MED12 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	MED12
Entrez Gene ID	566632
Full Name	mediator complex subunit 12
Synonyms	kgt,kto,mot_m807,shr,trap230
General protein information	Preferred Names mediator complex subunit 12 Names mediator of RNA polymerase II transcription subunit 12 king tut kohtalo motionless ott otter protein kohtalo protein motionless shiri thyroid hormone receptor-associated protein 230 thyroid hormone receptor-associated protein complex 230 kDa component
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001039461.2	NP_001034550.2	mediator of RNA polymerase II transcription subunit 12
XM_009291038.3	XP_009289313.1	mediator of RNA polymerase II transcription subunit 12 isoform X2
XM_009291039.2	XP_009289314.1	mediator of RNA polymerase II transcription subunit 12 isoform X3
XM_009291037.2	XP_009289312.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_009291037.3	XP_009289312.1	mediator of RNA polymerase II transcription subunit 12 isoform X1
XM_009291038.2	XP_009289313.1	mediator of RNA polymerase II transcription subunit 12 isoform X2

Pathways from BioSystems

Homologies

Homo sapiens (human)	MED12	NP_005111.2
	MED12	XP_001088424.2
Rattus norvegicus (Norway rat)	Med12	NP_001180221.1
Danio rerio (zebrafish)	med12	NP_001034550.1
Pan troglodytes (chimpanzee)	MED12	NP_001009019.1
Bos taurus (cattle)	MED12	NP_001192807.1
Xenopus tropicalis (tropical clawed frog)	med12	XP_002934949.2
Gallus gallus (chicken)	MED12	XP_004940691.1
Mus musculus (house mouse)	Med12	NP_067496.2
Canis lupus familiaris (dog)	MED12	XP_003640267.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Nipbl and mediator cooperatively regulate gene expression to control limb development.
Muto A, Ikeda S, Lopez-Burks ME, Kikuchi Y, Calof AL, Lander AD, Schilling TF
PLoS genetics10(9)e1004671(2014 Sep)

Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development.
Wu SY, de Borsetti NH, Bain EJ, Bulow CR, Gamse JT
Developmental biology385(1)13-22(2014 Jan)

Mediator subunit 12 is required for neutrophil development in zebrafish.
Keightley MC, Layton JE, Hayman JW, Heath JK, Lieschke GJ
PloS one6(8)e23845(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

med12 mutants exhibit multiple defects in the development of the epithalamus, including failure of parapineal specification and disruption of cell differentiation and/or maintenance of specific cell types in the pineal organ and the habenula.
Title: Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development.

The kto/med12 mutation results in specific defects of boundary cell formation in the zebrafish hindbrain
Title: The transcriptional mediator component Med12 is required for hindbrain boundary formation.

Mediator subunit 12 is required for neutrophil development in zebrafish.
Title: Mediator subunit 12 is required for neutrophil development in zebrafish.

Med12 modulates the ability of Casanova/Sox32 to induce sox17 expression in endodermal development.
Title: Multiple roles for Med12 in vertebrate endoderm development.

reveal a regulatory role of motionless/Med12 in vertebrate neuronal development
Title: A subunit of the mediator complex regulates vertebrate neuronal development.

The following MED12 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the MED12 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa53311	NM_001039461.2 Latest version!	Danio rerio mediator complex subunit 12 (med12), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44929	XM_009291037.3 Latest version!	Danio rerio mediator complex subunit 12 (med12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44930	XM_009291038.3 Latest version!	Danio rerio mediator complex subunit 12 (med12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44931	XM_009291039.2 Latest version!	Danio rerio mediator complex subunit 12 (med12), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
View Old Accession Versions >>
ODa44929	XM_009291037.2	Danio rerio mediator complex subunit 12 (med12), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa44930	XM_009291038.2	Danio rerio mediator complex subunit 12 (med12), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

MED12 ( NM_001039461.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001034550.2 Danio rerio mediator complex subunit 12 (med12), mRNA.

Price & Availability↑

CloneID	ODa53311
Clone ID Related Accession (Same CDS sequence)	NM_001039461.2
Accession Version	NM_001039461.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6528bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-03-30
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 12
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT956101.9, EB936730.1 and EB890172.1. On Sep 3, 2015 this sequence version replaced NM_001039461.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: DQ133567.1, JN226108.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGATGTTTTC TCCTCCAATG CACTGTGAGT CGAAGGGAAG CCCTTCTCCA 
GAGAAACAAG CCCAAGAGCA GGAGAGCAAA AGCACTGCCA AGGATAAGGG CATGGACCCA 
GCCTTCCCTT TGGTTTATGA ACAGCCCCGC CACATACAGT ACGCCACCCA CTTCCCCATT 
CCTCAGGAGG AGAGTGCAAG TCATGAGTGT AACCAGCGTC TAGTGGTTCT CTATGGAGTC 
GGCAAGCAGC GAGATGAAGC TCGCCACGCC ATCAAGAAGA TCACCAAAGA CATCCTGAAG 
GTTCTCAACC GCAAGAGTAC AGCAGAGACA GGAGGTGAGG AAGGCCAGAA GAGGAAGAGG 
AGCAAACCTG AGGCCTTCCC CACTGCTGAA GATATTTTCT CTAAATTCCA GCACCTCTCA 
CACTTTGACC AGCACCAGGT CACCTCACAG GTGTCCAGGA ATGTTCTGGA GCAAATCACC 
AGCTTTGCCT TAGGGATGTC CTATCACCTC CCACTGGTCC AACACATCCA GTTCATCTTT 
GACCTCATGG AGTACTCTCT TAATATAAGT GGCCTCATTG ACTTTGCTAT ACAGCTGCTG 
AATGAATTGA GTCTGGTGGA AGCAGAACTC CTGCTTAAGT CCTCCAACCT GGCGGGCAGT 
TACACTACAG GCCTCTGTTT GTGTATCGTG GCTGTTCTGC GGAGATACCA CTCCTGCCTC 
ATCCTCAACC CTGACCAGAC CGCACAGGTC TTTGACGGGT TGCGGATTGT AGTCAAGTCT 
GGTGTGAATC CTGCAGACTG TTCCTCAGCA GAACGCTGTA TCCTAGCCTA TCTCTACGAC 
CTCTATACCT CCTGCAGTCA CCTAAAGAGC AAGTTTGGAG AGATTTTCAG TGAGTTCTGC 
TCAAAGGTGA AGAATTCCAT CTATTATAAC ATCGACCCGT CAGACTCCAA CATGCTGTGG 
GATCAGATGT TTATGATCGA TGCCATCACT AATCCTACCG CACACAACCT CAACCACTCC 
ATGTTGGGAA AGATCACCAA TGAACTCAAT GACAGCCCGG CTAACCGCTA CAGCTTTGTG 
TGTAATGTGC TCATGGATGT TTGCGTTGAC CATCGAGATC CTGAGAGGGT GAATGATATT 
GGTATCCTGT GTGCGGAGCT GACAGCATAC TGTCGCTCTC TAAGTGCTGA GTGGTTGGGT 
GTGCTCAAGG CCCTCTGCTG CTCTTCTAAC AACGGCAACT GTGGCTTCAA CGATCTGCTC 
TGCAATGTTG ACGTGAGCGA TCTATCTTTC CACGACTCTC TAGCAACATT CGTTGCCATC 
TTAATAGCTC GGCAGTGTTT GTTGCTGGAA GATCTGGTGC GCTGTGTTGC CATCCCATCC 
CTCCTGAATG CAGCCTGTAG TGAACAGGAT TCTGAACCTG GAGCCAGACT GACATGTAGG 
ATCCTGCTTC ATCTGTTCAG AACCCCACAG CGCAACCCCT GTCCCCAGGA TGGCACTAAA 
TCAGACAAAT CCATAGTGGG GATCAGGTCG TCCTGCGACC GACATCTGCT GGCTGCTTCA 
CAGAACAGCA TCGTGGTGGG AGCCGTTTTT GCTGTATTAA AAGCTGTGTT CATGCTGGGA 
GATGCTGAGC TGAAAGGCTC AGGCTTTTCC CATCCTGCTG GTCTGGATGA TATTGGAGAG 
GATGACATGG GCTCTAAAAA ATCTGGAGGA CGTAATGTCT CTATTGAAAC AGCCAGTCTG 
GTGGTTTACG CCAAGTATGT GCTGAAGAGC ATCTGCCACC AGGAATGGGT TGGCGAGCGC 
TGTCTGAAGT CTCTGTCAGA AGACAGCAGT GCACTGCAGG ATCCAGTGCT GGTCAACATC 
CAGGCTCAGC GCTTGCTGCA GCTCATCTGT TACCCACACC GACAGCTGGA CAGTGAAGAG 
GGTGACAATC CACAGAGACA GCGCATCAAA CGCATCCTGC AGAACATGGA CCAGTGGACC 
ATGAGACAGT CCTCTCTTGA GCTACAACTC ATGATCAAGC AAAGCTCAAA TAATGAGCTG 
TATTCTCTTC TGGAGAACAT AGCCAAGGCC ACTATTGAGG TTTTCCAGAA GTCTGCAGAG 
ATGAACTCTA GTAACCCCTC CTGGAATGGC TCTGCTGTCT CGGGTAGCTC AGTCTCCAAT 
AGCAACAGTG CCAGCAAACT CAAACCTGTG CTAAGTTCCT CTGAGCGTTC AGGTGTCTGG 
TTAGTTGCCC CTCTAATTGG TAAGCTGCCC ACCTCAGTGC AGGGGCACGT GCTCAAGGCT 
GCAGGCGAGG AGCTGGAGAA GGGACAGCAT CTGGGACCCT CTTCACGAAA AGAAAGAGAT 
CGACAGAAGC AAAAAAGCAT GTCTCTCCTG AGCCAGCAGC CCTTCCTGTC ACTGGTTCTG 
ACATGTCTCA AAGGGCAAGA CGAGCAGAGA GAGGGTCTCT TGACGTCCCT CTACAGCCAA 
GTCCAGCAGA TTGTGACTAA CTGGCGAGAG GACCAGTACC AGGACGACTG CAAGGCTAAG 
CAGATGATGC ACGAGGCCTT GAAGCTGCGA CTCAATCTGG TGGGTGGGAT GTTTGACACT 
GTACAGCGTA GCACTCAGCA GACAACAGAG TGGGCAGTGC TTCTGCTGGA CATCATCAGC 
AGTGGCACAG TGGACATGCA GTCTAACAAT GAGCTCTTCA CTACGGTTTT AGACATGCTG 
AGCGTGTTGA TTAACGGGAC TCTGGCGGCT GACATGTCCA GCATATCTCA GGGCAGCATG 
GAGGAGAATA AGAGAGCTTA CATGAACCTG GTCAAGAAGC TCAGGAAAGA ACTTGGTGAC 
CGACAATCCG AGAGCTTGGA GAAAGTGCGT CAGTTGCTGC CTCTGCCCAA GCAGACGCGT 
GATGTCATCA CCTGTGAGCC CCAGGGGTCA CTCATTGACA CAAAGGGCAA CAAAATTGCT 
GGATTTGAAA AAGAGGGTCT TCAGGTATCA ACTAAGCAGA AGATTTCTCC ATGGGATGTG 
TTTGAGGGAC TAAAGCACTC TGCTCCGCTC TCCTGGGGCT GGTTCGGAAC CATACGGGTC 
GACCGCAAAG TCACTAAGTT TGAAGAGCAG CAGCGGCTCC TGCTTTACCA CACACACATC 
AAGCCCAAGC AACGCAGTTA CTACCTGGCG CCCCTCAACC TGCCCCAAGA AGAAGAGGAA 
CCACTCACTC CTGTGCCTCC CGAGCCAGAC AAGAAGCTGG ACACAGCGAA GGTGGAGAAA 
AGCGTCTCCA ATGTCCCGAC TAAGAAAAAG AAAAAGAAAC CTGCATCGAC GGTTAAACAA 
GAGGACTACA AGCCACACAA CACAGTCATG CAGTACGCTC CAGGCATGAC CCCTGATCTC 
CCACTGAGCA TGGGACAAAC AAACATGTCA TATCGAATGG GCTACCAAGC TCCAATGAAC 
ATGTATGCCC AGAACCAGCC TTTACCACCA GGAGGTCCTG GTTTGGAGCC ACCTTTTCGT 
CCTAGGGTTC CTATGAATAA AATGCCTCCA CGTCCCAACT ACACTATGCC TAATATGCAG 
ACCGCTGGCA TGGGCAACCT AATGGGAATG GAGAAGCAGT ACCAGATGAA CTATAAACCC 
ATGCCCAACA TGGCTCAGGG GCAGATGCTC CGTCATCAGC TACAGGGTCA TAATCATTTA 
ATTGGACAGC AGATTAGACA AGTGACACCT AATCCACAGT ATTCATCAAT GCAGCCAGCA 
CAGAATATAC CCCAGGGCTA CACCTCCTAC GGCTCACACA TGGGGATGCA GCCACACCCC 
TCTCAGACCC CTGGAATGGT TCCCAATTCA TATGGAAACC AGGGCTTTCA GACAGGACAT 
CCTGCCACTA ACCCAACTAT GGTGGACTCA ATAAGGCAGA TGCAGCAGAG ACCTGGCTAC 
GTCCATCAGC AGGCTCCTGC GGCATATGGG CACACAATCC AGAGCACACA AAGGTTTCCG 
CATCAATCCA TGCAGCAGAC ACCCATGATG CACGGCTTGG CTCAAAGTCA CTTGGGAGTG 
CAGGGCATCC ACCCAAACAT GAGGCCCAAT CAGATGATGG AGCAGCAGCA GCAACAACAA 
CAACAACAAC AGCAACAGCA GCAACAACAA CAACAACAAC AGGTCCACCA GCAGCAGCAG 
CACATCCGAC AGGGTCCAGT TTTTAGACCA CAGCAGCAAC AGCAGGTTCA GCAGCAAGTG 
CAACAGCAGG TCCAGCAGCA GCAGGTCCAA CAGCAGCAGG TCCAGCAACA GGTTCAGCCT 
CAGCAAGTTC AACAGCAGCA GGTTCAGCAG CAGCAGCAGC AGGTGTCTGC AGCTCAGCCT 
CCCGCTCAGG CCCTGGGCAT GCAGGCTCTG CCCCCGCAAC AGCCCATGTT CCAGCGGCAA 
GGCATGCAGC AAACCCAGCA GCAGCAGCAG ACCGCAGCAC TGGTCAGGCA ACTCCAGCAG 
CAGCTCTCCA GTACTCAACC TGCACAGAAC AACAGCTCCT ATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001034550.2
CDS	72..6599
Translation	MMAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNSSKISSNFSSIIAEKLRCNTFPDTGKRKPQVNQKDNFWLVTARSQSSINNWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYSVPVMRSAWMIKMTCAYHAAITENKMKKRHVIDPCIEWTQIITKYLWEQLQKVAEFYRQSPSQGCGSPLPAPPAEVETAMKQWEYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLRLLLPLLLQYSGEFVQSAYLSRRLAYFCTRRLNLLLSDGSIGPGPGGHQAHGISAQQGNALPPTPTSQPAGGNQPQTPFTDFYICPQHRPVVFGLSCMLQSIVLCCPSALVWHYSLTDSRNKTGSPLDLLPIAPSNLPMPGGNSTFNQQVRAKVREIEEQIKERGQAVEFRWSFDKCQETTAGFTISRVLHTLEVLDNHSFEKSDFSNSLDSLYNRIFGSGQSKDGHEMSPDDDAVVTLLCEWAVSCKRSGPHRAMVVAKLLEKRQTEIEAERCGESEVVDEKGSVSSGSLSAATLPVFQDVLLQFLDTQAPVLTEPGNESERVEFSNLVLLFYELIRHDVFSHNIYMCTLISRGDLASNSHLPRPRSPSDEPSDESERKDQDAGSSVKIEDTGMSESMEIDHNSSANFDEMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESASHECNQRLVVLYGVGKQRDEARHAIKKITKDILKVLNRKSTAETGGEEGQKRKRSKPEAFPTAEDIFSKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSLVEAELLLKSSNLAGSYTTGLCLCIVAVLRRYHSCLILNPDQTAQVFDGLRIVVKSGVNPADCSSAERCILAYLYDLYTSCSHLKSKFGEIFSEFCSKVKNSIYYNIDPSDSNMLWDQMFMIDAITNPTAHNLNHSMLGKITNELNDSPANRYSFVCNVLMDVCVDHRDPERVNDIGILCAELTAYCRSLSAEWLGVLKALCCSSNNGNCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLVRCVAIPSLLNAACSEQDSEPGARLTCRILLHLFRTPQRNPCPQDGTKSDKSIVGIRSSCDRHLLAASQNSIVVGAVFAVLKAVFMLGDAELKGSGFSHPAGLDDIGEDDMGSKKSGGRNVSIETASLVVYAKYVLKSICHQEWVGERCLKSLSEDSSALQDPVLVNIQAQRLLQLICYPHRQLDSEEGDNPQRQRIKRILQNMDQWTMRQSSLELQLMIKQSSNNELYSLLENIAKATIEVFQKSAEMNSSNPSWNGSAVSGSSVSNSNSASKLKPVLSSSERSGVWLVAPLIGKLPTSVQGHVLKAAGEELEKGQHLGPSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQMMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFEKEGLQVSTKQKISPWDVFEGLKHSAPLSWGWFGTIRVDRKVTKFEEQQRLLLYHTHIKPKQRSYYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMSYRMGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQYQMNYKPMPNMAQGQMLRHQLQGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQQQQQVQQQVQQQVQQQQVQQQQVQQQVQPQQVQQQQVQQQQQQVSAAQPPAQALGMQALPPQQPMFQRQGMQQTQQQQQTAALVRQLQQQLSSTQPAQNNSSYY

Target ORF information:

RefSeq Version	NM_001039461.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 12 (med12), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039461.2

ORF Insert Sequence:

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGATGTTTTC TCCTCCAATG CACTGTGAGT CGAAGGGAAG CCCTTCTCCA 
GAGAAACAAG CCCAAGAGCA GGAGAGCAAA AGCACTGCCA AGGATAAGGG CATGGACCCA 
GCCTTCCCTT TGGTTTATGA ACAGCCCCGC CACATACAGT ACGCCACCCA CTTCCCCATT 
CCTCAGGAGG AGAGTGCAAG TCATGAGTGT AACCAGCGTC TAGTGGTTCT CTATGGAGTC 
GGCAAGCAGC GAGATGAAGC TCGCCACGCC ATCAAGAAGA TCACCAAAGA CATCCTGAAG 
GTTCTCAACC GCAAGAGTAC AGCAGAGACA GGAGGTGAGG AAGGCCAGAA GAGGAAGAGG 
AGCAAACCTG AGGCCTTCCC CACTGCTGAA GATATTTTCT CTAAATTCCA GCACCTCTCA 
CACTTTGACC AGCACCAGGT CACCTCACAG GTGTCCAGGA ATGTTCTGGA GCAAATCACC 
AGCTTTGCCT TAGGGATGTC CTATCACCTC CCACTGGTCC AACACATCCA GTTCATCTTT 
GACCTCATGG AGTACTCTCT TAATATAAGT GGCCTCATTG ACTTTGCTAT ACAGCTGCTG 
AATGAATTGA GTCTGGTGGA AGCAGAACTC CTGCTTAAGT CCTCCAACCT GGCGGGCAGT 
TACACTACAG GCCTCTGTTT GTGTATCGTG GCTGTTCTGC GGAGATACCA CTCCTGCCTC 
ATCCTCAACC CTGACCAGAC CGCACAGGTC TTTGACGGGT TGCGGATTGT AGTCAAGTCT 
GGTGTGAATC CTGCAGACTG TTCCTCAGCA GAACGCTGTA TCCTAGCCTA TCTCTACGAC 
CTCTATACCT CCTGCAGTCA CCTAAAGAGC AAGTTTGGAG AGATTTTCAG TGAGTTCTGC 
TCAAAGGTGA AGAATTCCAT CTATTATAAC ATCGACCCGT CAGACTCCAA CATGCTGTGG 
GATCAGATGT TTATGATCGA TGCCATCACT AATCCTACCG CACACAACCT CAACCACTCC 
ATGTTGGGAA AGATCACCAA TGAACTCAAT GACAGCCCGG CTAACCGCTA CAGCTTTGTG 
TGTAATGTGC TCATGGATGT TTGCGTTGAC CATCGAGATC CTGAGAGGGT GAATGATATT 
GGTATCCTGT GTGCGGAGCT GACAGCATAC TGTCGCTCTC TAAGTGCTGA GTGGTTGGGT 
GTGCTCAAGG CCCTCTGCTG CTCTTCTAAC AACGGCAACT GTGGCTTCAA CGATCTGCTC 
TGCAATGTTG ACGTGAGCGA TCTATCTTTC CACGACTCTC TAGCAACATT CGTTGCCATC 
TTAATAGCTC GGCAGTGTTT GTTGCTGGAA GATCTGGTGC GCTGTGTTGC CATCCCATCC 
CTCCTGAATG CAGCCTGTAG TGAACAGGAT TCTGAACCTG GAGCCAGACT GACATGTAGG 
ATCCTGCTTC ATCTGTTCAG AACCCCACAG CGCAACCCCT GTCCCCAGGA TGGCACTAAA 
TCAGACAAAT CCATAGTGGG GATCAGGTCG TCCTGCGACC GACATCTGCT GGCTGCTTCA 
CAGAACAGCA TCGTGGTGGG AGCCGTTTTT GCTGTATTAA AAGCTGTGTT CATGCTGGGA 
GATGCTGAGC TGAAAGGCTC AGGCTTTTCC CATCCTGCTG GTCTGGATGA TATTGGAGAG 
GATGACATGG GCTCTAAAAA ATCTGGAGGA CGTAATGTCT CTATTGAAAC AGCCAGTCTG 
GTGGTTTACG CCAAGTATGT GCTGAAGAGC ATCTGCCACC AGGAATGGGT TGGCGAGCGC 
TGTCTGAAGT CTCTGTCAGA AGACAGCAGT GCACTGCAGG ATCCAGTGCT GGTCAACATC 
CAGGCTCAGC GCTTGCTGCA GCTCATCTGT TACCCACACC GACAGCTGGA CAGTGAAGAG 
GGTGACAATC CACAGAGACA GCGCATCAAA CGCATCCTGC AGAACATGGA CCAGTGGACC 
ATGAGACAGT CCTCTCTTGA GCTACAACTC ATGATCAAGC AAAGCTCAAA TAATGAGCTG 
TATTCTCTTC TGGAGAACAT AGCCAAGGCC ACTATTGAGG TTTTCCAGAA GTCTGCAGAG 
ATGAACTCTA GTAACCCCTC CTGGAATGGC TCTGCTGTCT CGGGTAGCTC AGTCTCCAAT 
AGCAACAGTG CCAGCAAACT CAAACCTGTG CTAAGTTCCT CTGAGCGTTC AGGTGTCTGG 
TTAGTTGCCC CTCTAATTGG TAAGCTGCCC ACCTCAGTGC AGGGGCACGT GCTCAAGGCT 
GCAGGCGAGG AGCTGGAGAA GGGACAGCAT CTGGGACCCT CTTCACGAAA AGAAAGAGAT 
CGACAGAAGC AAAAAAGCAT GTCTCTCCTG AGCCAGCAGC CCTTCCTGTC ACTGGTTCTG 
ACATGTCTCA AAGGGCAAGA CGAGCAGAGA GAGGGTCTCT TGACGTCCCT CTACAGCCAA 
GTCCAGCAGA TTGTGACTAA CTGGCGAGAG GACCAGTACC AGGACGACTG CAAGGCTAAG 
CAGATGATGC ACGAGGCCTT GAAGCTGCGA CTCAATCTGG TGGGTGGGAT GTTTGACACT 
GTACAGCGTA GCACTCAGCA GACAACAGAG TGGGCAGTGC TTCTGCTGGA CATCATCAGC 
AGTGGCACAG TGGACATGCA GTCTAACAAT GAGCTCTTCA CTACGGTTTT AGACATGCTG 
AGCGTGTTGA TTAACGGGAC TCTGGCGGCT GACATGTCCA GCATATCTCA GGGCAGCATG 
GAGGAGAATA AGAGAGCTTA CATGAACCTG GTCAAGAAGC TCAGGAAAGA ACTTGGTGAC 
CGACAATCCG AGAGCTTGGA GAAAGTGCGT CAGTTGCTGC CTCTGCCCAA GCAGACGCGT 
GATGTCATCA CCTGTGAGCC CCAGGGGTCA CTCATTGACA CAAAGGGCAA CAAAATTGCT 
GGATTTGAAA AAGAGGGTCT TCAGGTATCA ACTAAGCAGA AGATTTCTCC ATGGGATGTG 
TTTGAGGGAC TAAAGCACTC TGCTCCGCTC TCCTGGGGCT GGTTCGGAAC CATACGGGTC 
GACCGCAAAG TCACTAAGTT TGAAGAGCAG CAGCGGCTCC TGCTTTACCA CACACACATC 
AAGCCCAAGC AACGCAGTTA CTACCTGGCG CCCCTCAACC TGCCCCAAGA AGAAGAGGAA 
CCACTCACTC CTGTGCCTCC CGAGCCAGAC AAGAAGCTGG ACACAGCGAA GGTGGAGAAA 
AGCGTCTCCA ATGTCCCGAC TAAGAAAAAG AAAAAGAAAC CTGCATCGAC GGTTAAACAA 
GAGGACTACA AGCCACACAA CACAGTCATG CAGTACGCTC CAGGCATGAC CCCTGATCTC 
CCACTGAGCA TGGGACAAAC AAACATGTCA TATCGAATGG GCTACCAAGC TCCAATGAAC 
ATGTATGCCC AGAACCAGCC TTTACCACCA GGAGGTCCTG GTTTGGAGCC ACCTTTTCGT 
CCTAGGGTTC CTATGAATAA AATGCCTCCA CGTCCCAACT ACACTATGCC TAATATGCAG 
ACCGCTGGCA TGGGCAACCT AATGGGAATG GAGAAGCAGT ACCAGATGAA CTATAAACCC 
ATGCCCAACA TGGCTCAGGG GCAGATGCTC CGTCATCAGC TACAGGGTCA TAATCATTTA 
ATTGGACAGC AGATTAGACA AGTGACACCT AATCCACAGT ATTCATCAAT GCAGCCAGCA 
CAGAATATAC CCCAGGGCTA CACCTCCTAC GGCTCACACA TGGGGATGCA GCCACACCCC 
TCTCAGACCC CTGGAATGGT TCCCAATTCA TATGGAAACC AGGGCTTTCA GACAGGACAT 
CCTGCCACTA ACCCAACTAT GGTGGACTCA ATAAGGCAGA TGCAGCAGAG ACCTGGCTAC 
GTCCATCAGC AGGCTCCTGC GGCATATGGG CACACAATCC AGAGCACACA AAGGTTTCCG 
CATCAATCCA TGCAGCAGAC ACCCATGATG CACGGCTTGG CTCAAAGTCA CTTGGGAGTG 
CAGGGCATCC ACCCAAACAT GAGGCCCAAT CAGATGATGG AGCAGCAGCA GCAACAACAA 
CAACAACAAC AGCAACAGCA GCAACAACAA CAACAACAAC AGGTCCACCA GCAGCAGCAG 
CACATCCGAC AGGGTCCAGT TTTTAGACCA CAGCAGCAAC AGCAGGTTCA GCAGCAAGTG 
CAACAGCAGG TCCAGCAGCA GCAGGTCCAA CAGCAGCAGG TCCAGCAACA GGTTCAGCCT 
CAGCAAGTTC AACAGCAGCA GGTTCAGCAG CAGCAGCAGC AGGTGTCTGC AGCTCAGCCT 
CCCGCTCAGG CCCTGGGCAT GCAGGCTCTG CCCCCGCAAC AGCCCATGTT CCAGCGGCAA 
GGCATGCAGC AAACCCAGCA GCAGCAGCAG ACCGCAGCAC TGGTCAGGCA ACTCCAGCAG 
CAGCTCTCCA GTACTCAACC TGCACAGAAC AACAGCTCCT ATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_009291037.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289312.1 Danio rerio mediator complex subunit 12 (med12), transcript variant X1, mRNA.

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CloneID	ODa44929
Clone ID Related Accession (Same CDS sequence)	XM_009291037.2 , XM_009291037.3
Accession Version	XM_009291037.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGCAGATGTT TTCTCCTCCA ATGCACTGTG AGTCGAAGGG AAGCCCTTCT 
CCAGAGAAAC AAGCCCAAGA GCAGGAGAGC AAAAGCACTG CCAAGGATAA GGGCATGGAC 
CCAGCCTTCC CTTTGGTTTA TGAACAGCCC CGCCACATAC AGTACGCCAC CCACTTCCCC 
ATTCCTCAGG AGGAGAGTGC AAGTCATGAG TGTAACCAGC GTCTAGTGGT TCTCTATGGA 
GTCGGCAAGC AGCGAGATGA AGCTCGCCAC GCCATCAAGA AGATCACCAA AGACATCCTG 
AAGGTTCTCA ACCGCAAGAG TACAGCAGAG ACAGGAGGTG AGGAAGGCCA GAAGAGGAAG 
AGGAGCAAAC CTGAGGCCTT CCCCACTGCT GAAGATATTT TCTCTAAATT CCAGCACCTC 
TCACACTTTG ACCAGCACCA GGTCACCTCA CAGGTGTCCA GGAATGTTCT GGAGCAAATC 
ACCAGCTTTG CCTTAGGGAT GTCCTATCAC CTCCCACTGG TCCAACACAT CCAGTTCATC 
TTTGACCTCA TGGAGTACTC TCTTAATATA AGTGGCCTCA TTGACTTTGC TATACAGCTG 
CTGAATGAAT TGAGTCTGGT GGAAGCAGAA CTCCTGCTTA AGTCCTCCAA CCTGGCGGGC 
AGTTACACTA CAGGCCTCTG TTTGTGTATC GTGGCTGTTC TGCGGAGATA CCACTCCTGC 
CTCATCCTCA ACCCTGACCA GACCGCACAG GTCTTTGACG GGTTGCGGAT TGTAGTCAAG 
TCTGGTGTGA ATCCTGCAGA CTGTTCCTCA GCAGAACGCT GTATCCTAGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTAAAG AGCAAGTTTG GAGAGATTTT CAGTGAGTTC 
TGCTCAAAGG TGAAGAATTC CATCTATTAT AACATCGACC CGTCAGACTC CAACATGCTG 
TGGGATCAGA TGTTTATGAT CGATGCCATC ACTAATCCTA CCGCACACAA CCTCAACCAC 
TCCATGTTGG GAAAGATCAC CAATGAACTC AATGACAGCC CGGCTAACCG CTACAGCTTT 
GTGTGTAATG TGCTCATGGA TGTTTGCGTT GACCATCGAG ATCCTGAGAG GGTGAATGAT 
ATTGGTATCC TGTGTGCGGA GCTGACAGCA TACTGTCGCT CTCTAAGTGC TGAGTGGTTG 
GGTGTGCTCA AGGCCCTCTG CTGCTCTTCT AACAACGGCA ACTGTGGCTT CAACGATCTG 
CTCTGCAATG TTGACGTGAG CGATCTATCT TTCCACGACT CTCTAGCAAC ATTCGTTGCC 
ATCTTAATAG CTCGGCAGTG TTTGTTGCTG GAAGATCTGG TGCGCTGTGT TGCCATCCCA 
TCCCTCCTGA ATGCAGCCTG TAGTGAACAG GATTCTGAAC CTGGAGCCAG ACTGACATGT 
AGGATCCTGC TTCATCTGTT CAGAACCCCA CAGCGCAACC CCTGTCCCCA GGATGGCACT 
AAATCAGACA AATCCATAGT GGGGATCAGG TCGTCCTGCG ACCGACATCT GCTGGCTGCT 
TCACAGAACA GCATCGTGGT GGGAGCCGTT TTTGCTGTAT TAAAAGCTGT GTTCATGCTG 
GGAGATGCTG AGCTGAAAGG CTCAGGCTTT TCCCATCCTG CTGGTCTGGA TGATATTGGA 
GAGGATGACA TGGGCTCTAA AAAATCTGGA GGACGTAATG TCTCTATTGA AACAGCCAGT 
CTGGTGGTTT ACGCCAAGTA TGTGCTGAAG AGCATCTGCC ACCAGGAATG GGTTGGCGAG 
CGCTGTCTGA AGTCTCTGTC AGAAGACAGC AGTGCACTGC AGGATCCAGT GCTGGTCAAC 
ATCCAGGCTC AGCGCTTGCT GCAGCTCATC TGTTACCCAC ACCGACAGCT GGACAGTGAA 
GAGGGTGACA ATCCACAGAG ACAGCGCATC AAACGCATCC TGCAGAACAT GGACCAGTGG 
ACCATGAGAC AGTCCTCTCT TGAGCTACAA CTCATGATCA AGCAAAGCTC AAATAATGAG 
CTGTATTCTC TTCTGGAGAA CATAGCCAAG GCCACTATTG AGGTTTTCCA GAAGTCTGCA 
GAGATGAACT CTAGTAACCC CTCCTGGAAT GGCTCTGCTG TCTCGGGTAG CTCAGTCTCC 
AATAGCAACA GTGCCAGCAA ACTCAAACCT GTGCTAAGTT CCTCTGAGCG TTCAGGTGTC 
TGGTTAGTTG CCCCTCTAAT TGGTAAGCTG CCCACCTCAG TGCAGGGGCA CGTGCTCAAG 
GCTGCAGGCG AGGAGCTGGA GAAGGGACAG CATCTGGGAC CCTCTTCACG AAAAGAAAGA 
GATCGACAGA AGCAAAAAAG CATGTCTCTC CTGAGCCAGC AGCCCTTCCT GTCACTGGTT 
CTGACATGTC TCAAAGGGCA AGACGAGCAG AGAGAGGGTC TCTTGACGTC CCTCTACAGC 
CAAGTCCAGC AGATTGTGAC TAACTGGCGA GAGGACCAGT ACCAGGACGA CTGCAAGGCT 
AAGCAGATGA TGCACGAGGC CTTGAAGCTG CGACTCAATC TGGTGGGTGG GATGTTTGAC 
ACTGTACAGC GTAGCACTCA GCAGACAACA GAGTGGGCAG TGCTTCTGCT GGACATCATC 
AGCAGTGGCA CAGTGGACAT GCAGTCTAAC AATGAGCTCT TCACTACGGT TTTAGACATG 
CTGAGCGTGT TGATTAACGG GACTCTGGCG GCTGACATGT CCAGCATATC TCAGGGCAGC 
ATGGAGGAGA ATAAGAGAGC TTACATGAAC CTGGTCAAGA AGCTCAGGAA AGAACTTGGT 
GACCGACAAT CCGAGAGCTT GGAGAAAGTG CGTCAGTTGC TGCCTCTGCC CAAGCAGACG 
CGTGATGTCA TCACCTGTGA GCCCCAGGGG TCACTCATTG ACACAAAGGG CAACAAAATT 
GCTGGATTTG AAAAAGAGGG TCTTCAGGTA TCAACTAAGC AGAAGATTTC TCCATGGGAT 
GTGTTTGAGG GACTAAAGCA CTCTGCTCCG CTCTCCTGGG GCTGGTTCGG AACCATACGG 
GTCGACCGCA AAGTCACTAA GTTTGAAGAG CAGCAGCGGC TCCTGCTTTA CCACACACAC 
ATCAAGCCCA AGCAACGCAG TTACTACCTG GCGCCCCTCA ACCTGCCCCA AGAAGAAGAG 
GAACCACTCA CTCCTGTGCC TCCCGAGCCA GACAAGAAGC TGGACACAGC GAAGGTGGAG 
AAAAGCGTCT CCAATGTCCC GACTAAGAAA AAGAAAAAGA AACCTGCATC GACGGTTAAA 
CAAGAGGACT ACAAGCCACA CAACACAGTC ATGCAGTACG CTCCAGGCAT GACCCCTGAT 
CTCCCACTGA GCATGGGACA AACAAACATG TCATATCGAA TGGGCTACCA AGCTCCAATG 
AACATGTATG CCCAGAACCA GCCTTTACCA CCAGGAGGTC CTGGTTTGGA GCCACCTTTT 
CGTCCTAGGG TTCCTATGAA TAAAATGCCT CCACGTCCCA ACTACACTAT GCCTAATATG 
CAGACCGCTG GCATGGGCAA CCTAATGGGA ATGGAGAAGC AGTACCAGAT GAACTATAAA 
CCCATGCCCA ACATGGCTCA GGGGCAGATG CTCCGTCATC AGCTACAGGT CAGACTGGGT 
CATAATCATT TAATTGGACA GCAGATTAGA CAAGTGACAC CTAATCCACA GTATTCATCA 
ATGCAGCCAG CACAGAATAT ACCCCAGGGC TACACCTCCT ACGGCTCACA CATGGGGATG 
CAGCCACACC CCTCTCAGAC CCCTGGAATG GTTCCCAATT CATATGGAAA CCAGGGCTTT 
CAGACAGGAC ATCCTGCCAC TAACCCAACT ATGGTGGACT CAATAAGGCA GATGCAGCAG 
AGACCTGGCT ACGTCCATCA GCAGGCTCCT GCGGCATATG GGCACACAAT CCAGAGCACA 
CAAAGGTTTC CGCATCAATC CATGCAGCAG ACACCCATGA TGCACGGCTT GGCTCAAAGT 
CACTTGGGAG TGCAGGGCAT CCACCCAAAC ATGAGGCCCA ATCAGATGAT GGAGCAGCAG 
CAGCAACAAC AACAACAACA ACAGCAACAG CAGCAACAAC AACAACAACA ACAGGTCCAC 
CAGCAGCAGC AGCACATCCG ACAGGGTCCA GTTTTTAGAC CACAGCAGCA ACAGCAGGTT 
CAGCAGCAAG TGCAACAGCA GGTCCAGCAG CAGCAGGTCC AACAGCAGCA GGTCCAGCAA 
CAGGTTCAGC CTCAGCAAGT TCAACAGCAG CAGGTTCAGC AGCAGCAGCA GCAGGTGTCT 
GCAGCTCAGC CTCCCGCTCA GGCCCTGGGC ATGCAGGCTC TGCCCCCGCA ACAGCCCATG 
TTCCAGCGGC AAGGCATGCA GCAAACCCAG CAGCAGCAGC AGACCGCAGC ACTGGTCAGG 
CAACTCCAGC AGCAGCTCTC CAGTACTCAA CCTGCACAGA ACAACAGCTC CTATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289312.1
CDS	92..6631
Translation	MMAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNSSKISSNFSSIIAEKLRCNTFPDTGKRKPQVNQKDNFWLVTARSQSSINNWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYSVPVMRSAWMIKMTCAYHAAITENKMKKRHVIDPCIEWTQIITKYLWEQLQKVAEFYRQSPSQGCGSPLPAPPAEVETAMKQWEYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLRLLLPLLLQYSGEFVQSAYLSRRLAYFCTRRLNLLLSDGSIGPGPGGHQAHGISAQQGNALPPTPTSQPAGGNQPQTPFTDFYICPQHRPVVFGLSCMLQSIVLCCPSALVWHYSLTDSRNKTGSPLDLLPIAPSNLPMPGGNSTFNQQVRAKVREIEEQIKERGQAVEFRWSFDKCQETTAGFTISRVLHTLEVLDNHSFEKSDFSNSLDSLYNRIFGSGQSKDGHEMSPDDDAVVTLLCEWAVSCKRSGPHRAMVVAKLLEKRQTEIEAERCGESEVVDEKGSVSSGSLSAATLPVFQDVLLQFLDTQAPVLTEPGNESERVEFSNLVLLFYELIRHDVFSHNIYMCTLISRGDLASNSHLPRPRSPSDEPSDESERKDQDAGSSVKIEDTGMSESMEIDHNSSANFDEQMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESASHECNQRLVVLYGVGKQRDEARHAIKKITKDILKVLNRKSTAETGGEEGQKRKRSKPEAFPTAEDIFSKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSLVEAELLLKSSNLAGSYTTGLCLCIVAVLRRYHSCLILNPDQTAQVFDGLRIVVKSGVNPADCSSAERCILAYLYDLYTSCSHLKSKFGEIFSEFCSKVKNSIYYNIDPSDSNMLWDQMFMIDAITNPTAHNLNHSMLGKITNELNDSPANRYSFVCNVLMDVCVDHRDPERVNDIGILCAELTAYCRSLSAEWLGVLKALCCSSNNGNCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLVRCVAIPSLLNAACSEQDSEPGARLTCRILLHLFRTPQRNPCPQDGTKSDKSIVGIRSSCDRHLLAASQNSIVVGAVFAVLKAVFMLGDAELKGSGFSHPAGLDDIGEDDMGSKKSGGRNVSIETASLVVYAKYVLKSICHQEWVGERCLKSLSEDSSALQDPVLVNIQAQRLLQLICYPHRQLDSEEGDNPQRQRIKRILQNMDQWTMRQSSLELQLMIKQSSNNELYSLLENIAKATIEVFQKSAEMNSSNPSWNGSAVSGSSVSNSNSASKLKPVLSSSERSGVWLVAPLIGKLPTSVQGHVLKAAGEELEKGQHLGPSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQMMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFEKEGLQVSTKQKISPWDVFEGLKHSAPLSWGWFGTIRVDRKVTKFEEQQRLLLYHTHIKPKQRSYYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMSYRMGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQYQMNYKPMPNMAQGQMLRHQLQVRLGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQQQQQVQQQVQQQVQQQQVQQQQVQQQVQPQQVQQQQVQQQQQQVSAAQPPAQALGMQALPPQQPMFQRQGMQQTQQQQQTAALVRQLQQQLSSTQPAQNNSSYY

