tns2b cDNA ORF clone, Danio rerio(zebrafish)

The following tns2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tns2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa144741 XM_021471813.1
Latest version!
Danio rerio tensin 2b (tns2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa144742 XM_021471814.1
Latest version!
Danio rerio tensin 2b (tns2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa12095 XM_689077.9
Latest version!
Danio rerio tensin 2b (tns2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa12096 XM_005166088.4
Latest version!
Danio rerio tensin 2b (tns2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa44724 XM_009302575.2
Latest version!
Danio rerio tensin 2b (tns2b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa12096 XM_005166088.3 Danio rerio tensin 2b (tns2b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa12095 XM_689077.8 Danio rerio tensin 2b (tns2b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa144741
    Clone ID Related Accession (Same CDS sequence) XM_021471813.1
    Accession Version XM_021471813.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394660.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CAAGAGACCA TACTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TATCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCGTTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGATGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCATGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGC CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    GTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTATGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGGAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATGTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT CCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTATC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTC
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACGGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACACCGCTCA
    TCGCCTCTAA ACAACACGTC TGATTATACT CCCACTTCCT CAATTAAATC AGCTGGAAAG
    TCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCTCCCTCT AGTGGTGTGG AGGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAAGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGTGGGTT GATGCTGATA ACACTTCCTG CAAGATGTTT
    GGATTTGTTG CACGGCGCAG TGGTGCTTTG GGAAACGTGT GTCACCTGTT TGCAGAGCTC
    GACCCGGAGC AACCCGCCAC GGCCATCGTG AACTTCATCA ATAAAGTCAT GCTGGGACCT
    CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327488.1
    CDS353..4690
    Translation

    Target ORF information:

    RefSeq Version XM_021471813.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 2b (tns2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471813.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CAAGAGACCA TACTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TATCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCGTTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGATGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCATGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGC CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    GTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTATGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGGAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATGTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT CCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTATC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTC
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACGGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACACCGCTCA
    TCGCCTCTAA ACAACACGTC TGATTATACT CCCACTTCCT CAATTAAATC AGCTGGAAAG
    TCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCTCCCTCT AGTGGTGTGG AGGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAAGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGTGGGTT GATGCTGATA ACACTTCCTG CAAGATGTTT
    GGATTTGTTG CACGGCGCAG TGGTGCTTTG GGAAACGTGT GTCACCTGTT TGCAGAGCTC
    GACCCGGAGC AACCCGCCAC GGCCATCGTG AACTTCATCA ATAAAGTCAT GCTGGGACCT
    CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa144742
    Clone ID Related Accession (Same CDS sequence) XM_021471814.1
    Accession Version XM_021471814.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018394660.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CAAGAGACCA TACTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TATCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCGTTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGATGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCATGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGC CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    GTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTATGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGGAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATGTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT CCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTATC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTC
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACGGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACACCGCTCA
    TCGCCTCTAA ACAACACGTC TGATTATACT CCCACTTCCT CAATTAAATC AGCTGGAAAG
    TCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCTCCCTCT AGTGGTGTGG AGGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAAGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGATGTTT GGATTTGTTG CACGGCGCAG TGGTGCTTTG
    GGAAACGTGT GTCACCTGTT TGCAGAGCTC GACCCGGAGC AACCCGCCAC GGCCATCGTG
    AACTTCATCA ATAAAGTCAT GCTGGGACCT CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021327489.1
    CDS354..4661
    Translation

    Target ORF information:

    RefSeq Version XM_021471814.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 2b (tns2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021471814.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CAAGAGACCA TACTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TATCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCGTTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGATGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCATGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGC CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    GTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTATGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGGAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATGTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT CCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTATC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTC
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACGGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACACCGCTCA
    TCGCCTCTAA ACAACACGTC TGATTATACT CCCACTTCCT CAATTAAATC AGCTGGAAAG
    TCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCTCCCTCT AGTGGTGTGG AGGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAAGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGATGTTT GGATTTGTTG CACGGCGCAG TGGTGCTTTG
    GGAAACGTGT GTCACCTGTT TGCAGAGCTC GACCCGGAGC AACCCGCCAC GGCCATCGTG
    AACTTCATCA ATAAAGTCAT GCTGGGACCT CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12095
    Clone ID Related Accession (Same CDS sequence) XM_689077.8 , XM_689077.9
    Accession Version XM_689077.8 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_689077.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGTGGGTT GATGCTGATA ACACTTCCTG CAAGATGTTT
    GGATTTGTTG CACGGCGCAG TGGTGCTTTG GGAAACGTGT GTCACCTGTT TGCAGAGCTC
    GACCCGGAGC AACCCGCCAC AGCCATCGTG AACTTCATCA ATAAAGTCAT GCTGGGACCT
    CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_694169.6
    CDS589..4926
    Translation

