tns2a gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_001921008.5	XP_001921043.3	tensin-2 isoform X1
XM_021469904.1	XP_021325579.1	tensin-2 isoform X1
XM_021469905.1	XP_021325580.1	tensin-2 isoform X2
XM_009296675.2	XP_009294950.1	tensin-2 isoform X4
XM_009296677.2	XP_009294952.1	tensin-2 isoform X5
XM_005161970.3	XP_005162027.1	tensin-2 isoform X2
XM_005161971.3	XP_005162028.1	tensin-2 isoform X3
XM_005161970.4	XP_005162027.2	tensin-2 isoform X2
XM_021469907.1	XP_021325582.1	tensin-2 isoform X5
XM_009296676.2	XP_009294951.1	tensin-2 isoform X4
XM_009296677.3	XP_009294952.2	tensin-2 isoform X5
XM_001921008.4	XP_001921043.2	tensin-2 isoform X1
XM_021469906.1	XP_021325581.1	tensin-2 isoform X3
XM_009296675.3	XP_009294950.1	tensin-2 isoform X4
XM_021473564.1	XP_021329239.1	tensin-2 isoform X4
XM_009296676.3	XP_009294951.1	tensin-2 isoform X4
XM_005161971.4	XP_005162028.1	tensin-2 isoform X3

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146213	XM_001921008.5 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa146214	XM_005161970.4 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa146215	XM_021473564.1 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa146216	XM_009296677.3 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa27477	XM_021469904.1 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27477	XM_001921008.4 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27478	XM_021469905.1 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27478	XM_005161970.3 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27479	XM_021469906.1 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27479	XM_005161971.4 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27480	XM_009296675.3 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27480	XM_009296676.3 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa47336	XM_021469907.1 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa47336	XM_009296677.2 Latest version!	Danio rerio tensin 2a (tns2a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
View Old Accession Versions >>
ODa27480	XM_009296675.2	Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27480	XM_009296676.2	Danio rerio tensin 2a (tns2a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa27479	XM_005161971.3	Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

tns2a ( XM_001921008.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921043.3 Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146213
Clone ID Related Accession (Same CDS sequence)	XM_001921008.5
Accession Version	XM_001921008.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001921008.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTAAGTGG GGATAGCTGT GGGGACGAGC CAGATCCAGT ACCCGTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGG 
TGGACTAACT CAGACAGCAC GACCTCTAAA GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC 
AGTGTGGCAG AGAATGTGTG CCATCTTTTC GCAGAGCTGG ACCCTGAACA GCCCGCGACC 
GCCATCGTCA ACTTCATCAA CAAAGTGATG CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921043.3
CDS	260..5110
Translation	MGCALSGDSCGDEPDPVPVRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_001921008.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921008.5

ORF Insert Sequence:

ATGGGTTGCG CCTTAAGTGG GGATAGCTGT GGGGACGAGC CAGATCCAGT ACCCGTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGG 
TGGACTAACT CAGACAGCAC GACCTCTAAA GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC 
AGTGTGGCAG AGAATGTGTG CCATCTTTTC GCAGAGCTGG ACCCTGAACA GCCCGCGACC 
GCCATCGTCA ACTTCATCAA CAAAGTGATG CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_005161970.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162027.2 Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa146214
Clone ID Related Accession (Same CDS sequence)	XM_005161970.4
Accession Version	XM_005161970.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161970.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTAAGTGG GGATAGCTGT GGGGACGAGC CAGATCCAGT ACCCGTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGA 
GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC AGTGTGGCAG AGAATGTGTG CCATCTTTTC 
GCAGAGCTGG ACCCTGAACA GCCCGCGACC GCCATCGTCA ACTTCATCAA CAAAGTGATG 
CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162027.2
CDS	260..5080
Translation	MGCALSGDSCGDEPDPVPVRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_005161970.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161970.4

ORF Insert Sequence:

ATGGGTTGCG CCTTAAGTGG GGATAGCTGT GGGGACGAGC CAGATCCAGT ACCCGTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGA 
GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC AGTGTGGCAG AGAATGTGTG CCATCTTTTC 
GCAGAGCTGG ACCCTGAACA GCCCGCGACC GCCATCGTCA ACTTCATCAA CAAAGTGATG 
CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_021473564.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329239.1 Danio rerio tensin 2a (tns2a), transcript variant X7, mRNA.

Price & Availability↑

CloneID	ODa146215
Clone ID Related Accession (Same CDS sequence)	XM_021473564.1
Accession Version	XM_021473564.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCT GTTCATGGAA AGAAAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329239.1
CDS	12..4805
Translation	MSCCSWKERQRPEFHRTVQAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_021473564.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473564.1

ORF Insert Sequence:

ATGAGCTGCT GTTCATGGAA AGAAAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_009296677.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294952.2 Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.

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CloneID	ODa146216
Clone ID Related Accession (Same CDS sequence)	XM_009296677.3
Accession Version	XM_009296677.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296677.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTAAGTGG GGATAGCTGT GGGGACGAGC CAGATCCAGT ACCCGTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGACCTGC TCTGGGACCG CCAGCAAGGT 
CCACAGCACT ACCTGCATCG CACTTGTTCA GAGGGACCAT CTCGCCACCT GTATCCATAT 
CCATCCCAAG AGATCAATTC TCATCCACAC AGTCTGTCCC CCCGACTGCT TTGTAGACCA 
GATGAGTACA TTTCATATCC TCACCATCAT CACAATCATT CACACCATCC CAAACCCAGC 
GGACCCTACC ATGATGTCAT GATGATAGAT GGCCTAGTGA CACCCGGCTG CCCCTGTCGA 
GACTGTGCTC TACGACGAGA AGAGTTCCAC ACTTTACGTT TGGACAGAGA GGATGGAATA 
CACTGGGAGA GAGAGGCAGA GCTTCACCGG GATAATGGGT TAAGACGGAC AAGAGAGGCT 
GACATGCAGA GAGGGGCAGA GATATGGGAG CGGGAGCCAG GACTAAGGAG AGGAAGAGAG 
ATGTCTCTTC ACTGGGACAG AGACAGAGAT GCAGAACTAC AGTGGGAGAG AGAACGTGAG 
GCTGAATACT GGCACAGAAG GGCTACTGTA TCCCCATACG GGCCCCACGG TCATGAATTA 
CCTGCGTTTA ATTTTGATCC ACTCCCAGCA GGCCATCCTG CATACCCAGA ACCCTCCCGC 
TCTCACTCTC ATGCCCATCT GGATCTTAAA TACAGCAGCA GCTCCAGTGG CTACCAGACA 
CCACACCAGA CGTGCCCTTG CTCCCCCTAC CAGCCTTCAC CCTCAGAGAG TCGAGGATAT 
GCCTCGGGTT ACCAGTCTGA GTCTACGTCT CCTCTTCCGC CTCCATCTGC CTCCTGCCCC 
GGCCCGGTGC ACCATGCCAG TGGACCTACA GAAAACCAAA CTGACCCCCA TTCTCAGCAT 
TTCACCTCTG ACTCAAATAC AGATTGCCCT GGTGCAGATA GCATAGGATG GCGTAATCAT 
ATTACCCATG GATCCCTCCG GCGGATGCAT CCACATGCTC CATATTCAAC ACCCTCTGAT 
ATTTCAGGAC CTCCAACACC AGTCCACACA AGCAGCCCTT TACGAACCCA GGAAAGTCCA 
AGCCTGAGTG TCCGTGACTA TGACTCAAGA GAGGGGAACA GTGAAAGCAG TTCTGCTCAC 
TCCCTCAACA GGCCGCATCC AGCTACCATA CAGCCCAACA TGACCAATGC AGGATCACGG 
CAAACTCTAC TCTCTTCCAG TCCAGAGACT CCTGAGATTA ACCCGCTTCC TACCACACCC 
ACCACTGCCC AACATCTTGC AGCTCATTCC CTTCCTACAA CACCTCCAAC CCCACAAATT 
GCAACAGAAC ACTCACCCCC CTTAACGCCC GAGTCAGAAT CCACCCAACC ACAGGCAAAT 
TGCTCGCCTA CTGCTGCTAC AGTGCTGCCC ACAGTGGAAA TGGTGCCACA ATCAACTGAG 
ACTATAAAAC CAGCACATTC CCCACATCCC TCCTCTCCAG TCCTGGCTGA GTCCATTCCA 
GCTCACATGC AAAGCAATGG AGTACCACCA TCTCCTGAAT CAGATGCCCC TAAACCTGCT 
TCTTCAGCAC CTGCATCCCC TCAGCCACCT GCTGGTGACC ACGAGAGCTC TTCTTCCCCT 
GAACCTTCAG TGCCTGGATT TGCCACACTG GGCAGGAAGT TGATGTTGGG CTCTGAAACC 
TCACTTTCTG CAGGACACAG TATGGATGCC AGTTCTAATT CTACTCCCAC TTTCCCAGTC 
TCTCCTGCCT GCTGTACTCC ATCTCCTCCA CCAGGCTCCT ACCCAGGGTG CCCATCATCC 
TCCGTCCCAC AGCCTCCTCT CCCAGAGAAA CGCCGCCCAT CCGGCCTCCC AGGCTCACCC 
AATGGGAGGT CTGGAACACT GAGGGCATCC ATGAGTCACC AGCCCTCTGG GCAGCACCAT 
GTTACATTCT CGCCCTCAGT AGGGGAGCTG GCCGTACCCA TTGGTCAGGT TGAAGTTGGT 
GTGGAGGGTG AGATTGGCAA CCGTGTCAGT GTGAAATTTG TGCAGGACAG CTCACGATTT 
TGGTATAAAC CTGGCATCTC CAGAGAGCAA GCTATAGCAG CACTGAAGGA AAGAGAGCCA 
GGGGCCTTCC TCATCCGAGA CAGTAACTCC TTCCAGGGGG CCTACGGTCT GGCCCTGAAA 
GTTGCCAGCC CACCCCCCAA CGTCAATAAT CATAGCAGCA AAGGAGACCC TCTGGAACAG 
CTGGTTCGTC ACTTTCTGAT TGAGACGGGG CCGCGAGGGG TGAAGATCAA GGGCTGTCAG 
AACGAGCCCT ACTTTGGGAG TCTTTCAGCA CTGGTGTATC AGCATTCCAT TACACCCATC 
TCTCTGCCCT GTGCCCTCCG TATCCCAGAG AAAGACCCAA TAGGTGATGT AGTGGAGGTT 
CAGCCGGTGA GTAACATGAG CACAGCCGCA GACCTCCTGA AACAGGGAGC AGCCTGTAAT 
GTGCTGTACC TGAATTCAGT GGAGACCGAG TCCCTGACGG GGCCACAGGC TATCGCTAAA 
GCTACAGGGG CCACATTGTC TCGCAGTCCT CGCCCCTCTG CCACAGTGGT CCACTTTAAG 
GTGTCTGCGC AAGGCATCAC ACTGACAGAC AGCCAGCGCA GGGTGTTCTT CAGGAGACAC 
TATCCCATCA ACAGTGTGAC TTACAGCAGC ATCGACCCAC AAGACAGGAG GTGGACTAAC 
TCAGACAGCA CGACCTCTAA AGTTTTCGGG TTTGTGGCCA AGAAGCCGGG CAGTGTGGCA 
GAGAATGTGT GCCATCTTTT CGCAGAGCTG GACCCTGAAC AGCCCGCGAC CGCCATCGTC 
AACTTCATCA ACAAAGTGAT GCTGGCACCG CAGAGGCGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294952.2
CDS	261..5042
Translation	MGCALSGDSCGDEPDPVPVRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_009296677.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296677.3

