCCATCTCTG AACCATGTGG GCACAGAGTA
    TACACAGTGT CCACACAGGG GAGTGGAGTT GTAACAGGGA AACGGAATGA GACTGAGGTG
    ACAGCTTCTA CTGCAGTCAC AAGAAATAGC AAAAGTGAGC AGGAAAAGAG ATCAGCCCGA
    TCCAATGTCT TCAGGTTCCG TGAGCGCTCA CTTTCAACAC CAACAGACTC TGGATCATTT
    TGCTCAGCAG ATAATATATG TTCTCCAGGC GAGGCTGTCT CTGGGGTTCC AGAGGGTGAA
    AGTTATGCCT TGCTTTATCC GAGTAACAGC TCGGAGGATA GCACGAGCAC TGATAATGTT
    TCTGTGACCA CAGGTTCAGA CTTTCTTCCT GATGGTCGAC TAAGGTCACG GTCACGCAGT
    ATCTCACTAA AGAAATCTAA AAAAAAGCCA CCACCTCCTG TGCGTAGTGT ATCTCTGATG
    AAAAACCTAT CAGAAATCAG AGGAATGGGC TCCTACCAAA ACGAAGGGCA CTTCAGGGAC
    GGCAGACCTA AGAGTCTTTT TATTCCTCGA GATATTCAAG ATTCATTCCA GCCTGAGTTC
    CTAATTGCCA AGCCTGATGA TGACACAGAT CAGGCTCATA GCAGCTTAGA GACATCTTCT
    GATATTTCTC AGCCTGCTGA CAGAGAAACA GAGCTCGGGT TTCCTCCTCA CTGGCAGCTC
    AATGAGTGGA AGTCCAACAA TGATCCTTAC CGCTCATTGT CAGGTTCAAG CACAGCCACA
    GGTACCACAG TCATTGAGTG CATGAAGGTG CGAGGAAGTT CAGAATCTCT GAATTCACCC
    TCAACATCAA GAGCCACATC CCCATCTCAG ATGTCAATTG AGGCTGAGAC CAAGGTATCC
    CCTTTTAAAC CACCATGCCT CTTGTCACCA TCCAGTGGCT ACTCAAGCCA GTCTGAAACT
    CCAACCCCAA CTATATCAAA CACCCTTCTC ACTGGACCTT CTCCAGTTGG CTGCAAGATG
    CGGCCAAAGA TTCCAGAGCG TAAATCTTCC TTACCAGCCT CTGCAAAGGA CAAATCACGC
    TCACGTCTTT CATTTGAACT ACCTGCAAAT GCTCAATTGG ATCTGTCCTT TGTCAAACCC
    AAGCCTAAAG CCAGTAGACG TCACTCTGAC ACATCTACAG CCAACAAACC AGGTAAACTG
    AGCCCCAGTC AGTCTTCACT GCCTATGGTA ACCACAACAG ATTTGCGAAA CATCCGCCTT
    CGTTCAGTAA GCCGCTCAGA ACTTGAGGAC AGTCCTGACG GCTCCTCTGA CATTATAGAA
    GAAGAACAAA GTCGAGATCT CAGCCCTCCT ATCACACCCT CTAATACAAA ACAGCCAAAG
    CCACCAGTAG CAATGAAGCC ACCTCTCCCA AAGCGACCCG TAAACTTAAC ACTTAAATCT
    CCCTCATCCT CCCCATTGAC ACCTGAATCT CCTCCATCCT CCCCTGTGGA CCGACCTATG
    CCTGTTGGTA ATATTTATAT GGTTGTAAGG AAACCCAAAC CTAAAAGACC AGCTCAGGCA
    TCACTCAGCA CATCTGCAAT AGCCACACAA GAGCCCATCC GCAGCCATGT ATCTACTTTT
    CCAGAGTTTG ACCTAGAGCA TGGGATTCCC ATCGAAGAAG ATCTTCCATC CCCAAGTAGC
    CCAGAAAGGG AGGATAAATG TAAAACTCTT CCAAGTAGAA TGACAATATC TTGTTTGGCA
    GAATTAGACA AAAGGAAGCC CAAGGTACCA CCACCAGTAC CAAAAAAACC CAGTGTCCTT
    CTACTTCCCT CTCCAAGCAT CCAGACCAAT GGTGGGGCAG AGAGGCAAAC TCTACTTTCA
    GAAAGCATCT CTCAGTCACC TACCGGTACA TCAGCATCTG AGATTGAGGG AGATTTGCTT
    AAAGATGAAG AGGAAGACTA TTCTGCCAAG CAAATTTCAC ATGAAAACCA CCAAATTCCT
    GAACTTTATG AGACCTTTGG GAACGAGGAG AATGATGTAG TAGTTGAAGA ATACAGTTCA
    GAAGACAGAG GTTCTCATCA TTTTATGGAA AGGGAGACAC CTCCAGCAGA GATAGTAAAA
    GAAATTTACA CAGAAACAGA AATATTGGAA GAGAACATCT CTGCAGTTGA CAAAACTGAA
    CTACACATTA CAGAGGAAAC AGAGGATGAA GTATTTGTCA ACACACCCAC AACTCACACC
    ACTGAAGATC TCTTCACCAT AATACACAGG TCAAAGAGAA AAGTTCTTGG TCGAAAGGAA
    CCATCAGATT CTTTTGGAAG AAGTCAGTGT CTGGTCTCGC CTGTGAAAAG CAGTAGTACA
    GACTTCAGAA CTCTGGGCCT TGGGACTACA CCTCGGTCAA GCTCACGAAA TGAAAACTTT
    ATGGCTCTCC TCCAAAAGAG GAGCAGCAAA GCTAGCAGTG GGACCAGAGT TTCTGCCATG
    GAGTTGCTGA AGAGCACAAA CCCTTTGGCA CGACGAGTCA CGGAATTCTC TCAGTCACAA
    CCAGATGGGA GCGCAATGAA CACACCAAAA CCATCTTCGC AGGACCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46884
    Clone ID Related Accession (Same CDS sequence) XM_009303450.3 , XM_009303450.2
    Accession Version XM_009303450.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3711bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCGACAGCA AAATTCTTGC TGTTGATTTG
    ATACAAGGTG CCTGTTCCCC TGGATCATCT CAACCTAGAT CTTTGGAGCC TACCACAGAG
    AATACAGGTC AACAGCATAT TCCTCTTCGA AAGACTCAGA GTGACCTGGA GTACAGCATA
    CCCCCATCCC CAAAGACCCC ATCAGGCATG ATGGATCATG CCACTCTAAT GTGCCCAAGC
    CCTTCCTGGA ATGGCCCAAA AGGTTCCACT TTCTCTCCCT CCTGGAATGA CTCCTACAAC
    TATGCCTTGG TTACCAGCCC TGCTTCCAAG CAGCCAGTGA CTAAAACTGG AATGTTAACA
    CCTGGAGGTG ATAGGGGTGG TCTAATTTGT CCATCTCAGA CTAGTTCAGG CCTCTCCATG