    Target ORF information:

    RefSeq Version XM_689077.8
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 2b (tns2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_689077.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGTGGGTT GATGCTGATA ACACTTCCTG CAAGATGTTT
    GGATTTGTTG CACGGCGCAG TGGTGCTTTG GGAAACGTGT GTCACCTGTT TGCAGAGCTC
    GACCCGGAGC AACCCGCCAC AGCCATCGTG AACTTCATCA ATAAAGTCAT GCTGGGACCT
    CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12095
    Clone ID Related Accession (Same CDS sequence) XM_689077.8 , XM_689077.9
    Accession Version XM_689077.9 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4338bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_689077.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGTGGGTT GATGCTGATA ACACTTCCTG CAAGATGTTT
    GGATTTGTTG CACGGCGCAG TGGTGCTTTG GGAAACGTGT GTCACCTGTT TGCAGAGCTC
    GACCCGGAGC AACCCGCCAC AGCCATCGTG AACTTCATCA ATAAAGTCAT GCTGGGACCT
    CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_694169.6
    CDS490..4827
    Translation

    Target ORF information:

    RefSeq Version XM_689077.9
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 2b (tns2b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_689077.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGTGGGTT GATGCTGATA ACACTTCCTG CAAGATGTTT
    GGATTTGTTG CACGGCGCAG TGGTGCTTTG GGAAACGTGT GTCACCTGTT TGCAGAGCTC
    GACCCGGAGC AACCCGCCAC AGCCATCGTG AACTTCATCA ATAAAGTCAT GCTGGGACCT
    CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12096
    Clone ID Related Accession (Same CDS sequence) XM_005166088.4 , XM_005166088.3
    Accession Version XM_005166088.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product tensin-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005166088.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGATGTTT GGATTTGTTG CACGGCGCAG TGGTGCTTTG
    GGAAACGTGT GTCACCTGTT TGCAGAGCTC GACCCGGAGC AACCCGCCAC AGCCATCGTG
    AACTTCATCA ATAAAGTCAT GCTGGGACCT CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166145.1
    CDS490..4797
    Translation

    Target ORF information:

    RefSeq Version XM_005166088.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio tensin 2b (tns2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166088.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGATGTTT GGATTTGTTG CACGGCGCAG TGGTGCTTTG
    GGAAACGTGT GTCACCTGTT TGCAGAGCTC GACCCGGAGC AACCCGCCAC AGCCATCGTG
    AACTTCATCA ATAAAGTCAT GCTGGGACCT CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa12096
    Clone ID Related Accession (Same CDS sequence) XM_005166088.4 , XM_005166088.3
    Accession Version XM_005166088.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4308bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005166088.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGATGTTT GGATTTGTTG CACGGCGCAG TGGTGCTTTG
    GGAAACGTGT GTCACCTGTT TGCAGAGCTC GACCCGGAGC AACCCGCCAC AGCCATCGTG
    AACTTCATCA ATAAAGTCAT GCTGGGACCT CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005166145.1
    CDS589..4896
    Translation

    Target ORF information:

    RefSeq Version XM_005166088.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 2b (tns2b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005166088.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    ATGGGGTGTA TCTTCAGCAA GCAGCAGTGG GAAAAAGAGA CAAGTTCTCA ACCCTCAGAC 
    CCCCCAAAAA CACGAGACCA TGCTGAGGAT GCAGAGATCC TCAGTTTGAC AGAGTTAGAG
    AAAATGGGTT CACACTGCTT TAAGGAGAGG AACTTCAAGA AGAAACTTCA CTGTGCTGTT
    TGCAGACAAA CACTCGACAG TCTCGGCATG TACTGCAGAG AATGTAAAAC TGCAGTGCAT
    AAGAAATGTG AGGAAAAGGT CTCTGTCCCC TGTGAAGCAG TTTCAGATCC TCCACCATTA
    CAAAACCAAA TGAATGTCCC TCAAAGTAAA GCCAGCATGG AGACCGTTAT GGAGAACCTG
    ATGGGCAGTC ATTATGATTT CGACTTGACC TACATCACTG AGCGAATCAT CTCTGTCCTG
    TATCAGTCTG ACCTGGACGA ACAGAGCTAC AGTGCTAACC TAAAAGTAGT GGCGCCCATG
    TTGAAATCTA AACATCAAGA CAAATTCTTG CTTATAAATC TGTCAGAGAA ACGTCATGAC
    ATCTGCAGGC TAAACCCAAA GGTTGAGGAG TTTGGGTGGC CTGATTTGCA TGCCCCGCCC
    CTCGATAAAA TATGTGCCGT ATGCAAGACT ATGGAGAACT GGCTTAACTC TGACCCCAAC
    AATGTGGTTG TGCTGCACTG CAAGGGAAAC AAAGGAAAAA CAGGGGTGAT TGTGGCAGCT
    TACATGCACT ATAGCAAGAT ATCAGCTGGG GCAGATCAGG CTCTCTCAAC AGTTGCTATG
    AGGAAATTTT GTGAGGATAA AATCTCTTCT TTTATCCTGC CATCCCAGAA CAGGTATATA
    TATTATTTTG CCGGTCTCCT CTCGGGCTCA ATAAAGATGA ACAGCTCACC TCTGTTCCTG
    CATCAGATTC ATGTTCCAGT TTCACCCAAC TACCAGTCAG GAGGAGGTTA TGCTCCCTTT
    CTGAAGATCT ATCAGTCGCT GCAGCTGGTC TATTCTTCAG GCAAATATGA TATTCAGGGC
    CCTAACAGTA AGACGCTGTG TGTAAATATT GAACCAGCTC TGCTGTTGAA GGGAGACATC
    CTGGTGAAGT GTTATCACCG CCTCTCAGAT GTGTGTAAGA GAGAATGTGT GTTCAGAGTC
    CAGTTTCACA CCTGTAGTGT TCATGGATCT CAGCTTGCGT TTGGCAAAGG GGAGCTGGAC
    CATGCCTGCA CAGATGATCG GTTTCCTTCA GATGCAACAG TGGAGTTGCT GTTTTCTACT
    GGCCCTCAAA GGAGAGGTGG AGATGTGCCA GGGAATGAGC CAGGAGTATC TGCGGATTAT
    GGCAGCACAG ATCCTGTTGT CCGCTGGGAC TCATATGAGA ACTTCAACCT TCACCACGAG
    GACAGTGTTG AGGATATCAG TCACACACGT GGACCTTTAG ATGGCAGTCT GTATGCTCAA
    GTGAAGAAGC GTCGAGCACA GGGCTCCAGT CCCAGCAGAT GCCCGACCTC CATCAAACCC
    TCCACATACT CACCCACTCA TTCCTCTTCT ACTTCTCCAA TCCATCTCTC CGTCCAGCCT
    CTCTCACTCA GCAAAGACAC AAGTCGCTCA TCTGCAGCCC CTGAATCCCT CACACCACTT
    CCCAGAGAAA AAAGAGAGAA AGATGAAAAC AGGGAAAGAC ACAGAGAAAC TGCGATACTT
    GATGATGGAG AATGCTCTCC ACTCAGACCT GATCGGCCTG TTCAGTCTTG CTATGGTCAC
    CCGTTTTCAC ACACTCACTC ATACTGTGGC CCAGATCTGA CTAGCCCACA TGCAATTGCA
    GTTCAACCCA AACATCGCAC GCTTCCCTGC AACCGCACAA CCCCATTGTC AGTCCGCGAC
    CTGTGTGTAT CCCAGCCAGA TCTTTTATGG GAGAGAGGGC GCTGTTTGCA CCATCCCTGC
    CCAGAATCTG TCAGACACCT CTACTCGTAT CCTCCACAAG AAACACACAC TCATTCGCCA
    ATCCCACAGA CTTCCCATTC CCACACACTT CCTCCACAAA CACGTGCGTA TTTCTCTGGG