ORF Insert Sequence:

ATGGGTTGCG CCTTAAGTGG GGATAGCTGT GGGGACGAGC CAGATCCAGT ACCCGTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGACCTGC TCTGGGACCG CCAGCAAGGT 
CCACAGCACT ACCTGCATCG CACTTGTTCA GAGGGACCAT CTCGCCACCT GTATCCATAT 
CCATCCCAAG AGATCAATTC TCATCCACAC AGTCTGTCCC CCCGACTGCT TTGTAGACCA 
GATGAGTACA TTTCATATCC TCACCATCAT CACAATCATT CACACCATCC CAAACCCAGC 
GGACCCTACC ATGATGTCAT GATGATAGAT GGCCTAGTGA CACCCGGCTG CCCCTGTCGA 
GACTGTGCTC TACGACGAGA AGAGTTCCAC ACTTTACGTT TGGACAGAGA GGATGGAATA 
CACTGGGAGA GAGAGGCAGA GCTTCACCGG GATAATGGGT TAAGACGGAC AAGAGAGGCT 
GACATGCAGA GAGGGGCAGA GATATGGGAG CGGGAGCCAG GACTAAGGAG AGGAAGAGAG 
ATGTCTCTTC ACTGGGACAG AGACAGAGAT GCAGAACTAC AGTGGGAGAG AGAACGTGAG 
GCTGAATACT GGCACAGAAG GGCTACTGTA TCCCCATACG GGCCCCACGG TCATGAATTA 
CCTGCGTTTA ATTTTGATCC ACTCCCAGCA GGCCATCCTG CATACCCAGA ACCCTCCCGC 
TCTCACTCTC ATGCCCATCT GGATCTTAAA TACAGCAGCA GCTCCAGTGG CTACCAGACA 
CCACACCAGA CGTGCCCTTG CTCCCCCTAC CAGCCTTCAC CCTCAGAGAG TCGAGGATAT 
GCCTCGGGTT ACCAGTCTGA GTCTACGTCT CCTCTTCCGC CTCCATCTGC CTCCTGCCCC 
GGCCCGGTGC ACCATGCCAG TGGACCTACA GAAAACCAAA CTGACCCCCA TTCTCAGCAT 
TTCACCTCTG ACTCAAATAC AGATTGCCCT GGTGCAGATA GCATAGGATG GCGTAATCAT 
ATTACCCATG GATCCCTCCG GCGGATGCAT CCACATGCTC CATATTCAAC ACCCTCTGAT 
ATTTCAGGAC CTCCAACACC AGTCCACACA AGCAGCCCTT TACGAACCCA GGAAAGTCCA 
AGCCTGAGTG TCCGTGACTA TGACTCAAGA GAGGGGAACA GTGAAAGCAG TTCTGCTCAC 
TCCCTCAACA GGCCGCATCC AGCTACCATA CAGCCCAACA TGACCAATGC AGGATCACGG 
CAAACTCTAC TCTCTTCCAG TCCAGAGACT CCTGAGATTA ACCCGCTTCC TACCACACCC 
ACCACTGCCC AACATCTTGC AGCTCATTCC CTTCCTACAA CACCTCCAAC CCCACAAATT 
GCAACAGAAC ACTCACCCCC CTTAACGCCC GAGTCAGAAT CCACCCAACC ACAGGCAAAT 
TGCTCGCCTA CTGCTGCTAC AGTGCTGCCC ACAGTGGAAA TGGTGCCACA ATCAACTGAG 
ACTATAAAAC CAGCACATTC CCCACATCCC TCCTCTCCAG TCCTGGCTGA GTCCATTCCA 
GCTCACATGC AAAGCAATGG AGTACCACCA TCTCCTGAAT CAGATGCCCC TAAACCTGCT 
TCTTCAGCAC CTGCATCCCC TCAGCCACCT GCTGGTGACC ACGAGAGCTC TTCTTCCCCT 
GAACCTTCAG TGCCTGGATT TGCCACACTG GGCAGGAAGT TGATGTTGGG CTCTGAAACC 
TCACTTTCTG CAGGACACAG TATGGATGCC AGTTCTAATT CTACTCCCAC TTTCCCAGTC 
TCTCCTGCCT GCTGTACTCC ATCTCCTCCA CCAGGCTCCT ACCCAGGGTG CCCATCATCC 
TCCGTCCCAC AGCCTCCTCT CCCAGAGAAA CGCCGCCCAT CCGGCCTCCC AGGCTCACCC 
AATGGGAGGT CTGGAACACT GAGGGCATCC ATGAGTCACC AGCCCTCTGG GCAGCACCAT 
GTTACATTCT CGCCCTCAGT AGGGGAGCTG GCCGTACCCA TTGGTCAGGT TGAAGTTGGT 
GTGGAGGGTG AGATTGGCAA CCGTGTCAGT GTGAAATTTG TGCAGGACAG CTCACGATTT 
TGGTATAAAC CTGGCATCTC CAGAGAGCAA GCTATAGCAG CACTGAAGGA AAGAGAGCCA 
GGGGCCTTCC TCATCCGAGA CAGTAACTCC TTCCAGGGGG CCTACGGTCT GGCCCTGAAA 
GTTGCCAGCC CACCCCCCAA CGTCAATAAT CATAGCAGCA AAGGAGACCC TCTGGAACAG 
CTGGTTCGTC ACTTTCTGAT TGAGACGGGG CCGCGAGGGG TGAAGATCAA GGGCTGTCAG 
AACGAGCCCT ACTTTGGGAG TCTTTCAGCA CTGGTGTATC AGCATTCCAT TACACCCATC 
TCTCTGCCCT GTGCCCTCCG TATCCCAGAG AAAGACCCAA TAGGTGATGT AGTGGAGGTT 
CAGCCGGTGA GTAACATGAG CACAGCCGCA GACCTCCTGA AACAGGGAGC AGCCTGTAAT 
GTGCTGTACC TGAATTCAGT GGAGACCGAG TCCCTGACGG GGCCACAGGC TATCGCTAAA 
GCTACAGGGG CCACATTGTC TCGCAGTCCT CGCCCCTCTG CCACAGTGGT CCACTTTAAG 
GTGTCTGCGC AAGGCATCAC ACTGACAGAC AGCCAGCGCA GGGTGTTCTT CAGGAGACAC 
TATCCCATCA ACAGTGTGAC TTACAGCAGC ATCGACCCAC AAGACAGGAG GTGGACTAAC 
TCAGACAGCA CGACCTCTAA AGTTTTCGGG TTTGTGGCCA AGAAGCCGGG CAGTGTGGCA 
GAGAATGTGT GCCATCTTTT CGCAGAGCTG GACCCTGAAC AGCCCGCGAC CGCCATCGTC 
AACTTCATCA ACAAAGTGAT GCTGGCACCG CAGAGGCGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_021469904.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325579.1 Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.

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CloneID	ODa27477
Clone ID Related Accession (Same CDS sequence)	XM_021469904.1 , XM_001921008.4
Accession Version	XM_021469904.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGG 
TGGACTAACT CAGACAGCAC GACCTCTAAA GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC 
AGTGTGGCAG AGAATGTGTG CCATCTTTTC GCAGAGCTGG ACCCTGAACA GCCCGCGACC 
GCCATCGTCA ACTTCATCAA CAAAGTGATG CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325579.1
CDS	259..5109
Translation	MGCALSGDSCGDEPDPVPLRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_021469904.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469904.1

ORF Insert Sequence:

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGG 
TGGACTAACT CAGACAGCAC GACCTCTAAA GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC 
AGTGTGGCAG AGAATGTGTG CCATCTTTTC GCAGAGCTGG ACCCTGAACA GCCCGCGACC 
GCCATCGTCA ACTTCATCAA CAAAGTGATG CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_001921008.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_001921043.2 Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.