    AGTTCAGGGT CACACTCCAG TTCTTTCACC TCCATCTCTG AACCATGTGG GCACAGAGTA
    TACACAGTGT CCACACAGGG GAGTGGAGTT GTAACAGGGA AACGGAATGA GACTGAGGTG
    ACAGCTTCTA CTGCAGTCAC AAGAAATAGC AAAAGTGAGC AGGAAAAGAG ATCAGCCCGA
    TCCAATGTCT TCAGGTTCCG TGAGCGCTCA CTTTCAACAC CAACAGACTC TGGATCATTT
    TGCTCAGCAG ATAATATATG TTCTCCAGGC GAGGCTGTCT CTGGGGTTCC AGAGGGTGAA
    AGTTATGCCT TGCTTTATCC GAGTAACAGC TCGGAGGATA GCACGAGCAC TGATAATGTT
    TCTGTGACCA CAGGTTCAGA CTTTCTTCCT GATGGTCGAC TAAGGTCACG GTCACGCAGT
    ATCTCACTAA AGAAATCTAA AAAAAAGCCA CCACCTCCTG TGCGTAGTGT ATCTCTGATG
    AAAAACCTAT CAGAAATCAG AGGAATGGGC TCCTACCAAA ACGAAGGGCA CTTCAGGGAC
    GGCAGACCTA AGAGTCTTTT TATTCCTCGA GATATTCAAG ATTCATTCCA GCCTGAGTTC
    CTAATTGCCA AGCCTGATGA TGACACAGAT CAGGCTCATA GCAGCTTAGA GACATCTTCT
    GATATTTCTC AGCCTGCTGA CAGAGAAACA GAGCTCGGGT TTCCTCCTCA CTGGCAGCTC
    AATGAGTGGA AGTCCAACAA TGATCCTTAC CGCTCATTGT CAGGTTCAAG CACAGCCACA
    GGTACCACAG TCATTGAGTG CATGAAGGTG CGAGGAAGTT CAGAATCTCT GAATTCACCC
    TCAACATCAA GAGCCACATC CCCATCTCAG ATGTCAATTG AGGCTGAGAC CAAGGTATCC
    CCTTTTAAAC CACCATGCCT CTTGTCACCA TCCAGTGGCT ACTCAAGCCA GTCTGAAACT
    CCAACCCCAA CTATATCAAA CACCCTTCTC ACTGGACCTT CTCCAGTTGG CTGCAAGATG
    CGGCCAAAGA TTCCAGAGCG TAAATCTTCC TTACCAGCCT CTGCAAAGGA CAAATCACGC
    TCACGTCTTT CATTTGAACT ACCTGCAAAT GCTCAATTGG ATCTGTCCTT TGTCAAACCC
    AAGCCTAAAG CCAGTAGACG TCACTCTGAC ACATCTACAG CCAACAAACC AGGTAAACTG
    AGCCCCAGTC AGTCTTCACT GCCTATGGTA ACCACAACAG ATTTGCGAAA CATCCGCCTT
    CGTTCAGTAA GCCGCTCAGA ACTTGAGGAC AGTCCTGACG GCTCCTCTGA CATTATAGAA
    GAAGAACAAA GTCGAGATCT CAGCCCTCCT ATCACACCCT CTAATACAAA ACAGCCAAAG
    CCACCAGTAG CAATGAAGCC ACCTCTCCCA AAGCGACCCG TAAACTTAAC ACTTAAATCT
    CCCTCATCCT CCCCATTGAC ACCTGAATCT CCTCCATCCT CCCCTGTGGA CCGACCTATG
    CCTGTTGGTA ATATTTATAT GGTTGTAAGG AAACCCAAAC CTAAAAGACC AGCTCAGGCA
    TCACTCAGCA CATCTGCAAT AGCCACACAA GAGCCCATCC GCAGCCATGT ATCTACTTTT
    CCAGAGTTTG ACCTAGAGCA TGGGATTCCC ATCGAAGAAG ATCTTCCATC CCCAAGTAGC
    CCAGAAAGGG AGGATAAATG TAAAACTCTT CCAAGTAGAA TGACAATATC TTGTTTGGCA
    GAATTAGACA AAAGGAAGCC CAAGGTACCA CCACCAGTAC CAAAAAAACC CAGTGTCCTT
    CTACTTCCCT CTCCAAGCAT CCAGACCAAT GGTGGGGCAG AGAGGCAAAC TCTACTTTCA
    GAAAGCATCT CTCAGTCACC TACCGGTACA TCAGCATCTG AGATTGAGGG AGATTTGCTT
    AAAGATGAAG AGGAAGACTA TTCTGCCAAG CAAATTTCAC ATGAAAACCA CCAAATTCCT
    GAACTTTATG AGACCTTTGG GAACGAGGAG AATGATGTAG TAGTTGAAGA ATACAGTTCA
    GAAGACAGAG GTTCTCATCA TTTTATGGAA AGGGAGACAC CTCCAGCAGA GATAGTAAAA
    GAAATTTACA CAGAAACAGA AATATTGGAA GAGAACATCT CTGCAGTTGA CAAAACTGAA
    CTACACATTA CAGAGGAAAC AGAGGATGAA GTATTTGTCA ACACACCCAC AACTCACACC
    ACTGAAGATC TCTTCACCAT AATACACAGG TCAAAGAGAA AAGTTCTTGG TCGAAAGGAA
    CCATCAGATT CTTTTGGAAG AAGTCAGTGT CTGGTCTCGC CTGTGAAAAG CAGTAGTACA
    GACTTCAGAA CTCTGGGCCT TGGGACTACA CCTCGGTCAA GCTCACGAAA TGAAAACTTT
    ATGGCTCTCC TCCAAAAGAG GAGCAGCAAA GCTAGCAGTG GGACCAGAGT TTCTGCCATG
    GAGTTGCTGA AGAGCACAAA CCCTTTGGCA CGACGAGTCA CGGAATTCTC TCAGTCACAA
    CCAGATGGGA GCGCAATGAA CACACCAAAA CCATCTTCGC AGGACCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301725.1
    CDS387..4097
    Translation

    Target ORF information:

    RefSeq Version XM_009303450.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303450.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGCCCTTCT GCAAGAGGAC CATAGTCCCG AAAGATGTGA GCCGGAGTCG CGGCGCGGTG 
    CTGACGGATC TGGTGGATGT GTGCGGCTTG ACTCTGTGCG CGGTGCTCCG GCAGCTGTCG
    GATTTATCCC GGCAGTCAGT GAGCATCCTG GAGGAGCTGG AAGGAGAGCT CGCCTCCATC
    TGCTACCGCT CCGGAGCGCT GGAGAATAAA CTTACCAGCC TGCAGAAACA CATCGCAGCG
    CTGGCCACCA AGCCGCCGGC CAAAACCGCC ACCAACTTGG ACTCGGAGAG CAAACGAACG
    GCCCACTTCC AATCGTCATG GCAACAGCAT GTGAATGTGT TCGGCTCGTG GAGTAGGCCG