    GAGGCGTGCC CTTTGTTTCA TTGCTCCGCC CTTCCTCAGG GCCACAACCA CATCCCTCCT
    TCTCTGTCCC CTAATCAGTC CCTACTCACC AGCCCTTACA GAGAGTTACG ATACAGCACA
    ACATCACCAA CTTCATGCTC TTGTCGGGAT TGTTTACGAT TACGAGAGGA TGTTGCCCTC
    CATTCTTTAC GAGGAAGGGA ATTAGAAACC CTTCCTTGGT CTAGAGAGGC GGAGTTTGGA
    ATCCGAAGGG AGGCACCTGT GCATTGGAGG GATGGGAGGG CGGAGTCTCA CTGGGAGGGA
    GTTCAAGAAG CTGAATACTG GCGTCGCAAA ATAGCCATGT CGCCAGTGAT GCTATCATAT
    GGTCACTGTC AAGCTGTCCC AAAACAAGAG CAAGCAGTAT ATGTCACAGA CCCCCAACCT
    GAACATGGAC TCAGTCCATT TCCCAGCCCC CAAAGCAGCG GTTACCACAG CCCTCATCCA
    CCCTGTCCCT GCTCTCCACA GCCGTTCAGA GACAGTCCAG GTTACGCCTC AATCAGTCAT
    TCCCAAAACT CCTCCCCCAT TGCTCTCACA CCATCTCCAA AACGAGCCAA TCACAATAGA
    GCCTCGGCTG AGGAGAACCA GCATGGCCCT AATGAGGAAT ATACTAAGAT TTTATCTGAT
    GATAAGAGTC CTGCAACCTT TTCTCCACCT ACCCAGAACA GTGGACCAGT TAAAGCAGGA
    GAAATGGTGC AAATCAAATC CCCAGAAGAT GTGAATCTCC CTGTGTCTCT GATCACCAAT
    ATCTCCTCTG CCAGTGGAGC AGAAGATAAG ACAACCATCA CAGATTGTAC ATCTAACAGT
    CTTTTAATTA GTAGCCAACC ATCGTCAAAG AATATGGAGT CAGTGCAGGT ACAAACGCTC
    AATCCCAGAC CTCTCAGTGT GCCAGAATCC GAGCAGGATA AAGGAGAGCG TCCCGGCTCA
    TGCTACACCT CAACTACAAC CTCAACAAGT CCTCAACCTC CACTTTGCTC CTCAGAAAGA
    AGCTCTGCTT CAGAATTCTC ATCATTTAAC TTAAACGGTG AAAGACAAAG CGTGACACCC
    TTGACTGACT GCAATGAGGA AAGTAACAGT GACCCTGATG GCTTCATCAC ACCCTCATTT
    CCTATAGCAT CACACGGCTA TCCTCTTCTG ACGGTGCCTC ATGTCCCCTA CACAGGCTAC
    ACTGAGATCA CCATACCGAC CAATTTAGCC CAACCTCCTC TTCCAGAAAA ACAGCGCTCA
    TCGCCTCTAC ACAACACGTC TGATTATACT CCCACTTCCT TAATTAAATC AGCTGGAAAG
    CCTTCGGTTT CCTCTGCACA GGTTCATGTG TCTTTCTCCA CTACAACAAA TGAAGTGCCT
    CCCAAATCTA CATGCAGAAT ACCCCTCTCT AGTGGTGTGG ACGAGCCCGA GAGCCGTCTC
    AGCTCCAAGT TTGTCCAGGA CAGCTCCAAA TACTGGTACA AACCTGGCAT CTCCCGGGAC
    CAGGCGATCG CAGTACTGAA GGATAAGGAG GCAGGCTGCT TCCTCATCAG GGATAGTAAT
    TCATTCCAGG GTGCGTATGG ATTGGCACTG AAAGTGAGTA CAGCTCCAGC CCACACCAAT
    TCCCACACAG AGCCGGGCAG CGCACAGGAA CAGCTGGTCA GACACTTCCT GATCGAGAGC
    GGCGCTAAAG GAGTCAAGAT CAAGGGCTGC CAAAATGAAA CATATTTCGG CTCTCTGTCT
    GCTTTGGTGT ATCAACACTC CATTACTCCT GTGTCTCTTC CTTGTGTCCT GCGTATTCCA
    GATCGAGATC TTGTTGGTGA ATTGCAAGAT TTGCAAAGTG GAACGAATAC CAGCACAGCA
    GCAGACCTGT TGAAACAGGG GGCAGCTTGT AACGTTCTCT ACCTGAACTC AGTGGAAACA
    GAGTCTCTGA CGGGCCCGCA GGCCGTCTCA AAAACAACCA AGTGCACTTT GACCCAGGAG
    CCTCGTCCTT CACCAACAGT CGTCCACTTC AAAGTGTCCA CTCAGGGCAT CACCCTCACT
    GATAACCAGC GCAGACTGTT TTTCAGGCGA CATTACCCAA TTCACAGTGT GACGTTCAGC
    AGTGTGGATC CACTGGATCA AAGGATGTTT GGATTTGTTG CACGGCGCAG TGGTGCTTTG
    GGAAACGTGT GTCACCTGTT TGCAGAGCTC GACCCGGAGC AACCCGCCAC AGCCATCGTG