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CloneID	ODa27477
Clone ID Related Accession (Same CDS sequence)	XM_021469904.1 , XM_001921008.4
Accession Version	XM_001921008.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4851bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001921008.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGG 
TGGACTAACT CAGACAGCAC GACCTCTAAA GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC 
AGTGTGGCAG AGAATGTGTG CCATCTTTTC GCAGAGCTGG ACCCTGAACA GCCCGCGACC 
GCCATCGTCA ACTTCATCAA CAAAGTGATG CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001921043.2
CDS	250..5100
Translation	MGCALSGDSCGDEPDPVPLRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_001921008.4
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001921008.4

ORF Insert Sequence:

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGG 
TGGACTAACT CAGACAGCAC GACCTCTAAA GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC 
AGTGTGGCAG AGAATGTGTG CCATCTTTTC GCAGAGCTGG ACCCTGAACA GCCCGCGACC 
GCCATCGTCA ACTTCATCAA CAAAGTGATG CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_021469905.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325580.1 Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27478
Clone ID Related Accession (Same CDS sequence)	XM_021469905.1 , XM_005161970.3
Accession Version	XM_021469905.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGA 
GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC AGTGTGGCAG AGAATGTGTG CCATCTTTTC 
GCAGAGCTGG ACCCTGAACA GCCCGCGACC GCCATCGTCA ACTTCATCAA CAAAGTGATG 
CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325580.1
CDS	259..5079
Translation	MGCALSGDSCGDEPDPVPLRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_021469905.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469905.1

ORF Insert Sequence:

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGA 
GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC AGTGTGGCAG AGAATGTGTG CCATCTTTTC 
GCAGAGCTGG ACCCTGAACA GCCCGCGACC GCCATCGTCA ACTTCATCAA CAAAGTGATG 
CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_005161970.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162027.1 Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27478
Clone ID Related Accession (Same CDS sequence)	XM_021469905.1 , XM_005161970.3
Accession Version	XM_005161970.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161970.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGA 
GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC AGTGTGGCAG AGAATGTGTG CCATCTTTTC 
GCAGAGCTGG ACCCTGAACA GCCCGCGACC GCCATCGTCA ACTTCATCAA CAAAGTGATG 
CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162027.1
CDS	250..5070
Translation	MGCALSGDSCGDEPDPVPLRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_005161970.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161970.3

ORF Insert Sequence:

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGGTATGT CATCTGTCAT AGGACTTTGC 
CAACATCACA GCACCCATCC TCACCAATCC CTTCCTCCTC CAGACCTGCT CTGGGACCGC 
CAGCAAGGTC CACAGCACTA CCTGCATCGC ACTTGTTCAG AGGGACCATC TCGCCACCTG 
TATCCATATC CATCCCAAGA GATCAATTCT CATCCACACA GTCTGTCCCC CCGACTGCTT 
TGTAGACCAG ATGAGTACAT TTCATATCCT CACCATCATC ACAATCATTC ACACCATCCC 
AAACCCAGCG GACCCTACCA TGATGTCATG ATGATAGATG GCCTAGTGAC ACCCGGCTGC 
CCCTGTCGAG ACTGTGCTCT ACGACGAGAA GAGTTCCACA CTTTACGTTT GGACAGAGAG 
GATGGAATAC ACTGGGAGAG AGAGGCAGAG CTTCACCGGG ATAATGGGTT AAGACGGACA 
AGAGAGGCTG ACATGCAGAG AGGGGCAGAG ATATGGGAGC GGGAGCCAGG ACTAAGGAGA 
GGAAGAGAGA TGTCTCTTCA CTGGGACAGA GACAGAGATG CAGAACTACA GTGGGAGAGA 
GAACGTGAGG CTGAATACTG GCACAGAAGG GCTACTGTAT CCCCATACGG GCCCCACGGT 
CATGAATTAC CTGCGTTTAA TTTTGATCCA CTCCCAGCAG GCCATCCTGC ATACCCAGAA 
CCCTCCCGCT CTCACTCTCA TGCCCATCTG GATCTTAAAT ACAGCAGCAG CTCCAGTGGC 
TACCAGACAC CACACCAGAC GTGCCCTTGC TCCCCCTACC AGCCTTCACC CTCAGAGAGT 
CGAGGATATG CCTCGGGTTA CCAGTCTGAG TCTACGTCTC CTCTTCCGCC TCCATCTGCC 
TCCTGCCCCG GCCCGGTGCA CCATGCCAGT GGACCTACAG AAAACCAAAC TGACCCCCAT 
TCTCAGCATT TCACCTCTGA CTCAAATACA GATTGCCCTG GTGCAGATAG CATAGGATGG 
CGTAATCATA TTACCCATGG ATCCCTCCGG CGGATGCATC CACATGCTCC ATATTCAACA 
CCCTCTGATA TTTCAGGACC TCCAACACCA GTCCACACAA GCAGCCCTTT ACGAACCCAG 
GAAAGTCCAA GCCTGAGTGT CCGTGACTAT GACTCAAGAG AGGGGAACAG TGAAAGCAGT 
TCTGCTCACT CCCTCAACAG GCCGCATCCA GCTACCATAC AGCCCAACAT GACCAATGCA 
GGATCACGGC AAACTCTACT CTCTTCCAGT CCAGAGACTC CTGAGATTAA CCCGCTTCCT 
ACCACACCCA CCACTGCCCA ACATCTTGCA GCTCATTCCC TTCCTACAAC ACCTCCAACC 
CCACAAATTG CAACAGAACA CTCACCCCCC TTAACGCCCG AGTCAGAATC CACCCAACCA 
CAGGCAAATT GCTCGCCTAC TGCTGCTACA GTGCTGCCCA CAGTGGAAAT GGTGCCACAA 
TCAACTGAGA CTATAAAACC AGCACATTCC CCACATCCCT CCTCTCCAGT CCTGGCTGAG 
TCCATTCCAG CTCACATGCA AAGCAATGGA GTACCACCAT CTCCTGAATC AGATGCCCCT 
AAACCTGCTT CTTCAGCACC TGCATCCCCT CAGCCACCTG CTGGTGACCA CGAGAGCTCT 
TCTTCCCCTG AACCTTCAGT GCCTGGATTT GCCACACTGG GCAGGAAGTT GATGTTGGGC 
TCTGAAACCT CACTTTCTGC AGGACACAGT ATGGATGCCA GTTCTAATTC TACTCCCACT 
TTCCCAGTCT CTCCTGCCTG CTGTACTCCA TCTCCTCCAC CAGGCTCCTA CCCAGGGTGC 
CCATCATCCT CCGTCCCACA GCCTCCTCTC CCAGAGAAAC GCCGCCCATC CGGCCTCCCA 
GGCTCACCCA ATGGGAGGTC TGGAACACTG AGGGCATCCA TGAGTCACCA GCCCTCTGGG 
CAGCACCATG TTACATTCTC GCCCTCAGTA GGGGAGCTGG CCGTACCCAT TGGTCAGGTT 
GAAGTTGGTG TGGAGGGTGA GATTGGCAAC CGTGTCAGTG TGAAATTTGT GCAGGACAGC 
TCACGATTTT GGTATAAACC TGGCATCTCC AGAGAGCAAG CTATAGCAGC ACTGAAGGAA 
AGAGAGCCAG GGGCCTTCCT CATCCGAGAC AGTAACTCCT TCCAGGGGGC CTACGGTCTG 
GCCCTGAAAG TTGCCAGCCC ACCCCCCAAC GTCAATAATC ATAGCAGCAA AGGAGACCCT 
CTGGAACAGC TGGTTCGTCA CTTTCTGATT GAGACGGGGC CGCGAGGGGT GAAGATCAAG 
GGCTGTCAGA ACGAGCCCTA CTTTGGGAGT CTTTCAGCAC TGGTGTATCA GCATTCCATT 
ACACCCATCT CTCTGCCCTG TGCCCTCCGT ATCCCAGAGA AAGACCCAAT AGGTGATGTA 
GTGGAGGTTC AGCCGGTGAG TAACATGAGC ACAGCCGCAG ACCTCCTGAA ACAGGGAGCA 
GCCTGTAATG TGCTGTACCT GAATTCAGTG GAGACCGAGT CCCTGACGGG GCCACAGGCT 
ATCGCTAAAG CTACAGGGGC CACATTGTCT CGCAGTCCTC GCCCCTCTGC CACAGTGGTC 
CACTTTAAGG TGTCTGCGCA AGGCATCACA CTGACAGACA GCCAGCGCAG GGTGTTCTTC 
AGGAGACACT ATCCCATCAA CAGTGTGACT TACAGCAGCA TCGACCCACA AGACAGGAGA 
GTTTTCGGGT TTGTGGCCAA GAAGCCGGGC AGTGTGGCAG AGAATGTGTG CCATCTTTTC 
GCAGAGCTGG ACCCTGAACA GCCCGCGACC GCCATCGTCA ACTTCATCAA CAAAGTGATG 
CTGGCACCGC AGAGGCGCTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_005161971.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162028.1 Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.

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CloneID	ODa27479
Clone ID Related Accession (Same CDS sequence)	XM_005161971.3 , XM_021469906.1 , XM_005161971.4
Accession Version	XM_005161971.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005161971.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TCAGGAAAAC CTTCTTAGCT GGAATATTCT CCAACAACAT GAACAAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162028.1
CDS	55..4848
Translation	MTSFRKTFLAGIFSNNMNKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_005161971.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161971.3

ORF Insert Sequence:

ATGACATCTT TCAGGAAAAC CTTCTTAGCT GGAATATTCT CCAACAACAT GAACAAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_021469906.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325581.1 Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.

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CloneID	ODa27479
Clone ID Related Accession (Same CDS sequence)	XM_005161971.3 , XM_021469906.1 , XM_005161971.4
Accession Version	XM_021469906.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TCAGGAAAAC CTTCTTAGCT GGAATATTCT CCAACAACAT GAACAAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325581.1
CDS	184..4977
Translation	MTSFRKTFLAGIFSNNMNKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_021469906.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469906.1

ORF Insert Sequence:

ATGACATCTT TCAGGAAAAC CTTCTTAGCT GGAATATTCT CCAACAACAT GAACAAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_005161971.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005162028.1 Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.