    GAGTGTGTGC AGGAGCTGCA CCAGGAGGCT CAGCTCAACC TACAGAGTCT GCTGCAAGAT
    TTCGAGGAGC AGTTGTATGA TAACAGAGTG ACAGGACAGA CATTCAAACA TCCTTCCTCT
    CAGAGCTCGG AGGACACGTC AACAACTCTC AGCCGGTCGC CTTCCTCACT CAACAACAAG
    AAGACGGAAT TCGTCTTTGT GCCTGCAAAT AAGCAGGTGT GTGAGGATGA GACCACAACT
    TTAGGGGTCC GGGCGCAGGA CCCGAGCCCT TGCGCTTCAG AGAAGACTCT TTTAGGCTGG
    AATGCTGCAC TGAGTCCACC TGTTGCAGAG AAACCTCGCT GGTACCTTGG CCGACAAACT
    CCAGCTCAGC TTGTACCCAT TGAGATCACA GGCGACAGCA AAATTCTTGC TGTTGATTTG
    ATACAAGGTG CCTGTTCCCC TGGATCATCT CAACCTAGAT CTTTGGAGCC TACCACAGAG
    AATACAGGTC AACAGCATAT TCCTCTTCGA AAGACTCAGA GTGACCTGGA GTACAGCATA
    CCCCCATCCC CAAAGACCCC ATCAGGCATG ATGGATCATG CCACTCTAAT GTGCCCAAGC
    CCTTCCTGGA ATGGCCCAAA AGGTTCCACT TTCTCTCCCT CCTGGAATGA CTCCTACAAC
    TATGCCTTGG TTACCAGCCC TGCTTCCAAG CAGCCAGTGA CTAAAACTGG AATGTTAACA
    CCTGGAGGTG ATAGGGGTGG TCTAATTTGT CCATCTCAGA CTAGTTCAGG CCTCTCCATG
    AGTTCAGGGT CACACTCCAG TTCTTTCACC TCCATCTCTG AACCATGTGG GCACAGAGTA
    TACACAGTGT CCACACAGGG GAGTGGAGTT GTAACAGGGA AACGGAATGA GACTGAGGTG
    ACAGCTTCTA CTGCAGTCAC AAGAAATAGC AAAAGTGAGC AGGAAAAGAG ATCAGCCCGA
    TCCAATGTCT TCAGGTTCCG TGAGCGCTCA CTTTCAACAC CAACAGACTC TGGATCATTT
    TGCTCAGCAG ATAATATATG TTCTCCAGGC GAGGCTGTCT CTGGGGTTCC AGAGGGTGAA
    AGTTATGCCT TGCTTTATCC GAGTAACAGC TCGGAGGATA GCACGAGCAC TGATAATGTT
    TCTGTGACCA CAGGTTCAGA CTTTCTTCCT GATGGTCGAC TAAGGTCACG GTCACGCAGT
    ATCTCACTAA AGAAATCTAA AAAAAAGCCA CCACCTCCTG TGCGTAGTGT ATCTCTGATG
    AAAAACCTAT CAGAAATCAG AGGAATGGGC TCCTACCAAA ACGAAGGGCA CTTCAGGGAC
    GGCAGACCTA AGAGTCTTTT TATTCCTCGA GATATTCAAG ATTCATTCCA GCCTGAGTTC
    CTAATTGCCA AGCCTGATGA TGACACAGAT CAGGCTCATA GCAGCTTAGA GACATCTTCT
    GATATTTCTC AGCCTGCTGA CAGAGAAACA GAGCTCGGGT TTCCTCCTCA CTGGCAGCTC
    AATGAGTGGA AGTCCAACAA TGATCCTTAC CGCTCATTGT CAGGTTCAAG CACAGCCACA
    GGTACCACAG TCATTGAGTG CATGAAGGTG CGAGGAAGTT CAGAATCTCT GAATTCACCC
    TCAACATCAA GAGCCACATC CCCATCTCAG ATGTCAATTG AGGCTGAGAC CAAGGTATCC
    CCTTTTAAAC CACCATGCCT CTTGTCACCA TCCAGTGGCT ACTCAAGCCA GTCTGAAACT
    CCAACCCCAA CTATATCAAA CACCCTTCTC ACTGGACCTT CTCCAGTTGG CTGCAAGATG
    CGGCCAAAGA TTCCAGAGCG TAAATCTTCC TTACCAGCCT CTGCAAAGGA CAAATCACGC
    TCACGTCTTT CATTTGAACT ACCTGCAAAT GCTCAATTGG ATCTGTCCTT TGTCAAACCC
    AAGCCTAAAG CCAGTAGACG TCACTCTGAC ACATCTACAG CCAACAAACC AGGTAAACTG
    AGCCCCAGTC AGTCTTCACT GCCTATGGTA ACCACAACAG ATTTGCGAAA CATCCGCCTT
    CGTTCAGTAA GCCGCTCAGA ACTTGAGGAC AGTCCTGACG GCTCCTCTGA CATTATAGAA
    GAAGAACAAA GTCGAGATCT CAGCCCTCCT ATCACACCCT CTAATACAAA ACAGCCAAAG
    CCACCAGTAG CAATGAAGCC ACCTCTCCCA AAGCGACCCG TAAACTTAAC ACTTAAATCT
    CCCTCATCCT CCCCATTGAC ACCTGAATCT CCTCCATCCT CCCCTGTGGA CCGACCTATG
    CCTGTTGGTA ATATTTATAT GGTTGTAAGG AAACCCAAAC CTAAAAGACC AGCTCAGGCA
    TCACTCAGCA CATCTGCAAT AGCCACACAA GAGCCCATCC GCAGCCATGT ATCTACTTTT
    CCAGAGTTTG ACCTAGAGCA TGGGATTCCC ATCGAAGAAG ATCTTCCATC CCCAAGTAGC
    CCAGAAAGGG AGGATAAATG TAAAACTCTT CCAAGTAGAA TGACAATATC TTGTTTGGCA
    GAATTAGACA AAAGGAAGCC CAAGGTACCA CCACCAGTAC CAAAAAAACC CAGTGTCCTT
    CTACTTCCCT CTCCAAGCAT CCAGACCAAT GGTGGGGCAG AGAGGCAAAC TCTACTTTCA
    GAAAGCATCT CTCAGTCACC TACCGGTACA TCAGCATCTG AGATTGAGGG AGATTTGCTT
    AAAGATGAAG AGGAAGACTA TTCTGCCAAG CAAATTTCAC ATGAAAACCA CCAAATTCCT
    GAACTTTATG AGACCTTTGG GAACGAGGAG AATGATGTAG TAGTTGAAGA ATACAGTTCA
    GAAGACAGAG GTTCTCATCA TTTTATGGAA AGGGAGACAC CTCCAGCAGA GATAGTAAAA
    GAAATTTACA CAGAAACAGA AATATTGGAA GAGAACATCT CTGCAGTTGA CAAAACTGAA
    CTACACATTA CAGAGGAAAC AGAGGATGAA GTATTTGTCA ACACACCCAC AACTCACACC
    ACTGAAGATC TCTTCACCAT AATACACAGG TCAAAGAGAA AAGTTCTTGG TCGAAAGGAA
    CCATCAGATT CTTTTGGAAG AAGTCAGTGT CTGGTCTCGC CTGTGAAAAG CAGTAGTACA
    GACTTCAGAA CTCTGGGCCT TGGGACTACA CCTCGGTCAA GCTCACGAAA TGAAAACTTT
