GIGYF2 cDNA ORF clone, Homo sapiens(human)

The following GIGYF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIGYF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OHu17213 NM_001103147.2
Latest version!
Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17353 NM_001103146.3
Latest version!
Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $643.30
$919.00
OHu17216 NM_001103148.2
Latest version!
Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $909.30
$1299.00
OHu17353 NM_015575.4
Latest version!
Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $916.30
$1309.00
View Old Accession Versions >>
OHu17353 NM_015575.3 Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $916.30
$1309.00
OHu17213 NM_001103147.1 Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 1, mRNA. pcDNA3.1-C-(k)DYK or customized vector
in pcDNA3.1-C-(k)DYK
$459.50-$643.30
$919.00
OHu17353 NM_001103146.1 Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $916.30
$1309.00
OHu17353 NM_001103146.2 Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $916.30
$1309.00
OHu17216 NM_001103148.1 Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD $909.30
$1299.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OHu17213
    Clone ID Related Accession (Same CDS sequence) NM_001103147.1 , NM_001103147.2
    Accession Version NM_001103147.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-20
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG527686.1, BC146775.1, AC016692.9 and BE220828.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146775.1, AB014542.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGAAAGAAG
    AATGGATATT ATTGCATGTA CTCACCAGTT CTTTTGCTGG GTCAGCCTTT ATGCCAAGGG
    AGACCAAATT TTGAGGAAGG TGGACCAACA TCAGTAGGGA GAAAGCATGA ATTTATACGC
    TCAGAAAGTG AAAATTGGCG CATCTTTAGA GAGGAACAAA ATGGAGAAGA TGAAGATGGA
    GGTTGGCGAC TAGCTGGATC AAGGAGGGAT GGAGAGAGGT GGCGACCTCA CAGTCCTGAT
    GGCCCTCGTT CTGCAGGCTG GCGGGAACAC ATGGAACGAC GTCGGAGGTT TGAGTTTGAT
    TTTCGAGATA GAGATGATGA ACGGGGTTAC CGAAGGGTTC GCTCTGGCAG TGGGAGCATA
    GATGATGACA GGGATAGCTT GCCCGAATGG TGCTTAGAGG ATGCTGAAGA AGAAATGGGT
    ACATTTGACT CATCTGGAGC ATTCCTTTCT CTAAAAAAAG TACAGAAAGA GCCTATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GACGAAGGGG AGGAGTGCTC TGACTCTGAG
    GGTAGCCATA ATGAAGAGGC CAAAGAACCC GATAAGACAA ATAAGAAAGA AGGAGAGAAA
    ACAGATAGAG TAGGAGTTGC TAGTGAGGAA ACTCCCCAGA CCTCATCATC ATCTGCTAGA
    CCAGGTACTC CTTCAGACCA TCAGTCTCAG GAAGCATCAC AGTTTGAGAG GAAAGATGAA
    CCAAAAACTG AGCAAACGGA AAAAGCTGAA GAGGAGACTC GGATGGAAAA TAGTCTACCA
    GCCAAAGTGC CCAGCAGAGG GGATGAAATG GTTGCTGATG TCCAGCAGCC CCTGTCGCAG
    ATTCCTTCAG ATACAGCCTC TCCTCTTCTC ATACTTCCAC CTCCTGTTCC CAATCCTAGT
    CCTACTCTCC GGCCAGTTGA AACACCAGTT GTAGGTGCTC CTGGTATGGG CAGTGTTTCC
    ACAGAACCTG ATGATGAAGA AGGTCTCAAA CATTTGGAGC AGCAAGCTGA GAAAATGGTG
    GCTTATCTCC AAGACAGTGC ACTAGATGAT GAAAGATTGG CATCAAAACT GCAAGAGCAC
    AGAGCTAAAG GAGTGTCGAT TCCATTGATG CATGAAGCAA TGCAGAAGTG GTATTACAAA
    GATCCTCAGG GAGAAATTCA AGGTCCCTTC AATAATCAGG AGATGGCAGA ATGGTTTCAG
    GCGGGCTATT TTACTATGTC TTTATTGGTG AAGAGAGCGT GTGATGAAAG CTTCCAACCT
    CTTGGCGATA TCATGAAAAT GTGGGGAAGG GTTCCCTTTT CTCCAGGTCC AGCTCCCCCT
    CCTCATATGG GAGAGCTGGA CCAGGAACGA CTGACCAGGC AGCAAGAACT CACAGCCTTA
    TACCAGATGC AGCACCTGCA GTACCAGCAG TTTTTAATAC AACAACAATA TGCACAGGTT
    TTGGCCCAAC AGCAGAAAGC AGCACTGTCT TCCCAGCAGC AGCAGCAGTT GGCACTTCTT
    CTTCAACAGT TTCAGACCTT GAAGATGAGA ATATCTGATC AGAACATCAT TCCCTCAGTA
    ACTAGGTCTG TGTCCGTGCC AGATACTGGC TCTATCTGGG AGCTTCAGCC AACAGCTTCA
    CAGCCTACAG TTTGGGAAGG TGGTAGTGTA TGGGATCTTC CTCTGGACAC CACGACACCA
    GGCCCTGCCC TGGAACAGCT TCAGCAGCTA GAGAAGGCCA AAGCTGCAAA GCTAGAGCAA
    GAGAGAAGAG AGGCAGAAAT GAGGGCAAAA CGGGAAGAGG AAGAGCGAAA GAGGCAGGAA
    GAACTCCGAA GACAACAGGA GGAAATTCTT CGGCGACAGC AGGAAGAAGA AAGGAAAAGG
    CGAGAGGAAG AAGAACTTGC CCGAAGGAAA CAGGAAGAGG CTCTGCGTCG CCAGCGGGAG
    CAAGAAATTG CATTAAGGCG ACAGCGAGAA GAGGAAGAAA GACAGCAGCA AGAAGAAGCT
    CTTAGAAGAC TGGAAGAGAG GAGAAGAGAA GAGGAAGAAA GGCGGAAGCA GGAAGAATTG
    TTACGCAAAC AGGAAGAGGA GGCTGCAAAA TGGGCCCGGG AAGAAGAAGA AGCCCAGCGT
    CGATTAGAGG AGAACCGGCT GCGGATGGAA GAGGAGGCAG CCAGACTCCG GCATGAGGAA
    GAAGAACGGA AGAGAAAGGA GCTGGAGGTC CAGCGGCAGA AGGAGTTAAT GCGCCAGAGG
    CAGCAGCAGC AAGAGGCTCT CCGGAGGTTG CAGCAGCAGC AGCAGCAACA ACAGCTGGCG
    CAGATGAAGC TTCCTTCTTC TTCAACGTGG GGCCAGCAGT CCAATACAAC AGCATGTCAG
    TCCCAGGCCA CGCTGTCGTT GGCTGAAATC CAAAAACTAG AGGAAGAACG AGAACGGCAG
    CTTCGAGAAG AGCAAAGGCG CCAGCAGAGG GAGTTGATGA AAGCTCTTCA GCAGCAGCAG
    CAACAGCAAC AGCAGAAACT CTCAGGTTGG GGGAATGTCA GCAAACCTTC AGGTACCACG
    AAATCTCTTC TGGAGATCCA GCAGGAAGAG GCCAGGCAAA TGCAAAAGCA GCAGCAGCAG
    CAGCAGCAAC ACCAGCAACC AAACAGAGCT CGTAACAATA CGCATTCCAA CCTGCACACC
    AGCATTGGGA ATTCTGTTTG GGGCTCTATA AATACTGGTC CTCCTAACCA GTGGGCATCT
    GACCTAGTCA GTAGTATTTG GAGTAATGCT GACACTAAAA ACTCCAACAT GGGATTCTGG
    GATGATGCAG TGAAAGAGGT GGGACCTAGG AATTCAACAA ATAAAAATAA AAACAACGCC
    AGTCTCAGTA AATCTGTAGG TGTGTCTAAC CGGCAGAATA AGAAAGTAGA AGAAGAAGAA
    AAGTTGCTGA AGCTCTTTCA GGGAGTAAAT AAAGCCCAAG ATGGATTTAC GCAGTGGTGT
    GAACAGATGC TTCATGCCCT TAATACGGCA AATAACTTGG ATGTTCCCAC ATTTGTTTCT
    TTCCTGAAAG AAGTAGAATC TCCTTATGAG GTCCATGATT ATATCAGGGC CTATTTAGGA
    GATACTTCTG AGGCCAAGGA GTTTGCCAAG CAGTTCCTTG AGCGCCGTGC CAAACAGAAA
    GCCAACCAGC AGCGTCAGCA GCAGCAGCTG CCACAGCAGC AGCAGCAGCA GCCGCCACAG
    CAGCCGCCAC AGCAGCCACA ACAGCAGGAC TCTGTGTGGG GGATGAACCA CAGTACACTC
    CATTCAGTAT TTCAGACCAA TCAAAGCAAC AACCAACAAT CCAATTTTGA GGCTGTGCAG
    AGTGGCAAGA AGAAGAAAAA GCAGAAGATG GTCCGAGCAG ATCCCAGTTT ATTAGGATTT
    TCAGTCAATG CATCATCGGA GCGACTCAAC ATGGGTGAAA TCGAGACGTT GGATGACTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096617.1
    CDS268..4230
    Translation