    AACTTCATCA ATAAAGTCAT GCTGGGACCT CAACAGCTGC GAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa44724
    Clone ID Related Accession (Same CDS sequence) XM_009302575.2
    Accession Version XM_009302575.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4029bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product tensin-2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302575.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATGTCC CTCAAAGTAA AGCCAGCATG GAGACCGTTA TGGAGAACCT GATGGGCAGT 
    CATTATGATT TCGACTTGAC CTACATCACT GAGCGAATCA TCTCTGTCCT GTATCAGTCT
    GACCTGGACG AACAGAGCTA CAGTGCTAAC CTAAAAGTAG TGGCGCCCAT GTTGAAATCT
    AAACATCAAG ACAAATTCTT GCTTATAAAT CTGTCAGAGA AACGTCATGA CATCTGCAGG
    CTAAACCCAA AGGTTGAGGA GTTTGGGTGG CCTGATTTGC ATGCCCCGCC CCTCGATAAA
    ATATGTGCCG TATGCAAGAC TATGGAGAAC TGGCTTAACT CTGACCCCAA CAATGTGGTT
    GTGCTGCACT GCAAGGGAAA CAAAGGAAAA ACAGGGGTGA TTGTGGCAGC TTACATGCAC
    TATAGCAAGA TATCAGCTGG GGCAGATCAG GCTCTCTCAA CAGTTGCTAT GAGGAAATTT
    TGTGAGGATA AAATCTCTTC TTTTATCCTG CCATCCCAGA ACAGGTATAT ATATTATTTT
    GCCGGTCTCC TCTCGGGCTC AATAAAGATG AACAGCTCAC CTCTGTTCCT GCATCAGATT
    CATGTTCCAG TTTCACCCAA CTACCAGTCA GGAGGAGGTT ATGCTCCCTT TCTGAAGATC
    TATCAGTCGC TGCAGCTGGT CTATTCTTCA GGCAAATATG ATATTCAGGG CCCTAACAGT
    AAGACGCTGT GTGTAAATAT TGAACCAGCT CTGCTGTTGA AGGGAGACAT CCTGGTGAAG
    TGTTATCACC GCCTCTCAGA TGTGTGTAAG AGAGAATGTG TGTTCAGAGT CCAGTTTCAC
    ACCTGTAGTG TTCATGGATC TCAGCTTGCG TTTGGCAAAG GGGAGCTGGA CCATGCCTGC
    ACAGATGATC GGTTTCCTTC AGATGCAACA GTGGAGTTGC TGTTTTCTAC TGGCCCTCAA
    AGGAGAGGTG GAGATGTGCC AGGGAATGAG CCAGGAGTAT CTGCGGATTA TGGCAGCACA
    GATCCTGTTG TCCGCTGGGA CTCATATGAG AACTTCAACC TTCACCACGA GGACAGTGTT
    GAGGATATCA GTCACACACG TGGACCTTTA GATGGCAGTC TGTATGCTCA AGTGAAGAAG
    CGTCGAGCAC AGGGCTCCAG TCCCAGCAGA TGCCCGACCT CCATCAAACC CTCCACATAC
    TCACCCACTC ATTCCTCTTC TACTTCTCCA ATCCATCTCT CCGTCCAGCC TCTCTCACTC
    AGCAAAGACA CAAGTCGCTC ATCTGCAGCC CCTGAATCCC TCACACCACT TCCCAGAGAA
    AAAAGAGAGA AAGATGAAAA CAGGGAAAGA CACAGAGAAA CTGCGATACT TGATGATGGA
    GAATGCTCTC CACTCAGACC TGATCGGCCT GTTCAGTCTT GCTATGGTCA CCCGTTTTCA
    CACACTCACT CATACTGTGG CCCAGATCTG ACTAGCCCAC ATGCAATTGC AGTTCAACCC
    AAACATCGCA CGCTTCCCTG CAACCGCACA ACCCCATTGT CAGTCCGCGA CCTGTGTGTA
    TCCCAGCCAG ATCTTTTATG GGAGAGAGGG CGCTGTTTGC ACCATCCCTG CCCAGAATCT
    GTCAGACACC TCTACTCGTA TCCTCCACAA GAAACACACA CTCATTCGCC AATCCCACAG
    ACTTCCCATT CCCACACACT TCCTCCACAA ACACGTGCGT ATTTCTCTGG GGAGGCGTGC
    CCTTTGTTTC ATTGCTCCGC CCTTCCTCAG GGCCACAACC ACATCCCTCC TTCTCTGTCC
    CCTAATCAGT CCCTACTCAC CAGCCCTTAC AGAGAGTTAC GATACAGCAC AACATCACCA