Target ORF information:

RefSeq Version	XM_009291037.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 12 (med12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291037.2

ORF Insert Sequence:

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGCAGATGTT TTCTCCTCCA ATGCACTGTG AGTCGAAGGG AAGCCCTTCT 
CCAGAGAAAC AAGCCCAAGA GCAGGAGAGC AAAAGCACTG CCAAGGATAA GGGCATGGAC 
CCAGCCTTCC CTTTGGTTTA TGAACAGCCC CGCCACATAC AGTACGCCAC CCACTTCCCC 
ATTCCTCAGG AGGAGAGTGC AAGTCATGAG TGTAACCAGC GTCTAGTGGT TCTCTATGGA 
GTCGGCAAGC AGCGAGATGA AGCTCGCCAC GCCATCAAGA AGATCACCAA AGACATCCTG 
AAGGTTCTCA ACCGCAAGAG TACAGCAGAG ACAGGAGGTG AGGAAGGCCA GAAGAGGAAG 
AGGAGCAAAC CTGAGGCCTT CCCCACTGCT GAAGATATTT TCTCTAAATT CCAGCACCTC 
TCACACTTTG ACCAGCACCA GGTCACCTCA CAGGTGTCCA GGAATGTTCT GGAGCAAATC 
ACCAGCTTTG CCTTAGGGAT GTCCTATCAC CTCCCACTGG TCCAACACAT CCAGTTCATC 
TTTGACCTCA TGGAGTACTC TCTTAATATA AGTGGCCTCA TTGACTTTGC TATACAGCTG 
CTGAATGAAT TGAGTCTGGT GGAAGCAGAA CTCCTGCTTA AGTCCTCCAA CCTGGCGGGC 
AGTTACACTA CAGGCCTCTG TTTGTGTATC GTGGCTGTTC TGCGGAGATA CCACTCCTGC 
CTCATCCTCA ACCCTGACCA GACCGCACAG GTCTTTGACG GGTTGCGGAT TGTAGTCAAG 
TCTGGTGTGA ATCCTGCAGA CTGTTCCTCA GCAGAACGCT GTATCCTAGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTAAAG AGCAAGTTTG GAGAGATTTT CAGTGAGTTC 
TGCTCAAAGG TGAAGAATTC CATCTATTAT AACATCGACC CGTCAGACTC CAACATGCTG 
TGGGATCAGA TGTTTATGAT CGATGCCATC ACTAATCCTA CCGCACACAA CCTCAACCAC 
TCCATGTTGG GAAAGATCAC CAATGAACTC AATGACAGCC CGGCTAACCG CTACAGCTTT 
GTGTGTAATG TGCTCATGGA TGTTTGCGTT GACCATCGAG ATCCTGAGAG GGTGAATGAT 
ATTGGTATCC TGTGTGCGGA GCTGACAGCA TACTGTCGCT CTCTAAGTGC TGAGTGGTTG 
GGTGTGCTCA AGGCCCTCTG CTGCTCTTCT AACAACGGCA ACTGTGGCTT CAACGATCTG 
CTCTGCAATG TTGACGTGAG CGATCTATCT TTCCACGACT CTCTAGCAAC ATTCGTTGCC 
ATCTTAATAG CTCGGCAGTG TTTGTTGCTG GAAGATCTGG TGCGCTGTGT TGCCATCCCA 
TCCCTCCTGA ATGCAGCCTG TAGTGAACAG GATTCTGAAC CTGGAGCCAG ACTGACATGT 
AGGATCCTGC TTCATCTGTT CAGAACCCCA CAGCGCAACC CCTGTCCCCA GGATGGCACT 
AAATCAGACA AATCCATAGT GGGGATCAGG TCGTCCTGCG ACCGACATCT GCTGGCTGCT 
TCACAGAACA GCATCGTGGT GGGAGCCGTT TTTGCTGTAT TAAAAGCTGT GTTCATGCTG 
GGAGATGCTG AGCTGAAAGG CTCAGGCTTT TCCCATCCTG CTGGTCTGGA TGATATTGGA 
GAGGATGACA TGGGCTCTAA AAAATCTGGA GGACGTAATG TCTCTATTGA AACAGCCAGT 
CTGGTGGTTT ACGCCAAGTA TGTGCTGAAG AGCATCTGCC ACCAGGAATG GGTTGGCGAG 
CGCTGTCTGA AGTCTCTGTC AGAAGACAGC AGTGCACTGC AGGATCCAGT GCTGGTCAAC 
ATCCAGGCTC AGCGCTTGCT GCAGCTCATC TGTTACCCAC ACCGACAGCT GGACAGTGAA 
GAGGGTGACA ATCCACAGAG ACAGCGCATC AAACGCATCC TGCAGAACAT GGACCAGTGG 
ACCATGAGAC AGTCCTCTCT TGAGCTACAA CTCATGATCA AGCAAAGCTC AAATAATGAG 
CTGTATTCTC TTCTGGAGAA CATAGCCAAG GCCACTATTG AGGTTTTCCA GAAGTCTGCA 
GAGATGAACT CTAGTAACCC CTCCTGGAAT GGCTCTGCTG TCTCGGGTAG CTCAGTCTCC 
AATAGCAACA GTGCCAGCAA ACTCAAACCT GTGCTAAGTT CCTCTGAGCG TTCAGGTGTC 
TGGTTAGTTG CCCCTCTAAT TGGTAAGCTG CCCACCTCAG TGCAGGGGCA CGTGCTCAAG 
GCTGCAGGCG AGGAGCTGGA GAAGGGACAG CATCTGGGAC CCTCTTCACG AAAAGAAAGA 
GATCGACAGA AGCAAAAAAG CATGTCTCTC CTGAGCCAGC AGCCCTTCCT GTCACTGGTT 
CTGACATGTC TCAAAGGGCA AGACGAGCAG AGAGAGGGTC TCTTGACGTC CCTCTACAGC 
CAAGTCCAGC AGATTGTGAC TAACTGGCGA GAGGACCAGT ACCAGGACGA CTGCAAGGCT 
AAGCAGATGA TGCACGAGGC CTTGAAGCTG CGACTCAATC TGGTGGGTGG GATGTTTGAC 
ACTGTACAGC GTAGCACTCA GCAGACAACA GAGTGGGCAG TGCTTCTGCT GGACATCATC 
AGCAGTGGCA CAGTGGACAT GCAGTCTAAC AATGAGCTCT TCACTACGGT TTTAGACATG 
CTGAGCGTGT TGATTAACGG GACTCTGGCG GCTGACATGT CCAGCATATC TCAGGGCAGC 
ATGGAGGAGA ATAAGAGAGC TTACATGAAC CTGGTCAAGA AGCTCAGGAA AGAACTTGGT 
GACCGACAAT CCGAGAGCTT GGAGAAAGTG CGTCAGTTGC TGCCTCTGCC CAAGCAGACG 
CGTGATGTCA TCACCTGTGA GCCCCAGGGG TCACTCATTG ACACAAAGGG CAACAAAATT 
GCTGGATTTG AAAAAGAGGG TCTTCAGGTA TCAACTAAGC AGAAGATTTC TCCATGGGAT 
GTGTTTGAGG GACTAAAGCA CTCTGCTCCG CTCTCCTGGG GCTGGTTCGG AACCATACGG 
GTCGACCGCA AAGTCACTAA GTTTGAAGAG CAGCAGCGGC TCCTGCTTTA CCACACACAC 
ATCAAGCCCA AGCAACGCAG TTACTACCTG GCGCCCCTCA ACCTGCCCCA AGAAGAAGAG 
GAACCACTCA CTCCTGTGCC TCCCGAGCCA GACAAGAAGC TGGACACAGC GAAGGTGGAG 
AAAAGCGTCT CCAATGTCCC GACTAAGAAA AAGAAAAAGA AACCTGCATC GACGGTTAAA 
CAAGAGGACT ACAAGCCACA CAACACAGTC ATGCAGTACG CTCCAGGCAT GACCCCTGAT 
CTCCCACTGA GCATGGGACA AACAAACATG TCATATCGAA TGGGCTACCA AGCTCCAATG 
AACATGTATG CCCAGAACCA GCCTTTACCA CCAGGAGGTC CTGGTTTGGA GCCACCTTTT 
CGTCCTAGGG TTCCTATGAA TAAAATGCCT CCACGTCCCA ACTACACTAT GCCTAATATG 
CAGACCGCTG GCATGGGCAA CCTAATGGGA ATGGAGAAGC AGTACCAGAT GAACTATAAA 
CCCATGCCCA ACATGGCTCA GGGGCAGATG CTCCGTCATC AGCTACAGGT CAGACTGGGT 
CATAATCATT TAATTGGACA GCAGATTAGA CAAGTGACAC CTAATCCACA GTATTCATCA 
ATGCAGCCAG CACAGAATAT ACCCCAGGGC TACACCTCCT ACGGCTCACA CATGGGGATG 
CAGCCACACC CCTCTCAGAC CCCTGGAATG GTTCCCAATT CATATGGAAA CCAGGGCTTT 
CAGACAGGAC ATCCTGCCAC TAACCCAACT ATGGTGGACT CAATAAGGCA GATGCAGCAG 
AGACCTGGCT ACGTCCATCA GCAGGCTCCT GCGGCATATG GGCACACAAT CCAGAGCACA 
CAAAGGTTTC CGCATCAATC CATGCAGCAG ACACCCATGA TGCACGGCTT GGCTCAAAGT 
CACTTGGGAG TGCAGGGCAT CCACCCAAAC ATGAGGCCCA ATCAGATGAT GGAGCAGCAG 
CAGCAACAAC AACAACAACA ACAGCAACAG CAGCAACAAC AACAACAACA ACAGGTCCAC 
CAGCAGCAGC AGCACATCCG ACAGGGTCCA GTTTTTAGAC CACAGCAGCA ACAGCAGGTT 
CAGCAGCAAG TGCAACAGCA GGTCCAGCAG CAGCAGGTCC AACAGCAGCA GGTCCAGCAA 
CAGGTTCAGC CTCAGCAAGT TCAACAGCAG CAGGTTCAGC AGCAGCAGCA GCAGGTGTCT 
GCAGCTCAGC CTCCCGCTCA GGCCCTGGGC ATGCAGGCTC TGCCCCCGCA ACAGCCCATG 
TTCCAGCGGC AAGGCATGCA GCAAACCCAG CAGCAGCAGC AGACCGCAGC ACTGGTCAGG 
CAACTCCAGC AGCAGCTCTC CAGTACTCAA CCTGCACAGA ACAACAGCTC CTATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_009291037.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289312.1 Danio rerio mediator complex subunit 12 (med12), transcript variant X1, mRNA.

