TNS2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_005206246.3	XP_005206303.3	tensin-2 isoform X5
XM_005198237.3	XP_005198294.1	tensin-2 isoform X4
XM_005206244.3	XP_005206301.3	tensin-2 isoform X1
XM_005206245.4	XP_005206302.2	tensin-2 isoform X5
XM_024992422.1	XP_024848190.1	tensin-2 isoform X9
XM_005206245.3	XP_005206302.1	tensin-2 isoform X7
XM_005198238.3	XP_005198295.3	tensin-2 isoform X2
XM_001790201.5	XP_001790253.1	tensin-2 isoform X6
XM_024992419.1	XP_024848187.1	tensin-2 isoform X3
XM_005206246.4	XP_005206303.3	tensin-2 isoform X4
XM_024992421.1	XP_024848189.1	tensin-2 isoform X8
XM_015463275.1	XP_015318761.1	tensin-2 isoform X1
XM_015470963.1	XP_015326449.1	tensin-2 isoform X3
XM_015470962.1	XP_015326448.1	tensin-2 isoform X1
XM_024992420.1	XP_024848188.1	tensin-2 isoform X7
XM_005206243.4	XP_005206300.1	tensin-2 isoform X2
XM_024992423.1	XP_024848191.1	tensin-2 isoform X10
XM_015463276.1	XP_015318762.1	tensin-2 isoform X3
XM_005198240.3	XP_005198297.3	tensin-2 isoform X5
XM_002687243.4	XP_002687289.1	tensin-2 isoform X6
XM_002687243.5	XP_002687289.1	tensin-2 isoform X6
XM_005206243.3	XP_005206300.1	tensin-2 isoform X4
XM_024992424.1	XP_024848192.1	tensin-2 isoform X11
XM_024992425.1	XP_024848193.1	tensin-2 isoform X12

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa04205	XM_005198237.3 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa04205	XM_005206243.4 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa04206	XM_002687243.5 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa04206	XM_001790201.5 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa04208	XM_005206245.3 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234736	XM_024992419.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234737	XM_005206245.4 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234738	XM_024992420.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234739	XM_024992421.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234740	XM_024992422.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234741	XM_024992423.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234742	XM_024992424.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa234743	XM_024992425.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa89855	XM_015463275.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89855	XM_015470962.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89856	XM_005206244.3 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89856	XM_005198238.3 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89857	XM_015470963.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89857	XM_015463276.1 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa89858	XM_005206246.4 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa89858	XM_005198240.3 Latest version!	Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
View Old Accession Versions >>
OBa89858	XM_005206246.3	Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa04206	XM_002687243.4	Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
OBa04205	XM_005206243.3	Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
Hide Old Accession Versions >>

TNS2 ( XM_005198237.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198294.1 Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa04205
Clone ID Related Accession (Same CDS sequence)	XM_005198237.3 , XM_005206243.4 , XM_005206243.3
Accession Version	XM_005198237.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644788.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198237.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198294.1
CDS	351..4595
Translation	MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005198237.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005198237.3

ORF Insert Sequence:

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005206243.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206300.1 Bos taurus tensin 2 (TNS2), transcript variant X2, mRNA.

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CloneID	OBa04205
Clone ID Related Accession (Same CDS sequence)	XM_005198237.3 , XM_005206243.4 , XM_005206243.3
Accession Version	XM_005206243.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206243.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206300.1
CDS	338..4582
Translation	MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005206243.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206243.4

ORF Insert Sequence:

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005206243.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206300.1 Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa04205
Clone ID Related Accession (Same CDS sequence)	XM_005198237.3 , XM_005206243.4 , XM_005206243.3
Accession Version	XM_005206243.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103919.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206243.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206300.1
CDS	351..4595
Translation	MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005206243.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206243.3

ORF Insert Sequence:

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_001790201.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_001790253.1 Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.

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CloneID	OBa04206
Clone ID Related Accession (Same CDS sequence)	XM_001790201.5 , XM_002687243.4 , XM_002687243.5
Accession Version	XM_001790201.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644788.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_001790201.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001790253.1
CDS	350..4579
Translation	MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_001790201.5
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001790201.5

ORF Insert Sequence:

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_002687243.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002687289.1 Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa04206
Clone ID Related Accession (Same CDS sequence)	XM_001790201.5 , XM_002687243.4 , XM_002687243.5
Accession Version	XM_002687243.4	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103919.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_002687243.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002687289.1
CDS	350..4579
Translation	MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_002687243.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002687243.4

ORF Insert Sequence:

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_002687243.5 ) cDNA ORF clone, Bos taurus(cattle) -> XP_002687289.1 Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa04206
Clone ID Related Accession (Same CDS sequence)	XM_001790201.5 , XM_002687243.4 , XM_002687243.5
Accession Version	XM_002687243.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_002687243.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002687289.1
CDS	338..4567
Translation	MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_002687243.5
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002687243.5

ORF Insert Sequence:

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005206245.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206302.1 Bos taurus tensin 2 (TNS2), transcript variant X7, mRNA.

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CloneID	OBa04208
Clone ID Related Accession (Same CDS sequence)	XM_005206245.3
Accession Version	XM_005206245.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4161bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103919.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206245.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCCTA GGAAAGCTGA GCCGCATAGC TTCCGAGAGA AGGTCTTCCG GAAGAAACCA 
CCCGTGTGTG CCGTGTGTAA GGCAGCCATT GATGGGACGG GCGTCTCATG CAGAGTCTGC 
AAGGTGGCAA CACACAGAAA ATGTGAAGCA AAGGTGACTT CGTCCTGTCA GGCTTTGCCT 
CCCGTGGAGC TGCGGAGAAA CACCGCCCCT GTGAGGCGCA TAGAGCACCT GGGATCCACC 
AAGTCTCTGA ACCACTCAAA GCAGCGCAGC ACTCTGCCCA GGAGCTTCAG CCTGGACCCG 
CTCATGGAGC GCCGCTGGGA CTTGGACCTC ACCTACGTGA CGGAGCGGAT CCTTGCCGCC 
TCTTTCCCCG CGCGGCCTGA CGAGCAGCGA CACCGGGGCC ACCTGCGCGA GCTGGCTCAC 
GTGCTGCAAT CCAAGCACCG TGACAAGTAC CTGCTCTTCA ACCTTTCAGA GAAAAGACAT 
GACCTGACCC GCCTAAACCC GAAGGTCCAG GACTTTGGCT GGCCTGAGCT GCACGCACCC 
CCCCTAGACA AGCTGTGCTC CATTTGCAAA GCCATGGAGA CCTGGCTCAG TGCTGACCCG 
CAGCACGTGG TCGTATTGTA CTGCAAGGGG AGCAAGGGCA AGCTCGGGGT CATCGTCTCT 
GCCTACATGC ACTACAGCAA GATCTCTGCA GGGGCAGACC AGGCACTGGC GACCCTTACC 
ATGCGGAAGT TCTGTGAAGA CAAGGTTGCC GCAGAATTGC AGCCCTCTCA GCGCCGGTAT 
ATCACCTACT TCAGCGGTCT GCTGTCCGGG TCCATCCGCA TGAACAGCAG CCCTCTCTTC 
CTGCACCACG TGCTGGTGCC CGTGCTGCCA GCCTTTGAAC CTGGTGCAGG ATTCCAGCCC 
TTCCTCAAGA TCTACCAGTC CATGCAGCTT GTTTACACAT CAGGAATCTA TCACATTGCA 
GGCCCTGGTC CCCAGCAGCT TTGTATCAGC CTGGAGCCAG CCCTCCTCCT CAAAGGCGAT 
GTCATGGTAA CATGCTATCA CAAGGGTAGC CGGGGAACTG ACCGGACCCT CGTATTCCGA 
GTCCAGTTCC ACACATGCAC CATCCATGGA CCACGGCTCA CCTTCTCCAA GGACCAGTTG 
GATGAGGCCT GGACCGACGA GAGGTTCCCC TTCCAAGCCT CGGTGGAGTT CGTCTTCTCC 
TCCAGCCCAG AGAAGATCAA AGGTAACACC CCACAGAATG AGCCCTCCGT CTCTGTCGAC 
TACAACACTG CAGAGCCCGC AGTGCGCTGG GACTCCTACG AGAACTTCAA CTTGCACCAT 
GAGGACAGTG CGGATGACTC TGTCACCCAC ACCCGGGGGC CCCTGGATGG CAGTCCTTAT 
GCCCAGGTGC AGCGAGCCCC CCGCCAGACC CCGCCGGCCC CGTCTCCGGA GCCACCCCCA 
CCCCCGCTGC TTTCTGTCAG CAGCGACTCT GGCCACTCCT CCACACTGAC CACAGAGCCA 
GCTGCCGAGT CCCCTGGCCG GCCACCCCCG ACGGCTGCCG AGCGGCAGGA GCTCGAGCGC 
CTACTGGGAG GCTGTGGAGT GGCTAGTGGG GGCCGGGGAG CTGGGCGCGA GACGGCCATC 
CTTGATGACG AAGACCAGCC TGCTGCAGGC GGAGGCCCCC ACCTCGGAAT ATATTCGGGA 
CACAGGCCTG GCCTCAGCCG CCACTGCTCC TGCCGCCAGG GCTACCGGGA ACCCTGCGGG 
GTCCCCAATG GAGGTTACTA CCGGCCAGAG GGGACCCTGG AGAGGCGGCG GCTGGCTTTC 
GGAGCCTATG AGGGGCCCCC CCAGGGTTAT GCTGAGCCCT CCGTGGAGAA GAGACGCCTC 
TGCCGCTCGC TGTCCGAGGG GCCGTACCCC TACCCGTCTG AGCTGGGGAA ACCCGCCAAT 
GGAGACTTTG GCTACCGCCC CCCAGGCTAC CGGGAGGTGG TGATCCTGGA AGACCCGGGG 
CTGCCTCCGC TGTGCTCGTG CCCCGCCTGT GAGGAGAAGC TAGCACTGCC CACGGCAGCC 
CTCTATGGGC TGCGGCTGGA GAGGGAGGCT GGAGAGGGGT GGGCAAGTGA GGCTGGCAAG 
CCCCTCCTGC ACCCAGTGCG ACCCGGGCAC CCGCTGCCCC TGCTGGTGCC TGCCTGTGGG 
CACCACCACA CCCCGCTGCC TGACTACAGC TGCTTGAAGC CACCCAAGGC AGGCGAGGAA 
GGGCATGAGG GCTGCTCCTA CGCCTTGTGC CCCGAGGGAA GGTATGGGCA CCCAGGGTAC 
CCTGCCCTGG TGACGTACGG CTATGGAGGA GCAGTTCCCA GTTACTGCCC AGCATATGGC 
CGGGTGCCGC ACAGCTGTGG GTCTCCAGGT GAGGGCAGAG GGTATCCCAG CCCTCGTGCC 
CACTCCCCCC GGGCTGGCTC CATTTCCCCG GGCAGCCCGC CCTACCCCCA GTCCAGGAAG 
CTGAGCTACG AGATCCCTGC AGAGGAGGGA GGGGACCGGT ATCTGCTGCC TGGGCACCTG 
GCCCCAGCAG GACCCTTGGC ATCTGCAGAG TCGCCAGAGC CTGTGTCCTG GAGGGAGGGT 
CCCAGCGGGC ACAGCACCCT GCCCCGGTCC CCGCGAGATG CCCAGTGCAG CGCTGCTTCC 
GAGCTGTCTG GTCCTTCCAC GCCGCTGCAC ACCAGCAGCC CCGTCCAGGG CAAGGAGAGC 
GCCCGACGGC AGGACACCCG CTCCCCCACC TTGGCGCCCA CTCAGAGACT GAGTCCTGCG 
GAGGCCTTGC CACCTGTTTC CCAGGGAGGC GCTGATAAGG CTCCAGAGCT GCCTGCAAGA 
AGTGGGCCTG AGCCTCCAGC CCCTGGCGCC TTCTCCCTAG CCTCCCCACC CAGCTCTCCC 
AACGACTGGC CTCAGGAGAG GAGCCCGGGG GGCCGCTCAG ACAGCGCCAG TCCAAGGGGG 
CCTGTACCCA ACACCCTGCC CGGCCTCCGT CATGCCCCCT GGCAGGGCCT GCGAGACCCC 
CCGGACAGCC CAGATGGGTC CCCCCTCACC CCTGTGCCTA CTCAGATGCC CTGGCTTGTG 
GCCAGCCCAG AACCCCCTCA GAGCTCGCCC ACACCTGCCT TTCCTCTGGC TGCATCTTAC 
GACATCAACG GCGCCCCCCA GCCCCCTCTT CCTGAGAAAC GCCACCTGCT GGGGCCTGGG 
CAGCAGCCGG GACCCTGGGG CCCAGAGCAG GCGTCCCCAC CAGCCAGAGG CGCTAGTCAC 
CATGTCACTT TTGCACCTCT GCTCCCGGAT AACACCCCCC AGCCCCCAGA GCCCCCGATG 
CAAGAGAGCC AGAGCAATGT CAAGTTTGTC CAGGACACAT CCAAGTTCTG GTACAAGCCG 
CACCTGTCCC GTGACCAAGC CATCACCCTG CTGAAGGACA AAGACCCTGG GGCCTTCCTG 
ATCAGGGACA GTCATTCATT CCAAGGAGCC TATGGGCTGG CTCTCAAGGT GGCCACGCCC 
CCTCCCAGCG CCCAGTCCTG GAAAGGGGAC CCCTCAGAAC AGCTGGTCCG TCATTTTCTC 
ATTGAGACTG GGCCGAAAGG GGTGAAGATC AAGGGCTGTC CCAGCGAGCC CTACTTTGGC 
AGCCTGTCAG CCCTGGTCTC CCAGCACTCC ATCTCCCCAC TGTCCCTGCC CTGCTGCCTG 
CGCATTCCCA GCAAAGATCC TCTGGAGGAG GTCCCAGAGG CCCCAGTGCC CAGCAACATG 
AGTACAGCAG CAGACCTCCT GCGTCAAGGC GCCGCCTGCA GCGTGCTCTA CCTGACCTCA 
GTGGAGACGG AGTCACTGAC GGGCCCCCAA GCAGTGGCAC GGGCCAGCTC CGCAGCTCTG 
AGCTGCAGCC CCCGCCCCAC ACCAGCCGTT GTCCACTTCA AGGTCTCAGC CCAGGGCATC 
ACACTCACAG ACAACCAAAG GAAGCTCTTC TTTCGCCGCC ATTATCCAGT CAACAGCATC 
ACCTTTTCCA GCACTGACCC TCAGGACCGG AGATGGACCA ACTCCGACGG GACCACCTCC 
AAGATCTTTG GTTTTGTGGC CAAGAAGCCG GGAAGCCCTT GGGAGAACGT GTGTCACCTC 
TTTGCAGAGC TTGACCCAGA TCAGCCCGCA GGCGCCATTG TCACCTTCAT CACCAAAGTT 
TTACTGGGCC AGAGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206302.1
CDS	11..4171
Translation	MKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005206245.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206245.3

ORF Insert Sequence:

ATGAAGCCTA GGAAAGCTGA GCCGCATAGC TTCCGAGAGA AGGTCTTCCG GAAGAAACCA 
CCCGTGTGTG CCGTGTGTAA GGCAGCCATT GATGGGACGG GCGTCTCATG CAGAGTCTGC 
AAGGTGGCAA CACACAGAAA ATGTGAAGCA AAGGTGACTT CGTCCTGTCA GGCTTTGCCT 
CCCGTGGAGC TGCGGAGAAA CACCGCCCCT GTGAGGCGCA TAGAGCACCT GGGATCCACC 
AAGTCTCTGA ACCACTCAAA GCAGCGCAGC ACTCTGCCCA GGAGCTTCAG CCTGGACCCG 
CTCATGGAGC GCCGCTGGGA CTTGGACCTC ACCTACGTGA CGGAGCGGAT CCTTGCCGCC 
TCTTTCCCCG CGCGGCCTGA CGAGCAGCGA CACCGGGGCC ACCTGCGCGA GCTGGCTCAC 
GTGCTGCAAT CCAAGCACCG TGACAAGTAC CTGCTCTTCA ACCTTTCAGA GAAAAGACAT 
GACCTGACCC GCCTAAACCC GAAGGTCCAG GACTTTGGCT GGCCTGAGCT GCACGCACCC 
CCCCTAGACA AGCTGTGCTC CATTTGCAAA GCCATGGAGA CCTGGCTCAG TGCTGACCCG 
CAGCACGTGG TCGTATTGTA CTGCAAGGGG AGCAAGGGCA AGCTCGGGGT CATCGTCTCT 
GCCTACATGC ACTACAGCAA GATCTCTGCA GGGGCAGACC AGGCACTGGC GACCCTTACC 
ATGCGGAAGT TCTGTGAAGA CAAGGTTGCC GCAGAATTGC AGCCCTCTCA GCGCCGGTAT 
ATCACCTACT TCAGCGGTCT GCTGTCCGGG TCCATCCGCA TGAACAGCAG CCCTCTCTTC 
CTGCACCACG TGCTGGTGCC CGTGCTGCCA GCCTTTGAAC CTGGTGCAGG ATTCCAGCCC 
TTCCTCAAGA TCTACCAGTC CATGCAGCTT GTTTACACAT CAGGAATCTA TCACATTGCA 
GGCCCTGGTC CCCAGCAGCT TTGTATCAGC CTGGAGCCAG CCCTCCTCCT CAAAGGCGAT 
GTCATGGTAA CATGCTATCA CAAGGGTAGC CGGGGAACTG ACCGGACCCT CGTATTCCGA 
GTCCAGTTCC ACACATGCAC CATCCATGGA CCACGGCTCA CCTTCTCCAA GGACCAGTTG 
GATGAGGCCT GGACCGACGA GAGGTTCCCC TTCCAAGCCT CGGTGGAGTT CGTCTTCTCC 
TCCAGCCCAG AGAAGATCAA AGGTAACACC CCACAGAATG AGCCCTCCGT CTCTGTCGAC 
TACAACACTG CAGAGCCCGC AGTGCGCTGG GACTCCTACG AGAACTTCAA CTTGCACCAT 
GAGGACAGTG CGGATGACTC TGTCACCCAC ACCCGGGGGC CCCTGGATGG CAGTCCTTAT 
GCCCAGGTGC AGCGAGCCCC CCGCCAGACC CCGCCGGCCC CGTCTCCGGA GCCACCCCCA 
CCCCCGCTGC TTTCTGTCAG CAGCGACTCT GGCCACTCCT CCACACTGAC CACAGAGCCA 
GCTGCCGAGT CCCCTGGCCG GCCACCCCCG ACGGCTGCCG AGCGGCAGGA GCTCGAGCGC 
CTACTGGGAG GCTGTGGAGT GGCTAGTGGG GGCCGGGGAG CTGGGCGCGA GACGGCCATC 
CTTGATGACG AAGACCAGCC TGCTGCAGGC GGAGGCCCCC ACCTCGGAAT ATATTCGGGA 
CACAGGCCTG GCCTCAGCCG CCACTGCTCC TGCCGCCAGG GCTACCGGGA ACCCTGCGGG 
GTCCCCAATG GAGGTTACTA CCGGCCAGAG GGGACCCTGG AGAGGCGGCG GCTGGCTTTC 
GGAGCCTATG AGGGGCCCCC CCAGGGTTAT GCTGAGCCCT CCGTGGAGAA GAGACGCCTC 
TGCCGCTCGC TGTCCGAGGG GCCGTACCCC TACCCGTCTG AGCTGGGGAA ACCCGCCAAT 
GGAGACTTTG GCTACCGCCC CCCAGGCTAC CGGGAGGTGG TGATCCTGGA AGACCCGGGG 
CTGCCTCCGC TGTGCTCGTG CCCCGCCTGT GAGGAGAAGC TAGCACTGCC CACGGCAGCC 
CTCTATGGGC TGCGGCTGGA GAGGGAGGCT GGAGAGGGGT GGGCAAGTGA GGCTGGCAAG 
CCCCTCCTGC ACCCAGTGCG ACCCGGGCAC CCGCTGCCCC TGCTGGTGCC TGCCTGTGGG 
CACCACCACA CCCCGCTGCC TGACTACAGC TGCTTGAAGC CACCCAAGGC AGGCGAGGAA 
GGGCATGAGG GCTGCTCCTA CGCCTTGTGC CCCGAGGGAA GGTATGGGCA CCCAGGGTAC 
CCTGCCCTGG TGACGTACGG CTATGGAGGA GCAGTTCCCA GTTACTGCCC AGCATATGGC 
CGGGTGCCGC ACAGCTGTGG GTCTCCAGGT GAGGGCAGAG GGTATCCCAG CCCTCGTGCC 
CACTCCCCCC GGGCTGGCTC CATTTCCCCG GGCAGCCCGC CCTACCCCCA GTCCAGGAAG 
CTGAGCTACG AGATCCCTGC AGAGGAGGGA GGGGACCGGT ATCTGCTGCC TGGGCACCTG 
GCCCCAGCAG GACCCTTGGC ATCTGCAGAG TCGCCAGAGC CTGTGTCCTG GAGGGAGGGT 
CCCAGCGGGC ACAGCACCCT GCCCCGGTCC CCGCGAGATG CCCAGTGCAG CGCTGCTTCC 
GAGCTGTCTG GTCCTTCCAC GCCGCTGCAC ACCAGCAGCC CCGTCCAGGG CAAGGAGAGC 
GCCCGACGGC AGGACACCCG CTCCCCCACC TTGGCGCCCA CTCAGAGACT GAGTCCTGCG 
GAGGCCTTGC CACCTGTTTC CCAGGGAGGC GCTGATAAGG CTCCAGAGCT GCCTGCAAGA 
AGTGGGCCTG AGCCTCCAGC CCCTGGCGCC TTCTCCCTAG CCTCCCCACC CAGCTCTCCC 
AACGACTGGC CTCAGGAGAG GAGCCCGGGG GGCCGCTCAG ACAGCGCCAG TCCAAGGGGG 
CCTGTACCCA ACACCCTGCC CGGCCTCCGT CATGCCCCCT GGCAGGGCCT GCGAGACCCC 
CCGGACAGCC CAGATGGGTC CCCCCTCACC CCTGTGCCTA CTCAGATGCC CTGGCTTGTG 
GCCAGCCCAG AACCCCCTCA GAGCTCGCCC ACACCTGCCT TTCCTCTGGC TGCATCTTAC 
GACATCAACG GCGCCCCCCA GCCCCCTCTT CCTGAGAAAC GCCACCTGCT GGGGCCTGGG 
CAGCAGCCGG GACCCTGGGG CCCAGAGCAG GCGTCCCCAC CAGCCAGAGG CGCTAGTCAC 
CATGTCACTT TTGCACCTCT GCTCCCGGAT AACACCCCCC AGCCCCCAGA GCCCCCGATG 
CAAGAGAGCC AGAGCAATGT CAAGTTTGTC CAGGACACAT CCAAGTTCTG GTACAAGCCG 
CACCTGTCCC GTGACCAAGC CATCACCCTG CTGAAGGACA AAGACCCTGG GGCCTTCCTG 
ATCAGGGACA GTCATTCATT CCAAGGAGCC TATGGGCTGG CTCTCAAGGT GGCCACGCCC 
CCTCCCAGCG CCCAGTCCTG GAAAGGGGAC CCCTCAGAAC AGCTGGTCCG TCATTTTCTC 
ATTGAGACTG GGCCGAAAGG GGTGAAGATC AAGGGCTGTC CCAGCGAGCC CTACTTTGGC 
AGCCTGTCAG CCCTGGTCTC CCAGCACTCC ATCTCCCCAC TGTCCCTGCC CTGCTGCCTG 
CGCATTCCCA GCAAAGATCC TCTGGAGGAG GTCCCAGAGG CCCCAGTGCC CAGCAACATG 
AGTACAGCAG CAGACCTCCT GCGTCAAGGC GCCGCCTGCA GCGTGCTCTA CCTGACCTCA 
GTGGAGACGG AGTCACTGAC GGGCCCCCAA GCAGTGGCAC GGGCCAGCTC CGCAGCTCTG 
AGCTGCAGCC CCCGCCCCAC ACCAGCCGTT GTCCACTTCA AGGTCTCAGC CCAGGGCATC 
ACACTCACAG ACAACCAAAG GAAGCTCTTC TTTCGCCGCC ATTATCCAGT CAACAGCATC 
ACCTTTTCCA GCACTGACCC TCAGGACCGG AGATGGACCA ACTCCGACGG GACCACCTCC 
AAGATCTTTG GTTTTGTGGC CAAGAAGCCG GGAAGCCCTT GGGAGAACGT GTGTCACCTC 
TTTGCAGAGC TTGACCCAGA TCAGCCCGCA GGCGCCATTG TCACCTTCAT CACCAAAGTT 
TTACTGGGCC AGAGGAAATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_024992419.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848187.1 Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.

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CloneID	OBa234736
Clone ID Related Accession (Same CDS sequence)	XM_024992419.1
Accession Version	XM_024992419.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4245bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAC CCCAGACCAC GGAAGAGGGG GTTCAGCCTT CCGTGCCAAG ATGGGTTGCC 
CTCTGCGTAG TTAAGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848187.1
CDS	66..4310
Translation	MGSPQTTEEGVQPSVPRWVALCVVKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_024992419.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992419.1

ORF Insert Sequence:

ATGGGATCAC CCCAGACCAC GGAAGAGGGG GTTCAGCCTT CCGTGCCAAG ATGGGTTGCC 
CTCTGCGTAG TTAAGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGCTC CTCTCCCTGC AGAGTCGCCA 
GAGCCTGTGT CCTGGAGGGA GGGTCCCAGC GGGCACAGCA CCCTGCCCCG GTCCCCGCGA 
GATGCCCAGT GCAGCGCTGC TTCCGAGCTG TCTGGTCCTT CCACGCCGCT GCACACCAGC 
AGCCCCGTCC AGGGCAAGGA GAGCGCCCGA CGGCAGGACA CCCGCTCCCC CACCTTGGCG 
CCCACTCAGA GACTGAGTCC TGCGGAGGCC TTGCCACCTG TTTCCCAGGG AGGCGCTGAT 
AAGGCTCCAG AGCTGCCTGC AAGAAGTGGG CCTGAGCCTC CAGCCCCTGG CGCCTTCTCC 
CTAGCCTCCC CACCCAGCTC TCCCAACGAC TGGCCTCAGG AGAGGAGCCC GGGGGGCCGC 
TCAGACAGCG CCAGTCCAAG GGGGCCTGTA CCCAACACCC TGCCCGGCCT CCGTCATGCC 
CCCTGGCAGG GCCTGCGAGA CCCCCCGGAC AGCCCAGATG GGTCCCCCCT CACCCCTGTG 
CCTACTCAGA TGCCCTGGCT TGTGGCCAGC CCAGAACCCC CTCAGAGCTC GCCCACACCT 
GCCTTTCCTC TGGCTGCATC TTACGACATC AACGGCGCCC CCCAGCCCCC TCTTCCTGAG 
AAACGCCACC TGCTGGGGCC TGGGCAGCAG CCGGGACCCT GGGGCCCAGA GCAGGCGTCC 
CCACCAGCCA GAGGCGCTAG TCACCATGTC ACTTTTGCAC CTCTGCTCCC GGATAACACC 
CCCCAGCCCC CAGAGCCCCC GATGCAAGAG AGCCAGAGCA ATGTCAAGTT TGTCCAGGAC 
ACATCCAAGT TCTGGTACAA GCCGCACCTG TCCCGTGACC AAGCCATCAC CCTGCTGAAG 
GACAAAGACC CTGGGGCCTT CCTGATCAGG GACAGTCATT CATTCCAAGG AGCCTATGGG 
CTGGCTCTCA AGGTGGCCAC GCCCCCTCCC AGCGCCCAGT CCTGGAAAGG GGACCCCTCA 
GAACAGCTGG TCCGTCATTT TCTCATTGAG ACTGGGCCGA AAGGGGTGAA GATCAAGGGC 
TGTCCCAGCG AGCCCTACTT TGGCAGCCTG TCAGCCCTGG TCTCCCAGCA CTCCATCTCC 
CCACTGTCCC TGCCCTGCTG CCTGCGCATT CCCAGCAAAG ATCCTCTGGA GGAGGTCCCA 
GAGGCCCCAG TGCCCAGCAA CATGAGTACA GCAGCAGACC TCCTGCGTCA AGGCGCCGCC 
TGCAGCGTGC TCTACCTGAC CTCAGTGGAG ACGGAGTCAC TGACGGGCCC CCAAGCAGTG 
GCACGGGCCA GCTCCGCAGC TCTGAGCTGC AGCCCCCGCC CCACACCAGC CGTTGTCCAC 
TTCAAGGTCT CAGCCCAGGG CATCACACTC ACAGACAACC AAAGGAAGCT CTTCTTTCGC 
CGCCATTATC CAGTCAACAG CATCACCTTT TCCAGCACTG ACCCTCAGGA CCGGAGATGG 
ACCAACTCCG ACGGGACCAC CTCCAAGATC TTTGGTTTTG TGGCCAAGAA GCCGGGAAGC 
CCTTGGGAGA ACGTGTGTCA CCTCTTTGCA GAGCTTGACC CAGATCAGCC CGCAGGCGCC 
ATTGTCACCT TCATCACCAA AGTTTTACTG GGCCAGAGGA AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005206245.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206302.2 Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa234737
Clone ID Related Accession (Same CDS sequence)	XM_005206245.4
Accession Version	XM_005206245.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206245.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGA GTCGCCAGAG 
CCTGTGTCCT GGAGGGAGGG TCCCAGCGGG CACAGCACCC TGCCCCGGTC CCCGCGAGAT 
GCCCAGTGCA GCGCTGCTTC CGAGCTGTCT GGTCCTTCCA CGCCGCTGCA CACCAGCAGC 
CCCGTCCAGG GCAAGGAGAG CGCCCGACGG CAGGACACCC GCTCCCCCAC CTTGGCGCCC 
ACTCAGAGAC TGAGTCCTGC GGAGGCCTTG CCACCTGTTT CCCAGGGAGG CGCTGATAAG 
GCTCCAGAGC TGCCTGCAAG AAGTGGGCCT GAGCCTCCAG CCCCTGGCGC CTTCTCCCTA 
GCCTCCCCAC CCAGCTCTCC CAACGACTGG CCTCAGGAGA GGAGCCCGGG GGGCCGCTCA 
GACAGCGCCA GTCCAAGGGG GCCTGTACCC AACACCCTGC CCGGCCTCCG TCATGCCCCC 
TGGCAGGGCC TGCGAGACCC CCCGGACAGC CCAGATGGGT CCCCCCTCAC CCCTGTGCCT 
ACTCAGATGC CCTGGCTTGT GGCCAGCCCA GAACCCCCTC AGAGCTCGCC CACACCTGCC 
TTTCCTCTGG CTGCATCTTA CGACATCAAC GGCGCCCCCC AGCCCCCTCT TCCTGAGAAA 
CGCCACCTGC TGGGGCCTGG GCAGCAGCCG GGACCCTGGG GCCCAGAGCA GGCGTCCCCA 
CCAGCCAGAG GCGCTAGTCA CCATGTCACT TTTGCACCTC TGCTCCCGGA TAACACCCCC 
CAGCCCCCAG AGCCCCCGAT GCAAGAGAGC CAGAGCAATG TCAAGTTTGT CCAGGACACA 
TCCAAGTTCT GGTACAAGCC GCACCTGTCC CGTGACCAAG CCATCACCCT GCTGAAGGAC 
AAAGACCCTG GGGCCTTCCT GATCAGGGAC AGTCATTCAT TCCAAGGAGC CTATGGGCTG 
GCTCTCAAGG TGGCCACGCC CCCTCCCAGC GCCCAGTCCT GGAAAGGGGA CCCCTCAGAA 
CAGCTGGTCC GTCATTTTCT CATTGAGACT GGGCCGAAAG GGGTGAAGAT CAAGGGCTGT 
CCCAGCGAGC CCTACTTTGG CAGCCTGTCA GCCCTGGTCT CCCAGCACTC CATCTCCCCA 
CTGTCCCTGC CCTGCTGCCT GCGCATTCCC AGCAAAGATC CTCTGGAGGA GGTCCCAGAG 
GCCCCAGTGC CCAGCAACAT GAGTACAGCA GCAGACCTCC TGCGTCAAGG CGCCGCCTGC 
AGCGTGCTCT ACCTGACCTC AGTGGAGACG GAGTCACTGA CGGGCCCCCA AGCAGTGGCA 
CGGGCCAGCT CCGCAGCTCT GAGCTGCAGC CCCCGCCCCA CACCAGCCGT TGTCCACTTC 
AAGGTCTCAG CCCAGGGCAT CACACTCACA GACAACCAAA GGAAGCTCTT CTTTCGCCGC 
CATTATCCAG TCAACAGCAT CACCTTTTCC AGCACTGACC CTCAGGACCG GAGATGGACC 
AACTCCGACG GGACCACCTC CAAGATCTTT GGTTTTGTGG CCAAGAAGCC GGGAAGCCCT 
TGGGAGAACG TGTGTCACCT CTTTGCAGAG CTTGACCCAG ATCAGCCCGC AGGCGCCATT 
GTCACCTTCA TCACCAAAGT TTTACTGGGC CAGAGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206302.2
CDS	3..4244
Translation	MCWEGGLLSSPPAQGQLLRKESGAGGAMKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005206245.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206245.4

ORF Insert Sequence:

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGA GTCGCCAGAG 
CCTGTGTCCT GGAGGGAGGG TCCCAGCGGG CACAGCACCC TGCCCCGGTC CCCGCGAGAT 
GCCCAGTGCA GCGCTGCTTC CGAGCTGTCT GGTCCTTCCA CGCCGCTGCA CACCAGCAGC 
CCCGTCCAGG GCAAGGAGAG CGCCCGACGG CAGGACACCC GCTCCCCCAC CTTGGCGCCC 
ACTCAGAGAC TGAGTCCTGC GGAGGCCTTG CCACCTGTTT CCCAGGGAGG CGCTGATAAG 
GCTCCAGAGC TGCCTGCAAG AAGTGGGCCT GAGCCTCCAG CCCCTGGCGC CTTCTCCCTA 
GCCTCCCCAC CCAGCTCTCC CAACGACTGG CCTCAGGAGA GGAGCCCGGG GGGCCGCTCA 
GACAGCGCCA GTCCAAGGGG GCCTGTACCC AACACCCTGC CCGGCCTCCG TCATGCCCCC 
TGGCAGGGCC TGCGAGACCC CCCGGACAGC CCAGATGGGT CCCCCCTCAC CCCTGTGCCT 
ACTCAGATGC CCTGGCTTGT GGCCAGCCCA GAACCCCCTC AGAGCTCGCC CACACCTGCC 
TTTCCTCTGG CTGCATCTTA CGACATCAAC GGCGCCCCCC AGCCCCCTCT TCCTGAGAAA 
CGCCACCTGC TGGGGCCTGG GCAGCAGCCG GGACCCTGGG GCCCAGAGCA GGCGTCCCCA 
CCAGCCAGAG GCGCTAGTCA CCATGTCACT TTTGCACCTC TGCTCCCGGA TAACACCCCC 
CAGCCCCCAG AGCCCCCGAT GCAAGAGAGC CAGAGCAATG TCAAGTTTGT CCAGGACACA 
TCCAAGTTCT GGTACAAGCC GCACCTGTCC CGTGACCAAG CCATCACCCT GCTGAAGGAC 
AAAGACCCTG GGGCCTTCCT GATCAGGGAC AGTCATTCAT TCCAAGGAGC CTATGGGCTG 
GCTCTCAAGG TGGCCACGCC CCCTCCCAGC GCCCAGTCCT GGAAAGGGGA CCCCTCAGAA 
CAGCTGGTCC GTCATTTTCT CATTGAGACT GGGCCGAAAG GGGTGAAGAT CAAGGGCTGT 
CCCAGCGAGC CCTACTTTGG CAGCCTGTCA GCCCTGGTCT CCCAGCACTC CATCTCCCCA 
CTGTCCCTGC CCTGCTGCCT GCGCATTCCC AGCAAAGATC CTCTGGAGGA GGTCCCAGAG 
GCCCCAGTGC CCAGCAACAT GAGTACAGCA GCAGACCTCC TGCGTCAAGG CGCCGCCTGC 
AGCGTGCTCT ACCTGACCTC AGTGGAGACG GAGTCACTGA CGGGCCCCCA AGCAGTGGCA 
CGGGCCAGCT CCGCAGCTCT GAGCTGCAGC CCCCGCCCCA CACCAGCCGT TGTCCACTTC 
AAGGTCTCAG CCCAGGGCAT CACACTCACA GACAACCAAA GGAAGCTCTT CTTTCGCCGC 
CATTATCCAG TCAACAGCAT CACCTTTTCC AGCACTGACC CTCAGGACCG GAGATGGACC 
AACTCCGACG GGACCACCTC CAAGATCTTT GGTTTTGTGG CCAAGAAGCC GGGAAGCCCT 
TGGGAGAACG TGTGTCACCT CTTTGCAGAG CTTGACCCAG ATCAGCCCGC AGGCGCCATT 
GTCACCTTCA TCACCAAAGT TTTACTGGGC CAGAGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_024992420.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848188.1 Bos taurus tensin 2 (TNS2), transcript variant X7, mRNA.

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CloneID	OBa234738
Clone ID Related Accession (Same CDS sequence)	XM_024992420.1
Accession Version	XM_024992420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGATCAC CCCAGACCAC GGAAGAGGGG GTTCAGCCTT CCGTGCCAAG ATGGGTTGCC 
CTCTGCGTAG TTAAGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848188.1
CDS	215..4444
Translation	MGSPQTTEEGVQPSVPRWVALCVVKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_024992420.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992420.1

ORF Insert Sequence:

ATGGGATCAC CCCAGACCAC GGAAGAGGGG GTTCAGCCTT CCGTGCCAAG ATGGGTTGCC 
CTCTGCGTAG TTAAGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_024992421.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848189.1 Bos taurus tensin 2 (TNS2), transcript variant X8, mRNA.