    Target ORF information:

    RefSeq Version NM_001103147.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103147.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGAAAGAAG
    AATGGATATT ATTGCATGTA CTCACCAGTT CTTTTGCTGG GTCAGCCTTT ATGCCAAGGG
    AGACCAAATT TTGAGGAAGG TGGACCAACA TCAGTAGGGA GAAAGCATGA ATTTATACGC
    TCAGAAAGTG AAAATTGGCG CATCTTTAGA GAGGAACAAA ATGGAGAAGA TGAAGATGGA
    GGTTGGCGAC TAGCTGGATC AAGGAGGGAT GGAGAGAGGT GGCGACCTCA CAGTCCTGAT
    GGCCCTCGTT CTGCAGGCTG GCGGGAACAC ATGGAACGAC GTCGGAGGTT TGAGTTTGAT
    TTTCGAGATA GAGATGATGA ACGGGGTTAC CGAAGGGTTC GCTCTGGCAG TGGGAGCATA
    GATGATGACA GGGATAGCTT GCCCGAATGG TGCTTAGAGG ATGCTGAAGA AGAAATGGGT
    ACATTTGACT CATCTGGAGC ATTCCTTTCT CTAAAAAAAG TACAGAAAGA GCCTATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GACGAAGGGG AGGAGTGCTC TGACTCTGAG
    GGTAGCCATA ATGAAGAGGC CAAAGAACCC GATAAGACAA ATAAGAAAGA AGGAGAGAAA
    ACAGATAGAG TAGGAGTTGC TAGTGAGGAA ACTCCCCAGA CCTCATCATC ATCTGCTAGA
    CCAGGTACTC CTTCAGACCA TCAGTCTCAG GAAGCATCAC AGTTTGAGAG GAAAGATGAA
    CCAAAAACTG AGCAAACGGA AAAAGCTGAA GAGGAGACTC GGATGGAAAA TAGTCTACCA
    GCCAAAGTGC CCAGCAGAGG GGATGAAATG GTTGCTGATG TCCAGCAGCC CCTGTCGCAG
    ATTCCTTCAG ATACAGCCTC TCCTCTTCTC ATACTTCCAC CTCCTGTTCC CAATCCTAGT
    CCTACTCTCC GGCCAGTTGA AACACCAGTT GTAGGTGCTC CTGGTATGGG CAGTGTTTCC
    ACAGAACCTG ATGATGAAGA AGGTCTCAAA CATTTGGAGC AGCAAGCTGA GAAAATGGTG
    GCTTATCTCC AAGACAGTGC ACTAGATGAT GAAAGATTGG CATCAAAACT GCAAGAGCAC
    AGAGCTAAAG GAGTGTCGAT TCCATTGATG CATGAAGCAA TGCAGAAGTG GTATTACAAA
    GATCCTCAGG GAGAAATTCA AGGTCCCTTC AATAATCAGG AGATGGCAGA ATGGTTTCAG
    GCGGGCTATT TTACTATGTC TTTATTGGTG AAGAGAGCGT GTGATGAAAG CTTCCAACCT
    CTTGGCGATA TCATGAAAAT GTGGGGAAGG GTTCCCTTTT CTCCAGGTCC AGCTCCCCCT
    CCTCATATGG GAGAGCTGGA CCAGGAACGA CTGACCAGGC AGCAAGAACT CACAGCCTTA
    TACCAGATGC AGCACCTGCA GTACCAGCAG TTTTTAATAC AACAACAATA TGCACAGGTT
    TTGGCCCAAC AGCAGAAAGC AGCACTGTCT TCCCAGCAGC AGCAGCAGTT GGCACTTCTT
    CTTCAACAGT TTCAGACCTT GAAGATGAGA ATATCTGATC AGAACATCAT TCCCTCAGTA
    ACTAGGTCTG TGTCCGTGCC AGATACTGGC TCTATCTGGG AGCTTCAGCC AACAGCTTCA
    CAGCCTACAG TTTGGGAAGG TGGTAGTGTA TGGGATCTTC CTCTGGACAC CACGACACCA
    GGCCCTGCCC TGGAACAGCT TCAGCAGCTA GAGAAGGCCA AAGCTGCAAA GCTAGAGCAA
    GAGAGAAGAG AGGCAGAAAT GAGGGCAAAA CGGGAAGAGG AAGAGCGAAA GAGGCAGGAA
    GAACTCCGAA GACAACAGGA GGAAATTCTT CGGCGACAGC AGGAAGAAGA AAGGAAAAGG
    CGAGAGGAAG AAGAACTTGC CCGAAGGAAA CAGGAAGAGG CTCTGCGTCG CCAGCGGGAG
    CAAGAAATTG CATTAAGGCG ACAGCGAGAA GAGGAAGAAA GACAGCAGCA AGAAGAAGCT
    CTTAGAAGAC TGGAAGAGAG GAGAAGAGAA GAGGAAGAAA GGCGGAAGCA GGAAGAATTG
    TTACGCAAAC AGGAAGAGGA GGCTGCAAAA TGGGCCCGGG AAGAAGAAGA AGCCCAGCGT
    CGATTAGAGG AGAACCGGCT GCGGATGGAA GAGGAGGCAG CCAGACTCCG GCATGAGGAA
    GAAGAACGGA AGAGAAAGGA GCTGGAGGTC CAGCGGCAGA AGGAGTTAAT GCGCCAGAGG
    CAGCAGCAGC AAGAGGCTCT CCGGAGGTTG CAGCAGCAGC AGCAGCAACA ACAGCTGGCG
    CAGATGAAGC TTCCTTCTTC TTCAACGTGG GGCCAGCAGT CCAATACAAC AGCATGTCAG
    TCCCAGGCCA CGCTGTCGTT GGCTGAAATC CAAAAACTAG AGGAAGAACG AGAACGGCAG
    CTTCGAGAAG AGCAAAGGCG CCAGCAGAGG GAGTTGATGA AAGCTCTTCA GCAGCAGCAG
    CAACAGCAAC AGCAGAAACT CTCAGGTTGG GGGAATGTCA GCAAACCTTC AGGTACCACG
    AAATCTCTTC TGGAGATCCA GCAGGAAGAG GCCAGGCAAA TGCAAAAGCA GCAGCAGCAG
    CAGCAGCAAC ACCAGCAACC AAACAGAGCT CGTAACAATA CGCATTCCAA CCTGCACACC
    AGCATTGGGA ATTCTGTTTG GGGCTCTATA AATACTGGTC CTCCTAACCA GTGGGCATCT
    GACCTAGTCA GTAGTATTTG GAGTAATGCT GACACTAAAA ACTCCAACAT GGGATTCTGG
    GATGATGCAG TGAAAGAGGT GGGACCTAGG AATTCAACAA ATAAAAATAA AAACAACGCC
    AGTCTCAGTA AATCTGTAGG TGTGTCTAAC CGGCAGAATA AGAAAGTAGA AGAAGAAGAA
    AAGTTGCTGA AGCTCTTTCA GGGAGTAAAT AAAGCCCAAG ATGGATTTAC GCAGTGGTGT
    GAACAGATGC TTCATGCCCT TAATACGGCA AATAACTTGG ATGTTCCCAC ATTTGTTTCT
    TTCCTGAAAG AAGTAGAATC TCCTTATGAG GTCCATGATT ATATCAGGGC CTATTTAGGA
    GATACTTCTG AGGCCAAGGA GTTTGCCAAG CAGTTCCTTG AGCGCCGTGC CAAACAGAAA
    GCCAACCAGC AGCGTCAGCA GCAGCAGCTG CCACAGCAGC AGCAGCAGCA GCCGCCACAG
    CAGCCGCCAC AGCAGCCACA ACAGCAGGAC TCTGTGTGGG GGATGAACCA CAGTACACTC
    CATTCAGTAT TTCAGACCAA TCAAAGCAAC AACCAACAAT CCAATTTTGA GGCTGTGCAG
    AGTGGCAAGA AGAAGAAAAA GCAGAAGATG GTCCGAGCAG ATCCCAGTTT ATTAGGATTT
    TCAGTCAATG CATCATCGGA GCGACTCAAC ATGGGTGAAA TCGAGACGTT GGATGACTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17213
    Clone ID Related Accession (Same CDS sequence) NM_001103147.1 , NM_001103147.2
    Accession Version NM_001103147.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3963bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-30
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform a