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CloneID	ODa44929
Clone ID Related Accession (Same CDS sequence)	XM_009291037.2 , XM_009291037.3
Accession Version	XM_009291037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6540bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGCAGATGTT TTCTCCTCCA ATGCACTGTG AGTCGAAGGG AAGCCCTTCT 
CCAGAGAAAC AAGCCCAAGA GCAGGAGAGC AAAAGCACTG CCAAGGATAA GGGCATGGAC 
CCAGCCTTCC CTTTGGTTTA TGAACAGCCC CGCCACATAC AGTACGCCAC CCACTTCCCC 
ATTCCTCAGG AGGAGAGTGC AAGTCATGAG TGTAACCAGC GTCTAGTGGT TCTCTATGGA 
GTCGGCAAGC AGCGAGATGA AGCTCGCCAC GCCATCAAGA AGATCACCAA AGACATCCTG 
AAGGTTCTCA ACCGCAAGAG TACAGCAGAG ACAGGAGGTG AGGAAGGCCA GAAGAGGAAG 
AGGAGCAAAC CTGAGGCCTT CCCCACTGCT GAAGATATTT TCTCTAAATT CCAGCACCTC 
TCACACTTTG ACCAGCACCA GGTCACCTCA CAGGTGTCCA GGAATGTTCT GGAGCAAATC 
ACCAGCTTTG CCTTAGGGAT GTCCTATCAC CTCCCACTGG TCCAACACAT CCAGTTCATC 
TTTGACCTCA TGGAGTACTC TCTTAATATA AGTGGCCTCA TTGACTTTGC TATACAGCTG 
CTGAATGAAT TGAGTCTGGT GGAAGCAGAA CTCCTGCTTA AGTCCTCCAA CCTGGCGGGC 
AGTTACACTA CAGGCCTCTG TTTGTGTATC GTGGCTGTTC TGCGGAGATA CCACTCCTGC 
CTCATCCTCA ACCCTGACCA GACCGCACAG GTCTTTGACG GGTTGCGGAT TGTAGTCAAG 
TCTGGTGTGA ATCCTGCAGA CTGTTCCTCA GCAGAACGCT GTATCCTAGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTAAAG AGCAAGTTTG GAGAGATTTT CAGTGAGTTC 
TGCTCAAAGG TGAAGAATTC CATCTATTAT AACATCGACC CGTCAGACTC CAACATGCTG 
TGGGATCAGA TGTTTATGAT CGATGCCATC ACTAATCCTA CCGCACACAA CCTCAACCAC 
TCCATGTTGG GAAAGATCAC CAATGAACTC AATGACAGCC CGGCTAACCG CTACAGCTTT 
GTGTGTAATG TGCTCATGGA TGTTTGCGTT GACCATCGAG ATCCTGAGAG GGTGAATGAT 
ATTGGTATCC TGTGTGCGGA GCTGACAGCA TACTGTCGCT CTCTAAGTGC TGAGTGGTTG 
GGTGTGCTCA AGGCCCTCTG CTGCTCTTCT AACAACGGCA ACTGTGGCTT CAACGATCTG 
CTCTGCAATG TTGACGTGAG CGATCTATCT TTCCACGACT CTCTAGCAAC ATTCGTTGCC 
ATCTTAATAG CTCGGCAGTG TTTGTTGCTG GAAGATCTGG TGCGCTGTGT TGCCATCCCA 
TCCCTCCTGA ATGCAGCCTG TAGTGAACAG GATTCTGAAC CTGGAGCCAG ACTGACATGT 
AGGATCCTGC TTCATCTGTT CAGAACCCCA CAGCGCAACC CCTGTCCCCA GGATGGCACT 
AAATCAGACA AATCCATAGT GGGGATCAGG TCGTCCTGCG ACCGACATCT GCTGGCTGCT 
TCACAGAACA GCATCGTGGT GGGAGCCGTT TTTGCTGTAT TAAAAGCTGT GTTCATGCTG 
GGAGATGCTG AGCTGAAAGG CTCAGGCTTT TCCCATCCTG CTGGTCTGGA TGATATTGGA 
GAGGATGACA TGGGCTCTAA AAAATCTGGA GGACGTAATG TCTCTATTGA AACAGCCAGT 
CTGGTGGTTT ACGCCAAGTA TGTGCTGAAG AGCATCTGCC ACCAGGAATG GGTTGGCGAG 
CGCTGTCTGA AGTCTCTGTC AGAAGACAGC AGTGCACTGC AGGATCCAGT GCTGGTCAAC 
ATCCAGGCTC AGCGCTTGCT GCAGCTCATC TGTTACCCAC ACCGACAGCT GGACAGTGAA 
GAGGGTGACA ATCCACAGAG ACAGCGCATC AAACGCATCC TGCAGAACAT GGACCAGTGG 
ACCATGAGAC AGTCCTCTCT TGAGCTACAA CTCATGATCA AGCAAAGCTC AAATAATGAG 
CTGTATTCTC TTCTGGAGAA CATAGCCAAG GCCACTATTG AGGTTTTCCA GAAGTCTGCA 
GAGATGAACT CTAGTAACCC CTCCTGGAAT GGCTCTGCTG TCTCGGGTAG CTCAGTCTCC 
AATAGCAACA GTGCCAGCAA ACTCAAACCT GTGCTAAGTT CCTCTGAGCG TTCAGGTGTC 
TGGTTAGTTG CCCCTCTAAT TGGTAAGCTG CCCACCTCAG TGCAGGGGCA CGTGCTCAAG 
GCTGCAGGCG AGGAGCTGGA GAAGGGACAG CATCTGGGAC CCTCTTCACG AAAAGAAAGA 
GATCGACAGA AGCAAAAAAG CATGTCTCTC CTGAGCCAGC AGCCCTTCCT GTCACTGGTT 
CTGACATGTC TCAAAGGGCA AGACGAGCAG AGAGAGGGTC TCTTGACGTC CCTCTACAGC 
CAAGTCCAGC AGATTGTGAC TAACTGGCGA GAGGACCAGT ACCAGGACGA CTGCAAGGCT 
AAGCAGATGA TGCACGAGGC CTTGAAGCTG CGACTCAATC TGGTGGGTGG GATGTTTGAC 
ACTGTACAGC GTAGCACTCA GCAGACAACA GAGTGGGCAG TGCTTCTGCT GGACATCATC 
AGCAGTGGCA CAGTGGACAT GCAGTCTAAC AATGAGCTCT TCACTACGGT TTTAGACATG 
CTGAGCGTGT TGATTAACGG GACTCTGGCG GCTGACATGT CCAGCATATC TCAGGGCAGC 
ATGGAGGAGA ATAAGAGAGC TTACATGAAC CTGGTCAAGA AGCTCAGGAA AGAACTTGGT 
GACCGACAAT CCGAGAGCTT GGAGAAAGTG CGTCAGTTGC TGCCTCTGCC CAAGCAGACG 
CGTGATGTCA TCACCTGTGA GCCCCAGGGG TCACTCATTG ACACAAAGGG CAACAAAATT 
GCTGGATTTG AAAAAGAGGG TCTTCAGGTA TCAACTAAGC AGAAGATTTC TCCATGGGAT 
GTGTTTGAGG GACTAAAGCA CTCTGCTCCG CTCTCCTGGG GCTGGTTCGG AACCATACGG 
GTCGACCGCA AAGTCACTAA GTTTGAAGAG CAGCAGCGGC TCCTGCTTTA CCACACACAC 
ATCAAGCCCA AGCAACGCAG TTACTACCTG GCGCCCCTCA ACCTGCCCCA AGAAGAAGAG 
GAACCACTCA CTCCTGTGCC TCCCGAGCCA GACAAGAAGC TGGACACAGC GAAGGTGGAG 
AAAAGCGTCT CCAATGTCCC GACTAAGAAA AAGAAAAAGA AACCTGCATC GACGGTTAAA 
CAAGAGGACT ACAAGCCACA CAACACAGTC ATGCAGTACG CTCCAGGCAT GACCCCTGAT 
CTCCCACTGA GCATGGGACA AACAAACATG TCATATCGAA TGGGCTACCA AGCTCCAATG 
AACATGTATG CCCAGAACCA GCCTTTACCA CCAGGAGGTC CTGGTTTGGA GCCACCTTTT 
CGTCCTAGGG TTCCTATGAA TAAAATGCCT CCACGTCCCA ACTACACTAT GCCTAATATG 
CAGACCGCTG GCATGGGCAA CCTAATGGGA ATGGAGAAGC AGTACCAGAT GAACTATAAA 
CCCATGCCCA ACATGGCTCA GGGGCAGATG CTCCGTCATC AGCTACAGGT CAGACTGGGT 
CATAATCATT TAATTGGACA GCAGATTAGA CAAGTGACAC CTAATCCACA GTATTCATCA 
ATGCAGCCAG CACAGAATAT ACCCCAGGGC TACACCTCCT ACGGCTCACA CATGGGGATG 
CAGCCACACC CCTCTCAGAC CCCTGGAATG GTTCCCAATT CATATGGAAA CCAGGGCTTT 
CAGACAGGAC ATCCTGCCAC TAACCCAACT ATGGTGGACT CAATAAGGCA GATGCAGCAG 
AGACCTGGCT ACGTCCATCA GCAGGCTCCT GCGGCATATG GGCACACAAT CCAGAGCACA 
CAAAGGTTTC CGCATCAATC CATGCAGCAG ACACCCATGA TGCACGGCTT GGCTCAAAGT 
CACTTGGGAG TGCAGGGCAT CCACCCAAAC ATGAGGCCCA ATCAGATGAT GGAGCAGCAG 
CAGCAACAAC AACAACAACA ACAGCAACAG CAGCAACAAC AACAACAACA ACAGGTCCAC 
CAGCAGCAGC AGCACATCCG ACAGGGTCCA GTTTTTAGAC CACAGCAGCA ACAGCAGGTT 
CAGCAGCAAG TGCAACAGCA GGTCCAGCAG CAGCAGGTCC AACAGCAGCA GGTCCAGCAA 
CAGGTTCAGC CTCAGCAAGT TCAACAGCAG CAGGTTCAGC AGCAGCAGCA GCAGGTGTCT 
GCAGCTCAGC CTCCCGCTCA GGCCCTGGGC ATGCAGGCTC TGCCCCCGCA ACAGCCCATG 
TTCCAGCGGC AAGGCATGCA GCAAACCCAG CAGCAGCAGC AGACCGCAGC ACTGGTCAGG 
CAACTCCAGC AGCAGCTCTC CAGTACTCAA CCTGCACAGA ACAACAGCTC CTATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289312.1
CDS	95..6634
Translation	MMAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNSSKISSNFSSIIAEKLRCNTFPDTGKRKPQVNQKDNFWLVTARSQSSINNWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYSVPVMRSAWMIKMTCAYHAAITENKMKKRHVIDPCIEWTQIITKYLWEQLQKVAEFYRQSPSQGCGSPLPAPPAEVETAMKQWEYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLRLLLPLLLQYSGEFVQSAYLSRRLAYFCTRRLNLLLSDGSIGPGPGGHQAHGISAQQGNALPPTPTSQPAGGNQPQTPFTDFYICPQHRPVVFGLSCMLQSIVLCCPSALVWHYSLTDSRNKTGSPLDLLPIAPSNLPMPGGNSTFNQQVRAKVREIEEQIKERGQAVEFRWSFDKCQETTAGFTISRVLHTLEVLDNHSFEKSDFSNSLDSLYNRIFGSGQSKDGHEMSPDDDAVVTLLCEWAVSCKRSGPHRAMVVAKLLEKRQTEIEAERCGESEVVDEKGSVSSGSLSAATLPVFQDVLLQFLDTQAPVLTEPGNESERVEFSNLVLLFYELIRHDVFSHNIYMCTLISRGDLASNSHLPRPRSPSDEPSDESERKDQDAGSSVKIEDTGMSESMEIDHNSSANFDEQMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESASHECNQRLVVLYGVGKQRDEARHAIKKITKDILKVLNRKSTAETGGEEGQKRKRSKPEAFPTAEDIFSKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSLVEAELLLKSSNLAGSYTTGLCLCIVAVLRRYHSCLILNPDQTAQVFDGLRIVVKSGVNPADCSSAERCILAYLYDLYTSCSHLKSKFGEIFSEFCSKVKNSIYYNIDPSDSNMLWDQMFMIDAITNPTAHNLNHSMLGKITNELNDSPANRYSFVCNVLMDVCVDHRDPERVNDIGILCAELTAYCRSLSAEWLGVLKALCCSSNNGNCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLVRCVAIPSLLNAACSEQDSEPGARLTCRILLHLFRTPQRNPCPQDGTKSDKSIVGIRSSCDRHLLAASQNSIVVGAVFAVLKAVFMLGDAELKGSGFSHPAGLDDIGEDDMGSKKSGGRNVSIETASLVVYAKYVLKSICHQEWVGERCLKSLSEDSSALQDPVLVNIQAQRLLQLICYPHRQLDSEEGDNPQRQRIKRILQNMDQWTMRQSSLELQLMIKQSSNNELYSLLENIAKATIEVFQKSAEMNSSNPSWNGSAVSGSSVSNSNSASKLKPVLSSSERSGVWLVAPLIGKLPTSVQGHVLKAAGEELEKGQHLGPSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQMMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFEKEGLQVSTKQKISPWDVFEGLKHSAPLSWGWFGTIRVDRKVTKFEEQQRLLLYHTHIKPKQRSYYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMSYRMGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQYQMNYKPMPNMAQGQMLRHQLQVRLGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQQQQQVQQQVQQQVQQQQVQQQQVQQQVQPQQVQQQQVQQQQQQVSAAQPPAQALGMQALPPQQPMFQRQGMQQTQQQQQTAALVRQLQQQLSSTQPAQNNSSYY

Target ORF information:

RefSeq Version	XM_009291037.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 12 (med12), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291037.3

ORF Insert Sequence:

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGCAGATGTT TTCTCCTCCA ATGCACTGTG AGTCGAAGGG AAGCCCTTCT 
CCAGAGAAAC AAGCCCAAGA GCAGGAGAGC AAAAGCACTG CCAAGGATAA GGGCATGGAC 
CCAGCCTTCC CTTTGGTTTA TGAACAGCCC CGCCACATAC AGTACGCCAC CCACTTCCCC 
ATTCCTCAGG AGGAGAGTGC AAGTCATGAG TGTAACCAGC GTCTAGTGGT TCTCTATGGA 
GTCGGCAAGC AGCGAGATGA AGCTCGCCAC GCCATCAAGA AGATCACCAA AGACATCCTG 
AAGGTTCTCA ACCGCAAGAG TACAGCAGAG ACAGGAGGTG AGGAAGGCCA GAAGAGGAAG 
AGGAGCAAAC CTGAGGCCTT CCCCACTGCT GAAGATATTT TCTCTAAATT CCAGCACCTC 
TCACACTTTG ACCAGCACCA GGTCACCTCA CAGGTGTCCA GGAATGTTCT GGAGCAAATC 
ACCAGCTTTG CCTTAGGGAT GTCCTATCAC CTCCCACTGG TCCAACACAT CCAGTTCATC 
TTTGACCTCA TGGAGTACTC TCTTAATATA AGTGGCCTCA TTGACTTTGC TATACAGCTG 
CTGAATGAAT TGAGTCTGGT GGAAGCAGAA CTCCTGCTTA AGTCCTCCAA CCTGGCGGGC 
AGTTACACTA CAGGCCTCTG TTTGTGTATC GTGGCTGTTC TGCGGAGATA CCACTCCTGC 
CTCATCCTCA ACCCTGACCA GACCGCACAG GTCTTTGACG GGTTGCGGAT TGTAGTCAAG 
TCTGGTGTGA ATCCTGCAGA CTGTTCCTCA GCAGAACGCT GTATCCTAGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTAAAG AGCAAGTTTG GAGAGATTTT CAGTGAGTTC 
TGCTCAAAGG TGAAGAATTC CATCTATTAT AACATCGACC CGTCAGACTC CAACATGCTG 
TGGGATCAGA TGTTTATGAT CGATGCCATC ACTAATCCTA CCGCACACAA CCTCAACCAC 
TCCATGTTGG GAAAGATCAC CAATGAACTC AATGACAGCC CGGCTAACCG CTACAGCTTT 
GTGTGTAATG TGCTCATGGA TGTTTGCGTT GACCATCGAG ATCCTGAGAG GGTGAATGAT 
ATTGGTATCC TGTGTGCGGA GCTGACAGCA TACTGTCGCT CTCTAAGTGC TGAGTGGTTG 
GGTGTGCTCA AGGCCCTCTG CTGCTCTTCT AACAACGGCA ACTGTGGCTT CAACGATCTG 
CTCTGCAATG TTGACGTGAG CGATCTATCT TTCCACGACT CTCTAGCAAC ATTCGTTGCC 
ATCTTAATAG CTCGGCAGTG TTTGTTGCTG GAAGATCTGG TGCGCTGTGT TGCCATCCCA 
TCCCTCCTGA ATGCAGCCTG TAGTGAACAG GATTCTGAAC CTGGAGCCAG ACTGACATGT 
AGGATCCTGC TTCATCTGTT CAGAACCCCA CAGCGCAACC CCTGTCCCCA GGATGGCACT 
AAATCAGACA AATCCATAGT GGGGATCAGG TCGTCCTGCG ACCGACATCT GCTGGCTGCT 
TCACAGAACA GCATCGTGGT GGGAGCCGTT TTTGCTGTAT TAAAAGCTGT GTTCATGCTG 
GGAGATGCTG AGCTGAAAGG CTCAGGCTTT TCCCATCCTG CTGGTCTGGA TGATATTGGA 
GAGGATGACA TGGGCTCTAA AAAATCTGGA GGACGTAATG TCTCTATTGA AACAGCCAGT 
CTGGTGGTTT ACGCCAAGTA TGTGCTGAAG AGCATCTGCC ACCAGGAATG GGTTGGCGAG 
CGCTGTCTGA AGTCTCTGTC AGAAGACAGC AGTGCACTGC AGGATCCAGT GCTGGTCAAC 
ATCCAGGCTC AGCGCTTGCT GCAGCTCATC TGTTACCCAC ACCGACAGCT GGACAGTGAA 
GAGGGTGACA ATCCACAGAG ACAGCGCATC AAACGCATCC TGCAGAACAT GGACCAGTGG 
ACCATGAGAC AGTCCTCTCT TGAGCTACAA CTCATGATCA AGCAAAGCTC AAATAATGAG 
CTGTATTCTC TTCTGGAGAA CATAGCCAAG GCCACTATTG AGGTTTTCCA GAAGTCTGCA 
GAGATGAACT CTAGTAACCC CTCCTGGAAT GGCTCTGCTG TCTCGGGTAG CTCAGTCTCC 
AATAGCAACA GTGCCAGCAA ACTCAAACCT GTGCTAAGTT CCTCTGAGCG TTCAGGTGTC 
TGGTTAGTTG CCCCTCTAAT TGGTAAGCTG CCCACCTCAG TGCAGGGGCA CGTGCTCAAG 
GCTGCAGGCG AGGAGCTGGA GAAGGGACAG CATCTGGGAC CCTCTTCACG AAAAGAAAGA 
GATCGACAGA AGCAAAAAAG CATGTCTCTC CTGAGCCAGC AGCCCTTCCT GTCACTGGTT 
CTGACATGTC TCAAAGGGCA AGACGAGCAG AGAGAGGGTC TCTTGACGTC CCTCTACAGC 
CAAGTCCAGC AGATTGTGAC TAACTGGCGA GAGGACCAGT ACCAGGACGA CTGCAAGGCT 
AAGCAGATGA TGCACGAGGC CTTGAAGCTG CGACTCAATC TGGTGGGTGG GATGTTTGAC 
ACTGTACAGC GTAGCACTCA GCAGACAACA GAGTGGGCAG TGCTTCTGCT GGACATCATC 
AGCAGTGGCA CAGTGGACAT GCAGTCTAAC AATGAGCTCT TCACTACGGT TTTAGACATG 
CTGAGCGTGT TGATTAACGG GACTCTGGCG GCTGACATGT CCAGCATATC TCAGGGCAGC 
ATGGAGGAGA ATAAGAGAGC TTACATGAAC CTGGTCAAGA AGCTCAGGAA AGAACTTGGT 
GACCGACAAT CCGAGAGCTT GGAGAAAGTG CGTCAGTTGC TGCCTCTGCC CAAGCAGACG 
CGTGATGTCA TCACCTGTGA GCCCCAGGGG TCACTCATTG ACACAAAGGG CAACAAAATT 
GCTGGATTTG AAAAAGAGGG TCTTCAGGTA TCAACTAAGC AGAAGATTTC TCCATGGGAT 
GTGTTTGAGG GACTAAAGCA CTCTGCTCCG CTCTCCTGGG GCTGGTTCGG AACCATACGG 
GTCGACCGCA AAGTCACTAA GTTTGAAGAG CAGCAGCGGC TCCTGCTTTA CCACACACAC 
ATCAAGCCCA AGCAACGCAG TTACTACCTG GCGCCCCTCA ACCTGCCCCA AGAAGAAGAG 
GAACCACTCA CTCCTGTGCC TCCCGAGCCA GACAAGAAGC TGGACACAGC GAAGGTGGAG 
AAAAGCGTCT CCAATGTCCC GACTAAGAAA AAGAAAAAGA AACCTGCATC GACGGTTAAA 
CAAGAGGACT ACAAGCCACA CAACACAGTC ATGCAGTACG CTCCAGGCAT GACCCCTGAT 
CTCCCACTGA GCATGGGACA AACAAACATG TCATATCGAA TGGGCTACCA AGCTCCAATG 
AACATGTATG CCCAGAACCA GCCTTTACCA CCAGGAGGTC CTGGTTTGGA GCCACCTTTT 
CGTCCTAGGG TTCCTATGAA TAAAATGCCT CCACGTCCCA ACTACACTAT GCCTAATATG 
CAGACCGCTG GCATGGGCAA CCTAATGGGA ATGGAGAAGC AGTACCAGAT GAACTATAAA 
CCCATGCCCA ACATGGCTCA GGGGCAGATG CTCCGTCATC AGCTACAGGT CAGACTGGGT 
CATAATCATT TAATTGGACA GCAGATTAGA CAAGTGACAC CTAATCCACA GTATTCATCA 
ATGCAGCCAG CACAGAATAT ACCCCAGGGC TACACCTCCT ACGGCTCACA CATGGGGATG 
CAGCCACACC CCTCTCAGAC CCCTGGAATG GTTCCCAATT CATATGGAAA CCAGGGCTTT 
CAGACAGGAC ATCCTGCCAC TAACCCAACT ATGGTGGACT CAATAAGGCA GATGCAGCAG 
AGACCTGGCT ACGTCCATCA GCAGGCTCCT GCGGCATATG GGCACACAAT CCAGAGCACA 
CAAAGGTTTC CGCATCAATC CATGCAGCAG ACACCCATGA TGCACGGCTT GGCTCAAAGT 
CACTTGGGAG TGCAGGGCAT CCACCCAAAC ATGAGGCCCA ATCAGATGAT GGAGCAGCAG 
CAGCAACAAC AACAACAACA ACAGCAACAG CAGCAACAAC AACAACAACA ACAGGTCCAC 
CAGCAGCAGC AGCACATCCG ACAGGGTCCA GTTTTTAGAC CACAGCAGCA ACAGCAGGTT 
CAGCAGCAAG TGCAACAGCA GGTCCAGCAG CAGCAGGTCC AACAGCAGCA GGTCCAGCAA 
CAGGTTCAGC CTCAGCAAGT TCAACAGCAG CAGGTTCAGC AGCAGCAGCA GCAGGTGTCT 
GCAGCTCAGC CTCCCGCTCA GGCCCTGGGC ATGCAGGCTC TGCCCCCGCA ACAGCCCATG 
TTCCAGCGGC AAGGCATGCA GCAAACCCAG CAGCAGCAGC AGACCGCAGC ACTGGTCAGG 
CAACTCCAGC AGCAGCTCTC CAGTACTCAA CCTGCACAGA ACAACAGCTC CTATTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_009291038.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289313.1 Danio rerio mediator complex subunit 12 (med12), transcript variant X2, mRNA.