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CloneID	OBa234739
Clone ID Related Accession (Same CDS sequence)	XM_024992421.1
Accession Version	XM_024992421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4230bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGCTGGC CCGGGGGCTC CCCCTGTTGC TGCCCCGCCC CGCCGCGCCC CCGCCCCGCC 
GGGCGCCCCC CGCAGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848189.1
CDS	77..4306
Translation	MGWPGGSPCCCPAPPRPRPAGRPPQPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_024992421.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992421.1

ORF Insert Sequence:

ATGGGCTGGC CCGGGGGCTC CCCCTGTTGC TGCCCCGCCC CGCCGCGCCC CCGCCCCGCC 
GGGCGCCCCC CGCAGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGGCA GCCTGTCAGC CCTGGTCTCC CAGCACTCCA TCTCCCCACT GTCCCTGCCC 
TGCTGCCTGC GCATTCCCAG CAAAGATCCT CTGGAGGAGG TCCCAGAGGC CCCAGTGCCC 
AGCAACATGA GTACAGCAGC AGACCTCCTG CGTCAAGGCG CCGCCTGCAG CGTGCTCTAC 
CTGACCTCAG TGGAGACGGA GTCACTGACG GGCCCCCAAG CAGTGGCACG GGCCAGCTCC 
GCAGCTCTGA GCTGCAGCCC CCGCCCCACA CCAGCCGTTG TCCACTTCAA GGTCTCAGCC 
CAGGGCATCA CACTCACAGA CAACCAAAGG AAGCTCTTCT TTCGCCGCCA TTATCCAGTC 
AACAGCATCA CCTTTTCCAG CACTGACCCT CAGGACCGGA GATGGACCAA CTCCGACGGG 
ACCACCTCCA AGATCTTTGG TTTTGTGGCC AAGAAGCCGG GAAGCCCTTG GGAGAACGTG 
TGTCACCTCT TTGCAGAGCT TGACCCAGAT CAGCCCGCAG GCGCCATTGT CACCTTCATC 
ACCAAAGTTT TACTGGGCCA GAGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_024992422.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848190.1 Bos taurus tensin 2 (TNS2), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OBa234740
Clone ID Related Accession (Same CDS sequence)	XM_024992422.1
Accession Version	XM_024992422.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGGCAGCC TGTCAGCCCT GGTCTCCCAG 
CACTCCATCT CCCCACTGTC CCTGCCCTGC TGCCTGCGCA TTCCCAGCAA AGCCTGCAGC 
GTGCTCTACC TGACCTCAGT GGAGACGGAG TCACTGACGG GCCCCCAAGC AGTGGCACGG 
GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC CGCCCCACAC CAGCCGTTGT CCACTTCAAG 
GTCTCAGCCC AGGGCATCAC ACTCACAGAC AACCAAAGGA AGCTCTTCTT TCGCCGCCAT 
TATCCAGTCA ACAGCATCAC CTTTTCCAGC ACTGACCCTC AGGACCGGAG ATGGACCAAC 
TCCGACGGGA CCACCTCCAA GATCTTTGGT TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG 
GAGAACGTGT GTCACCTCTT TGCAGAGCTT GACCCAGATC AGCCCGCAGG CGCCATTGTC 
ACCTTCATCA CCAAAGTTTT ACTGGGCCAG AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848190.1
CDS	5..4183
Translation	MCWEGGLLSSPPAQGQLLRKESGAGGAMKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_024992422.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992422.1

ORF Insert Sequence:

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGGCAGCC TGTCAGCCCT GGTCTCCCAG 
CACTCCATCT CCCCACTGTC CCTGCCCTGC TGCCTGCGCA TTCCCAGCAA AGCCTGCAGC 
GTGCTCTACC TGACCTCAGT GGAGACGGAG TCACTGACGG GCCCCCAAGC AGTGGCACGG 
GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC CGCCCCACAC CAGCCGTTGT CCACTTCAAG 
GTCTCAGCCC AGGGCATCAC ACTCACAGAC AACCAAAGGA AGCTCTTCTT TCGCCGCCAT 
TATCCAGTCA ACAGCATCAC CTTTTCCAGC ACTGACCCTC AGGACCGGAG ATGGACCAAC 
TCCGACGGGA CCACCTCCAA GATCTTTGGT TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG 
GAGAACGTGT GTCACCTCTT TGCAGAGCTT GACCCAGATC AGCCCGCAGG CGCCATTGTC 
ACCTTCATCA CCAAAGTTTT ACTGGGCCAG AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_024992423.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848191.1 Bos taurus tensin 2 (TNS2), transcript variant X10, mRNA.

Price & Availability↑

CloneID	OBa234741
Clone ID Related Accession (Same CDS sequence)	XM_024992423.1
Accession Version	XM_024992423.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4179bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGATCCTC TGGAGGAGGT CCCAGAGGCC 
CCAGTGCCCA GCAACATGAG TACAGCAGCA GACCTCCTGC GTCAAGGCGC CGCCTGCAGC 
GTGCTCTACC TGACCTCAGT GGAGACGGAG TCACTGACGG GCCCCCAAGC AGTGGCACGG 
GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC CGCCCCACAC CAGCCGTTGT CCACTTCAAG 
GTCTCAGCCC AGGGCATCAC ACTCACAGAC AACCAAAGGA AGCTCTTCTT TCGCCGCCAT 
TATCCAGTCA ACAGCATCAC CTTTTCCAGC ACTGACCCTC AGGACCGGAG ATGGACCAAC 
TCCGACGGGA CCACCTCCAA GATCTTTGGT TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG 
GAGAACGTGT GTCACCTCTT TGCAGAGCTT GACCCAGATC AGCCCGCAGG CGCCATTGTC 
ACCTTCATCA CCAAAGTTTT ACTGGGCCAG AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848191.1
CDS	5..4183
Translation	MCWEGGLLSSPPAQGQLLRKESGAGGAMKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_024992423.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992423.1

ORF Insert Sequence:

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGATCCTC TGGAGGAGGT CCCAGAGGCC 
CCAGTGCCCA GCAACATGAG TACAGCAGCA GACCTCCTGC GTCAAGGCGC CGCCTGCAGC 
GTGCTCTACC TGACCTCAGT GGAGACGGAG TCACTGACGG GCCCCCAAGC AGTGGCACGG 
GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC CGCCCCACAC CAGCCGTTGT CCACTTCAAG 
GTCTCAGCCC AGGGCATCAC ACTCACAGAC AACCAAAGGA AGCTCTTCTT TCGCCGCCAT 
TATCCAGTCA ACAGCATCAC CTTTTCCAGC ACTGACCCTC AGGACCGGAG ATGGACCAAC 
TCCGACGGGA CCACCTCCAA GATCTTTGGT TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG 
GAGAACGTGT GTCACCTCTT TGCAGAGCTT GACCCAGATC AGCCCGCAGG CGCCATTGTC 
ACCTTCATCA CCAAAGTTTT ACTGGGCCAG AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_024992424.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848192.1 Bos taurus tensin 2 (TNS2), transcript variant X11, mRNA.

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CloneID	OBa234742
Clone ID Related Accession (Same CDS sequence)	XM_024992424.1
Accession Version	XM_024992424.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4152bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGATC CTCTGGAGGA GGTCCCAGAG GCCCCAGTGC CCAGCAACAT GAGTACAGCA 
GCAGACCTCC TGCGTCAAGG CGCCGCCTGC AGCGTGCTCT ACCTGACCTC AGTGGAGACG 
GAGTCACTGA CGGGCCCCCA AGCAGTGGCA CGGGCCAGCT CCGCAGCTCT GAGCTGCAGC 
CCCCGCCCCA CACCAGCCGT TGTCCACTTC AAGGTCTCAG CCCAGGGCAT CACACTCACA 
GACAACCAAA GGAAGCTCTT CTTTCGCCGC CATTATCCAG TCAACAGCAT CACCTTTTCC 
AGCACTGACC CTCAGGACCG GAGATGGACC AACTCCGACG GGACCACCTC CAAGATCTTT 
GGTTTTGTGG CCAAGAAGCC GGGAAGCCCT TGGGAGAACG TGTGTCACCT CTTTGCAGAG 
CTTGACCCAG ATCAGCCCGC AGGCGCCATT GTCACCTTCA TCACCAAAGT TTTACTGGGC 
CAGAGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848192.1
CDS	339..4490
Translation	MKSSGPVERLLRALGRRDSSRATSRPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_024992424.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992424.1

ORF Insert Sequence:

ATGAAGTCCA GCGGCCCAGT GGAGAGGCTG CTGAGAGCCC TGGGGAGGAG GGACAGCAGC 
CGGGCCACCA GCAGGCCTAG GAAAGCTGAG CCGCATAGCT TCCGAGAGAA GGTCTTCCGG 
AAGAAACCAC CCGTGTGTGC CGTGTGTAAG GCAGCCATTG ATGGGACGGG CGTCTCATGC 
AGAGTCTGCA AGGTGGCAAC ACACAGAAAA TGTGAAGCAA AGGTGACTTC GTCCTGTCAG 
GCTTTGCCTC CCGTGGAGCT GCGGAGAAAC ACCGCCCCTG TGAGGCGCAT AGAGCACCTG 
GGATCCACCA AGTCTCTGAA CCACTCAAAG CAGCGCAGCA CTCTGCCCAG GAGCTTCAGC 
CTGGACCCGC TCATGGAGCG CCGCTGGGAC TTGGACCTCA CCTACGTGAC GGAGCGGATC 
CTTGCCGCCT CTTTCCCCGC GCGGCCTGAC GAGCAGCGAC ACCGGGGCCA CCTGCGCGAG 
CTGGCTCACG TGCTGCAATC CAAGCACCGT GACAAGTACC TGCTCTTCAA CCTTTCAGAG 
AAAAGACATG ACCTGACCCG CCTAAACCCG AAGGTCCAGG ACTTTGGCTG GCCTGAGCTG 
CACGCACCCC CCCTAGACAA GCTGTGCTCC ATTTGCAAAG CCATGGAGAC CTGGCTCAGT 
GCTGACCCGC AGCACGTGGT CGTATTGTAC TGCAAGGGGA GCAAGGGCAA GCTCGGGGTC 
ATCGTCTCTG CCTACATGCA CTACAGCAAG ATCTCTGCAG GGGCAGACCA GGCACTGGCG 
ACCCTTACCA TGCGGAAGTT CTGTGAAGAC AAGGTTGCCG CAGAATTGCA GCCCTCTCAG 
CGCCGGTATA TCACCTACTT CAGCGGTCTG CTGTCCGGGT CCATCCGCAT GAACAGCAGC 
CCTCTCTTCC TGCACCACGT GCTGGTGCCC GTGCTGCCAG CCTTTGAACC TGGTGCAGGA 
TTCCAGCCCT TCCTCAAGAT CTACCAGTCC ATGCAGCTTG TTTACACATC AGGAATCTAT 
CACATTGCAG GCCCTGGTCC CCAGCAGCTT TGTATCAGCC TGGAGCCAGC CCTCCTCCTC 
AAAGGCGATG TCATGGTAAC ATGCTATCAC AAGGGTAGCC GGGGAACTGA CCGGACCCTC 
GTATTCCGAG TCCAGTTCCA CACATGCACC ATCCATGGAC CACGGCTCAC CTTCTCCAAG 
GACCAGTTGG ATGAGGCCTG GACCGACGAG AGGTTCCCCT TCCAAGCCTC GGTGGAGTTC 
GTCTTCTCCT CCAGCCCAGA GAAGATCAAA GGTAACACCC CACAGAATGA GCCCTCCGTC 
TCTGTCGACT ACAACACTGC AGAGCCCGCA GTGCGCTGGG ACTCCTACGA GAACTTCAAC 
TTGCACCATG AGGACAGTGC GGATGACTCT GTCACCCACA CCCGGGGGCC CCTGGATGGC 
AGTCCTTATG CCCAGGTGCA GCGAGCCCCC CGCCAGACCC CGCCGGCCCC GTCTCCGGAG 
CCACCCCCAC CCCCGCTGCT TTCTGTCAGC AGCGACTCTG GCCACTCCTC CACACTGACC 
ACAGAGCCAG CTGCCGAGTC CCCTGGCCGG CCACCCCCGA CGGCTGCCGA GCGGCAGGAG 
CTCGAGCGCC TACTGGGAGG CTGTGGAGTG GCTAGTGGGG GCCGGGGAGC TGGGCGCGAG 
ACGGCCATCC TTGATGACGA AGACCAGCCT GCTGCAGGCG GAGGCCCCCA CCTCGGAATA 
TATTCGGGAC ACAGGCCTGG CCTCAGCCGC CACTGCTCCT GCCGCCAGGG CTACCGGGAA 
CCCTGCGGGG TCCCCAATGG AGGTTACTAC CGGCCAGAGG GGACCCTGGA GAGGCGGCGG 
CTGGCTTTCG GAGCCTATGA GGGGCCCCCC CAGGGTTATG CTGAGCCCTC CGTGGAGAAG 
AGACGCCTCT GCCGCTCGCT GTCCGAGGGG CCGTACCCCT ACCCGTCTGA GCTGGGGAAA 
CCCGCCAATG GAGACTTTGG CTACCGCCCC CCAGGCTACC GGGAGGTGGT GATCCTGGAA 
GACCCGGGGC TGCCTCCGCT GTGCTCGTGC CCCGCCTGTG AGGAGAAGCT AGCACTGCCC 
ACGGCAGCCC TCTATGGGCT GCGGCTGGAG AGGGAGGCTG GAGAGGGGTG GGCAAGTGAG 
GCTGGCAAGC CCCTCCTGCA CCCAGTGCGA CCCGGGCACC CGCTGCCCCT GCTGGTGCCT 
GCCTGTGGGC ACCACCACAC CCCGCTGCCT GACTACAGCT GCTTGAAGCC ACCCAAGGCA 
GGCGAGGAAG GGCATGAGGG CTGCTCCTAC GCCTTGTGCC CCGAGGGAAG GTATGGGCAC 
CCAGGGTACC CTGCCCTGGT GACGTACGGC TATGGAGGAG CAGTTCCCAG TTACTGCCCA 
GCATATGGCC GGGTGCCGCA CAGCTGTGGG TCTCCAGGTG AGGGCAGAGG GTATCCCAGC 
CCTCGTGCCC ACTCCCCCCG GGCTGGCTCC ATTTCCCCGG GCAGCCCGCC CTACCCCCAG 
TCCAGGAAGC TGAGCTACGA GATCCCTGCA GAGGAGGGAG GGGACCGGTA TCTGCTGCCT 
GGGCACCTGG CCCCAGCAGG ACCCTTGGCA TCTGCAGAGT CGCCAGAGCC TGTGTCCTGG 
AGGGAGGGTC CCAGCGGGCA CAGCACCCTG CCCCGGTCCC CGCGAGATGC CCAGTGCAGC 
GCTGCTTCCG AGCTGTCTGG TCCTTCCACG CCGCTGCACA CCAGCAGCCC CGTCCAGGGC 
AAGGAGAGCG CCCGACGGCA GGACACCCGC TCCCCCACCT TGGCGCCCAC TCAGAGACTG 
AGTCCTGCGG AGGCCTTGCC ACCTGTTTCC CAGGGAGGCG CTGATAAGGC TCCAGAGCTG 
CCTGCAAGAA GTGGGCCTGA GCCTCCAGCC CCTGGCGCCT TCTCCCTAGC CTCCCCACCC 
AGCTCTCCCA ACGACTGGCC TCAGGAGAGG AGCCCGGGGG GCCGCTCAGA CAGCGCCAGT 
CCAAGGGGGC CTGTACCCAA CACCCTGCCC GGCCTCCGTC ATGCCCCCTG GCAGGGCCTG 
CGAGACCCCC CGGACAGCCC AGATGGGTCC CCCCTCACCC CTGTGCCTAC TCAGATGCCC 
TGGCTTGTGG CCAGCCCAGA ACCCCCTCAG AGCTCGCCCA CACCTGCCTT TCCTCTGGCT 
GCATCTTACG ACATCAACGG CGCCCCCCAG CCCCCTCTTC CTGAGAAACG CCACCTGCTG 
GGGCCTGGGC AGCAGCCGGG ACCCTGGGGC CCAGAGCAGG CGTCCCCACC AGCCAGAGGC 
GCTAGTCACC ATGTCACTTT TGCACCTCTG CTCCCGGATA ACACCCCCCA GCCCCCAGAG 
CCCCCGATGC AAGAGAGCCA GAGCAATGTC AAGTTTGTCC AGGACACATC CAAGTTCTGG 
TACAAGCCGC ACCTGTCCCG TGACCAAGCC ATCACCCTGC TGAAGGACAA AGACCCTGGG 
GCCTTCCTGA TCAGGGACAG TCATTCATTC CAAGGAGCCT ATGGGCTGGC TCTCAAGGTG 
GCCACGCCCC CTCCCAGCGC CCAGTCCTGG AAAGGGGACC CCTCAGAACA GCTGGTCCGT 
CATTTTCTCA TTGAGACTGG GCCGAAAGGG GTGAAGATCA AGGGCTGTCC CAGCGAGCCC 
TACTTTGATC CTCTGGAGGA GGTCCCAGAG GCCCCAGTGC CCAGCAACAT GAGTACAGCA 
GCAGACCTCC TGCGTCAAGG CGCCGCCTGC AGCGTGCTCT ACCTGACCTC AGTGGAGACG 
GAGTCACTGA CGGGCCCCCA AGCAGTGGCA CGGGCCAGCT CCGCAGCTCT GAGCTGCAGC 
CCCCGCCCCA CACCAGCCGT TGTCCACTTC AAGGTCTCAG CCCAGGGCAT CACACTCACA 
GACAACCAAA GGAAGCTCTT CTTTCGCCGC CATTATCCAG TCAACAGCAT CACCTTTTCC 
AGCACTGACC CTCAGGACCG GAGATGGACC AACTCCGACG GGACCACCTC CAAGATCTTT 
GGTTTTGTGG CCAAGAAGCC GGGAAGCCCT TGGGAGAACG TGTGTCACCT CTTTGCAGAG 
CTTGACCCAG ATCAGCCCGC AGGCGCCATT GTCACCTTCA TCACCAAAGT TTTACTGGGC 
CAGAGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_024992425.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024848193.1 Bos taurus tensin 2 (TNS2), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OBa234743
Clone ID Related Accession (Same CDS sequence)	XM_024992425.1
Accession Version	XM_024992425.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGAGTCG CCAGAGCCTG TGTCCTGGAG GGAGGGTCCC 
AGCGGGCACA GCACCCTGCC CCGGTCCCCG CGAGATGCCC AGTGCAGCGC TGCTTCCGAG 
CTGTCTGGTC CTTCCACGCC GCTGCACACC AGCAGCCCCG TCCAGGGCAA GGAGAGCGCC 
CGACGGCAGG ACACCCGCTC CCCCACCTTG GCGCCCACTC AGAGACTGAG TCCTGCGGAG 
GCCTTGCCAC CTGTTTCCCA GGGAGGCGCT GATAAGGCTC CAGAGCTGCC TGCAAGAAGT 
GGGCCTGAGC CTCCAGCCCC TGGCGCCTTC TCCCTAGCCT CCCCACCCAG CTCTCCCAAC 
GACTGGCCTC AGGAGAGGAG CCCGGGGGGC CGCTCAGACA GCGCCAGTCC AAGGGGGCCT 
GTACCCAACA CCCTGCCCGG CCTCCGTCAT GCCCCCTGGC AGGGCCTGCG AGACCCCCCG 
GACAGCCCAG ATGGGTCCCC CCTCACCCCT GTGCCTACTC AGATGCCCTG GCTTGTGGCC 
AGCCCAGAAC CCCCTCAGAG CTCGCCCACA CCTGCCTTTC CTCTGGCTGC ATCTTACGAC 
ATCAACGGCG CCCCCCAGCC CCCTCTTCCT GAGAAACGCC ACCTGCTGGG GCCTGGGCAG 
CAGCCGGGAC CCTGGGGCCC AGAGCAGGCG TCCCCACCAG CCAGAGGCGC TAGTCACCAT 
GTCACTTTTG CACCTCTGCT CCCGGATAAC ACCCCCCAGC CCCCAGAGCC CCCGATGCAA 
GAGAGCCAGA GCAATGTCAA GTTTGTCCAG GACACATCCA AGTTCTGGTA CAAGCCGCAC 
CTGTCCCGTG ACCAAGCCAT CACCCTGCTG AAGGACAAAG ACCCTGGGGC CTTCCTGATC 
AGGGACAGTC ATTCATTCCA AGGAGCCTAT GGGCTGGCTC TCAAGGTGGC CACGCCCCCT 
CCCAGCGCCC AGTCCTGGAA AGGGGACCCC TCAGAACAGC TGGTCCGTCA TTTTCTCATT 
GAGACTGGGC CGAAAGGGGT GAAGATCAAG GGCTGTCCCA GCGAGCCCTA CTTTGGCAGC 
CTGTCAGCCC TGGTCTCCCA GCACTCCATC TCCCCACTGT CCCTGCCCTG CTGCCTGCGC 
ATTCCCAGCA AAGATCCTCT GGAGGAGGTC CCAGAGGCCC CAGTGCCCAG CAACATGAGT 
ACAGCAGCAG ACCTCCTGCG TCAAGGCGCC GCCTGCAGCG TGCTCTACCT GACCTCAGTG 
GAGACGGAGT CACTGACGGG CCCCCAAGCA GTGGCACGGG CCAGCTCCGC AGCTCTGAGC 
TGCAGCCCCC GCCCCACACC AGCCGTTGTC CACTTCAAGG TCTCAGCCCA GGGCATCACA 
CTCACAGACA ACCAAAGGAA GCTCTTCTTT CGCCGCCATT ATCCAGTCAA CAGCATCACC 
TTTTCCAGCA CTGACCCTCA GGACCGGAGA TGGACCAACT CCGACGGGAC CACCTCCAAG 
ATCTTTGGTT TTGTGGCCAA GAAGCCGGGA AGCCCTTGGG AGAACGTGTG TCACCTCTTT 
GCAGAGCTTG ACCCAGATCA GCCCGCAGGC GCCATTGTCA CCTTCATCAC CAAAGTTTTA 
CTGGGCCAGA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024848193.1
CDS	787..4644
Translation	MERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_024992425.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024992425.1