    ACTTCATGCT CTTGTCGGGA TTGTTTACGA TTACGAGAGG ATGTTGCCCT CCATTCTTTA
    CGAGGAAGGG AATTAGAAAC CCTTCCTTGG TCTAGAGAGG CGGAGTTTGG AATCCGAAGG
    GAGGCACCTG TGCATTGGAG GGATGGGAGG GCGGAGTCTC ACTGGGAGGG AGTTCAAGAA
    GCTGAATACT GGCGTCGCAA AATAGCCATG TCGCCAGTGA TGCTATCATA TGGTCACTGT
    CAAGCTGTCC CAAAACAAGA GCAAGCAGTA TATGTCACAG ACCCCCAACC TGAACATGGA
    CTCAGTCCAT TTCCCAGCCC CCAAAGCAGC GGTTACCACA GCCCTCATCC ACCCTGTCCC
    TGCTCTCCAC AGCCGTTCAG AGACAGTCCA GGTTACGCCT CAATCAGTCA TTCCCAAAAC
    TCCTCCCCCA TTGCTCTCAC ACCATCTCCA AAACGAGCCA ATCACAATAG AGCCTCGGCT
    GAGGAGAACC AGCATGGCCC TAATGAGGAA TATACTAAGA TTTTATCTGA TGATAAGAGT
    CCTGCAACCT TTTCTCCACC TACCCAGAAC AGTGGACCAG TTAAAGCAGG AGAAATGGTG
    CAAATCAAAT CCCCAGAAGA TGTGAATCTC CCTGTGTCTC TGATCACCAA TATCTCCTCT
    GCCAGTGGAG CAGAAGATAA GACAACCATC ACAGATTGTA CATCTAACAG TCTTTTAATT
    AGTAGCCAAC CATCGTCAAA GAATATGGAG TCAGTGCAGG TACAAACGCT CAATCCCAGA
    CCTCTCAGTG TGCCAGAATC CGAGCAGGAT AAAGGAGAGC GTCCCGGCTC ATGCTACACC
    TCAACTACAA CCTCAACAAG TCCTCAACCT CCACTTTGCT CCTCAGAAAG AAGCTCTGCT
    TCAGAATTCT CATCATTTAA CTTAAACGGT GAAAGACAAA GCGTGACACC CTTGACTGAC
    TGCAATGAGG AAAGTAACAG TGACCCTGAT GGCTTCATCA CACCCTCATT TCCTATAGCA
    TCACACGGCT ATCCTCTTCT GACGGTGCCT CATGTCCCCT ACACAGGCTA CACTGAGATC
    ACCATACCGA CCAATTTAGC CCAACCTCCT CTTCCAGAAA AACAGCGCTC ATCGCCTCTA
    CACAACACGT CTGATTATAC TCCCACTTCC TTAATTAAAT CAGCTGGAAA GCCTTCGGTT
    TCCTCTGCAC AGGTTCATGT GTCTTTCTCC ACTACAACAA ATGAAGTGCC TCCCAAATCT
    ACATGCAGAA TACCCCTCTC TAGTGGTGTG GACGAGCCCG AGAGCCGTCT CAGCTCCAAG
    TTTGTCCAGG ACAGCTCCAA ATACTGGTAC AAACCTGGCA TCTCCCGGGA CCAGGCGATC
    GCAGTACTGA AGGATAAGGA GGCAGGCTGC TTCCTCATCA GGGATAGTAA TTCATTCCAG
    GGTGCGTATG GATTGGCACT GAAAGTGAGT ACAGCTCCAG CCCACACCAA TTCCCACACA
    GAGCCGGGCA GCGCACAGGA ACAGCTGGTC AGACACTTCC TGATCGAGAG CGGCGCTAAA
    GGAGTCAAGA TCAAGGGCTG CCAAAATGAA ACATATTTCG GCTCTCTGTC TGCTTTGGTG
    TATCAACACT CCATTACTCC TGTGTCTCTT CCTTGTGTCC TGCGTATTCC AGATCGAGAT
    CTTGTTGGTG AATTGCAAGA TTTGCAAAGT GGAACGAATA CCAGCACAGC AGCAGACCTG
    TTGAAACAGG GGGCAGCTTG TAACGTTCTC TACCTGAACT CAGTGGAAAC AGAGTCTCTG
    ACGGGCCCGC AGGCCGTCTC AAAAACAACC AAGTGCACTT TGACCCAGGA GCCTCGTCCT
    TCACCAACAG TCGTCCACTT CAAAGTGTCC ACTCAGGGCA TCACCCTCAC TGATAACCAG
    CGCAGACTGT TTTTCAGGCG ACATTACCCA ATTCACAGTG TGACGTTCAG CAGTGTGGAT
    CCACTGGATC AAAGGTGGGT TGATGCTGAT AACACTTCCT GCAAGATGTT TGGATTTGTT
    GCACGGCGCA GTGGTGCTTT GGGAAACGTG TGTCACCTGT TTGCAGAGCT CGACCCGGAG
    CAACCCGCCA CAGCCATCGT GAACTTCATC AATAAAGTCA TGCTGGGACC TCAACAGCTG
    CGAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009300850.1
    CDS100..4128
    Translation