    ATGGCTCTCC TCCAAAAGAG GAGCAGCAAA GCTAGCAGTG GGACCAGAGT TTCTGCCATG
    GAGTTGCTGA AGAGCACAAA CCCTTTGGCA CGACGAGTCA CGGAATTCTC TCAGTCACAA
    CCAGATGGGA GCGCAATGAA CACACCAAAA CCATCTTCGC AGGACCAGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46885
    Clone ID Related Accession (Same CDS sequence) XM_009303451.2 , XM_009303451.3
    Accession Version XM_009303451.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NHS-like protein 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTTA TTATGGAGAG GCTAGATGTT TTGAGAGGAA AAACAGCCGC CACCAACTTG 
    GACTCGGAGA GCAAACGAAC GGCCCACTTC CAATCGTCAT GGCAACAGCA TGTGAATGTG
    TTCGGCTCGT GGAGTAGGCC GGAGTGTGTG CAGGAGCTGC ACCAGGAGGC TCAGCTCAAC
    CTACAGAGTC TGCTGCAAGA TTTCGAGGAG CAGTTGTATG ATAACAGAGT GACAGGACAG
    ACATTCAAAC ATCCTTCCTC TCAGAGCTCG GAGGACACGT CAACAACTCT CAGCCGGTCG
    CCTTCCTCAC TCAACAACAA GAAGACGGAA TTCGTCTTTG TGCCTGCAAA TAAGCAGGTG
    TGTGAGGATG AGACCACAAC TTTAGGGGTC CGGGCGCAGG ACCCGAGCCC TTGCGCTTCA
    GAGAAGACTC TTTTAGGCTG GAATGCTGCA CTGAGTCCAC CTGTTGCAGA GAAACCTCGC
    TGGTACCTTG GCCGACAAAC TCCAGCTCAG CTTGTACCCA TTGAGATCAC AGGCCAGAGC
    TTTGATAAGC ACGCCGCACG GCACAGCGAA CTGCGGGCCA CCATTAGAAC TGATTCAGCG
    GTCAACCCAA AAACCATTCG CCGCCCGAGG AGTGTGGTTG CAGGCCCTGA TGTCACGCTG
    CACTGCCAAG GCGACAGCAA AATTCTTGCT GTTGATTTGA TACAAGGTGC CTGTTCCCCT
    GGATCATCTC AACCTAGATC TTTGGAGCCT ACCACAGAGA ATACAGGTCA ACAGCATATT
    CCTCTTCGAA AGACTCAGAG TGACCTGGAG TACAGCATAC CCCCATCCCC AAAGACCCCA
    TCAGGCATGA TGGATCATGC CACTCTAATG TGCCCAAGCC CTTCCTGGAA TGGCCCAAAA
    GGTTCCACTT TCTCTCCCTC CTGGAATGAC TCCTACAACT ATGCCTTGGT TACCAGCCCT
    GCTTCCAAGC AGCCAGTGAC TAAAACTGGA ATGTTAACAC CTGGAGGTGA TAGGGGTGGT
    CTAATTTGTC CATCTCAGAC TAGTTCAGGC CTCTCCATGA GTTCAGGGTC ACACTCCAGT
    TCTTTCACCT CCATCTCTGA ACCATGTGGG CACAGAGTAT ACACAGTGTC CACACAGGGG
    AGTGGAGTTG TAACAGGGAA ACGGAATGAG ACTGAGGTGA CAGCTTCTAC TGCAGTCACA
    AGAAATAGCA AAAGTGAGCA GGAAAAGAGA TCAGCCCGAT CCAATGTCTT CAGGTTCCGT
    GAGCGCTCAC TTTCAACACC AACAGACTCT GGATCATTTT GCTCAGCAGA TAATATATGT
    TCTCCAGGCG AGGCTGTCTC TGGGGTTCCA GAGGGTGAAA GTTATGCCTT GCTTTATCCG
    AGTAACAGCT CGGAGGATAG CACGAGCACT GATAATGTTT CTGTGACCAC AGGTTCAGAC
    TTTCTTCCTG ATGGTCGACT AAGGTCACGG TCACGCAGTA TCTCACTAAA GAAATCTAAA
    AAAAAGCCAC CACCTCCTGT GCGTAGTGTA TCTCTGATGA AAAACCTATC AGAAATCAGA
    GGAATGGGCT CCTACCAAAA CGAAGGGCAC TTCAGGGACG GCAGACCTAA GAGTCTTTTT
    ATTCCTCGAG ATATTCAAGA TTCATTCCAG CCTGAGTTCC TAATTGCCAA GCCTGATGAT
    GACACAGATC AGGCTCATAG CAGCTTAGAG ACATCTTCTG ATATTTCTCA GCCTGCTGAC
    AGAGAAACAG AGCTCGGGTT TCCTCCTCAC TGGCAGCTCA ATGAGTGGAA GTCCAACAAT
    GATCCTTACC GCTCATTGTC AGGTTCAAGC ACAGCCACAG GTACCACAGT CATTGAGTGC
    ATGAAGGTGC GAGGAAGTTC AGAATCTCTG AATTCACCCT CAACATCAAG AGCCACATCC
    CCATCTCAGA TGTCAATTGA GGCTGAGACC AAGGTATCCC CTTTTAAACC ACCATGCCTC
    TTGTCACCAT CCAGTGGCTA CTCAAGCCAG TCTGAAACTC CAACCCCAAC TATATCAAAC
    ACCCTTCTCA CTGGACCTTC TCCAGTTGGC TGCAAGATGC GGCCAAAGAT TCCAGAGCGT
    AAATCTTCCT TACCAGCCTC TGCAAAGGAC AAATCACGCT CACGTCTTTC ATTTGAACTA
    CCTGCAAATG CTCAATTGGA TCTGTCCTTT GTCAAACCCA AGCCTAAAGC CAGTAGACGT
    CACTCTGACA CATCTACAGC CAACAAACCA GGTAAACTGA GCCCCAGTCA GTCTTCACTG
    CCTATGGTAA CCACAACAGA TTTGCGAAAC ATCCGCCTTC GTTCAGTAAG CCGCTCAGAA
    CTTGAGGACA GTCCTGACGG CTCCTCTGAC ATTATAGAAG AAGAACAAAG TCGAGATCTC
    AGCCCTCCTA TCACACCCTC TAATACAAAA CAGCCAAAGC CACCAGTAGC AATGAAGCCA
    CCTCTCCCAA AGCGACCCGT AAACTTAACA CTTAAATCTC CCTCATCCTC CCCATTGACA
    CCTGAATCTC CTCCATCCTC CCCTGTGGAC CGACCTATGC CTGTTGGTAA TATTTATATG
    GTTGTAAGGA AACCCAAACC TAAAAGACCA GCTCAGGCAT CACTCAGCAC ATCTGCAATA
    GCCACACAAG AGCCCATCCG CAGCCATGTA TCTACTTTTC CAGAGTTTGA CCTAGAGCAT