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CloneID	ODa44930
Clone ID Related Accession (Same CDS sequence)	XM_009291038.3 , XM_009291038.2
Accession Version	XM_009291038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGATGTTTTC TCCTCCAATG CACTGTGAGT CGAAGGGAAG CCCTTCTCCA 
GAGAAACAAG CCCAAGAGCA GGAGAGCAAA AGCACTGCCA AGGATAAGGG CATGGACCCA 
GCCTTCCCTT TGGTTTATGA ACAGCCCCGC CACATACAGT ACGCCACCCA CTTCCCCATT 
CCTCAGGAGG AGAGTGCAAG TCATGAGTGT AACCAGCGTC TAGTGGTTCT CTATGGAGTC 
GGCAAGCAGC GAGATGAAGC TCGCCACGCC ATCAAGAAGA TCACCAAAGA CATCCTGAAG 
GTTCTCAACC GCAAGAGTAC AGCAGAGACA GGAGGTGAGG AAGGCCAGAA GAGGAAGAGG 
AGCAAACCTG AGGCCTTCCC CACTGCTGAA GATATTTTCT CTAAATTCCA GCACCTCTCA 
CACTTTGACC AGCACCAGGT CACCTCACAG GTGTCCAGGA ATGTTCTGGA GCAAATCACC 
AGCTTTGCCT TAGGGATGTC CTATCACCTC CCACTGGTCC AACACATCCA GTTCATCTTT 
GACCTCATGG AGTACTCTCT TAATATAAGT GGCCTCATTG ACTTTGCTAT ACAGCTGCTG 
AATGAATTGA GTCTGGTGGA AGCAGAACTC CTGCTTAAGT CCTCCAACCT GGCGGGCAGT 
TACACTACAG GCCTCTGTTT GTGTATCGTG GCTGTTCTGC GGAGATACCA CTCCTGCCTC 
ATCCTCAACC CTGACCAGAC CGCACAGGTC TTTGACGGGT TGCGGATTGT AGTCAAGTCT 
GGTGTGAATC CTGCAGACTG TTCCTCAGCA GAACGCTGTA TCCTAGCCTA TCTCTACGAC 
CTCTATACCT CCTGCAGTCA CCTAAAGAGC AAGTTTGGAG AGATTTTCAG TGAGTTCTGC 
TCAAAGGTGA AGAATTCCAT CTATTATAAC ATCGACCCGT CAGACTCCAA CATGCTGTGG 
GATCAGATGT TTATGATCGA TGCCATCACT AATCCTACCG CACACAACCT CAACCACTCC 
ATGTTGGGAA AGATCACCAA TGAACTCAAT GACAGCCCGG CTAACCGCTA CAGCTTTGTG 
TGTAATGTGC TCATGGATGT TTGCGTTGAC CATCGAGATC CTGAGAGGGT GAATGATATT 
GGTATCCTGT GTGCGGAGCT GACAGCATAC TGTCGCTCTC TAAGTGCTGA GTGGTTGGGT 
GTGCTCAAGG CCCTCTGCTG CTCTTCTAAC AACGGCAACT GTGGCTTCAA CGATCTGCTC 
TGCAATGTTG ACGTGAGCGA TCTATCTTTC CACGACTCTC TAGCAACATT CGTTGCCATC 
TTAATAGCTC GGCAGTGTTT GTTGCTGGAA GATCTGGTGC GCTGTGTTGC CATCCCATCC 
CTCCTGAATG CAGCCTGTAG TGAACAGGAT TCTGAACCTG GAGCCAGACT GACATGTAGG 
ATCCTGCTTC ATCTGTTCAG AACCCCACAG CGCAACCCCT GTCCCCAGGA TGGCACTAAA 
TCAGACAAAT CCATAGTGGG GATCAGGTCG TCCTGCGACC GACATCTGCT GGCTGCTTCA 
CAGAACAGCA TCGTGGTGGG AGCCGTTTTT GCTGTATTAA AAGCTGTGTT CATGCTGGGA 
GATGCTGAGC TGAAAGGCTC AGGCTTTTCC CATCCTGCTG GTCTGGATGA TATTGGAGAG 
GATGACATGG GCTCTAAAAA ATCTGGAGGA CGTAATGTCT CTATTGAAAC AGCCAGTCTG 
GTGGTTTACG CCAAGTATGT GCTGAAGAGC ATCTGCCACC AGGAATGGGT TGGCGAGCGC 
TGTCTGAAGT CTCTGTCAGA AGACAGCAGT GCACTGCAGG ATCCAGTGCT GGTCAACATC 
CAGGCTCAGC GCTTGCTGCA GCTCATCTGT TACCCACACC GACAGCTGGA CAGTGAAGAG 
GGTGACAATC CACAGAGACA GCGCATCAAA CGCATCCTGC AGAACATGGA CCAGTGGACC 
ATGAGACAGT CCTCTCTTGA GCTACAACTC ATGATCAAGC AAAGCTCAAA TAATGAGCTG 
TATTCTCTTC TGGAGAACAT AGCCAAGGCC ACTATTGAGG TTTTCCAGAA GTCTGCAGAG 
ATGAACTCTA GTAACCCCTC CTGGAATGGC TCTGCTGTCT CGGGTAGCTC AGTCTCCAAT 
AGCAACAGTG CCAGCAAACT CAAACCTGTG CTAAGTTCCT CTGAGCGTTC AGGTGTCTGG 
TTAGTTGCCC CTCTAATTGG TAAGCTGCCC ACCTCAGTGC AGGGGCACGT GCTCAAGGCT 
GCAGGCGAGG AGCTGGAGAA GGGACAGCAT CTGGGACCCT CTTCACGAAA AGAAAGAGAT 
CGACAGAAGC AAAAAAGCAT GTCTCTCCTG AGCCAGCAGC CCTTCCTGTC ACTGGTTCTG 
ACATGTCTCA AAGGGCAAGA CGAGCAGAGA GAGGGTCTCT TGACGTCCCT CTACAGCCAA 
GTCCAGCAGA TTGTGACTAA CTGGCGAGAG GACCAGTACC AGGACGACTG CAAGGCTAAG 
CAGATGATGC ACGAGGCCTT GAAGCTGCGA CTCAATCTGG TGGGTGGGAT GTTTGACACT 
GTACAGCGTA GCACTCAGCA GACAACAGAG TGGGCAGTGC TTCTGCTGGA CATCATCAGC 
AGTGGCACAG TGGACATGCA GTCTAACAAT GAGCTCTTCA CTACGGTTTT AGACATGCTG 
AGCGTGTTGA TTAACGGGAC TCTGGCGGCT GACATGTCCA GCATATCTCA GGGCAGCATG 
GAGGAGAATA AGAGAGCTTA CATGAACCTG GTCAAGAAGC TCAGGAAAGA ACTTGGTGAC 
CGACAATCCG AGAGCTTGGA GAAAGTGCGT CAGTTGCTGC CTCTGCCCAA GCAGACGCGT 
GATGTCATCA CCTGTGAGCC CCAGGGGTCA CTCATTGACA CAAAGGGCAA CAAAATTGCT 
GGATTTGAAA AAGAGGGTCT TCAGGTATCA ACTAAGCAGA AGATTTCTCC ATGGGATGTG 
TTTGAGGGAC TAAAGCACTC TGCTCCGCTC TCCTGGGGCT GGTTCGGAAC CATACGGGTC 
GACCGCAAAG TCACTAAGTT TGAAGAGCAG CAGCGGCTCC TGCTTTACCA CACACACATC 
AAGCCCAAGC AACGCAGTTA CTACCTGGCG CCCCTCAACC TGCCCCAAGA AGAAGAGGAA 
CCACTCACTC CTGTGCCTCC CGAGCCAGAC AAGAAGCTGG ACACAGCGAA GGTGGAGAAA 
AGCGTCTCCA ATGTCCCGAC TAAGAAAAAG AAAAAGAAAC CTGCATCGAC GGTTAAACAA 
GAGGACTACA AGCCACACAA CACAGTCATG CAGTACGCTC CAGGCATGAC CCCTGATCTC 
CCACTGAGCA TGGGACAAAC AAACATGTCA TATCGAATGG GCTACCAAGC TCCAATGAAC 
ATGTATGCCC AGAACCAGCC TTTACCACCA GGAGGTCCTG GTTTGGAGCC ACCTTTTCGT 
CCTAGGGTTC CTATGAATAA AATGCCTCCA CGTCCCAACT ACACTATGCC TAATATGCAG 
ACCGCTGGCA TGGGCAACCT AATGGGAATG GAGAAGCAGT ACCAGATGAA CTATAAACCC 
ATGCCCAACA TGGCTCAGGG GCAGATGCTC CGTCATCAGC TACAGGTCAG ACTGGGTCAT 
AATCATTTAA TTGGACAGCA GATTAGACAA GTGACACCTA ATCCACAGTA TTCATCAATG 
CAGCCAGCAC AGAATATACC CCAGGGCTAC ACCTCCTACG GCTCACACAT GGGGATGCAG 
CCACACCCCT CTCAGACCCC TGGAATGGTT CCCAATTCAT ATGGAAACCA GGGCTTTCAG 
ACAGGACATC CTGCCACTAA CCCAACTATG GTGGACTCAA TAAGGCAGAT GCAGCAGAGA 
CCTGGCTACG TCCATCAGCA GGCTCCTGCG GCATATGGGC ACACAATCCA GAGCACACAA 
AGGTTTCCGC ATCAATCCAT GCAGCAGACA CCCATGATGC ACGGCTTGGC TCAAAGTCAC 
TTGGGAGTGC AGGGCATCCA CCCAAACATG AGGCCCAATC AGATGATGGA GCAGCAGCAG 
CAACAACAAC AACAACAACA GCAACAGCAG CAACAACAAC AACAACAACA GGTCCACCAG 
CAGCAGCAGC ACATCCGACA GGGTCCAGTT TTTAGACCAC AGCAGCAACA GCAGGTTCAG 
CAGCAAGTGC AACAGCAGGT CCAGCAGCAG CAGGTCCAAC AGCAGCAGGT CCAGCAACAG 
GTTCAGCCTC AGCAAGTTCA ACAGCAGCAG GTTCAGCAGC AGCAGCAGCA GGTGTCTGCA 
GCTCAGCCTC CCGCTCAGGC CCTGGGCATG CAGGCTCTGC CCCCGCAACA GCCCATGTTC 
CAGCGGCAAG GCATGCAGCA AACCCAGCAG CAGCAGCAGA CCGCAGCACT GGTCAGGCAA 
CTCCAGCAGC AGCTCTCCAG TACTCAACCT GCACAGAACA ACAGCTCCTA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289313.1
CDS	95..6631
Translation	MMAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNSSKISSNFSSIIAEKLRCNTFPDTGKRKPQVNQKDNFWLVTARSQSSINNWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYSVPVMRSAWMIKMTCAYHAAITENKMKKRHVIDPCIEWTQIITKYLWEQLQKVAEFYRQSPSQGCGSPLPAPPAEVETAMKQWEYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLRLLLPLLLQYSGEFVQSAYLSRRLAYFCTRRLNLLLSDGSIGPGPGGHQAHGISAQQGNALPPTPTSQPAGGNQPQTPFTDFYICPQHRPVVFGLSCMLQSIVLCCPSALVWHYSLTDSRNKTGSPLDLLPIAPSNLPMPGGNSTFNQQVRAKVREIEEQIKERGQAVEFRWSFDKCQETTAGFTISRVLHTLEVLDNHSFEKSDFSNSLDSLYNRIFGSGQSKDGHEMSPDDDAVVTLLCEWAVSCKRSGPHRAMVVAKLLEKRQTEIEAERCGESEVVDEKGSVSSGSLSAATLPVFQDVLLQFLDTQAPVLTEPGNESERVEFSNLVLLFYELIRHDVFSHNIYMCTLISRGDLASNSHLPRPRSPSDEPSDESERKDQDAGSSVKIEDTGMSESMEIDHNSSANFDEMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESASHECNQRLVVLYGVGKQRDEARHAIKKITKDILKVLNRKSTAETGGEEGQKRKRSKPEAFPTAEDIFSKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSLVEAELLLKSSNLAGSYTTGLCLCIVAVLRRYHSCLILNPDQTAQVFDGLRIVVKSGVNPADCSSAERCILAYLYDLYTSCSHLKSKFGEIFSEFCSKVKNSIYYNIDPSDSNMLWDQMFMIDAITNPTAHNLNHSMLGKITNELNDSPANRYSFVCNVLMDVCVDHRDPERVNDIGILCAELTAYCRSLSAEWLGVLKALCCSSNNGNCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLVRCVAIPSLLNAACSEQDSEPGARLTCRILLHLFRTPQRNPCPQDGTKSDKSIVGIRSSCDRHLLAASQNSIVVGAVFAVLKAVFMLGDAELKGSGFSHPAGLDDIGEDDMGSKKSGGRNVSIETASLVVYAKYVLKSICHQEWVGERCLKSLSEDSSALQDPVLVNIQAQRLLQLICYPHRQLDSEEGDNPQRQRIKRILQNMDQWTMRQSSLELQLMIKQSSNNELYSLLENIAKATIEVFQKSAEMNSSNPSWNGSAVSGSSVSNSNSASKLKPVLSSSERSGVWLVAPLIGKLPTSVQGHVLKAAGEELEKGQHLGPSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQMMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFEKEGLQVSTKQKISPWDVFEGLKHSAPLSWGWFGTIRVDRKVTKFEEQQRLLLYHTHIKPKQRSYYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMSYRMGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQYQMNYKPMPNMAQGQMLRHQLQVRLGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQQQQQVQQQVQQQVQQQQVQQQQVQQQVQPQQVQQQQVQQQQQQVSAAQPPAQALGMQALPPQQPMFQRQGMQQTQQQQQTAALVRQLQQQLSSTQPAQNNSSYY

Target ORF information:

RefSeq Version	XM_009291038.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mediator complex subunit 12 (med12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291038.3

ORF Insert Sequence:

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGATGTTTTC TCCTCCAATG CACTGTGAGT CGAAGGGAAG CCCTTCTCCA 
GAGAAACAAG CCCAAGAGCA GGAGAGCAAA AGCACTGCCA AGGATAAGGG CATGGACCCA 
GCCTTCCCTT TGGTTTATGA ACAGCCCCGC CACATACAGT ACGCCACCCA CTTCCCCATT 
CCTCAGGAGG AGAGTGCAAG TCATGAGTGT AACCAGCGTC TAGTGGTTCT CTATGGAGTC 
GGCAAGCAGC GAGATGAAGC TCGCCACGCC ATCAAGAAGA TCACCAAAGA CATCCTGAAG 
GTTCTCAACC GCAAGAGTAC AGCAGAGACA GGAGGTGAGG AAGGCCAGAA GAGGAAGAGG 
AGCAAACCTG AGGCCTTCCC CACTGCTGAA GATATTTTCT CTAAATTCCA GCACCTCTCA 
CACTTTGACC AGCACCAGGT CACCTCACAG GTGTCCAGGA ATGTTCTGGA GCAAATCACC 
AGCTTTGCCT TAGGGATGTC CTATCACCTC CCACTGGTCC AACACATCCA GTTCATCTTT 
GACCTCATGG AGTACTCTCT TAATATAAGT GGCCTCATTG ACTTTGCTAT ACAGCTGCTG 
AATGAATTGA GTCTGGTGGA AGCAGAACTC CTGCTTAAGT CCTCCAACCT GGCGGGCAGT 
TACACTACAG GCCTCTGTTT GTGTATCGTG GCTGTTCTGC GGAGATACCA CTCCTGCCTC 
ATCCTCAACC CTGACCAGAC CGCACAGGTC TTTGACGGGT TGCGGATTGT AGTCAAGTCT 
GGTGTGAATC CTGCAGACTG TTCCTCAGCA GAACGCTGTA TCCTAGCCTA TCTCTACGAC 
CTCTATACCT CCTGCAGTCA CCTAAAGAGC AAGTTTGGAG AGATTTTCAG TGAGTTCTGC 
TCAAAGGTGA AGAATTCCAT CTATTATAAC ATCGACCCGT CAGACTCCAA CATGCTGTGG 
GATCAGATGT TTATGATCGA TGCCATCACT AATCCTACCG CACACAACCT CAACCACTCC 
ATGTTGGGAA AGATCACCAA TGAACTCAAT GACAGCCCGG CTAACCGCTA CAGCTTTGTG 
TGTAATGTGC TCATGGATGT TTGCGTTGAC CATCGAGATC CTGAGAGGGT GAATGATATT 
GGTATCCTGT GTGCGGAGCT GACAGCATAC TGTCGCTCTC TAAGTGCTGA GTGGTTGGGT 
GTGCTCAAGG CCCTCTGCTG CTCTTCTAAC AACGGCAACT GTGGCTTCAA CGATCTGCTC 
TGCAATGTTG ACGTGAGCGA TCTATCTTTC CACGACTCTC TAGCAACATT CGTTGCCATC 
TTAATAGCTC GGCAGTGTTT GTTGCTGGAA GATCTGGTGC GCTGTGTTGC CATCCCATCC 
CTCCTGAATG CAGCCTGTAG TGAACAGGAT TCTGAACCTG GAGCCAGACT GACATGTAGG 
ATCCTGCTTC ATCTGTTCAG AACCCCACAG CGCAACCCCT GTCCCCAGGA TGGCACTAAA 
TCAGACAAAT CCATAGTGGG GATCAGGTCG TCCTGCGACC GACATCTGCT GGCTGCTTCA 
CAGAACAGCA TCGTGGTGGG AGCCGTTTTT GCTGTATTAA AAGCTGTGTT CATGCTGGGA 
GATGCTGAGC TGAAAGGCTC AGGCTTTTCC CATCCTGCTG GTCTGGATGA TATTGGAGAG 
GATGACATGG GCTCTAAAAA ATCTGGAGGA CGTAATGTCT CTATTGAAAC AGCCAGTCTG 
GTGGTTTACG CCAAGTATGT GCTGAAGAGC ATCTGCCACC AGGAATGGGT TGGCGAGCGC 
TGTCTGAAGT CTCTGTCAGA AGACAGCAGT GCACTGCAGG ATCCAGTGCT GGTCAACATC 
CAGGCTCAGC GCTTGCTGCA GCTCATCTGT TACCCACACC GACAGCTGGA CAGTGAAGAG 
GGTGACAATC CACAGAGACA GCGCATCAAA CGCATCCTGC AGAACATGGA CCAGTGGACC 
ATGAGACAGT CCTCTCTTGA GCTACAACTC ATGATCAAGC AAAGCTCAAA TAATGAGCTG 
TATTCTCTTC TGGAGAACAT AGCCAAGGCC ACTATTGAGG TTTTCCAGAA GTCTGCAGAG 
ATGAACTCTA GTAACCCCTC CTGGAATGGC TCTGCTGTCT CGGGTAGCTC AGTCTCCAAT 
AGCAACAGTG CCAGCAAACT CAAACCTGTG CTAAGTTCCT CTGAGCGTTC AGGTGTCTGG 
TTAGTTGCCC CTCTAATTGG TAAGCTGCCC ACCTCAGTGC AGGGGCACGT GCTCAAGGCT 
GCAGGCGAGG AGCTGGAGAA GGGACAGCAT CTGGGACCCT CTTCACGAAA AGAAAGAGAT 
CGACAGAAGC AAAAAAGCAT GTCTCTCCTG AGCCAGCAGC CCTTCCTGTC ACTGGTTCTG 
ACATGTCTCA AAGGGCAAGA CGAGCAGAGA GAGGGTCTCT TGACGTCCCT CTACAGCCAA 
GTCCAGCAGA TTGTGACTAA CTGGCGAGAG GACCAGTACC AGGACGACTG CAAGGCTAAG 
CAGATGATGC ACGAGGCCTT GAAGCTGCGA CTCAATCTGG TGGGTGGGAT GTTTGACACT 
GTACAGCGTA GCACTCAGCA GACAACAGAG TGGGCAGTGC TTCTGCTGGA CATCATCAGC 
AGTGGCACAG TGGACATGCA GTCTAACAAT GAGCTCTTCA CTACGGTTTT AGACATGCTG 
AGCGTGTTGA TTAACGGGAC TCTGGCGGCT GACATGTCCA GCATATCTCA GGGCAGCATG 
GAGGAGAATA AGAGAGCTTA CATGAACCTG GTCAAGAAGC TCAGGAAAGA ACTTGGTGAC 
CGACAATCCG AGAGCTTGGA GAAAGTGCGT CAGTTGCTGC CTCTGCCCAA GCAGACGCGT 
GATGTCATCA CCTGTGAGCC CCAGGGGTCA CTCATTGACA CAAAGGGCAA CAAAATTGCT 
GGATTTGAAA AAGAGGGTCT TCAGGTATCA ACTAAGCAGA AGATTTCTCC ATGGGATGTG 
TTTGAGGGAC TAAAGCACTC TGCTCCGCTC TCCTGGGGCT GGTTCGGAAC CATACGGGTC 
GACCGCAAAG TCACTAAGTT TGAAGAGCAG CAGCGGCTCC TGCTTTACCA CACACACATC 
AAGCCCAAGC AACGCAGTTA CTACCTGGCG CCCCTCAACC TGCCCCAAGA AGAAGAGGAA 
CCACTCACTC CTGTGCCTCC CGAGCCAGAC AAGAAGCTGG ACACAGCGAA GGTGGAGAAA 
AGCGTCTCCA ATGTCCCGAC TAAGAAAAAG AAAAAGAAAC CTGCATCGAC GGTTAAACAA 
GAGGACTACA AGCCACACAA CACAGTCATG CAGTACGCTC CAGGCATGAC CCCTGATCTC 
CCACTGAGCA TGGGACAAAC AAACATGTCA TATCGAATGG GCTACCAAGC TCCAATGAAC 
ATGTATGCCC AGAACCAGCC TTTACCACCA GGAGGTCCTG GTTTGGAGCC ACCTTTTCGT 
CCTAGGGTTC CTATGAATAA AATGCCTCCA CGTCCCAACT ACACTATGCC TAATATGCAG 
ACCGCTGGCA TGGGCAACCT AATGGGAATG GAGAAGCAGT ACCAGATGAA CTATAAACCC 
ATGCCCAACA TGGCTCAGGG GCAGATGCTC CGTCATCAGC TACAGGTCAG ACTGGGTCAT 
AATCATTTAA TTGGACAGCA GATTAGACAA GTGACACCTA ATCCACAGTA TTCATCAATG 
CAGCCAGCAC AGAATATACC CCAGGGCTAC ACCTCCTACG GCTCACACAT GGGGATGCAG 
CCACACCCCT CTCAGACCCC TGGAATGGTT CCCAATTCAT ATGGAAACCA GGGCTTTCAG 
ACAGGACATC CTGCCACTAA CCCAACTATG GTGGACTCAA TAAGGCAGAT GCAGCAGAGA 
CCTGGCTACG TCCATCAGCA GGCTCCTGCG GCATATGGGC ACACAATCCA GAGCACACAA 
AGGTTTCCGC ATCAATCCAT GCAGCAGACA CCCATGATGC ACGGCTTGGC TCAAAGTCAC 
TTGGGAGTGC AGGGCATCCA CCCAAACATG AGGCCCAATC AGATGATGGA GCAGCAGCAG 
CAACAACAAC AACAACAACA GCAACAGCAG CAACAACAAC AACAACAACA GGTCCACCAG 
CAGCAGCAGC ACATCCGACA GGGTCCAGTT TTTAGACCAC AGCAGCAACA GCAGGTTCAG 
CAGCAAGTGC AACAGCAGGT CCAGCAGCAG CAGGTCCAAC AGCAGCAGGT CCAGCAACAG 
GTTCAGCCTC AGCAAGTTCA ACAGCAGCAG GTTCAGCAGC AGCAGCAGCA GGTGTCTGCA 
GCTCAGCCTC CCGCTCAGGC CCTGGGCATG CAGGCTCTGC CCCCGCAACA GCCCATGTTC 
CAGCGGCAAG GCATGCAGCA AACCCAGCAG CAGCAGCAGA CCGCAGCACT GGTCAGGCAA 
CTCCAGCAGC AGCTCTCCAG TACTCAACCT GCACAGAACA ACAGCTCCTA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_009291038.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289313.1 Danio rerio mediator complex subunit 12 (med12), transcript variant X2, mRNA.

