gigfy2 cDNA ORF clone, Danio rerio(zebrafish)

The following gigfy2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the gigfy2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa43602 NM_001030161.2
Latest version!
Danio rerio GRB10 interacting GYF protein 2 (gigyf2), mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa56295 XM_017358653.1
Latest version!
Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa56296 XM_017358654.2
Latest version!
Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
ODa56297 XM_017358657.1
Latest version!
Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
View Old Accession Versions >>
ODa56296 XM_017358654.1 Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa43602
    Clone ID Related Accession (Same CDS sequence) NM_001030161.2
    Accession Version NM_001030161.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3990bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product GRB10-interacting GYF protein 2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX890608.15, BX890560.9 and BX677676.6. On Aug 25, 2015 this sequence version replaced NM_001030161.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC098603.1, GDQH01010399.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA
    GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT
    GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT
    ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT
    GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG
    CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA
    GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG
    GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT
    TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG
    CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA
    CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA
    GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT
    AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT
    TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG
    TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC
    CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA
    CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT
    GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT
    GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG
    CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC
    TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG
    AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG
    GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA
    CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG
    CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA
    CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG
    GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA
    CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG
    AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGCAGGAAG AGGAGCGGAG GAAGCAGGAA
    GAACTGGAAG CTCAGAGGCG GCGTGAGGAA GAGAAGAGGC TGGAGGAGGA GGCTGCGGCC
    GCTGCAGCAG CACTGCTCAG ACAACAGCAG GAGGAGCAGA AGAAGAGAGA GCAGGAGGCG
    CAGAGACAGC AGGAGCTGCA GAGACAGAGA CAGCAGCAAC AGGAGGCACT CCGACGACTC
    CAGCAGCAGC AGCAACAACA GCAGCTCGCA CAAATGAAGC TACCTTCATC CTCAAAGTGG
    GGTCAGCAGT CGACCACAGC AAACAGTCTC TCACAGTCTC AAAATGCTCT TTCGCTCGCT
    GAGATCCAGA AACTGGAGGA GGAGAGAGAA CGACAGACTC GAGAAGAGCA GAGACGTCAG
    CAGCAGGAGC TCCAGCGGGT CCAGCAGCAG CAGCCTCAGA CCAAGCTCCC TGGCTGGGGC
    AGTGTGGCTA AGCAGCCCAT GGCCACTAAA TCCCTGTTGG AGATCCAGAG AGAGGAAGCA
    CAGCAGATGA AGCAGCGGAA AGATCAGCAG CAGCAGCAAC AACAACAACA ACAACCACCT
    CCACAGCCTC AGCCTCAGCA ACATTCAACC AACACCCAGC AAAATCGCAC ACAAAACCGG
    GCGGCCATCA ATACGTCAGT GTGGGGTTCT GTCAATAATG TGAGCTCAAA CTGGATGATG
    GACAGCAGTG TATGGGGCGA CACGCAGAAC TCTAATATTG GCTTCTGGGA TGAAGCAGTA
    AAGGAGGCCG CTCCACCTCA AACCACTCGC AAGAGCCACA CTCCGAAAAA CAAGGGCAAC
    GCTAACCTCA GTAATAGCAG CAGCGGTAAA GCCAGTAAAA AGGTGGAAGA GGAGGAGAAG
    CTACTGAAAC TGTTCCAGGG AGCCAATAAG AACCAGGACG GCTTTATGCA GTGGTGTGAA
    CAGACCCTGC ACACTCTCAA CACAGCCAAT AACCTCGATG TTCCCACTTT TGCATCGTTC
    CTGAAGGAAG TGGACTCTCC GTATGAGGTG CACGATTATG TACGAGCTTA TCTGGGTGAC
    ACTCCACAGG CCAAGGACTT CGCCAAGCAG TTCCTGGAGC GCCGTGCCAA ACAGAACGAC
    AACCAGCAGA AACCTCAGCA GGGCCAGCAA CAAAAACAGC AGGAGTCGGT GTGGGGAATA
    AGAGAAGTGC CACAGTCAGT CCTGCTTCAA CAACAACAAC AACAACAATT ACAGCAGCAG
    CAACAGCAGC GGTTTGAAAC GGTCACTTCC GGCAAGAAGA AGAAGAAACA GAAGATGGTA
    CGAGCCGACC CAAGCCTTCT AGGTTTTTCT GTCAATGCCT CCTCTGAGAG ACTCAATATG
    GGCGAGATCG AGACGGTTGA AGACTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001025332.2
    CDS37..4026
    Translation

    Target ORF information:

    RefSeq Version NM_001030161.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GRB10 interacting GYF protein 2 (gigyf2), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001030161.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA
    GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT
    GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT
    ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT
    GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG
    CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA
    GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG
    GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT
    TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG
    CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA
    CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA
    GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT
    AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT
    TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG
    TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC
    CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA
    CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT
    GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT
    GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG
    CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC
    TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG
    AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG
    GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA
    CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG
    CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA
    CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG
    GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA
    CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG
    AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGCAGGAAG AGGAGCGGAG GAAGCAGGAA
    GAACTGGAAG CTCAGAGGCG GCGTGAGGAA GAGAAGAGGC TGGAGGAGGA GGCTGCGGCC
    GCTGCAGCAG CACTGCTCAG ACAACAGCAG GAGGAGCAGA AGAAGAGAGA GCAGGAGGCG
    CAGAGACAGC AGGAGCTGCA GAGACAGAGA CAGCAGCAAC AGGAGGCACT CCGACGACTC
    CAGCAGCAGC AGCAACAACA GCAGCTCGCA CAAATGAAGC TACCTTCATC CTCAAAGTGG
    GGTCAGCAGT CGACCACAGC AAACAGTCTC TCACAGTCTC AAAATGCTCT TTCGCTCGCT
    GAGATCCAGA AACTGGAGGA GGAGAGAGAA CGACAGACTC GAGAAGAGCA GAGACGTCAG
    CAGCAGGAGC TCCAGCGGGT CCAGCAGCAG CAGCCTCAGA CCAAGCTCCC TGGCTGGGGC
    AGTGTGGCTA AGCAGCCCAT GGCCACTAAA TCCCTGTTGG AGATCCAGAG AGAGGAAGCA
    CAGCAGATGA AGCAGCGGAA AGATCAGCAG CAGCAGCAAC AACAACAACA ACAACCACCT
    CCACAGCCTC AGCCTCAGCA ACATTCAACC AACACCCAGC AAAATCGCAC ACAAAACCGG
    GCGGCCATCA ATACGTCAGT GTGGGGTTCT GTCAATAATG TGAGCTCAAA CTGGATGATG
    GACAGCAGTG TATGGGGCGA CACGCAGAAC TCTAATATTG GCTTCTGGGA TGAAGCAGTA
    AAGGAGGCCG CTCCACCTCA AACCACTCGC AAGAGCCACA CTCCGAAAAA CAAGGGCAAC
    GCTAACCTCA GTAATAGCAG CAGCGGTAAA GCCAGTAAAA AGGTGGAAGA GGAGGAGAAG
    CTACTGAAAC TGTTCCAGGG AGCCAATAAG AACCAGGACG GCTTTATGCA GTGGTGTGAA
    CAGACCCTGC ACACTCTCAA CACAGCCAAT AACCTCGATG TTCCCACTTT TGCATCGTTC
    CTGAAGGAAG TGGACTCTCC GTATGAGGTG CACGATTATG TACGAGCTTA TCTGGGTGAC
    ACTCCACAGG CCAAGGACTT CGCCAAGCAG TTCCTGGAGC GCCGTGCCAA ACAGAACGAC
    AACCAGCAGA AACCTCAGCA GGGCCAGCAA CAAAAACAGC AGGAGTCGGT GTGGGGAATA
    AGAGAAGTGC CACAGTCAGT CCTGCTTCAA CAACAACAAC AACAACAATT ACAGCAGCAG
    CAACAGCAGC GGTTTGAAAC GGTCACTTCC GGCAAGAAGA AGAAGAAACA GAAGATGGTA
    CGAGCCGACC CAAGCCTTCT AGGTTTTTCT GTCAATGCCT CCTCTGAGAG ACTCAATATG
    GGCGAGATCG AGACGGTTGA AGACTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56295
    Clone ID Related Accession (Same CDS sequence) XM_017358653.1
    Accession Version XM_017358653.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3987bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA
    GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT
    GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT
    ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT
    GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG
    CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA
    GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG
    GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT
    TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG
    CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA
    CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA
    GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT
    AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT
    TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG
    TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC
    CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA
    CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT
    GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT
    GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG
    CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC
    TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG
    AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG
    GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA
    CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG
    CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA
    CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG
    GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA
    CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG
    AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGGAAGAGG AGCGGAGGAA GCAGGAAGAA
    CTGGAAGCTC AGAGGCGGCG TGAGGAAGAG AAGAGGCTGG AGGAGGAGGC TGCGGCCGCT
    GCAGCAGCAC TGCTCAGACA ACAGCAGGAG GAGCAGAAGA AGAGAGAGCA GGAGGCGCAG
    AGACAGCAGG AGCTGCAGAG ACAGAGACAG CAGCAACAGG AGGCACTCCG ACGACTCCAG
    CAGCAGCAGC AACAACAGCA GCTCGCACAA ATGAAGCTAC CTTCATCCTC AAAGTGGGGT
    CAGCAGTCGA CCACAGCAAA CAGTCTCTCA CAGTCTCAAA ATGCTCTTTC GCTCGCTGAG
    ATCCAGAAAC TGGAGGAGGA GAGAGAACGA CAGACTCGAG AAGAGCAGAG ACGTCAGCAG
    CAGGAGCTCC AGCGGGTCCA GCAGCAGCAG CCTCAGACCA AGCTCCCTGG CTGGGGCAGT
    GTGGCTAAGC AGCCCATGGC CACTAAATCC CTGTTGGAGA TCCAGAGAGA GGAAGCACAG
    CAGATGAAGC AGCGGAAAGA TCAGCAGCAG CAGCAACAAC AACAACAACA ACCACCTCCA
    CAGCCTCAGC CTCAGCAACA TTCAACCAAC ACCCAGCAAA ATCGCACACA AAACCGGGCG
    GCCATCAATA CGTCAGTGTG GGGTTCTGTC AATAATGTGA GCTCAAACTG GATGATGGAC
    AGCAGTGTAT GGGGCGACAC GCAGAACTCT AATATTGGCT TCTGGGATGA AGCAGTAAAG
    GAGGCCGCTC CACCTCAAAC CACTCGCAAG AGCCACACTC CGAAAAACAA GGGCAACGCT
    AACCTCAGTA ATAGCAGCAG CGGTAAAGCC AGTAAAAAGG TGGAAGAGGA GGAGAAGCTA
    CTGAAACTGT TCCAGGGAGC CAATAAGAAC CAGGACGGCT TTATGCAGTG GTGTGAACAG
    ACCCTGCACA CTCTCAACAC AGCCAATAAC CTCGATGTTC CCACTTTTGC ATCGTTCCTG
    AAGGAAGTGG ACTCTCCGTA TGAGGTGCAC GATTATGTAC GAGCTTATCT GGGTGACACT
    CCACAGGCCA AGGACTTCGC CAAGCAGTTC CTGGAGCGCC GTGCCAAACA GAACGACAAC
    CAGCAGAAAC CTCAGCAGGG CCAGCAACAA AAACAGCAGG AGTCGGTGTG GGGAATAAGA
    GAAGTGCCAC AGTCAGTCCT GCTTCAACAA CAACAACAAC AACAATTACA GCAGCAGCAA
    CAGCAGCGGT TTGAAACGGT CACTTCCGGC AAGAAGAAGA AGAAACAGAA GATGGTACGA
    GCCGACCCAA GCCTTCTAGG TTTTTCTGTC AATGCCTCCT CTGAGAGACT CAATATGGGC
    GAGATCGAGA CGGTTGAAGA CTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214142.1
    CDS102..4088
    Translation

    Target ORF information:

    RefSeq Version XM_017358653.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358653.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGGTGAGGGC GCTGGAAAAA
    GCTCCTAAGG AGCCAATCCT GGAAGAGGCG GAGCTGGACT TCCGGCCATT GGAGGAGAAT
    GACGAATATG CAGAAAAGGA TGACAGTGAA ACTGAACAAA CCAAAGATAC TGACACAAAT
    ACTAGGCACG AAAGTGATCG AAATGAAGAA AACTGTAAAT CAGAAGAGCC GTCGCCAGTT
    GCGGTACCAT TTTCAGCAGT AGTTACGCCT CCCAAAGCCA CCACCCCTGC ACCCATTCAG
    CCTGTACACC TAGAAAAAGC TGAAGATAAG GAGAGACCGT CAGAGAGAAC AACATTACCA
    GAAATCAGAC ATGAACTGAG TAAAGCACCA CTACACACAG CCCTATCTAA CAGCATTGTG
    GAAGCCATTT CCATACCGCA TGTCGCAAAC AAACTCCCAG ATCTTCCGGT CCCAGCGCCT
    TCAGTTCTGC CTGTCAAGTC AGTGCCCCCT CAATCCCAAC AGGTCAAGCC TATAGAGATG
    CCAGTGTCAG TTCCTCCTGC TCTGCTACGC TCCACAGGCA GTGTGGGTCC TATCAGTCGA
    CCCTCTGCCC TGCCATCGGA CTTGGATGAG GATGAGGGAC TCAAGCACTT TGAGCAGGAA
    GCTGAGAAAA TGGTGGCGTA CCTCCAGGAT GGTGGCGTAG ATGATGAACG TTTGGCCTCT
    AAAATTGGAC CCAAACCATC AGCTCTTCCA ATCACAAATG AAGCTGCATT CAAATGGTTT
    TACAAAGATC CGCAGGGGGA AATCCAAGGA CCATTTAATA ATCAGGAGAT GTCTGAGTGG
    TTTCAGGCGG GCTATTTCAC CATGACCTTG CAGGTAAAGC GAGGATGCGA CGAGATGTTC
    CAGCCTCTGG GCGAGATGAT TAAGTTGTGG GGACGAGTGC CCTTTACACC GGGCCCGACA
    CTACCTCCCA TACTGGGAGA TGCAGATCAA GAAAGGATGA AGAGACAACA GGAGATTAAT
    GCTCTAAATA TGTATCAGCT ACAGCAGATG CAATATCAGT ATTTACTCAG GCAACAATAT
    GCTCTGCAGC AGAAAGTGTT AAACTCTGCT CCTCCTCCTC CTCCACCACC ACAGCAGCAG
    CTCAATCTTC TTCTCCATCA GGCGCTGAAG ATCAGAACTC CTGAGCCGCA GCAGAGCCTC
    TTACCTCCCG TCACACGCTC CATGTCGGTT CCAGACTCGG GTTCTGTTTG GGAAATGCAG
    AACCCGTCCA CTCAGGCATC CTGTTCTCCA AACATGCAGC CCGCCGCTCC CAGCACTTGG
    GAAGGGAGTA GCGTATGGGA TCTGCCACTA GACAATATGC CTCAAGCGTC TTCCATTGAA
    CAGCTGCAGC TGGAGAAAGC CAAAGCTTTG AAGCTGGAGA TGGAGAGACG GGAGGCAGAG
    CTTCGGGCCA AAAGGGAAGA AGAGGAGAGG AAGCGTTTGG AAGAGGCTCT GCGTGCGAGA
    CAAGAGGAAG AGAGGAAGCG TTTAGAGGAA GAAGAGCTGG CACGACGCAA GCAGGAGGAG
    GCTCTGAAGA GACAGCGGGA GCAGGAGGAG GCGCAGCGGA GGAAAAAGGA AGAGGAGGAA
    CGGTTAGCTC AGGAGGAGGC TCTCCGGCGA CTGGAGGAGC GGAGGAGAGA AGAAGAGGAG
    AGGAGACAAA GGGAAGAATT CCTCCGCAAA CAGGAAGAGG AGCGGAGGAA GCAGGAAGAA
    CTGGAAGCTC AGAGGCGGCG TGAGGAAGAG AAGAGGCTGG AGGAGGAGGC TGCGGCCGCT
    GCAGCAGCAC TGCTCAGACA ACAGCAGGAG GAGCAGAAGA AGAGAGAGCA GGAGGCGCAG
    AGACAGCAGG AGCTGCAGAG ACAGAGACAG CAGCAACAGG AGGCACTCCG ACGACTCCAG