    GGGATTCCCA TCGAAGAAGA TCTTCCATCC CCAAGTAGCC CAGAAAGGGA GGATAAATGT
    AAAACTCTTC CAAGTAGAAT GACAATATCT TGTTTGGCAG AATTAGACAA AAGGAAGCCC
    AAGGTACCAC CACCAGTACC AAAAAAACCC AGTGTCCTTC TACTTCCCTC TCCAAGCATC
    CAGACCAATG GTGGGGCAGA GAGGCAAACT CTACTTTCAG AAAGCATCTC TCAGTCACCT
    ACCGGTACAT CAGCATCTGA GATTGAGGGA GATTTGCTTA AAGATGAAGA GGAAGACTAT
    TCTGCCAAGC AAATTTCACA TGAAAACCAC CAAATTCCTG AACTTTATGA GACCTTTGGG
    AACGAGGAGA ATGATGTAGT AGTTGAAGAA TACAGTTCAG AAGACAGAGG TTCTCATCAT
    TTTATGGAAA GGGAGACACC TCCAGCAGAG ATAGTAAAAG AAATTTACAC AGAAACAGAA
    ATATTGGAAG AGAACATCTC TGCAGTTGAC AAAACTGAAC TACACATTAC AGAGGAAACA
    GAGGATGAAG TATTTGTCAA CACACCCACA ACTCACACCA CTGAAGATCT CTTCACCATA
    ATACACAGGT CAAAGAGAAA AGTTCTTGGT CGAAAGGAAC CATCAGATTC TTTTGGAAGA
    AGTCAGTGTC TGGTCTCGCC TGTGAAAAGC AGTAGTACAG ACTTCAGAAC TCTGGGCCTT
    GGGACTACAC CTCGGTCAAG CTCACGAAAT GAAAACTTTA TGGCTCTCCT CCAAAAGAGG
    AGCAGCAAAG CTAGCAGTGG GACCAGAGTT TCTGCCATGG AGTTGCTGAA GAGCACAAAC
    CCTTTGGCAC GACGAGTCAC GGAATTCTCT CAGTCACAAC CAGATGGGAG CGCAATGAAC
    ACACCAAAAC CATCTTCGCA GGACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301726.1
    CDS124..3753
    Translation

    Target ORF information:

    RefSeq Version XM_009303451.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303451.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTTA TTATGGAGAG GCTAGATGTT TTGAGAGGAA AAACAGCCGC CACCAACTTG 
    GACTCGGAGA GCAAACGAAC GGCCCACTTC CAATCGTCAT GGCAACAGCA TGTGAATGTG
    TTCGGCTCGT GGAGTAGGCC GGAGTGTGTG CAGGAGCTGC ACCAGGAGGC TCAGCTCAAC
    CTACAGAGTC TGCTGCAAGA TTTCGAGGAG CAGTTGTATG ATAACAGAGT GACAGGACAG
    ACATTCAAAC ATCCTTCCTC TCAGAGCTCG GAGGACACGT CAACAACTCT CAGCCGGTCG
    CCTTCCTCAC TCAACAACAA GAAGACGGAA TTCGTCTTTG TGCCTGCAAA TAAGCAGGTG
    TGTGAGGATG AGACCACAAC TTTAGGGGTC CGGGCGCAGG ACCCGAGCCC TTGCGCTTCA
    GAGAAGACTC TTTTAGGCTG GAATGCTGCA CTGAGTCCAC CTGTTGCAGA GAAACCTCGC
    TGGTACCTTG GCCGACAAAC TCCAGCTCAG CTTGTACCCA TTGAGATCAC AGGCCAGAGC
    TTTGATAAGC ACGCCGCACG GCACAGCGAA CTGCGGGCCA CCATTAGAAC TGATTCAGCG
    GTCAACCCAA AAACCATTCG CCGCCCGAGG AGTGTGGTTG CAGGCCCTGA TGTCACGCTG
    CACTGCCAAG GCGACAGCAA AATTCTTGCT GTTGATTTGA TACAAGGTGC CTGTTCCCCT
    GGATCATCTC AACCTAGATC TTTGGAGCCT ACCACAGAGA ATACAGGTCA ACAGCATATT
    CCTCTTCGAA AGACTCAGAG TGACCTGGAG TACAGCATAC CCCCATCCCC AAAGACCCCA
    TCAGGCATGA TGGATCATGC CACTCTAATG TGCCCAAGCC CTTCCTGGAA TGGCCCAAAA
    GGTTCCACTT TCTCTCCCTC CTGGAATGAC TCCTACAACT ATGCCTTGGT TACCAGCCCT
    GCTTCCAAGC AGCCAGTGAC TAAAACTGGA ATGTTAACAC CTGGAGGTGA TAGGGGTGGT
    CTAATTTGTC CATCTCAGAC TAGTTCAGGC CTCTCCATGA GTTCAGGGTC ACACTCCAGT
    TCTTTCACCT CCATCTCTGA ACCATGTGGG CACAGAGTAT ACACAGTGTC CACACAGGGG
    AGTGGAGTTG TAACAGGGAA ACGGAATGAG ACTGAGGTGA CAGCTTCTAC TGCAGTCACA
    AGAAATAGCA AAAGTGAGCA GGAAAAGAGA TCAGCCCGAT CCAATGTCTT CAGGTTCCGT
    GAGCGCTCAC TTTCAACACC AACAGACTCT GGATCATTTT GCTCAGCAGA TAATATATGT
    TCTCCAGGCG AGGCTGTCTC TGGGGTTCCA GAGGGTGAAA GTTATGCCTT GCTTTATCCG
    AGTAACAGCT CGGAGGATAG CACGAGCACT GATAATGTTT CTGTGACCAC AGGTTCAGAC
    TTTCTTCCTG ATGGTCGACT AAGGTCACGG TCACGCAGTA TCTCACTAAA GAAATCTAAA
    AAAAAGCCAC CACCTCCTGT GCGTAGTGTA TCTCTGATGA AAAACCTATC AGAAATCAGA
    GGAATGGGCT CCTACCAAAA CGAAGGGCAC TTCAGGGACG GCAGACCTAA GAGTCTTTTT
    ATTCCTCGAG ATATTCAAGA TTCATTCCAG CCTGAGTTCC TAATTGCCAA GCCTGATGAT
    GACACAGATC AGGCTCATAG CAGCTTAGAG ACATCTTCTG ATATTTCTCA GCCTGCTGAC
    AGAGAAACAG AGCTCGGGTT TCCTCCTCAC TGGCAGCTCA ATGAGTGGAA GTCCAACAAT
    GATCCTTACC GCTCATTGTC AGGTTCAAGC ACAGCCACAG GTACCACAGT CATTGAGTGC
    ATGAAGGTGC GAGGAAGTTC AGAATCTCTG AATTCACCCT CAACATCAAG AGCCACATCC
    CCATCTCAGA TGTCAATTGA GGCTGAGACC AAGGTATCCC CTTTTAAACC ACCATGCCTC
    TTGTCACCAT CCAGTGGCTA CTCAAGCCAG TCTGAAACTC CAACCCCAAC TATATCAAAC
