GIGYF2 cDNA ORF clone, Canis lupus familiaris(dog)

The following GIGYF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIGYF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OCe25827 XM_005635856.3
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe25828 XM_856835.5
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe25829 XM_005635857.2
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe25830 XM_005635858.2
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe297280 XM_022410015.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297280 XM_022410016.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297281 XM_022410017.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297282 XM_022410018.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297283 XM_022410019.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297284 XM_022410020.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297285 XM_022410021.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297286 XM_022410022.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297287 XM_022410023.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe297288 XM_022410024.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X12, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe297289 XM_022410025.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X13, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
OCe297290 XM_022410026.1
Latest version!
Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X14, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $629.30
$899.00
View Old Accession Versions >>
OCe25827 XM_005635856.2 Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
OCe25828 XM_856835.4 Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $713.30
$1019.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone 30% OFF
2-4 Clone 40% OFF
5 or more Clone 50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OCe25827
    Clone ID Related Accession (Same CDS sequence) XM_005635856.2 , XM_005635856.3
    Accession Version XM_005635856.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product PERQ amino acid-rich with GYF domain-containing protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635856.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGACTC TGTGTGGGGA ATGAACCACA GTGCACTCCA TTCAGTATTT
    CAGACCAATC AAAGCAACAA CCAACAATCC AATTTTGAGG CCGTACAAAG TGGCAAGAAG
    AAGAAAAAGC AGAAGATGGT CCGAGCAGAT CCCAGTTTGT TAGGATTTTC AGTCAATGCT
    TCATCAGAGC GACTCAATAT GGGTGAAATC GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635913.1
    CDS197..4087
    Translation

    Target ORF information:

    RefSeq Version XM_005635856.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635856.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGACTC TGTGTGGGGA ATGAACCACA GTGCACTCCA TTCAGTATTT
    CAGACCAATC AAAGCAACAA CCAACAATCC AATTTTGAGG CCGTACAAAG TGGCAAGAAG
    AAGAAAAAGC AGAAGATGGT CCGAGCAGAT CCCAGTTTGT TAGGATTTTC AGTCAATGCT
    TCATCAGAGC GACTCAATAT GGGTGAAATC GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25827
    Clone ID Related Accession (Same CDS sequence) XM_005635856.2 , XM_005635856.3
    Accession Version XM_005635856.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_005635856.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGACTC TGTGTGGGGA ATGAACCACA GTGCACTCCA TTCAGTATTT
    CAGACCAATC AAAGCAACAA CCAACAATCC AATTTTGAGG CCGTACAAAG TGGCAAGAAG
    AAGAAAAAGC AGAAGATGGT CCGAGCAGAT CCCAGTTTGT TAGGATTTTC AGTCAATGCT
    TCATCAGAGC GACTCAATAT GGGTGAAATC GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635913.1
    CDS215..4105
    Translation

    Target ORF information:

    RefSeq Version XM_005635856.3
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635856.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGACTC TGTGTGGGGA ATGAACCACA GTGCACTCCA TTCAGTATTT
    CAGACCAATC AAAGCAACAA CCAACAATCC AATTTTGAGG CCGTACAAAG TGGCAAGAAG
    AAGAAAAAGC AGAAGATGGT CCGAGCAGAT CCCAGTTTGT TAGGATTTTC AGTCAATGCT
    TCATCAGAGC GACTCAATAT GGGTGAAATC GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25828
    Clone ID Related Accession (Same CDS sequence) XM_856835.5 , XM_856835.4
    Accession Version XM_856835.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 5, 2017 this sequence version replaced XM_856835.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGGCTGGCGA
    GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG
    GGTTATCGAA GGGTGCGCTC TGGCAGTGGA AGCATAGACG ATGACCGGGA TAGTTTGCCC
    GAGTGGTGCT TAGAGGACGC TGAAGAAGAG ATGGGCACAT TTGACTCCTC TGGAGCATTC
    CTCTTTCTAA AGAAAGTACA GAAAGAGCCA ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGAGG AAGGTGGGGA GTGCTCTGAC TCTGAGAGCA GCCATAATGA AGAAGCCAAA
    GAACCTGATA AGACAAATAG GAAAGAGGGA GAGAAGACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CATCCCAGAC CTCATCATCA TCTGCAAGAC CAGGTACTCC TTCAGACCAT
    CAGCCTCAGG AAGCACCGCA ATTTGAGAGG AAGGAGGAAT CCCAAACTGA GCAAACGGAA
    AAAGCTGAGG AAGAGGGTCA GACGGAAAAT AGCCTGCCAA CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTTCTGATGT CCAGCAGCCC CTGTCACAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC GCCTGTTCCC AATCCTAGTC CTGCCCTCCG GCCAGTTGAA
    ACGCCGGTCG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAGCCAGA TGATGAAGAG
    GGTCTCAAGC ATTTGGAGCA GCAAGCTGAG AAGATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AGAGACTGGC ATCAAAACTG CAAGAGCACA GAGCTAAGGG AGTCTCAATT
    CCATTGATGC ACGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCGGAA TGGTTTCAGG CGGGTTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCATG TGATGAAAGC TTCCAACCTC TTGGTGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTCTC TCCCGGCCCA GCTCCCCCTC CTCATATGGG AGAGTTGGAC
    CAGGAACGAC TGACCAGGCA GCAGGAGCTC ACTGCCTTGT ACCAAATGCA GCACCTTCAG
    TACCAGCAGT TCTTAATACA ACAGCAGTAT GCACAGGTTT TGGCTCAACA ACAGAAAGCA
    GCTTTGTCTT CACAGCAGCA GCAGCAGTTG GCACTTCTTC TCCAACAGTT CCAGGCCCTG
    AAAATGAGAA TATCTGATCA GAACATCATT CCTTCAGTAA CTAGGTCTGT GTCGGTGCCA
    GATACTGGTT CTATCTGGGA GCTTCAACCA ACAGCCTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC CCTTGACACC ACAACTCCAG GACCAGCCCT TGAACAGCTT
    CAGCAGATGG AGAAGGCCAA AGCTGCAAAG CTAGAACAGG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA GGAACGAAAG AGGCAAGAAG AACTCCGGAG ACAACAGGAG
    GAAATTCTTC GGAGACAGCA GGAGGAGGAA AGGAAGAGGC GGGAGGAAGA AGAGCTCGCC
    CGAAGGAAAC AGGAAGAAGC TCTGCGACGC CAAAGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAACAGCAA GAAGAAGCTC TTAGGAGACT GGAGGAGAGG
    AGGAGAGAAG AGGAAGAAAG GCGGAAGCAG GAGGAGTTGT TACGCAAGCA GGAAGAGGAA
    GCTGCAAAAT GGGCCAGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGAATGGAAG AGGAGGCAGC CCGACTCCGG CATGAAGAAG AGGAGCGGAA GAGGAAAGAG
    TTAGAGCTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAACA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAACA GCAGCAGCAG CAGCTGGCTC AGATGAAGCT TCCCTCTTCT
    TCCACATGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCAAC GCTGTCATTG
    GCTGAAATCC AAAAACTAGA AGAGGAACGA GAACGGCAGC TTCGAGAGGA GCAAAGACGC
    CAGCAGAGGG AACTGATGAA AGCTCTGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAACTC
    TCTGGATGGG GGAATGTCAG TAAACCTGCA GGCACTGCGA AGTCTCTCTT GGAGATACAG
    CAGGAAGAAG CCAGGCAGAT GCAGAAGCAG CAACAGCAGC AGCAGCAGCA CCAGCAACCA
    AACAGAGCCC GGAATAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC CCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATCTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCTACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACC CAGTGGTGTG AGCAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACT TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATTAGGGCC TATTTAGGAG ATACCTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTCGA GCGCCGTGCC AAACAGAAAG CCAGCCAGCG GCAGCAGCAG
    CAGCAGCAAC AACAGCAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC
    TCTGTGTGGG GAATGAACCA CAGTGCACTC CATTCAGTAT TTCAGACCAA TCAAAGCAAC
    AACCAACAAT CCAATTTTGA GGCCGTACAA AGTGGCAAGA AGAAGAAAAA GCAGAAGATG
    GTCCGAGCAG ATCCCAGTTT GTTAGGATTT TCAGTCAATG CTTCATCAGA GCGACTCAAT
    ATGGGTGAAA TCGAGACTTT GGATGACTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_861928.1
    CDS215..4087
    Translation

    Target ORF information:

    RefSeq Version XM_856835.5
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_856835.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGGCTGGCGA
    GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG
    GGTTATCGAA GGGTGCGCTC TGGCAGTGGA AGCATAGACG ATGACCGGGA TAGTTTGCCC
    GAGTGGTGCT TAGAGGACGC TGAAGAAGAG ATGGGCACAT TTGACTCCTC TGGAGCATTC
    CTCTTTCTAA AGAAAGTACA GAAAGAGCCA ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGAGG AAGGTGGGGA GTGCTCTGAC TCTGAGAGCA GCCATAATGA AGAAGCCAAA
    GAACCTGATA AGACAAATAG GAAAGAGGGA GAGAAGACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CATCCCAGAC CTCATCATCA TCTGCAAGAC CAGGTACTCC TTCAGACCAT
    CAGCCTCAGG AAGCACCGCA ATTTGAGAGG AAGGAGGAAT CCCAAACTGA GCAAACGGAA
    AAAGCTGAGG AAGAGGGTCA GACGGAAAAT AGCCTGCCAA CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTTCTGATGT CCAGCAGCCC CTGTCACAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC GCCTGTTCCC AATCCTAGTC CTGCCCTCCG GCCAGTTGAA
    ACGCCGGTCG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAGCCAGA TGATGAAGAG
    GGTCTCAAGC ATTTGGAGCA GCAAGCTGAG AAGATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AGAGACTGGC ATCAAAACTG CAAGAGCACA GAGCTAAGGG AGTCTCAATT
    CCATTGATGC ACGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCGGAA TGGTTTCAGG CGGGTTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCATG TGATGAAAGC TTCCAACCTC TTGGTGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTCTC TCCCGGCCCA GCTCCCCCTC CTCATATGGG AGAGTTGGAC
    CAGGAACGAC TGACCAGGCA GCAGGAGCTC ACTGCCTTGT ACCAAATGCA GCACCTTCAG
    TACCAGCAGT TCTTAATACA ACAGCAGTAT GCACAGGTTT TGGCTCAACA ACAGAAAGCA
    GCTTTGTCTT CACAGCAGCA GCAGCAGTTG GCACTTCTTC TCCAACAGTT CCAGGCCCTG
    AAAATGAGAA TATCTGATCA GAACATCATT CCTTCAGTAA CTAGGTCTGT GTCGGTGCCA
    GATACTGGTT CTATCTGGGA GCTTCAACCA ACAGCCTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC CCTTGACACC ACAACTCCAG GACCAGCCCT TGAACAGCTT
    CAGCAGATGG AGAAGGCCAA AGCTGCAAAG CTAGAACAGG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA GGAACGAAAG AGGCAAGAAG AACTCCGGAG ACAACAGGAG
    GAAATTCTTC GGAGACAGCA GGAGGAGGAA AGGAAGAGGC GGGAGGAAGA AGAGCTCGCC
    CGAAGGAAAC AGGAAGAAGC TCTGCGACGC CAAAGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAACAGCAA GAAGAAGCTC TTAGGAGACT GGAGGAGAGG
    AGGAGAGAAG AGGAAGAAAG GCGGAAGCAG GAGGAGTTGT TACGCAAGCA GGAAGAGGAA
    GCTGCAAAAT GGGCCAGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGAATGGAAG AGGAGGCAGC CCGACTCCGG CATGAAGAAG AGGAGCGGAA GAGGAAAGAG
    TTAGAGCTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAACA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAACA GCAGCAGCAG CAGCTGGCTC AGATGAAGCT TCCCTCTTCT
    TCCACATGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCAAC GCTGTCATTG
    GCTGAAATCC AAAAACTAGA AGAGGAACGA GAACGGCAGC TTCGAGAGGA GCAAAGACGC
    CAGCAGAGGG AACTGATGAA AGCTCTGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAACTC
    TCTGGATGGG GGAATGTCAG TAAACCTGCA GGCACTGCGA AGTCTCTCTT GGAGATACAG
    CAGGAAGAAG CCAGGCAGAT GCAGAAGCAG CAACAGCAGC AGCAGCAGCA CCAGCAACCA
    AACAGAGCCC GGAATAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC CCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATCTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCTACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACC CAGTGGTGTG AGCAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACT TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATTAGGGCC TATTTAGGAG ATACCTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTCGA GCGCCGTGCC AAACAGAAAG CCAGCCAGCG GCAGCAGCAG
    CAGCAGCAAC AACAGCAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC
    TCTGTGTGGG GAATGAACCA CAGTGCACTC CATTCAGTAT TTCAGACCAA TCAAAGCAAC
    AACCAACAAT CCAATTTTGA GGCCGTACAA AGTGGCAAGA AGAAGAAAAA GCAGAAGATG
    GTCCGAGCAG ATCCCAGTTT GTTAGGATTT TCAGTCAATG CTTCATCAGA GCGACTCAAT
    ATGGGTGAAA TCGAGACTTT GGATGACTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25828
    Clone ID Related Accession (Same CDS sequence) XM_856835.5 , XM_856835.4
    Accession Version XM_856835.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3873bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product PERQ amino acid-rich with GYF domain-containing protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_856835.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGGCTGGCGA
    GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG
    GGTTATCGAA GGGTGCGCTC TGGCAGTGGA AGCATAGACG ATGACCGGGA TAGTTTGCCC
    GAGTGGTGCT TAGAGGACGC TGAAGAAGAG ATGGGCACAT TTGACTCCTC TGGAGCATTC
    CTCTTTCTAA AGAAAGTACA GAAAGAGCCA ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGAGG AAGGTGGGGA GTGCTCTGAC TCTGAGAGCA GCCATAATGA AGAAGCCAAA
    GAACCTGATA AGACAAATAG GAAAGAGGGA GAGAAGACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CATCCCAGAC CTCATCATCA TCTGCAAGAC CAGGTACTCC TTCAGACCAT
    CAGCCTCAGG AAGCACCGCA ATTTGAGAGG AAGGAGGAAT CCCAAACTGA GCAAACGGAA
    AAAGCTGAGG AAGAGGGTCA GACGGAAAAT AGCCTGCCAA CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTTCTGATGT CCAGCAGCCC CTGTCACAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC GCCTGTTCCC AATCCTAGTC CTGCCCTCCG GCCAGTTGAA
    ACGCCGGTCG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAGCCAGA TGATGAAGAG
    GGTCTCAAGC ATTTGGAGCA GCAAGCTGAG AAGATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AGAGACTGGC ATCAAAACTG CAAGAGCACA GAGCTAAGGG AGTCTCAATT
    CCATTGATGC ACGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCGGAA TGGTTTCAGG CGGGTTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCATG TGATGAAAGC TTCCAACCTC TTGGTGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTCTC TCCCGGCCCA GCTCCCCCTC CTCATATGGG AGAGTTGGAC
    CAGGAACGAC TGACCAGGCA GCAGGAGCTC ACTGCCTTGT ACCAAATGCA GCACCTTCAG
    TACCAGCAGT TCTTAATACA ACAGCAGTAT GCACAGGTTT TGGCTCAACA ACAGAAAGCA
    GCTTTGTCTT CACAGCAGCA GCAGCAGTTG GCACTTCTTC TCCAACAGTT CCAGGCCCTG
    AAAATGAGAA TATCTGATCA GAACATCATT CCTTCAGTAA CTAGGTCTGT GTCGGTGCCA
    GATACTGGTT CTATCTGGGA GCTTCAACCA ACAGCCTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC CCTTGACACC ACAACTCCAG GACCAGCCCT TGAACAGCTT
    CAGCAGATGG AGAAGGCCAA AGCTGCAAAG CTAGAACAGG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA GGAACGAAAG AGGCAAGAAG AACTCCGGAG ACAACAGGAG
    GAAATTCTTC GGAGACAGCA GGAGGAGGAA AGGAAGAGGC GGGAGGAAGA AGAGCTCGCC
    CGAAGGAAAC AGGAAGAAGC TCTGCGACGC CAAAGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAACAGCAA GAAGAAGCTC TTAGGAGACT GGAGGAGAGG
    AGGAGAGAAG AGGAAGAAAG GCGGAAGCAG GAGGAGTTGT TACGCAAGCA GGAAGAGGAA
    GCTGCAAAAT GGGCCAGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGAATGGAAG AGGAGGCAGC CCGACTCCGG CATGAAGAAG AGGAGCGGAA GAGGAAAGAG
    TTAGAGCTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAACA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAACA GCAGCAGCAG CAGCTGGCTC AGATGAAGCT TCCCTCTTCT
    TCCACATGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCAAC GCTGTCATTG
    GCTGAAATCC AAAAACTAGA AGAGGAACGA GAACGGCAGC TTCGAGAGGA GCAAAGACGC
    CAGCAGAGGG AACTGATGAA AGCTCTGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAACTC
    TCTGGATGGG GGAATGTCAG TAAACCTGCA GGCACTGCGA AGTCTCTCTT GGAGATACAG
    CAGGAAGAAG CCAGGCAGAT GCAGAAGCAG CAACAGCAGC AGCAGCAGCA CCAGCAACCA
    AACAGAGCCC GGAATAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC CCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATCTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCTACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACC CAGTGGTGTG AGCAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACT TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATTAGGGCC TATTTAGGAG ATACCTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTCGA GCGCCGTGCC AAACAGAAAG CCAGCCAGCG GCAGCAGCAG
    CAGCAGCAAC AACAGCAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC
    TCTGTGTGGG GAATGAACCA CAGTGCACTC CATTCAGTAT TTCAGACCAA TCAAAGCAAC
    AACCAACAAT CCAATTTTGA GGCCGTACAA AGTGGCAAGA AGAAGAAAAA GCAGAAGATG
    GTCCGAGCAG ATCCCAGTTT GTTAGGATTT TCAGTCAATG CTTCATCAGA GCGACTCAAT
    ATGGGTGAAA TCGAGACTTT GGATGACTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_861928.1
    CDS197..4069
    Translation