    CAGCAGCAGC AACAACAGCA GCTCGCACAA ATGAAGCTAC CTTCATCCTC AAAGTGGGGT
    CAGCAGTCGA CCACAGCAAA CAGTCTCTCA CAGTCTCAAA ATGCTCTTTC GCTCGCTGAG
    ATCCAGAAAC TGGAGGAGGA GAGAGAACGA CAGACTCGAG AAGAGCAGAG ACGTCAGCAG
    CAGGAGCTCC AGCGGGTCCA GCAGCAGCAG CCTCAGACCA AGCTCCCTGG CTGGGGCAGT
    GTGGCTAAGC AGCCCATGGC CACTAAATCC CTGTTGGAGA TCCAGAGAGA GGAAGCACAG
    CAGATGAAGC AGCGGAAAGA TCAGCAGCAG CAGCAACAAC AACAACAACA ACCACCTCCA
    CAGCCTCAGC CTCAGCAACA TTCAACCAAC ACCCAGCAAA ATCGCACACA AAACCGGGCG
    GCCATCAATA CGTCAGTGTG GGGTTCTGTC AATAATGTGA GCTCAAACTG GATGATGGAC
    AGCAGTGTAT GGGGCGACAC GCAGAACTCT AATATTGGCT TCTGGGATGA AGCAGTAAAG
    GAGGCCGCTC CACCTCAAAC CACTCGCAAG AGCCACACTC CGAAAAACAA GGGCAACGCT
    AACCTCAGTA ATAGCAGCAG CGGTAAAGCC AGTAAAAAGG TGGAAGAGGA GGAGAAGCTA
    CTGAAACTGT TCCAGGGAGC CAATAAGAAC CAGGACGGCT TTATGCAGTG GTGTGAACAG
    ACCCTGCACA CTCTCAACAC AGCCAATAAC CTCGATGTTC CCACTTTTGC ATCGTTCCTG
    AAGGAAGTGG ACTCTCCGTA TGAGGTGCAC GATTATGTAC GAGCTTATCT GGGTGACACT
    CCACAGGCCA AGGACTTCGC CAAGCAGTTC CTGGAGCGCC GTGCCAAACA GAACGACAAC
    CAGCAGAAAC CTCAGCAGGG CCAGCAACAA AAACAGCAGG AGTCGGTGTG GGGAATAAGA
    GAAGTGCCAC AGTCAGTCCT GCTTCAACAA CAACAACAAC AACAATTACA GCAGCAGCAA
    CAGCAGCGGT TTGAAACGGT CACTTCCGGC AAGAAGAAGA AGAAACAGAA GATGGTACGA
    GCCGACCCAA GCCTTCTAGG TTTTTCTGTC AATGCCTCCT CTGAGAGACT CAATATGGGC
    GAGATCGAGA CGGTTGAAGA CTTTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56296
    Clone ID Related Accession (Same CDS sequence) XM_017358654.1 , XM_017358654.2
    Accession Version XM_017358654.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product PERQ amino acid-rich with GYF domain-containing protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA
    ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA
    AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT
    GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA
    GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA
    AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA
    CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA
    CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC
    AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT
    CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA
    TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG
    GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA
    CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG
    GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT
    TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG
    ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG
    GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT
    CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA
    GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC
    CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA
    CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG
    GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA
    TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG
    AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG
    GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG
    AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG
    CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG
    GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA
    GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG
    AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG
    CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG
    CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA
    CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC
    ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG
    GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG
    CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG
    CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG
    CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT
    CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG
    TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG
    GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA
    CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT
    AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC
    CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT
    CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC
    TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG
    GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT
    CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG
    TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT
    GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC
    CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG
    GTTGAAGACT TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214143.1
    CDS102..4076
    Translation

    Target ORF information:

    RefSeq Version XM_017358654.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358654.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA
    ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA
    AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT
    GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA
    GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA
    AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA
    CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA
    CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC
    AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT
    CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA
    TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG
    GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA
    CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG
    GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT
    TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG
    ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG
    GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT
    CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA
    GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC
    CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA
    CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG
    GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA
    TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG
    AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG
    GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG
    AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG
    CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG
    GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA
    GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG
    AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG
    CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG
    CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA
    CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC
    ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG
    GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG
    CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG
    CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG
    CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT
    CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG
    TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG
    GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA
    CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT
    AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC
    CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT
    CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC
    TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG
    GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT
    CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG
    TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT
    GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC
    CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG
    GTTGAAGACT TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56296
    Clone ID Related Accession (Same CDS sequence) XM_017358654.1 , XM_017358654.2
    Accession Version XM_017358654.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3975bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product GRB10-interacting GYF protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358654.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA
    ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA
    AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT
    GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA
    GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA
    AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA
    CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA
    CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC
    AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT
    CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA
    TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG
    GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA
    CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG
    GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT
    TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG
    ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG
    GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT
    CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA
    GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC
    CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA
    CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG
    GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA
    TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG
    AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG
    GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG
    AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG
    CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG
    GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA
    GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG
    AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG
    CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG
    CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA
    CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC
    ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG
    GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG
    CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG
    CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG
    CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT
    CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG
    TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG
    GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA
    CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT
    AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC
    CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT
    CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC
    TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG
    GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT
    CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG
    TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT
    GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC
    CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG
    GTTGAAGACT TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214143.1
    CDS143..4117
    Translation

    Target ORF information:

    RefSeq Version XM_017358654.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358654.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA
    ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA
    AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT
    GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA
    GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA
    AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA
    CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA
    CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC
    AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT
    CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA
    TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG
    GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA
    CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG
    GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT
    TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG
    ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG
    GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT
    CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA
    GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC
    CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA
    CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG
    GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA
    TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG
    AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG
    GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG
    AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG
    CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG
    GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA
    GAATTCCTCC GCAAACAGCA GGAAGAGGAG CGGAGGAAGC AGGAAGAACT GGAAGCTCAG
    AGGCGGCGTG AGGAAGAGAA GAGGCTGGAG GAGGAGGCTG CGGCCGCTGC AGCAGCACTG
    CTCAGACAAC AGCAGGAGGA GCAGAAGAAG AGAGAGCAGG AGGCGCAGAG ACAGCAGGAG
    CTGCAGAGAC AGAGACAGCA GCAACAGGAG GCACTCCGAC GACTCCAGCA GCAGCAGCAA
    CAACAGCAGC TCGCACAAAT GAAGCTACCT TCATCCTCAA AGTGGGGTCA GCAGTCGACC
    ACAGCAAACA GTCTCTCACA GTCTCAAAAT GCTCTTTCGC TCGCTGAGAT CCAGAAACTG
    GAGGAGGAGA GAGAACGACA GACTCGAGAA GAGCAGAGAC GTCAGCAGCA GGAGCTCCAG
    CGGGTCCAGC AGCAGCAGCC TCAGACCAAG CTCCCTGGCT GGGGCAGTGT GGCTAAGCAG
    CCCATGGCCA CTAAATCCCT GTTGGAGATC CAGAGAGAGG AAGCACAGCA GATGAAGCAG
    CGGAAAGATC AGCAGCAGCA GCAACAACAA CAACAACAAC CACCTCCACA GCCTCAGCCT
    CAGCAACATT CAACCAACAC CCAGCAAAAT CGCACACAAA ACCGGGCGGC CATCAATACG
    TCAGTGTGGG GTTCTGTCAA TAATGTGAGC TCAAACTGGA TGATGGACAG CAGTGTATGG