    ACCCTTCTCA CTGGACCTTC TCCAGTTGGC TGCAAGATGC GGCCAAAGAT TCCAGAGCGT
    AAATCTTCCT TACCAGCCTC TGCAAAGGAC AAATCACGCT CACGTCTTTC ATTTGAACTA
    CCTGCAAATG CTCAATTGGA TCTGTCCTTT GTCAAACCCA AGCCTAAAGC CAGTAGACGT
    CACTCTGACA CATCTACAGC CAACAAACCA GGTAAACTGA GCCCCAGTCA GTCTTCACTG
    CCTATGGTAA CCACAACAGA TTTGCGAAAC ATCCGCCTTC GTTCAGTAAG CCGCTCAGAA
    CTTGAGGACA GTCCTGACGG CTCCTCTGAC ATTATAGAAG AAGAACAAAG TCGAGATCTC
    AGCCCTCCTA TCACACCCTC TAATACAAAA CAGCCAAAGC CACCAGTAGC AATGAAGCCA
    CCTCTCCCAA AGCGACCCGT AAACTTAACA CTTAAATCTC CCTCATCCTC CCCATTGACA
    CCTGAATCTC CTCCATCCTC CCCTGTGGAC CGACCTATGC CTGTTGGTAA TATTTATATG
    GTTGTAAGGA AACCCAAACC TAAAAGACCA GCTCAGGCAT CACTCAGCAC ATCTGCAATA
    GCCACACAAG AGCCCATCCG CAGCCATGTA TCTACTTTTC CAGAGTTTGA CCTAGAGCAT
    GGGATTCCCA TCGAAGAAGA TCTTCCATCC CCAAGTAGCC CAGAAAGGGA GGATAAATGT
    AAAACTCTTC CAAGTAGAAT GACAATATCT TGTTTGGCAG AATTAGACAA AAGGAAGCCC
    AAGGTACCAC CACCAGTACC AAAAAAACCC AGTGTCCTTC TACTTCCCTC TCCAAGCATC
    CAGACCAATG GTGGGGCAGA GAGGCAAACT CTACTTTCAG AAAGCATCTC TCAGTCACCT
    ACCGGTACAT CAGCATCTGA GATTGAGGGA GATTTGCTTA AAGATGAAGA GGAAGACTAT
    TCTGCCAAGC AAATTTCACA TGAAAACCAC CAAATTCCTG AACTTTATGA GACCTTTGGG
    AACGAGGAGA ATGATGTAGT AGTTGAAGAA TACAGTTCAG AAGACAGAGG TTCTCATCAT
    TTTATGGAAA GGGAGACACC TCCAGCAGAG ATAGTAAAAG AAATTTACAC AGAAACAGAA
    ATATTGGAAG AGAACATCTC TGCAGTTGAC AAAACTGAAC TACACATTAC AGAGGAAACA
    GAGGATGAAG TATTTGTCAA CACACCCACA ACTCACACCA CTGAAGATCT CTTCACCATA
    ATACACAGGT CAAAGAGAAA AGTTCTTGGT CGAAAGGAAC CATCAGATTC TTTTGGAAGA
    AGTCAGTGTC TGGTCTCGCC TGTGAAAAGC AGTAGTACAG ACTTCAGAAC TCTGGGCCTT
    GGGACTACAC CTCGGTCAAG CTCACGAAAT GAAAACTTTA TGGCTCTCCT CCAAAAGAGG
    AGCAGCAAAG CTAGCAGTGG GACCAGAGTT TCTGCCATGG AGTTGCTGAA GAGCACAAAC
    CCTTTGGCAC GACGAGTCAC GGAATTCTCT CAGTCACAAC CAGATGGGAG CGCAATGAAC
    ACACCAAAAC CATCTTCGCA GGACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa46885
    Clone ID Related Accession (Same CDS sequence) XM_009303451.2 , XM_009303451.3
    Accession Version XM_009303451.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3630bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NHS-like protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTTA TTATGGAGAG GCTAGATGTT TTGAGAGGAA AAACAGCCGC CACCAACTTG 
    GACTCGGAGA GCAAACGAAC GGCCCACTTC CAATCGTCAT GGCAACAGCA TGTGAATGTG
    TTCGGCTCGT GGAGTAGGCC GGAGTGTGTG CAGGAGCTGC ACCAGGAGGC TCAGCTCAAC
    CTACAGAGTC TGCTGCAAGA TTTCGAGGAG CAGTTGTATG ATAACAGAGT GACAGGACAG
    ACATTCAAAC ATCCTTCCTC TCAGAGCTCG GAGGACACGT CAACAACTCT CAGCCGGTCG
    CCTTCCTCAC TCAACAACAA GAAGACGGAA TTCGTCTTTG TGCCTGCAAA TAAGCAGGTG
    TGTGAGGATG AGACCACAAC TTTAGGGGTC CGGGCGCAGG ACCCGAGCCC TTGCGCTTCA
    GAGAAGACTC TTTTAGGCTG GAATGCTGCA CTGAGTCCAC CTGTTGCAGA GAAACCTCGC
    TGGTACCTTG GCCGACAAAC TCCAGCTCAG CTTGTACCCA TTGAGATCAC AGGCCAGAGC
    TTTGATAAGC ACGCCGCACG GCACAGCGAA CTGCGGGCCA CCATTAGAAC TGATTCAGCG
    GTCAACCCAA AAACCATTCG CCGCCCGAGG AGTGTGGTTG CAGGCCCTGA TGTCACGCTG
    CACTGCCAAG GCGACAGCAA AATTCTTGCT GTTGATTTGA TACAAGGTGC CTGTTCCCCT
    GGATCATCTC AACCTAGATC TTTGGAGCCT ACCACAGAGA ATACAGGTCA ACAGCATATT
    CCTCTTCGAA AGACTCAGAG TGACCTGGAG TACAGCATAC CCCCATCCCC AAAGACCCCA
    TCAGGCATGA TGGATCATGC CACTCTAATG TGCCCAAGCC CTTCCTGGAA TGGCCCAAAA
    GGTTCCACTT TCTCTCCCTC CTGGAATGAC TCCTACAACT ATGCCTTGGT TACCAGCCCT
    GCTTCCAAGC AGCCAGTGAC TAAAACTGGA ATGTTAACAC CTGGAGGTGA TAGGGGTGGT
    CTAATTTGTC CATCTCAGAC TAGTTCAGGC CTCTCCATGA GTTCAGGGTC ACACTCCAGT
    TCTTTCACCT CCATCTCTGA ACCATGTGGG CACAGAGTAT ACACAGTGTC CACACAGGGG
    AGTGGAGTTG TAACAGGGAA ACGGAATGAG ACTGAGGTGA CAGCTTCTAC TGCAGTCACA
    AGAAATAGCA AAAGTGAGCA GGAAAAGAGA TCAGCCCGAT CCAATGTCTT CAGGTTCCGT