ORF Insert Sequence:

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGAGTCG CCAGAGCCTG TGTCCTGGAG GGAGGGTCCC 
AGCGGGCACA GCACCCTGCC CCGGTCCCCG CGAGATGCCC AGTGCAGCGC TGCTTCCGAG 
CTGTCTGGTC CTTCCACGCC GCTGCACACC AGCAGCCCCG TCCAGGGCAA GGAGAGCGCC 
CGACGGCAGG ACACCCGCTC CCCCACCTTG GCGCCCACTC AGAGACTGAG TCCTGCGGAG 
GCCTTGCCAC CTGTTTCCCA GGGAGGCGCT GATAAGGCTC CAGAGCTGCC TGCAAGAAGT 
GGGCCTGAGC CTCCAGCCCC TGGCGCCTTC TCCCTAGCCT CCCCACCCAG CTCTCCCAAC 
GACTGGCCTC AGGAGAGGAG CCCGGGGGGC CGCTCAGACA GCGCCAGTCC AAGGGGGCCT 
GTACCCAACA CCCTGCCCGG CCTCCGTCAT GCCCCCTGGC AGGGCCTGCG AGACCCCCCG 
GACAGCCCAG ATGGGTCCCC CCTCACCCCT GTGCCTACTC AGATGCCCTG GCTTGTGGCC 
AGCCCAGAAC CCCCTCAGAG CTCGCCCACA CCTGCCTTTC CTCTGGCTGC ATCTTACGAC 
ATCAACGGCG CCCCCCAGCC CCCTCTTCCT GAGAAACGCC ACCTGCTGGG GCCTGGGCAG 
CAGCCGGGAC CCTGGGGCCC AGAGCAGGCG TCCCCACCAG CCAGAGGCGC TAGTCACCAT 
GTCACTTTTG CACCTCTGCT CCCGGATAAC ACCCCCCAGC CCCCAGAGCC CCCGATGCAA 
GAGAGCCAGA GCAATGTCAA GTTTGTCCAG GACACATCCA AGTTCTGGTA CAAGCCGCAC 
CTGTCCCGTG ACCAAGCCAT CACCCTGCTG AAGGACAAAG ACCCTGGGGC CTTCCTGATC 
AGGGACAGTC ATTCATTCCA AGGAGCCTAT GGGCTGGCTC TCAAGGTGGC CACGCCCCCT 
CCCAGCGCCC AGTCCTGGAA AGGGGACCCC TCAGAACAGC TGGTCCGTCA TTTTCTCATT 
GAGACTGGGC CGAAAGGGGT GAAGATCAAG GGCTGTCCCA GCGAGCCCTA CTTTGGCAGC 
CTGTCAGCCC TGGTCTCCCA GCACTCCATC TCCCCACTGT CCCTGCCCTG CTGCCTGCGC 
ATTCCCAGCA AAGATCCTCT GGAGGAGGTC CCAGAGGCCC CAGTGCCCAG CAACATGAGT 
ACAGCAGCAG ACCTCCTGCG TCAAGGCGCC GCCTGCAGCG TGCTCTACCT GACCTCAGTG 
GAGACGGAGT CACTGACGGG CCCCCAAGCA GTGGCACGGG CCAGCTCCGC AGCTCTGAGC 
TGCAGCCCCC GCCCCACACC AGCCGTTGTC CACTTCAAGG TCTCAGCCCA GGGCATCACA 
CTCACAGACA ACCAAAGGAA GCTCTTCTTT CGCCGCCATT ATCCAGTCAA CAGCATCACC 
TTTTCCAGCA CTGACCCTCA GGACCGGAGA TGGACCAACT CCGACGGGAC CACCTCCAAG 
ATCTTTGGTT TTGTGGCCAA GAAGCCGGGA AGCCCTTGGG AGAACGTGTG TCACCTCTTT 
GCAGAGCTTG ACCCAGATCA GCCCGCAGGC GCCATTGTCA CCTTCATCAC CAAAGTTTTA 
CTGGGCCAGA GGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_015463275.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318761.1 Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa89855
Clone ID Related Accession (Same CDS sequence)	XM_015463275.1 , XM_015470962.1
Accession Version	XM_015463275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644788.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GCTCCTCTCC CTGCAGAGTC GCCAGAGCCT GTGTCCTGGA GGGAGGGTCC CAGCGGGCAC 
AGCACCCTGC CCCGGTCCCC GCGAGATGCC CAGTGCAGCG CTGCTTCCGA GCTGTCTGGT 
CCTTCCACGC CGCTGCACAC CAGCAGCCCC GTCCAGGGCA AGGAGAGCGC CCGACGGCAG 
GACACCCGCT CCCCCACCTT GGCGCCCACT CAGAGACTGA GTCCTGCGGA GGCCTTGCCA 
CCTGTTTCCC AGGGAGGCGC TGATAAGGCT CCAGAGCTGC CTGCAAGAAG TGGGCCTGAG 
CCTCCAGCCC CTGGCGCCTT CTCCCTAGCC TCCCCACCCA GCTCTCCCAA CGACTGGCCT 
CAGGAGAGGA GCCCGGGGGG CCGCTCAGAC AGCGCCAGTC CAAGGGGGCC TGTACCCAAC 
ACCCTGCCCG GCCTCCGTCA TGCCCCCTGG CAGGGCCTGC GAGACCCCCC GGACAGCCCA 
GATGGGTCCC CCCTCACCCC TGTGCCTACT CAGATGCCCT GGCTTGTGGC CAGCCCAGAA 
CCCCCTCAGA GCTCGCCCAC ACCTGCCTTT CCTCTGGCTG CATCTTACGA CATCAACGGC 
GCCCCCCAGC CCCCTCTTCC TGAGAAACGC CACCTGCTGG GGCCTGGGCA GCAGCCGGGA 
CCCTGGGGCC CAGAGCAGGC GTCCCCACCA GCCAGAGGCG CTAGTCACCA TGTCACTTTT 
GCACCTCTGC TCCCGGATAA CACCCCCCAG CCCCCAGAGC CCCCGATGCA AGAGAGCCAG 
AGCAATGTCA AGTTTGTCCA GGACACATCC AAGTTCTGGT ACAAGCCGCA CCTGTCCCGT 
GACCAAGCCA TCACCCTGCT GAAGGACAAA GACCCTGGGG CCTTCCTGAT CAGGGACAGT 
CATTCATTCC AAGGAGCCTA TGGGCTGGCT CTCAAGGTGG CCACGCCCCC TCCCAGCGCC 
CAGTCCTGGA AAGGGGACCC CTCAGAACAG CTGGTCCGTC ATTTTCTCAT TGAGACTGGG 
CCGAAAGGGG TGAAGATCAA GGGCTGTCCC AGCGAGCCCT ACTTTGGCAG CCTGTCAGCC 
CTGGTCTCCC AGCACTCCAT CTCCCCACTG TCCCTGCCCT GCTGCCTGCG CATTCCCAGC 
AAAGATCCTC TGGAGGAGGT CCCAGAGGCC CCAGTGCCCA GCAACATGAG TACAGCAGCA 
GACCTCCTGC GTCAAGGCGC CGCCTGCAGC GTGCTCTACC TGACCTCAGT GGAGACGGAG 
TCACTGACGG GCCCCCAAGC AGTGGCACGG GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC 
CGCCCCACAC CAGCCGTTGT CCACTTCAAG GTCTCAGCCC AGGGCATCAC ACTCACAGAC 
AACCAAAGGA AGCTCTTCTT TCGCCGCCAT TATCCAGTCA ACAGCATCAC CTTTTCCAGC 
ACTGACCCTC AGGACCGGAG ATGGACCAAC TCCGACGGGA CCACCTCCAA GATCTTTGGT 
TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG GAGAACGTGT GTCACCTCTT TGCAGAGCTT 
GACCCAGATC AGCCCGCAGG CGCCATTGTC ACCTTCATCA CCAAAGTTTT ACTGGGCCAG 
AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318761.1
CDS	1394..5662
Translation	MENRKSAALRPGPVLEMDPWELTPLSVCPSTRQPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_015463275.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463275.1

ORF Insert Sequence:

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GCTCCTCTCC CTGCAGAGTC GCCAGAGCCT GTGTCCTGGA GGGAGGGTCC CAGCGGGCAC 
AGCACCCTGC CCCGGTCCCC GCGAGATGCC CAGTGCAGCG CTGCTTCCGA GCTGTCTGGT 
CCTTCCACGC CGCTGCACAC CAGCAGCCCC GTCCAGGGCA AGGAGAGCGC CCGACGGCAG 
GACACCCGCT CCCCCACCTT GGCGCCCACT CAGAGACTGA GTCCTGCGGA GGCCTTGCCA 
CCTGTTTCCC AGGGAGGCGC TGATAAGGCT CCAGAGCTGC CTGCAAGAAG TGGGCCTGAG 
CCTCCAGCCC CTGGCGCCTT CTCCCTAGCC TCCCCACCCA GCTCTCCCAA CGACTGGCCT 
CAGGAGAGGA GCCCGGGGGG CCGCTCAGAC AGCGCCAGTC CAAGGGGGCC TGTACCCAAC 
ACCCTGCCCG GCCTCCGTCA TGCCCCCTGG CAGGGCCTGC GAGACCCCCC GGACAGCCCA 
GATGGGTCCC CCCTCACCCC TGTGCCTACT CAGATGCCCT GGCTTGTGGC CAGCCCAGAA 
CCCCCTCAGA GCTCGCCCAC ACCTGCCTTT CCTCTGGCTG CATCTTACGA CATCAACGGC 
GCCCCCCAGC CCCCTCTTCC TGAGAAACGC CACCTGCTGG GGCCTGGGCA GCAGCCGGGA 
CCCTGGGGCC CAGAGCAGGC GTCCCCACCA GCCAGAGGCG CTAGTCACCA TGTCACTTTT 
GCACCTCTGC TCCCGGATAA CACCCCCCAG CCCCCAGAGC CCCCGATGCA AGAGAGCCAG 
AGCAATGTCA AGTTTGTCCA GGACACATCC AAGTTCTGGT ACAAGCCGCA CCTGTCCCGT 
GACCAAGCCA TCACCCTGCT GAAGGACAAA GACCCTGGGG CCTTCCTGAT CAGGGACAGT 
CATTCATTCC AAGGAGCCTA TGGGCTGGCT CTCAAGGTGG CCACGCCCCC TCCCAGCGCC 
CAGTCCTGGA AAGGGGACCC CTCAGAACAG CTGGTCCGTC ATTTTCTCAT TGAGACTGGG 
CCGAAAGGGG TGAAGATCAA GGGCTGTCCC AGCGAGCCCT ACTTTGGCAG CCTGTCAGCC 
CTGGTCTCCC AGCACTCCAT CTCCCCACTG TCCCTGCCCT GCTGCCTGCG CATTCCCAGC 
AAAGATCCTC TGGAGGAGGT CCCAGAGGCC CCAGTGCCCA GCAACATGAG TACAGCAGCA 
GACCTCCTGC GTCAAGGCGC CGCCTGCAGC GTGCTCTACC TGACCTCAGT GGAGACGGAG 
TCACTGACGG GCCCCCAAGC AGTGGCACGG GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC 
CGCCCCACAC CAGCCGTTGT CCACTTCAAG GTCTCAGCCC AGGGCATCAC ACTCACAGAC 
AACCAAAGGA AGCTCTTCTT TCGCCGCCAT TATCCAGTCA ACAGCATCAC CTTTTCCAGC 
ACTGACCCTC AGGACCGGAG ATGGACCAAC TCCGACGGGA CCACCTCCAA GATCTTTGGT 
TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG GAGAACGTGT GTCACCTCTT TGCAGAGCTT 
GACCCAGATC AGCCCGCAGG CGCCATTGTC ACCTTCATCA CCAAAGTTTT ACTGGGCCAG 
AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_015470962.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326448.1 Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.

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CloneID	OBa89855
Clone ID Related Accession (Same CDS sequence)	XM_015463275.1 , XM_015470962.1
Accession Version	XM_015470962.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4269bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103919.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GCTCCTCTCC CTGCAGAGTC GCCAGAGCCT GTGTCCTGGA GGGAGGGTCC CAGCGGGCAC 
AGCACCCTGC CCCGGTCCCC GCGAGATGCC CAGTGCAGCG CTGCTTCCGA GCTGTCTGGT 
CCTTCCACGC CGCTGCACAC CAGCAGCCCC GTCCAGGGCA AGGAGAGCGC CCGACGGCAG 
GACACCCGCT CCCCCACCTT GGCGCCCACT CAGAGACTGA GTCCTGCGGA GGCCTTGCCA 
CCTGTTTCCC AGGGAGGCGC TGATAAGGCT CCAGAGCTGC CTGCAAGAAG TGGGCCTGAG 
CCTCCAGCCC CTGGCGCCTT CTCCCTAGCC TCCCCACCCA GCTCTCCCAA CGACTGGCCT 
CAGGAGAGGA GCCCGGGGGG CCGCTCAGAC AGCGCCAGTC CAAGGGGGCC TGTACCCAAC 
ACCCTGCCCG GCCTCCGTCA TGCCCCCTGG CAGGGCCTGC GAGACCCCCC GGACAGCCCA 
GATGGGTCCC CCCTCACCCC TGTGCCTACT CAGATGCCCT GGCTTGTGGC CAGCCCAGAA 
CCCCCTCAGA GCTCGCCCAC ACCTGCCTTT CCTCTGGCTG CATCTTACGA CATCAACGGC 
GCCCCCCAGC CCCCTCTTCC TGAGAAACGC CACCTGCTGG GGCCTGGGCA GCAGCCGGGA 
CCCTGGGGCC CAGAGCAGGC GTCCCCACCA GCCAGAGGCG CTAGTCACCA TGTCACTTTT 
GCACCTCTGC TCCCGGATAA CACCCCCCAG CCCCCAGAGC CCCCGATGCA AGAGAGCCAG 
AGCAATGTCA AGTTTGTCCA GGACACATCC AAGTTCTGGT ACAAGCCGCA CCTGTCCCGT 
GACCAAGCCA TCACCCTGCT GAAGGACAAA GACCCTGGGG CCTTCCTGAT CAGGGACAGT 
CATTCATTCC AAGGAGCCTA TGGGCTGGCT CTCAAGGTGG CCACGCCCCC TCCCAGCGCC 
CAGTCCTGGA AAGGGGACCC CTCAGAACAG CTGGTCCGTC ATTTTCTCAT TGAGACTGGG 
CCGAAAGGGG TGAAGATCAA GGGCTGTCCC AGCGAGCCCT ACTTTGGCAG CCTGTCAGCC 
CTGGTCTCCC AGCACTCCAT CTCCCCACTG TCCCTGCCCT GCTGCCTGCG CATTCCCAGC 
AAAGATCCTC TGGAGGAGGT CCCAGAGGCC CCAGTGCCCA GCAACATGAG TACAGCAGCA 
GACCTCCTGC GTCAAGGCGC CGCCTGCAGC GTGCTCTACC TGACCTCAGT GGAGACGGAG 
TCACTGACGG GCCCCCAAGC AGTGGCACGG GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC 
CGCCCCACAC CAGCCGTTGT CCACTTCAAG GTCTCAGCCC AGGGCATCAC ACTCACAGAC 
AACCAAAGGA AGCTCTTCTT TCGCCGCCAT TATCCAGTCA ACAGCATCAC CTTTTCCAGC 
ACTGACCCTC AGGACCGGAG ATGGACCAAC TCCGACGGGA CCACCTCCAA GATCTTTGGT 
TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG GAGAACGTGT GTCACCTCTT TGCAGAGCTT 
GACCCAGATC AGCCCGCAGG CGCCATTGTC ACCTTCATCA CCAAAGTTTT ACTGGGCCAG 
AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326448.1
CDS	1394..5662
Translation	MENRKSAALRPGPVLEMDPWELTPLSVCPSTRQPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_015470962.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470962.1