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CloneID	ODa27479
Clone ID Related Accession (Same CDS sequence)	XM_005161971.3 , XM_021469906.1 , XM_005161971.4
Accession Version	XM_005161971.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395129.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005161971.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACATCTT TCAGGAAAAC CTTCTTAGCT GGAATATTCT CCAACAACAT GAACAAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005162028.1
CDS	20..4813
Translation	MTSFRKTFLAGIFSNNMNKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_005161971.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005161971.4

ORF Insert Sequence:

ATGACATCTT TCAGGAAAAC CTTCTTAGCT GGAATATTCT CCAACAACAT GAACAAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_009296675.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294950.1 Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa27480
Clone ID Related Accession (Same CDS sequence)	XM_009296675.2 , XM_009296676.2 , XM_009296675.3 , XM_009296676.3
Accession Version	XM_009296675.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296675.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294950.1
CDS	121..4914
Translation	MSCCSWKERQRPEFHRTVQAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_009296675.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296675.2

ORF Insert Sequence:

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_009296676.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294951.1 Danio rerio tensin 2a (tns2a), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa27480
Clone ID Related Accession (Same CDS sequence)	XM_009296675.2 , XM_009296676.2 , XM_009296675.3 , XM_009296676.3
Accession Version	XM_009296676.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-2 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296676.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294951.1
CDS	49..4842
Translation	MSCCSWKERQRPEFHRTVQAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_009296676.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296676.2

ORF Insert Sequence:

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_009296675.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294950.1 Danio rerio tensin 2a (tns2a), transcript variant X5, mRNA.

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CloneID	ODa27480
Clone ID Related Accession (Same CDS sequence)	XM_009296675.2 , XM_009296676.2 , XM_009296675.3 , XM_009296676.3
Accession Version	XM_009296675.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296675.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294950.1
CDS	250..5043
Translation	MSCCSWKERQRPEFHRTVQAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_009296675.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296675.3

ORF Insert Sequence:

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_009296676.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294951.1 Danio rerio tensin 2a (tns2a), transcript variant X6, mRNA.

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CloneID	ODa27480
Clone ID Related Accession (Same CDS sequence)	XM_009296675.2 , XM_009296676.2 , XM_009296675.3 , XM_009296676.3
Accession Version	XM_009296676.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4794bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296676.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294951.1
CDS	108..4901
Translation	MSCCSWKERQRPEFHRTVQAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPGMSSVIGLCQHHSTHPHQSLPPPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_009296676.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296676.3

ORF Insert Sequence:

ATGAGCTGCT GTTCATGGAA AGAGAGACAG AGGCCTGAAT TCCACAGAAC AGTTCAGGCG 
GGGAAGGGCG AGCCACACGT CTTCAAAGAA AAGGCCTTCA AAAAGAAGCG CCAATGTGGA 
GTGTGCAGGC AGAGCGTGGA GAACCTGGGC TCTTTTTGTC GAGTCTGCAA AACAGCCACC 
CATAAGAAAT GCGAAGCCAA GATCAACACG GCCTGCATCC CAGCGCAGCC CTCAGAGCTG 
CGAAGGGGAA CATCCTCCTC TAGACACATT CAGCATCTGG GCTCAACCAA GTCTCTGACC 
TACACCAAAC AGAGGAACAC TCTCCCGAGG AGCTTCAGTG TGGATCGGGT GATGGACAGA 
GTGATGGAGA GACACTATGA TTTCGACCTC ACATACATCA CAGAGAGGAT CATCTCAGTC 
TTCTTTCCTC CTCTGCTGGA GGAACAGCGC TACCGCCTCA ACCTCAGAGA GGTGGCAGCC 
ATGCTCAAGT CCAAACACCA AGACAAGTTT CTGTTATTAA ATCTGTCTGA GCGAAGGCAT 
GACATCACCC GCTTAAACCC AAAGGTTCAT GACTTCGGCT GGCCAGATAT GCACTCTCCT 
CCGCTGGATA AGATCTGTGC CATGTGCAAA GCCATGGAGA ACTGGCTGAA CTCTGACCCC 
CAGCATGTGG TCGTTCTGCA CTGTAAGGGC AACAAAGGAA AGACCGGTGT AATCATTGCT 
GCCTATATGC ATTACAGCAA GATATCAGCG GGGGCAGATC AGGCGCTCAG CACGTTGGCC 
ATGAGGAAGT TTTGTGAAGA TAAAGTAGCG TCCTCTCTCC AGCCCTCTCA GAACAGGTAT 
ATCCAGTACT TTGGAGGGCT TCTCTCAGGG TCTATCAAAA TGAACAGCAG TCCTCTCTTC 
CTCCACCAGG TCCTCATTCC TACCATCCCC AACTTCCAGG AAGATGATGG TTTCTTCCCT 
TTCCTGAAGA TCTATCAGTC CATGCAGCTT GTCTATACTT CTGGCATATA TGATCTGCAG 
GGCTCTGCGA GCAGGCGGCT GATCATTACT ATTGAGCCTG CATTGCTCCT GAAAGGAGAC 
ATCATGGTGA AGTGTTATCA CAGGCGAGTG GAGTCTGCTG AGAGAGACTC AGTGTTTCGA 
CTGCAGTTTC ATACATGCAC CGTTCATGCA GCTCAGCTCT GGTTCGGCAA AGGGGAACTG 
GATGAAGCTT GTTCAGATGA CAAATTTCCC CCTGATGCCA TAGTGGAGTT TGTGTTTTCA 
TCTGGGCCTG AGAAAATTAA AGGGAGAGTG AGCCAAAGGA ATGATCCAGC TGTCAGTGTG 
GACTATAACA CAAATGATCC AGTGGTGCGC TGGGACTCAT ATGAGAACTT CAATCAGAGG 
CATCAGGACA GCCTAGAGGA TATTGCTCAT ACACGTGGCC CTGTAGATGG CAGCTTGTAT 
GCACAGGTTA AAAAGCGCCG CGCTGCTGCT GGCTGCTCCT CTCTGCCATC GACCAATGGA 
AGCCCTGCAA GAAGCTCTGA GGAAAGACCA AGTCAGCTTT TGTCTGTTAG CACTGATTCC 
GGACACTCAT CTGTTCCTCC TGATCGACTC GATGAACCGA CTCGACCGGC CCCGCCTACT 
CAGCAAGAGA GGGAGGAGCT TGAGCGGCTA TTGGGTGGGA TTGAAGGCGA TGGACTGAGA 
GACCGTGACC GAGAGACGGC TATTTTGGAT GATGGTGATT CCTTGCCTCC TGAAAGAACT 
GGGTCTCTGA GGTTAGGCCG TTCATGTTCT TGTCGTGTGG GTTACCGATC TCAGGGTTGT 
GCTGAGCCTG GATGTAATGG ATTTCATCAT TTACCCAATG GCCATTGCCT TGATCGGCCC 
ACCACCACTA ATGGACACAC AGGGGCTCTG TCTCCAGGTA TGTCATCTGT CATAGGACTT 
TGCCAACATC ACAGCACCCA TCCTCACCAA TCCCTTCCTC CTCCAGACCT GCTCTGGGAC 
CGCCAGCAAG GTCCACAGCA CTACCTGCAT CGCACTTGTT CAGAGGGACC ATCTCGCCAC 
CTGTATCCAT ATCCATCCCA AGAGATCAAT TCTCATCCAC ACAGTCTGTC CCCCCGACTG 
CTTTGTAGAC CAGATGAGTA CATTTCATAT CCTCACCATC ATCACAATCA TTCACACCAT 
CCCAAACCCA GCGGACCCTA CCATGATGTC ATGATGATAG ATGGCCTAGT GACACCCGGC 
TGCCCCTGTC GAGACTGTGC TCTACGACGA GAAGAGTTCC ACACTTTACG TTTGGACAGA 
GAGGATGGAA TACACTGGGA GAGAGAGGCA GAGCTTCACC GGGATAATGG GTTAAGACGG 
ACAAGAGAGG CTGACATGCA GAGAGGGGCA GAGATATGGG AGCGGGAGCC AGGACTAAGG 
AGAGGAAGAG AGATGTCTCT TCACTGGGAC AGAGACAGAG ATGCAGAACT ACAGTGGGAG 
AGAGAACGTG AGGCTGAATA CTGGCACAGA AGGGCTACTG TATCCCCATA CGGGCCCCAC 
GGTCATGAAT TACCTGCGTT TAATTTTGAT CCACTCCCAG CAGGCCATCC TGCATACCCA 
GAACCCTCCC GCTCTCACTC TCATGCCCAT CTGGATCTTA AATACAGCAG CAGCTCCAGT 
GGCTACCAGA CACCACACCA GACGTGCCCT TGCTCCCCCT ACCAGCCTTC ACCCTCAGAG 
AGTCGAGGAT ATGCCTCGGG TTACCAGTCT GAGTCTACGT CTCCTCTTCC GCCTCCATCT 
GCCTCCTGCC CCGGCCCGGT GCACCATGCC AGTGGACCTA CAGAAAACCA AACTGACCCC 
CATTCTCAGC ATTTCACCTC TGACTCAAAT ACAGATTGCC CTGGTGCAGA TAGCATAGGA 
TGGCGTAATC ATATTACCCA TGGATCCCTC CGGCGGATGC ATCCACATGC TCCATATTCA 
ACACCCTCTG ATATTTCAGG ACCTCCAACA CCAGTCCACA CAAGCAGCCC TTTACGAACC 
CAGGAAAGTC CAAGCCTGAG TGTCCGTGAC TATGACTCAA GAGAGGGGAA CAGTGAAAGC 
AGTTCTGCTC ACTCCCTCAA CAGGCCGCAT CCAGCTACCA TACAGCCCAA CATGACCAAT 
GCAGGATCAC GGCAAACTCT ACTCTCTTCC AGTCCAGAGA CTCCTGAGAT TAACCCGCTT 
CCTACCACAC CCACCACTGC CCAACATCTT GCAGCTCATT CCCTTCCTAC AACACCTCCA 
ACCCCACAAA TTGCAACAGA ACACTCACCC CCCTTAACGC CCGAGTCAGA ATCCACCCAA 
CCACAGGCAA ATTGCTCGCC TACTGCTGCT ACAGTGCTGC CCACAGTGGA AATGGTGCCA 
CAATCAACTG AGACTATAAA ACCAGCACAT TCCCCACATC CCTCCTCTCC AGTCCTGGCT 
GAGTCCATTC CAGCTCACAT GCAAAGCAAT GGAGTACCAC CATCTCCTGA ATCAGATGCC 
CCTAAACCTG CTTCTTCAGC ACCTGCATCC CCTCAGCCAC CTGCTGGTGA CCACGAGAGC 
TCTTCTTCCC CTGAACCTTC AGTGCCTGGA TTTGCCACAC TGGGCAGGAA GTTGATGTTG 
GGCTCTGAAA CCTCACTTTC TGCAGGACAC AGTATGGATG CCAGTTCTAA TTCTACTCCC 
ACTTTCCCAG TCTCTCCTGC CTGCTGTACT CCATCTCCTC CACCAGGCTC CTACCCAGGG 
TGCCCATCAT CCTCCGTCCC ACAGCCTCCT CTCCCAGAGA AACGCCGCCC ATCCGGCCTC 
CCAGGCTCAC CCAATGGGAG GTCTGGAACA CTGAGGGCAT CCATGAGTCA CCAGCCCTCT 
GGGCAGCACC ATGTTACATT CTCGCCCTCA GTAGGGGAGC TGGCCGTACC CATTGGTCAG 
GTTGAAGTTG GTGTGGAGGG TGAGATTGGC AACCGTGTCA GTGTGAAATT TGTGCAGGAC 
AGCTCACGAT TTTGGTATAA ACCTGGCATC TCCAGAGAGC AAGCTATAGC AGCACTGAAG 
GAAAGAGAGC CAGGGGCCTT CCTCATCCGA GACAGTAACT CCTTCCAGGG GGCCTACGGT 
CTGGCCCTGA AAGTTGCCAG CCCACCCCCC AACGTCAATA ATCATAGCAG CAAAGGAGAC 
CCTCTGGAAC AGCTGGTTCG TCACTTTCTG ATTGAGACGG GGCCGCGAGG GGTGAAGATC 
AAGGGCTGTC AGAACGAGCC CTACTTTGGG AGTCTTTCAG CACTGGTGTA TCAGCATTCC 
ATTACACCCA TCTCTCTGCC CTGTGCCCTC CGTATCCCAG AGAAAGACCC AATAGGTGAT 
GTAGTGGAGG TTCAGCCGGT GAGTAACATG AGCACAGCCG CAGACCTCCT GAAACAGGGA 
GCAGCCTGTA ATGTGCTGTA CCTGAATTCA GTGGAGACCG AGTCCCTGAC GGGGCCACAG 
GCTATCGCTA AAGCTACAGG GGCCACATTG TCTCGCAGTC CTCGCCCCTC TGCCACAGTG 
GTCCACTTTA AGGTGTCTGC GCAAGGCATC ACACTGACAG ACAGCCAGCG CAGGGTGTTC 
TTCAGGAGAC ACTATCCCAT CAACAGTGTG ACTTACAGCA GCATCGACCC ACAAGACAGG 
AGGTGGACTA ACTCAGACAG CACGACCTCT AAAGTTTTCG GGTTTGTGGC CAAGAAGCCG 
GGCAGTGTGG CAGAGAATGT GTGCCATCTT TTCGCAGAGC TGGACCCTGA ACAGCCCGCG 
ACCGCCATCG TCAACTTCAT CAACAAAGTG ATGCTGGCAC CGCAGAGGCG CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_009296677.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294952.1 Danio rerio tensin 2a (tns2a), transcript variant X6, mRNA.