    GAGCGCTCAC TTTCAACACC AACAGACTCT GGATCATTTT GCTCAGCAGA TAATATATGT
    TCTCCAGGCG AGGCTGTCTC TGGGGTTCCA GAGGGTGAAA GTTATGCCTT GCTTTATCCG
    AGTAACAGCT CGGAGGATAG CACGAGCACT GATAATGTTT CTGTGACCAC AGGTTCAGAC
    TTTCTTCCTG ATGGTCGACT AAGGTCACGG TCACGCAGTA TCTCACTAAA GAAATCTAAA
    AAAAAGCCAC CACCTCCTGT GCGTAGTGTA TCTCTGATGA AAAACCTATC AGAAATCAGA
    GGAATGGGCT CCTACCAAAA CGAAGGGCAC TTCAGGGACG GCAGACCTAA GAGTCTTTTT
    ATTCCTCGAG ATATTCAAGA TTCATTCCAG CCTGAGTTCC TAATTGCCAA GCCTGATGAT
    GACACAGATC AGGCTCATAG CAGCTTAGAG ACATCTTCTG ATATTTCTCA GCCTGCTGAC
    AGAGAAACAG AGCTCGGGTT TCCTCCTCAC TGGCAGCTCA ATGAGTGGAA GTCCAACAAT
    GATCCTTACC GCTCATTGTC AGGTTCAAGC ACAGCCACAG GTACCACAGT CATTGAGTGC
    ATGAAGGTGC GAGGAAGTTC AGAATCTCTG AATTCACCCT CAACATCAAG AGCCACATCC
    CCATCTCAGA TGTCAATTGA GGCTGAGACC AAGGTATCCC CTTTTAAACC ACCATGCCTC
    TTGTCACCAT CCAGTGGCTA CTCAAGCCAG TCTGAAACTC CAACCCCAAC TATATCAAAC
    ACCCTTCTCA CTGGACCTTC TCCAGTTGGC TGCAAGATGC GGCCAAAGAT TCCAGAGCGT
    AAATCTTCCT TACCAGCCTC TGCAAAGGAC AAATCACGCT CACGTCTTTC ATTTGAACTA
    CCTGCAAATG CTCAATTGGA TCTGTCCTTT GTCAAACCCA AGCCTAAAGC CAGTAGACGT
    CACTCTGACA CATCTACAGC CAACAAACCA GGTAAACTGA GCCCCAGTCA GTCTTCACTG
    CCTATGGTAA CCACAACAGA TTTGCGAAAC ATCCGCCTTC GTTCAGTAAG CCGCTCAGAA
    CTTGAGGACA GTCCTGACGG CTCCTCTGAC ATTATAGAAG AAGAACAAAG TCGAGATCTC
    AGCCCTCCTA TCACACCCTC TAATACAAAA CAGCCAAAGC CACCAGTAGC AATGAAGCCA
    CCTCTCCCAA AGCGACCCGT AAACTTAACA CTTAAATCTC CCTCATCCTC CCCATTGACA
    CCTGAATCTC CTCCATCCTC CCCTGTGGAC CGACCTATGC CTGTTGGTAA TATTTATATG
    GTTGTAAGGA AACCCAAACC TAAAAGACCA GCTCAGGCAT CACTCAGCAC ATCTGCAATA
    GCCACACAAG AGCCCATCCG CAGCCATGTA TCTACTTTTC CAGAGTTTGA CCTAGAGCAT
    GGGATTCCCA TCGAAGAAGA TCTTCCATCC CCAAGTAGCC CAGAAAGGGA GGATAAATGT
    AAAACTCTTC CAAGTAGAAT GACAATATCT TGTTTGGCAG AATTAGACAA AAGGAAGCCC
    AAGGTACCAC CACCAGTACC AAAAAAACCC AGTGTCCTTC TACTTCCCTC TCCAAGCATC
    CAGACCAATG GTGGGGCAGA GAGGCAAACT CTACTTTCAG AAAGCATCTC TCAGTCACCT
    ACCGGTACAT CAGCATCTGA GATTGAGGGA GATTTGCTTA AAGATGAAGA GGAAGACTAT
    TCTGCCAAGC AAATTTCACA TGAAAACCAC CAAATTCCTG AACTTTATGA GACCTTTGGG
    AACGAGGAGA ATGATGTAGT AGTTGAAGAA TACAGTTCAG AAGACAGAGG TTCTCATCAT
    TTTATGGAAA GGGAGACACC TCCAGCAGAG ATAGTAAAAG AAATTTACAC AGAAACAGAA
    ATATTGGAAG AGAACATCTC TGCAGTTGAC AAAACTGAAC TACACATTAC AGAGGAAACA
    GAGGATGAAG TATTTGTCAA CACACCCACA ACTCACACCA CTGAAGATCT CTTCACCATA
    ATACACAGGT CAAAGAGAAA AGTTCTTGGT CGAAAGGAAC CATCAGATTC TTTTGGAAGA
    AGTCAGTGTC TGGTCTCGCC TGTGAAAAGC AGTAGTACAG ACTTCAGAAC TCTGGGCCTT
    GGGACTACAC CTCGGTCAAG CTCACGAAAT GAAAACTTTA TGGCTCTCCT CCAAAAGAGG
    AGCAGCAAAG CTAGCAGTGG GACCAGAGTT TCTGCCATGG AGTTGCTGAA GAGCACAAAC
    CCTTTGGCAC GACGAGTCAC GGAATTCTCT CAGTCACAAC CAGATGGGAG CGCAATGAAC
    ACACCAAAAC CATCTTCGCA GGACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301726.1
    CDS125..3754
    Translation

    Target ORF information:

    RefSeq Version XM_009303451.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NHS-like 2 (nhsl2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303451.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGATGTTA TTATGGAGAG GCTAGATGTT TTGAGAGGAA AAACAGCCGC CACCAACTTG 
    GACTCGGAGA GCAAACGAAC GGCCCACTTC CAATCGTCAT GGCAACAGCA TGTGAATGTG
    TTCGGCTCGT GGAGTAGGCC GGAGTGTGTG CAGGAGCTGC ACCAGGAGGC TCAGCTCAAC
    CTACAGAGTC TGCTGCAAGA TTTCGAGGAG CAGTTGTATG ATAACAGAGT GACAGGACAG
    ACATTCAAAC ATCCTTCCTC TCAGAGCTCG GAGGACACGT CAACAACTCT CAGCCGGTCG
    CCTTCCTCAC TCAACAACAA GAAGACGGAA TTCGTCTTTG TGCCTGCAAA TAAGCAGGTG
    TGTGAGGATG AGACCACAAC TTTAGGGGTC CGGGCGCAGG ACCCGAGCCC TTGCGCTTCA
    GAGAAGACTC TTTTAGGCTG GAATGCTGCA CTGAGTCCAC CTGTTGCAGA GAAACCTCGC
    TGGTACCTTG GCCGACAAAC TCCAGCTCAG CTTGTACCCA TTGAGATCAC AGGCCAGAGC