    GGCGACACGC AGAACTCTAA TATTGGCTTC TGGGATGAAG CAGTAAAGGA GGCCGCTCCA
    CCTCAAACCA CTCGCAAGAG CCACACTCCG AAAAACAAGG GCAACGCTAA CCTCAGTAAT
    AGCAGCAGCG GTAAAGCCAG TAAAAAGGTG GAAGAGGAGG AGAAGCTACT GAAACTGTTC
    CAGGGAGCCA ATAAGAACCA GGACGGCTTT ATGCAGTGGT GTGAACAGAC CCTGCACACT
    CTCAACACAG CCAATAACCT CGATGTTCCC ACTTTTGCAT CGTTCCTGAA GGAAGTGGAC
    TCTCCGTATG AGGTGCACGA TTATGTACGA GCTTATCTGG GTGACACTCC ACAGGCCAAG
    GACTTCGCCA AGCAGTTCCT GGAGCGCCGT GCCAAACAGA ACGACAACCA GCAGAAACCT
    CAGCAGGGCC AGCAACAAAA ACAGCAGGAG TCGGTGTGGG GAATAAGAGA AGTGCCACAG
    TCAGTCCTGC TTCAACAACA ACAACAACAA CAATTACAGC AGCAGCAACA GCAGCGGTTT
    GAAACGGTCA CTTCCGGCAA GAAGAAGAAG AAACAGAAGA TGGTACGAGC CGACCCAAGC
    CTTCTAGGTT TTTCTGTCAA TGCCTCCTCT GAGAGACTCA ATATGGGCGA GATCGAGACG
    GTTGAAGACT TTTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa56297
    Clone ID Related Accession (Same CDS sequence) XM_017358657.1
    Accession Version XM_017358657.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product PERQ amino acid-rich with GYF domain-containing protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007113.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA
    ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA
    AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT
    GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA
    GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA
    AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA
    CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA
    CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC
    AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT
    CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA
    TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG
    GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA
    CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG
    GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT
    TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG
    ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG
    GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT
    CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA
    GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC
    CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA
    CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG
    GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA
    TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG
    AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG
    GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG
    AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG
    CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG
    GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA
    GAATTCCTCC GCAAACAGGA AGAGGAGCGG AGGAAGCAGG AAGAACTGGA AGCTCAGAGG
    CGGCGTGAGG AAGAGAAGAG GCTGGAGGAG GAGGCTGCGG CCGCTGCAGC AGCACTGCTC
    AGACAACAGC AGGAGGAGCA GAAGAAGAGA GAGCAGGAGG CGCAGAGACA GCAGGAGCTG
    CAGAGACAGA GACAGCAGCA ACAGGAGGCA CTCCGACGAC TCCAGCAGCA GCAGCAACAA
    CAGCAGCTCG CACAAATGAA GCTACCTTCA TCCTCAAAGT GGGGTCAGCA GTCGACCACA
    GCAAACAGTC TCTCACAGTC TCAAAATGCT CTTTCGCTCG CTGAGATCCA GAAACTGGAG
    GAGGAGAGAG AACGACAGAC TCGAGAAGAG CAGAGACGTC AGCAGCAGGA GCTCCAGCGG
    GTCCAGCAGC AGCAGCCTCA GACCAAGCTC CCTGGCTGGG GCAGTGTGGC TAAGCAGCCC
    ATGGCCACTA AATCCCTGTT GGAGATCCAG AGAGAGGAAG CACAGCAGAT GAAGCAGCGG
    AAAGATCAGC AGCAGCAGCA ACAACAACAA CAACAACCAC CTCCACAGCC TCAGCCTCAG
    CAACATTCAA CCAACACCCA GCAAAATCGC ACACAAAACC GGGCGGCCAT CAATACGTCA
    GTGTGGGGTT CTGTCAATAA TGTGAGCTCA AACTGGATGA TGGACAGCAG TGTATGGGGC
    GACACGCAGA ACTCTAATAT TGGCTTCTGG GATGAAGCAG TAAAGGAGGC CGCTCCACCT
    CAAACCACTC GCAAGAGCCA CACTCCGAAA AACAAGGGCA ACGCTAACCT CAGTAATAGC
    AGCAGCGGTA AAGCCAGTAA AAAGGTGGAA GAGGAGGAGA AGCTACTGAA ACTGTTCCAG
    GGAGCCAATA AGAACCAGGA CGGCTTTATG CAGTGGTGTG AACAGACCCT GCACACTCTC
    AACACAGCCA ATAACCTCGA TGTTCCCACT TTTGCATCGT TCCTGAAGGA AGTGGACTCT
    CCGTATGAGG TGCACGATTA TGTACGAGCT TATCTGGGTG ACACTCCACA GGCCAAGGAC
    TTCGCCAAGC AGTTCCTGGA GCGCCGTGCC AAACAGAACG ACAACCAGCA GAAACCTCAG
    CAGGGCCAGC AACAAAAACA GCAGGAGTCG GTGTGGGGAA TAAGAGAAGT GCCACAGTCA
    GTCCTGCTTC AACAACAACA ACAACAACAA TTACAGCAGC AGCAACAGCA GCGGTTTGAA
    ACGGTCACTT CCGGCAAGAA GAAGAAGAAA CAGAAGATGG TACGAGCCGA CCCAAGCCTT
    CTAGGTTTTT CTGTCAATGC CTCCTCTGAG AGACTCAATA TGGGCGAGAT CGAGACGGTT
    GAAGACTTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214146.1
    CDS102..4073
    Translation

    Target ORF information:

    RefSeq Version XM_017358657.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio GRB10 interacting GYF protein 2 (gigfy2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358657.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCGGAAA CCCAGACACT CAACTTTGGG CCAGAATGGC TCCGTGCTCT GGCTGGTGGG 