ORF Insert Sequence:

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GCTCCTCTCC CTGCAGAGTC GCCAGAGCCT GTGTCCTGGA GGGAGGGTCC CAGCGGGCAC 
AGCACCCTGC CCCGGTCCCC GCGAGATGCC CAGTGCAGCG CTGCTTCCGA GCTGTCTGGT 
CCTTCCACGC CGCTGCACAC CAGCAGCCCC GTCCAGGGCA AGGAGAGCGC CCGACGGCAG 
GACACCCGCT CCCCCACCTT GGCGCCCACT CAGAGACTGA GTCCTGCGGA GGCCTTGCCA 
CCTGTTTCCC AGGGAGGCGC TGATAAGGCT CCAGAGCTGC CTGCAAGAAG TGGGCCTGAG 
CCTCCAGCCC CTGGCGCCTT CTCCCTAGCC TCCCCACCCA GCTCTCCCAA CGACTGGCCT 
CAGGAGAGGA GCCCGGGGGG CCGCTCAGAC AGCGCCAGTC CAAGGGGGCC TGTACCCAAC 
ACCCTGCCCG GCCTCCGTCA TGCCCCCTGG CAGGGCCTGC GAGACCCCCC GGACAGCCCA 
GATGGGTCCC CCCTCACCCC TGTGCCTACT CAGATGCCCT GGCTTGTGGC CAGCCCAGAA 
CCCCCTCAGA GCTCGCCCAC ACCTGCCTTT CCTCTGGCTG CATCTTACGA CATCAACGGC 
GCCCCCCAGC CCCCTCTTCC TGAGAAACGC CACCTGCTGG GGCCTGGGCA GCAGCCGGGA 
CCCTGGGGCC CAGAGCAGGC GTCCCCACCA GCCAGAGGCG CTAGTCACCA TGTCACTTTT 
GCACCTCTGC TCCCGGATAA CACCCCCCAG CCCCCAGAGC CCCCGATGCA AGAGAGCCAG 
AGCAATGTCA AGTTTGTCCA GGACACATCC AAGTTCTGGT ACAAGCCGCA CCTGTCCCGT 
GACCAAGCCA TCACCCTGCT GAAGGACAAA GACCCTGGGG CCTTCCTGAT CAGGGACAGT 
CATTCATTCC AAGGAGCCTA TGGGCTGGCT CTCAAGGTGG CCACGCCCCC TCCCAGCGCC 
CAGTCCTGGA AAGGGGACCC CTCAGAACAG CTGGTCCGTC ATTTTCTCAT TGAGACTGGG 
CCGAAAGGGG TGAAGATCAA GGGCTGTCCC AGCGAGCCCT ACTTTGGCAG CCTGTCAGCC 
CTGGTCTCCC AGCACTCCAT CTCCCCACTG TCCCTGCCCT GCTGCCTGCG CATTCCCAGC 
AAAGATCCTC TGGAGGAGGT CCCAGAGGCC CCAGTGCCCA GCAACATGAG TACAGCAGCA 
GACCTCCTGC GTCAAGGCGC CGCCTGCAGC GTGCTCTACC TGACCTCAGT GGAGACGGAG 
TCACTGACGG GCCCCCAAGC AGTGGCACGG GCCAGCTCCG CAGCTCTGAG CTGCAGCCCC 
CGCCCCACAC CAGCCGTTGT CCACTTCAAG GTCTCAGCCC AGGGCATCAC ACTCACAGAC 
AACCAAAGGA AGCTCTTCTT TCGCCGCCAT TATCCAGTCA ACAGCATCAC CTTTTCCAGC 
ACTGACCCTC AGGACCGGAG ATGGACCAAC TCCGACGGGA CCACCTCCAA GATCTTTGGT 
TTTGTGGCCA AGAAGCCGGG AAGCCCTTGG GAGAACGTGT GTCACCTCTT TGCAGAGCTT 
GACCCAGATC AGCCCGCAGG CGCCATTGTC ACCTTCATCA CCAAAGTTTT ACTGGGCCAG 
AGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005206244.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206301.3 Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.

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CloneID	OBa89856
Clone ID Related Accession (Same CDS sequence)	XM_005206244.3 , XM_005198238.3
Accession Version	XM_005206244.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206244.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGGCAGCC TGTCAGCCCT GGTCTCCCAG 
CACTCCATCT CCCCACTGTC CCTGCCCTGC TGCCTGCGCA TTCCCAGCAA AGATCCTCTG 
GAGGAGGTCC CAGAGGCCCC AGTGCCCAGC AACATGAGTA CAGCAGCAGA CCTCCTGCGT 
CAAGGCGCCG CCTGCAGCGT GCTCTACCTG ACCTCAGTGG AGACGGAGTC ACTGACGGGC 
CCCCAAGCAG TGGCACGGGC CAGCTCCGCA GCTCTGAGCT GCAGCCCCCG CCCCACACCA 
GCCGTTGTCC ACTTCAAGGT CTCAGCCCAG GGCATCACAC TCACAGACAA CCAAAGGAAG 
CTCTTCTTTC GCCGCCATTA TCCAGTCAAC AGCATCACCT TTTCCAGCAC TGACCCTCAG 
GACCGGAGAT GGACCAACTC CGACGGGACC ACCTCCAAGA TCTTTGGTTT TGTGGCCAAG 
AAGCCGGGAA GCCCTTGGGA GAACGTGTGT CACCTCTTTG CAGAGCTTGA CCCAGATCAG 
CCCGCAGGCG CCATTGTCAC CTTCATCACC AAAGTTTTAC TGGGCCAGAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206301.3
CDS	4..4260
Translation	MCWEGGLLSSPPAQGQLLRKESGAGGAMKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005206244.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206244.3

ORF Insert Sequence:

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGGCAGCC TGTCAGCCCT GGTCTCCCAG 
CACTCCATCT CCCCACTGTC CCTGCCCTGC TGCCTGCGCA TTCCCAGCAA AGATCCTCTG 
GAGGAGGTCC CAGAGGCCCC AGTGCCCAGC AACATGAGTA CAGCAGCAGA CCTCCTGCGT 
CAAGGCGCCG CCTGCAGCGT GCTCTACCTG ACCTCAGTGG AGACGGAGTC ACTGACGGGC 
CCCCAAGCAG TGGCACGGGC CAGCTCCGCA GCTCTGAGCT GCAGCCCCCG CCCCACACCA 
GCCGTTGTCC ACTTCAAGGT CTCAGCCCAG GGCATCACAC TCACAGACAA CCAAAGGAAG 
CTCTTCTTTC GCCGCCATTA TCCAGTCAAC AGCATCACCT TTTCCAGCAC TGACCCTCAG 
GACCGGAGAT GGACCAACTC CGACGGGACC ACCTCCAAGA TCTTTGGTTT TGTGGCCAAG 
AAGCCGGGAA GCCCTTGGGA GAACGTGTGT CACCTCTTTG CAGAGCTTGA CCCAGATCAG 
CCCGCAGGCG CCATTGTCAC CTTCATCACC AAAGTTTTAC TGGGCCAGAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005198238.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198295.3 Bos taurus tensin 2 (TNS2), transcript variant X2, mRNA.

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CloneID	OBa89856
Clone ID Related Accession (Same CDS sequence)	XM_005206244.3 , XM_005198238.3
Accession Version	XM_005198238.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4257bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644788.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198238.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGGCAGCC TGTCAGCCCT GGTCTCCCAG 
CACTCCATCT CCCCACTGTC CCTGCCCTGC TGCCTGCGCA TTCCCAGCAA AGATCCTCTG 
GAGGAGGTCC CAGAGGCCCC AGTGCCCAGC AACATGAGTA CAGCAGCAGA CCTCCTGCGT 
CAAGGCGCCG CCTGCAGCGT GCTCTACCTG ACCTCAGTGG AGACGGAGTC ACTGACGGGC 
CCCCAAGCAG TGGCACGGGC CAGCTCCGCA GCTCTGAGCT GCAGCCCCCG CCCCACACCA 
GCCGTTGTCC ACTTCAAGGT CTCAGCCCAG GGCATCACAC TCACAGACAA CCAAAGGAAG 
CTCTTCTTTC GCCGCCATTA TCCAGTCAAC AGCATCACCT TTTCCAGCAC TGACCCTCAG 
GACCGGAGAT GGACCAACTC CGACGGGACC ACCTCCAAGA TCTTTGGTTT TGTGGCCAAG 
AAGCCGGGAA GCCCTTGGGA GAACGTGTGT CACCTCTTTG CAGAGCTTGA CCCAGATCAG 
CCCGCAGGCG CCATTGTCAC CTTCATCACC AAAGTTTTAC TGGGCCAGAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198295.3
CDS	4..4260
Translation	MCWEGGLLSSPPAQGQLLRKESGAGGAMKPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005198238.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005198238.3

ORF Insert Sequence:

ATGTGTTGGG AGGGGGGCCT CCTGTCCAGC CCTCCAGCCC AGGGACAGCT GCTGAGGAAG 
GAGAGCGGAG CTGGGGGAGC CATGAAGCCT AGGAAAGCTG AGCCGCATAG CTTCCGAGAG 
AAGGTCTTCC GGAAGAAACC ACCCGTGTGT GCCGTGTGTA AGGCAGCCAT TGATGGGACG 
GGCGTCTCAT GCAGAGTCTG CAAGGTGGCA ACACACAGAA AATGTGAAGC AAAGGTGACT 
TCGTCCTGTC AGGCTTTGCC TCCCGTGGAG CTGCGGAGAA ACACCGCCCC TGTGAGGCGC 
ATAGAGCACC TGGGATCCAC CAAGTCTCTG AACCACTCAA AGCAGCGCAG CACTCTGCCC 
AGGAGCTTCA GCCTGGACCC GCTCATGGAG CGCCGCTGGG ACTTGGACCT CACCTACGTG 
ACGGAGCGGA TCCTTGCCGC CTCTTTCCCC GCGCGGCCTG ACGAGCAGCG ACACCGGGGC 
CACCTGCGCG AGCTGGCTCA CGTGCTGCAA TCCAAGCACC GTGACAAGTA CCTGCTCTTC 
AACCTTTCAG AGAAAAGACA TGACCTGACC CGCCTAAACC CGAAGGTCCA GGACTTTGGC 
TGGCCTGAGC TGCACGCACC CCCCCTAGAC AAGCTGTGCT CCATTTGCAA AGCCATGGAG 
ACCTGGCTCA GTGCTGACCC GCAGCACGTG GTCGTATTGT ACTGCAAGGG GAGCAAGGGC 
AAGCTCGGGG TCATCGTCTC TGCCTACATG CACTACAGCA AGATCTCTGC AGGGGCAGAC 
CAGGCACTGG CGACCCTTAC CATGCGGAAG TTCTGTGAAG ACAAGGTTGC CGCAGAATTG 
CAGCCCTCTC AGCGCCGGTA TATCACCTAC TTCAGCGGTC TGCTGTCCGG GTCCATCCGC 
ATGAACAGCA GCCCTCTCTT CCTGCACCAC GTGCTGGTGC CCGTGCTGCC AGCCTTTGAA 
CCTGGTGCAG GATTCCAGCC CTTCCTCAAG ATCTACCAGT CCATGCAGCT TGTTTACACA 
TCAGGAATCT ATCACATTGC AGGCCCTGGT CCCCAGCAGC TTTGTATCAG CCTGGAGCCA 
GCCCTCCTCC TCAAAGGCGA TGTCATGGTA ACATGCTATC ACAAGGGTAG CCGGGGAACT 
GACCGGACCC TCGTATTCCG AGTCCAGTTC CACACATGCA CCATCCATGG ACCACGGCTC 
ACCTTCTCCA AGGACCAGTT GGATGAGGCC TGGACCGACG AGAGGTTCCC CTTCCAAGCC 
TCGGTGGAGT TCGTCTTCTC CTCCAGCCCA GAGAAGATCA AAGGTAACAC CCCACAGAAT 
GAGCCCTCCG TCTCTGTCGA CTACAACACT GCAGAGCCCG CAGTGCGCTG GGACTCCTAC 
GAGAACTTCA ACTTGCACCA TGAGGACAGT GCGGATGACT CTGTCACCCA CACCCGGGGG 
CCCCTGGATG GCAGTCCTTA TGCCCAGGTG CAGCGAGCCC CCCGCCAGAC CCCGCCGGCC 
CCGTCTCCGG AGCCACCCCC ACCCCCGCTG CTTTCTGTCA GCAGCGACTC TGGCCACTCC 
TCCACACTGA CCACAGAGCC AGCTGCCGAG TCCCCTGGCC GGCCACCCCC GACGGCTGCC 
GAGCGGCAGG AGCTCGAGCG CCTACTGGGA GGCTGTGGAG TGGCTAGTGG GGGCCGGGGA 
GCTGGGCGCG AGACGGCCAT CCTTGATGAC GAAGACCAGC CTGCTGCAGG CGGAGGCCCC 
CACCTCGGAA TATATTCGGG ACACAGGCCT GGCCTCAGCC GCCACTGCTC CTGCCGCCAG 
GGCTACCGGG AACCCTGCGG GGTCCCCAAT GGAGGTTACT ACCGGCCAGA GGGGACCCTG 
GAGAGGCGGC GGCTGGCTTT CGGAGCCTAT GAGGGGCCCC CCCAGGGTTA TGCTGAGCCC 
TCCGTGGAGA AGAGACGCCT CTGCCGCTCG CTGTCCGAGG GGCCGTACCC CTACCCGTCT 
GAGCTGGGGA AACCCGCCAA TGGAGACTTT GGCTACCGCC CCCCAGGCTA CCGGGAGGTG 
GTGATCCTGG AAGACCCGGG GCTGCCTCCG CTGTGCTCGT GCCCCGCCTG TGAGGAGAAG 
CTAGCACTGC CCACGGCAGC CCTCTATGGG CTGCGGCTGG AGAGGGAGGC TGGAGAGGGG 
TGGGCAAGTG AGGCTGGCAA GCCCCTCCTG CACCCAGTGC GACCCGGGCA CCCGCTGCCC 
CTGCTGGTGC CTGCCTGTGG GCACCACCAC ACCCCGCTGC CTGACTACAG CTGCTTGAAG 
CCACCCAAGG CAGGCGAGGA AGGGCATGAG GGCTGCTCCT ACGCCTTGTG CCCCGAGGGA 
AGGTATGGGC ACCCAGGGTA CCCTGCCCTG GTGACGTACG GCTATGGAGG AGCAGTTCCC 
AGTTACTGCC CAGCATATGG CCGGGTGCCG CACAGCTGTG GGTCTCCAGG TGAGGGCAGA 
GGGTATCCCA GCCCTCGTGC CCACTCCCCC CGGGCTGGCT CCATTTCCCC GGGCAGCCCG 
CCCTACCCCC AGTCCAGGAA GCTGAGCTAC GAGATCCCTG CAGAGGAGGG AGGGGACCGG 
TATCTGCTGC CTGGGCACCT GGCCCCAGCA GGACCCTTGG CATCTGCAGC TCCTCTCCCT 
GCAGAGTCGC CAGAGCCTGT GTCCTGGAGG GAGGGTCCCA GCGGGCACAG CACCCTGCCC 
CGGTCCCCGC GAGATGCCCA GTGCAGCGCT GCTTCCGAGC TGTCTGGTCC TTCCACGCCG 
CTGCACACCA GCAGCCCCGT CCAGGGCAAG GAGAGCGCCC GACGGCAGGA CACCCGCTCC 
CCCACCTTGG CGCCCACTCA GAGACTGAGT CCTGCGGAGG CCTTGCCACC TGTTTCCCAG 
GGAGGCGCTG ATAAGGCTCC AGAGCTGCCT GCAAGAAGTG GGCCTGAGCC TCCAGCCCCT 
GGCGCCTTCT CCCTAGCCTC CCCACCCAGC TCTCCCAACG ACTGGCCTCA GGAGAGGAGC 
CCGGGGGGCC GCTCAGACAG CGCCAGTCCA AGGGGGCCTG TACCCAACAC CCTGCCCGGC 
CTCCGTCATG CCCCCTGGCA GGGCCTGCGA GACCCCCCGG ACAGCCCAGA TGGGTCCCCC 
CTCACCCCTG TGCCTACTCA GATGCCCTGG CTTGTGGCCA GCCCAGAACC CCCTCAGAGC 
TCGCCCACAC CTGCCTTTCC TCTGGCTGCA TCTTACGACA TCAACGGCGC CCCCCAGCCC 
CCTCTTCCTG AGAAACGCCA CCTGCTGGGG CCTGGGCAGC AGCCGGGACC CTGGGGCCCA 
GAGCAGGCGT CCCCACCAGC CAGAGGCGCT AGTCACCATG TCACTTTTGC ACCTCTGCTC 
CCGGATAACA CCCCCCAGCC CCCAGAGCCC CCGATGCAAG AGAGCCAGAG CAATGTCAAG 
TTTGTCCAGG ACACATCCAA GTTCTGGTAC AAGCCGCACC TGTCCCGTGA CCAAGCCATC 
ACCCTGCTGA AGGACAAAGA CCCTGGGGCC TTCCTGATCA GGGACAGTCA TTCATTCCAA 
GGAGCCTATG GGCTGGCTCT CAAGGTGGCC ACGCCCCCTC CCAGCGCCCA GTCCTGGAAA 
GGGGACCCCT CAGAACAGCT GGTCCGTCAT TTTCTCATTG AGACTGGGCC GAAAGGGGTG 
AAGATCAAGG GCTGTCCCAG CGAGCCCTAC TTTGGCAGCC TGTCAGCCCT GGTCTCCCAG 
CACTCCATCT CCCCACTGTC CCTGCCCTGC TGCCTGCGCA TTCCCAGCAA AGATCCTCTG 
GAGGAGGTCC CAGAGGCCCC AGTGCCCAGC AACATGAGTA CAGCAGCAGA CCTCCTGCGT 
CAAGGCGCCG CCTGCAGCGT GCTCTACCTG ACCTCAGTGG AGACGGAGTC ACTGACGGGC 
CCCCAAGCAG TGGCACGGGC CAGCTCCGCA GCTCTGAGCT GCAGCCCCCG CCCCACACCA 
GCCGTTGTCC ACTTCAAGGT CTCAGCCCAG GGCATCACAC TCACAGACAA CCAAAGGAAG 
CTCTTCTTTC GCCGCCATTA TCCAGTCAAC AGCATCACCT TTTCCAGCAC TGACCCTCAG 
GACCGGAGAT GGACCAACTC CGACGGGACC ACCTCCAAGA TCTTTGGTTT TGTGGCCAAG 
AAGCCGGGAA GCCCTTGGGA GAACGTGTGT CACCTCTTTG CAGAGCTTGA CCCAGATCAG 
CCCGCAGGCG CCATTGTCAC CTTCATCACC AAAGTTTTAC TGGGCCAGAG GAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_015470963.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015326449.1 Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa89857
Clone ID Related Accession (Same CDS sequence)	XM_015470963.1 , XM_015463276.1
Accession Version	XM_015470963.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103919.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GAGTCGCCAG AGCCTGTGTC CTGGAGGGAG GGTCCCAGCG GGCACAGCAC CCTGCCCCGG 
TCCCCGCGAG ATGCCCAGTG CAGCGCTGCT TCCGAGCTGT CTGGTCCTTC CACGCCGCTG 
CACACCAGCA GCCCCGTCCA GGGCAAGGAG AGCGCCCGAC GGCAGGACAC CCGCTCCCCC 
ACCTTGGCGC CCACTCAGAG ACTGAGTCCT GCGGAGGCCT TGCCACCTGT TTCCCAGGGA 
GGCGCTGATA AGGCTCCAGA GCTGCCTGCA AGAAGTGGGC CTGAGCCTCC AGCCCCTGGC 
GCCTTCTCCC TAGCCTCCCC ACCCAGCTCT CCCAACGACT GGCCTCAGGA GAGGAGCCCG 
GGGGGCCGCT CAGACAGCGC CAGTCCAAGG GGGCCTGTAC CCAACACCCT GCCCGGCCTC 
CGTCATGCCC CCTGGCAGGG CCTGCGAGAC CCCCCGGACA GCCCAGATGG GTCCCCCCTC 
ACCCCTGTGC CTACTCAGAT GCCCTGGCTT GTGGCCAGCC CAGAACCCCC TCAGAGCTCG 
CCCACACCTG CCTTTCCTCT GGCTGCATCT TACGACATCA ACGGCGCCCC CCAGCCCCCT 
CTTCCTGAGA AACGCCACCT GCTGGGGCCT GGGCAGCAGC CGGGACCCTG GGGCCCAGAG 
CAGGCGTCCC CACCAGCCAG AGGCGCTAGT CACCATGTCA CTTTTGCACC TCTGCTCCCG 
GATAACACCC CCCAGCCCCC AGAGCCCCCG ATGCAAGAGA GCCAGAGCAA TGTCAAGTTT 
GTCCAGGACA CATCCAAGTT CTGGTACAAG CCGCACCTGT CCCGTGACCA AGCCATCACC 
CTGCTGAAGG ACAAAGACCC TGGGGCCTTC CTGATCAGGG ACAGTCATTC ATTCCAAGGA 
GCCTATGGGC TGGCTCTCAA GGTGGCCACG CCCCCTCCCA GCGCCCAGTC CTGGAAAGGG 
GACCCCTCAG AACAGCTGGT CCGTCATTTT CTCATTGAGA CTGGGCCGAA AGGGGTGAAG 
ATCAAGGGCT GTCCCAGCGA GCCCTACTTT GGCAGCCTGT CAGCCCTGGT CTCCCAGCAC 
TCCATCTCCC CACTGTCCCT GCCCTGCTGC CTGCGCATTC CCAGCAAAGA TCCTCTGGAG 
GAGGTCCCAG AGGCCCCAGT GCCCAGCAAC ATGAGTACAG CAGCAGACCT CCTGCGTCAA 
GGCGCCGCCT GCAGCGTGCT CTACCTGACC TCAGTGGAGA CGGAGTCACT GACGGGCCCC 
CAAGCAGTGG CACGGGCCAG CTCCGCAGCT CTGAGCTGCA GCCCCCGCCC CACACCAGCC 
GTTGTCCACT TCAAGGTCTC AGCCCAGGGC ATCACACTCA CAGACAACCA AAGGAAGCTC 
TTCTTTCGCC GCCATTATCC AGTCAACAGC ATCACCTTTT CCAGCACTGA CCCTCAGGAC 
CGGAGATGGA CCAACTCCGA CGGGACCACC TCCAAGATCT TTGGTTTTGT GGCCAAGAAG 
CCGGGAAGCC CTTGGGAGAA CGTGTGTCAC CTCTTTGCAG AGCTTGACCC AGATCAGCCC 
GCAGGCGCCA TTGTCACCTT CATCACCAAA GTTTTACTGG GCCAGAGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015326449.1
CDS	1394..5647
Translation	MENRKSAALRPGPVLEMDPWELTPLSVCPSTRQPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_015470963.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015470963.1