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CloneID	ODa47336
Clone ID Related Accession (Same CDS sequence)	XM_009296677.2 , XM_021469907.1
Accession Version	XM_009296677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	tensin-2 isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296677.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGACCTGC TCTGGGACCG CCAGCAAGGT 
CCACAGCACT ACCTGCATCG CACTTGTTCA GAGGGACCAT CTCGCCACCT GTATCCATAT 
CCATCCCAAG AGATCAATTC TCATCCACAC AGTCTGTCCC CCCGACTGCT TTGTAGACCA 
GATGAGTACA TTTCATATCC TCACCATCAT CACAATCATT CACACCATCC CAAACCCAGC 
GGACCCTACC ATGATGTCAT GATGATAGAT GGCCTAGTGA CACCCGGCTG CCCCTGTCGA 
GACTGTGCTC TACGACGAGA AGAGTTCCAC ACTTTACGTT TGGACAGAGA GGATGGAATA 
CACTGGGAGA GAGAGGCAGA GCTTCACCGG GATAATGGGT TAAGACGGAC AAGAGAGGCT 
GACATGCAGA GAGGGGCAGA GATATGGGAG CGGGAGCCAG GACTAAGGAG AGGAAGAGAG 
ATGTCTCTTC ACTGGGACAG AGACAGAGAT GCAGAACTAC AGTGGGAGAG AGAACGTGAG 
GCTGAATACT GGCACAGAAG GGCTACTGTA TCCCCATACG GGCCCCACGG TCATGAATTA 
CCTGCGTTTA ATTTTGATCC ACTCCCAGCA GGCCATCCTG CATACCCAGA ACCCTCCCGC 
TCTCACTCTC ATGCCCATCT GGATCTTAAA TACAGCAGCA GCTCCAGTGG CTACCAGACA 
CCACACCAGA CGTGCCCTTG CTCCCCCTAC CAGCCTTCAC CCTCAGAGAG TCGAGGATAT 
GCCTCGGGTT ACCAGTCTGA GTCTACGTCT CCTCTTCCGC CTCCATCTGC CTCCTGCCCC 
GGCCCGGTGC ACCATGCCAG TGGACCTACA GAAAACCAAA CTGACCCCCA TTCTCAGCAT 
TTCACCTCTG ACTCAAATAC AGATTGCCCT GGTGCAGATA GCATAGGATG GCGTAATCAT 
ATTACCCATG GATCCCTCCG GCGGATGCAT CCACATGCTC CATATTCAAC ACCCTCTGAT 
ATTTCAGGAC CTCCAACACC AGTCCACACA AGCAGCCCTT TACGAACCCA GGAAAGTCCA 
AGCCTGAGTG TCCGTGACTA TGACTCAAGA GAGGGGAACA GTGAAAGCAG TTCTGCTCAC 
TCCCTCAACA GGCCGCATCC AGCTACCATA CAGCCCAACA TGACCAATGC AGGATCACGG 
CAAACTCTAC TCTCTTCCAG TCCAGAGACT CCTGAGATTA ACCCGCTTCC TACCACACCC 
ACCACTGCCC AACATCTTGC AGCTCATTCC CTTCCTACAA CACCTCCAAC CCCACAAATT 
GCAACAGAAC ACTCACCCCC CTTAACGCCC GAGTCAGAAT CCACCCAACC ACAGGCAAAT 
TGCTCGCCTA CTGCTGCTAC AGTGCTGCCC ACAGTGGAAA TGGTGCCACA ATCAACTGAG 
ACTATAAAAC CAGCACATTC CCCACATCCC TCCTCTCCAG TCCTGGCTGA GTCCATTCCA 
GCTCACATGC AAAGCAATGG AGTACCACCA TCTCCTGAAT CAGATGCCCC TAAACCTGCT 
TCTTCAGCAC CTGCATCCCC TCAGCCACCT GCTGGTGACC ACGAGAGCTC TTCTTCCCCT 
GAACCTTCAG TGCCTGGATT TGCCACACTG GGCAGGAAGT TGATGTTGGG CTCTGAAACC 
TCACTTTCTG CAGGACACAG TATGGATGCC AGTTCTAATT CTACTCCCAC TTTCCCAGTC 
TCTCCTGCCT GCTGTACTCC ATCTCCTCCA CCAGGCTCCT ACCCAGGGTG CCCATCATCC 
TCCGTCCCAC AGCCTCCTCT CCCAGAGAAA CGCCGCCCAT CCGGCCTCCC AGGCTCACCC 
AATGGGAGGT CTGGAACACT GAGGGCATCC ATGAGTCACC AGCCCTCTGG GCAGCACCAT 
GTTACATTCT CGCCCTCAGT AGGGGAGCTG GCCGTACCCA TTGGTCAGGT TGAAGTTGGT 
GTGGAGGGTG AGATTGGCAA CCGTGTCAGT GTGAAATTTG TGCAGGACAG CTCACGATTT 
TGGTATAAAC CTGGCATCTC CAGAGAGCAA GCTATAGCAG CACTGAAGGA AAGAGAGCCA 
GGGGCCTTCC TCATCCGAGA CAGTAACTCC TTCCAGGGGG CCTACGGTCT GGCCCTGAAA 
GTTGCCAGCC CACCCCCCAA CGTCAATAAT CATAGCAGCA AAGGAGACCC TCTGGAACAG 
CTGGTTCGTC ACTTTCTGAT TGAGACGGGG CCGCGAGGGG TGAAGATCAA GGGCTGTCAG 
AACGAGCCCT ACTTTGGGAG TCTTTCAGCA CTGGTGTATC AGCATTCCAT TACACCCATC 
TCTCTGCCCT GTGCCCTCCG TATCCCAGAG AAAGACCCAA TAGGTGATGT AGTGGAGGTT 
CAGCCGGTGA GTAACATGAG CACAGCCGCA GACCTCCTGA AACAGGGAGC AGCCTGTAAT 
GTGCTGTACC TGAATTCAGT GGAGACCGAG TCCCTGACGG GGCCACAGGC TATCGCTAAA 
GCTACAGGGG CCACATTGTC TCGCAGTCCT CGCCCCTCTG CCACAGTGGT CCACTTTAAG 
GTGTCTGCGC AAGGCATCAC ACTGACAGAC AGCCAGCGCA GGGTGTTCTT CAGGAGACAC 
TATCCCATCA ACAGTGTGAC TTACAGCAGC ATCGACCCAC AAGACAGGAG GTGGACTAAC 
TCAGACAGCA CGACCTCTAA AGTTTTCGGG TTTGTGGCCA AGAAGCCGGG CAGTGTGGCA 
GAGAATGTGT GCCATCTTTT CGCAGAGCTG GACCCTGAAC AGCCCGCGAC CGCCATCGTC 
AACTTCATCA ACAAAGTGAT GCTGGCACCG CAGAGGCGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294952.1
CDS	251..5032
Translation	MGCALSGDSCGDEPDPVPLRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_009296677.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296677.2