    GTGGGCAGCA GCATTGTGGC CTCCCCTCCG CTCTCGCCTG CATTGCCAAA GTATAAACTT
    GCAGACTATC GCTACGGGAG AGAAGAGATG CTAGCACTTT ATGTAAAGGA TAACATGATT
    CCGGTAGATC TTCATGATAA GGAATTCCTA CCTATTTTAC AAGAGGAGCC CCTGCCACCT
    TTGGCATTAG TCCCTTTTAC AGAGGAGGAA CAGAGAAATT TCTCCATGTC TGTAAACAGT
    GCAGCAGTTC TCCGTCTGAC AGGAAGGGGG GGAGGGACGG TGGCAGGGGC ACCTAGAGGC
    CGAAGTTCTT CACGAGGGCG AGGGAGAGGC AGAGGAGATG GTTTTTACCA AAGAAGTTTT
    GATGATGTGG AAGGAGGTTT CGGTCGTGGA GGGAGAGAGA TGCATCGCTC CCAGAGTTGG
    GAGGAGAGGG GAGACAGAAG GTTTGAAAAG CCAGGTCGAA AAGATCCAGA TGGAGCACCA
    GCCCATTTTC CACTCAATCA CATAAGGGCA AACTATGAGG ACCCTGCTGC AGTGAATGCC
    CGAAAGTTCA TTCATGCAGA GTGTGACAAC TGGCGCACCA CTCGAGATGA GCTGAATGGA
    GAGGAGGATG ATAACGGATG GAGGCTTGCA GGCGCTCGTC GGGAAAATGA GCGATGGTGT
    CCTCCAAGTC CAGATGGTCC ACGATCAGCA GGGTGGCGGG AACACCCAGA TCAACGTCGA
    CGCTTTGGTT TTGGGGATGA GGAACGCAGT GGTTACCGTA GGCCTCGGTC GGGCAGCGGG
    AGCGCAGAGG AGGAGAGGGA CAGTTTACCA GAATGGTGTC TGGAAGATGC AGAGGAGGAG
    ACAGGGACGT TTGACTCCTC AGGGGCCTTC CTCTCACTTA AGAAAGCTCC TAAGGAGCCA
    ATCCTGGAAG AGGCGGAGCT GGACTTCCGG CCATTGGAGG AGAATGACGA ATATGCAGAA
    AAGGATGACA GTGAAACTGA ACAAACCAAA GATACTGACA CAAATACTAG GCACGAAAGT
    GATCGAAATG AAGAAAACTG TAAATCAGAA GAGCCGTCGC CAGTTGCGGT ACCATTTTCA
    GCAGTAGTTA CGCCTCCCAA AGCCACCACC CCTGCACCCA TTCAGCCTGT ACACCTAGAA
    AAAGCTGAAG ATAAGGAGAG ACCGTCAGAG AGAACAACAT TACCAGAAAT CAGACATGAA
    CTGAGTAAAG CACCACTACA CACAGCCCTA TCTAACAGCA TTGTGGAAGC CATTTCCATA
    CCGCATGTCG CAAACAAACT CCCAGATCTT CCGGTCCCAG CGCCTTCAGT TCTGCCTGTC
    AAGTCAGTGC CCCCTCAATC CCAACAGGTC AAGCCTATAG AGATGCCAGT GTCAGTTCCT
    CCTGCTCTGC TACGCTCCAC AGGCAGTGTG GGTCCTATCA GTCGACCCTC TGCCCTGCCA
    TCGGACTTGG ATGAGGATGA GGGACTCAAG CACTTTGAGC AGGAAGCTGA GAAAATGGTG
    GCGTACCTCC AGGATGGTGG CGTAGATGAT GAACGTTTGG CCTCTAAAAT TGGACCCAAA
    CCATCAGCTC TTCCAATCAC AAATGAAGCT GCATTCAAAT GGTTTTACAA AGATCCGCAG
    GGGGAAATCC AAGGACCATT TAATAATCAG GAGATGTCTG AGTGGTTTCA GGCGGGCTAT
    TTCACCATGA CCTTGCAGGT AAAGCGAGGA TGCGACGAGA TGTTCCAGCC TCTGGGCGAG
    ATGATTAAGT TGTGGGGACG AGTGCCCTTT ACACCGGGCC CGACACTACC TCCCATACTG
    GGAGATGCAG ATCAAGAAAG GATGAAGAGA CAACAGGAGA TTAATGCTCT AAATATGTAT
    CAGCTACAGC AGATGCAATA TCAGTATTTA CTCAGGCAAC AATATGCTCT GCAGCAGAAA
    GTGTTAAACT CTGCTCCTCC TCCTCCTCCA CCACCACAGC AGCAGCTCAA TCTTCTTCTC
    CATCAGGCGC TGAAGATCAG AACTCCTGAG CCGCAGCAGA GCCTCTTACC TCCCGTCACA
    CGCTCCATGT CGGTTCCAGA CTCGGGTTCT GTTTGGGAAA TGCAGAACCC GTCCACTCAG
    GCATCCTGTT CTCCAAACAT GCAGCCCGCC GCTCCCAGCA CTTGGGAAGG GAGTAGCGTA
    TGGGATCTGC CACTAGACAA TATGCCTCAA GCGTCTTCCA TTGAACAGCT GCAGCTGGAG
    AAAGCCAAAG CTTTGAAGCT GGAGATGGAG AGACGGGAGG CAGAGCTTCG GGCCAAAAGG
    GAAGAAGAGG AGAGGAAGCG TTTGGAAGAG GCTCTGCGTG CGAGACAAGA GGAAGAGAGG
    AAGCGTTTAG AGGAAGAAGA GCTGGCACGA CGCAAGCAGG AGGAGGCTCT GAAGAGACAG
    CGGGAGCAGG AGGAGGCGCA GCGGAGGAAA AAGGAAGAGG AGGAACGGTT AGCTCAGGAG
    GAGGCTCTCC GGCGACTGGA GGAGCGGAGG AGAGAAGAAG AGGAGAGGAG ACAAAGGGAA
    GAATTCCTCC GCAAACAGGA AGAGGAGCGG AGGAAGCAGG AAGAACTGGA AGCTCAGAGG
    CGGCGTGAGG AAGAGAAGAG GCTGGAGGAG GAGGCTGCGG CCGCTGCAGC AGCACTGCTC
    AGACAACAGC AGGAGGAGCA GAAGAAGAGA GAGCAGGAGG CGCAGAGACA GCAGGAGCTG
    CAGAGACAGA GACAGCAGCA ACAGGAGGCA CTCCGACGAC TCCAGCAGCA GCAGCAACAA
    CAGCAGCTCG CACAAATGAA GCTACCTTCA TCCTCAAAGT GGGGTCAGCA GTCGACCACA
    GCAAACAGTC TCTCACAGTC TCAAAATGCT CTTTCGCTCG CTGAGATCCA GAAACTGGAG
    GAGGAGAGAG AACGACAGAC TCGAGAAGAG CAGAGACGTC AGCAGCAGGA GCTCCAGCGG
    GTCCAGCAGC AGCAGCCTCA GACCAAGCTC CCTGGCTGGG GCAGTGTGGC TAAGCAGCCC
    ATGGCCACTA AATCCCTGTT GGAGATCCAG AGAGAGGAAG CACAGCAGAT GAAGCAGCGG
    AAAGATCAGC AGCAGCAGCA ACAACAACAA CAACAACCAC CTCCACAGCC TCAGCCTCAG
    CAACATTCAA CCAACACCCA GCAAAATCGC ACACAAAACC GGGCGGCCAT CAATACGTCA
    GTGTGGGGTT CTGTCAATAA TGTGAGCTCA AACTGGATGA TGGACAGCAG TGTATGGGGC
    GACACGCAGA ACTCTAATAT TGGCTTCTGG GATGAAGCAG TAAAGGAGGC CGCTCCACCT
    CAAACCACTC GCAAGAGCCA CACTCCGAAA AACAAGGGCA ACGCTAACCT CAGTAATAGC
    AGCAGCGGTA AAGCCAGTAA AAAGGTGGAA GAGGAGGAGA AGCTACTGAA ACTGTTCCAG
    GGAGCCAATA AGAACCAGGA CGGCTTTATG CAGTGGTGTG AACAGACCCT GCACACTCTC
    AACACAGCCA ATAACCTCGA TGTTCCCACT TTTGCATCGT TCCTGAAGGA AGTGGACTCT
    CCGTATGAGG TGCACGATTA TGTACGAGCT TATCTGGGTG ACACTCCACA GGCCAAGGAC
    TTCGCCAAGC AGTTCCTGGA GCGCCGTGCC AAACAGAACG ACAACCAGCA GAAACCTCAG
    CAGGGCCAGC AACAAAAACA GCAGGAGTCG GTGTGGGGAA TAAGAGAAGT GCCACAGTCA
    GTCCTGCTTC AACAACAACA ACAACAACAA TTACAGCAGC AGCAACAGCA GCGGTTTGAA
    ACGGTCACTT CCGGCAAGAA GAAGAAGAAA CAGAAGATGG TACGAGCCGA CCCAAGCCTT
    CTAGGTTTTT CTGTCAATGC CTCCTCTGAG AGACTCAATA TGGGCGAGAT CGAGACGGTT
    GAAGACTTTT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.