    Target ORF information:

    RefSeq Version XM_856835.4
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_856835.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGGCTGGCGA
    GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG
    GGTTATCGAA GGGTGCGCTC TGGCAGTGGA AGCATAGACG ATGACCGGGA TAGTTTGCCC
    GAGTGGTGCT TAGAGGACGC TGAAGAAGAG ATGGGCACAT TTGACTCCTC TGGAGCATTC
    CTCTTTCTAA AGAAAGTACA GAAAGAGCCA ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGAGG AAGGTGGGGA GTGCTCTGAC TCTGAGAGCA GCCATAATGA AGAAGCCAAA
    GAACCTGATA AGACAAATAG GAAAGAGGGA GAGAAGACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CATCCCAGAC CTCATCATCA TCTGCAAGAC CAGGTACTCC TTCAGACCAT
    CAGCCTCAGG AAGCACCGCA ATTTGAGAGG AAGGAGGAAT CCCAAACTGA GCAAACGGAA
    AAAGCTGAGG AAGAGGGTCA GACGGAAAAT AGCCTGCCAA CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTTCTGATGT CCAGCAGCCC CTGTCACAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC GCCTGTTCCC AATCCTAGTC CTGCCCTCCG GCCAGTTGAA
    ACGCCGGTCG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAGCCAGA TGATGAAGAG
    GGTCTCAAGC ATTTGGAGCA GCAAGCTGAG AAGATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AGAGACTGGC ATCAAAACTG CAAGAGCACA GAGCTAAGGG AGTCTCAATT
    CCATTGATGC ACGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCGGAA TGGTTTCAGG CGGGTTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCATG TGATGAAAGC TTCCAACCTC TTGGTGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTCTC TCCCGGCCCA GCTCCCCCTC CTCATATGGG AGAGTTGGAC
    CAGGAACGAC TGACCAGGCA GCAGGAGCTC ACTGCCTTGT ACCAAATGCA GCACCTTCAG
    TACCAGCAGT TCTTAATACA ACAGCAGTAT GCACAGGTTT TGGCTCAACA ACAGAAAGCA
    GCTTTGTCTT CACAGCAGCA GCAGCAGTTG GCACTTCTTC TCCAACAGTT CCAGGCCCTG
    AAAATGAGAA TATCTGATCA GAACATCATT CCTTCAGTAA CTAGGTCTGT GTCGGTGCCA
    GATACTGGTT CTATCTGGGA GCTTCAACCA ACAGCCTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC CCTTGACACC ACAACTCCAG GACCAGCCCT TGAACAGCTT
    CAGCAGATGG AGAAGGCCAA AGCTGCAAAG CTAGAACAGG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA GGAACGAAAG AGGCAAGAAG AACTCCGGAG ACAACAGGAG
    GAAATTCTTC GGAGACAGCA GGAGGAGGAA AGGAAGAGGC GGGAGGAAGA AGAGCTCGCC
    CGAAGGAAAC AGGAAGAAGC TCTGCGACGC CAAAGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAACAGCAA GAAGAAGCTC TTAGGAGACT GGAGGAGAGG
    AGGAGAGAAG AGGAAGAAAG GCGGAAGCAG GAGGAGTTGT TACGCAAGCA GGAAGAGGAA
    GCTGCAAAAT GGGCCAGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGAATGGAAG AGGAGGCAGC CCGACTCCGG CATGAAGAAG AGGAGCGGAA GAGGAAAGAG
    TTAGAGCTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAACA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAACA GCAGCAGCAG CAGCTGGCTC AGATGAAGCT TCCCTCTTCT
    TCCACATGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCAAC GCTGTCATTG
    GCTGAAATCC AAAAACTAGA AGAGGAACGA GAACGGCAGC TTCGAGAGGA GCAAAGACGC
    CAGCAGAGGG AACTGATGAA AGCTCTGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAACTC
    TCTGGATGGG GGAATGTCAG TAAACCTGCA GGCACTGCGA AGTCTCTCTT GGAGATACAG
    CAGGAAGAAG CCAGGCAGAT GCAGAAGCAG CAACAGCAGC AGCAGCAGCA CCAGCAACCA
    AACAGAGCCC GGAATAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC CCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATCTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCTACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACC CAGTGGTGTG AGCAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACT TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATTAGGGCC TATTTAGGAG ATACCTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTCGA GCGCCGTGCC AAACAGAAAG CCAGCCAGCG GCAGCAGCAG
    CAGCAGCAAC AACAGCAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC
    TCTGTGTGGG GAATGAACCA CAGTGCACTC CATTCAGTAT TTCAGACCAA TCAAAGCAAC
    AACCAACAAT CCAATTTTGA GGCCGTACAA AGTGGCAAGA AGAAGAAAAA GCAGAAGATG
    GTCCGAGCAG ATCCCAGTTT GTTAGGATTT TCAGTCAATG CTTCATCAGA GCGACTCAAT
    ATGGGTGAAA TCGAGACTTT GGATGACTAC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25829
    Clone ID Related Accession (Same CDS sequence) XM_005635857.2
    Accession Version XM_005635857.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3738bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product PERQ amino acid-rich with GYF domain-containing protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635857.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG GAGACCAAAT TTTGAGGAAG GTGGACCAAC ATCGGTGGGA
    AGAAAGCATG AGTTTATACG ATCAGAAAGT GAAAACTGGC GTATTTTTAG AGAAGAACAA
    AACGGAGAAG ATGAAGATGG AGGTTGGAGA CTAGCTGGAC CGAGACGGGA TGGAGAGAGG
    TGGCGTCCCC ATAGCCCTGA TGGCCCTCGT TCTGCAGGCT GGCGAGAACA CATGGAACGA
    CGGCGAAGGT TTGAGTTTGA TTTTCGAGAT CGGGATGATG AACGGGGTTA TCGAAGGGTG
    CGCTCTGGCA GTGGAAGCAT AGACGATGAC CGGGATAGTT TGCCCGAGTG GTGCTTAGAG
    GACGCTGAAG AAGAGATGGG CACATTTGAC TCCTCTGGAG CATTCCTCTT TCTAAAGAAA
    GTACAGAAAG AGCCAATTCC AGAAGAGCAG GAGATGGACT TCCGGCCTGT GGAGGAAGGT
    GGGGAGTGCT CTGACTCTGA GAGCAGCCAT AATGAAGAAG CCAAAGAACC TGATAAGACA
    AATAGGAAAG AGGGAGAGAA GACAGATAGA GTAGGAGTTG AAGCTAGTGA GGAAACATCC
    CAGACCTCAT CATCATCTGC AAGACCAGGT ACTCCTTCAG ACCATCAGCC TCAGGAAGCA
    CCGCAATTTG AGAGGAAGGA GGAATCCCAA ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG
    GGTCAGACGG AAAATAGCCT GCCAACCAAA GTGCCCAGCA GAGGGGATGA AATGGTTTCT
    GATGTCCAGC AGCCCCTGTC ACAGATTCCT TCAGATACAG CCTCTCCTCT TCTCATACTT
    CCACCGCCTG TTCCCAATCC TAGTCCTGCC CTCCGGCCAG TTGAAACGCC GGTCGTAGGT
    GCTCCTGGTA TGGGCAGTGT TTCCACAGAG CCAGATGATG AAGAGGGTCT CAAGCATTTG
    GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT CTCCAAGACA GTGCACTAGA TGATGAGAGA
    CTGGCATCAA AACTGCAAGA GCACAGAGCT AAGGGAGTCT CAATTCCATT GATGCACGAA
    GCAATGCAGA AGTGGTATTA CAAAGATCCT CAGGGAGAAA TTCAAGGTCC CTTCAATAAT
    CAGGAGATGG CGGAATGGTT TCAGGCGGGT TATTTTACTA TGTCTTTATT GGTGAAGAGA
    GCATGTGATG AAAGCTTCCA ACCTCTTGGT GATATCATGA AAATGTGGGG AAGGGTTCCC
    TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT ATGGGAGAGT TGGACCAGGA ACGACTGACC
    AGGCAGCAGG AGCTCACTGC CTTGTACCAA ATGCAGCACC TTCAGTACCA GCAGTTCTTA
    ATACAACAGC AGTATGCACA GGTTTTGGCT CAACAACAGA AAGCAGCTTT GTCTTCACAG
    CAGCAGCAGC AGTTGGCACT TCTTCTCCAA CAGTTCCAGG CCCTGAAAAT GAGAATATCT
    GATCAGAACA TCATTCCTTC AGTAACTAGG TCTGTGTCGG TGCCAGATAC TGGTTCTATC
    TGGGAGCTTC AACCAACAGC CTCACAGCCT ACAGTTTGGG AAGGTGGTAG TGTATGGGAT
    CTTCCCCTTG ACACCACAAC TCCAGGACCA GCCCTTGAAC AGCTTCAGCA GATGGAGAAG
    GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA AGAGAGGCAG AAATGAGGGC AAAACGGGAA
    GAGGAGGAAC GAAAGAGGCA AGAAGAACTC CGGAGACAAC AGGAGGAAAT TCTTCGGAGA
    CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG GAAGAAGAGC TCGCCCGAAG GAAACAGGAA
    GAAGCTCTGC GACGCCAAAG GGAGCAAGAA ATTGCATTAA GGCGACAGCG AGAAGAGGAA
    GAAAGACAAC AGCAAGAAGA AGCTCTTAGG AGACTGGAGG AGAGGAGGAG AGAAGAGGAA
    GAAAGGCGGA AGCAGGAGGA GTTGTTACGC AAGCAGGAAG AGGAAGCTGC AAAATGGGCC
    AGGGAAGAAG AAGAAGCCCA GCGTCGATTA GAGGAGAACC GGCTGCGAAT GGAAGAGGAG
    GCAGCCCGAC TCCGGCATGA AGAAGAGGAG CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG
    CAGAAGGAGT TAATGCGCCA GAGGCAGCAG CAACAAGAGG CTCTCCGGAG GTTGCAGCAG
    CAACAGCAGC AGCAGCAGCT GGCTCAGATG AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG
    CAGTCCAATA CAACAGCATG TCAGTCCCAG GCAACGCTGT CATTGGCTGA AATCCAAAAA
    CTAGAAGAGG AACGAGAACG GCAGCTTCGA GAGGAGCAAA GACGCCAGCA GAGGGAACTG
    ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG CAGCAGCAGA AACTCTCTGG ATGGGGGAAT
    GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT CTCTTGGAGA TACAGCAGGA AGAAGCCAGG
    CAGATGCAGA AGCAGCAACA GCAGCAGCAG CAGCACCAGC AACCAAACAG AGCCCGGAAT
    AATACGCATT CCAACCTGCA CACCAGCATT GGGAATTCTG TTTGGGGCTC TATAAATACT
    GGTCCCCCTA ACCAGTGGGC ATCTGACCTA GTCAGTAGTA TCTGGAGTAA TGCTGACACT
    AAAAACTCCA ACATGGGATT CTGGGATGAT GCAGTGAAAG AGGTGGGACC TAGGAATTCT
    ACAAATAAAA ATAAAAACAA CGCCAGTCTC AGTAAATCTG TAGGTGTGTC TAACCGGCAG
    AATAAGAAAG TAGAAGAAGA AGAAAAGTTG CTGAAGCTCT TTCAGGGAGT AAATAAAGCC
    CAAGATGGAT TTACCCAGTG GTGTGAGCAG ATGCTTCATG CCCTTAATAC GGCAAATAAC
    TTGGATGTTC CCACTTTTGT TTCTTTCCTG AAAGAAGTAG AATCTCCTTA TGAGGTCCAT
    GATTATATTA GGGCCTATTT AGGAGATACC TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC
    CTCGAGCGCC GTGCCAAACA GAAAGCCAGC CAGCGGCAGC AGCAGCAGCA GCAACAACAG
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGGACTCTGT GTGGGGAATG
    AACCACAGTG CACTCCATTC AGTATTTCAG ACCAATCAAA GCAACAACCA ACAATCCAAT
    TTTGAGGCCG TACAAAGTGG CAAGAAGAAG AAAAAGCAGA AGATGGTCCG AGCAGATCCC
    AGTTTGTTAG GATTTTCAGT CAATGCTTCA TCAGAGCGAC TCAATATGGG TGAAATCGAG
    ACTTTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635914.1
    CDS197..3934
    Translation

    Target ORF information:

    RefSeq Version XM_005635857.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635857.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG GAGACCAAAT TTTGAGGAAG GTGGACCAAC ATCGGTGGGA
    AGAAAGCATG AGTTTATACG ATCAGAAAGT GAAAACTGGC GTATTTTTAG AGAAGAACAA
    AACGGAGAAG ATGAAGATGG AGGTTGGAGA CTAGCTGGAC CGAGACGGGA TGGAGAGAGG
    TGGCGTCCCC ATAGCCCTGA TGGCCCTCGT TCTGCAGGCT GGCGAGAACA CATGGAACGA
    CGGCGAAGGT TTGAGTTTGA TTTTCGAGAT CGGGATGATG AACGGGGTTA TCGAAGGGTG
    CGCTCTGGCA GTGGAAGCAT AGACGATGAC CGGGATAGTT TGCCCGAGTG GTGCTTAGAG
    GACGCTGAAG AAGAGATGGG CACATTTGAC TCCTCTGGAG CATTCCTCTT TCTAAAGAAA
    GTACAGAAAG AGCCAATTCC AGAAGAGCAG GAGATGGACT TCCGGCCTGT GGAGGAAGGT
    GGGGAGTGCT CTGACTCTGA GAGCAGCCAT AATGAAGAAG CCAAAGAACC TGATAAGACA
    AATAGGAAAG AGGGAGAGAA GACAGATAGA GTAGGAGTTG AAGCTAGTGA GGAAACATCC
    CAGACCTCAT CATCATCTGC AAGACCAGGT ACTCCTTCAG ACCATCAGCC TCAGGAAGCA
    CCGCAATTTG AGAGGAAGGA GGAATCCCAA ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG
    GGTCAGACGG AAAATAGCCT GCCAACCAAA GTGCCCAGCA GAGGGGATGA AATGGTTTCT
    GATGTCCAGC AGCCCCTGTC ACAGATTCCT TCAGATACAG CCTCTCCTCT TCTCATACTT
    CCACCGCCTG TTCCCAATCC TAGTCCTGCC CTCCGGCCAG TTGAAACGCC GGTCGTAGGT
    GCTCCTGGTA TGGGCAGTGT TTCCACAGAG CCAGATGATG AAGAGGGTCT CAAGCATTTG
    GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT CTCCAAGACA GTGCACTAGA TGATGAGAGA
    CTGGCATCAA AACTGCAAGA GCACAGAGCT AAGGGAGTCT CAATTCCATT GATGCACGAA
    GCAATGCAGA AGTGGTATTA CAAAGATCCT CAGGGAGAAA TTCAAGGTCC CTTCAATAAT
    CAGGAGATGG CGGAATGGTT TCAGGCGGGT TATTTTACTA TGTCTTTATT GGTGAAGAGA
    GCATGTGATG AAAGCTTCCA ACCTCTTGGT GATATCATGA AAATGTGGGG AAGGGTTCCC
    TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT ATGGGAGAGT TGGACCAGGA ACGACTGACC
    AGGCAGCAGG AGCTCACTGC CTTGTACCAA ATGCAGCACC TTCAGTACCA GCAGTTCTTA
    ATACAACAGC AGTATGCACA GGTTTTGGCT CAACAACAGA AAGCAGCTTT GTCTTCACAG
    CAGCAGCAGC AGTTGGCACT TCTTCTCCAA CAGTTCCAGG CCCTGAAAAT GAGAATATCT
    GATCAGAACA TCATTCCTTC AGTAACTAGG TCTGTGTCGG TGCCAGATAC TGGTTCTATC
    TGGGAGCTTC AACCAACAGC CTCACAGCCT ACAGTTTGGG AAGGTGGTAG TGTATGGGAT
    CTTCCCCTTG ACACCACAAC TCCAGGACCA GCCCTTGAAC AGCTTCAGCA GATGGAGAAG
    GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA AGAGAGGCAG AAATGAGGGC AAAACGGGAA
    GAGGAGGAAC GAAAGAGGCA AGAAGAACTC CGGAGACAAC AGGAGGAAAT TCTTCGGAGA
    CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG GAAGAAGAGC TCGCCCGAAG GAAACAGGAA
    GAAGCTCTGC GACGCCAAAG GGAGCAAGAA ATTGCATTAA GGCGACAGCG AGAAGAGGAA
    GAAAGACAAC AGCAAGAAGA AGCTCTTAGG AGACTGGAGG AGAGGAGGAG AGAAGAGGAA
    GAAAGGCGGA AGCAGGAGGA GTTGTTACGC AAGCAGGAAG AGGAAGCTGC AAAATGGGCC
    AGGGAAGAAG AAGAAGCCCA GCGTCGATTA GAGGAGAACC GGCTGCGAAT GGAAGAGGAG
    GCAGCCCGAC TCCGGCATGA AGAAGAGGAG CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG
    CAGAAGGAGT TAATGCGCCA GAGGCAGCAG CAACAAGAGG CTCTCCGGAG GTTGCAGCAG
    CAACAGCAGC AGCAGCAGCT GGCTCAGATG AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG
    CAGTCCAATA CAACAGCATG TCAGTCCCAG GCAACGCTGT CATTGGCTGA AATCCAAAAA
    CTAGAAGAGG AACGAGAACG GCAGCTTCGA GAGGAGCAAA GACGCCAGCA GAGGGAACTG
    ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG CAGCAGCAGA AACTCTCTGG ATGGGGGAAT
    GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT CTCTTGGAGA TACAGCAGGA AGAAGCCAGG
    CAGATGCAGA AGCAGCAACA GCAGCAGCAG CAGCACCAGC AACCAAACAG AGCCCGGAAT
    AATACGCATT CCAACCTGCA CACCAGCATT GGGAATTCTG TTTGGGGCTC TATAAATACT
    GGTCCCCCTA ACCAGTGGGC ATCTGACCTA GTCAGTAGTA TCTGGAGTAA TGCTGACACT
    AAAAACTCCA ACATGGGATT CTGGGATGAT GCAGTGAAAG AGGTGGGACC TAGGAATTCT
    ACAAATAAAA ATAAAAACAA CGCCAGTCTC AGTAAATCTG TAGGTGTGTC TAACCGGCAG
    AATAAGAAAG TAGAAGAAGA AGAAAAGTTG CTGAAGCTCT TTCAGGGAGT AAATAAAGCC
    CAAGATGGAT TTACCCAGTG GTGTGAGCAG ATGCTTCATG CCCTTAATAC GGCAAATAAC
    TTGGATGTTC CCACTTTTGT TTCTTTCCTG AAAGAAGTAG AATCTCCTTA TGAGGTCCAT
    GATTATATTA GGGCCTATTT AGGAGATACC TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC
    CTCGAGCGCC GTGCCAAACA GAAAGCCAGC CAGCGGCAGC AGCAGCAGCA GCAACAACAG
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGGACTCTGT GTGGGGAATG
    AACCACAGTG CACTCCATTC AGTATTTCAG ACCAATCAAA GCAACAACCA ACAATCCAAT
    TTTGAGGCCG TACAAAGTGG CAAGAAGAAG AAAAAGCAGA AGATGGTCCG AGCAGATCCC
    AGTTTGTTAG GATTTTCAGT CAATGCTTCA TCAGAGCGAC TCAATATGGG TGAAATCGAG
    ACTTTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe25830
    Clone ID Related Accession (Same CDS sequence) XM_005635858.2
    Accession Version XM_005635858.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2015-09-16
    Organism Canis lupus familiaris(Dog)
    Product PERQ amino acid-rich with GYF domain-containing protein 2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003726103.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 17, 2015 this sequence version replaced XM_005635858.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACAGGG TCTGGACTGA GCGAGAAGAG AAATTCCAGC GGATCAAAAA CAAACAAGAT 
    GGCCCTCGTT CTGCAGGCTG GCGAGAACAC ATGGAACGAC GGCGAAGGTT TGAGTTTGAT
    TTTCGAGATC GGGATGATGA ACGGGGTTAT CGAAGGGTGC GCTCTGGCAG TGGAAGCATA
    GACGATGACC GGGATAGTTT GCCCGAGTGG TGCTTAGAGG ACGCTGAAGA AGAGATGGGC
    ACATTTGACT CCTCTGGAGC ATTCCTCTTT CTAAAGAAAG TACAGAAAGA GCCAATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GAGGAAGGTG GGGAGTGCTC TGACTCTGAG
    AGCAGCCATA ATGAAGAAGC CAAAGAACCT GATAAGACAA ATAGGAAAGA GGGAGAGAAG
    ACAGATAGAG TAGGAGTTGA AGCTAGTGAG GAAACATCCC AGACCTCATC ATCATCTGCA
    AGACCAGGTA CTCCTTCAGA CCATCAGCCT CAGGAAGCAC CGCAATTTGA GAGGAAGGAG
    GAATCCCAAA CTGAGCAAAC GGAAAAAGCT GAGGAAGAGG GTCAGACGGA AAATAGCCTG
    CCAACCAAAG TGCCCAGCAG AGGGGATGAA ATGGTTTCTG ATGTCCAGCA GCCCCTGTCA
    CAGATTCCTT CAGATACAGC CTCTCCTCTT CTCATACTTC CACCGCCTGT TCCCAATCCT
    AGTCCTGCCC TCCGGCCAGT TGAAACGCCG GTCGTAGGTG CTCCTGGTAT GGGCAGTGTT
    TCCACAGAGC CAGATGATGA AGAGGGTCTC AAGCATTTGG AGCAGCAAGC TGAGAAGATG
    GTGGCTTATC TCCAAGACAG TGCACTAGAT GATGAGAGAC TGGCATCAAA ACTGCAAGAG
    CACAGAGCTA AGGGAGTCTC AATTCCATTG ATGCACGAAG CAATGCAGAA GTGGTATTAC
    AAAGATCCTC AGGGAGAAAT TCAAGGTCCC TTCAATAATC AGGAGATGGC GGAATGGTTT
    CAGGCGGGTT ATTTTACTAT GTCTTTATTG GTGAAGAGAG CATGTGATGA AAGCTTCCAA
    CCTCTTGGTG ATATCATGAA AATGTGGGGA AGGGTTCCCT TCTCTCCCGG CCCAGCTCCC
    CCTCCTCATA TGGGAGAGTT GGACCAGGAA CGACTGACCA GGCAGCAGGA GCTCACTGCC
    TTGTACCAAA TGCAGCACCT TCAGTACCAG CAGTTCTTAA TACAACAGCA GTATGCACAG
    GTTTTGGCTC AACAACAGAA AGCAGCTTTG TCTTCACAGC AGCAGCAGCA GTTGGCACTT
    CTTCTCCAAC AGTTCCAGGC CCTGAAAATG AGAATATCTG ATCAGAACAT CATTCCTTCA
    GTAACTAGGT CTGTGTCGGT GCCAGATACT GGTTCTATCT GGGAGCTTCA ACCAACAGCC
    TCACAGCCTA CAGTTTGGGA AGGTGGTAGT GTATGGGATC TTCCCCTTGA CACCACAACT
    CCAGGACCAG CCCTTGAACA GCTTCAGCAG ATGGAGAAGG CCAAAGCTGC AAAGCTAGAA
    CAGGAGAGAA GAGAGGCAGA AATGAGGGCA AAACGGGAAG AGGAGGAACG AAAGAGGCAA
    GAAGAACTCC GGAGACAACA GGAGGAAATT CTTCGGAGAC AGCAGGAGGA GGAAAGGAAG
    AGGCGGGAGG AAGAAGAGCT CGCCCGAAGG AAACAGGAAG AAGCTCTGCG ACGCCAAAGG
    GAGCAAGAAA TTGCATTAAG GCGACAGCGA GAAGAGGAAG AAAGACAACA GCAAGAAGAA
    GCTCTTAGGA GACTGGAGGA GAGGAGGAGA GAAGAGGAAG AAAGGCGGAA GCAGGAGGAG
    TTGTTACGCA AGCAGGAAGA GGAAGCTGCA AAATGGGCCA GGGAAGAAGA AGAAGCCCAG
    CGTCGATTAG AGGAGAACCG GCTGCGAATG GAAGAGGAGG CAGCCCGACT CCGGCATGAA
    GAAGAGGAGC GGAAGAGGAA AGAGTTAGAG CTCCAGCGGC AGAAGGAGTT AATGCGCCAG
    AGGCAGCAGC AACAAGAGGC TCTCCGGAGG TTGCAGCAGC AACAGCAGCA GCAGCAGCTG
    GCTCAGATGA AGCTTCCCTC TTCTTCCACA TGGGGCCAGC AGTCCAATAC AACAGCATGT
    CAGTCCCAGG CAACGCTGTC ATTGGCTGAA ATCCAAAAAC TAGAAGAGGA ACGAGAACGG
    CAGCTTCGAG AGGAGCAAAG ACGCCAGCAG AGGGAACTGA TGAAAGCTCT GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGAA ACTCTCTGGA TGGGGGAATG TCAGTAAACC TGCAGGCACT
    GCGAAGTCTC TCTTGGAGAT ACAGCAGGAA GAAGCCAGGC AGATGCAGAA GCAGCAACAG
    CAGCAGCAGC AGCACCAGCA ACCAAACAGA GCCCGGAATA ATACGCATTC CAACCTGCAC
    ACCAGCATTG GGAATTCTGT TTGGGGCTCT ATAAATACTG GTCCCCCTAA CCAGTGGGCA
    TCTGACCTAG TCAGTAGTAT CTGGAGTAAT GCTGACACTA AAAACTCCAA CATGGGATTC
    TGGGATGATG CAGTGAAAGA GGTGGGACCT AGGAATTCTA CAAATAAAAA TAAAAACAAC
    GCCAGTCTCA GTAAATCTGT AGGTGTGTCT AACCGGCAGA ATAAGAAAGT AGAAGAAGAA
    GAAAAGTTGC TGAAGCTCTT TCAGGGAGTA AATAAAGCCC AAGATGGATT TACCCAGTGG
    TGTGAGCAGA TGCTTCATGC CCTTAATACG GCAAATAACT TGGATGTTCC CACTTTTGTT
    TCTTTCCTGA AAGAAGTAGA ATCTCCTTAT GAGGTCCATG ATTATATTAG GGCCTATTTA
    GGAGATACCT CTGAGGCCAA GGAGTTTGCC AAGCAGTTCC TCGAGCGCCG TGCCAAACAG
    AAAGCCAGCC AGCGGCAGCA GCAGCAGCAG CAACAACAGC AACAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GGACTCTGTG TGGGGAATGA ACCACAGTGC ACTCCATTCA
    GTATTTCAGA CCAATCAAAG CAACAACCAA CAATCCAATT TTGAGGCCGT ACAAAGTGGC
    AAGAAGAAGA AAAAGCAGAA GATGGTCCGA GCAGATCCCA GTTTGTTAGG ATTTTCAGTC
    AATGCTTCAT CAGAGCGACT CAATATGGGT GAAATCGAGA CTTTGGATGA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005635915.1
    CDS84..3320
    Translation

    Target ORF information:

    RefSeq Version XM_005635858.2
    Organism Canis lupus familiaris(Dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005635858.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACAGGG TCTGGACTGA GCGAGAAGAG AAATTCCAGC GGATCAAAAA CAAACAAGAT 
    GGCCCTCGTT CTGCAGGCTG GCGAGAACAC ATGGAACGAC GGCGAAGGTT TGAGTTTGAT
    TTTCGAGATC GGGATGATGA ACGGGGTTAT CGAAGGGTGC GCTCTGGCAG TGGAAGCATA
    GACGATGACC GGGATAGTTT GCCCGAGTGG TGCTTAGAGG ACGCTGAAGA AGAGATGGGC
    ACATTTGACT CCTCTGGAGC ATTCCTCTTT CTAAAGAAAG TACAGAAAGA GCCAATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GAGGAAGGTG GGGAGTGCTC TGACTCTGAG
    AGCAGCCATA ATGAAGAAGC CAAAGAACCT GATAAGACAA ATAGGAAAGA GGGAGAGAAG
    ACAGATAGAG TAGGAGTTGA AGCTAGTGAG GAAACATCCC AGACCTCATC ATCATCTGCA
    AGACCAGGTA CTCCTTCAGA CCATCAGCCT CAGGAAGCAC CGCAATTTGA GAGGAAGGAG
    GAATCCCAAA CTGAGCAAAC GGAAAAAGCT GAGGAAGAGG GTCAGACGGA AAATAGCCTG
    CCAACCAAAG TGCCCAGCAG AGGGGATGAA ATGGTTTCTG ATGTCCAGCA GCCCCTGTCA
    CAGATTCCTT CAGATACAGC CTCTCCTCTT CTCATACTTC CACCGCCTGT TCCCAATCCT
    AGTCCTGCCC TCCGGCCAGT TGAAACGCCG GTCGTAGGTG CTCCTGGTAT GGGCAGTGTT
    TCCACAGAGC CAGATGATGA AGAGGGTCTC AAGCATTTGG AGCAGCAAGC TGAGAAGATG
    GTGGCTTATC TCCAAGACAG TGCACTAGAT GATGAGAGAC TGGCATCAAA ACTGCAAGAG
    CACAGAGCTA AGGGAGTCTC AATTCCATTG ATGCACGAAG CAATGCAGAA GTGGTATTAC
    AAAGATCCTC AGGGAGAAAT TCAAGGTCCC TTCAATAATC AGGAGATGGC GGAATGGTTT
    CAGGCGGGTT ATTTTACTAT GTCTTTATTG GTGAAGAGAG CATGTGATGA AAGCTTCCAA
    CCTCTTGGTG ATATCATGAA AATGTGGGGA AGGGTTCCCT TCTCTCCCGG CCCAGCTCCC
    CCTCCTCATA TGGGAGAGTT GGACCAGGAA CGACTGACCA GGCAGCAGGA GCTCACTGCC
    TTGTACCAAA TGCAGCACCT TCAGTACCAG CAGTTCTTAA TACAACAGCA GTATGCACAG
    GTTTTGGCTC AACAACAGAA AGCAGCTTTG TCTTCACAGC AGCAGCAGCA GTTGGCACTT
    CTTCTCCAAC AGTTCCAGGC CCTGAAAATG AGAATATCTG ATCAGAACAT CATTCCTTCA
    GTAACTAGGT CTGTGTCGGT GCCAGATACT GGTTCTATCT GGGAGCTTCA ACCAACAGCC
    TCACAGCCTA CAGTTTGGGA AGGTGGTAGT GTATGGGATC TTCCCCTTGA CACCACAACT
    CCAGGACCAG CCCTTGAACA GCTTCAGCAG ATGGAGAAGG CCAAAGCTGC AAAGCTAGAA
    CAGGAGAGAA GAGAGGCAGA AATGAGGGCA AAACGGGAAG AGGAGGAACG AAAGAGGCAA
    GAAGAACTCC GGAGACAACA GGAGGAAATT CTTCGGAGAC AGCAGGAGGA GGAAAGGAAG
    AGGCGGGAGG AAGAAGAGCT CGCCCGAAGG AAACAGGAAG AAGCTCTGCG ACGCCAAAGG
    GAGCAAGAAA TTGCATTAAG GCGACAGCGA GAAGAGGAAG AAAGACAACA GCAAGAAGAA
    GCTCTTAGGA GACTGGAGGA GAGGAGGAGA GAAGAGGAAG AAAGGCGGAA GCAGGAGGAG
    TTGTTACGCA AGCAGGAAGA GGAAGCTGCA AAATGGGCCA GGGAAGAAGA AGAAGCCCAG
    CGTCGATTAG AGGAGAACCG GCTGCGAATG GAAGAGGAGG CAGCCCGACT CCGGCATGAA
    GAAGAGGAGC GGAAGAGGAA AGAGTTAGAG CTCCAGCGGC AGAAGGAGTT AATGCGCCAG
    AGGCAGCAGC AACAAGAGGC TCTCCGGAGG TTGCAGCAGC AACAGCAGCA GCAGCAGCTG
    GCTCAGATGA AGCTTCCCTC TTCTTCCACA TGGGGCCAGC AGTCCAATAC AACAGCATGT
    CAGTCCCAGG CAACGCTGTC ATTGGCTGAA ATCCAAAAAC TAGAAGAGGA ACGAGAACGG
    CAGCTTCGAG AGGAGCAAAG ACGCCAGCAG AGGGAACTGA TGAAAGCTCT GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGAA ACTCTCTGGA TGGGGGAATG TCAGTAAACC TGCAGGCACT
    GCGAAGTCTC TCTTGGAGAT ACAGCAGGAA GAAGCCAGGC AGATGCAGAA GCAGCAACAG
    CAGCAGCAGC AGCACCAGCA ACCAAACAGA GCCCGGAATA ATACGCATTC CAACCTGCAC
    ACCAGCATTG GGAATTCTGT TTGGGGCTCT ATAAATACTG GTCCCCCTAA CCAGTGGGCA
    TCTGACCTAG TCAGTAGTAT CTGGAGTAAT GCTGACACTA AAAACTCCAA CATGGGATTC
    TGGGATGATG CAGTGAAAGA GGTGGGACCT AGGAATTCTA CAAATAAAAA TAAAAACAAC
    GCCAGTCTCA GTAAATCTGT AGGTGTGTCT AACCGGCAGA ATAAGAAAGT AGAAGAAGAA
    GAAAAGTTGC TGAAGCTCTT TCAGGGAGTA AATAAAGCCC AAGATGGATT TACCCAGTGG
    TGTGAGCAGA TGCTTCATGC CCTTAATACG GCAAATAACT TGGATGTTCC CACTTTTGTT
    TCTTTCCTGA AAGAAGTAGA ATCTCCTTAT GAGGTCCATG ATTATATTAG GGCCTATTTA
    GGAGATACCT CTGAGGCCAA GGAGTTTGCC AAGCAGTTCC TCGAGCGCCG TGCCAAACAG
    AAAGCCAGCC AGCGGCAGCA GCAGCAGCAG CAACAACAGC AACAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GGACTCTGTG TGGGGAATGA ACCACAGTGC ACTCCATTCA
    GTATTTCAGA CCAATCAAAG CAACAACCAA CAATCCAATT TTGAGGCCGT ACAAAGTGGC
    AAGAAGAAGA AAAAGCAGAA GATGGTCCGA GCAGATCCCA GTTTGTTAGG ATTTTCAGTC
    AATGCTTCAT CAGAGCGACT CAATATGGGT GAAATCGAGA CTTTGGATGA CTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297280
    Clone ID Related Accession (Same CDS sequence) XM_022410015.1 , XM_022410016.1
    Accession Version XM_022410015.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGGA CTCTGTGTGG GGAATGAACC ACAGTGCACT CCATTCAGTA
    TTTCAGACCA ATCAAAGCAA CAACCAACAA TCCAATTTTG AGGCCGTACA AAGTGGCAAG
    AAGAAGAAAA AGCAGAAGAT GGTCCGAGCA GATCCCAGTT TGTTAGGATT TTCAGTCAAT
    GCTTCATCAG AGCGACTCAA TATGGGTGAA ATCGAGACTT TGGATGACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265723.1
    CDS215..4108
    Translation