    Target ORF information:

    RefSeq Version XM_009302575.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio tensin 2b (tns2b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009302575.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    ATGAATGTCC CTCAAAGTAA AGCCAGCATG GAGACCGTTA TGGAGAACCT GATGGGCAGT 
    CATTATGATT TCGACTTGAC CTACATCACT GAGCGAATCA TCTCTGTCCT GTATCAGTCT
    GACCTGGACG AACAGAGCTA CAGTGCTAAC CTAAAAGTAG TGGCGCCCAT GTTGAAATCT
    AAACATCAAG ACAAATTCTT GCTTATAAAT CTGTCAGAGA AACGTCATGA CATCTGCAGG
    CTAAACCCAA AGGTTGAGGA GTTTGGGTGG CCTGATTTGC ATGCCCCGCC CCTCGATAAA
    ATATGTGCCG TATGCAAGAC TATGGAGAAC TGGCTTAACT CTGACCCCAA CAATGTGGTT
    GTGCTGCACT GCAAGGGAAA CAAAGGAAAA ACAGGGGTGA TTGTGGCAGC TTACATGCAC
    TATAGCAAGA TATCAGCTGG GGCAGATCAG GCTCTCTCAA CAGTTGCTAT GAGGAAATTT
    TGTGAGGATA AAATCTCTTC TTTTATCCTG CCATCCCAGA ACAGGTATAT ATATTATTTT
    GCCGGTCTCC TCTCGGGCTC AATAAAGATG AACAGCTCAC CTCTGTTCCT GCATCAGATT
    CATGTTCCAG TTTCACCCAA CTACCAGTCA GGAGGAGGTT ATGCTCCCTT TCTGAAGATC
    TATCAGTCGC TGCAGCTGGT CTATTCTTCA GGCAAATATG ATATTCAGGG CCCTAACAGT
    AAGACGCTGT GTGTAAATAT TGAACCAGCT CTGCTGTTGA AGGGAGACAT CCTGGTGAAG
    TGTTATCACC GCCTCTCAGA TGTGTGTAAG AGAGAATGTG TGTTCAGAGT CCAGTTTCAC
    ACCTGTAGTG TTCATGGATC TCAGCTTGCG TTTGGCAAAG GGGAGCTGGA CCATGCCTGC
    ACAGATGATC GGTTTCCTTC AGATGCAACA GTGGAGTTGC TGTTTTCTAC TGGCCCTCAA
    AGGAGAGGTG GAGATGTGCC AGGGAATGAG CCAGGAGTAT CTGCGGATTA TGGCAGCACA
    GATCCTGTTG TCCGCTGGGA CTCATATGAG AACTTCAACC TTCACCACGA GGACAGTGTT
    GAGGATATCA GTCACACACG TGGACCTTTA GATGGCAGTC TGTATGCTCA AGTGAAGAAG
    CGTCGAGCAC AGGGCTCCAG TCCCAGCAGA TGCCCGACCT CCATCAAACC CTCCACATAC
    TCACCCACTC ATTCCTCTTC TACTTCTCCA ATCCATCTCT CCGTCCAGCC TCTCTCACTC
    AGCAAAGACA CAAGTCGCTC ATCTGCAGCC CCTGAATCCC TCACACCACT TCCCAGAGAA
    AAAAGAGAGA AAGATGAAAA CAGGGAAAGA CACAGAGAAA CTGCGATACT TGATGATGGA
    GAATGCTCTC CACTCAGACC TGATCGGCCT GTTCAGTCTT GCTATGGTCA CCCGTTTTCA
    CACACTCACT CATACTGTGG CCCAGATCTG ACTAGCCCAC ATGCAATTGC AGTTCAACCC
    AAACATCGCA CGCTTCCCTG CAACCGCACA ACCCCATTGT CAGTCCGCGA CCTGTGTGTA
    TCCCAGCCAG ATCTTTTATG GGAGAGAGGG CGCTGTTTGC ACCATCCCTG CCCAGAATCT
    GTCAGACACC TCTACTCGTA TCCTCCACAA GAAACACACA CTCATTCGCC AATCCCACAG
    ACTTCCCATT CCCACACACT TCCTCCACAA ACACGTGCGT ATTTCTCTGG GGAGGCGTGC
    CCTTTGTTTC ATTGCTCCGC CCTTCCTCAG GGCCACAACC ACATCCCTCC TTCTCTGTCC
    CCTAATCAGT CCCTACTCAC CAGCCCTTAC AGAGAGTTAC GATACAGCAC AACATCACCA
    ACTTCATGCT CTTGTCGGGA TTGTTTACGA TTACGAGAGG ATGTTGCCCT CCATTCTTTA