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG527686.1, BC146775.1, AC016692.9 and BE220828.1. On Jun 2, 2019 this sequence version replaced NM_001103147.1. Transcript Variant: This variant (1) encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC146775.1, AB014542.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1968540, SAMEA1968832 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGAAAGAAG
    AATGGATATT ATTGCATGTA CTCACCAGTT CTTTTGCTGG GTCAGCCTTT ATGCCAAGGG
    AGACCAAATT TTGAGGAAGG TGGACCAACA TCAGTAGGGA GAAAGCATGA ATTTATACGC
    TCAGAAAGTG AAAATTGGCG CATCTTTAGA GAGGAACAAA ATGGAGAAGA TGAAGATGGA
    GGTTGGCGAC TAGCTGGATC AAGGAGGGAT GGAGAGAGGT GGCGACCTCA CAGTCCTGAT
    GGCCCTCGTT CTGCAGGCTG GCGGGAACAC ATGGAACGAC GTCGGAGGTT TGAGTTTGAT
    TTTCGAGATA GAGATGATGA ACGGGGTTAC CGAAGGGTTC GCTCTGGCAG TGGGAGCATA
    GATGATGACA GGGATAGCTT GCCCGAATGG TGCTTAGAGG ATGCTGAAGA AGAAATGGGT
    ACATTTGACT CATCTGGAGC ATTCCTTTCT CTAAAAAAAG TACAGAAAGA GCCTATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GACGAAGGGG AGGAGTGCTC TGACTCTGAG
    GGTAGCCATA ATGAAGAGGC CAAAGAACCC GATAAGACAA ATAAGAAAGA AGGAGAGAAA
    ACAGATAGAG TAGGAGTTGC TAGTGAGGAA ACTCCCCAGA CCTCATCATC ATCTGCTAGA
    CCAGGTACTC CTTCAGACCA TCAGTCTCAG GAAGCATCAC AGTTTGAGAG GAAAGATGAA
    CCAAAAACTG AGCAAACGGA AAAAGCTGAA GAGGAGACTC GGATGGAAAA TAGTCTACCA
    GCCAAAGTGC CCAGCAGAGG GGATGAAATG GTTGCTGATG TCCAGCAGCC CCTGTCGCAG
    ATTCCTTCAG ATACAGCCTC TCCTCTTCTC ATACTTCCAC CTCCTGTTCC CAATCCTAGT
    CCTACTCTCC GGCCAGTTGA AACACCAGTT GTAGGTGCTC CTGGTATGGG CAGTGTTTCC
    ACAGAACCTG ATGATGAAGA AGGTCTCAAA CATTTGGAGC AGCAAGCTGA GAAAATGGTG
    GCTTATCTCC AAGACAGTGC ACTAGATGAT GAAAGATTGG CATCAAAACT GCAAGAGCAC
    AGAGCTAAAG GAGTGTCGAT TCCATTGATG CATGAAGCAA TGCAGAAGTG GTATTACAAA
    GATCCTCAGG GAGAAATTCA AGGTCCCTTC AATAATCAGG AGATGGCAGA ATGGTTTCAG
    GCGGGCTATT TTACTATGTC TTTATTGGTG AAGAGAGCGT GTGATGAAAG CTTCCAACCT
    CTTGGCGATA TCATGAAAAT GTGGGGAAGG GTTCCCTTTT CTCCAGGTCC AGCTCCCCCT
    CCTCATATGG GAGAGCTGGA CCAGGAACGA CTGACCAGGC AGCAAGAACT CACAGCCTTA
    TACCAGATGC AGCACCTGCA GTACCAGCAG TTTTTAATAC AACAACAATA TGCACAGGTT
    TTGGCCCAAC AGCAGAAAGC AGCACTGTCT TCCCAGCAGC AGCAGCAGTT GGCACTTCTT
    CTTCAACAGT TTCAGACCTT GAAGATGAGA ATATCTGATC AGAACATCAT TCCCTCAGTA
    ACTAGGTCTG TGTCCGTGCC AGATACTGGC TCTATCTGGG AGCTTCAGCC AACAGCTTCA
    CAGCCTACAG TTTGGGAAGG TGGTAGTGTA TGGGATCTTC CTCTGGACAC CACGACACCA
    GGCCCTGCCC TGGAACAGCT TCAGCAGCTA GAGAAGGCCA AAGCTGCAAA GCTAGAGCAA
    GAGAGAAGAG AGGCAGAAAT GAGGGCAAAA CGGGAAGAGG AAGAGCGAAA GAGGCAGGAA
    GAACTCCGAA GACAACAGGA GGAAATTCTT CGGCGACAGC AGGAAGAAGA AAGGAAAAGG
    CGAGAGGAAG AAGAACTTGC CCGAAGGAAA CAGGAAGAGG CTCTGCGTCG CCAGCGGGAG
    CAAGAAATTG CATTAAGGCG ACAGCGAGAA GAGGAAGAAA GACAGCAGCA AGAAGAAGCT
    CTTAGAAGAC TGGAAGAGAG GAGAAGAGAA GAGGAAGAAA GGCGGAAGCA GGAAGAATTG
    TTACGCAAAC AGGAAGAGGA GGCTGCAAAA TGGGCCCGGG AAGAAGAAGA AGCCCAGCGT
    CGATTAGAGG AGAACCGGCT GCGGATGGAA GAGGAGGCAG CCAGACTCCG GCATGAGGAA
    GAAGAACGGA AGAGAAAGGA GCTGGAGGTC CAGCGGCAGA AGGAGTTAAT GCGCCAGAGG
    CAGCAGCAGC AAGAGGCTCT CCGGAGGTTG CAGCAGCAGC AGCAGCAACA ACAGCTGGCG
    CAGATGAAGC TTCCTTCTTC TTCAACGTGG GGCCAGCAGT CCAATACAAC AGCATGTCAG
    TCCCAGGCCA CGCTGTCGTT GGCTGAAATC CAAAAACTAG AGGAAGAACG AGAACGGCAG
    CTTCGAGAAG AGCAAAGGCG CCAGCAGAGG GAGTTGATGA AAGCTCTTCA GCAGCAGCAG
    CAACAGCAAC AGCAGAAACT CTCAGGTTGG GGGAATGTCA GCAAACCTTC AGGTACCACG
    AAATCTCTTC TGGAGATCCA GCAGGAAGAG GCCAGGCAAA TGCAAAAGCA GCAGCAGCAG
    CAGCAGCAAC ACCAGCAACC AAACAGAGCT CGTAACAATA CGCATTCCAA CCTGCACACC
    AGCATTGGGA ATTCTGTTTG GGGCTCTATA AATACTGGTC CTCCTAACCA GTGGGCATCT
    GACCTAGTCA GTAGTATTTG GAGTAATGCT GACACTAAAA ACTCCAACAT GGGATTCTGG
    GATGATGCAG TGAAAGAGGT GGGACCTAGG AATTCAACAA ATAAAAATAA AAACAACGCC
    AGTCTCAGTA AATCTGTAGG TGTGTCTAAC CGGCAGAATA AGAAAGTAGA AGAAGAAGAA
    AAGTTGCTGA AGCTCTTTCA GGGAGTAAAT AAAGCCCAAG ATGGATTTAC GCAGTGGTGT
    GAACAGATGC TTCATGCCCT TAATACGGCA AATAACTTGG ATGTTCCCAC ATTTGTTTCT
    TTCCTGAAAG AAGTAGAATC TCCTTATGAG GTCCATGATT ATATCAGGGC CTATTTAGGA
    GATACTTCTG AGGCCAAGGA GTTTGCCAAG CAGTTCCTTG AGCGCCGTGC CAAACAGAAA
    GCCAACCAGC AGCGTCAGCA GCAGCAGCTG CCACAGCAGC AGCAGCAGCA GCCGCCACAG
    CAGCCGCCAC AGCAGCCACA ACAGCAGGAC TCTGTGTGGG GGATGAACCA CAGTACACTC
    CATTCAGTAT TTCAGACCAA TCAAAGCAAC AACCAACAAT CCAATTTTGA GGCTGTGCAG
    AGTGGCAAGA AGAAGAAAAA GCAGAAGATG GTCCGAGCAG ATCCCAGTTT ATTAGGATTT
    TCAGTCAATG CATCATCGGA GCGACTCAAC ATGGGTGAAA TCGAGACGTT GGATGACTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096617.1
    CDS242..4204
    Translation

    Target ORF information:

    RefSeq Version NM_001103147.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103147.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGAAAGAAG
    AATGGATATT ATTGCATGTA CTCACCAGTT CTTTTGCTGG GTCAGCCTTT ATGCCAAGGG
    AGACCAAATT TTGAGGAAGG TGGACCAACA TCAGTAGGGA GAAAGCATGA ATTTATACGC
    TCAGAAAGTG AAAATTGGCG CATCTTTAGA GAGGAACAAA ATGGAGAAGA TGAAGATGGA
    GGTTGGCGAC TAGCTGGATC AAGGAGGGAT GGAGAGAGGT GGCGACCTCA CAGTCCTGAT
    GGCCCTCGTT CTGCAGGCTG GCGGGAACAC ATGGAACGAC GTCGGAGGTT TGAGTTTGAT
    TTTCGAGATA GAGATGATGA ACGGGGTTAC CGAAGGGTTC GCTCTGGCAG TGGGAGCATA
    GATGATGACA GGGATAGCTT GCCCGAATGG TGCTTAGAGG ATGCTGAAGA AGAAATGGGT
    ACATTTGACT CATCTGGAGC ATTCCTTTCT CTAAAAAAAG TACAGAAAGA GCCTATTCCA
    GAAGAGCAGG AGATGGACTT CCGGCCTGTG GACGAAGGGG AGGAGTGCTC TGACTCTGAG
    GGTAGCCATA ATGAAGAGGC CAAAGAACCC GATAAGACAA ATAAGAAAGA AGGAGAGAAA
    ACAGATAGAG TAGGAGTTGC TAGTGAGGAA ACTCCCCAGA CCTCATCATC ATCTGCTAGA
    CCAGGTACTC CTTCAGACCA TCAGTCTCAG GAAGCATCAC AGTTTGAGAG GAAAGATGAA
    CCAAAAACTG AGCAAACGGA AAAAGCTGAA GAGGAGACTC GGATGGAAAA TAGTCTACCA
    GCCAAAGTGC CCAGCAGAGG GGATGAAATG GTTGCTGATG TCCAGCAGCC CCTGTCGCAG
    ATTCCTTCAG ATACAGCCTC TCCTCTTCTC ATACTTCCAC CTCCTGTTCC CAATCCTAGT
    CCTACTCTCC GGCCAGTTGA AACACCAGTT GTAGGTGCTC CTGGTATGGG CAGTGTTTCC
    ACAGAACCTG ATGATGAAGA AGGTCTCAAA CATTTGGAGC AGCAAGCTGA GAAAATGGTG
    GCTTATCTCC AAGACAGTGC ACTAGATGAT GAAAGATTGG CATCAAAACT GCAAGAGCAC
    AGAGCTAAAG GAGTGTCGAT TCCATTGATG CATGAAGCAA TGCAGAAGTG GTATTACAAA
    GATCCTCAGG GAGAAATTCA AGGTCCCTTC AATAATCAGG AGATGGCAGA ATGGTTTCAG
    GCGGGCTATT TTACTATGTC TTTATTGGTG AAGAGAGCGT GTGATGAAAG CTTCCAACCT
    CTTGGCGATA TCATGAAAAT GTGGGGAAGG GTTCCCTTTT CTCCAGGTCC AGCTCCCCCT
    CCTCATATGG GAGAGCTGGA CCAGGAACGA CTGACCAGGC AGCAAGAACT CACAGCCTTA
    TACCAGATGC AGCACCTGCA GTACCAGCAG TTTTTAATAC AACAACAATA TGCACAGGTT
    TTGGCCCAAC AGCAGAAAGC AGCACTGTCT TCCCAGCAGC AGCAGCAGTT GGCACTTCTT
    CTTCAACAGT TTCAGACCTT GAAGATGAGA ATATCTGATC AGAACATCAT TCCCTCAGTA
    ACTAGGTCTG TGTCCGTGCC AGATACTGGC TCTATCTGGG AGCTTCAGCC AACAGCTTCA
    CAGCCTACAG TTTGGGAAGG TGGTAGTGTA TGGGATCTTC CTCTGGACAC CACGACACCA
    GGCCCTGCCC TGGAACAGCT TCAGCAGCTA GAGAAGGCCA AAGCTGCAAA GCTAGAGCAA
    GAGAGAAGAG AGGCAGAAAT GAGGGCAAAA CGGGAAGAGG AAGAGCGAAA GAGGCAGGAA
    GAACTCCGAA GACAACAGGA GGAAATTCTT CGGCGACAGC AGGAAGAAGA AAGGAAAAGG
    CGAGAGGAAG AAGAACTTGC CCGAAGGAAA CAGGAAGAGG CTCTGCGTCG CCAGCGGGAG
    CAAGAAATTG CATTAAGGCG ACAGCGAGAA GAGGAAGAAA GACAGCAGCA AGAAGAAGCT
    CTTAGAAGAC TGGAAGAGAG GAGAAGAGAA GAGGAAGAAA GGCGGAAGCA GGAAGAATTG
    TTACGCAAAC AGGAAGAGGA GGCTGCAAAA TGGGCCCGGG AAGAAGAAGA AGCCCAGCGT
    CGATTAGAGG AGAACCGGCT GCGGATGGAA GAGGAGGCAG CCAGACTCCG GCATGAGGAA
    GAAGAACGGA AGAGAAAGGA GCTGGAGGTC CAGCGGCAGA AGGAGTTAAT GCGCCAGAGG
    CAGCAGCAGC AAGAGGCTCT CCGGAGGTTG CAGCAGCAGC AGCAGCAACA ACAGCTGGCG
    CAGATGAAGC TTCCTTCTTC TTCAACGTGG GGCCAGCAGT CCAATACAAC AGCATGTCAG
    TCCCAGGCCA CGCTGTCGTT GGCTGAAATC CAAAAACTAG AGGAAGAACG AGAACGGCAG
    CTTCGAGAAG AGCAAAGGCG CCAGCAGAGG GAGTTGATGA AAGCTCTTCA GCAGCAGCAG
    CAACAGCAAC AGCAGAAACT CTCAGGTTGG GGGAATGTCA GCAAACCTTC AGGTACCACG
    AAATCTCTTC TGGAGATCCA GCAGGAAGAG GCCAGGCAAA TGCAAAAGCA GCAGCAGCAG
    CAGCAGCAAC ACCAGCAACC AAACAGAGCT CGTAACAATA CGCATTCCAA CCTGCACACC
    AGCATTGGGA ATTCTGTTTG GGGCTCTATA AATACTGGTC CTCCTAACCA GTGGGCATCT
    GACCTAGTCA GTAGTATTTG GAGTAATGCT GACACTAAAA ACTCCAACAT GGGATTCTGG
    GATGATGCAG TGAAAGAGGT GGGACCTAGG AATTCAACAA ATAAAAATAA AAACAACGCC
    AGTCTCAGTA AATCTGTAGG TGTGTCTAAC CGGCAGAATA AGAAAGTAGA AGAAGAAGAA
    AAGTTGCTGA AGCTCTTTCA GGGAGTAAAT AAAGCCCAAG ATGGATTTAC GCAGTGGTGT
    GAACAGATGC TTCATGCCCT TAATACGGCA AATAACTTGG ATGTTCCCAC ATTTGTTTCT
    TTCCTGAAAG AAGTAGAATC TCCTTATGAG GTCCATGATT ATATCAGGGC CTATTTAGGA
    GATACTTCTG AGGCCAAGGA GTTTGCCAAG CAGTTCCTTG AGCGCCGTGC CAAACAGAAA
    GCCAACCAGC AGCGTCAGCA GCAGCAGCTG CCACAGCAGC AGCAGCAGCA GCCGCCACAG
    CAGCCGCCAC AGCAGCCACA ACAGCAGGAC TCTGTGTGGG GGATGAACCA CAGTACACTC
    CATTCAGTAT TTCAGACCAA TCAAAGCAAC AACCAACAAT CCAATTTTGA GGCTGTGCAG
    AGTGGCAAGA AGAAGAAAAA GCAGAAGATG GTCCGAGCAG ATCCCAGTTT ATTAGGATTT
    TCAGTCAATG CATCATCGGA GCGACTCAAC ATGGGTGAAA TCGAGACGTT GGATGACTAC
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17353
    Clone ID Related Accession (Same CDS sequence) NM_015575.3 , NM_015575.4 , NM_001103146.3 , NM_001103146.1 , NM_001103146.2
    Accession Version NM_001103146.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-11
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK001739.1, BX537885.1, BX538321.1, BC008072.2 and AC016692.9. On Dec 2, 2019 this sequence version replaced NM_001103146.2. Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##RefSeq-Attributes-START## RefSeq Select criteria :: based on manual assertion, conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096616.1
    CDS172..4071
    Translation

    Target ORF information:

    RefSeq Version NM_001103146.3
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103146.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17216
    Clone ID Related Accession (Same CDS sequence) NM_001103148.1 , NM_001103148.2
    Accession Version NM_001103148.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-17
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG527686.1, BX538172.1, BC146775.1, AC016692.9 and BE220828.1. Transcript Variant: This variant (4) lacks two alternate exons in the 5' UTR, and lacks an exon and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX538172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGGCTGGCGG
    GAACACATGG AACGACGTCG GAGGTTTGAG TTTGATTTTC GAGATAGAGA TGATGAACGG
    GGTTACCGAA GGGTTCGCTC TGGCAGTGGG AGCATAGATG ATGACAGGGA TAGCTTGCCC
    GAATGGTGCT TAGAGGATGC TGAAGAAGAA ATGGGTACAT TTGACTCATC TGGAGCATTC
    CTTTCTCTAA AAAAAGTACA GAAAGAGCCT ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGACG AAGGGGAGGA GTGCTCTGAC TCTGAGGGTA GCCATAATGA AGAGGCCAAA
    GAACCCGATA AGACAAATAA GAAAGAAGGA GAGAAAACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CTCCCCAGAC CTCATCATCA TCTGCTAGAC CAGGTACTCC TTCAGACCAT
    CAGTCTCAGG AAGCATCACA GTTTGAGAGG AAAGATGAAC CAAAAACTGA GCAAACGGAA
    AAAGCTGAAG AGGAGACTCG GATGGAAAAT AGTCTACCAG CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTGCTGATGT CCAGCAGCCC CTGTCGCAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC TCCTGTTCCC AATCCTAGTC CTACTCTCCG GCCAGTTGAA
    ACACCAGTTG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAACCTGA TGATGAAGAA
    GGTCTCAAAC ATTTGGAGCA GCAAGCTGAG AAAATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AAAGATTGGC ATCAAAACTG CAAGAGCACA GAGCTAAAGG AGTGTCGATT
    CCATTGATGC ATGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCAGAA TGGTTTCAGG CGGGCTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCGTG TGATGAAAGC TTCCAACCTC TTGGCGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTTTC TCCAGGTCCA GCTCCCCCTC CTCATATGGG AGAGCTGGAC
    CAGGAACGAC TGACCAGGCA GCAAGAACTC ACAGCCTTAT ACCAGATGCA GCACCTGCAG
    TACCAGCAGT TTTTAATACA ACAACAATAT GCACAGGTTT TGGCCCAACA GCAGAAAGCA
    GCACTGTCTT CCCAGCAGCA GCAGCAGTTG GCACTTCTTC TTCAACAGTT TCAGACCTTG
    AAGATGAGAA TATCTGATCA GAACATCATT CCCTCAGTAA CTAGGTCTGT GTCCGTGCCA
    GATACTGGCT CTATCTGGGA GCTTCAGCCA ACAGCTTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC TCTGGACACC ACGACACCAG GCCCTGCCCT GGAACAGCTT
    CAGCAGCTAG AGAAGGCCAA AGCTGCAAAG CTAGAGCAAG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA AGAGCGAAAG AGGCAGGAAG AACTCCGAAG ACAACAGGAG
    GAAATTCTTC GGCGACAGCA GGAAGAAGAA AGGAAAAGGC GAGAGGAAGA AGAACTTGCC
    CGAAGGAAAC AGGAAGAGGC TCTGCGTCGC CAGCGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAGCAGCAA GAAGAAGCTC TTAGAAGACT GGAAGAGAGG
    AGAAGAGAAG AGGAAGAAAG GCGGAAGCAG GAAGAATTGT TACGCAAACA GGAAGAGGAG
    GCTGCAAAAT GGGCCCGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGGATGGAAG AGGAGGCAGC CAGACTCCGG CATGAGGAAG AAGAACGGAA GAGAAAGGAG
    CTGGAGGTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAGCA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAGCA GCAGCAACAA CAGCTGGCGC AGATGAAGCT TCCTTCTTCT
    TCAACGTGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCCAC GCTGTCGTTG
    GCTGAAATCC AAAAACTAGA GGAAGAACGA GAACGGCAGC TTCGAGAAGA GCAAAGGCGC
    CAGCAGAGGG AGTTGATGAA AGCTCTTCAG CAGCAGCAGC AACAGCAACA GCAGAAACTC
    TCAGGTTGGG GGAATGTCAG CAAACCTTCA GGTACCACGA AATCTCTTCT GGAGATCCAG
    CAGGAAGAGG CCAGGCAAAT GCAAAAGCAG CAGCAGCAGC AGCAGCAACA CCAGCAACCA
    AACAGAGCTC GTAACAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC TCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATTTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCAACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACG CAGTGGTGTG AACAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACA TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATCAGGGCC TATTTAGGAG ATACTTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTTGA GCGCCGTGCC AAACAGAAAG CCAACCAGCA GCGTCAGCAG
    CAGCAGCTGC CACAGCAGCA GCAGCAGCAG CCGCCACAGC AGCCGCCACA GCAGCCACAA
    CAGCAGGACT CTGTGTGGGG GATGAACCAC AGTACACTCC ATTCAGTATT TCAGACCAAT
    CAAAGCAACA ACCAACAATC CAATTTTGAG GCTGTGCAGA GTGGCAAGAA GAAGAAAAAG
    CAGAAGATGG TCCGAGCAGA TCCCAGTTTA TTAGGATTTT CAGTCAATGC ATCATCGGAG
    CGACTCAACA TGGGTGAAAT CGAGACGTTG GATGACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096618.1
    CDS132..4013
    Translation

    Target ORF information:

    RefSeq Version NM_001103148.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103148.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGGCTGGCGG
    GAACACATGG AACGACGTCG GAGGTTTGAG TTTGATTTTC GAGATAGAGA TGATGAACGG
    GGTTACCGAA GGGTTCGCTC TGGCAGTGGG AGCATAGATG ATGACAGGGA TAGCTTGCCC
    GAATGGTGCT TAGAGGATGC TGAAGAAGAA ATGGGTACAT TTGACTCATC TGGAGCATTC
    CTTTCTCTAA AAAAAGTACA GAAAGAGCCT ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGACG AAGGGGAGGA GTGCTCTGAC TCTGAGGGTA GCCATAATGA AGAGGCCAAA
    GAACCCGATA AGACAAATAA GAAAGAAGGA GAGAAAACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CTCCCCAGAC CTCATCATCA TCTGCTAGAC CAGGTACTCC TTCAGACCAT
    CAGTCTCAGG AAGCATCACA GTTTGAGAGG AAAGATGAAC CAAAAACTGA GCAAACGGAA
    AAAGCTGAAG AGGAGACTCG GATGGAAAAT AGTCTACCAG CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTGCTGATGT CCAGCAGCCC CTGTCGCAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC TCCTGTTCCC AATCCTAGTC CTACTCTCCG GCCAGTTGAA
    ACACCAGTTG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAACCTGA TGATGAAGAA
    GGTCTCAAAC ATTTGGAGCA GCAAGCTGAG AAAATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AAAGATTGGC ATCAAAACTG CAAGAGCACA GAGCTAAAGG AGTGTCGATT
    CCATTGATGC ATGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCAGAA TGGTTTCAGG CGGGCTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCGTG TGATGAAAGC TTCCAACCTC TTGGCGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTTTC TCCAGGTCCA GCTCCCCCTC CTCATATGGG AGAGCTGGAC
    CAGGAACGAC TGACCAGGCA GCAAGAACTC ACAGCCTTAT ACCAGATGCA GCACCTGCAG
    TACCAGCAGT TTTTAATACA ACAACAATAT GCACAGGTTT TGGCCCAACA GCAGAAAGCA
    GCACTGTCTT CCCAGCAGCA GCAGCAGTTG GCACTTCTTC TTCAACAGTT TCAGACCTTG
    AAGATGAGAA TATCTGATCA GAACATCATT CCCTCAGTAA CTAGGTCTGT GTCCGTGCCA
    GATACTGGCT CTATCTGGGA GCTTCAGCCA ACAGCTTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC TCTGGACACC ACGACACCAG GCCCTGCCCT GGAACAGCTT
    CAGCAGCTAG AGAAGGCCAA AGCTGCAAAG CTAGAGCAAG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA AGAGCGAAAG AGGCAGGAAG AACTCCGAAG ACAACAGGAG
    GAAATTCTTC GGCGACAGCA GGAAGAAGAA AGGAAAAGGC GAGAGGAAGA AGAACTTGCC
    CGAAGGAAAC AGGAAGAGGC TCTGCGTCGC CAGCGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAGCAGCAA GAAGAAGCTC TTAGAAGACT GGAAGAGAGG
    AGAAGAGAAG AGGAAGAAAG GCGGAAGCAG GAAGAATTGT TACGCAAACA GGAAGAGGAG
    GCTGCAAAAT GGGCCCGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGGATGGAAG AGGAGGCAGC CAGACTCCGG CATGAGGAAG AAGAACGGAA GAGAAAGGAG
    CTGGAGGTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAGCA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAGCA GCAGCAACAA CAGCTGGCGC AGATGAAGCT TCCTTCTTCT
    TCAACGTGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCCAC GCTGTCGTTG
    GCTGAAATCC AAAAACTAGA GGAAGAACGA GAACGGCAGC TTCGAGAAGA GCAAAGGCGC
    CAGCAGAGGG AGTTGATGAA AGCTCTTCAG CAGCAGCAGC AACAGCAACA GCAGAAACTC
    TCAGGTTGGG GGAATGTCAG CAAACCTTCA GGTACCACGA AATCTCTTCT GGAGATCCAG
    CAGGAAGAGG CCAGGCAAAT GCAAAAGCAG CAGCAGCAGC AGCAGCAACA CCAGCAACCA
    AACAGAGCTC GTAACAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC TCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATTTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCAACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACG CAGTGGTGTG AACAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACA TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATCAGGGCC TATTTAGGAG ATACTTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTTGA GCGCCGTGCC AAACAGAAAG CCAACCAGCA GCGTCAGCAG
    CAGCAGCTGC CACAGCAGCA GCAGCAGCAG CCGCCACAGC AGCCGCCACA GCAGCCACAA
    CAGCAGGACT CTGTGTGGGG GATGAACCAC AGTACACTCC ATTCAGTATT TCAGACCAAT
    CAAAGCAACA ACCAACAATC CAATTTTGAG GCTGTGCAGA GTGGCAAGAA GAAGAAAAAG
    CAGAAGATGG TCCGAGCAGA TCCCAGTTTA TTAGGATTTT CAGTCAATGC ATCATCGGAG
    CGACTCAACA TGGGTGAAAT CGAGACGTTG GATGACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17216
    Clone ID Related Accession (Same CDS sequence) NM_001103148.1 , NM_001103148.2
    Accession Version NM_001103148.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3882bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-07-30
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform c
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG527686.1, BX538172.1, BC146775.1, AC016692.9 and BE220828.1. On Jun 2, 2019 this sequence version replaced NM_001103148.1. Transcript Variant: This variant (4) lacks two alternate exons in the 5' UTR, and lacks an exon and uses an alternate splice site in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (c) is shorter than isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BX538172.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1968540 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGGCTGGCGG
    GAACACATGG AACGACGTCG GAGGTTTGAG TTTGATTTTC GAGATAGAGA TGATGAACGG
    GGTTACCGAA GGGTTCGCTC TGGCAGTGGG AGCATAGATG ATGACAGGGA TAGCTTGCCC
    GAATGGTGCT TAGAGGATGC TGAAGAAGAA ATGGGTACAT TTGACTCATC TGGAGCATTC
    CTTTCTCTAA AAAAAGTACA GAAAGAGCCT ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGACG AAGGGGAGGA GTGCTCTGAC TCTGAGGGTA GCCATAATGA AGAGGCCAAA
    GAACCCGATA AGACAAATAA GAAAGAAGGA GAGAAAACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CTCCCCAGAC CTCATCATCA TCTGCTAGAC CAGGTACTCC TTCAGACCAT
    CAGTCTCAGG AAGCATCACA GTTTGAGAGG AAAGATGAAC CAAAAACTGA GCAAACGGAA
    AAAGCTGAAG AGGAGACTCG GATGGAAAAT AGTCTACCAG CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTGCTGATGT CCAGCAGCCC CTGTCGCAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC TCCTGTTCCC AATCCTAGTC CTACTCTCCG GCCAGTTGAA
    ACACCAGTTG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAACCTGA TGATGAAGAA
    GGTCTCAAAC ATTTGGAGCA GCAAGCTGAG AAAATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AAAGATTGGC ATCAAAACTG CAAGAGCACA GAGCTAAAGG AGTGTCGATT
    CCATTGATGC ATGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCAGAA TGGTTTCAGG CGGGCTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCGTG TGATGAAAGC TTCCAACCTC TTGGCGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTTTC TCCAGGTCCA GCTCCCCCTC CTCATATGGG AGAGCTGGAC
    CAGGAACGAC TGACCAGGCA GCAAGAACTC ACAGCCTTAT ACCAGATGCA GCACCTGCAG
    TACCAGCAGT TTTTAATACA ACAACAATAT GCACAGGTTT TGGCCCAACA GCAGAAAGCA
    GCACTGTCTT CCCAGCAGCA GCAGCAGTTG GCACTTCTTC TTCAACAGTT TCAGACCTTG
    AAGATGAGAA TATCTGATCA GAACATCATT CCCTCAGTAA CTAGGTCTGT GTCCGTGCCA
    GATACTGGCT CTATCTGGGA GCTTCAGCCA ACAGCTTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC TCTGGACACC ACGACACCAG GCCCTGCCCT GGAACAGCTT
    CAGCAGCTAG AGAAGGCCAA AGCTGCAAAG CTAGAGCAAG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA AGAGCGAAAG AGGCAGGAAG AACTCCGAAG ACAACAGGAG
    GAAATTCTTC GGCGACAGCA GGAAGAAGAA AGGAAAAGGC GAGAGGAAGA AGAACTTGCC
    CGAAGGAAAC AGGAAGAGGC TCTGCGTCGC CAGCGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAGCAGCAA GAAGAAGCTC TTAGAAGACT GGAAGAGAGG
    AGAAGAGAAG AGGAAGAAAG GCGGAAGCAG GAAGAATTGT TACGCAAACA GGAAGAGGAG
    GCTGCAAAAT GGGCCCGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGGATGGAAG AGGAGGCAGC CAGACTCCGG CATGAGGAAG AAGAACGGAA GAGAAAGGAG
    CTGGAGGTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAGCA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAGCA GCAGCAACAA CAGCTGGCGC AGATGAAGCT TCCTTCTTCT
    TCAACGTGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCCAC GCTGTCGTTG
    GCTGAAATCC AAAAACTAGA GGAAGAACGA GAACGGCAGC TTCGAGAAGA GCAAAGGCGC
    CAGCAGAGGG AGTTGATGAA AGCTCTTCAG CAGCAGCAGC AACAGCAACA GCAGAAACTC
    TCAGGTTGGG GGAATGTCAG CAAACCTTCA GGTACCACGA AATCTCTTCT GGAGATCCAG
    CAGGAAGAGG CCAGGCAAAT GCAAAAGCAG CAGCAGCAGC AGCAGCAACA CCAGCAACCA
    AACAGAGCTC GTAACAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC TCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATTTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCAACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACG CAGTGGTGTG AACAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACA TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATCAGGGCC TATTTAGGAG ATACTTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTTGA GCGCCGTGCC AAACAGAAAG CCAACCAGCA GCGTCAGCAG
    CAGCAGCTGC CACAGCAGCA GCAGCAGCAG CCGCCACAGC AGCCGCCACA GCAGCCACAA
    CAGCAGGACT CTGTGTGGGG GATGAACCAC AGTACACTCC ATTCAGTATT TCAGACCAAT
    CAAAGCAACA ACCAACAATC CAATTTTGAG GCTGTGCAGA GTGGCAAGAA GAAGAAAAAG
    CAGAAGATGG TCCGAGCAGA TCCCAGTTTA TTAGGATTTT CAGTCAATGC ATCATCGGAG
    CGACTCAACA TGGGTGAAAT CGAGACGTTG GATGACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096618.1
    CDS106..3987
    Translation

    Target ORF information:

    RefSeq Version NM_001103148.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103148.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGGCTGGCGG
    GAACACATGG AACGACGTCG GAGGTTTGAG TTTGATTTTC GAGATAGAGA TGATGAACGG
    GGTTACCGAA GGGTTCGCTC TGGCAGTGGG AGCATAGATG ATGACAGGGA TAGCTTGCCC
    GAATGGTGCT TAGAGGATGC TGAAGAAGAA ATGGGTACAT TTGACTCATC TGGAGCATTC
    CTTTCTCTAA AAAAAGTACA GAAAGAGCCT ATTCCAGAAG AGCAGGAGAT GGACTTCCGG
    CCTGTGGACG AAGGGGAGGA GTGCTCTGAC TCTGAGGGTA GCCATAATGA AGAGGCCAAA
    GAACCCGATA AGACAAATAA GAAAGAAGGA GAGAAAACAG ATAGAGTAGG AGTTGAAGCT
    AGTGAGGAAA CTCCCCAGAC CTCATCATCA TCTGCTAGAC CAGGTACTCC TTCAGACCAT
    CAGTCTCAGG AAGCATCACA GTTTGAGAGG AAAGATGAAC CAAAAACTGA GCAAACGGAA
    AAAGCTGAAG AGGAGACTCG GATGGAAAAT AGTCTACCAG CCAAAGTGCC CAGCAGAGGG
    GATGAAATGG TTGCTGATGT CCAGCAGCCC CTGTCGCAGA TTCCTTCAGA TACAGCCTCT
    CCTCTTCTCA TACTTCCACC TCCTGTTCCC AATCCTAGTC CTACTCTCCG GCCAGTTGAA
    ACACCAGTTG TAGGTGCTCC TGGTATGGGC AGTGTTTCCA CAGAACCTGA TGATGAAGAA
    GGTCTCAAAC ATTTGGAGCA GCAAGCTGAG AAAATGGTGG CTTATCTCCA AGACAGTGCA
    CTAGATGATG AAAGATTGGC ATCAAAACTG CAAGAGCACA GAGCTAAAGG AGTGTCGATT
    CCATTGATGC ATGAAGCAAT GCAGAAGTGG TATTACAAAG ATCCTCAGGG AGAAATTCAA
    GGTCCCTTCA ATAATCAGGA GATGGCAGAA TGGTTTCAGG CGGGCTATTT TACTATGTCT
    TTATTGGTGA AGAGAGCGTG TGATGAAAGC TTCCAACCTC TTGGCGATAT CATGAAAATG
    TGGGGAAGGG TTCCCTTTTC TCCAGGTCCA GCTCCCCCTC CTCATATGGG AGAGCTGGAC
    CAGGAACGAC TGACCAGGCA GCAAGAACTC ACAGCCTTAT ACCAGATGCA GCACCTGCAG
    TACCAGCAGT TTTTAATACA ACAACAATAT GCACAGGTTT TGGCCCAACA GCAGAAAGCA
    GCACTGTCTT CCCAGCAGCA GCAGCAGTTG GCACTTCTTC TTCAACAGTT TCAGACCTTG
    AAGATGAGAA TATCTGATCA GAACATCATT CCCTCAGTAA CTAGGTCTGT GTCCGTGCCA
    GATACTGGCT CTATCTGGGA GCTTCAGCCA ACAGCTTCAC AGCCTACAGT TTGGGAAGGT
    GGTAGTGTAT GGGATCTTCC TCTGGACACC ACGACACCAG GCCCTGCCCT GGAACAGCTT
    CAGCAGCTAG AGAAGGCCAA AGCTGCAAAG CTAGAGCAAG AGAGAAGAGA GGCAGAAATG
    AGGGCAAAAC GGGAAGAGGA AGAGCGAAAG AGGCAGGAAG AACTCCGAAG ACAACAGGAG
    GAAATTCTTC GGCGACAGCA GGAAGAAGAA AGGAAAAGGC GAGAGGAAGA AGAACTTGCC
    CGAAGGAAAC AGGAAGAGGC TCTGCGTCGC CAGCGGGAGC AAGAAATTGC ATTAAGGCGA
    CAGCGAGAAG AGGAAGAAAG ACAGCAGCAA GAAGAAGCTC TTAGAAGACT GGAAGAGAGG
    AGAAGAGAAG AGGAAGAAAG GCGGAAGCAG GAAGAATTGT TACGCAAACA GGAAGAGGAG
    GCTGCAAAAT GGGCCCGGGA AGAAGAAGAA GCCCAGCGTC GATTAGAGGA GAACCGGCTG
    CGGATGGAAG AGGAGGCAGC CAGACTCCGG CATGAGGAAG AAGAACGGAA GAGAAAGGAG
    CTGGAGGTCC AGCGGCAGAA GGAGTTAATG CGCCAGAGGC AGCAGCAGCA AGAGGCTCTC
    CGGAGGTTGC AGCAGCAGCA GCAGCAACAA CAGCTGGCGC AGATGAAGCT TCCTTCTTCT
    TCAACGTGGG GCCAGCAGTC CAATACAACA GCATGTCAGT CCCAGGCCAC GCTGTCGTTG
    GCTGAAATCC AAAAACTAGA GGAAGAACGA GAACGGCAGC TTCGAGAAGA GCAAAGGCGC
    CAGCAGAGGG AGTTGATGAA AGCTCTTCAG CAGCAGCAGC AACAGCAACA GCAGAAACTC
    TCAGGTTGGG GGAATGTCAG CAAACCTTCA GGTACCACGA AATCTCTTCT GGAGATCCAG
    CAGGAAGAGG CCAGGCAAAT GCAAAAGCAG CAGCAGCAGC AGCAGCAACA CCAGCAACCA
    AACAGAGCTC GTAACAATAC GCATTCCAAC CTGCACACCA GCATTGGGAA TTCTGTTTGG
    GGCTCTATAA ATACTGGTCC TCCTAACCAG TGGGCATCTG ACCTAGTCAG TAGTATTTGG
    AGTAATGCTG ACACTAAAAA CTCCAACATG GGATTCTGGG ATGATGCAGT GAAAGAGGTG
    GGACCTAGGA ATTCAACAAA TAAAAATAAA AACAACGCCA GTCTCAGTAA ATCTGTAGGT
    GTGTCTAACC GGCAGAATAA GAAAGTAGAA GAAGAAGAAA AGTTGCTGAA GCTCTTTCAG
    GGAGTAAATA AAGCCCAAGA TGGATTTACG CAGTGGTGTG AACAGATGCT TCATGCCCTT
    AATACGGCAA ATAACTTGGA TGTTCCCACA TTTGTTTCTT TCCTGAAAGA AGTAGAATCT
    CCTTATGAGG TCCATGATTA TATCAGGGCC TATTTAGGAG ATACTTCTGA GGCCAAGGAG
    TTTGCCAAGC AGTTCCTTGA GCGCCGTGCC AAACAGAAAG CCAACCAGCA GCGTCAGCAG
    CAGCAGCTGC CACAGCAGCA GCAGCAGCAG CCGCCACAGC AGCCGCCACA GCAGCCACAA
    CAGCAGGACT CTGTGTGGGG GATGAACCAC AGTACACTCC ATTCAGTATT TCAGACCAAT
    CAAAGCAACA ACCAACAATC CAATTTTGAG GCTGTGCAGA GTGGCAAGAA GAAGAAAAAG
    CAGAAGATGG TCCGAGCAGA TCCCAGTTTA TTAGGATTTT CAGTCAATGC ATCATCGGAG
    CGACTCAACA TGGGTGAAAT CGAGACGTTG GATGACTACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17353
    Clone ID Related Accession (Same CDS sequence) NM_015575.3 , NM_015575.4 , NM_001103146.3 , NM_001103146.1 , NM_001103146.2
    Accession Version NM_015575.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-17
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG527686.1, AY176045.1, BC146775.1, AC016692.9 and BE220828.1. This sequence is a reference standard in the RefSeqGene project. On Sep 5, 2007 this sequence version replaced NM_015575.2. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY176045.1, SRR1803615.67605.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056390.2
    CDS338..4237
    Translation

    Target ORF information:

    RefSeq Version NM_015575.3
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015575.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17353
    Clone ID Related Accession (Same CDS sequence) NM_015575.3 , NM_015575.4 , NM_001103146.3 , NM_001103146.1 , NM_001103146.2
    Accession Version NM_015575.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-08-21
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AY176045.1, BC146775.1, AC016692.9 and BE220828.1. This sequence is a reference standard in the RefSeqGene project. On Jun 2, 2019 this sequence version replaced NM_015575.3. Transcript Variant: This variant (2) contains an alternate exon in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY176045.1, SRR1803614.3524.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA1965299, SAMEA1966682 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_056390.2
    CDS312..4211
    Translation

    Target ORF information:

    RefSeq Version NM_015575.4
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_015575.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17353
    Clone ID Related Accession (Same CDS sequence) NM_015575.3 , NM_015575.4 , NM_001103146.3 , NM_001103146.1 , NM_001103146.2
    Accession Version NM_001103146.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-02-17
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BG527686.1, DC346582.1, BX538321.1, BC146775.1, AC016692.9 and BE220828.1. Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.177494.1, SRR1660809.37210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096616.1
    CDS198..4097
    Translation

    Target ORF information:

    RefSeq Version NM_001103146.1
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103146.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OHu17353
    Clone ID Related Accession (Same CDS sequence) NM_015575.3 , NM_015575.4 , NM_001103146.3 , NM_001103146.1 , NM_001103146.2
    Accession Version NM_001103146.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-06-01
    Organism Homo sapiens(human)
    Product GRB10-interacting GYF protein 2 isoform b
    Comment Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DC346582.1, BX538321.1, BC146775.1, AC016692.9 and BE220828.1. On Jun 2, 2019 this sequence version replaced NM_001103146.1. Transcript Variant: This variant (3) lacks an alternate exon in the 5' UTR and lacks an exon in the coding region, but maintains the reading frame, compared to variant 1. The encoded isoform (b) is shorter than isoform a. Both variants 2 and 3 encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1660803.177494.1, SRR1660809.37210.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## RefSeq Select criteria :: based on conservation, expression ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001096616.1
    CDS172..4071
    Translation

    Target ORF information:

    RefSeq Version NM_001103146.2
    Organism Homo sapiens(human)
    Definition Homo sapiens GRB10 interacting GYF protein 2 (GIGYF2), transcript variant 3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001103146.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGT 
    GGTGGGAGTA TTACATCCCC TCCTCTTTCT CCAGCATTGC CGAAGTATAA ATTAGCAGAT
    TATCGTTACG GCAGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAACAA GATACCTTCA
    GACCTTCTGG ATAAAGAATT TCTGCCTATC CTCCAGGAGG AACCCCTTCC ACCATTGGCT
    CTGGTACCCT TTACAGAAGA AGAACAGAGA AACTTTTCCA TGTCTGTAAA TAGTGCTGCT
    GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACAGTGG TGGGGGCTCC TAGAGGTCGA
    AGTTCTTCAA GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGTTTT
    GATGAAGTAG AGGGTGTTTT TGGTCGAGGA GGTGGCAGAG AAATGCATAG ATCGCAGAGC
    TGGGAGGAAA GGGGTGACAG ACGTTTTGAA AAACCAGGAC GAAAAGATGT AGGGAGACCA
    AATTTTGAGG AAGGTGGACC AACATCAGTA GGGAGAAAGC ATGAATTTAT ACGCTCAGAA
    AGTGAAAATT GGCGCATCTT TAGAGAGGAA CAAAATGGAG AAGATGAAGA TGGAGGTTGG
    CGACTAGCTG GATCAAGGAG GGATGGAGAG AGGTGGCGAC CTCACAGTCC TGATGGCCCT
    CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGTCGGA GGTTTGAGTT TGATTTTCGA
    GATAGAGATG ATGAACGGGG TTACCGAAGG GTTCGCTCTG GCAGTGGGAG CATAGATGAT
    GACAGGGATA GCTTGCCCGA ATGGTGCTTA GAGGATGCTG AAGAAGAAAT GGGTACATTT
    GACTCATCTG GAGCATTCCT TTCTCTAAAA AAAGTACAGA AAGAGCCTAT TCCAGAAGAG
    CAGGAGATGG ACTTCCGGCC TGTGGACGAA GGGGAGGAGT GCTCTGACTC TGAGGGTAGC
    CATAATGAAG AGGCCAAAGA ACCCGATAAG ACAAATAAGA AAGAAGGAGA GAAAACAGAT
    AGAGTAGGAG TTGAAGCTAG TGAGGAAACT CCCCAGACCT CATCATCATC TGCTAGACCA
    GGTACTCCTT CAGACCATCA GTCTCAGGAA GCATCACAGT TTGAGAGGAA AGATGAACCA
    AAAACTGAGC AAACGGAAAA AGCTGAAGAG GAGACTCGGA TGGAAAATAG TCTACCAGCC
    AAAGTGCCCA GCAGAGGGGA TGAAATGGTT GCTGATGTCC AGCAGCCCCT GTCGCAGATT
    CCTTCAGATA CAGCCTCTCC TCTTCTCATA CTTCCACCTC CTGTTCCCAA TCCTAGTCCT
    ACTCTCCGGC CAGTTGAAAC ACCAGTTGTA GGTGCTCCTG GTATGGGCAG TGTTTCCACA
    GAACCTGATG ATGAAGAAGG TCTCAAACAT TTGGAGCAGC AAGCTGAGAA AATGGTGGCT
    TATCTCCAAG ACAGTGCACT AGATGATGAA AGATTGGCAT CAAAACTGCA AGAGCACAGA
    GCTAAAGGAG TGTCGATTCC ATTGATGCAT GAAGCAATGC AGAAGTGGTA TTACAAAGAT
    CCTCAGGGAG AAATTCAAGG TCCCTTCAAT AATCAGGAGA TGGCAGAATG GTTTCAGGCG
    GGCTATTTTA CTATGTCTTT ATTGGTGAAG AGAGCGTGTG ATGAAAGCTT CCAACCTCTT
    GGCGATATCA TGAAAATGTG GGGAAGGGTT CCCTTTTCTC CAGGTCCAGC TCCCCCTCCT
    CATATGGGAG AGCTGGACCA GGAACGACTG ACCAGGCAGC AAGAACTCAC AGCCTTATAC
    CAGATGCAGC ACCTGCAGTA CCAGCAGTTT TTAATACAAC AACAATATGC ACAGGTTTTG
    GCCCAACAGC AGAAAGCAGC ACTGTCTTCC CAGCAGCAGC AGCAGTTGGC ACTTCTTCTT
    CAACAGTTTC AGACCTTGAA GATGAGAATA TCTGATCAGA ACATCATTCC CTCAGTAACT
    AGGTCTGTGT CCGTGCCAGA TACTGGCTCT ATCTGGGAGC TTCAGCCAAC AGCTTCACAG
    CCTACAGTTT GGGAAGGTGG TAGTGTATGG GATCTTCCTC TGGACACCAC GACACCAGGC
    CCTGCCCTGG AACAGCTTCA GCAGCTAGAG AAGGCCAAAG CTGCAAAGCT AGAGCAAGAG
    AGAAGAGAGG CAGAAATGAG GGCAAAACGG GAAGAGGAAG AGCGAAAGAG GCAGGAAGAA
    CTCCGAAGAC AACAGGAGGA AATTCTTCGG CGACAGCAGG AAGAAGAAAG GAAAAGGCGA
    GAGGAAGAAG AACTTGCCCG AAGGAAACAG GAAGAGGCTC TGCGTCGCCA GCGGGAGCAA
    GAAATTGCAT TAAGGCGACA GCGAGAAGAG GAAGAAAGAC AGCAGCAAGA AGAAGCTCTT
    AGAAGACTGG AAGAGAGGAG AAGAGAAGAG GAAGAAAGGC GGAAGCAGGA AGAATTGTTA
    CGCAAACAGG AAGAGGAGGC TGCAAAATGG GCCCGGGAAG AAGAAGAAGC CCAGCGTCGA
    TTAGAGGAGA ACCGGCTGCG GATGGAAGAG GAGGCAGCCA GACTCCGGCA TGAGGAAGAA
    GAACGGAAGA GAAAGGAGCT GGAGGTCCAG CGGCAGAAGG AGTTAATGCG CCAGAGGCAG
    CAGCAGCAAG AGGCTCTCCG GAGGTTGCAG CAGCAGCAGC AGCAACAACA GCTGGCGCAG
    ATGAAGCTTC CTTCTTCTTC AACGTGGGGC CAGCAGTCCA ATACAACAGC ATGTCAGTCC
    CAGGCCACGC TGTCGTTGGC TGAAATCCAA AAACTAGAGG AAGAACGAGA ACGGCAGCTT
    CGAGAAGAGC AAAGGCGCCA GCAGAGGGAG TTGATGAAAG CTCTTCAGCA GCAGCAGCAA
    CAGCAACAGC AGAAACTCTC AGGTTGGGGG AATGTCAGCA AACCTTCAGG TACCACGAAA
    TCTCTTCTGG AGATCCAGCA GGAAGAGGCC AGGCAAATGC AAAAGCAGCA GCAGCAGCAG
    CAGCAACACC AGCAACCAAA CAGAGCTCGT AACAATACGC ATTCCAACCT GCACACCAGC
    ATTGGGAATT CTGTTTGGGG CTCTATAAAT ACTGGTCCTC CTAACCAGTG GGCATCTGAC
    CTAGTCAGTA GTATTTGGAG TAATGCTGAC ACTAAAAACT CCAACATGGG ATTCTGGGAT
    GATGCAGTGA AAGAGGTGGG ACCTAGGAAT TCAACAAATA AAAATAAAAA CAACGCCAGT
    CTCAGTAAAT CTGTAGGTGT GTCTAACCGG CAGAATAAGA AAGTAGAAGA AGAAGAAAAG
    TTGCTGAAGC TCTTTCAGGG AGTAAATAAA GCCCAAGATG GATTTACGCA GTGGTGTGAA
    CAGATGCTTC ATGCCCTTAA TACGGCAAAT AACTTGGATG TTCCCACATT TGTTTCTTTC
    CTGAAAGAAG TAGAATCTCC TTATGAGGTC CATGATTATA TCAGGGCCTA TTTAGGAGAT
    ACTTCTGAGG CCAAGGAGTT TGCCAAGCAG TTCCTTGAGC GCCGTGCCAA ACAGAAAGCC
    AACCAGCAGC GTCAGCAGCA GCAGCTGCCA CAGCAGCAGC AGCAGCAGCC GCCACAGCAG
    CCGCCACAGC AGCCACAACA GCAGGACTCT GTGTGGGGGA TGAACCACAG TACACTCCAT
    TCAGTATTTC AGACCAATCA AAGCAACAAC CAACAATCCA ATTTTGAGGC TGTGCAGAGT
    GGCAAGAAGA AGAAAAAGCA GAAGATGGTC CGAGCAGATC CCAGTTTATT AGGATTTTCA
    GTCAATGCAT CATCGGAGCG ACTCAACATG GGTGAAATCG AGACGTTGGA TGACTACTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.