    Target ORF information:

    RefSeq Version XM_022410015.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410015.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGGA CTCTGTGTGG GGAATGAACC ACAGTGCACT CCATTCAGTA
    TTTCAGACCA ATCAAAGCAA CAACCAACAA TCCAATTTTG AGGCCGTACA AAGTGGCAAG
    AAGAAGAAAA AGCAGAAGAT GGTCCGAGCA GATCCCAGTT TGTTAGGATT TTCAGTCAAT
    GCTTCATCAG AGCGACTCAA TATGGGTGAA ATCGAGACTT TGGATGACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297280
    Clone ID Related Accession (Same CDS sequence) XM_022410015.1 , XM_022410016.1
    Accession Version XM_022410016.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3894bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGGA CTCTGTGTGG GGAATGAACC ACAGTGCACT CCATTCAGTA
    TTTCAGACCA ATCAAAGCAA CAACCAACAA TCCAATTTTG AGGCCGTACA AAGTGGCAAG
    AAGAAGAAAA AGCAGAAGAT GGTCCGAGCA GATCCCAGTT TGTTAGGATT TTCAGTCAAT
    GCTTCATCAG AGCGACTCAA TATGGGTGAA ATCGAGACTT TGGATGACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265724.1
    CDS256..4149
    Translation

    Target ORF information:

    RefSeq Version XM_022410016.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410016.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGGA CTCTGTGTGG GGAATGAACC ACAGTGCACT CCATTCAGTA
    TTTCAGACCA ATCAAAGCAA CAACCAACAA TCCAATTTTG AGGCCGTACA AAGTGGCAAG
    AAGAAGAAAA AGCAGAAGAT GGTCCGAGCA GATCCCAGTT TGTTAGGATT TTCAGTCAAT
    GCTTCATCAG AGCGACTCAA TATGGGTGAA ATCGAGACTT TGGATGACTA CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297281
    Clone ID Related Accession (Same CDS sequence) XM_022410017.1
    Accession Version XM_022410017.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3891bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGCTAGTGA GGAAACATCC CAGACCTCAT CATCATCTGC AAGACCAGGT
    ACTCCTTCAG ACCATCAGCC TCAGGAAGCA CCGCAATTTG AGAGGAAGGA GGAATCCCAA
    ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG GGTCAGACGG AAAATAGCCT GCCAACCAAA
    GTGCCCAGCA GAGGGGATGA AATGGTTTCT GATGTCCAGC AGCCCCTGTC ACAGATTCCT
    TCAGATACAG CCTCTCCTCT TCTCATACTT CCACCGCCTG TTCCCAATCC TAGTCCTGCC
    CTCCGGCCAG TTGAAACGCC GGTCGTAGGT GCTCCTGGTA TGGGCAGTGT TTCCACAGAG
    CCAGATGATG AAGAGGGTCT CAAGCATTTG GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT
    CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA GCACAGAGCT
    AAGGGAGTCT CAATTCCATT GATGCACGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT
    CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGT
    TATTTTACTA TGTCTTTATT GGTGAAGAGA GCATGTGATG AAAGCTTCCA ACCTCTTGGT
    GATATCATGA AAATGTGGGG AAGGGTTCCC TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT
    ATGGGAGAGT TGGACCAGGA ACGACTGACC AGGCAGCAGG AGCTCACTGC CTTGTACCAA
    ATGCAGCACC TTCAGTACCA GCAGTTCTTA ATACAACAGC AGTATGCACA GGTTTTGGCT
    CAACAACAGA AAGCAGCTTT GTCTTCACAG CAGCAGCAGC AGTTGGCACT TCTTCTCCAA
    CAGTTCCAGG CCCTGAAAAT GAGAATATCT GATCAGAACA TCATTCCTTC AGTAACTAGG
    TCTGTGTCGG TGCCAGATAC TGGTTCTATC TGGGAGCTTC AACCAACAGC CTCACAGCCT
    ACAGTTTGGG AAGGTGGTAG TGTATGGGAT CTTCCCCTTG ACACCACAAC TCCAGGACCA
    GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA
    AGAGAGGCAG AAATGAGGGC AAAACGGGAA GAGGAGGAAC GAAAGAGGCA AGAAGAACTC
    CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG
    GAAGAAGAGC TCGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAAAG GGAGCAAGAA
    ATTGCATTAA GGCGACAGCG AGAAGAGGAA GAAAGACAAC AGCAAGAAGA AGCTCTTAGG
    AGACTGGAGG AGAGGAGGAG AGAAGAGGAA GAAAGGCGGA AGCAGGAGGA GTTGTTACGC
    AAGCAGGAAG AGGAAGCTGC AAAATGGGCC AGGGAAGAAG AAGAAGCCCA GCGTCGATTA
    GAGGAGAACC GGCTGCGAAT GGAAGAGGAG GCAGCCCGAC TCCGGCATGA AGAAGAGGAG
    CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG CAGAAGGAGT TAATGCGCCA GAGGCAGCAG
    CAACAAGAGG CTCTCCGGAG GTTGCAGCAG CAACAGCAGC AGCAGCAGCT GGCTCAGATG
    AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG CAGTCCAATA CAACAGCATG TCAGTCCCAG
    GCAACGCTGT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA
    GAGGAGCAAA GACGCCAGCA GAGGGAACTG ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGA AACTCTCTGG ATGGGGGAAT GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT
    CTCTTGGAGA TACAGCAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAACA GCAGCAGCAG
    CAGCACCAGC AACCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT
    GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC ATCTGACCTA
    GTCAGTAGTA TCTGGAGTAA TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT
    GCAGTGAAAG AGGTGGGACC TAGGAATTCT ACAAATAAAA ATAAAAACAA CGCCAGTCTC
    AGTAAATCTG TAGGTGTGTC TAACCGGCAG AATAAGAAAG TAGAAGAAGA AGAAAAGTTG
    CTGAAGCTCT TTCAGGGAGT AAATAAAGCC CAAGATGGAT TTACCCAGTG GTGTGAGCAG
    ATGCTTCATG CCCTTAATAC GGCAAATAAC TTGGATGTTC CCACTTTTGT TTCTTTCCTG
    AAAGAAGTAG AATCTCCTTA TGAGGTCCAT GATTATATTA GGGCCTATTT AGGAGATACC
    TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC CTCGAGCGCC GTGCCAAACA GAAAGCCAGC
    CAGCGGCAGC AGCAGCAGCA GCAACAACAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGACTC TGTGTGGGGA ATGAACCACA GTGCACTCCA TTCAGTATTT
    CAGACCAATC AAAGCAACAA CCAACAATCC AATTTTGAGG CCGTACAAAG TGGCAAGAAG
    AAGAAAAAGC AGAAGATGGT CCGAGCAGAT CCCAGTTTGT TAGGATTTTC AGTCAATGCT
    TCATCAGAGC GACTCAATAT GGGTGAAATC GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265725.1
    CDS215..4105
    Translation

    Target ORF information:

    RefSeq Version XM_022410017.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410017.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGCTAGTGA GGAAACATCC CAGACCTCAT CATCATCTGC AAGACCAGGT
    ACTCCTTCAG ACCATCAGCC TCAGGAAGCA CCGCAATTTG AGAGGAAGGA GGAATCCCAA
    ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG GGTCAGACGG AAAATAGCCT GCCAACCAAA
    GTGCCCAGCA GAGGGGATGA AATGGTTTCT GATGTCCAGC AGCCCCTGTC ACAGATTCCT
    TCAGATACAG CCTCTCCTCT TCTCATACTT CCACCGCCTG TTCCCAATCC TAGTCCTGCC
    CTCCGGCCAG TTGAAACGCC GGTCGTAGGT GCTCCTGGTA TGGGCAGTGT TTCCACAGAG
    CCAGATGATG AAGAGGGTCT CAAGCATTTG GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT
    CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA GCACAGAGCT
    AAGGGAGTCT CAATTCCATT GATGCACGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT
    CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGT
    TATTTTACTA TGTCTTTATT GGTGAAGAGA GCATGTGATG AAAGCTTCCA ACCTCTTGGT
    GATATCATGA AAATGTGGGG AAGGGTTCCC TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT
    ATGGGAGAGT TGGACCAGGA ACGACTGACC AGGCAGCAGG AGCTCACTGC CTTGTACCAA
    ATGCAGCACC TTCAGTACCA GCAGTTCTTA ATACAACAGC AGTATGCACA GGTTTTGGCT
    CAACAACAGA AAGCAGCTTT GTCTTCACAG CAGCAGCAGC AGTTGGCACT TCTTCTCCAA
    CAGTTCCAGG CCCTGAAAAT GAGAATATCT GATCAGAACA TCATTCCTTC AGTAACTAGG
    TCTGTGTCGG TGCCAGATAC TGGTTCTATC TGGGAGCTTC AACCAACAGC CTCACAGCCT
    ACAGTTTGGG AAGGTGGTAG TGTATGGGAT CTTCCCCTTG ACACCACAAC TCCAGGACCA
    GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA
    AGAGAGGCAG AAATGAGGGC AAAACGGGAA GAGGAGGAAC GAAAGAGGCA AGAAGAACTC
    CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG
    GAAGAAGAGC TCGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAAAG GGAGCAAGAA
    ATTGCATTAA GGCGACAGCG AGAAGAGGAA GAAAGACAAC AGCAAGAAGA AGCTCTTAGG
    AGACTGGAGG AGAGGAGGAG AGAAGAGGAA GAAAGGCGGA AGCAGGAGGA GTTGTTACGC
    AAGCAGGAAG AGGAAGCTGC AAAATGGGCC AGGGAAGAAG AAGAAGCCCA GCGTCGATTA
    GAGGAGAACC GGCTGCGAAT GGAAGAGGAG GCAGCCCGAC TCCGGCATGA AGAAGAGGAG
    CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG CAGAAGGAGT TAATGCGCCA GAGGCAGCAG
    CAACAAGAGG CTCTCCGGAG GTTGCAGCAG CAACAGCAGC AGCAGCAGCT GGCTCAGATG
    AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG CAGTCCAATA CAACAGCATG TCAGTCCCAG
    GCAACGCTGT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA
    GAGGAGCAAA GACGCCAGCA GAGGGAACTG ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGA AACTCTCTGG ATGGGGGAAT GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT
    CTCTTGGAGA TACAGCAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAACA GCAGCAGCAG
    CAGCACCAGC AACCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT
    GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC ATCTGACCTA
    GTCAGTAGTA TCTGGAGTAA TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT
    GCAGTGAAAG AGGTGGGACC TAGGAATTCT ACAAATAAAA ATAAAAACAA CGCCAGTCTC
    AGTAAATCTG TAGGTGTGTC TAACCGGCAG AATAAGAAAG TAGAAGAAGA AGAAAAGTTG
    CTGAAGCTCT TTCAGGGAGT AAATAAAGCC CAAGATGGAT TTACCCAGTG GTGTGAGCAG
    ATGCTTCATG CCCTTAATAC GGCAAATAAC TTGGATGTTC CCACTTTTGT TTCTTTCCTG
    AAAGAAGTAG AATCTCCTTA TGAGGTCCAT GATTATATTA GGGCCTATTT AGGAGATACC
    TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC CTCGAGCGCC GTGCCAAACA GAAAGCCAGC
    CAGCGGCAGC AGCAGCAGCA GCAACAACAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGGACTC TGTGTGGGGA ATGAACCACA GTGCACTCCA TTCAGTATTT
    CAGACCAATC AAAGCAACAA CCAACAATCC AATTTTGAGG CCGTACAAAG TGGCAAGAAG
    AAGAAAAAGC AGAAGATGGT CCGAGCAGAT CCCAGTTTGT TAGGATTTTC AGTCAATGCT
    TCATCAGAGC GACTCAATAT GGGTGAAATC GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297282
    Clone ID Related Accession (Same CDS sequence) XM_022410018.1
    Accession Version XM_022410018.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGGACTCTGT GTGGGGAATG AACCACAGTG CACTCCATTC AGTATTTCAG
    ACCAATCAAA GCAACAACCA ACAATCCAAT TTTGAGGCCG TACAAAGTGG CAAGAAGAAG
    AAAAAGCAGA AGATGGTCCG AGCAGATCCC AGTTTGTTAG GATTTTCAGT CAATGCTTCA
    TCAGAGCGAC TCAATATGGG TGAAATCGAG ACTTTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265726.1
    CDS215..4102
    Translation

    Target ORF information:

    RefSeq Version XM_022410018.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410018.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGGACTCTGT GTGGGGAATG AACCACAGTG CACTCCATTC AGTATTTCAG
    ACCAATCAAA GCAACAACCA ACAATCCAAT TTTGAGGCCG TACAAAGTGG CAAGAAGAAG
    AAAAAGCAGA AGATGGTCCG AGCAGATCCC AGTTTGTTAG GATTTTCAGT CAATGCTTCA
    TCAGAGCGAC TCAATATGGG TGAAATCGAG ACTTTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297283
    Clone ID Related Accession (Same CDS sequence) XM_022410019.1
    Accession Version XM_022410019.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3888bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGCTAGTGA GGAAACATCC CAGACCTCAT CATCATCTGC AAGACCAGGT
    ACTCCTTCAG ACCATCAGCC TCAGGAAGCA CCGCAATTTG AGAGGAAGGA GGAATCCCAA
    ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG GGTCAGACGG AAAATAGCCT GCCAACCAAA
    GTGCCCAGCA GAGGGGATGA AATGGTTTCT GATGTCCAGC AGCCCCTGTC ACAGATTCCT
    TCAGATACAG CCTCTCCTCT TCTCATACTT CCACCGCCTG TTCCCAATCC TAGTCCTGCC
    CTCCGGCCAG TTGAAACGCC GGTCGTAGGT GCTCCTGGTA TGGGCAGTGT TTCCACAGAG
    CCAGATGATG AAGAGGGTCT CAAGCATTTG GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT
    CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA GCACAGAGCT
    AAGGGAGTCT CAATTCCATT GATGCACGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT
    CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGT
    TATTTTACTA TGTCTTTATT GGTGAAGAGA GCATGTGATG AAAGCTTCCA ACCTCTTGGT
    GATATCATGA AAATGTGGGG AAGGGTTCCC TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT
    ATGGGAGAGT TGGACCAGGA ACGACTGACC AGGCAGCAGG AGCTCACTGC CTTGTACCAA
    ATGCAGCACC TTCAGTACCA GCAGTTCTTA ATACAACAGC AGTATGCACA GGTTTTGGCT
    CAACAACAGA AAGCAGCTTT GTCTTCACAG CAGCAGCAGC AGTTGGCACT TCTTCTCCAA
    CAGTTCCAGG CCCTGAAAAT GAGAATATCT GATCAGAACA TCATTCCTTC AGTAACTAGG
    TCTGTGTCGG TGCCAGATAC TGGTTCTATC TGGGAGCTTC AACCAACAGC CTCACAGCCT
    ACAGTTTGGG AAGGTGGTAG TGTATGGGAT CTTCCCCTTG ACACCACAAC TCCAGGACCA
    GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA
    AGAGAGGCAG AAATGAGGGC AAAACGGGAA GAGGAGGAAC GAAAGAGGCA AGAAGAACTC
    CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG
    GAAGAAGAGC TCGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAAAG GGAGCAAGAA
    ATTGCATTAA GGCGACAGCG AGAAGAGGAA GAAAGACAAC AGCAAGAAGA AGCTCTTAGG
    AGACTGGAGG AGAGGAGGAG AGAAGAGGAA GAAAGGCGGA AGCAGGAGGA GTTGTTACGC
    AAGCAGGAAG AGGAAGCTGC AAAATGGGCC AGGGAAGAAG AAGAAGCCCA GCGTCGATTA
    GAGGAGAACC GGCTGCGAAT GGAAGAGGAG GCAGCCCGAC TCCGGCATGA AGAAGAGGAG
    CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG CAGAAGGAGT TAATGCGCCA GAGGCAGCAG
    CAACAAGAGG CTCTCCGGAG GTTGCAGCAG CAACAGCAGC AGCAGCAGCT GGCTCAGATG
    AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG CAGTCCAATA CAACAGCATG TCAGTCCCAG
    GCAACGCTGT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA
    GAGGAGCAAA GACGCCAGCA GAGGGAACTG ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGA AACTCTCTGG ATGGGGGAAT GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT
    CTCTTGGAGA TACAGCAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAACA GCAGCAGCAG
    CAGCACCAGC AACCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT
    GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC ATCTGACCTA
    GTCAGTAGTA TCTGGAGTAA TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT
    GCAGTGAAAG AGGTGGGACC TAGGAATTCT ACAAATAAAA ATAAAAACAA CGCCAGTCTC
    AGTAAATCTG TAGGTGTGTC TAACCGGCAG AATAAGAAAG TAGAAGAAGA AGAAAAGTTG
    CTGAAGCTCT TTCAGGGAGT AAATAAAGCC CAAGATGGAT TTACCCAGTG GTGTGAGCAG
    ATGCTTCATG CCCTTAATAC GGCAAATAAC TTGGATGTTC CCACTTTTGT TTCTTTCCTG
    AAAGAAGTAG AATCTCCTTA TGAGGTCCAT GATTATATTA GGGCCTATTT AGGAGATACC
    TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC CTCGAGCGCC GTGCCAAACA GAAAGCCAGC
    CAGCGGCAGC AGCAGCAGCA GCAACAACAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGGACTCTGT GTGGGGAATG AACCACAGTG CACTCCATTC AGTATTTCAG
    ACCAATCAAA GCAACAACCA ACAATCCAAT TTTGAGGCCG TACAAAGTGG CAAGAAGAAG
    AAAAAGCAGA AGATGGTCCG AGCAGATCCC AGTTTGTTAG GATTTTCAGT CAATGCTTCA
    TCAGAGCGAC TCAATATGGG TGAAATCGAG ACTTTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265727.1
    CDS215..4102
    Translation