    CGAGGAAGGG AATTAGAAAC CCTTCCTTGG TCTAGAGAGG CGGAGTTTGG AATCCGAAGG
    GAGGCACCTG TGCATTGGAG GGATGGGAGG GCGGAGTCTC ACTGGGAGGG AGTTCAAGAA
    GCTGAATACT GGCGTCGCAA AATAGCCATG TCGCCAGTGA TGCTATCATA TGGTCACTGT
    CAAGCTGTCC CAAAACAAGA GCAAGCAGTA TATGTCACAG ACCCCCAACC TGAACATGGA
    CTCAGTCCAT TTCCCAGCCC CCAAAGCAGC GGTTACCACA GCCCTCATCC ACCCTGTCCC
    TGCTCTCCAC AGCCGTTCAG AGACAGTCCA GGTTACGCCT CAATCAGTCA TTCCCAAAAC
    TCCTCCCCCA TTGCTCTCAC ACCATCTCCA AAACGAGCCA ATCACAATAG AGCCTCGGCT
    GAGGAGAACC AGCATGGCCC TAATGAGGAA TATACTAAGA TTTTATCTGA TGATAAGAGT
    CCTGCAACCT TTTCTCCACC TACCCAGAAC AGTGGACCAG TTAAAGCAGG AGAAATGGTG
    CAAATCAAAT CCCCAGAAGA TGTGAATCTC CCTGTGTCTC TGATCACCAA TATCTCCTCT
    GCCAGTGGAG CAGAAGATAA GACAACCATC ACAGATTGTA CATCTAACAG TCTTTTAATT
    AGTAGCCAAC CATCGTCAAA GAATATGGAG TCAGTGCAGG TACAAACGCT CAATCCCAGA
    CCTCTCAGTG TGCCAGAATC CGAGCAGGAT AAAGGAGAGC GTCCCGGCTC ATGCTACACC
    TCAACTACAA CCTCAACAAG TCCTCAACCT CCACTTTGCT CCTCAGAAAG AAGCTCTGCT
    TCAGAATTCT CATCATTTAA CTTAAACGGT GAAAGACAAA GCGTGACACC CTTGACTGAC
    TGCAATGAGG AAAGTAACAG TGACCCTGAT GGCTTCATCA CACCCTCATT TCCTATAGCA
    TCACACGGCT ATCCTCTTCT GACGGTGCCT CATGTCCCCT ACACAGGCTA CACTGAGATC
    ACCATACCGA CCAATTTAGC CCAACCTCCT CTTCCAGAAA AACAGCGCTC ATCGCCTCTA
    CACAACACGT CTGATTATAC TCCCACTTCC TTAATTAAAT CAGCTGGAAA GCCTTCGGTT
    TCCTCTGCAC AGGTTCATGT GTCTTTCTCC ACTACAACAA ATGAAGTGCC TCCCAAATCT
    ACATGCAGAA TACCCCTCTC TAGTGGTGTG GACGAGCCCG AGAGCCGTCT CAGCTCCAAG
    TTTGTCCAGG ACAGCTCCAA ATACTGGTAC AAACCTGGCA TCTCCCGGGA CCAGGCGATC
    GCAGTACTGA AGGATAAGGA GGCAGGCTGC TTCCTCATCA GGGATAGTAA TTCATTCCAG
    GGTGCGTATG GATTGGCACT GAAAGTGAGT ACAGCTCCAG CCCACACCAA TTCCCACACA
    GAGCCGGGCA GCGCACAGGA ACAGCTGGTC AGACACTTCC TGATCGAGAG CGGCGCTAAA
    GGAGTCAAGA TCAAGGGCTG CCAAAATGAA ACATATTTCG GCTCTCTGTC TGCTTTGGTG
    TATCAACACT CCATTACTCC TGTGTCTCTT CCTTGTGTCC TGCGTATTCC AGATCGAGAT
    CTTGTTGGTG AATTGCAAGA TTTGCAAAGT GGAACGAATA CCAGCACAGC AGCAGACCTG
    TTGAAACAGG GGGCAGCTTG TAACGTTCTC TACCTGAACT CAGTGGAAAC AGAGTCTCTG
    ACGGGCCCGC AGGCCGTCTC AAAAACAACC AAGTGCACTT TGACCCAGGA GCCTCGTCCT
    TCACCAACAG TCGTCCACTT CAAAGTGTCC ACTCAGGGCA TCACCCTCAC TGATAACCAG
    CGCAGACTGT TTTTCAGGCG ACATTACCCA ATTCACAGTG TGACGTTCAG CAGTGTGGAT
    CCACTGGATC AAAGGTGGGT TGATGCTGAT AACACTTCCT GCAAGATGTT TGGATTTGTT
    GCACGGCGCA GTGGTGCTTT GGGAAACGTG TGTCACCTGT TTGCAGAGCT CGACCCGGAG
    CAACCCGCCA CAGCCATCGT GAACTTCATC AATAAAGTCA TGCTGGGACC TCAACAGCTG
    CGAAAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.