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CloneID	ODa44930
Clone ID Related Accession (Same CDS sequence)	XM_009291038.3 , XM_009291038.2
Accession Version	XM_009291038.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6537bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGATGTTTTC TCCTCCAATG CACTGTGAGT CGAAGGGAAG CCCTTCTCCA 
GAGAAACAAG CCCAAGAGCA GGAGAGCAAA AGCACTGCCA AGGATAAGGG CATGGACCCA 
GCCTTCCCTT TGGTTTATGA ACAGCCCCGC CACATACAGT ACGCCACCCA CTTCCCCATT 
CCTCAGGAGG AGAGTGCAAG TCATGAGTGT AACCAGCGTC TAGTGGTTCT CTATGGAGTC 
GGCAAGCAGC GAGATGAAGC TCGCCACGCC ATCAAGAAGA TCACCAAAGA CATCCTGAAG 
GTTCTCAACC GCAAGAGTAC AGCAGAGACA GGAGGTGAGG AAGGCCAGAA GAGGAAGAGG 
AGCAAACCTG AGGCCTTCCC CACTGCTGAA GATATTTTCT CTAAATTCCA GCACCTCTCA 
CACTTTGACC AGCACCAGGT CACCTCACAG GTGTCCAGGA ATGTTCTGGA GCAAATCACC 
AGCTTTGCCT TAGGGATGTC CTATCACCTC CCACTGGTCC AACACATCCA GTTCATCTTT 
GACCTCATGG AGTACTCTCT TAATATAAGT GGCCTCATTG ACTTTGCTAT ACAGCTGCTG 
AATGAATTGA GTCTGGTGGA AGCAGAACTC CTGCTTAAGT CCTCCAACCT GGCGGGCAGT 
TACACTACAG GCCTCTGTTT GTGTATCGTG GCTGTTCTGC GGAGATACCA CTCCTGCCTC 
ATCCTCAACC CTGACCAGAC CGCACAGGTC TTTGACGGGT TGCGGATTGT AGTCAAGTCT 
GGTGTGAATC CTGCAGACTG TTCCTCAGCA GAACGCTGTA TCCTAGCCTA TCTCTACGAC 
CTCTATACCT CCTGCAGTCA CCTAAAGAGC AAGTTTGGAG AGATTTTCAG TGAGTTCTGC 
TCAAAGGTGA AGAATTCCAT CTATTATAAC ATCGACCCGT CAGACTCCAA CATGCTGTGG 
GATCAGATGT TTATGATCGA TGCCATCACT AATCCTACCG CACACAACCT CAACCACTCC 
ATGTTGGGAA AGATCACCAA TGAACTCAAT GACAGCCCGG CTAACCGCTA CAGCTTTGTG 
TGTAATGTGC TCATGGATGT TTGCGTTGAC CATCGAGATC CTGAGAGGGT GAATGATATT 
GGTATCCTGT GTGCGGAGCT GACAGCATAC TGTCGCTCTC TAAGTGCTGA GTGGTTGGGT 
GTGCTCAAGG CCCTCTGCTG CTCTTCTAAC AACGGCAACT GTGGCTTCAA CGATCTGCTC 
TGCAATGTTG ACGTGAGCGA TCTATCTTTC CACGACTCTC TAGCAACATT CGTTGCCATC 
TTAATAGCTC GGCAGTGTTT GTTGCTGGAA GATCTGGTGC GCTGTGTTGC CATCCCATCC 
CTCCTGAATG CAGCCTGTAG TGAACAGGAT TCTGAACCTG GAGCCAGACT GACATGTAGG 
ATCCTGCTTC ATCTGTTCAG AACCCCACAG CGCAACCCCT GTCCCCAGGA TGGCACTAAA 
TCAGACAAAT CCATAGTGGG GATCAGGTCG TCCTGCGACC GACATCTGCT GGCTGCTTCA 
CAGAACAGCA TCGTGGTGGG AGCCGTTTTT GCTGTATTAA AAGCTGTGTT CATGCTGGGA 
GATGCTGAGC TGAAAGGCTC AGGCTTTTCC CATCCTGCTG GTCTGGATGA TATTGGAGAG 
GATGACATGG GCTCTAAAAA ATCTGGAGGA CGTAATGTCT CTATTGAAAC AGCCAGTCTG 
GTGGTTTACG CCAAGTATGT GCTGAAGAGC ATCTGCCACC AGGAATGGGT TGGCGAGCGC 
TGTCTGAAGT CTCTGTCAGA AGACAGCAGT GCACTGCAGG ATCCAGTGCT GGTCAACATC 
CAGGCTCAGC GCTTGCTGCA GCTCATCTGT TACCCACACC GACAGCTGGA CAGTGAAGAG 
GGTGACAATC CACAGAGACA GCGCATCAAA CGCATCCTGC AGAACATGGA CCAGTGGACC 
ATGAGACAGT CCTCTCTTGA GCTACAACTC ATGATCAAGC AAAGCTCAAA TAATGAGCTG 
TATTCTCTTC TGGAGAACAT AGCCAAGGCC ACTATTGAGG TTTTCCAGAA GTCTGCAGAG 
ATGAACTCTA GTAACCCCTC CTGGAATGGC TCTGCTGTCT CGGGTAGCTC AGTCTCCAAT 
AGCAACAGTG CCAGCAAACT CAAACCTGTG CTAAGTTCCT CTGAGCGTTC AGGTGTCTGG 
TTAGTTGCCC CTCTAATTGG TAAGCTGCCC ACCTCAGTGC AGGGGCACGT GCTCAAGGCT 
GCAGGCGAGG AGCTGGAGAA GGGACAGCAT CTGGGACCCT CTTCACGAAA AGAAAGAGAT 
CGACAGAAGC AAAAAAGCAT GTCTCTCCTG AGCCAGCAGC CCTTCCTGTC ACTGGTTCTG 
ACATGTCTCA AAGGGCAAGA CGAGCAGAGA GAGGGTCTCT TGACGTCCCT CTACAGCCAA 
GTCCAGCAGA TTGTGACTAA CTGGCGAGAG GACCAGTACC AGGACGACTG CAAGGCTAAG 
CAGATGATGC ACGAGGCCTT GAAGCTGCGA CTCAATCTGG TGGGTGGGAT GTTTGACACT 
GTACAGCGTA GCACTCAGCA GACAACAGAG TGGGCAGTGC TTCTGCTGGA CATCATCAGC 
AGTGGCACAG TGGACATGCA GTCTAACAAT GAGCTCTTCA CTACGGTTTT AGACATGCTG 
AGCGTGTTGA TTAACGGGAC TCTGGCGGCT GACATGTCCA GCATATCTCA GGGCAGCATG 
GAGGAGAATA AGAGAGCTTA CATGAACCTG GTCAAGAAGC TCAGGAAAGA ACTTGGTGAC 
CGACAATCCG AGAGCTTGGA GAAAGTGCGT CAGTTGCTGC CTCTGCCCAA GCAGACGCGT 
GATGTCATCA CCTGTGAGCC CCAGGGGTCA CTCATTGACA CAAAGGGCAA CAAAATTGCT 
GGATTTGAAA AAGAGGGTCT TCAGGTATCA ACTAAGCAGA AGATTTCTCC ATGGGATGTG 
TTTGAGGGAC TAAAGCACTC TGCTCCGCTC TCCTGGGGCT GGTTCGGAAC CATACGGGTC 
GACCGCAAAG TCACTAAGTT TGAAGAGCAG CAGCGGCTCC TGCTTTACCA CACACACATC 
AAGCCCAAGC AACGCAGTTA CTACCTGGCG CCCCTCAACC TGCCCCAAGA AGAAGAGGAA 
CCACTCACTC CTGTGCCTCC CGAGCCAGAC AAGAAGCTGG ACACAGCGAA GGTGGAGAAA 
AGCGTCTCCA ATGTCCCGAC TAAGAAAAAG AAAAAGAAAC CTGCATCGAC GGTTAAACAA 
GAGGACTACA AGCCACACAA CACAGTCATG CAGTACGCTC CAGGCATGAC CCCTGATCTC 
CCACTGAGCA TGGGACAAAC AAACATGTCA TATCGAATGG GCTACCAAGC TCCAATGAAC 
ATGTATGCCC AGAACCAGCC TTTACCACCA GGAGGTCCTG GTTTGGAGCC ACCTTTTCGT 
CCTAGGGTTC CTATGAATAA AATGCCTCCA CGTCCCAACT ACACTATGCC TAATATGCAG 
ACCGCTGGCA TGGGCAACCT AATGGGAATG GAGAAGCAGT ACCAGATGAA CTATAAACCC 
ATGCCCAACA TGGCTCAGGG GCAGATGCTC CGTCATCAGC TACAGGTCAG ACTGGGTCAT 
AATCATTTAA TTGGACAGCA GATTAGACAA GTGACACCTA ATCCACAGTA TTCATCAATG 
CAGCCAGCAC AGAATATACC CCAGGGCTAC ACCTCCTACG GCTCACACAT GGGGATGCAG 
CCACACCCCT CTCAGACCCC TGGAATGGTT CCCAATTCAT ATGGAAACCA GGGCTTTCAG 
ACAGGACATC CTGCCACTAA CCCAACTATG GTGGACTCAA TAAGGCAGAT GCAGCAGAGA 
CCTGGCTACG TCCATCAGCA GGCTCCTGCG GCATATGGGC ACACAATCCA GAGCACACAA 
AGGTTTCCGC ATCAATCCAT GCAGCAGACA CCCATGATGC ACGGCTTGGC TCAAAGTCAC 
TTGGGAGTGC AGGGCATCCA CCCAAACATG AGGCCCAATC AGATGATGGA GCAGCAGCAG 
CAACAACAAC AACAACAACA GCAACAGCAG CAACAACAAC AACAACAACA GGTCCACCAG 
CAGCAGCAGC ACATCCGACA GGGTCCAGTT TTTAGACCAC AGCAGCAACA GCAGGTTCAG 
CAGCAAGTGC AACAGCAGGT CCAGCAGCAG CAGGTCCAAC AGCAGCAGGT CCAGCAACAG 
GTTCAGCCTC AGCAAGTTCA ACAGCAGCAG GTTCAGCAGC AGCAGCAGCA GGTGTCTGCA 
GCTCAGCCTC CCGCTCAGGC CCTGGGCATG CAGGCTCTGC CCCCGCAACA GCCCATGTTC 
CAGCGGCAAG GCATGCAGCA AACCCAGCAG CAGCAGCAGA CCGCAGCACT GGTCAGGCAA 
CTCCAGCAGC AGCTCTCCAG TACTCAACCT GCACAGAACA ACAGCTCCTA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289313.1
CDS	92..6628
Translation	MMAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNSSKISSNFSSIIAEKLRCNTFPDTGKRKPQVNQKDNFWLVTARSQSSINNWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYSVPVMRSAWMIKMTCAYHAAITENKMKKRHVIDPCIEWTQIITKYLWEQLQKVAEFYRQSPSQGCGSPLPAPPAEVETAMKQWEYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLRLLLPLLLQYSGEFVQSAYLSRRLAYFCTRRLNLLLSDGSIGPGPGGHQAHGISAQQGNALPPTPTSQPAGGNQPQTPFTDFYICPQHRPVVFGLSCMLQSIVLCCPSALVWHYSLTDSRNKTGSPLDLLPIAPSNLPMPGGNSTFNQQVRAKVREIEEQIKERGQAVEFRWSFDKCQETTAGFTISRVLHTLEVLDNHSFEKSDFSNSLDSLYNRIFGSGQSKDGHEMSPDDDAVVTLLCEWAVSCKRSGPHRAMVVAKLLEKRQTEIEAERCGESEVVDEKGSVSSGSLSAATLPVFQDVLLQFLDTQAPVLTEPGNESERVEFSNLVLLFYELIRHDVFSHNIYMCTLISRGDLASNSHLPRPRSPSDEPSDESERKDQDAGSSVKIEDTGMSESMEIDHNSSANFDEMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESASHECNQRLVVLYGVGKQRDEARHAIKKITKDILKVLNRKSTAETGGEEGQKRKRSKPEAFPTAEDIFSKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSLVEAELLLKSSNLAGSYTTGLCLCIVAVLRRYHSCLILNPDQTAQVFDGLRIVVKSGVNPADCSSAERCILAYLYDLYTSCSHLKSKFGEIFSEFCSKVKNSIYYNIDPSDSNMLWDQMFMIDAITNPTAHNLNHSMLGKITNELNDSPANRYSFVCNVLMDVCVDHRDPERVNDIGILCAELTAYCRSLSAEWLGVLKALCCSSNNGNCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLVRCVAIPSLLNAACSEQDSEPGARLTCRILLHLFRTPQRNPCPQDGTKSDKSIVGIRSSCDRHLLAASQNSIVVGAVFAVLKAVFMLGDAELKGSGFSHPAGLDDIGEDDMGSKKSGGRNVSIETASLVVYAKYVLKSICHQEWVGERCLKSLSEDSSALQDPVLVNIQAQRLLQLICYPHRQLDSEEGDNPQRQRIKRILQNMDQWTMRQSSLELQLMIKQSSNNELYSLLENIAKATIEVFQKSAEMNSSNPSWNGSAVSGSSVSNSNSASKLKPVLSSSERSGVWLVAPLIGKLPTSVQGHVLKAAGEELEKGQHLGPSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQMMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFEKEGLQVSTKQKISPWDVFEGLKHSAPLSWGWFGTIRVDRKVTKFEEQQRLLLYHTHIKPKQRSYYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMSYRMGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQYQMNYKPMPNMAQGQMLRHQLQVRLGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQQQQQVQQQVQQQVQQQQVQQQQVQQQVQPQQVQQQQVQQQQQQVSAAQPPAQALGMQALPPQQPMFQRQGMQQTQQQQQTAALVRQLQQQLSSTQPAQNNSSYY

Target ORF information:

RefSeq Version	XM_009291038.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 12 (med12), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291038.2

ORF Insert Sequence:

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGATGTTTTC TCCTCCAATG CACTGTGAGT CGAAGGGAAG CCCTTCTCCA 
GAGAAACAAG CCCAAGAGCA GGAGAGCAAA AGCACTGCCA AGGATAAGGG CATGGACCCA 
GCCTTCCCTT TGGTTTATGA ACAGCCCCGC CACATACAGT ACGCCACCCA CTTCCCCATT 
CCTCAGGAGG AGAGTGCAAG TCATGAGTGT AACCAGCGTC TAGTGGTTCT CTATGGAGTC 
GGCAAGCAGC GAGATGAAGC TCGCCACGCC ATCAAGAAGA TCACCAAAGA CATCCTGAAG 
GTTCTCAACC GCAAGAGTAC AGCAGAGACA GGAGGTGAGG AAGGCCAGAA GAGGAAGAGG 
AGCAAACCTG AGGCCTTCCC CACTGCTGAA GATATTTTCT CTAAATTCCA GCACCTCTCA 
CACTTTGACC AGCACCAGGT CACCTCACAG GTGTCCAGGA ATGTTCTGGA GCAAATCACC 
AGCTTTGCCT TAGGGATGTC CTATCACCTC CCACTGGTCC AACACATCCA GTTCATCTTT 
GACCTCATGG AGTACTCTCT TAATATAAGT GGCCTCATTG ACTTTGCTAT ACAGCTGCTG 
AATGAATTGA GTCTGGTGGA AGCAGAACTC CTGCTTAAGT CCTCCAACCT GGCGGGCAGT 
TACACTACAG GCCTCTGTTT GTGTATCGTG GCTGTTCTGC GGAGATACCA CTCCTGCCTC 
ATCCTCAACC CTGACCAGAC CGCACAGGTC TTTGACGGGT TGCGGATTGT AGTCAAGTCT 
GGTGTGAATC CTGCAGACTG TTCCTCAGCA GAACGCTGTA TCCTAGCCTA TCTCTACGAC 
CTCTATACCT CCTGCAGTCA CCTAAAGAGC AAGTTTGGAG AGATTTTCAG TGAGTTCTGC 
TCAAAGGTGA AGAATTCCAT CTATTATAAC ATCGACCCGT CAGACTCCAA CATGCTGTGG 
GATCAGATGT TTATGATCGA TGCCATCACT AATCCTACCG CACACAACCT CAACCACTCC 
ATGTTGGGAA AGATCACCAA TGAACTCAAT GACAGCCCGG CTAACCGCTA CAGCTTTGTG 
TGTAATGTGC TCATGGATGT TTGCGTTGAC CATCGAGATC CTGAGAGGGT GAATGATATT 
GGTATCCTGT GTGCGGAGCT GACAGCATAC TGTCGCTCTC TAAGTGCTGA GTGGTTGGGT 
GTGCTCAAGG CCCTCTGCTG CTCTTCTAAC AACGGCAACT GTGGCTTCAA CGATCTGCTC 
TGCAATGTTG ACGTGAGCGA TCTATCTTTC CACGACTCTC TAGCAACATT CGTTGCCATC 
TTAATAGCTC GGCAGTGTTT GTTGCTGGAA GATCTGGTGC GCTGTGTTGC CATCCCATCC 
CTCCTGAATG CAGCCTGTAG TGAACAGGAT TCTGAACCTG GAGCCAGACT GACATGTAGG 
ATCCTGCTTC ATCTGTTCAG AACCCCACAG CGCAACCCCT GTCCCCAGGA TGGCACTAAA 
TCAGACAAAT CCATAGTGGG GATCAGGTCG TCCTGCGACC GACATCTGCT GGCTGCTTCA 
CAGAACAGCA TCGTGGTGGG AGCCGTTTTT GCTGTATTAA AAGCTGTGTT CATGCTGGGA 
GATGCTGAGC TGAAAGGCTC AGGCTTTTCC CATCCTGCTG GTCTGGATGA TATTGGAGAG 
GATGACATGG GCTCTAAAAA ATCTGGAGGA CGTAATGTCT CTATTGAAAC AGCCAGTCTG 
GTGGTTTACG CCAAGTATGT GCTGAAGAGC ATCTGCCACC AGGAATGGGT TGGCGAGCGC 
TGTCTGAAGT CTCTGTCAGA AGACAGCAGT GCACTGCAGG ATCCAGTGCT GGTCAACATC 
CAGGCTCAGC GCTTGCTGCA GCTCATCTGT TACCCACACC GACAGCTGGA CAGTGAAGAG 
GGTGACAATC CACAGAGACA GCGCATCAAA CGCATCCTGC AGAACATGGA CCAGTGGACC 
ATGAGACAGT CCTCTCTTGA GCTACAACTC ATGATCAAGC AAAGCTCAAA TAATGAGCTG 
TATTCTCTTC TGGAGAACAT AGCCAAGGCC ACTATTGAGG TTTTCCAGAA GTCTGCAGAG 
ATGAACTCTA GTAACCCCTC CTGGAATGGC TCTGCTGTCT CGGGTAGCTC AGTCTCCAAT 
AGCAACAGTG CCAGCAAACT CAAACCTGTG CTAAGTTCCT CTGAGCGTTC AGGTGTCTGG 
TTAGTTGCCC CTCTAATTGG TAAGCTGCCC ACCTCAGTGC AGGGGCACGT GCTCAAGGCT 
GCAGGCGAGG AGCTGGAGAA GGGACAGCAT CTGGGACCCT CTTCACGAAA AGAAAGAGAT 
CGACAGAAGC AAAAAAGCAT GTCTCTCCTG AGCCAGCAGC CCTTCCTGTC ACTGGTTCTG 
ACATGTCTCA AAGGGCAAGA CGAGCAGAGA GAGGGTCTCT TGACGTCCCT CTACAGCCAA 
GTCCAGCAGA TTGTGACTAA CTGGCGAGAG GACCAGTACC AGGACGACTG CAAGGCTAAG 
CAGATGATGC ACGAGGCCTT GAAGCTGCGA CTCAATCTGG TGGGTGGGAT GTTTGACACT 
GTACAGCGTA GCACTCAGCA GACAACAGAG TGGGCAGTGC TTCTGCTGGA CATCATCAGC 
AGTGGCACAG TGGACATGCA GTCTAACAAT GAGCTCTTCA CTACGGTTTT AGACATGCTG 
AGCGTGTTGA TTAACGGGAC TCTGGCGGCT GACATGTCCA GCATATCTCA GGGCAGCATG 
GAGGAGAATA AGAGAGCTTA CATGAACCTG GTCAAGAAGC TCAGGAAAGA ACTTGGTGAC 
CGACAATCCG AGAGCTTGGA GAAAGTGCGT CAGTTGCTGC CTCTGCCCAA GCAGACGCGT 
GATGTCATCA CCTGTGAGCC CCAGGGGTCA CTCATTGACA CAAAGGGCAA CAAAATTGCT 
GGATTTGAAA AAGAGGGTCT TCAGGTATCA ACTAAGCAGA AGATTTCTCC ATGGGATGTG 
TTTGAGGGAC TAAAGCACTC TGCTCCGCTC TCCTGGGGCT GGTTCGGAAC CATACGGGTC 
GACCGCAAAG TCACTAAGTT TGAAGAGCAG CAGCGGCTCC TGCTTTACCA CACACACATC 
AAGCCCAAGC AACGCAGTTA CTACCTGGCG CCCCTCAACC TGCCCCAAGA AGAAGAGGAA 
CCACTCACTC CTGTGCCTCC CGAGCCAGAC AAGAAGCTGG ACACAGCGAA GGTGGAGAAA 
AGCGTCTCCA ATGTCCCGAC TAAGAAAAAG AAAAAGAAAC CTGCATCGAC GGTTAAACAA 
GAGGACTACA AGCCACACAA CACAGTCATG CAGTACGCTC CAGGCATGAC CCCTGATCTC 
CCACTGAGCA TGGGACAAAC AAACATGTCA TATCGAATGG GCTACCAAGC TCCAATGAAC 
ATGTATGCCC AGAACCAGCC TTTACCACCA GGAGGTCCTG GTTTGGAGCC ACCTTTTCGT 
CCTAGGGTTC CTATGAATAA AATGCCTCCA CGTCCCAACT ACACTATGCC TAATATGCAG 
ACCGCTGGCA TGGGCAACCT AATGGGAATG GAGAAGCAGT ACCAGATGAA CTATAAACCC 
ATGCCCAACA TGGCTCAGGG GCAGATGCTC CGTCATCAGC TACAGGTCAG ACTGGGTCAT 
AATCATTTAA TTGGACAGCA GATTAGACAA GTGACACCTA ATCCACAGTA TTCATCAATG 
CAGCCAGCAC AGAATATACC CCAGGGCTAC ACCTCCTACG GCTCACACAT GGGGATGCAG 
CCACACCCCT CTCAGACCCC TGGAATGGTT CCCAATTCAT ATGGAAACCA GGGCTTTCAG 
ACAGGACATC CTGCCACTAA CCCAACTATG GTGGACTCAA TAAGGCAGAT GCAGCAGAGA 
CCTGGCTACG TCCATCAGCA GGCTCCTGCG GCATATGGGC ACACAATCCA GAGCACACAA 
AGGTTTCCGC ATCAATCCAT GCAGCAGACA CCCATGATGC ACGGCTTGGC TCAAAGTCAC 
TTGGGAGTGC AGGGCATCCA CCCAAACATG AGGCCCAATC AGATGATGGA GCAGCAGCAG 
CAACAACAAC AACAACAACA GCAACAGCAG CAACAACAAC AACAACAACA GGTCCACCAG 
CAGCAGCAGC ACATCCGACA GGGTCCAGTT TTTAGACCAC AGCAGCAACA GCAGGTTCAG 
CAGCAAGTGC AACAGCAGGT CCAGCAGCAG CAGGTCCAAC AGCAGCAGGT CCAGCAACAG 
GTTCAGCCTC AGCAAGTTCA ACAGCAGCAG GTTCAGCAGC AGCAGCAGCA GGTGTCTGCA 
GCTCAGCCTC CCGCTCAGGC CCTGGGCATG CAGGCTCTGC CCCCGCAACA GCCCATGTTC 
CAGCGGCAAG GCATGCAGCA AACCCAGCAG CAGCAGCAGA CCGCAGCACT GGTCAGGCAA 
CTCCAGCAGC AGCTCTCCAG TACTCAACCT GCACAGAACA ACAGCTCCTA TTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

MED12 ( XM_009291039.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009289314.1 Danio rerio mediator complex subunit 12 (med12), transcript variant X3, mRNA.

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CloneID	ODa44931
Clone ID Related Accession (Same CDS sequence)	XM_009291039.2
Accession Version	XM_009291039.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6531bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	mediator of RNA polymerase II transcription subunit 12 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007125.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291039.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGCAGATGTT TTCTCCTCCA ATGCACTGTG AGTCGAAGGG AAGCCCTTCT 
CCAGAGAAAC AAGCCCAAGA GCAGGAGAGC AAAAGCACTG CCAAGGATAA GGGCATGGAC 
CCAGCCTTCC CTTTGGTTTA TGAACAGCCC CGCCACATAC AGTACGCCAC CCACTTCCCC 
ATTCCTCAGG AGGAGAGTGC AAGTCATGAG TGTAACCAGC GTCTAGTGGT TCTCTATGGA 
GTCGGCAAGC AGCGAGATGA AGCTCGCCAC GCCATCAAGA AGATCACCAA AGACATCCTG 
AAGGTTCTCA ACCGCAAGAG TACAGCAGAG ACAGGAGGTG AGGAAGGCCA GAAGAGGAAG 
AGGAGCAAAC CTGAGGCCTT CCCCACTGCT GAAGATATTT TCTCTAAATT CCAGCACCTC 
TCACACTTTG ACCAGCACCA GGTCACCTCA CAGGTGTCCA GGAATGTTCT GGAGCAAATC 
ACCAGCTTTG CCTTAGGGAT GTCCTATCAC CTCCCACTGG TCCAACACAT CCAGTTCATC 
TTTGACCTCA TGGAGTACTC TCTTAATATA AGTGGCCTCA TTGACTTTGC TATACAGCTG 
CTGAATGAAT TGAGTCTGGT GGAAGCAGAA CTCCTGCTTA AGTCCTCCAA CCTGGCGGGC 
AGTTACACTA CAGGCCTCTG TTTGTGTATC GTGGCTGTTC TGCGGAGATA CCACTCCTGC 
CTCATCCTCA ACCCTGACCA GACCGCACAG GTCTTTGACG GGTTGCGGAT TGTAGTCAAG 
TCTGGTGTGA ATCCTGCAGA CTGTTCCTCA GCAGAACGCT GTATCCTAGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTAAAG AGCAAGTTTG GAGAGATTTT CAGTGAGTTC 
TGCTCAAAGG TGAAGAATTC CATCTATTAT AACATCGACC CGTCAGACTC CAACATGCTG 
TGGGATCAGA TGTTTATGAT CGATGCCATC ACTAATCCTA CCGCACACAA CCTCAACCAC 
TCCATGTTGG GAAAGATCAC CAATGAACTC AATGACAGCC CGGCTAACCG CTACAGCTTT 
GTGTGTAATG TGCTCATGGA TGTTTGCGTT GACCATCGAG ATCCTGAGAG GGTGAATGAT 
ATTGGTATCC TGTGTGCGGA GCTGACAGCA TACTGTCGCT CTCTAAGTGC TGAGTGGTTG 
GGTGTGCTCA AGGCCCTCTG CTGCTCTTCT AACAACGGCA ACTGTGGCTT CAACGATCTG 
CTCTGCAATG TTGACGTGAG CGATCTATCT TTCCACGACT CTCTAGCAAC ATTCGTTGCC 
ATCTTAATAG CTCGGCAGTG TTTGTTGCTG GAAGATCTGG TGCGCTGTGT TGCCATCCCA 
TCCCTCCTGA ATGCAGCCTG TAGTGAACAG GATTCTGAAC CTGGAGCCAG ACTGACATGT 
AGGATCCTGC TTCATCTGTT CAGAACCCCA CAGCGCAACC CCTGTCCCCA GGATGGCACT 
AAATCAGACA AATCCATAGT GGGGATCAGG TCGTCCTGCG ACCGACATCT GCTGGCTGCT 
TCACAGAACA GCATCGTGGT GGGAGCCGTT TTTGCTGTAT TAAAAGCTGT GTTCATGCTG 
GGAGATGCTG AGCTGAAAGG CTCAGGCTTT TCCCATCCTG CTGGTCTGGA TGATATTGGA 
GAGGATGACA TGGGCTCTAA AAAATCTGGA GGACGTAATG TCTCTATTGA AACAGCCAGT 
CTGGTGGTTT ACGCCAAGTA TGTGCTGAAG AGCATCTGCC ACCAGGAATG GGTTGGCGAG 
CGCTGTCTGA AGTCTCTGTC AGAAGACAGC AGTGCACTGC AGGATCCAGT GCTGGTCAAC 
ATCCAGGCTC AGCGCTTGCT GCAGCTCATC TGTTACCCAC ACCGACAGCT GGACAGTGAA 
GAGGGTGACA ATCCACAGAG ACAGCGCATC AAACGCATCC TGCAGAACAT GGACCAGTGG 
ACCATGAGAC AGTCCTCTCT TGAGCTACAA CTCATGATCA AGCAAAGCTC AAATAATGAG 
CTGTATTCTC TTCTGGAGAA CATAGCCAAG GCCACTATTG AGGTTTTCCA GAAGTCTGCA 
GAGATGAACT CTAGTAACCC CTCCTGGAAT GGCTCTGCTG TCTCGGGTAG CTCAGTCTCC 
AATAGCAACA GTGCCAGCAA ACTCAAACCT GTGCTAAGTT CCTCTGAGCG TTCAGGTGTC 
TGGTTAGTTG CCCCTCTAAT TGGTAAGCTG CCCACCTCAG TGCAGGGGCA CGTGCTCAAG 
GCTGCAGGCG AGGAGCTGGA GAAGGGACAG CATCTGGGAC CCTCTTCACG AAAAGAAAGA 
GATCGACAGA AGCAAAAAAG CATGTCTCTC CTGAGCCAGC AGCCCTTCCT GTCACTGGTT 
CTGACATGTC TCAAAGGGCA AGACGAGCAG AGAGAGGGTC TCTTGACGTC CCTCTACAGC 
CAAGTCCAGC AGATTGTGAC TAACTGGCGA GAGGACCAGT ACCAGGACGA CTGCAAGGCT 
AAGCAGATGA TGCACGAGGC CTTGAAGCTG CGACTCAATC TGGTGGGTGG GATGTTTGAC 
ACTGTACAGC GTAGCACTCA GCAGACAACA GAGTGGGCAG TGCTTCTGCT GGACATCATC 
AGCAGTGGCA CAGTGGACAT GCAGTCTAAC AATGAGCTCT TCACTACGGT TTTAGACATG 
CTGAGCGTGT TGATTAACGG GACTCTGGCG GCTGACATGT CCAGCATATC TCAGGGCAGC 
ATGGAGGAGA ATAAGAGAGC TTACATGAAC CTGGTCAAGA AGCTCAGGAA AGAACTTGGT 
GACCGACAAT CCGAGAGCTT GGAGAAAGTG CGTCAGTTGC TGCCTCTGCC CAAGCAGACG 
CGTGATGTCA TCACCTGTGA GCCCCAGGGG TCACTCATTG ACACAAAGGG CAACAAAATT 
GCTGGATTTG AAAAAGAGGG TCTTCAGGTA TCAACTAAGC AGAAGATTTC TCCATGGGAT 
GTGTTTGAGG GACTAAAGCA CTCTGCTCCG CTCTCCTGGG GCTGGTTCGG AACCATACGG 
GTCGACCGCA AAGTCACTAA GTTTGAAGAG CAGCAGCGGC TCCTGCTTTA CCACACACAC 
ATCAAGCCCA AGCAACGCAG TTACTACCTG GCGCCCCTCA ACCTGCCCCA AGAAGAAGAG 
GAACCACTCA CTCCTGTGCC TCCCGAGCCA GACAAGAAGC TGGACACAGC GAAGGTGGAG 
AAAAGCGTCT CCAATGTCCC GACTAAGAAA AAGAAAAAGA AACCTGCATC GACGGTTAAA 
CAAGAGGACT ACAAGCCACA CAACACAGTC ATGCAGTACG CTCCAGGCAT GACCCCTGAT 
CTCCCACTGA GCATGGGACA AACAAACATG TCATATCGAA TGGGCTACCA AGCTCCAATG 
AACATGTATG CCCAGAACCA GCCTTTACCA CCAGGAGGTC CTGGTTTGGA GCCACCTTTT 
CGTCCTAGGG TTCCTATGAA TAAAATGCCT CCACGTCCCA ACTACACTAT GCCTAATATG 
CAGACCGCTG GCATGGGCAA CCTAATGGGA ATGGAGAAGC AGTACCAGAT GAACTATAAA 
CCCATGCCCA ACATGGCTCA GGGGCAGATG CTCCGTCATC AGCTACAGGG TCATAATCAT 
TTAATTGGAC AGCAGATTAG ACAAGTGACA CCTAATCCAC AGTATTCATC AATGCAGCCA 
GCACAGAATA TACCCCAGGG CTACACCTCC TACGGCTCAC ACATGGGGAT GCAGCCACAC 
CCCTCTCAGA CCCCTGGAAT GGTTCCCAAT TCATATGGAA ACCAGGGCTT TCAGACAGGA 
CATCCTGCCA CTAACCCAAC TATGGTGGAC TCAATAAGGC AGATGCAGCA GAGACCTGGC 
TACGTCCATC AGCAGGCTCC TGCGGCATAT GGGCACACAA TCCAGAGCAC ACAAAGGTTT 
CCGCATCAAT CCATGCAGCA GACACCCATG ATGCACGGCT TGGCTCAAAG TCACTTGGGA 
GTGCAGGGCA TCCACCCAAA CATGAGGCCC AATCAGATGA TGGAGCAGCA GCAGCAACAA 
CAACAACAAC AACAGCAACA GCAGCAACAA CAACAACAAC AACAGGTCCA CCAGCAGCAG 
CAGCACATCC GACAGGGTCC AGTTTTTAGA CCACAGCAGC AACAGCAGGT TCAGCAGCAA 
GTGCAACAGC AGGTCCAGCA GCAGCAGGTC CAACAGCAGC AGGTCCAGCA ACAGGTTCAG 
CCTCAGCAAG TTCAACAGCA GCAGGTTCAG CAGCAGCAGC AGCAGGTGTC TGCAGCTCAG 
CCTCCCGCTC AGGCCCTGGG CATGCAGGCT CTGCCCCCGC AACAGCCCAT GTTCCAGCGG 
CAAGGCATGC AGCAAACCCA GCAGCAGCAG CAGACCGCAG CACTGGTCAG GCAACTCCAG 
CAGCAGCTCT CCAGTACTCA ACCTGCACAG AACAACAGCT CCTATTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009289314.1
CDS	92..6622
Translation	MMAAFGVLSYEHRPLKRPRLGPPDVYPQDPKQKEDELTALNVKQGFNNQPAVSGDEHGSAKNVNFNSSKISSNFSSIIAEKLRCNTFPDTGKRKPQVNQKDNFWLVTARSQSSINNWFTDLAGTKPLTQLAKKVPIFSKKEEVFGYLAKYSVPVMRSAWMIKMTCAYHAAITENKMKKRHVIDPCIEWTQIITKYLWEQLQKVAEFYRQSPSQGCGSPLPAPPAEVETAMKQWEYNEKLAMFMFQDGMLDRHEFLTWVLECFEKIRPGEDELLRLLLPLLLQYSGEFVQSAYLSRRLAYFCTRRLNLLLSDGSIGPGPGGHQAHGISAQQGNALPPTPTSQPAGGNQPQTPFTDFYICPQHRPVVFGLSCMLQSIVLCCPSALVWHYSLTDSRNKTGSPLDLLPIAPSNLPMPGGNSTFNQQVRAKVREIEEQIKERGQAVEFRWSFDKCQETTAGFTISRVLHTLEVLDNHSFEKSDFSNSLDSLYNRIFGSGQSKDGHEMSPDDDAVVTLLCEWAVSCKRSGPHRAMVVAKLLEKRQTEIEAERCGESEVVDEKGSVSSGSLSAATLPVFQDVLLQFLDTQAPVLTEPGNESERVEFSNLVLLFYELIRHDVFSHNIYMCTLISRGDLASNSHLPRPRSPSDEPSDESERKDQDAGSSVKIEDTGMSESMEIDHNSSANFDEQMFSPPMHCESKGSPSPEKQAQEQESKSTAKDKGMDPAFPLVYEQPRHIQYATHFPIPQEESASHECNQRLVVLYGVGKQRDEARHAIKKITKDILKVLNRKSTAETGGEEGQKRKRSKPEAFPTAEDIFSKFQHLSHFDQHQVTSQVSRNVLEQITSFALGMSYHLPLVQHIQFIFDLMEYSLNISGLIDFAIQLLNELSLVEAELLLKSSNLAGSYTTGLCLCIVAVLRRYHSCLILNPDQTAQVFDGLRIVVKSGVNPADCSSAERCILAYLYDLYTSCSHLKSKFGEIFSEFCSKVKNSIYYNIDPSDSNMLWDQMFMIDAITNPTAHNLNHSMLGKITNELNDSPANRYSFVCNVLMDVCVDHRDPERVNDIGILCAELTAYCRSLSAEWLGVLKALCCSSNNGNCGFNDLLCNVDVSDLSFHDSLATFVAILIARQCLLLEDLVRCVAIPSLLNAACSEQDSEPGARLTCRILLHLFRTPQRNPCPQDGTKSDKSIVGIRSSCDRHLLAASQNSIVVGAVFAVLKAVFMLGDAELKGSGFSHPAGLDDIGEDDMGSKKSGGRNVSIETASLVVYAKYVLKSICHQEWVGERCLKSLSEDSSALQDPVLVNIQAQRLLQLICYPHRQLDSEEGDNPQRQRIKRILQNMDQWTMRQSSLELQLMIKQSSNNELYSLLENIAKATIEVFQKSAEMNSSNPSWNGSAVSGSSVSNSNSASKLKPVLSSSERSGVWLVAPLIGKLPTSVQGHVLKAAGEELEKGQHLGPSSRKERDRQKQKSMSLLSQQPFLSLVLTCLKGQDEQREGLLTSLYSQVQQIVTNWREDQYQDDCKAKQMMHEALKLRLNLVGGMFDTVQRSTQQTTEWAVLLLDIISSGTVDMQSNNELFTTVLDMLSVLINGTLAADMSSISQGSMEENKRAYMNLVKKLRKELGDRQSESLEKVRQLLPLPKQTRDVITCEPQGSLIDTKGNKIAGFEKEGLQVSTKQKISPWDVFEGLKHSAPLSWGWFGTIRVDRKVTKFEEQQRLLLYHTHIKPKQRSYYLAPLNLPQEEEEPLTPVPPEPDKKLDTAKVEKSVSNVPTKKKKKKPASTVKQEDYKPHNTVMQYAPGMTPDLPLSMGQTNMSYRMGYQAPMNMYAQNQPLPPGGPGLEPPFRPRVPMNKMPPRPNYTMPNMQTAGMGNLMGMEKQYQMNYKPMPNMAQGQMLRHQLQGHNHLIGQQIRQVTPNPQYSSMQPAQNIPQGYTSYGSHMGMQPHPSQTPGMVPNSYGNQGFQTGHPATNPTMVDSIRQMQQRPGYVHQQAPAAYGHTIQSTQRFPHQSMQQTPMMHGLAQSHLGVQGIHPNMRPNQMMEQQQQQQQQQQQQQQQQQQQQVHQQQQHIRQGPVFRPQQQQQVQQQVQQQVQQQQVQQQQVQQQVQPQQVQQQQVQQQQQQVSAAQPPAQALGMQALPPQQPMFQRQGMQQTQQQQQTAALVRQLQQQLSSTQPAQNNSSYY