    Target ORF information:

    RefSeq Version XM_022410019.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410019.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGCTAGTGA GGAAACATCC CAGACCTCAT CATCATCTGC AAGACCAGGT
    ACTCCTTCAG ACCATCAGCC TCAGGAAGCA CCGCAATTTG AGAGGAAGGA GGAATCCCAA
    ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG GGTCAGACGG AAAATAGCCT GCCAACCAAA
    GTGCCCAGCA GAGGGGATGA AATGGTTTCT GATGTCCAGC AGCCCCTGTC ACAGATTCCT
    TCAGATACAG CCTCTCCTCT TCTCATACTT CCACCGCCTG TTCCCAATCC TAGTCCTGCC
    CTCCGGCCAG TTGAAACGCC GGTCGTAGGT GCTCCTGGTA TGGGCAGTGT TTCCACAGAG
    CCAGATGATG AAGAGGGTCT CAAGCATTTG GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT
    CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA GCACAGAGCT
    AAGGGAGTCT CAATTCCATT GATGCACGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT
    CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGT
    TATTTTACTA TGTCTTTATT GGTGAAGAGA GCATGTGATG AAAGCTTCCA ACCTCTTGGT
    GATATCATGA AAATGTGGGG AAGGGTTCCC TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT
    ATGGGAGAGT TGGACCAGGA ACGACTGACC AGGCAGCAGG AGCTCACTGC CTTGTACCAA
    ATGCAGCACC TTCAGTACCA GCAGTTCTTA ATACAACAGC AGTATGCACA GGTTTTGGCT
    CAACAACAGA AAGCAGCTTT GTCTTCACAG CAGCAGCAGC AGTTGGCACT TCTTCTCCAA
    CAGTTCCAGG CCCTGAAAAT GAGAATATCT GATCAGAACA TCATTCCTTC AGTAACTAGG
    TCTGTGTCGG TGCCAGATAC TGGTTCTATC TGGGAGCTTC AACCAACAGC CTCACAGCCT
    ACAGTTTGGG AAGGTGGTAG TGTATGGGAT CTTCCCCTTG ACACCACAAC TCCAGGACCA
    GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA
    AGAGAGGCAG AAATGAGGGC AAAACGGGAA GAGGAGGAAC GAAAGAGGCA AGAAGAACTC
    CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG
    GAAGAAGAGC TCGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAAAG GGAGCAAGAA
    ATTGCATTAA GGCGACAGCG AGAAGAGGAA GAAAGACAAC AGCAAGAAGA AGCTCTTAGG
    AGACTGGAGG AGAGGAGGAG AGAAGAGGAA GAAAGGCGGA AGCAGGAGGA GTTGTTACGC
    AAGCAGGAAG AGGAAGCTGC AAAATGGGCC AGGGAAGAAG AAGAAGCCCA GCGTCGATTA
    GAGGAGAACC GGCTGCGAAT GGAAGAGGAG GCAGCCCGAC TCCGGCATGA AGAAGAGGAG
    CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG CAGAAGGAGT TAATGCGCCA GAGGCAGCAG
    CAACAAGAGG CTCTCCGGAG GTTGCAGCAG CAACAGCAGC AGCAGCAGCT GGCTCAGATG
    AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG CAGTCCAATA CAACAGCATG TCAGTCCCAG
    GCAACGCTGT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA
    GAGGAGCAAA GACGCCAGCA GAGGGAACTG ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGA AACTCTCTGG ATGGGGGAAT GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT
    CTCTTGGAGA TACAGCAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAACA GCAGCAGCAG
    CAGCACCAGC AACCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT
    GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC ATCTGACCTA
    GTCAGTAGTA TCTGGAGTAA TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT
    GCAGTGAAAG AGGTGGGACC TAGGAATTCT ACAAATAAAA ATAAAAACAA CGCCAGTCTC
    AGTAAATCTG TAGGTGTGTC TAACCGGCAG AATAAGAAAG TAGAAGAAGA AGAAAAGTTG
    CTGAAGCTCT TTCAGGGAGT AAATAAAGCC CAAGATGGAT TTACCCAGTG GTGTGAGCAG
    ATGCTTCATG CCCTTAATAC GGCAAATAAC TTGGATGTTC CCACTTTTGT TTCTTTCCTG
    AAAGAAGTAG AATCTCCTTA TGAGGTCCAT GATTATATTA GGGCCTATTT AGGAGATACC
    TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC CTCGAGCGCC GTGCCAAACA GAAAGCCAGC
    CAGCGGCAGC AGCAGCAGCA GCAACAACAG CAACAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGGACTCTGT GTGGGGAATG AACCACAGTG CACTCCATTC AGTATTTCAG
    ACCAATCAAA GCAACAACCA ACAATCCAAT TTTGAGGCCG TACAAAGTGG CAAGAAGAAG
    AAAAAGCAGA AGATGGTCCG AGCAGATCCC AGTTTGTTAG GATTTTCAGT CAATGCTTCA
    TCAGAGCGAC TCAATATGGG TGAAATCGAG ACTTTGGATG ACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297284
    Clone ID Related Accession (Same CDS sequence) XM_022410020.1
    Accession Version XM_022410020.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3885bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGG ACTCTGTGTG GGGAATGAAC CACAGTGCAC TCCATTCAGT ATTTCAGACC
    AATCAAAGCA ACAACCAACA ATCCAATTTT GAGGCCGTAC AAAGTGGCAA GAAGAAGAAA
    AAGCAGAAGA TGGTCCGAGC AGATCCCAGT TTGTTAGGAT TTTCAGTCAA TGCTTCATCA
    GAGCGACTCA ATATGGGTGA AATCGAGACT TTGGATGACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265728.1
    CDS215..4099
    Translation

    Target ORF information:

    RefSeq Version XM_022410020.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410020.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGG ACTCTGTGTG GGGAATGAAC CACAGTGCAC TCCATTCAGT ATTTCAGACC
    AATCAAAGCA ACAACCAACA ATCCAATTTT GAGGCCGTAC AAAGTGGCAA GAAGAAGAAA
    AAGCAGAAGA TGGTCCGAGC AGATCCCAGT TTGTTAGGAT TTTCAGTCAA TGCTTCATCA
    GAGCGACTCA ATATGGGTGA AATCGAGACT TTGGATGACT ACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297285
    Clone ID Related Accession (Same CDS sequence) XM_022410021.1
    Accession Version XM_022410021.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3876bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGGCTGGCGA
    GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG
    GGTTATCGAA GGGTGCGCTC TGGCAGTGGA AGCATAGACG ATGACCGGGA TAGTTTGCCC
    GAGTGGTGCT TAGAGGACGC TGAAGAAGAG ATGGGCACAT TTGACTCCTC TGGAGCATTC
    CTCTTTCTAA AGAAAGTACA GAAAGAGCCA ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGAGG AAGGTGGGGA GTGCTCTGAC TCTGAGAGCA GCCATAATGA AGAAGCCAAA
    GAACCTGATA AGACAAATAG GAAAGAGGGA GAGAAGACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CATCCCAGAC CTCATCATCA TCTGCAAGAC CAGGTACTCC TTCAGACCAT
    CAGCCTCAGG AAGCACCGCA ATTTGAGAGG AAGGAGGAAT CCCAAACTGA GCAAACGGAA
    AAAGCTGAGG AAGAGGGTCA GACGGAAAAT AGCCTGCCAA CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTTCTGATGT CCAGCAGCCC CTGTCACAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC GCCTGTTCCC AATCCTAGTC CTGCCCTCCG GCCAGTTGAA
    ACGCCGGTCG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAGCCAGA TGATGAAGAG
    GGTCTCAAGC ATTTGGAGCA GCAAGCTGAG AAGATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AGAGACTGGC ATCAAAACTG CAAGAGCACA GAGCTAAGGG AGTCTCAATT
    CCATTGATGC ACGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCGGAA TGGTTTCAGG CGGGTTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCATG TGATGAAAGC TTCCAACCTC TTGGTGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTCTC TCCCGGCCCA GCTCCCCCTC CTCATATGGG AGAGTTGGAC
    CAGGAACGAC TGACCAGGCA GCAGGAGCTC ACTGCCTTGT ACCAAATGCA GCACCTTCAG
    TACCAGCAGT TCTTAATACA ACAGCAGTAT GCACAGGTTT TGGCTCAACA ACAGAAAGCA
    GCTTTGTCTT CACAGCAGCA GCAGCAGTTG GCACTTCTTC TCCAACAGTT CCAGGCCCTG
    AAAATGAGAA TATCTGATCA GAACATCATT CCTTCAGTAA CTAGGTCTGT GTCGGTGCCA
    GATACTGGTT CTATCTGGGA GCTTCAACCA ACAGCCTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC CCTTGACACC ACAACTCCAG GACCAGCCCT TGAACAGCTT
    CAGCAGATGG AGAAGGCCAA AGCTGCAAAG CTAGAACAGG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA GGAACGAAAG AGGCAAGAAG AACTCCGGAG ACAACAGGAG
    GAAATTCTTC GGAGACAGCA GGAGGAGGAA AGGAAGAGGC GGGAGGAAGA AGAGCTCGCC
    CGAAGGAAAC AGGAAGAAGC TCTGCGACGC CAAAGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAACAGCAA GAAGAAGCTC TTAGGAGACT GGAGGAGAGG
    AGGAGAGAAG AGGAAGAAAG GCGGAAGCAG GAGGAGTTGT TACGCAAGCA GGAAGAGGAA
    GCTGCAAAAT GGGCCAGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGAATGGAAG AGGAGGCAGC CCGACTCCGG CATGAAGAAG AGGAGCGGAA GAGGAAAGAG
    TTAGAGCTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAACA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAACA GCAGCAGCAG CAGCTGGCTC AGATGAAGCT TCCCTCTTCT
    TCCACATGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCAAC GCTGTCATTG
    GCTGAAATCC AAAAACTAGA AGAGGAACGA GAACGGCAGC TTCGAGAGGA GCAAAGACGC
    CAGCAGAGGG AACTGATGAA AGCTCTGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAACTC
    TCTGGATGGG GGAATGTCAG TAAACCTGCA GGCACTGCGA AGTCTCTCTT GGAGATACAG
    CAGGAAGAAG CCAGGCAGAT GCAGAAGCAG CAACAGCAGC AGCAGCAGCA CCAGCAACCA
    AACAGAGCCC GGAATAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC CCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATCTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCTACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACC CAGTGGTGTG AGCAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACT TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATTAGGGCC TATTTAGGAG ATACCTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTCGA GCGCCGTGCC AAACAGAAAG CCAGCCAGCG GCAGCAGCAG
    CAGCAGCAAC AACAGCAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    GACTCTGTGT GGGGAATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAAAGC
    AACAACCAAC AATCCAATTT TGAGGCCGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG
    ATGGTCCGAG CAGATCCCAG TTTGTTAGGA TTTTCAGTCA ATGCTTCATC AGAGCGACTC
    AATATGGGTG AAATCGAGAC TTTGGATGAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265729.1
    CDS215..4090
    Translation

    Target ORF information:

    RefSeq Version XM_022410021.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410021.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGGCTGGCGA
    GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG
    GGTTATCGAA GGGTGCGCTC TGGCAGTGGA AGCATAGACG ATGACCGGGA TAGTTTGCCC
    GAGTGGTGCT TAGAGGACGC TGAAGAAGAG ATGGGCACAT TTGACTCCTC TGGAGCATTC
    CTCTTTCTAA AGAAAGTACA GAAAGAGCCA ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGAGG AAGGTGGGGA GTGCTCTGAC TCTGAGAGCA GCCATAATGA AGAAGCCAAA
    GAACCTGATA AGACAAATAG GAAAGAGGGA GAGAAGACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CATCCCAGAC CTCATCATCA TCTGCAAGAC CAGGTACTCC TTCAGACCAT
    CAGCCTCAGG AAGCACCGCA ATTTGAGAGG AAGGAGGAAT CCCAAACTGA GCAAACGGAA
    AAAGCTGAGG AAGAGGGTCA GACGGAAAAT AGCCTGCCAA CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTTCTGATGT CCAGCAGCCC CTGTCACAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC GCCTGTTCCC AATCCTAGTC CTGCCCTCCG GCCAGTTGAA
    ACGCCGGTCG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAGCCAGA TGATGAAGAG
    GGTCTCAAGC ATTTGGAGCA GCAAGCTGAG AAGATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AGAGACTGGC ATCAAAACTG CAAGAGCACA GAGCTAAGGG AGTCTCAATT
    CCATTGATGC ACGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCGGAA TGGTTTCAGG CGGGTTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCATG TGATGAAAGC TTCCAACCTC TTGGTGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTCTC TCCCGGCCCA GCTCCCCCTC CTCATATGGG AGAGTTGGAC
    CAGGAACGAC TGACCAGGCA GCAGGAGCTC ACTGCCTTGT ACCAAATGCA GCACCTTCAG
    TACCAGCAGT TCTTAATACA ACAGCAGTAT GCACAGGTTT TGGCTCAACA ACAGAAAGCA
    GCTTTGTCTT CACAGCAGCA GCAGCAGTTG GCACTTCTTC TCCAACAGTT CCAGGCCCTG
    AAAATGAGAA TATCTGATCA GAACATCATT CCTTCAGTAA CTAGGTCTGT GTCGGTGCCA
    GATACTGGTT CTATCTGGGA GCTTCAACCA ACAGCCTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC CCTTGACACC ACAACTCCAG GACCAGCCCT TGAACAGCTT
    CAGCAGATGG AGAAGGCCAA AGCTGCAAAG CTAGAACAGG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA GGAACGAAAG AGGCAAGAAG AACTCCGGAG ACAACAGGAG
    GAAATTCTTC GGAGACAGCA GGAGGAGGAA AGGAAGAGGC GGGAGGAAGA AGAGCTCGCC
    CGAAGGAAAC AGGAAGAAGC TCTGCGACGC CAAAGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAACAGCAA GAAGAAGCTC TTAGGAGACT GGAGGAGAGG
    AGGAGAGAAG AGGAAGAAAG GCGGAAGCAG GAGGAGTTGT TACGCAAGCA GGAAGAGGAA
    GCTGCAAAAT GGGCCAGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGAATGGAAG AGGAGGCAGC CCGACTCCGG CATGAAGAAG AGGAGCGGAA GAGGAAAGAG
    TTAGAGCTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAACA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAACA GCAGCAGCAG CAGCTGGCTC AGATGAAGCT TCCCTCTTCT
    TCCACATGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCAAC GCTGTCATTG
    GCTGAAATCC AAAAACTAGA AGAGGAACGA GAACGGCAGC TTCGAGAGGA GCAAAGACGC
    CAGCAGAGGG AACTGATGAA AGCTCTGCAG CAGCAGCAGC AGCAGCAGCA GCAGAAACTC
    TCTGGATGGG GGAATGTCAG TAAACCTGCA GGCACTGCGA AGTCTCTCTT GGAGATACAG
    CAGGAAGAAG CCAGGCAGAT GCAGAAGCAG CAACAGCAGC AGCAGCAGCA CCAGCAACCA
    AACAGAGCCC GGAATAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC CCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATCTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCTACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACC CAGTGGTGTG AGCAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACT TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATTAGGGCC TATTTAGGAG ATACCTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTCGA GCGCCGTGCC AAACAGAAAG CCAGCCAGCG GCAGCAGCAG
    CAGCAGCAAC AACAGCAACA GCAGCAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    GACTCTGTGT GGGGAATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAAAGC
    AACAACCAAC AATCCAATTT TGAGGCCGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG
    ATGGTCCGAG CAGATCCCAG TTTGTTAGGA TTTTCAGTCA ATGCTTCATC AGAGCGACTC
    AATATGGGTG AAATCGAGAC TTTGGATGAC TACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297286
    Clone ID Related Accession (Same CDS sequence) XM_022410022.1
    Accession Version XM_022410022.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3741bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG GAGACCAAAT TTTGAGGAAG GTGGACCAAC ATCGGTGGGA
    AGAAAGCATG AGTTTATACG ATCAGAAAGT GAAAACTGGC GTATTTTTAG AGAAGAACAA
    AACGGAGAAG ATGAAGATGG AGGTTGGAGA CTAGCTGGAC CGAGACGGGA TGGAGAGAGG
    TGGCGTCCCC ATAGCCCTGA TGGCCCTCGT TCTGCAGGCT GGCGAGAACA CATGGAACGA
    CGGCGAAGGT TTGAGTTTGA TTTTCGAGAT CGGGATGATG AACGGGGTTA TCGAAGGGTG
    CGCTCTGGCA GTGGAAGCAT AGACGATGAC CGGGATAGTT TGCCCGAGTG GTGCTTAGAG
    GACGCTGAAG AAGAGATGGG CACATTTGAC TCCTCTGGAG CATTCCTCTT TCTAAAGAAA
    GTACAGAAAG AGCCAATTCC AGAAGAGCAG GAGATGGACT TCCGGCCTGT GGAGGAAGGT
    GGGGAGTGCT CTGACTCTGA GAGCAGCCAT AATGAAGAAG CCAAAGAACC TGATAAGACA
    AATAGGAAAG AGGGAGAGAA GACAGATAGA GTAGGAGTTG AAGCTAGTGA GGAAACATCC
    CAGACCTCAT CATCATCTGC AAGACCAGGT ACTCCTTCAG ACCATCAGCC TCAGGAAGCA
    CCGCAATTTG AGAGGAAGGA GGAATCCCAA ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG
    GGTCAGACGG AAAATAGCCT GCCAACCAAA GTGCCCAGCA GAGGGGATGA AATGGTTTCT
    GATGTCCAGC AGCCCCTGTC ACAGATTCCT TCAGATACAG CCTCTCCTCT TCTCATACTT
    CCACCGCCTG TTCCCAATCC TAGTCCTGCC CTCCGGCCAG TTGAAACGCC GGTCGTAGGT
    GCTCCTGGTA TGGGCAGTGT TTCCACAGAG CCAGATGATG AAGAGGGTCT CAAGCATTTG
    GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT CTCCAAGACA GTGCACTAGA TGATGAGAGA
    CTGGCATCAA AACTGCAAGA GCACAGAGCT AAGGGAGTCT CAATTCCATT GATGCACGAA
    GCAATGCAGA AGTGGTATTA CAAAGATCCT CAGGGAGAAA TTCAAGGTCC CTTCAATAAT
    CAGGAGATGG CGGAATGGTT TCAGGCGGGT TATTTTACTA TGTCTTTATT GGTGAAGAGA
    GCATGTGATG AAAGCTTCCA ACCTCTTGGT GATATCATGA AAATGTGGGG AAGGGTTCCC
    TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT ATGGGAGAGT TGGACCAGGA ACGACTGACC
    AGGCAGCAGG AGCTCACTGC CTTGTACCAA ATGCAGCACC TTCAGTACCA GCAGTTCTTA
    ATACAACAGC AGTATGCACA GGTTTTGGCT CAACAACAGA AAGCAGCTTT GTCTTCACAG
    CAGCAGCAGC AGTTGGCACT TCTTCTCCAA CAGTTCCAGG CCCTGAAAAT GAGAATATCT
    GATCAGAACA TCATTCCTTC AGTAACTAGG TCTGTGTCGG TGCCAGATAC TGGTTCTATC
    TGGGAGCTTC AACCAACAGC CTCACAGCCT ACAGTTTGGG AAGGTGGTAG TGTATGGGAT
    CTTCCCCTTG ACACCACAAC TCCAGGACCA GCCCTTGAAC AGCTTCAGCA GATGGAGAAG
    GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA AGAGAGGCAG AAATGAGGGC AAAACGGGAA
    GAGGAGGAAC GAAAGAGGCA AGAAGAACTC CGGAGACAAC AGGAGGAAAT TCTTCGGAGA
    CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG GAAGAAGAGC TCGCCCGAAG GAAACAGGAA
    GAAGCTCTGC GACGCCAAAG GGAGCAAGAA ATTGCATTAA GGCGACAGCG AGAAGAGGAA
    GAAAGACAAC AGCAAGAAGA AGCTCTTAGG AGACTGGAGG AGAGGAGGAG AGAAGAGGAA
    GAAAGGCGGA AGCAGGAGGA GTTGTTACGC AAGCAGGAAG AGGAAGCTGC AAAATGGGCC
    AGGGAAGAAG AAGAAGCCCA GCGTCGATTA GAGGAGAACC GGCTGCGAAT GGAAGAGGAG
    GCAGCCCGAC TCCGGCATGA AGAAGAGGAG CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG
    CAGAAGGAGT TAATGCGCCA GAGGCAGCAG CAACAAGAGG CTCTCCGGAG GTTGCAGCAG
    CAACAGCAGC AGCAGCAGCT GGCTCAGATG AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG
    CAGTCCAATA CAACAGCATG TCAGTCCCAG GCAACGCTGT CATTGGCTGA AATCCAAAAA
    CTAGAAGAGG AACGAGAACG GCAGCTTCGA GAGGAGCAAA GACGCCAGCA GAGGGAACTG
    ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG CAGCAGCAGA AACTCTCTGG ATGGGGGAAT
    GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT CTCTTGGAGA TACAGCAGGA AGAAGCCAGG
    CAGATGCAGA AGCAGCAACA GCAGCAGCAG CAGCACCAGC AACCAAACAG AGCCCGGAAT
    AATACGCATT CCAACCTGCA CACCAGCATT GGGAATTCTG TTTGGGGCTC TATAAATACT
    GGTCCCCCTA ACCAGTGGGC ATCTGACCTA GTCAGTAGTA TCTGGAGTAA TGCTGACACT
    AAAAACTCCA ACATGGGATT CTGGGATGAT GCAGTGAAAG AGGTGGGACC TAGGAATTCT
    ACAAATAAAA ATAAAAACAA CGCCAGTCTC AGTAAATCTG TAGGTGTGTC TAACCGGCAG
    AATAAGAAAG TAGAAGAAGA AGAAAAGTTG CTGAAGCTCT TTCAGGGAGT AAATAAAGCC
    CAAGATGGAT TTACCCAGTG GTGTGAGCAG ATGCTTCATG CCCTTAATAC GGCAAATAAC
    TTGGATGTTC CCACTTTTGT TTCTTTCCTG AAAGAAGTAG AATCTCCTTA TGAGGTCCAT
    GATTATATTA GGGCCTATTT AGGAGATACC TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC
    CTCGAGCGCC GTGCCAAACA GAAAGCCAGC CAGCGGCAGC AGCAGCAGCA GCAACAACAG
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGGACTC TGTGTGGGGA
    ATGAACCACA GTGCACTCCA TTCAGTATTT CAGACCAATC AAAGCAACAA CCAACAATCC
    AATTTTGAGG CCGTACAAAG TGGCAAGAAG AAGAAAAAGC AGAAGATGGT CCGAGCAGAT
    CCCAGTTTGT TAGGATTTTC AGTCAATGCT TCATCAGAGC GACTCAATAT GGGTGAAATC
    GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265730.1
    CDS215..3955
    Translation

    Target ORF information:

    RefSeq Version XM_022410022.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410022.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG GAGACCAAAT TTTGAGGAAG GTGGACCAAC ATCGGTGGGA
    AGAAAGCATG AGTTTATACG ATCAGAAAGT GAAAACTGGC GTATTTTTAG AGAAGAACAA
    AACGGAGAAG ATGAAGATGG AGGTTGGAGA CTAGCTGGAC CGAGACGGGA TGGAGAGAGG
    TGGCGTCCCC ATAGCCCTGA TGGCCCTCGT TCTGCAGGCT GGCGAGAACA CATGGAACGA
    CGGCGAAGGT TTGAGTTTGA TTTTCGAGAT CGGGATGATG AACGGGGTTA TCGAAGGGTG
    CGCTCTGGCA GTGGAAGCAT AGACGATGAC CGGGATAGTT TGCCCGAGTG GTGCTTAGAG
    GACGCTGAAG AAGAGATGGG CACATTTGAC TCCTCTGGAG CATTCCTCTT TCTAAAGAAA
    GTACAGAAAG AGCCAATTCC AGAAGAGCAG GAGATGGACT TCCGGCCTGT GGAGGAAGGT
    GGGGAGTGCT CTGACTCTGA GAGCAGCCAT AATGAAGAAG CCAAAGAACC TGATAAGACA
    AATAGGAAAG AGGGAGAGAA GACAGATAGA GTAGGAGTTG AAGCTAGTGA GGAAACATCC
    CAGACCTCAT CATCATCTGC AAGACCAGGT ACTCCTTCAG ACCATCAGCC TCAGGAAGCA
    CCGCAATTTG AGAGGAAGGA GGAATCCCAA ACTGAGCAAA CGGAAAAAGC TGAGGAAGAG
    GGTCAGACGG AAAATAGCCT GCCAACCAAA GTGCCCAGCA GAGGGGATGA AATGGTTTCT
    GATGTCCAGC AGCCCCTGTC ACAGATTCCT TCAGATACAG CCTCTCCTCT TCTCATACTT
    CCACCGCCTG TTCCCAATCC TAGTCCTGCC CTCCGGCCAG TTGAAACGCC GGTCGTAGGT
    GCTCCTGGTA TGGGCAGTGT TTCCACAGAG CCAGATGATG AAGAGGGTCT CAAGCATTTG
    GAGCAGCAAG CTGAGAAGAT GGTGGCTTAT CTCCAAGACA GTGCACTAGA TGATGAGAGA
    CTGGCATCAA AACTGCAAGA GCACAGAGCT AAGGGAGTCT CAATTCCATT GATGCACGAA
    GCAATGCAGA AGTGGTATTA CAAAGATCCT CAGGGAGAAA TTCAAGGTCC CTTCAATAAT
    CAGGAGATGG CGGAATGGTT TCAGGCGGGT TATTTTACTA TGTCTTTATT GGTGAAGAGA
    GCATGTGATG AAAGCTTCCA ACCTCTTGGT GATATCATGA AAATGTGGGG AAGGGTTCCC
    TTCTCTCCCG GCCCAGCTCC CCCTCCTCAT ATGGGAGAGT TGGACCAGGA ACGACTGACC
    AGGCAGCAGG AGCTCACTGC CTTGTACCAA ATGCAGCACC TTCAGTACCA GCAGTTCTTA
    ATACAACAGC AGTATGCACA GGTTTTGGCT CAACAACAGA AAGCAGCTTT GTCTTCACAG
    CAGCAGCAGC AGTTGGCACT TCTTCTCCAA CAGTTCCAGG CCCTGAAAAT GAGAATATCT
    GATCAGAACA TCATTCCTTC AGTAACTAGG TCTGTGTCGG TGCCAGATAC TGGTTCTATC
    TGGGAGCTTC AACCAACAGC CTCACAGCCT ACAGTTTGGG AAGGTGGTAG TGTATGGGAT
    CTTCCCCTTG ACACCACAAC TCCAGGACCA GCCCTTGAAC AGCTTCAGCA GATGGAGAAG
    GCCAAAGCTG CAAAGCTAGA ACAGGAGAGA AGAGAGGCAG AAATGAGGGC AAAACGGGAA
    GAGGAGGAAC GAAAGAGGCA AGAAGAACTC CGGAGACAAC AGGAGGAAAT TCTTCGGAGA
    CAGCAGGAGG AGGAAAGGAA GAGGCGGGAG GAAGAAGAGC TCGCCCGAAG GAAACAGGAA
    GAAGCTCTGC GACGCCAAAG GGAGCAAGAA ATTGCATTAA GGCGACAGCG AGAAGAGGAA
    GAAAGACAAC AGCAAGAAGA AGCTCTTAGG AGACTGGAGG AGAGGAGGAG AGAAGAGGAA
    GAAAGGCGGA AGCAGGAGGA GTTGTTACGC AAGCAGGAAG AGGAAGCTGC AAAATGGGCC
    AGGGAAGAAG AAGAAGCCCA GCGTCGATTA GAGGAGAACC GGCTGCGAAT GGAAGAGGAG
    GCAGCCCGAC TCCGGCATGA AGAAGAGGAG CGGAAGAGGA AAGAGTTAGA GCTCCAGCGG
    CAGAAGGAGT TAATGCGCCA GAGGCAGCAG CAACAAGAGG CTCTCCGGAG GTTGCAGCAG
    CAACAGCAGC AGCAGCAGCT GGCTCAGATG AAGCTTCCCT CTTCTTCCAC ATGGGGCCAG
    CAGTCCAATA CAACAGCATG TCAGTCCCAG GCAACGCTGT CATTGGCTGA AATCCAAAAA
    CTAGAAGAGG AACGAGAACG GCAGCTTCGA GAGGAGCAAA GACGCCAGCA GAGGGAACTG
    ATGAAAGCTC TGCAGCAGCA GCAGCAGCAG CAGCAGCAGA AACTCTCTGG ATGGGGGAAT
    GTCAGTAAAC CTGCAGGCAC TGCGAAGTCT CTCTTGGAGA TACAGCAGGA AGAAGCCAGG
    CAGATGCAGA AGCAGCAACA GCAGCAGCAG CAGCACCAGC AACCAAACAG AGCCCGGAAT
    AATACGCATT CCAACCTGCA CACCAGCATT GGGAATTCTG TTTGGGGCTC TATAAATACT
    GGTCCCCCTA ACCAGTGGGC ATCTGACCTA GTCAGTAGTA TCTGGAGTAA TGCTGACACT
    AAAAACTCCA ACATGGGATT CTGGGATGAT GCAGTGAAAG AGGTGGGACC TAGGAATTCT
    ACAAATAAAA ATAAAAACAA CGCCAGTCTC AGTAAATCTG TAGGTGTGTC TAACCGGCAG
    AATAAGAAAG TAGAAGAAGA AGAAAAGTTG CTGAAGCTCT TTCAGGGAGT AAATAAAGCC
    CAAGATGGAT TTACCCAGTG GTGTGAGCAG ATGCTTCATG CCCTTAATAC GGCAAATAAC
    TTGGATGTTC CCACTTTTGT TTCTTTCCTG AAAGAAGTAG AATCTCCTTA TGAGGTCCAT
    GATTATATTA GGGCCTATTT AGGAGATACC TCTGAGGCCA AGGAGTTTGC CAAGCAGTTC
    CTCGAGCGCC GTGCCAAACA GAAAGCCAGC CAGCGGCAGC AGCAGCAGCA GCAACAACAG
    CAACAGCAGC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGCAGGACTC TGTGTGGGGA
    ATGAACCACA GTGCACTCCA TTCAGTATTT CAGACCAATC AAAGCAACAA CCAACAATCC
    AATTTTGAGG CCGTACAAAG TGGCAAGAAG AAGAAAAAGC AGAAGATGGT CCGAGCAGAT
    CCCAGTTTGT TAGGATTTTC AGTCAATGCT TCATCAGAGC GACTCAATAT GGGTGAAATC
    GAGACTTTGG ATGACTACTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297287
    Clone ID Related Accession (Same CDS sequence) XM_022410023.1
    Accession Version XM_022410023.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3699bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCACAG GACTCTGTGT GGGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265731.1
    CDS215..3913
    Translation

    Target ORF information:

    RefSeq Version XM_022410023.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410023.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACCCA GTGGTGTGAG
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACTTT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TTAGGGCCTA TTTAGGAGAT
    ACCTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTCGAGC GCCGTGCCAA ACAGAAAGCC
    AGCCAGCGGC AGCAGCAGCA GCAGCAACAA CAGCAACAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCACAG GACTCTGTGT GGGGAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297288
    Clone ID Related Accession (Same CDS sequence) XM_022410024.1
    Accession Version XM_022410024.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3240bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACAGGG TCTGGACTGA GCGAGAAGAG AAATTCCAGC GGATCAAAAA CAAACAAGAT 
    GGCCCTCGTT CTGCAGGCTG GCGAGAACAC ATGGAACGAC GGCGAAGGTT TGAGTTTGAT
    TTTCGAGATC GGGATGATGA ACGGGGTTAT CGAAGGGTGC GCTCTGGCAG TGGAAGCATA
    GACGATGACC GGGATAGTTT GCCCGAGTGG TGCTTAGAGG ACGCTGAAGA AGAGATGGGC
    ACATTTGACT CCTCTGGAGC ATTCCTCTTT CTAAAGAAAG TACAGAAAGA GCCAATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GAGGAAGGTG GGGAGTGCTC TGACTCTGAG
    AGCAGCCATA ATGAAGAAGC CAAAGAACCT GATAAGACAA ATAGGAAAGA GGGAGAGAAG
    ACAGATAGAG TAGGAGTTGA AGCTAGTGAG GAAACATCCC AGACCTCATC ATCATCTGCA
    AGACCAGGTA CTCCTTCAGA CCATCAGCCT CAGGAAGCAC CGCAATTTGA GAGGAAGGAG
    GAATCCCAAA CTGAGCAAAC GGAAAAAGCT GAGGAAGAGG GTCAGACGGA AAATAGCCTG
    CCAACCAAAG TGCCCAGCAG AGGGGATGAA ATGGTTTCTG ATGTCCAGCA GCCCCTGTCA
    CAGATTCCTT CAGATACAGC CTCTCCTCTT CTCATACTTC CACCGCCTGT TCCCAATCCT
    AGTCCTGCCC TCCGGCCAGT TGAAACGCCG GTCGTAGGTG CTCCTGGTAT GGGCAGTGTT
    TCCACAGAGC CAGATGATGA AGAGGGTCTC AAGCATTTGG AGCAGCAAGC TGAGAAGATG
    GTGGCTTATC TCCAAGACAG TGCACTAGAT GATGAGAGAC TGGCATCAAA ACTGCAAGAG
    CACAGAGCTA AGGGAGTCTC AATTCCATTG ATGCACGAAG CAATGCAGAA GTGGTATTAC
    AAAGATCCTC AGGGAGAAAT TCAAGGTCCC TTCAATAATC AGGAGATGGC GGAATGGTTT
    CAGGCGGGTT ATTTTACTAT GTCTTTATTG GTGAAGAGAG CATGTGATGA AAGCTTCCAA
    CCTCTTGGTG ATATCATGAA AATGTGGGGA AGGGTTCCCT TCTCTCCCGG CCCAGCTCCC
    CCTCCTCATA TGGGAGAGTT GGACCAGGAA CGACTGACCA GGCAGCAGGA GCTCACTGCC
    TTGTACCAAA TGCAGCACCT TCAGTACCAG CAGTTCTTAA TACAACAGCA GTATGCACAG
    GTTTTGGCTC AACAACAGAA AGCAGCTTTG TCTTCACAGC AGCAGCAGCA GTTGGCACTT
    CTTCTCCAAC AGTTCCAGGC CCTGAAAATG AGAATATCTG ATCAGAACAT CATTCCTTCA
    GTAACTAGGT CTGTGTCGGT GCCAGATACT GGTTCTATCT GGGAGCTTCA ACCAACAGCC
    TCACAGCCTA CAGTTTGGGA AGGTGGTAGT GTATGGGATC TTCCCCTTGA CACCACAACT
    CCAGGACCAG CCCTTGAACA GCTTCAGCAG ATGGAGAAGG CCAAAGCTGC AAAGCTAGAA
    CAGGAGAGAA GAGAGGCAGA AATGAGGGCA AAACGGGAAG AGGAGGAACG AAAGAGGCAA
    GAAGAACTCC GGAGACAACA GGAGGAAATT CTTCGGAGAC AGCAGGAGGA GGAAAGGAAG
    AGGCGGGAGG AAGAAGAGCT CGCCCGAAGG AAACAGGAAG AAGCTCTGCG ACGCCAAAGG
    GAGCAAGAAA TTGCATTAAG GCGACAGCGA GAAGAGGAAG AAAGACAACA GCAAGAAGAA
    GCTCTTAGGA GACTGGAGGA GAGGAGGAGA GAAGAGGAAG AAAGGCGGAA GCAGGAGGAG
    TTGTTACGCA AGCAGGAAGA GGAAGCTGCA AAATGGGCCA GGGAAGAAGA AGAAGCCCAG
    CGTCGATTAG AGGAGAACCG GCTGCGAATG GAAGAGGAGG CAGCCCGACT CCGGCATGAA
    GAAGAGGAGC GGAAGAGGAA AGAGTTAGAG CTCCAGCGGC AGAAGGAGTT AATGCGCCAG
    AGGCAGCAGC AACAAGAGGC TCTCCGGAGG TTGCAGCAGC AACAGCAGCA GCAGCAGCTG
    GCTCAGATGA AGCTTCCCTC TTCTTCCACA TGGGGCCAGC AGTCCAATAC AACAGCATGT
    CAGTCCCAGG CAACGCTGTC ATTGGCTGAA ATCCAAAAAC TAGAAGAGGA ACGAGAACGG
    CAGCTTCGAG AGGAGCAAAG ACGCCAGCAG AGGGAACTGA TGAAAGCTCT GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGAA ACTCTCTGGA TGGGGGAATG TCAGTAAACC TGCAGGCACT
    GCGAAGTCTC TCTTGGAGAT ACAGCAGGAA GAAGCCAGGC AGATGCAGAA GCAGCAACAG
    CAGCAGCAGC AGCACCAGCA ACCAAACAGA GCCCGGAATA ATACGCATTC CAACCTGCAC
    ACCAGCATTG GGAATTCTGT TTGGGGCTCT ATAAATACTG GTCCCCCTAA CCAGTGGGCA
    TCTGACCTAG TCAGTAGTAT CTGGAGTAAT GCTGACACTA AAAACTCCAA CATGGGATTC
    TGGGATGATG CAGTGAAAGA GGTGGGACCT AGGAATTCTA CAAATAAAAA TAAAAACAAC
    GCCAGTCTCA GTAAATCTGT AGGTGTGTCT AACCGGCAGA ATAAGAAAGT AGAAGAAGAA
    GAAAAGTTGC TGAAGCTCTT TCAGGGAGTA AATAAAGCCC AAGATGGATT TACCCAGTGG
    TGTGAGCAGA TGCTTCATGC CCTTAATACG GCAAATAACT TGGATGTTCC CACTTTTGTT
    TCTTTCCTGA AAGAAGTAGA ATCTCCTTAT GAGGTCCATG ATTATATTAG GGCCTATTTA
    GGAGATACCT CTGAGGCCAA GGAGTTTGCC AAGCAGTTCC TCGAGCGCCG TGCCAAACAG
    AAAGCCAGCC AGCGGCAGCA GCAGCAGCAG CAACAACAGC AACAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGGACTCT GTGTGGGGAA TGAACCACAG TGCACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC CGTACAAAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTGTT AGGATTTTCA
    GTCAATGCTT CATCAGAGCG ACTCAATATG GGTGAAATCG AGACTTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265732.1
    CDS100..3339
    Translation

    Target ORF information:

    RefSeq Version XM_022410024.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X12, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410024.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACAGGG TCTGGACTGA GCGAGAAGAG AAATTCCAGC GGATCAAAAA CAAACAAGAT 
    GGCCCTCGTT CTGCAGGCTG GCGAGAACAC ATGGAACGAC GGCGAAGGTT TGAGTTTGAT
    TTTCGAGATC GGGATGATGA ACGGGGTTAT CGAAGGGTGC GCTCTGGCAG TGGAAGCATA
    GACGATGACC GGGATAGTTT GCCCGAGTGG TGCTTAGAGG ACGCTGAAGA AGAGATGGGC
    ACATTTGACT CCTCTGGAGC ATTCCTCTTT CTAAAGAAAG TACAGAAAGA GCCAATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GAGGAAGGTG GGGAGTGCTC TGACTCTGAG
    AGCAGCCATA ATGAAGAAGC CAAAGAACCT GATAAGACAA ATAGGAAAGA GGGAGAGAAG
    ACAGATAGAG TAGGAGTTGA AGCTAGTGAG GAAACATCCC AGACCTCATC ATCATCTGCA
    AGACCAGGTA CTCCTTCAGA CCATCAGCCT CAGGAAGCAC CGCAATTTGA GAGGAAGGAG
    GAATCCCAAA CTGAGCAAAC GGAAAAAGCT GAGGAAGAGG GTCAGACGGA AAATAGCCTG
    CCAACCAAAG TGCCCAGCAG AGGGGATGAA ATGGTTTCTG ATGTCCAGCA GCCCCTGTCA
    CAGATTCCTT CAGATACAGC CTCTCCTCTT CTCATACTTC CACCGCCTGT TCCCAATCCT
    AGTCCTGCCC TCCGGCCAGT TGAAACGCCG GTCGTAGGTG CTCCTGGTAT GGGCAGTGTT
    TCCACAGAGC CAGATGATGA AGAGGGTCTC AAGCATTTGG AGCAGCAAGC TGAGAAGATG
    GTGGCTTATC TCCAAGACAG TGCACTAGAT GATGAGAGAC TGGCATCAAA ACTGCAAGAG
    CACAGAGCTA AGGGAGTCTC AATTCCATTG ATGCACGAAG CAATGCAGAA GTGGTATTAC
    AAAGATCCTC AGGGAGAAAT TCAAGGTCCC TTCAATAATC AGGAGATGGC GGAATGGTTT
    CAGGCGGGTT ATTTTACTAT GTCTTTATTG GTGAAGAGAG CATGTGATGA AAGCTTCCAA
    CCTCTTGGTG ATATCATGAA AATGTGGGGA AGGGTTCCCT TCTCTCCCGG CCCAGCTCCC
    CCTCCTCATA TGGGAGAGTT GGACCAGGAA CGACTGACCA GGCAGCAGGA GCTCACTGCC
    TTGTACCAAA TGCAGCACCT TCAGTACCAG CAGTTCTTAA TACAACAGCA GTATGCACAG
    GTTTTGGCTC AACAACAGAA AGCAGCTTTG TCTTCACAGC AGCAGCAGCA GTTGGCACTT
    CTTCTCCAAC AGTTCCAGGC CCTGAAAATG AGAATATCTG ATCAGAACAT CATTCCTTCA
    GTAACTAGGT CTGTGTCGGT GCCAGATACT GGTTCTATCT GGGAGCTTCA ACCAACAGCC
    TCACAGCCTA CAGTTTGGGA AGGTGGTAGT GTATGGGATC TTCCCCTTGA CACCACAACT
    CCAGGACCAG CCCTTGAACA GCTTCAGCAG ATGGAGAAGG CCAAAGCTGC AAAGCTAGAA
    CAGGAGAGAA GAGAGGCAGA AATGAGGGCA AAACGGGAAG AGGAGGAACG AAAGAGGCAA
    GAAGAACTCC GGAGACAACA GGAGGAAATT CTTCGGAGAC AGCAGGAGGA GGAAAGGAAG
    AGGCGGGAGG AAGAAGAGCT CGCCCGAAGG AAACAGGAAG AAGCTCTGCG ACGCCAAAGG
    GAGCAAGAAA TTGCATTAAG GCGACAGCGA GAAGAGGAAG AAAGACAACA GCAAGAAGAA
    GCTCTTAGGA GACTGGAGGA GAGGAGGAGA GAAGAGGAAG AAAGGCGGAA GCAGGAGGAG
    TTGTTACGCA AGCAGGAAGA GGAAGCTGCA AAATGGGCCA GGGAAGAAGA AGAAGCCCAG
    CGTCGATTAG AGGAGAACCG GCTGCGAATG GAAGAGGAGG CAGCCCGACT CCGGCATGAA
    GAAGAGGAGC GGAAGAGGAA AGAGTTAGAG CTCCAGCGGC AGAAGGAGTT AATGCGCCAG
    AGGCAGCAGC AACAAGAGGC TCTCCGGAGG TTGCAGCAGC AACAGCAGCA GCAGCAGCTG
    GCTCAGATGA AGCTTCCCTC TTCTTCCACA TGGGGCCAGC AGTCCAATAC AACAGCATGT
    CAGTCCCAGG CAACGCTGTC ATTGGCTGAA ATCCAAAAAC TAGAAGAGGA ACGAGAACGG
    CAGCTTCGAG AGGAGCAAAG ACGCCAGCAG AGGGAACTGA TGAAAGCTCT GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGAA ACTCTCTGGA TGGGGGAATG TCAGTAAACC TGCAGGCACT
    GCGAAGTCTC TCTTGGAGAT ACAGCAGGAA GAAGCCAGGC AGATGCAGAA GCAGCAACAG
    CAGCAGCAGC AGCACCAGCA ACCAAACAGA GCCCGGAATA ATACGCATTC CAACCTGCAC
    ACCAGCATTG GGAATTCTGT TTGGGGCTCT ATAAATACTG GTCCCCCTAA CCAGTGGGCA
    TCTGACCTAG TCAGTAGTAT CTGGAGTAAT GCTGACACTA AAAACTCCAA CATGGGATTC
    TGGGATGATG CAGTGAAAGA GGTGGGACCT AGGAATTCTA CAAATAAAAA TAAAAACAAC
    GCCAGTCTCA GTAAATCTGT AGGTGTGTCT AACCGGCAGA ATAAGAAAGT AGAAGAAGAA
    GAAAAGTTGC TGAAGCTCTT TCAGGGAGTA AATAAAGCCC AAGATGGATT TACCCAGTGG
    TGTGAGCAGA TGCTTCATGC CCTTAATACG GCAAATAACT TGGATGTTCC CACTTTTGTT
    TCTTTCCTGA AAGAAGTAGA ATCTCCTTAT GAGGTCCATG ATTATATTAG GGCCTATTTA
    GGAGATACCT CTGAGGCCAA GGAGTTTGCC AAGCAGTTCC TCGAGCGCCG TGCCAAACAG
    AAAGCCAGCC AGCGGCAGCA GCAGCAGCAG CAACAACAGC AACAGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGCAGCAGCA GCAGGACTCT GTGTGGGGAA TGAACCACAG TGCACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC CGTACAAAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTGTT AGGATTTTCA
    GTCAATGCTT CATCAGAGCG ACTCAATATG GGTGAAATCG AGACTTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297289
    Clone ID Related Accession (Same CDS sequence) XM_022410025.1
    Accession Version XM_022410025.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3351bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X12
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTTCCC ACTTTTGTTT CTTTCCTGAA AGAAGTAGAA TCTCCTTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265733.1
    CDS215..3565
    Translation

    Target ORF information:

    RefSeq Version XM_022410025.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X13, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410025.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCAGCGG AAACACAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTATCCAGT 
    GGTGGGAGTA TCACGTCCCC TCCTCTTTCT CCAGCTTTGC CGAAGTATAA GTTAGCAGAC
    TATCGTTATG GTCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGATAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATT CTACAGGAGG AGCCCCTGCC TCCGTTGGCT
    CTTGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCCGCT
    GTCCTGAGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT
    GATGAAGTAG AGGGTGTGTT TGGTCGAGGA GGTGGCAGGG AAATGCATAG GTCACAGAGC
    TGGGAGGAAA GAGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCGGTG GGAAGAAAGC ATGAGTTTAT ACGATCAGAA
    AGTGAAAACT GGCGTATTTT TAGAGAAGAA CAAAACGGAG AAGATGAAGA TGGAGGTTGG
    AGACTAGCTG GACCGAGACG GGATGGAGAG AGGTGGCGTC CCCATAGCCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGAGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA
    GATCGGGATG ATGAACGGGG TTATCGAAGG GTGCGCTCTG GCAGTGGAAG CATAGACGAT
    GACCGGGATA GTTTGCCCGA GTGGTGCTTA GAGGACGCTG AAGAAGAGAT GGGCACATTT
    GACTCCTCTG GAGCATTCCT CTTTCTAAAG AAAGTACAGA AAGAGCCAAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGAGGAA GGTGGGGAGT GCTCTGACTC TGAGAGCAGC
    CATAATGAAG AAGCCAAAGA ACCTGATAAG ACAAATAGGA AAGAGGGAGA GAAGACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACA TCCCAGACCT CATCATCATC TGCAAGACCA
    GGTACTCCTT CAGACCATCA GCCTCAGGAA GCACCGCAAT TTGAGAGGAA GGAGGAATCC
    CAAACTGAGC AAACGGAAAA AGCTGAGGAA GAGGGTCAGA CGGAAAATAG CCTGCCAACC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT TCTGATGTCC AGCAGCCCCT GTCACAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCGC CTGTTCCCAA TCCTAGTCCT
    GCCCTCCGGC CAGTTGAAAC GCCGGTCGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAGCCAGATG ATGAAGAGGG TCTCAAGCAT TTGGAGCAGC AAGCTGAGAA GATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAG AGACTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAGGGAG TCTCAATTCC ATTGATGCAC GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCGGAATG GTTTCAGGCG
    GGTTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCATGTG ATGAAAGCTT CCAACCTCTT
    GGTGATATCA TGAAAATGTG GGGAAGGGTT CCCTTCTCTC CCGGCCCAGC TCCCCCTCCT
    CATATGGGAG AGTTGGACCA GGAACGACTG ACCAGGCAGC AGGAGCTCAC TGCCTTGTAC
    CAAATGCAGC ACCTTCAGTA CCAGCAGTTC TTAATACAAC AGCAGTATGC ACAGGTTTTG
    GCTCAACAAC AGAAAGCAGC TTTGTCTTCA CAGCAGCAGC AGCAGTTGGC ACTTCTTCTC
    CAACAGTTCC AGGCCCTGAA AATGAGAATA TCTGATCAGA ACATCATTCC TTCAGTAACT
    AGGTCTGTGT CGGTGCCAGA TACTGGTTCT ATCTGGGAGC TTCAACCAAC AGCCTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCCC TTGACACCAC AACTCCAGGA
    CCAGCCCTTG AACAGCTTCA GCAGATGGAG AAGGCCAAAG CTGCAAAGCT AGAACAGGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAGG AACGAAAGAG GCAAGAAGAA
    CTCCGGAGAC AACAGGAGGA AATTCTTCGG AGACAGCAGG AGGAGGAAAG GAAGAGGCGG
    GAGGAAGAAG AGCTCGCCCG AAGGAAACAG GAAGAAGCTC TGCGACGCCA AAGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AACAGCAAGA AGAAGCTCTT
    AGGAGACTGG AGGAGAGGAG GAGAGAAGAG GAAGAAAGGC GGAAGCAGGA GGAGTTGTTA
    CGCAAGCAGG AAGAGGAAGC TGCAAAATGG GCCAGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG AATGGAAGAG GAGGCAGCCC GACTCCGGCA TGAAGAAGAG
    GAGCGGAAGA GGAAAGAGTT AGAGCTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAACAAG AGGCTCTCCG GAGGTTGCAG CAGCAACAGC AGCAGCAGCA GCTGGCTCAG
    ATGAAGCTTC CCTCTTCTTC CACATGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCAACGC TGTCATTGGC TGAAATCCAA AAACTAGAAG AGGAACGAGA ACGGCAGCTT
    CGAGAGGAGC AAAGACGCCA GCAGAGGGAA CTGATGAAAG CTCTGCAGCA GCAGCAGCAG
    CAGCAGCAGC AGAAACTCTC TGGATGGGGG AATGTCAGTA AACCTGCAGG CACTGCGAAG
    TCTCTCTTGG AGATACAGCA GGAAGAAGCC AGGCAGATGC AGAAGCAGCA ACAGCAGCAG
    CAGCAGCACC AGCAACCAAA CAGAGCCCGG AATAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCCC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATCTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCTACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTTCCC ACTTTTGTTT CTTTCCTGAA AGAAGTAGAA TCTCCTTATG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OCe297290
    Clone ID Related Accession (Same CDS sequence) XM_022410026.1
    Accession Version XM_022410026.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-09-04
    Organism Canis lupus familiaris(dog)
    Product GRB10-interacting GYF protein 2 isoform X13
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_006607.3) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Canis lupus familiaris Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACAGGG TCTGGACTGA GCGAGAAGAG AAATTCCAGC GGATCAAAAA CAAACAAGGC 
    TGGCGAGAAC ACATGGAACG ACGGCGAAGG TTTGAGTTTG ATTTTCGAGA TCGGGATGAT
    GAACGGGGTT ATCGAAGGGT GCGCTCTGGC AGTGGAAGCA TAGACGATGA CCGGGATAGT
    TTGCCCGAGT GGTGCTTAGA GGACGCTGAA GAAGAGATGG GCACATTTGA CTCCTCTGGA
    GCATTCCTCT TTCTAAAGAA AGTACAGAAA GAGCCAATTC CAGAAGAGCA GGAGATGGAC
    TTCCGGCCTG TGGAGGAAGG TGGGGAGTGC TCTGACTCTG AGAGCAGCCA TAATGAAGAA
    GCCAAAGAAC CTGATAAGAC AAATAGGAAA GAGGGAGAGA AGACAGATAG AGTAGGAGTT
    GAAGCTAGTG AGGAAACATC CCAGACCTCA TCATCATCTG CAAGACCAGG TACTCCTTCA
    GACCATCAGC CTCAGGAAGC ACCGCAATTT GAGAGGAAGG AGGAATCCCA AACTGAGCAA
    ACGGAAAAAG CTGAGGAAGA GGGTCAGACG GAAAATAGCC TGCCAACCAA AGTGCCCAGC
    AGAGGGGATG AAATGGTTTC TGATGTCCAG CAGCCCCTGT CACAGATTCC TTCAGATACA
    GCCTCTCCTC TTCTCATACT TCCACCGCCT GTTCCCAATC CTAGTCCTGC CCTCCGGCCA
    GTTGAAACGC CGGTCGTAGG TGCTCCTGGT ATGGGCAGTG TTTCCACAGA GCCAGATGAT
    GAAGAGGGTC TCAAGCATTT GGAGCAGCAA GCTGAGAAGA TGGTGGCTTA TCTCCAAGAC
    AGTGCACTAG ATGATGAGAG ACTGGCATCA AAACTGCAAG AGCACAGAGC TAAGGGAGTC
    TCAATTCCAT TGATGCACGA AGCAATGCAG AAGTGGTATT ACAAAGATCC TCAGGGAGAA
    ATTCAAGGTC CCTTCAATAA TCAGGAGATG GCGGAATGGT TTCAGGCGGG TTATTTTACT
    ATGTCTTTAT TGGTGAAGAG AGCATGTGAT GAAAGCTTCC AACCTCTTGG TGATATCATG
    AAAATGTGGG GAAGGGTTCC CTTCTCTCCC GGCCCAGCTC CCCCTCCTCA TATGGGAGAG
    TTGGACCAGG AACGACTGAC CAGGCAGCAG GAGCTCACTG CCTTGTACCA AATGCAGCAC
    CTTCAGTACC AGCAGTTCTT AATACAACAG CAGTATGCAC AGGTTTTGGC TCAACAACAG
    AAAGCAGCTT TGTCTTCACA GCAGCAGCAG CAGTTGGCAC TTCTTCTCCA ACAGTTCCAG
    GCCCTGAAAA TGAGAATATC TGATCAGAAC ATCATTCCTT CAGTAACTAG GTCTGTGTCG
    GTGCCAGATA CTGGTTCTAT CTGGGAGCTT CAACCAACAG CCTCACAGCC TACAGTTTGG
    GAAGGTGGTA GTGTATGGGA TCTTCCCCTT GACACCACAA CTCCAGGACC AGCCCTTGAA
    CAGCTTCAGC AGATGGAGAA GGCCAAAGCT GCAAAGCTAG AACAGGAGAG AAGAGAGGCA
    GAAATGAGGG CAAAACGGGA AGAGGAGGAA CGAAAGAGGC AAGAAGAACT CCGGAGACAA
    CAGGAGGAAA TTCTTCGGAG ACAGCAGGAG GAGGAAAGGA AGAGGCGGGA GGAAGAAGAG
    CTCGCCCGAA GGAAACAGGA AGAAGCTCTG CGACGCCAAA GGGAGCAAGA AATTGCATTA
    AGGCGACAGC GAGAAGAGGA AGAAAGACAA CAGCAAGAAG AAGCTCTTAG GAGACTGGAG
    GAGAGGAGGA GAGAAGAGGA AGAAAGGCGG AAGCAGGAGG AGTTGTTACG CAAGCAGGAA
    GAGGAAGCTG CAAAATGGGC CAGGGAAGAA GAAGAAGCCC AGCGTCGATT AGAGGAGAAC
    CGGCTGCGAA TGGAAGAGGA GGCAGCCCGA CTCCGGCATG AAGAAGAGGA GCGGAAGAGG
    AAAGAGTTAG AGCTCCAGCG GCAGAAGGAG TTAATGCGCC AGAGGCAGCA GCAACAAGAG
    GCTCTCCGGA GGTTGCAGCA GCAACAGCAG CAGCAGCAGC TGGCTCAGAT GAAGCTTCCC
    TCTTCTTCCA CATGGGGCCA GCAGTCCAAT ACAACAGCAT GTCAGTCCCA GGCAACGCTG
    TCATTGGCTG AAATCCAAAA ACTAGAAGAG GAACGAGAAC GGCAGCTTCG AGAGGAGCAA
    AGACGCCAGC AGAGGGAACT GATGAAAGCT CTGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    AAACTCTCTG GATGGGGGAA TGTCAGTAAA CCTGCAGGCA CTGCGAAGTC TCTCTTGGAG
    ATACAGCAGG AAGAAGCCAG GCAGATGCAG AAGCAGCAAC AGCAGCAGCA GCAGCACCAG
    CAACCAAACA GAGCCCGGAA TAATACGCAT TCCAACCTGC ACACCAGCAT TGGGAATTCT
    GTTTGGGGCT CTATAAATAC TGGTCCCCCT AACCAGTGGG CATCTGACCT AGTCAGTAGT
    ATCTGGAGTA ATGCTGACAC TAAAAACTCC AACATGGGAT TCTGGGATGA TGCAGTGAAA
    GAGGTGGGAC CTAGGAATTC TACAAATAAA AATAAAAACA ACGCCAGTCT CAGTAAATCT
    GTAGGTGTGT CTAACCGGCA GAATAAGAAA GTAGAAGAAG AAGAAAAGTT GCTGAAGCTC
    TTTCAGGGAG TAAATAAAGC CCAAGATGGA TTTACCCAGT GGTGTGAGCA GATGCTTCAT
    GCCCTTAATA CGGCAAATAA CTTGGATGTT CCCACTTTTG TTTCTTTCCT GAAAGAAGTA
    GAATCTCCTT ATGAGGTCCA TGATTATATT AGGGCCTATT TAGGAGATAC CTCTGAGGCC
    AAGGAGTTTG CCAAGCAGTT CCTCGAGCGC CGTGCCAAAC AGAAAGCCAG CCAGCGGCAG
    CAGCAGCAGC AGCAACAACA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGGACT CTGTGTGGGG AATGAACCAC AGTGCACTCC ATTCAGTATT TCAGACCAAT
    CAAAGCAACA ACCAACAATC CAATTTTGAG GCCGTACAAA GTGGCAAGAA GAAGAAAAAG
    CAGAAGATGG TCCGAGCAGA TCCCAGTTTG TTAGGATTTT CAGTCAATGC TTCATCAGAG
    CGACTCAATA TGGGTGAAAT CGAGACTTTG GATGACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_022265734.1
    CDS86..3307
    Translation

    Target ORF information:

    RefSeq Version XM_022410026.1
    Organism Canis lupus familiaris(dog)
    Definition Canis lupus familiaris GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X14, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_022410026.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGACAGGG TCTGGACTGA GCGAGAAGAG AAATTCCAGC GGATCAAAAA CAAACAAGGC 
    TGGCGAGAAC ACATGGAACG ACGGCGAAGG TTTGAGTTTG ATTTTCGAGA TCGGGATGAT
    GAACGGGGTT ATCGAAGGGT GCGCTCTGGC AGTGGAAGCA TAGACGATGA CCGGGATAGT
    TTGCCCGAGT GGTGCTTAGA GGACGCTGAA GAAGAGATGG GCACATTTGA CTCCTCTGGA
    GCATTCCTCT TTCTAAAGAA AGTACAGAAA GAGCCAATTC CAGAAGAGCA GGAGATGGAC
    TTCCGGCCTG TGGAGGAAGG TGGGGAGTGC TCTGACTCTG AGAGCAGCCA TAATGAAGAA
    GCCAAAGAAC CTGATAAGAC AAATAGGAAA GAGGGAGAGA AGACAGATAG AGTAGGAGTT
    GAAGCTAGTG AGGAAACATC CCAGACCTCA TCATCATCTG CAAGACCAGG TACTCCTTCA
    GACCATCAGC CTCAGGAAGC ACCGCAATTT GAGAGGAAGG AGGAATCCCA AACTGAGCAA
    ACGGAAAAAG CTGAGGAAGA GGGTCAGACG GAAAATAGCC TGCCAACCAA AGTGCCCAGC
    AGAGGGGATG AAATGGTTTC TGATGTCCAG CAGCCCCTGT CACAGATTCC TTCAGATACA
    GCCTCTCCTC TTCTCATACT TCCACCGCCT GTTCCCAATC CTAGTCCTGC CCTCCGGCCA
    GTTGAAACGC CGGTCGTAGG TGCTCCTGGT ATGGGCAGTG TTTCCACAGA GCCAGATGAT
    GAAGAGGGTC TCAAGCATTT GGAGCAGCAA GCTGAGAAGA TGGTGGCTTA TCTCCAAGAC
    AGTGCACTAG ATGATGAGAG ACTGGCATCA AAACTGCAAG AGCACAGAGC TAAGGGAGTC
    TCAATTCCAT TGATGCACGA AGCAATGCAG AAGTGGTATT ACAAAGATCC TCAGGGAGAA
    ATTCAAGGTC CCTTCAATAA TCAGGAGATG GCGGAATGGT TTCAGGCGGG TTATTTTACT
    ATGTCTTTAT TGGTGAAGAG AGCATGTGAT GAAAGCTTCC AACCTCTTGG TGATATCATG
    AAAATGTGGG GAAGGGTTCC CTTCTCTCCC GGCCCAGCTC CCCCTCCTCA TATGGGAGAG
    TTGGACCAGG AACGACTGAC CAGGCAGCAG GAGCTCACTG CCTTGTACCA AATGCAGCAC
    CTTCAGTACC AGCAGTTCTT AATACAACAG CAGTATGCAC AGGTTTTGGC TCAACAACAG
    AAAGCAGCTT TGTCTTCACA GCAGCAGCAG CAGTTGGCAC TTCTTCTCCA ACAGTTCCAG
    GCCCTGAAAA TGAGAATATC TGATCAGAAC ATCATTCCTT CAGTAACTAG GTCTGTGTCG
    GTGCCAGATA CTGGTTCTAT CTGGGAGCTT CAACCAACAG CCTCACAGCC TACAGTTTGG
    GAAGGTGGTA GTGTATGGGA TCTTCCCCTT GACACCACAA CTCCAGGACC AGCCCTTGAA
    CAGCTTCAGC AGATGGAGAA GGCCAAAGCT GCAAAGCTAG AACAGGAGAG AAGAGAGGCA
    GAAATGAGGG CAAAACGGGA AGAGGAGGAA CGAAAGAGGC AAGAAGAACT CCGGAGACAA
    CAGGAGGAAA TTCTTCGGAG ACAGCAGGAG GAGGAAAGGA AGAGGCGGGA GGAAGAAGAG
    CTCGCCCGAA GGAAACAGGA AGAAGCTCTG CGACGCCAAA GGGAGCAAGA AATTGCATTA
    AGGCGACAGC GAGAAGAGGA AGAAAGACAA CAGCAAGAAG AAGCTCTTAG GAGACTGGAG
    GAGAGGAGGA GAGAAGAGGA AGAAAGGCGG AAGCAGGAGG AGTTGTTACG CAAGCAGGAA
    GAGGAAGCTG CAAAATGGGC CAGGGAAGAA GAAGAAGCCC AGCGTCGATT AGAGGAGAAC
    CGGCTGCGAA TGGAAGAGGA GGCAGCCCGA CTCCGGCATG AAGAAGAGGA GCGGAAGAGG
    AAAGAGTTAG AGCTCCAGCG GCAGAAGGAG TTAATGCGCC AGAGGCAGCA GCAACAAGAG
    GCTCTCCGGA GGTTGCAGCA GCAACAGCAG CAGCAGCAGC TGGCTCAGAT GAAGCTTCCC
    TCTTCTTCCA CATGGGGCCA GCAGTCCAAT ACAACAGCAT GTCAGTCCCA GGCAACGCTG
    TCATTGGCTG AAATCCAAAA ACTAGAAGAG GAACGAGAAC GGCAGCTTCG AGAGGAGCAA
    AGACGCCAGC AGAGGGAACT GATGAAAGCT CTGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    AAACTCTCTG GATGGGGGAA TGTCAGTAAA CCTGCAGGCA CTGCGAAGTC TCTCTTGGAG
    ATACAGCAGG AAGAAGCCAG GCAGATGCAG AAGCAGCAAC AGCAGCAGCA GCAGCACCAG
    CAACCAAACA GAGCCCGGAA TAATACGCAT TCCAACCTGC ACACCAGCAT TGGGAATTCT
    GTTTGGGGCT CTATAAATAC TGGTCCCCCT AACCAGTGGG CATCTGACCT AGTCAGTAGT
    ATCTGGAGTA ATGCTGACAC TAAAAACTCC AACATGGGAT TCTGGGATGA TGCAGTGAAA
    GAGGTGGGAC CTAGGAATTC TACAAATAAA AATAAAAACA ACGCCAGTCT CAGTAAATCT
    GTAGGTGTGT CTAACCGGCA GAATAAGAAA GTAGAAGAAG AAGAAAAGTT GCTGAAGCTC
    TTTCAGGGAG TAAATAAAGC CCAAGATGGA TTTACCCAGT GGTGTGAGCA GATGCTTCAT
    GCCCTTAATA CGGCAAATAA CTTGGATGTT CCCACTTTTG TTTCTTTCCT GAAAGAAGTA
    GAATCTCCTT ATGAGGTCCA TGATTATATT AGGGCCTATT TAGGAGATAC CTCTGAGGCC
    AAGGAGTTTG CCAAGCAGTT CCTCGAGCGC CGTGCCAAAC AGAAAGCCAG CCAGCGGCAG
    CAGCAGCAGC AGCAACAACA GCAACAGCAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG
    CAGCAGGACT CTGTGTGGGG AATGAACCAC AGTGCACTCC ATTCAGTATT TCAGACCAAT
    CAAAGCAACA ACCAACAATC CAATTTTGAG GCCGTACAAA GTGGCAAGAA GAAGAAAAAG
    CAGAAGATGG TCCGAGCAGA TCCCAGTTTG TTAGGATTTT CAGTCAATGC TTCATCAGAG
    CGACTCAATA TGGGTGAAAT CGAGACTTTG GATGACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.