ORF Insert Sequence:

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GAGTCGCCAG AGCCTGTGTC CTGGAGGGAG GGTCCCAGCG GGCACAGCAC CCTGCCCCGG 
TCCCCGCGAG ATGCCCAGTG CAGCGCTGCT TCCGAGCTGT CTGGTCCTTC CACGCCGCTG 
CACACCAGCA GCCCCGTCCA GGGCAAGGAG AGCGCCCGAC GGCAGGACAC CCGCTCCCCC 
ACCTTGGCGC CCACTCAGAG ACTGAGTCCT GCGGAGGCCT TGCCACCTGT TTCCCAGGGA 
GGCGCTGATA AGGCTCCAGA GCTGCCTGCA AGAAGTGGGC CTGAGCCTCC AGCCCCTGGC 
GCCTTCTCCC TAGCCTCCCC ACCCAGCTCT CCCAACGACT GGCCTCAGGA GAGGAGCCCG 
GGGGGCCGCT CAGACAGCGC CAGTCCAAGG GGGCCTGTAC CCAACACCCT GCCCGGCCTC 
CGTCATGCCC CCTGGCAGGG CCTGCGAGAC CCCCCGGACA GCCCAGATGG GTCCCCCCTC 
ACCCCTGTGC CTACTCAGAT GCCCTGGCTT GTGGCCAGCC CAGAACCCCC TCAGAGCTCG 
CCCACACCTG CCTTTCCTCT GGCTGCATCT TACGACATCA ACGGCGCCCC CCAGCCCCCT 
CTTCCTGAGA AACGCCACCT GCTGGGGCCT GGGCAGCAGC CGGGACCCTG GGGCCCAGAG 
CAGGCGTCCC CACCAGCCAG AGGCGCTAGT CACCATGTCA CTTTTGCACC TCTGCTCCCG 
GATAACACCC CCCAGCCCCC AGAGCCCCCG ATGCAAGAGA GCCAGAGCAA TGTCAAGTTT 
GTCCAGGACA CATCCAAGTT CTGGTACAAG CCGCACCTGT CCCGTGACCA AGCCATCACC 
CTGCTGAAGG ACAAAGACCC TGGGGCCTTC CTGATCAGGG ACAGTCATTC ATTCCAAGGA 
GCCTATGGGC TGGCTCTCAA GGTGGCCACG CCCCCTCCCA GCGCCCAGTC CTGGAAAGGG 
GACCCCTCAG AACAGCTGGT CCGTCATTTT CTCATTGAGA CTGGGCCGAA AGGGGTGAAG 
ATCAAGGGCT GTCCCAGCGA GCCCTACTTT GGCAGCCTGT CAGCCCTGGT CTCCCAGCAC 
TCCATCTCCC CACTGTCCCT GCCCTGCTGC CTGCGCATTC CCAGCAAAGA TCCTCTGGAG 
GAGGTCCCAG AGGCCCCAGT GCCCAGCAAC ATGAGTACAG CAGCAGACCT CCTGCGTCAA 
GGCGCCGCCT GCAGCGTGCT CTACCTGACC TCAGTGGAGA CGGAGTCACT GACGGGCCCC 
CAAGCAGTGG CACGGGCCAG CTCCGCAGCT CTGAGCTGCA GCCCCCGCCC CACACCAGCC 
GTTGTCCACT TCAAGGTCTC AGCCCAGGGC ATCACACTCA CAGACAACCA AAGGAAGCTC 
TTCTTTCGCC GCCATTATCC AGTCAACAGC ATCACCTTTT CCAGCACTGA CCCTCAGGAC 
CGGAGATGGA CCAACTCCGA CGGGACCACC TCCAAGATCT TTGGTTTTGT GGCCAAGAAG 
CCGGGAAGCC CTTGGGAGAA CGTGTGTCAC CTCTTTGCAG AGCTTGACCC AGATCAGCCC 
GCAGGCGCCA TTGTCACCTT CATCACCAAA GTTTTACTGG GCCAGAGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_015463276.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_015318762.1 Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.

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CloneID	OBa89857
Clone ID Related Accession (Same CDS sequence)	XM_015470963.1 , XM_015463276.1
Accession Version	XM_015463276.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4254bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644788.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GAGTCGCCAG AGCCTGTGTC CTGGAGGGAG GGTCCCAGCG GGCACAGCAC CCTGCCCCGG 
TCCCCGCGAG ATGCCCAGTG CAGCGCTGCT TCCGAGCTGT CTGGTCCTTC CACGCCGCTG 
CACACCAGCA GCCCCGTCCA GGGCAAGGAG AGCGCCCGAC GGCAGGACAC CCGCTCCCCC 
ACCTTGGCGC CCACTCAGAG ACTGAGTCCT GCGGAGGCCT TGCCACCTGT TTCCCAGGGA 
GGCGCTGATA AGGCTCCAGA GCTGCCTGCA AGAAGTGGGC CTGAGCCTCC AGCCCCTGGC 
GCCTTCTCCC TAGCCTCCCC ACCCAGCTCT CCCAACGACT GGCCTCAGGA GAGGAGCCCG 
GGGGGCCGCT CAGACAGCGC CAGTCCAAGG GGGCCTGTAC CCAACACCCT GCCCGGCCTC 
CGTCATGCCC CCTGGCAGGG CCTGCGAGAC CCCCCGGACA GCCCAGATGG GTCCCCCCTC 
ACCCCTGTGC CTACTCAGAT GCCCTGGCTT GTGGCCAGCC CAGAACCCCC TCAGAGCTCG 
CCCACACCTG CCTTTCCTCT GGCTGCATCT TACGACATCA ACGGCGCCCC CCAGCCCCCT 
CTTCCTGAGA AACGCCACCT GCTGGGGCCT GGGCAGCAGC CGGGACCCTG GGGCCCAGAG 
CAGGCGTCCC CACCAGCCAG AGGCGCTAGT CACCATGTCA CTTTTGCACC TCTGCTCCCG 
GATAACACCC CCCAGCCCCC AGAGCCCCCG ATGCAAGAGA GCCAGAGCAA TGTCAAGTTT 
GTCCAGGACA CATCCAAGTT CTGGTACAAG CCGCACCTGT CCCGTGACCA AGCCATCACC 
CTGCTGAAGG ACAAAGACCC TGGGGCCTTC CTGATCAGGG ACAGTCATTC ATTCCAAGGA 
GCCTATGGGC TGGCTCTCAA GGTGGCCACG CCCCCTCCCA GCGCCCAGTC CTGGAAAGGG 
GACCCCTCAG AACAGCTGGT CCGTCATTTT CTCATTGAGA CTGGGCCGAA AGGGGTGAAG 
ATCAAGGGCT GTCCCAGCGA GCCCTACTTT GGCAGCCTGT CAGCCCTGGT CTCCCAGCAC 
TCCATCTCCC CACTGTCCCT GCCCTGCTGC CTGCGCATTC CCAGCAAAGA TCCTCTGGAG 
GAGGTCCCAG AGGCCCCAGT GCCCAGCAAC ATGAGTACAG CAGCAGACCT CCTGCGTCAA 
GGCGCCGCCT GCAGCGTGCT CTACCTGACC TCAGTGGAGA CGGAGTCACT GACGGGCCCC 
CAAGCAGTGG CACGGGCCAG CTCCGCAGCT CTGAGCTGCA GCCCCCGCCC CACACCAGCC 
GTTGTCCACT TCAAGGTCTC AGCCCAGGGC ATCACACTCA CAGACAACCA AAGGAAGCTC 
TTCTTTCGCC GCCATTATCC AGTCAACAGC ATCACCTTTT CCAGCACTGA CCCTCAGGAC 
CGGAGATGGA CCAACTCCGA CGGGACCACC TCCAAGATCT TTGGTTTTGT GGCCAAGAAG 
CCGGGAAGCC CTTGGGAGAA CGTGTGTCAC CTCTTTGCAG AGCTTGACCC AGATCAGCCC 
GCAGGCGCCA TTGTCACCTT CATCACCAAA GTTTTACTGG GCCAGAGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015318762.1
CDS	1394..5647
Translation	MENRKSAALRPGPVLEMDPWELTPLSVCPSTRQPRKAEPHSFREKVFRKKPPVCAVCKAAIDGTGVSCRVCKVATHRKCEAKVTSSCQALPPVELRRNTAPVRRIEHLGSTKSLNHSKQRSTLPRSFSLDPLMERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_015463276.1
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015463276.1

ORF Insert Sequence:

ATGGAAAACA GGAAAAGTGC AGCACTCAGG CCCGGCCCCG TCCTGGAGAT GGACCCGTGG 
GAACTGACAC CTCTGTCTGT CTGTCCCTCC ACCCGTCAGC CTAGGAAAGC TGAGCCGCAT 
AGCTTCCGAG AGAAGGTCTT CCGGAAGAAA CCACCCGTGT GTGCCGTGTG TAAGGCAGCC 
ATTGATGGGA CGGGCGTCTC ATGCAGAGTC TGCAAGGTGG CAACACACAG AAAATGTGAA 
GCAAAGGTGA CTTCGTCCTG TCAGGCTTTG CCTCCCGTGG AGCTGCGGAG AAACACCGCC 
CCTGTGAGGC GCATAGAGCA CCTGGGATCC ACCAAGTCTC TGAACCACTC AAAGCAGCGC 
AGCACTCTGC CCAGGAGCTT CAGCCTGGAC CCGCTCATGG AGCGCCGCTG GGACTTGGAC 
CTCACCTACG TGACGGAGCG GATCCTTGCC GCCTCTTTCC CCGCGCGGCC TGACGAGCAG 
CGACACCGGG GCCACCTGCG CGAGCTGGCT CACGTGCTGC AATCCAAGCA CCGTGACAAG 
TACCTGCTCT TCAACCTTTC AGAGAAAAGA CATGACCTGA CCCGCCTAAA CCCGAAGGTC 
CAGGACTTTG GCTGGCCTGA GCTGCACGCA CCCCCCCTAG ACAAGCTGTG CTCCATTTGC 
AAAGCCATGG AGACCTGGCT CAGTGCTGAC CCGCAGCACG TGGTCGTATT GTACTGCAAG 
GGGAGCAAGG GCAAGCTCGG GGTCATCGTC TCTGCCTACA TGCACTACAG CAAGATCTCT 
GCAGGGGCAG ACCAGGCACT GGCGACCCTT ACCATGCGGA AGTTCTGTGA AGACAAGGTT 
GCCGCAGAAT TGCAGCCCTC TCAGCGCCGG TATATCACCT ACTTCAGCGG TCTGCTGTCC 
GGGTCCATCC GCATGAACAG CAGCCCTCTC TTCCTGCACC ACGTGCTGGT GCCCGTGCTG 
CCAGCCTTTG AACCTGGTGC AGGATTCCAG CCCTTCCTCA AGATCTACCA GTCCATGCAG 
CTTGTTTACA CATCAGGAAT CTATCACATT GCAGGCCCTG GTCCCCAGCA GCTTTGTATC 
AGCCTGGAGC CAGCCCTCCT CCTCAAAGGC GATGTCATGG TAACATGCTA TCACAAGGGT 
AGCCGGGGAA CTGACCGGAC CCTCGTATTC CGAGTCCAGT TCCACACATG CACCATCCAT 
GGACCACGGC TCACCTTCTC CAAGGACCAG TTGGATGAGG CCTGGACCGA CGAGAGGTTC 
CCCTTCCAAG CCTCGGTGGA GTTCGTCTTC TCCTCCAGCC CAGAGAAGAT CAAAGGTAAC 
ACCCCACAGA ATGAGCCCTC CGTCTCTGTC GACTACAACA CTGCAGAGCC CGCAGTGCGC 
TGGGACTCCT ACGAGAACTT CAACTTGCAC CATGAGGACA GTGCGGATGA CTCTGTCACC 
CACACCCGGG GGCCCCTGGA TGGCAGTCCT TATGCCCAGG TGCAGCGAGC CCCCCGCCAG 
ACCCCGCCGG CCCCGTCTCC GGAGCCACCC CCACCCCCGC TGCTTTCTGT CAGCAGCGAC 
TCTGGCCACT CCTCCACACT GACCACAGAG CCAGCTGCCG AGTCCCCTGG CCGGCCACCC 
CCGACGGCTG CCGAGCGGCA GGAGCTCGAG CGCCTACTGG GAGGCTGTGG AGTGGCTAGT 
GGGGGCCGGG GAGCTGGGCG CGAGACGGCC ATCCTTGATG ACGAAGACCA GCCTGCTGCA 
GGCGGAGGCC CCCACCTCGG AATATATTCG GGACACAGGC CTGGCCTCAG CCGCCACTGC 
TCCTGCCGCC AGGGCTACCG GGAACCCTGC GGGGTCCCCA ATGGAGGTTA CTACCGGCCA 
GAGGGGACCC TGGAGAGGCG GCGGCTGGCT TTCGGAGCCT ATGAGGGGCC CCCCCAGGGT 
TATGCTGAGC CCTCCGTGGA GAAGAGACGC CTCTGCCGCT CGCTGTCCGA GGGGCCGTAC 
CCCTACCCGT CTGAGCTGGG GAAACCCGCC AATGGAGACT TTGGCTACCG CCCCCCAGGC 
TACCGGGAGG TGGTGATCCT GGAAGACCCG GGGCTGCCTC CGCTGTGCTC GTGCCCCGCC 
TGTGAGGAGA AGCTAGCACT GCCCACGGCA GCCCTCTATG GGCTGCGGCT GGAGAGGGAG 
GCTGGAGAGG GGTGGGCAAG TGAGGCTGGC AAGCCCCTCC TGCACCCAGT GCGACCCGGG 
CACCCGCTGC CCCTGCTGGT GCCTGCCTGT GGGCACCACC ACACCCCGCT GCCTGACTAC 
AGCTGCTTGA AGCCACCCAA GGCAGGCGAG GAAGGGCATG AGGGCTGCTC CTACGCCTTG 
TGCCCCGAGG GAAGGTATGG GCACCCAGGG TACCCTGCCC TGGTGACGTA CGGCTATGGA 
GGAGCAGTTC CCAGTTACTG CCCAGCATAT GGCCGGGTGC CGCACAGCTG TGGGTCTCCA 
GGTGAGGGCA GAGGGTATCC CAGCCCTCGT GCCCACTCCC CCCGGGCTGG CTCCATTTCC 
CCGGGCAGCC CGCCCTACCC CCAGTCCAGG AAGCTGAGCT ACGAGATCCC TGCAGAGGAG 
GGAGGGGACC GGTATCTGCT GCCTGGGCAC CTGGCCCCAG CAGGACCCTT GGCATCTGCA 
GAGTCGCCAG AGCCTGTGTC CTGGAGGGAG GGTCCCAGCG GGCACAGCAC CCTGCCCCGG 
TCCCCGCGAG ATGCCCAGTG CAGCGCTGCT TCCGAGCTGT CTGGTCCTTC CACGCCGCTG 
CACACCAGCA GCCCCGTCCA GGGCAAGGAG AGCGCCCGAC GGCAGGACAC CCGCTCCCCC 
ACCTTGGCGC CCACTCAGAG ACTGAGTCCT GCGGAGGCCT TGCCACCTGT TTCCCAGGGA 
GGCGCTGATA AGGCTCCAGA GCTGCCTGCA AGAAGTGGGC CTGAGCCTCC AGCCCCTGGC 
GCCTTCTCCC TAGCCTCCCC ACCCAGCTCT CCCAACGACT GGCCTCAGGA GAGGAGCCCG 
GGGGGCCGCT CAGACAGCGC CAGTCCAAGG GGGCCTGTAC CCAACACCCT GCCCGGCCTC 
CGTCATGCCC CCTGGCAGGG CCTGCGAGAC CCCCCGGACA GCCCAGATGG GTCCCCCCTC 
ACCCCTGTGC CTACTCAGAT GCCCTGGCTT GTGGCCAGCC CAGAACCCCC TCAGAGCTCG 
CCCACACCTG CCTTTCCTCT GGCTGCATCT TACGACATCA ACGGCGCCCC CCAGCCCCCT 
CTTCCTGAGA AACGCCACCT GCTGGGGCCT GGGCAGCAGC CGGGACCCTG GGGCCCAGAG 
CAGGCGTCCC CACCAGCCAG AGGCGCTAGT CACCATGTCA CTTTTGCACC TCTGCTCCCG 
GATAACACCC CCCAGCCCCC AGAGCCCCCG ATGCAAGAGA GCCAGAGCAA TGTCAAGTTT 
GTCCAGGACA CATCCAAGTT CTGGTACAAG CCGCACCTGT CCCGTGACCA AGCCATCACC 
CTGCTGAAGG ACAAAGACCC TGGGGCCTTC CTGATCAGGG ACAGTCATTC ATTCCAAGGA 
GCCTATGGGC TGGCTCTCAA GGTGGCCACG CCCCCTCCCA GCGCCCAGTC CTGGAAAGGG 
GACCCCTCAG AACAGCTGGT CCGTCATTTT CTCATTGAGA CTGGGCCGAA AGGGGTGAAG 
ATCAAGGGCT GTCCCAGCGA GCCCTACTTT GGCAGCCTGT CAGCCCTGGT CTCCCAGCAC 
TCCATCTCCC CACTGTCCCT GCCCTGCTGC CTGCGCATTC CCAGCAAAGA TCCTCTGGAG 
GAGGTCCCAG AGGCCCCAGT GCCCAGCAAC ATGAGTACAG CAGCAGACCT CCTGCGTCAA 
GGCGCCGCCT GCAGCGTGCT CTACCTGACC TCAGTGGAGA CGGAGTCACT GACGGGCCCC 
CAAGCAGTGG CACGGGCCAG CTCCGCAGCT CTGAGCTGCA GCCCCCGCCC CACACCAGCC 
GTTGTCCACT TCAAGGTCTC AGCCCAGGGC ATCACACTCA CAGACAACCA AAGGAAGCTC 
TTCTTTCGCC GCCATTATCC AGTCAACAGC ATCACCTTTT CCAGCACTGA CCCTCAGGAC 
CGGAGATGGA CCAACTCCGA CGGGACCACC TCCAAGATCT TTGGTTTTGT GGCCAAGAAG 
CCGGGAAGCC CTTGGGAGAA CGTGTGTCAC CTCTTTGCAG AGCTTGACCC AGATCAGCCC 
GCAGGCGCCA TTGTCACCTT CATCACCAAA GTTTTACTGG GCCAGAGGAA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005206246.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206303.3 Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa89858
Clone ID Related Accession (Same CDS sequence)	XM_005206246.3 , XM_005206246.4 , XM_005198240.3
Accession Version	XM_005206246.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003103919.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005206246.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGCTCCT CTCCCTGCAG AGTCGCCAGA GCCTGTGTCC 
TGGAGGGAGG GTCCCAGCGG GCACAGCACC CTGCCCCGGT CCCCGCGAGA TGCCCAGTGC 
AGCGCTGCTT CCGAGCTGTC TGGTCCTTCC ACGCCGCTGC ACACCAGCAG CCCCGTCCAG 
GGCAAGGAGA GCGCCCGACG GCAGGACACC CGCTCCCCCA CCTTGGCGCC CACTCAGAGA 
CTGAGTCCTG CGGAGGCCTT GCCACCTGTT TCCCAGGGAG GCGCTGATAA GGCTCCAGAG 
CTGCCTGCAA GAAGTGGGCC TGAGCCTCCA GCCCCTGGCG CCTTCTCCCT AGCCTCCCCA 
CCCAGCTCTC CCAACGACTG GCCTCAGGAG AGGAGCCCGG GGGGCCGCTC AGACAGCGCC 
AGTCCAAGGG GGCCTGTACC CAACACCCTG CCCGGCCTCC GTCATGCCCC CTGGCAGGGC 
CTGCGAGACC CCCCGGACAG CCCAGATGGG TCCCCCCTCA CCCCTGTGCC TACTCAGATG 
CCCTGGCTTG TGGCCAGCCC AGAACCCCCT CAGAGCTCGC CCACACCTGC CTTTCCTCTG 
GCTGCATCTT ACGACATCAA CGGCGCCCCC CAGCCCCCTC TTCCTGAGAA ACGCCACCTG 
CTGGGGCCTG GGCAGCAGCC GGGACCCTGG GGCCCAGAGC AGGCGTCCCC ACCAGCCAGA 
GGCGCTAGTC ACCATGTCAC TTTTGCACCT CTGCTCCCGG ATAACACCCC CCAGCCCCCA 
GAGCCCCCGA TGCAAGAGAG CCAGAGCAAT GTCAAGTTTG TCCAGGACAC ATCCAAGTTC 
TGGTACAAGC CGCACCTGTC CCGTGACCAA GCCATCACCC TGCTGAAGGA CAAAGACCCT 
GGGGCCTTCC TGATCAGGGA CAGTCATTCA TTCCAAGGAG CCTATGGGCT GGCTCTCAAG 
GTGGCCACGC CCCCTCCCAG CGCCCAGTCC TGGAAAGGGG ACCCCTCAGA ACAGCTGGTC 
CGTCATTTTC TCATTGAGAC TGGGCCGAAA GGGGTGAAGA TCAAGGGCTG TCCCAGCGAG 
CCCTACTTTG GCAGCCTGTC AGCCCTGGTC TCCCAGCACT CCATCTCCCC ACTGTCCCTG 
CCCTGCTGCC TGCGCATTCC CAGCAAAGAT CCTCTGGAGG AGGTCCCAGA GGCCCCAGTG 
CCCAGCAACA TGAGTACAGC AGCAGACCTC CTGCGTCAAG GCGCCGCCTG CAGCGTGCTC 
TACCTGACCT CAGTGGAGAC GGAGTCACTG ACGGGCCCCC AAGCAGTGGC ACGGGCCAGC 
TCCGCAGCTC TGAGCTGCAG CCCCCGCCCC ACACCAGCCG TTGTCCACTT CAAGGTCTCA 
GCCCAGGGCA TCACACTCAC AGACAACCAA AGGAAGCTCT TCTTTCGCCG CCATTATCCA 
GTCAACAGCA TCACCTTTTC CAGCACTGAC CCTCAGGACC GGAGATGGAC CAACTCCGAC 
GGGACCACCT CCAAGATCTT TGGTTTTGTG GCCAAGAAGC CGGGAAGCCC TTGGGAGAAC 
GTGTGTCACC TCTTTGCAGA GCTTGACCCA GATCAGCCCG CAGGCGCCAT TGTCACCTTC 
ATCACCAAAG TTTTACTGGG CCAGAGGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206303.3
CDS	370..4242
Translation	MERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005206246.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206246.3

ORF Insert Sequence:

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGCTCCT CTCCCTGCAG AGTCGCCAGA GCCTGTGTCC 
TGGAGGGAGG GTCCCAGCGG GCACAGCACC CTGCCCCGGT CCCCGCGAGA TGCCCAGTGC 
AGCGCTGCTT CCGAGCTGTC TGGTCCTTCC ACGCCGCTGC ACACCAGCAG CCCCGTCCAG 
GGCAAGGAGA GCGCCCGACG GCAGGACACC CGCTCCCCCA CCTTGGCGCC CACTCAGAGA 
CTGAGTCCTG CGGAGGCCTT GCCACCTGTT TCCCAGGGAG GCGCTGATAA GGCTCCAGAG 
CTGCCTGCAA GAAGTGGGCC TGAGCCTCCA GCCCCTGGCG CCTTCTCCCT AGCCTCCCCA 
CCCAGCTCTC CCAACGACTG GCCTCAGGAG AGGAGCCCGG GGGGCCGCTC AGACAGCGCC 
AGTCCAAGGG GGCCTGTACC CAACACCCTG CCCGGCCTCC GTCATGCCCC CTGGCAGGGC 
CTGCGAGACC CCCCGGACAG CCCAGATGGG TCCCCCCTCA CCCCTGTGCC TACTCAGATG 
CCCTGGCTTG TGGCCAGCCC AGAACCCCCT CAGAGCTCGC CCACACCTGC CTTTCCTCTG 
GCTGCATCTT ACGACATCAA CGGCGCCCCC CAGCCCCCTC TTCCTGAGAA ACGCCACCTG 
CTGGGGCCTG GGCAGCAGCC GGGACCCTGG GGCCCAGAGC AGGCGTCCCC ACCAGCCAGA 
GGCGCTAGTC ACCATGTCAC TTTTGCACCT CTGCTCCCGG ATAACACCCC CCAGCCCCCA 
GAGCCCCCGA TGCAAGAGAG CCAGAGCAAT GTCAAGTTTG TCCAGGACAC ATCCAAGTTC 
TGGTACAAGC CGCACCTGTC CCGTGACCAA GCCATCACCC TGCTGAAGGA CAAAGACCCT 
GGGGCCTTCC TGATCAGGGA CAGTCATTCA TTCCAAGGAG CCTATGGGCT GGCTCTCAAG 
GTGGCCACGC CCCCTCCCAG CGCCCAGTCC TGGAAAGGGG ACCCCTCAGA ACAGCTGGTC 
CGTCATTTTC TCATTGAGAC TGGGCCGAAA GGGGTGAAGA TCAAGGGCTG TCCCAGCGAG 
CCCTACTTTG GCAGCCTGTC AGCCCTGGTC TCCCAGCACT CCATCTCCCC ACTGTCCCTG 
CCCTGCTGCC TGCGCATTCC CAGCAAAGAT CCTCTGGAGG AGGTCCCAGA GGCCCCAGTG 
CCCAGCAACA TGAGTACAGC AGCAGACCTC CTGCGTCAAG GCGCCGCCTG CAGCGTGCTC 
TACCTGACCT CAGTGGAGAC GGAGTCACTG ACGGGCCCCC AAGCAGTGGC ACGGGCCAGC 
TCCGCAGCTC TGAGCTGCAG CCCCCGCCCC ACACCAGCCG TTGTCCACTT CAAGGTCTCA 
GCCCAGGGCA TCACACTCAC AGACAACCAA AGGAAGCTCT TCTTTCGCCG CCATTATCCA 
GTCAACAGCA TCACCTTTTC CAGCACTGAC CCTCAGGACC GGAGATGGAC CAACTCCGAC 
GGGACCACCT CCAAGATCTT TGGTTTTGTG GCCAAGAAGC CGGGAAGCCC TTGGGAGAAC 
GTGTGTCACC TCTTTGCAGA GCTTGACCCA GATCAGCCCG CAGGCGCCAT TGTCACCTTC 
ATCACCAAAG TTTTACTGGG CCAGAGGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005206246.4 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005206303.3 Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa89858
Clone ID Related Accession (Same CDS sequence)	XM_005206246.3 , XM_005206246.4 , XM_005198240.3
Accession Version	XM_005206246.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037332.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On May 7, 2018 this sequence version replaced XM_005206246.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGCTCCT CTCCCTGCAG AGTCGCCAGA GCCTGTGTCC 
TGGAGGGAGG GTCCCAGCGG GCACAGCACC CTGCCCCGGT CCCCGCGAGA TGCCCAGTGC 
AGCGCTGCTT CCGAGCTGTC TGGTCCTTCC ACGCCGCTGC ACACCAGCAG CCCCGTCCAG 
GGCAAGGAGA GCGCCCGACG GCAGGACACC CGCTCCCCCA CCTTGGCGCC CACTCAGAGA 
CTGAGTCCTG CGGAGGCCTT GCCACCTGTT TCCCAGGGAG GCGCTGATAA GGCTCCAGAG 
CTGCCTGCAA GAAGTGGGCC TGAGCCTCCA GCCCCTGGCG CCTTCTCCCT AGCCTCCCCA 
CCCAGCTCTC CCAACGACTG GCCTCAGGAG AGGAGCCCGG GGGGCCGCTC AGACAGCGCC 
AGTCCAAGGG GGCCTGTACC CAACACCCTG CCCGGCCTCC GTCATGCCCC CTGGCAGGGC 
CTGCGAGACC CCCCGGACAG CCCAGATGGG TCCCCCCTCA CCCCTGTGCC TACTCAGATG 
CCCTGGCTTG TGGCCAGCCC AGAACCCCCT CAGAGCTCGC CCACACCTGC CTTTCCTCTG 
GCTGCATCTT ACGACATCAA CGGCGCCCCC CAGCCCCCTC TTCCTGAGAA ACGCCACCTG 
CTGGGGCCTG GGCAGCAGCC GGGACCCTGG GGCCCAGAGC AGGCGTCCCC ACCAGCCAGA 
GGCGCTAGTC ACCATGTCAC TTTTGCACCT CTGCTCCCGG ATAACACCCC CCAGCCCCCA 
GAGCCCCCGA TGCAAGAGAG CCAGAGCAAT GTCAAGTTTG TCCAGGACAC ATCCAAGTTC 
TGGTACAAGC CGCACCTGTC CCGTGACCAA GCCATCACCC TGCTGAAGGA CAAAGACCCT 
GGGGCCTTCC TGATCAGGGA CAGTCATTCA TTCCAAGGAG CCTATGGGCT GGCTCTCAAG 
GTGGCCACGC CCCCTCCCAG CGCCCAGTCC TGGAAAGGGG ACCCCTCAGA ACAGCTGGTC 
CGTCATTTTC TCATTGAGAC TGGGCCGAAA GGGGTGAAGA TCAAGGGCTG TCCCAGCGAG 
CCCTACTTTG GCAGCCTGTC AGCCCTGGTC TCCCAGCACT CCATCTCCCC ACTGTCCCTG 
CCCTGCTGCC TGCGCATTCC CAGCAAAGAT CCTCTGGAGG AGGTCCCAGA GGCCCCAGTG 
CCCAGCAACA TGAGTACAGC AGCAGACCTC CTGCGTCAAG GCGCCGCCTG CAGCGTGCTC 
TACCTGACCT CAGTGGAGAC GGAGTCACTG ACGGGCCCCC AAGCAGTGGC ACGGGCCAGC 
TCCGCAGCTC TGAGCTGCAG CCCCCGCCCC ACACCAGCCG TTGTCCACTT CAAGGTCTCA 
GCCCAGGGCA TCACACTCAC AGACAACCAA AGGAAGCTCT TCTTTCGCCG CCATTATCCA 
GTCAACAGCA TCACCTTTTC CAGCACTGAC CCTCAGGACC GGAGATGGAC CAACTCCGAC 
GGGACCACCT CCAAGATCTT TGGTTTTGTG GCCAAGAAGC CGGGAAGCCC TTGGGAGAAC 
GTGTGTCACC TCTTTGCAGA GCTTGACCCA GATCAGCCCG CAGGCGCCAT TGTCACCTTC 
ATCACCAAAG TTTTACTGGG CCAGAGGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005206303.3
CDS	367..4239
Translation	MERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005206246.4
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005206246.4

ORF Insert Sequence:

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGCTCCT CTCCCTGCAG AGTCGCCAGA GCCTGTGTCC 
TGGAGGGAGG GTCCCAGCGG GCACAGCACC CTGCCCCGGT CCCCGCGAGA TGCCCAGTGC 
AGCGCTGCTT CCGAGCTGTC TGGTCCTTCC ACGCCGCTGC ACACCAGCAG CCCCGTCCAG 
GGCAAGGAGA GCGCCCGACG GCAGGACACC CGCTCCCCCA CCTTGGCGCC CACTCAGAGA 
CTGAGTCCTG CGGAGGCCTT GCCACCTGTT TCCCAGGGAG GCGCTGATAA GGCTCCAGAG 
CTGCCTGCAA GAAGTGGGCC TGAGCCTCCA GCCCCTGGCG CCTTCTCCCT AGCCTCCCCA 
CCCAGCTCTC CCAACGACTG GCCTCAGGAG AGGAGCCCGG GGGGCCGCTC AGACAGCGCC 
AGTCCAAGGG GGCCTGTACC CAACACCCTG CCCGGCCTCC GTCATGCCCC CTGGCAGGGC 
CTGCGAGACC CCCCGGACAG CCCAGATGGG TCCCCCCTCA CCCCTGTGCC TACTCAGATG 
CCCTGGCTTG TGGCCAGCCC AGAACCCCCT CAGAGCTCGC CCACACCTGC CTTTCCTCTG 
GCTGCATCTT ACGACATCAA CGGCGCCCCC CAGCCCCCTC TTCCTGAGAA ACGCCACCTG 
CTGGGGCCTG GGCAGCAGCC GGGACCCTGG GGCCCAGAGC AGGCGTCCCC ACCAGCCAGA 
GGCGCTAGTC ACCATGTCAC TTTTGCACCT CTGCTCCCGG ATAACACCCC CCAGCCCCCA 
GAGCCCCCGA TGCAAGAGAG CCAGAGCAAT GTCAAGTTTG TCCAGGACAC ATCCAAGTTC 
TGGTACAAGC CGCACCTGTC CCGTGACCAA GCCATCACCC TGCTGAAGGA CAAAGACCCT 
GGGGCCTTCC TGATCAGGGA CAGTCATTCA TTCCAAGGAG CCTATGGGCT GGCTCTCAAG 
GTGGCCACGC CCCCTCCCAG CGCCCAGTCC TGGAAAGGGG ACCCCTCAGA ACAGCTGGTC 
CGTCATTTTC TCATTGAGAC TGGGCCGAAA GGGGTGAAGA TCAAGGGCTG TCCCAGCGAG 
CCCTACTTTG GCAGCCTGTC AGCCCTGGTC TCCCAGCACT CCATCTCCCC ACTGTCCCTG 
CCCTGCTGCC TGCGCATTCC CAGCAAAGAT CCTCTGGAGG AGGTCCCAGA GGCCCCAGTG 
CCCAGCAACA TGAGTACAGC AGCAGACCTC CTGCGTCAAG GCGCCGCCTG CAGCGTGCTC 
TACCTGACCT CAGTGGAGAC GGAGTCACTG ACGGGCCCCC AAGCAGTGGC ACGGGCCAGC 
TCCGCAGCTC TGAGCTGCAG CCCCCGCCCC ACACCAGCCG TTGTCCACTT CAAGGTCTCA 
GCCCAGGGCA TCACACTCAC AGACAACCAA AGGAAGCTCT TCTTTCGCCG CCATTATCCA 
GTCAACAGCA TCACCTTTTC CAGCACTGAC CCTCAGGACC GGAGATGGAC CAACTCCGAC 
GGGACCACCT CCAAGATCTT TGGTTTTGTG GCCAAGAAGC CGGGAAGCCC TTGGGAGAAC 
GTGTGTCACC TCTTTGCAGA GCTTGACCCA GATCAGCCCG CAGGCGCCAT TGTCACCTTC 
ATCACCAAAG TTTTACTGGG CCAGAGGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

TNS2 ( XM_005198240.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005198297.3 Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.