Target ORF information:

RefSeq Version	XM_009291039.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mediator complex subunit 12 (med12), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009291039.2

ORF Insert Sequence:

ATGATGGCTG CCTTCGGCGT TCTGAGCTAC GAACACAGGC CTCTTAAACG GCCAAGACTC 
GGTCCTCCGG ACGTTTATCC ACAGGACCCG AAGCAAAAAG AGGATGAGTT AACCGCATTG 
AACGTCAAGC AAGGATTTAA CAACCAGCCA GCTGTTTCAG GCGATGAACA TGGCAGCGCT 
AAAAATGTCA ATTTCAACTC TTCAAAGATC AGCTCAAACT TCAGCAGCAT CATTGCAGAG 
AAGTTACGCT GCAACACTTT TCCAGACACA GGGAAGAGGA AGCCGCAGGT CAACCAGAAG 
GATAATTTCT GGCTCGTCAC AGCGAGGTCA CAGAGCTCCA TCAATAACTG GTTTACAGAC 
TTGGCCGGGA CCAAACCCCT AACTCAGCTC GCTAAAAAGG TTCCCATCTT CAGCAAGAAA 
GAGGAAGTGT TTGGCTACCT TGCCAAGTAC TCTGTGCCAG TGATGCGATC TGCTTGGATG 
ATTAAAATGA CATGTGCGTA TCACGCTGCC ATAACTGAAA ACAAGATGAA GAAGAGGCAC 
GTCATCGACC CCTGTATAGA ATGGACTCAG ATAATTACTA AATACCTGTG GGAACAGCTG 
CAGAAGGTGG CGGAGTTTTA CAGACAGTCT CCCAGCCAAG GCTGCGGGTC TCCTCTGCCT 
GCTCCTCCTG CCGAGGTGGA AACTGCCATG AAACAGTGGG AGTACAACGA GAAACTGGCC 
ATGTTCATGT TCCAGGATGG TATGTTAGAC AGACACGAGT TCCTCACCTG GGTGCTGGAG 
TGCTTTGAGA AGATTAGACC TGGAGAGGAT GAGCTTCTAC GTTTGCTTCT GCCACTTCTG 
TTACAGTACT CTGGGGAGTT TGTGCAGTCA GCATATTTAT CTAGACGCTT GGCGTATTTC 
TGCACACGCC GGCTCAATTT GCTGCTGAGC GATGGCAGTA TCGGGCCTGG TCCAGGTGGG 
CATCAGGCCC ATGGCATTTC TGCGCAGCAG GGGAACGCTC TACCCCCAAC ACCCACATCT 
CAACCTGCAG GGGGCAACCA GCCACAGACC CCCTTCACGG ACTTCTACAT CTGCCCTCAG 
CATAGGCCAG TGGTGTTTGG ACTAAGCTGC ATGCTACAGA GTATTGTGTT GTGTTGCCCC 
AGCGCTCTGG TCTGGCACTA CTCTCTGACA GATAGTCGTA ATAAGACTGG CTCTCCATTA 
GACCTCTTGC CAATCGCCCC CTCTAATCTC CCTATGCCAG GGGGCAACAG CACCTTTAAC 
CAACAGGTTC GGGCAAAGGT CCGGGAGATT GAAGAGCAGA TTAAAGAGAG AGGGCAGGCC 
GTGGAATTTC GCTGGTCCTT TGACAAGTGT CAGGAGACAA CAGCAGGTTT CACCATTAGC 
CGGGTTTTGC ATACATTGGA GGTTTTGGAT AACCACAGCT TTGAGAAGTC TGACTTCAGC 
AACTCTTTGG ATTCTCTATA CAATCGGATC TTTGGCTCAG GACAAAGCAA AGATGGGCAT 
GAGATGAGTC CAGACGATGA TGCAGTGGTC ACCCTGCTGT GTGAGTGGGC TGTTTCTTGT 
AAAAGGTCAG GGCCACATAG AGCCATGGTG GTGGCCAAAC TCCTGGAGAA AAGGCAGACT 
GAGATTGAAG CAGAGAGGTG CGGCGAGTCA GAAGTTGTAG ATGAGAAAGG CTCTGTGTCC 
TCAGGGTCTC TGTCTGCTGC CACACTCCCT GTCTTTCAGG ATGTCCTGCT GCAGTTTCTG 
GACACACAGG CCCCTGTGCT CACTGAGCCT GGGAACGAGA GTGAGCGAGT GGAGTTCTCT 
AACCTTGTGC TCCTGTTCTA CGAGCTCATC CGTCATGATG TCTTCTCACA CAACATCTAC 
ATGTGCACCC TTATATCCCG TGGGGACCTG GCCTCCAACT CTCATCTGCC CCGCCCTCGT 
TCCCCCAGCG ATGAGCCCTC TGATGAGTCA GAGCGCAAGG ACCAGGATGC AGGAAGTAGT 
GTTAAAATCG AGGATACTGG TATGTCCGAG TCGATGGAGA TAGATCACAA CTCAAGCGCT 
AACTTTGATG AGCAGATGTT TTCTCCTCCA ATGCACTGTG AGTCGAAGGG AAGCCCTTCT 
CCAGAGAAAC AAGCCCAAGA GCAGGAGAGC AAAAGCACTG CCAAGGATAA GGGCATGGAC 
CCAGCCTTCC CTTTGGTTTA TGAACAGCCC CGCCACATAC AGTACGCCAC CCACTTCCCC 
ATTCCTCAGG AGGAGAGTGC AAGTCATGAG TGTAACCAGC GTCTAGTGGT TCTCTATGGA 
GTCGGCAAGC AGCGAGATGA AGCTCGCCAC GCCATCAAGA AGATCACCAA AGACATCCTG 
AAGGTTCTCA ACCGCAAGAG TACAGCAGAG ACAGGAGGTG AGGAAGGCCA GAAGAGGAAG 
AGGAGCAAAC CTGAGGCCTT CCCCACTGCT GAAGATATTT TCTCTAAATT CCAGCACCTC 
TCACACTTTG ACCAGCACCA GGTCACCTCA CAGGTGTCCA GGAATGTTCT GGAGCAAATC 
ACCAGCTTTG CCTTAGGGAT GTCCTATCAC CTCCCACTGG TCCAACACAT CCAGTTCATC 
TTTGACCTCA TGGAGTACTC TCTTAATATA AGTGGCCTCA TTGACTTTGC TATACAGCTG 
CTGAATGAAT TGAGTCTGGT GGAAGCAGAA CTCCTGCTTA AGTCCTCCAA CCTGGCGGGC 
AGTTACACTA CAGGCCTCTG TTTGTGTATC GTGGCTGTTC TGCGGAGATA CCACTCCTGC 
CTCATCCTCA ACCCTGACCA GACCGCACAG GTCTTTGACG GGTTGCGGAT TGTAGTCAAG 
TCTGGTGTGA ATCCTGCAGA CTGTTCCTCA GCAGAACGCT GTATCCTAGC CTATCTCTAC 
GACCTCTATA CCTCCTGCAG TCACCTAAAG AGCAAGTTTG GAGAGATTTT CAGTGAGTTC 
TGCTCAAAGG TGAAGAATTC CATCTATTAT AACATCGACC CGTCAGACTC CAACATGCTG 
TGGGATCAGA TGTTTATGAT CGATGCCATC ACTAATCCTA CCGCACACAA CCTCAACCAC 
TCCATGTTGG GAAAGATCAC CAATGAACTC AATGACAGCC CGGCTAACCG CTACAGCTTT 
GTGTGTAATG TGCTCATGGA TGTTTGCGTT GACCATCGAG ATCCTGAGAG GGTGAATGAT 
ATTGGTATCC TGTGTGCGGA GCTGACAGCA TACTGTCGCT CTCTAAGTGC TGAGTGGTTG 
GGTGTGCTCA AGGCCCTCTG CTGCTCTTCT AACAACGGCA ACTGTGGCTT CAACGATCTG 
CTCTGCAATG TTGACGTGAG CGATCTATCT TTCCACGACT CTCTAGCAAC ATTCGTTGCC 
ATCTTAATAG CTCGGCAGTG TTTGTTGCTG GAAGATCTGG TGCGCTGTGT TGCCATCCCA 
TCCCTCCTGA ATGCAGCCTG TAGTGAACAG GATTCTGAAC CTGGAGCCAG ACTGACATGT 
AGGATCCTGC TTCATCTGTT CAGAACCCCA CAGCGCAACC CCTGTCCCCA GGATGGCACT 
AAATCAGACA AATCCATAGT GGGGATCAGG TCGTCCTGCG ACCGACATCT GCTGGCTGCT 
TCACAGAACA GCATCGTGGT GGGAGCCGTT TTTGCTGTAT TAAAAGCTGT GTTCATGCTG 
GGAGATGCTG AGCTGAAAGG CTCAGGCTTT TCCCATCCTG CTGGTCTGGA TGATATTGGA 
GAGGATGACA TGGGCTCTAA AAAATCTGGA GGACGTAATG TCTCTATTGA AACAGCCAGT 
CTGGTGGTTT ACGCCAAGTA TGTGCTGAAG AGCATCTGCC ACCAGGAATG GGTTGGCGAG 
CGCTGTCTGA AGTCTCTGTC AGAAGACAGC AGTGCACTGC AGGATCCAGT GCTGGTCAAC 
ATCCAGGCTC AGCGCTTGCT GCAGCTCATC TGTTACCCAC ACCGACAGCT GGACAGTGAA 
GAGGGTGACA ATCCACAGAG ACAGCGCATC AAACGCATCC TGCAGAACAT GGACCAGTGG 
ACCATGAGAC AGTCCTCTCT TGAGCTACAA CTCATGATCA AGCAAAGCTC AAATAATGAG 
CTGTATTCTC TTCTGGAGAA CATAGCCAAG GCCACTATTG AGGTTTTCCA GAAGTCTGCA 
GAGATGAACT CTAGTAACCC CTCCTGGAAT GGCTCTGCTG TCTCGGGTAG CTCAGTCTCC 
AATAGCAACA GTGCCAGCAA ACTCAAACCT GTGCTAAGTT CCTCTGAGCG TTCAGGTGTC 
TGGTTAGTTG CCCCTCTAAT TGGTAAGCTG CCCACCTCAG TGCAGGGGCA CGTGCTCAAG 
GCTGCAGGCG AGGAGCTGGA GAAGGGACAG CATCTGGGAC CCTCTTCACG AAAAGAAAGA 
GATCGACAGA AGCAAAAAAG CATGTCTCTC CTGAGCCAGC AGCCCTTCCT GTCACTGGTT 
CTGACATGTC TCAAAGGGCA AGACGAGCAG AGAGAGGGTC TCTTGACGTC CCTCTACAGC 
CAAGTCCAGC AGATTGTGAC TAACTGGCGA GAGGACCAGT ACCAGGACGA CTGCAAGGCT 
AAGCAGATGA TGCACGAGGC CTTGAAGCTG CGACTCAATC TGGTGGGTGG GATGTTTGAC 
ACTGTACAGC GTAGCACTCA GCAGACAACA GAGTGGGCAG TGCTTCTGCT GGACATCATC 
AGCAGTGGCA CAGTGGACAT GCAGTCTAAC AATGAGCTCT TCACTACGGT TTTAGACATG 
CTGAGCGTGT TGATTAACGG GACTCTGGCG GCTGACATGT CCAGCATATC TCAGGGCAGC 
ATGGAGGAGA ATAAGAGAGC TTACATGAAC CTGGTCAAGA AGCTCAGGAA AGAACTTGGT 
GACCGACAAT CCGAGAGCTT GGAGAAAGTG CGTCAGTTGC TGCCTCTGCC CAAGCAGACG 
CGTGATGTCA TCACCTGTGA GCCCCAGGGG TCACTCATTG ACACAAAGGG CAACAAAATT 
GCTGGATTTG AAAAAGAGGG TCTTCAGGTA TCAACTAAGC AGAAGATTTC TCCATGGGAT 
GTGTTTGAGG GACTAAAGCA CTCTGCTCCG CTCTCCTGGG GCTGGTTCGG AACCATACGG 
GTCGACCGCA AAGTCACTAA GTTTGAAGAG CAGCAGCGGC TCCTGCTTTA CCACACACAC 
ATCAAGCCCA AGCAACGCAG TTACTACCTG GCGCCCCTCA ACCTGCCCCA AGAAGAAGAG 
GAACCACTCA CTCCTGTGCC TCCCGAGCCA GACAAGAAGC TGGACACAGC GAAGGTGGAG 
AAAAGCGTCT CCAATGTCCC GACTAAGAAA AAGAAAAAGA AACCTGCATC GACGGTTAAA 
CAAGAGGACT ACAAGCCACA CAACACAGTC ATGCAGTACG CTCCAGGCAT GACCCCTGAT 
CTCCCACTGA GCATGGGACA AACAAACATG TCATATCGAA TGGGCTACCA AGCTCCAATG 
AACATGTATG CCCAGAACCA GCCTTTACCA CCAGGAGGTC CTGGTTTGGA GCCACCTTTT 
CGTCCTAGGG TTCCTATGAA TAAAATGCCT CCACGTCCCA ACTACACTAT GCCTAATATG 
CAGACCGCTG GCATGGGCAA CCTAATGGGA ATGGAGAAGC AGTACCAGAT GAACTATAAA 
CCCATGCCCA ACATGGCTCA GGGGCAGATG CTCCGTCATC AGCTACAGGG TCATAATCAT 
TTAATTGGAC AGCAGATTAG ACAAGTGACA CCTAATCCAC AGTATTCATC AATGCAGCCA 
GCACAGAATA TACCCCAGGG CTACACCTCC TACGGCTCAC ACATGGGGAT GCAGCCACAC 
CCCTCTCAGA CCCCTGGAAT GGTTCCCAAT TCATATGGAA ACCAGGGCTT TCAGACAGGA 
CATCCTGCCA CTAACCCAAC TATGGTGGAC TCAATAAGGC AGATGCAGCA GAGACCTGGC 
TACGTCCATC AGCAGGCTCC TGCGGCATAT GGGCACACAA TCCAGAGCAC ACAAAGGTTT 
CCGCATCAAT CCATGCAGCA GACACCCATG ATGCACGGCT TGGCTCAAAG TCACTTGGGA 
GTGCAGGGCA TCCACCCAAA CATGAGGCCC AATCAGATGA TGGAGCAGCA GCAGCAACAA 
CAACAACAAC AACAGCAACA GCAGCAACAA CAACAACAAC AACAGGTCCA CCAGCAGCAG 
CAGCACATCC GACAGGGTCC AGTTTTTAGA CCACAGCAGC AACAGCAGGT TCAGCAGCAA 
GTGCAACAGC AGGTCCAGCA GCAGCAGGTC CAACAGCAGC AGGTCCAGCA ACAGGTTCAG 
CCTCAGCAAG TTCAACAGCA GCAGGTTCAG CAGCAGCAGC AGCAGGTGTC TGCAGCTCAG 
CCTCCCGCTC AGGCCCTGGG CATGCAGGCT CTGCCCCCGC AACAGCCCAT GTTCCAGCGG 
CAAGGCATGC AGCAAACCCA GCAGCAGCAG CAGACCGCAG CACTGGTCAG GCAACTCCAG 
CAGCAGCTCT CCAGTACTCA ACCTGCACAG AACAACAGCT CCTATTACTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Nipbl and mediator cooperatively regulate gene expression to control limb development.
PLoS genetics10(9)e1004671(2014 Sep)
Muto A,Ikeda S,Lopez-Burks ME,Kikuchi Y,Calof AL,Lander AD,Schilling TF

Mediator subunit 12 coordinates intrinsic and extrinsic control of epithalamic development.
Developmental biology385(1)13-22(2014 Jan)
Wu SY,de Borsetti NH,Bain EJ,Bulow CR,Gamse JT

Mediator subunit 12 is required for neutrophil development in zebrafish.
PloS one6(8)e23845(2011)
Keightley MC,Layton JE,Hayman JW,Heath JK,Lieschke GJ

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MED12 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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