ORF Insert Sequence:

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGACCTGC TCTGGGACCG CCAGCAAGGT 
CCACAGCACT ACCTGCATCG CACTTGTTCA GAGGGACCAT CTCGCCACCT GTATCCATAT 
CCATCCCAAG AGATCAATTC TCATCCACAC AGTCTGTCCC CCCGACTGCT TTGTAGACCA 
GATGAGTACA TTTCATATCC TCACCATCAT CACAATCATT CACACCATCC CAAACCCAGC 
GGACCCTACC ATGATGTCAT GATGATAGAT GGCCTAGTGA CACCCGGCTG CCCCTGTCGA 
GACTGTGCTC TACGACGAGA AGAGTTCCAC ACTTTACGTT TGGACAGAGA GGATGGAATA 
CACTGGGAGA GAGAGGCAGA GCTTCACCGG GATAATGGGT TAAGACGGAC AAGAGAGGCT 
GACATGCAGA GAGGGGCAGA GATATGGGAG CGGGAGCCAG GACTAAGGAG AGGAAGAGAG 
ATGTCTCTTC ACTGGGACAG AGACAGAGAT GCAGAACTAC AGTGGGAGAG AGAACGTGAG 
GCTGAATACT GGCACAGAAG GGCTACTGTA TCCCCATACG GGCCCCACGG TCATGAATTA 
CCTGCGTTTA ATTTTGATCC ACTCCCAGCA GGCCATCCTG CATACCCAGA ACCCTCCCGC 
TCTCACTCTC ATGCCCATCT GGATCTTAAA TACAGCAGCA GCTCCAGTGG CTACCAGACA 
CCACACCAGA CGTGCCCTTG CTCCCCCTAC CAGCCTTCAC CCTCAGAGAG TCGAGGATAT 
GCCTCGGGTT ACCAGTCTGA GTCTACGTCT CCTCTTCCGC CTCCATCTGC CTCCTGCCCC 
GGCCCGGTGC ACCATGCCAG TGGACCTACA GAAAACCAAA CTGACCCCCA TTCTCAGCAT 
TTCACCTCTG ACTCAAATAC AGATTGCCCT GGTGCAGATA GCATAGGATG GCGTAATCAT 
ATTACCCATG GATCCCTCCG GCGGATGCAT CCACATGCTC CATATTCAAC ACCCTCTGAT 
ATTTCAGGAC CTCCAACACC AGTCCACACA AGCAGCCCTT TACGAACCCA GGAAAGTCCA 
AGCCTGAGTG TCCGTGACTA TGACTCAAGA GAGGGGAACA GTGAAAGCAG TTCTGCTCAC 
TCCCTCAACA GGCCGCATCC AGCTACCATA CAGCCCAACA TGACCAATGC AGGATCACGG 
CAAACTCTAC TCTCTTCCAG TCCAGAGACT CCTGAGATTA ACCCGCTTCC TACCACACCC 
ACCACTGCCC AACATCTTGC AGCTCATTCC CTTCCTACAA CACCTCCAAC CCCACAAATT 
GCAACAGAAC ACTCACCCCC CTTAACGCCC GAGTCAGAAT CCACCCAACC ACAGGCAAAT 
TGCTCGCCTA CTGCTGCTAC AGTGCTGCCC ACAGTGGAAA TGGTGCCACA ATCAACTGAG 
ACTATAAAAC CAGCACATTC CCCACATCCC TCCTCTCCAG TCCTGGCTGA GTCCATTCCA 
GCTCACATGC AAAGCAATGG AGTACCACCA TCTCCTGAAT CAGATGCCCC TAAACCTGCT 
TCTTCAGCAC CTGCATCCCC TCAGCCACCT GCTGGTGACC ACGAGAGCTC TTCTTCCCCT 
GAACCTTCAG TGCCTGGATT TGCCACACTG GGCAGGAAGT TGATGTTGGG CTCTGAAACC 
TCACTTTCTG CAGGACACAG TATGGATGCC AGTTCTAATT CTACTCCCAC TTTCCCAGTC 
TCTCCTGCCT GCTGTACTCC ATCTCCTCCA CCAGGCTCCT ACCCAGGGTG CCCATCATCC 
TCCGTCCCAC AGCCTCCTCT CCCAGAGAAA CGCCGCCCAT CCGGCCTCCC AGGCTCACCC 
AATGGGAGGT CTGGAACACT GAGGGCATCC ATGAGTCACC AGCCCTCTGG GCAGCACCAT 
GTTACATTCT CGCCCTCAGT AGGGGAGCTG GCCGTACCCA TTGGTCAGGT TGAAGTTGGT 
GTGGAGGGTG AGATTGGCAA CCGTGTCAGT GTGAAATTTG TGCAGGACAG CTCACGATTT 
TGGTATAAAC CTGGCATCTC CAGAGAGCAA GCTATAGCAG CACTGAAGGA AAGAGAGCCA 
GGGGCCTTCC TCATCCGAGA CAGTAACTCC TTCCAGGGGG CCTACGGTCT GGCCCTGAAA 
GTTGCCAGCC CACCCCCCAA CGTCAATAAT CATAGCAGCA AAGGAGACCC TCTGGAACAG 
CTGGTTCGTC ACTTTCTGAT TGAGACGGGG CCGCGAGGGG TGAAGATCAA GGGCTGTCAG 
AACGAGCCCT ACTTTGGGAG TCTTTCAGCA CTGGTGTATC AGCATTCCAT TACACCCATC 
TCTCTGCCCT GTGCCCTCCG TATCCCAGAG AAAGACCCAA TAGGTGATGT AGTGGAGGTT 
CAGCCGGTGA GTAACATGAG CACAGCCGCA GACCTCCTGA AACAGGGAGC AGCCTGTAAT 
GTGCTGTACC TGAATTCAGT GGAGACCGAG TCCCTGACGG GGCCACAGGC TATCGCTAAA 
GCTACAGGGG CCACATTGTC TCGCAGTCCT CGCCCCTCTG CCACAGTGGT CCACTTTAAG 
GTGTCTGCGC AAGGCATCAC ACTGACAGAC AGCCAGCGCA GGGTGTTCTT CAGGAGACAC 
TATCCCATCA ACAGTGTGAC TTACAGCAGC ATCGACCCAC AAGACAGGAG GTGGACTAAC 
TCAGACAGCA CGACCTCTAA AGTTTTCGGG TTTGTGGCCA AGAAGCCGGG CAGTGTGGCA 
GAGAATGTGT GCCATCTTTT CGCAGAGCTG GACCCTGAAC AGCCCGCGAC CGCCATCGTC 
AACTTCATCA ACAAAGTGAT GCTGGCACCG CAGAGGCGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tns2a ( XM_021469907.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021325582.1 Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.