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CloneID	OBa89858
Clone ID Related Accession (Same CDS sequence)	XM_005206246.3 , XM_005206246.4 , XM_005198240.3
Accession Version	XM_005198240.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	tensin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_014644788.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005198240.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGCTCCT CTCCCTGCAG AGTCGCCAGA GCCTGTGTCC 
TGGAGGGAGG GTCCCAGCGG GCACAGCACC CTGCCCCGGT CCCCGCGAGA TGCCCAGTGC 
AGCGCTGCTT CCGAGCTGTC TGGTCCTTCC ACGCCGCTGC ACACCAGCAG CCCCGTCCAG 
GGCAAGGAGA GCGCCCGACG GCAGGACACC CGCTCCCCCA CCTTGGCGCC CACTCAGAGA 
CTGAGTCCTG CGGAGGCCTT GCCACCTGTT TCCCAGGGAG GCGCTGATAA GGCTCCAGAG 
CTGCCTGCAA GAAGTGGGCC TGAGCCTCCA GCCCCTGGCG CCTTCTCCCT AGCCTCCCCA 
CCCAGCTCTC CCAACGACTG GCCTCAGGAG AGGAGCCCGG GGGGCCGCTC AGACAGCGCC 
AGTCCAAGGG GGCCTGTACC CAACACCCTG CCCGGCCTCC GTCATGCCCC CTGGCAGGGC 
CTGCGAGACC CCCCGGACAG CCCAGATGGG TCCCCCCTCA CCCCTGTGCC TACTCAGATG 
CCCTGGCTTG TGGCCAGCCC AGAACCCCCT CAGAGCTCGC CCACACCTGC CTTTCCTCTG 
GCTGCATCTT ACGACATCAA CGGCGCCCCC CAGCCCCCTC TTCCTGAGAA ACGCCACCTG 
CTGGGGCCTG GGCAGCAGCC GGGACCCTGG GGCCCAGAGC AGGCGTCCCC ACCAGCCAGA 
GGCGCTAGTC ACCATGTCAC TTTTGCACCT CTGCTCCCGG ATAACACCCC CCAGCCCCCA 
GAGCCCCCGA TGCAAGAGAG CCAGAGCAAT GTCAAGTTTG TCCAGGACAC ATCCAAGTTC 
TGGTACAAGC CGCACCTGTC CCGTGACCAA GCCATCACCC TGCTGAAGGA CAAAGACCCT 
GGGGCCTTCC TGATCAGGGA CAGTCATTCA TTCCAAGGAG CCTATGGGCT GGCTCTCAAG 
GTGGCCACGC CCCCTCCCAG CGCCCAGTCC TGGAAAGGGG ACCCCTCAGA ACAGCTGGTC 
CGTCATTTTC TCATTGAGAC TGGGCCGAAA GGGGTGAAGA TCAAGGGCTG TCCCAGCGAG 
CCCTACTTTG GCAGCCTGTC AGCCCTGGTC TCCCAGCACT CCATCTCCCC ACTGTCCCTG 
CCCTGCTGCC TGCGCATTCC CAGCAAAGAT CCTCTGGAGG AGGTCCCAGA GGCCCCAGTG 
CCCAGCAACA TGAGTACAGC AGCAGACCTC CTGCGTCAAG GCGCCGCCTG CAGCGTGCTC 
TACCTGACCT CAGTGGAGAC GGAGTCACTG ACGGGCCCCC AAGCAGTGGC ACGGGCCAGC 
TCCGCAGCTC TGAGCTGCAG CCCCCGCCCC ACACCAGCCG TTGTCCACTT CAAGGTCTCA 
GCCCAGGGCA TCACACTCAC AGACAACCAA AGGAAGCTCT TCTTTCGCCG CCATTATCCA 
GTCAACAGCA TCACCTTTTC CAGCACTGAC CCTCAGGACC GGAGATGGAC CAACTCCGAC 
GGGACCACCT CCAAGATCTT TGGTTTTGTG GCCAAGAAGC CGGGAAGCCC TTGGGAGAAC 
GTGTGTCACC TCTTTGCAGA GCTTGACCCA GATCAGCCCG CAGGCGCCAT TGTCACCTTC 
ATCACCAAAG TTTTACTGGG CCAGAGGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005198297.3
CDS	370..4242
Translation	MERRWDLDLTYVTERILAASFPARPDEQRHRGHLRELAHVLQSKHRDKYLLFNLSEKRHDLTRLNPKVQDFGWPELHAPPLDKLCSICKAMETWLSADPQHVVVLYCKGSKGKLGVIVSAYMHYSKISAGADQALATLTMRKFCEDKVAAELQPSQRRYITYFSGLLSGSIRMNSSPLFLHHVLVPVLPAFEPGAGFQPFLKIYQSMQLVYTSGIYHIAGPGPQQLCISLEPALLLKGDVMVTCYHKGSRGTDRTLVFRVQFHTCTIHGPRLTFSKDQLDEAWTDERFPFQASVEFVFSSSPEKIKGNTPQNEPSVSVDYNTAEPAVRWDSYENFNLHHEDSADDSVTHTRGPLDGSPYAQVQRAPRQTPPAPSPEPPPPPLLSVSSDSGHSSTLTTEPAAESPGRPPPTAAERQELERLLGGCGVASGGRGAGRETAILDDEDQPAAGGGPHLGIYSGHRPGLSRHCSCRQGYREPCGVPNGGYYRPEGTLERRRLAFGAYEGPPQGYAEPSVEKRRLCRSLSEGPYPYPSELGKPANGDFGYRPPGYREVVILEDPGLPPLCSCPACEEKLALPTAALYGLRLEREAGEGWASEAGKPLLHPVRPGHPLPLLVPACGHHHTPLPDYSCLKPPKAGEEGHEGCSYALCPEGRYGHPGYPALVTYGYGGAVPSYCPAYGRVPHSCGSPGEGRGYPSPRAHSPRAGSISPGSPPYPQSRKLSYEIPAEEGGDRYLLPGHLAPAGPLASAAPLPAESPEPVSWREGPSGHSTLPRSPRDAQCSAASELSGPSTPLHTSSPVQGKESARRQDTRSPTLAPTQRLSPAEALPPVSQGGADKAPELPARSGPEPPAPGAFSLASPPSSPNDWPQERSPGGRSDSASPRGPVPNTLPGLRHAPWQGLRDPPDSPDGSPLTPVPTQMPWLVASPEPPQSSPTPAFPLAASYDINGAPQPPLPEKRHLLGPGQQPGPWGPEQASPPARGASHHVTFAPLLPDNTPQPPEPPMQESQSNVKFVQDTSKFWYKPHLSRDQAITLLKDKDPGAFLIRDSHSFQGAYGLALKVATPPPSAQSWKGDPSEQLVRHFLIETGPKGVKIKGCPSEPYFGSLSALVSQHSISPLSLPCCLRIPSKDPLEEVPEAPVPSNMSTAADLLRQGAACSVLYLTSVETESLTGPQAVARASSAALSCSPRPTPAVVHFKVSAQGITLTDNQRKLFFRRHYPVNSITFSSTDPQDRRWTNSDGTTSKIFGFVAKKPGSPWENVCHLFAELDPDQPAGAIVTFITKVLLGQRK

Target ORF information:

RefSeq Version	XM_005198240.3
Organism	Bos taurus(cattle)
Definition	Bos taurus tensin 2 (TNS2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005198240.3

ORF Insert Sequence:

ATGGAGCGCC GCTGGGACTT GGACCTCACC TACGTGACGG AGCGGATCCT TGCCGCCTCT 
TTCCCCGCGC GGCCTGACGA GCAGCGACAC CGGGGCCACC TGCGCGAGCT GGCTCACGTG 
CTGCAATCCA AGCACCGTGA CAAGTACCTG CTCTTCAACC TTTCAGAGAA AAGACATGAC 
CTGACCCGCC TAAACCCGAA GGTCCAGGAC TTTGGCTGGC CTGAGCTGCA CGCACCCCCC 
CTAGACAAGC TGTGCTCCAT TTGCAAAGCC ATGGAGACCT GGCTCAGTGC TGACCCGCAG 
CACGTGGTCG TATTGTACTG CAAGGGGAGC AAGGGCAAGC TCGGGGTCAT CGTCTCTGCC 
TACATGCACT ACAGCAAGAT CTCTGCAGGG GCAGACCAGG CACTGGCGAC CCTTACCATG 
CGGAAGTTCT GTGAAGACAA GGTTGCCGCA GAATTGCAGC CCTCTCAGCG CCGGTATATC 
ACCTACTTCA GCGGTCTGCT GTCCGGGTCC ATCCGCATGA ACAGCAGCCC TCTCTTCCTG 
CACCACGTGC TGGTGCCCGT GCTGCCAGCC TTTGAACCTG GTGCAGGATT CCAGCCCTTC 
CTCAAGATCT ACCAGTCCAT GCAGCTTGTT TACACATCAG GAATCTATCA CATTGCAGGC 
CCTGGTCCCC AGCAGCTTTG TATCAGCCTG GAGCCAGCCC TCCTCCTCAA AGGCGATGTC 
ATGGTAACAT GCTATCACAA GGGTAGCCGG GGAACTGACC GGACCCTCGT ATTCCGAGTC 
CAGTTCCACA CATGCACCAT CCATGGACCA CGGCTCACCT TCTCCAAGGA CCAGTTGGAT 
GAGGCCTGGA CCGACGAGAG GTTCCCCTTC CAAGCCTCGG TGGAGTTCGT CTTCTCCTCC 
AGCCCAGAGA AGATCAAAGG TAACACCCCA CAGAATGAGC CCTCCGTCTC TGTCGACTAC 
AACACTGCAG AGCCCGCAGT GCGCTGGGAC TCCTACGAGA ACTTCAACTT GCACCATGAG 
GACAGTGCGG ATGACTCTGT CACCCACACC CGGGGGCCCC TGGATGGCAG TCCTTATGCC 
CAGGTGCAGC GAGCCCCCCG CCAGACCCCG CCGGCCCCGT CTCCGGAGCC ACCCCCACCC 
CCGCTGCTTT CTGTCAGCAG CGACTCTGGC CACTCCTCCA CACTGACCAC AGAGCCAGCT 
GCCGAGTCCC CTGGCCGGCC ACCCCCGACG GCTGCCGAGC GGCAGGAGCT CGAGCGCCTA 
CTGGGAGGCT GTGGAGTGGC TAGTGGGGGC CGGGGAGCTG GGCGCGAGAC GGCCATCCTT 
GATGACGAAG ACCAGCCTGC TGCAGGCGGA GGCCCCCACC TCGGAATATA TTCGGGACAC 
AGGCCTGGCC TCAGCCGCCA CTGCTCCTGC CGCCAGGGCT ACCGGGAACC CTGCGGGGTC 
CCCAATGGAG GTTACTACCG GCCAGAGGGG ACCCTGGAGA GGCGGCGGCT GGCTTTCGGA 
GCCTATGAGG GGCCCCCCCA GGGTTATGCT GAGCCCTCCG TGGAGAAGAG ACGCCTCTGC 
CGCTCGCTGT CCGAGGGGCC GTACCCCTAC CCGTCTGAGC TGGGGAAACC CGCCAATGGA 
GACTTTGGCT ACCGCCCCCC AGGCTACCGG GAGGTGGTGA TCCTGGAAGA CCCGGGGCTG 
CCTCCGCTGT GCTCGTGCCC CGCCTGTGAG GAGAAGCTAG CACTGCCCAC GGCAGCCCTC 
TATGGGCTGC GGCTGGAGAG GGAGGCTGGA GAGGGGTGGG CAAGTGAGGC TGGCAAGCCC 
CTCCTGCACC CAGTGCGACC CGGGCACCCG CTGCCCCTGC TGGTGCCTGC CTGTGGGCAC 
CACCACACCC CGCTGCCTGA CTACAGCTGC TTGAAGCCAC CCAAGGCAGG CGAGGAAGGG 
CATGAGGGCT GCTCCTACGC CTTGTGCCCC GAGGGAAGGT ATGGGCACCC AGGGTACCCT 
GCCCTGGTGA CGTACGGCTA TGGAGGAGCA GTTCCCAGTT ACTGCCCAGC ATATGGCCGG 
GTGCCGCACA GCTGTGGGTC TCCAGGTGAG GGCAGAGGGT ATCCCAGCCC TCGTGCCCAC 
TCCCCCCGGG CTGGCTCCAT TTCCCCGGGC AGCCCGCCCT ACCCCCAGTC CAGGAAGCTG 
AGCTACGAGA TCCCTGCAGA GGAGGGAGGG GACCGGTATC TGCTGCCTGG GCACCTGGCC 
CCAGCAGGAC CCTTGGCATC TGCAGCTCCT CTCCCTGCAG AGTCGCCAGA GCCTGTGTCC 
TGGAGGGAGG GTCCCAGCGG GCACAGCACC CTGCCCCGGT CCCCGCGAGA TGCCCAGTGC 
AGCGCTGCTT CCGAGCTGTC TGGTCCTTCC ACGCCGCTGC ACACCAGCAG CCCCGTCCAG 
GGCAAGGAGA GCGCCCGACG GCAGGACACC CGCTCCCCCA CCTTGGCGCC CACTCAGAGA 
CTGAGTCCTG CGGAGGCCTT GCCACCTGTT TCCCAGGGAG GCGCTGATAA GGCTCCAGAG 
CTGCCTGCAA GAAGTGGGCC TGAGCCTCCA GCCCCTGGCG CCTTCTCCCT AGCCTCCCCA 
CCCAGCTCTC CCAACGACTG GCCTCAGGAG AGGAGCCCGG GGGGCCGCTC AGACAGCGCC 
AGTCCAAGGG GGCCTGTACC CAACACCCTG CCCGGCCTCC GTCATGCCCC CTGGCAGGGC 
CTGCGAGACC CCCCGGACAG CCCAGATGGG TCCCCCCTCA CCCCTGTGCC TACTCAGATG 
CCCTGGCTTG TGGCCAGCCC AGAACCCCCT CAGAGCTCGC CCACACCTGC CTTTCCTCTG 
GCTGCATCTT ACGACATCAA CGGCGCCCCC CAGCCCCCTC TTCCTGAGAA ACGCCACCTG 
CTGGGGCCTG GGCAGCAGCC GGGACCCTGG GGCCCAGAGC AGGCGTCCCC ACCAGCCAGA 
GGCGCTAGTC ACCATGTCAC TTTTGCACCT CTGCTCCCGG ATAACACCCC CCAGCCCCCA 
GAGCCCCCGA TGCAAGAGAG CCAGAGCAAT GTCAAGTTTG TCCAGGACAC ATCCAAGTTC 
TGGTACAAGC CGCACCTGTC CCGTGACCAA GCCATCACCC TGCTGAAGGA CAAAGACCCT 
GGGGCCTTCC TGATCAGGGA CAGTCATTCA TTCCAAGGAG CCTATGGGCT GGCTCTCAAG 
GTGGCCACGC CCCCTCCCAG CGCCCAGTCC TGGAAAGGGG ACCCCTCAGA ACAGCTGGTC 
CGTCATTTTC TCATTGAGAC TGGGCCGAAA GGGGTGAAGA TCAAGGGCTG TCCCAGCGAG 
CCCTACTTTG GCAGCCTGTC AGCCCTGGTC TCCCAGCACT CCATCTCCCC ACTGTCCCTG 
CCCTGCTGCC TGCGCATTCC CAGCAAAGAT CCTCTGGAGG AGGTCCCAGA GGCCCCAGTG 
CCCAGCAACA TGAGTACAGC AGCAGACCTC CTGCGTCAAG GCGCCGCCTG CAGCGTGCTC 
TACCTGACCT CAGTGGAGAC GGAGTCACTG ACGGGCCCCC AAGCAGTGGC ACGGGCCAGC 
TCCGCAGCTC TGAGCTGCAG CCCCCGCCCC ACACCAGCCG TTGTCCACTT CAAGGTCTCA 
GCCCAGGGCA TCACACTCAC AGACAACCAA AGGAAGCTCT TCTTTCGCCG CCATTATCCA 
GTCAACAGCA TCACCTTTTC CAGCACTGAC CCTCAGGACC GGAGATGGAC CAACTCCGAC 
GGGACCACCT CCAAGATCTT TGGTTTTGTG GCCAAGAAGC CGGGAAGCCC TTGGGAGAAC 
GTGTGTCACC TCTTTGCAGA GCTTGACCCA GATCAGCCCG CAGGCGCCAT TGTCACCTTC 
ATCACCAAAG TTTTACTGGG CCAGAGGAAA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	TNS2
Entrez Gene ID	540153
Full Name	tensin 2
Synonyms	TENC1
General protein information	Preferred Names tensin 2 Names tensin-2 tensin like C1 domain containing phosphatase (tensin 2)
Gene Type	protein-coding
Organism	Bos taurus(cattle)

Homo sapiens (human)	TENC1	NP_736610.2
Pan troglodytes (chimpanzee)	TENC1	XP_509087.3
	TENC1	XP_001102202.1
Danio rerio (zebrafish)	tenc1b	XP_694169.6
Rattus norvegicus (Norway rat)	Tenc1	XP_003750459.2
Danio rerio (zebrafish)	tenc1a	XP_001921043.2
Xenopus tropicalis (tropical clawed frog)	tenc1	XP_002936689.2
Bos taurus (cattle)	TENC1	XP_005206300.1
Mus musculus (house mouse)	Tenc1	NP_705761.2
Canis lupus familiaris (dog)	TENC1	XP_534792.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

TNS2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

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