    TTTGATAAGC ACGCCGCACG GCACAGCGAA CTGCGGGCCA CCATTAGAAC TGATTCAGCG
    GTCAACCCAA AAACCATTCG CCGCCCGAGG AGTGTGGTTG CAGGCCCTGA TGTCACGCTG
    CACTGCCAAG GCGACAGCAA AATTCTTGCT GTTGATTTGA TACAAGGTGC CTGTTCCCCT
    GGATCATCTC AACCTAGATC TTTGGAGCCT ACCACAGAGA ATACAGGTCA ACAGCATATT
    CCTCTTCGAA AGACTCAGAG TGACCTGGAG TACAGCATAC CCCCATCCCC AAAGACCCCA
    TCAGGCATGA TGGATCATGC CACTCTAATG TGCCCAAGCC CTTCCTGGAA TGGCCCAAAA
    GGTTCCACTT TCTCTCCCTC CTGGAATGAC TCCTACAACT ATGCCTTGGT TACCAGCCCT
    GCTTCCAAGC AGCCAGTGAC TAAAACTGGA ATGTTAACAC CTGGAGGTGA TAGGGGTGGT
    CTAATTTGTC CATCTCAGAC TAGTTCAGGC CTCTCCATGA GTTCAGGGTC ACACTCCAGT
    TCTTTCACCT CCATCTCTGA ACCATGTGGG CACAGAGTAT ACACAGTGTC CACACAGGGG
    AGTGGAGTTG TAACAGGGAA ACGGAATGAG ACTGAGGTGA CAGCTTCTAC TGCAGTCACA
    AGAAATAGCA AAAGTGAGCA GGAAAAGAGA TCAGCCCGAT CCAATGTCTT CAGGTTCCGT
    GAGCGCTCAC TTTCAACACC AACAGACTCT GGATCATTTT GCTCAGCAGA TAATATATGT
    TCTCCAGGCG AGGCTGTCTC TGGGGTTCCA GAGGGTGAAA GTTATGCCTT GCTTTATCCG
    AGTAACAGCT CGGAGGATAG CACGAGCACT GATAATGTTT CTGTGACCAC AGGTTCAGAC
    TTTCTTCCTG ATGGTCGACT AAGGTCACGG TCACGCAGTA TCTCACTAAA GAAATCTAAA
    AAAAAGCCAC CACCTCCTGT GCGTAGTGTA TCTCTGATGA AAAACCTATC AGAAATCAGA
    GGAATGGGCT CCTACCAAAA CGAAGGGCAC TTCAGGGACG GCAGACCTAA GAGTCTTTTT
    ATTCCTCGAG ATATTCAAGA TTCATTCCAG CCTGAGTTCC TAATTGCCAA GCCTGATGAT
    GACACAGATC AGGCTCATAG CAGCTTAGAG ACATCTTCTG ATATTTCTCA GCCTGCTGAC
    AGAGAAACAG AGCTCGGGTT TCCTCCTCAC TGGCAGCTCA ATGAGTGGAA GTCCAACAAT
    GATCCTTACC GCTCATTGTC AGGTTCAAGC ACAGCCACAG GTACCACAGT CATTGAGTGC
    ATGAAGGTGC GAGGAAGTTC AGAATCTCTG AATTCACCCT CAACATCAAG AGCCACATCC
    CCATCTCAGA TGTCAATTGA GGCTGAGACC AAGGTATCCC CTTTTAAACC ACCATGCCTC
    TTGTCACCAT CCAGTGGCTA CTCAAGCCAG TCTGAAACTC CAACCCCAAC TATATCAAAC
    ACCCTTCTCA CTGGACCTTC TCCAGTTGGC TGCAAGATGC GGCCAAAGAT TCCAGAGCGT
    AAATCTTCCT TACCAGCCTC TGCAAAGGAC AAATCACGCT CACGTCTTTC ATTTGAACTA
    CCTGCAAATG CTCAATTGGA TCTGTCCTTT GTCAAACCCA AGCCTAAAGC CAGTAGACGT
    CACTCTGACA CATCTACAGC CAACAAACCA GGTAAACTGA GCCCCAGTCA GTCTTCACTG
    CCTATGGTAA CCACAACAGA TTTGCGAAAC ATCCGCCTTC GTTCAGTAAG CCGCTCAGAA
    CTTGAGGACA GTCCTGACGG CTCCTCTGAC ATTATAGAAG AAGAACAAAG TCGAGATCTC
    AGCCCTCCTA TCACACCCTC TAATACAAAA CAGCCAAAGC CACCAGTAGC AATGAAGCCA
    CCTCTCCCAA AGCGACCCGT AAACTTAACA CTTAAATCTC CCTCATCCTC CCCATTGACA
    CCTGAATCTC CTCCATCCTC CCCTGTGGAC CGACCTATGC CTGTTGGTAA TATTTATATG
    GTTGTAAGGA AACCCAAACC TAAAAGACCA GCTCAGGCAT CACTCAGCAC ATCTGCAATA
    GCCACACAAG AGCCCATCCG CAGCCATGTA TCTACTTTTC CAGAGTTTGA CCTAGAGCAT
    GGGATTCCCA TCGAAGAAGA TCTTCCATCC CCAAGTAGCC CAGAAAGGGA GGATAAATGT
    AAAACTCTTC CAAGTAGAAT GACAATATCT TGTTTGGCAG AATTAGACAA AAGGAAGCCC
    AAGGTACCAC CACCAGTACC AAAAAAACCC AGTGTCCTTC TACTTCCCTC TCCAAGCATC
    CAGACCAATG GTGGGGCAGA GAGGCAAACT CTACTTTCAG AAAGCATCTC TCAGTCACCT
    ACCGGTACAT CAGCATCTGA GATTGAGGGA GATTTGCTTA AAGATGAAGA GGAAGACTAT
    TCTGCCAAGC AAATTTCACA TGAAAACCAC CAAATTCCTG AACTTTATGA GACCTTTGGG
    AACGAGGAGA ATGATGTAGT AGTTGAAGAA TACAGTTCAG AAGACAGAGG TTCTCATCAT
    TTTATGGAAA GGGAGACACC TCCAGCAGAG ATAGTAAAAG AAATTTACAC AGAAACAGAA
    ATATTGGAAG AGAACATCTC TGCAGTTGAC AAAACTGAAC TACACATTAC AGAGGAAACA
    GAGGATGAAG TATTTGTCAA CACACCCACA ACTCACACCA CTGAAGATCT CTTCACCATA
    ATACACAGGT CAAAGAGAAA AGTTCTTGGT CGAAAGGAAC CATCAGATTC TTTTGGAAGA
    AGTCAGTGTC TGGTCTCGCC TGTGAAAAGC AGTAGTACAG ACTTCAGAAC TCTGGGCCTT
    GGGACTACAC CTCGGTCAAG CTCACGAAAT GAAAACTTTA TGGCTCTCCT CCAAAAGAGG
    AGCAGCAAAG CTAGCAGTGG GACCAGAGTT TCTGCCATGG AGTTGCTGAA GAGCACAAAC
    CCTTTGGCAC GACGAGTCAC GGAATTCTCT CAGTCACAAC CAGATGGGAG CGCAATGAAC
    ACACCAAAAC CATCTTCGCA GGACCAGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.