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CloneID	ODa47336
Clone ID Related Accession (Same CDS sequence)	XM_009296677.2 , XM_021469907.1
Accession Version	XM_021469907.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4782bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	tensin-2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGACCTGC TCTGGGACCG CCAGCAAGGT 
CCACAGCACT ACCTGCATCG CACTTGTTCA GAGGGACCAT CTCGCCACCT GTATCCATAT 
CCATCCCAAG AGATCAATTC TCATCCACAC AGTCTGTCCC CCCGACTGCT TTGTAGACCA 
GATGAGTACA TTTCATATCC TCACCATCAT CACAATCATT CACACCATCC CAAACCCAGC 
GGACCCTACC ATGATGTCAT GATGATAGAT GGCCTAGTGA CACCCGGCTG CCCCTGTCGA 
GACTGTGCTC TACGACGAGA AGAGTTCCAC ACTTTACGTT TGGACAGAGA GGATGGAATA 
CACTGGGAGA GAGAGGCAGA GCTTCACCGG GATAATGGGT TAAGACGGAC AAGAGAGGCT 
GACATGCAGA GAGGGGCAGA GATATGGGAG CGGGAGCCAG GACTAAGGAG AGGAAGAGAG 
ATGTCTCTTC ACTGGGACAG AGACAGAGAT GCAGAACTAC AGTGGGAGAG AGAACGTGAG 
GCTGAATACT GGCACAGAAG GGCTACTGTA TCCCCATACG GGCCCCACGG TCATGAATTA 
CCTGCGTTTA ATTTTGATCC ACTCCCAGCA GGCCATCCTG CATACCCAGA ACCCTCCCGC 
TCTCACTCTC ATGCCCATCT GGATCTTAAA TACAGCAGCA GCTCCAGTGG CTACCAGACA 
CCACACCAGA CGTGCCCTTG CTCCCCCTAC CAGCCTTCAC CCTCAGAGAG TCGAGGATAT 
GCCTCGGGTT ACCAGTCTGA GTCTACGTCT CCTCTTCCGC CTCCATCTGC CTCCTGCCCC 
GGCCCGGTGC ACCATGCCAG TGGACCTACA GAAAACCAAA CTGACCCCCA TTCTCAGCAT 
TTCACCTCTG ACTCAAATAC AGATTGCCCT GGTGCAGATA GCATAGGATG GCGTAATCAT 
ATTACCCATG GATCCCTCCG GCGGATGCAT CCACATGCTC CATATTCAAC ACCCTCTGAT 
ATTTCAGGAC CTCCAACACC AGTCCACACA AGCAGCCCTT TACGAACCCA GGAAAGTCCA 
AGCCTGAGTG TCCGTGACTA TGACTCAAGA GAGGGGAACA GTGAAAGCAG TTCTGCTCAC 
TCCCTCAACA GGCCGCATCC AGCTACCATA CAGCCCAACA TGACCAATGC AGGATCACGG 
CAAACTCTAC TCTCTTCCAG TCCAGAGACT CCTGAGATTA ACCCGCTTCC TACCACACCC 
ACCACTGCCC AACATCTTGC AGCTCATTCC CTTCCTACAA CACCTCCAAC CCCACAAATT 
GCAACAGAAC ACTCACCCCC CTTAACGCCC GAGTCAGAAT CCACCCAACC ACAGGCAAAT 
TGCTCGCCTA CTGCTGCTAC AGTGCTGCCC ACAGTGGAAA TGGTGCCACA ATCAACTGAG 
ACTATAAAAC CAGCACATTC CCCACATCCC TCCTCTCCAG TCCTGGCTGA GTCCATTCCA 
GCTCACATGC AAAGCAATGG AGTACCACCA TCTCCTGAAT CAGATGCCCC TAAACCTGCT 
TCTTCAGCAC CTGCATCCCC TCAGCCACCT GCTGGTGACC ACGAGAGCTC TTCTTCCCCT 
GAACCTTCAG TGCCTGGATT TGCCACACTG GGCAGGAAGT TGATGTTGGG CTCTGAAACC 
TCACTTTCTG CAGGACACAG TATGGATGCC AGTTCTAATT CTACTCCCAC TTTCCCAGTC 
TCTCCTGCCT GCTGTACTCC ATCTCCTCCA CCAGGCTCCT ACCCAGGGTG CCCATCATCC 
TCCGTCCCAC AGCCTCCTCT CCCAGAGAAA CGCCGCCCAT CCGGCCTCCC AGGCTCACCC 
AATGGGAGGT CTGGAACACT GAGGGCATCC ATGAGTCACC AGCCCTCTGG GCAGCACCAT 
GTTACATTCT CGCCCTCAGT AGGGGAGCTG GCCGTACCCA TTGGTCAGGT TGAAGTTGGT 
GTGGAGGGTG AGATTGGCAA CCGTGTCAGT GTGAAATTTG TGCAGGACAG CTCACGATTT 
TGGTATAAAC CTGGCATCTC CAGAGAGCAA GCTATAGCAG CACTGAAGGA AAGAGAGCCA 
GGGGCCTTCC TCATCCGAGA CAGTAACTCC TTCCAGGGGG CCTACGGTCT GGCCCTGAAA 
GTTGCCAGCC CACCCCCCAA CGTCAATAAT CATAGCAGCA AAGGAGACCC TCTGGAACAG 
CTGGTTCGTC ACTTTCTGAT TGAGACGGGG CCGCGAGGGG TGAAGATCAA GGGCTGTCAG 
AACGAGCCCT ACTTTGGGAG TCTTTCAGCA CTGGTGTATC AGCATTCCAT TACACCCATC 
TCTCTGCCCT GTGCCCTCCG TATCCCAGAG AAAGACCCAA TAGGTGATGT AGTGGAGGTT 
CAGCCGGTGA GTAACATGAG CACAGCCGCA GACCTCCTGA AACAGGGAGC AGCCTGTAAT 
GTGCTGTACC TGAATTCAGT GGAGACCGAG TCCCTGACGG GGCCACAGGC TATCGCTAAA 
GCTACAGGGG CCACATTGTC TCGCAGTCCT CGCCCCTCTG CCACAGTGGT CCACTTTAAG 
GTGTCTGCGC AAGGCATCAC ACTGACAGAC AGCCAGCGCA GGGTGTTCTT CAGGAGACAC 
TATCCCATCA ACAGTGTGAC TTACAGCAGC ATCGACCCAC AAGACAGGAG GTGGACTAAC 
TCAGACAGCA CGACCTCTAA AGTTTTCGGG TTTGTGGCCA AGAAGCCGGG CAGTGTGGCA 
GAGAATGTGT GCCATCTTTT CGCAGAGCTG GACCCTGAAC AGCCCGCGAC CGCCATCGTC 
AACTTCATCA ACAAAGTGAT GCTGGCACCG CAGAGGCGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325582.1
CDS	260..5041
Translation	MGCALSGDSCGDEPDPVPLRGSLRRERAKSRGNMRLTKAGKGEPHVFKEKAFKKKRQCGVCRQSVENLGSFCRVCKTATHKKCEAKINTACIPAQPSELRRGTSSSRHIQHLGSTKSLTYTKQRNTLPRSFSVDRVMDRVMERHYDFDLTYITERIISVFFPPLLEEQRYRLNLREVAAMLKSKHQDKFLLLNLSERRHDITRLNPKVHDFGWPDMHSPPLDKICAMCKAMENWLNSDPQHVVVLHCKGNKGKTGVIIAAYMHYSKISAGADQALSTLAMRKFCEDKVASSLQPSQNRYIQYFGGLLSGSIKMNSSPLFLHQVLIPTIPNFQEDDGFFPFLKIYQSMQLVYTSGIYDLQGSASRRLIITIEPALLLKGDIMVKCYHRRVESAERDSVFRLQFHTCTVHAAQLWFGKGELDEACSDDKFPPDAIVEFVFSSGPEKIKGRVSQRNDPAVSVDYNTNDPVVRWDSYENFNQRHQDSLEDIAHTRGPVDGSLYAQVKKRRAAAGCSSLPSTNGSPARSSEERPSQLLSVSTDSGHSSVPPDRLDEPTRPAPPTQQEREELERLLGGIEGDGLRDRDRETAILDDGDSLPPERTGSLRLGRSCSCRVGYRSQGCAEPGCNGFHHLPNGHCLDRPTTTNGHTGALSPDLLWDRQQGPQHYLHRTCSEGPSRHLYPYPSQEINSHPHSLSPRLLCRPDEYISYPHHHHNHSHHPKPSGPYHDVMMIDGLVTPGCPCRDCALRREEFHTLRLDREDGIHWEREAELHRDNGLRRTREADMQRGAEIWEREPGLRRGREMSLHWDRDRDAELQWEREREAEYWHRRATVSPYGPHGHELPAFNFDPLPAGHPAYPEPSRSHSHAHLDLKYSSSSSGYQTPHQTCPCSPYQPSPSESRGYASGYQSESTSPLPPPSASCPGPVHHASGPTENQTDPHSQHFTSDSNTDCPGADSIGWRNHITHGSLRRMHPHAPYSTPSDISGPPTPVHTSSPLRTQESPSLSVRDYDSREGNSESSSAHSLNRPHPATIQPNMTNAGSRQTLLSSSPETPEINPLPTTPTTAQHLAAHSLPTTPPTPQIATEHSPPLTPESESTQPQANCSPTAATVLPTVEMVPQSTETIKPAHSPHPSSPVLAESIPAHMQSNGVPPSPESDAPKPASSAPASPQPPAGDHESSSSPEPSVPGFATLGRKLMLGSETSLSAGHSMDASSNSTPTFPVSPACCTPSPPPGSYPGCPSSSVPQPPLPEKRRPSGLPGSPNGRSGTLRASMSHQPSGQHHVTFSPSVGELAVPIGQVEVGVEGEIGNRVSVKFVQDSSRFWYKPGISREQAIAALKEREPGAFLIRDSNSFQGAYGLALKVASPPPNVNNHSSKGDPLEQLVRHFLIETGPRGVKIKGCQNEPYFGSLSALVYQHSITPISLPCALRIPEKDPIGDVVEVQPVSNMSTAADLLKQGAACNVLYLNSVETESLTGPQAIAKATGATLSRSPRPSATVVHFKVSAQGITLTDSQRRVFFRRHYPINSVTYSSIDPQDRRWTNSDSTTSKVFGFVAKKPGSVAENVCHLFAELDPEQPATAIVNFINKVMLAPQRR

Target ORF information:

RefSeq Version	XM_021469907.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tensin 2a (tns2a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469907.1

ORF Insert Sequence:

ATGGGTTGCG CCTTGAGTGG GGATAGCTGT GGGGACGAAC CAGATCCAGT ACCCTTGAGA 
GGGTCTTTAA GGAGGGAGAG AGCAAAATCC AGGGGAAACA TGAGGCTGAC CAAGGCGGGG 
AAGGGCGAGC CACACGTCTT CAAAGAAAAG GCCTTCAAAA AGAAGCGCCA ATGTGGAGTG 
TGCAGGCAGA GCGTGGAGAA CCTGGGCTCT TTTTGTCGAG TCTGCAAAAC AGCCACCCAT 
AAGAAATGCG AAGCCAAGAT CAACACGGCC TGCATCCCAG CGCAGCCCTC AGAGCTGCGA 
AGGGGAACAT CCTCCTCTAG ACACATTCAG CATCTGGGCT CAACCAAGTC TCTGACCTAC 
ACCAAACAGA GGAACACTCT CCCGAGGAGC TTCAGTGTGG ATCGGGTGAT GGACAGAGTG 
ATGGAGAGAC ACTATGATTT CGACCTCACA TACATCACAG AGAGGATCAT CTCAGTCTTC 
TTTCCTCCTC TGCTGGAGGA ACAGCGCTAC CGCCTCAACC TCAGAGAGGT GGCAGCCATG 
CTCAAGTCCA AACACCAAGA CAAGTTTCTG TTATTAAATC TGTCTGAGCG AAGGCATGAC 
ATCACCCGCT TAAACCCAAA GGTTCATGAC TTCGGCTGGC CAGATATGCA CTCTCCTCCG 
CTGGATAAGA TCTGTGCCAT GTGCAAAGCC ATGGAGAACT GGCTGAACTC TGACCCCCAG 
CATGTGGTCG TTCTGCACTG TAAGGGCAAC AAAGGAAAGA CCGGTGTAAT CATTGCTGCC 
TATATGCATT ACAGCAAGAT ATCAGCGGGG GCAGATCAGG CGCTCAGCAC GTTGGCCATG 
AGGAAGTTTT GTGAAGATAA AGTAGCGTCC TCTCTCCAGC CCTCTCAGAA CAGGTATATC 
CAGTACTTTG GAGGGCTTCT CTCAGGGTCT ATCAAAATGA ACAGCAGTCC TCTCTTCCTC 
CACCAGGTCC TCATTCCTAC CATCCCCAAC TTCCAGGAAG ATGATGGTTT CTTCCCTTTC 
CTGAAGATCT ATCAGTCCAT GCAGCTTGTC TATACTTCTG GCATATATGA TCTGCAGGGC 
TCTGCGAGCA GGCGGCTGAT CATTACTATT GAGCCTGCAT TGCTCCTGAA AGGAGACATC 
ATGGTGAAGT GTTATCACAG GCGAGTGGAG TCTGCTGAGA GAGACTCAGT GTTTCGACTG 
CAGTTTCATA CATGCACCGT TCATGCAGCT CAGCTCTGGT TCGGCAAAGG GGAACTGGAT 
GAAGCTTGTT CAGATGACAA ATTTCCCCCT GATGCCATAG TGGAGTTTGT GTTTTCATCT 
GGGCCTGAGA AAATTAAAGG GAGAGTGAGC CAAAGGAATG ATCCAGCTGT CAGTGTGGAC 
TATAACACAA ATGATCCAGT GGTGCGCTGG GACTCATATG AGAACTTCAA TCAGAGGCAT 
CAGGACAGCC TAGAGGATAT TGCTCATACA CGTGGCCCTG TAGATGGCAG CTTGTATGCA 
CAGGTTAAAA AGCGCCGCGC TGCTGCTGGC TGCTCCTCTC TGCCATCGAC CAATGGAAGC 
CCTGCAAGAA GCTCTGAGGA AAGACCAAGT CAGCTTTTGT CTGTTAGCAC TGATTCCGGA 
CACTCATCTG TTCCTCCTGA TCGACTCGAT GAACCGACTC GACCGGCCCC GCCTACTCAG 
CAAGAGAGGG AGGAGCTTGA GCGGCTATTG GGTGGGATTG AAGGCGATGG ACTGAGAGAC 
CGTGACCGAG AGACGGCTAT TTTGGATGAT GGTGATTCCT TGCCTCCTGA AAGAACTGGG 
TCTCTGAGGT TAGGCCGTTC ATGTTCTTGT CGTGTGGGTT ACCGATCTCA GGGTTGTGCT 
GAGCCTGGAT GTAATGGATT TCATCATTTA CCCAATGGCC ATTGCCTTGA TCGGCCCACC 
ACCACTAATG GACACACAGG GGCTCTGTCT CCAGACCTGC TCTGGGACCG CCAGCAAGGT 
CCACAGCACT ACCTGCATCG CACTTGTTCA GAGGGACCAT CTCGCCACCT GTATCCATAT 
CCATCCCAAG AGATCAATTC TCATCCACAC AGTCTGTCCC CCCGACTGCT TTGTAGACCA 
GATGAGTACA TTTCATATCC TCACCATCAT CACAATCATT CACACCATCC CAAACCCAGC 
GGACCCTACC ATGATGTCAT GATGATAGAT GGCCTAGTGA CACCCGGCTG CCCCTGTCGA 
GACTGTGCTC TACGACGAGA AGAGTTCCAC ACTTTACGTT TGGACAGAGA GGATGGAATA 
CACTGGGAGA GAGAGGCAGA GCTTCACCGG GATAATGGGT TAAGACGGAC AAGAGAGGCT 
GACATGCAGA GAGGGGCAGA GATATGGGAG CGGGAGCCAG GACTAAGGAG AGGAAGAGAG 
ATGTCTCTTC ACTGGGACAG AGACAGAGAT GCAGAACTAC AGTGGGAGAG AGAACGTGAG 
GCTGAATACT GGCACAGAAG GGCTACTGTA TCCCCATACG GGCCCCACGG TCATGAATTA 
CCTGCGTTTA ATTTTGATCC ACTCCCAGCA GGCCATCCTG CATACCCAGA ACCCTCCCGC 
TCTCACTCTC ATGCCCATCT GGATCTTAAA TACAGCAGCA GCTCCAGTGG CTACCAGACA 
CCACACCAGA CGTGCCCTTG CTCCCCCTAC CAGCCTTCAC CCTCAGAGAG TCGAGGATAT 
GCCTCGGGTT ACCAGTCTGA GTCTACGTCT CCTCTTCCGC CTCCATCTGC CTCCTGCCCC 
GGCCCGGTGC ACCATGCCAG TGGACCTACA GAAAACCAAA CTGACCCCCA TTCTCAGCAT 
TTCACCTCTG ACTCAAATAC AGATTGCCCT GGTGCAGATA GCATAGGATG GCGTAATCAT 
ATTACCCATG GATCCCTCCG GCGGATGCAT CCACATGCTC CATATTCAAC ACCCTCTGAT 
ATTTCAGGAC CTCCAACACC AGTCCACACA AGCAGCCCTT TACGAACCCA GGAAAGTCCA 
AGCCTGAGTG TCCGTGACTA TGACTCAAGA GAGGGGAACA GTGAAAGCAG TTCTGCTCAC 
TCCCTCAACA GGCCGCATCC AGCTACCATA CAGCCCAACA TGACCAATGC AGGATCACGG 
CAAACTCTAC TCTCTTCCAG TCCAGAGACT CCTGAGATTA ACCCGCTTCC TACCACACCC 
ACCACTGCCC AACATCTTGC AGCTCATTCC CTTCCTACAA CACCTCCAAC CCCACAAATT 
GCAACAGAAC ACTCACCCCC CTTAACGCCC GAGTCAGAAT CCACCCAACC ACAGGCAAAT 
TGCTCGCCTA CTGCTGCTAC AGTGCTGCCC ACAGTGGAAA TGGTGCCACA ATCAACTGAG 
ACTATAAAAC CAGCACATTC CCCACATCCC TCCTCTCCAG TCCTGGCTGA GTCCATTCCA 
GCTCACATGC AAAGCAATGG AGTACCACCA TCTCCTGAAT CAGATGCCCC TAAACCTGCT 
TCTTCAGCAC CTGCATCCCC TCAGCCACCT GCTGGTGACC ACGAGAGCTC TTCTTCCCCT 
GAACCTTCAG TGCCTGGATT TGCCACACTG GGCAGGAAGT TGATGTTGGG CTCTGAAACC 
TCACTTTCTG CAGGACACAG TATGGATGCC AGTTCTAATT CTACTCCCAC TTTCCCAGTC 
TCTCCTGCCT GCTGTACTCC ATCTCCTCCA CCAGGCTCCT ACCCAGGGTG CCCATCATCC 
TCCGTCCCAC AGCCTCCTCT CCCAGAGAAA CGCCGCCCAT CCGGCCTCCC AGGCTCACCC 
AATGGGAGGT CTGGAACACT GAGGGCATCC ATGAGTCACC AGCCCTCTGG GCAGCACCAT 
GTTACATTCT CGCCCTCAGT AGGGGAGCTG GCCGTACCCA TTGGTCAGGT TGAAGTTGGT 
GTGGAGGGTG AGATTGGCAA CCGTGTCAGT GTGAAATTTG TGCAGGACAG CTCACGATTT 
TGGTATAAAC CTGGCATCTC CAGAGAGCAA GCTATAGCAG CACTGAAGGA AAGAGAGCCA 
GGGGCCTTCC TCATCCGAGA CAGTAACTCC TTCCAGGGGG CCTACGGTCT GGCCCTGAAA 
GTTGCCAGCC CACCCCCCAA CGTCAATAAT CATAGCAGCA AAGGAGACCC TCTGGAACAG 
CTGGTTCGTC ACTTTCTGAT TGAGACGGGG CCGCGAGGGG TGAAGATCAA GGGCTGTCAG 
AACGAGCCCT ACTTTGGGAG TCTTTCAGCA CTGGTGTATC AGCATTCCAT TACACCCATC 
TCTCTGCCCT GTGCCCTCCG TATCCCAGAG AAAGACCCAA TAGGTGATGT AGTGGAGGTT 
CAGCCGGTGA GTAACATGAG CACAGCCGCA GACCTCCTGA AACAGGGAGC AGCCTGTAAT 
GTGCTGTACC TGAATTCAGT GGAGACCGAG TCCCTGACGG GGCCACAGGC TATCGCTAAA 
GCTACAGGGG CCACATTGTC TCGCAGTCCT CGCCCCTCTG CCACAGTGGT CCACTTTAAG 
GTGTCTGCGC AAGGCATCAC ACTGACAGAC AGCCAGCGCA GGGTGTTCTT CAGGAGACAC 
TATCCCATCA ACAGTGTGAC TTACAGCAGC ATCGACCCAC AAGACAGGAG GTGGACTAAC 
TCAGACAGCA CGACCTCTAA AGTTTTCGGG TTTGTGGCCA AGAAGCCGGG CAGTGTGGCA 
GAGAATGTGT GCCATCTTTT CGCAGAGCTG GACCCTGAAC AGCCCGCGAC CGCCATCGTC 
AACTTCATCA ACAAAGTGAT GCTGGCACCG CAGAGGCGCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	tns2a
Entrez Gene ID	799109
Full Name	tensin 2a
Synonyms	si:dkey-31e3.3,tenc1a
General protein information	Preferred Names tensin 2a Names tensin-2 tensin like C1 domain containing phosphatase a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Homo sapiens (human)	TENC1	NP_736610.2
Pan troglodytes (chimpanzee)	TENC1	XP_509087.3
	TENC1	XP_001102202.1
Danio rerio (zebrafish)	tenc1b	XP_694169.6
Rattus norvegicus (Norway rat)	Tenc1	XP_003750459.2
Danio rerio (zebrafish)	tenc1a	XP_001921043.2
Xenopus tropicalis (tropical clawed frog)	tenc1	XP_002936689.2
Bos taurus (cattle)	TENC1	XP_005206300.1
Mus musculus (house mouse)	Tenc1	NP_705761.2
Canis lupus familiaris (dog)	TENC1	XP_534792.3

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

tns2a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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見積依頼

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