GIGYF2 cDNA ORF clone, Bos taurus(cattle)

Gene
Clones
gRNAs

Summary

Gene Symbol	GIGYF2
Entrez Gene ID	506913
Full Name	GRB10 interacting GYF protein 2
Synonyms	TNRC15
General protein information	Preferred Names GRB10 interacting GYF protein 2 Names PERQ amino acid-rich with GYF domain-containing protein 2 trinucleotide repeat containing 15
Gene Type	protein-coding
Organism	Bos taurus(cattle)

mRNA and Protein(s)

mRNA	Protein	Name
XM_024986639.1	XP_024842407.1	GRB10-interacting GYF protein 2 isoform X1
XM_024986647.1	XP_024842415.1	GRB10-interacting GYF protein 2 isoform X2
NM_001101877.1	NP_001095347.1	GRB10-interacting GYF protein 2
XM_024986645.1	XP_024842413.1	GRB10-interacting GYF protein 2 isoform X1
XM_024986648.1	XP_024842416.1	GRB10-interacting GYF protein 2 isoform X3
XM_024986654.1	XP_024842422.1	GRB10-interacting GYF protein 2 isoform X4
XM_024986656.1	XP_024842424.1	GRB10-interacting GYF protein 2 isoform X5

Pathways from BioSystems

Homologies

	GIGYF2	XP_001114252.2
Mus musculus (house mouse)	Gigyf2	NP_666224.3
Gallus gallus (chicken)	GIGYF2	NP_001239057.1
Danio rerio (zebrafish)	gigfy2	NP_001025332.1
Xenopus tropicalis (tropical clawed frog)	gigyf2	XP_002933400.2
Rattus norvegicus (Norway rat)	Gigyf2	XP_006245571.1
Homo sapiens (human)	GIGYF2	NP_001096616.1
Canis lupus familiaris (dog)	GIGYF2	XP_861928.1
Pan troglodytes (chimpanzee)	GIGYF2	XP_001148316.1
Bos taurus (cattle)	GIGYF2	NP_001095347.1

Gene Ontology
Bibliography

Related articles in PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS, Mar?ais G, Roberts M, Subramanian P, Yorke JA, Salzberg SL
Genome biology10(4)R42(2009)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following GIGYF2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GIGYF2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa232605	XM_024986639.1 Latest version!	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa232605	XM_024986645.1 Latest version!	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa232606	XM_024986647.1 Latest version!	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa232607	XM_024986648.1 Latest version!	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa232608	XM_024986654.1 Latest version!	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa232609	XM_024986656.1 Latest version!	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa38871	NM_001101877.1 Latest version!	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$713.30 $1019.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GIGYF2 ( XM_024986639.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842407.1 Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OBa232605
Clone ID Related Accession (Same CDS sequence)	XM_024986639.1 , XM_024986645.1
Accession Version	XM_024986639.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
GACTCTGTGT GGGGCATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAGACC 
AACAACCAAC AATCCAGTTT TGAAGCTGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG 
ATGGTCCGAG CAGATCCTAG TCTATTAGGG TTTTCAGTCA ACGCTTCCTC GGAGCGACTC 
AATATGGGTG AGATCGAAAC TGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842407.1
CDS	219..4094
Translation	MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGRSSSRGRGRGRGECGFYQRSFDEVEGVFVRGGGREMHRSQSWEERGDRRFDKPGRKDVGRPNFEEGGPVSVGRKHEFIRSESGNWRIFREEQNGEDEDGGWRLTGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEECSDSEGSHNEEAKEPDKTNKKEAEKTDRTGVEASEETTQTSSSSARPGSPSDQPQEAPQFERKEESKTEQMEKAEEESRTENSLSTKVSSRGDEVLPAVQQPLSQIPSDTASPLLILPPPVPSPSPALRPVETPVVGAPGMGSVPTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQALKMRISDQNIIPSVTRSVSVPDTGSIWELQPAPSQPTVWEGGSVWDLPLDPATPAPALEQLQQMEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELELQRQKELLRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPAGTAKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSSADTKNSNMGFWDDAVKEVGPRNSTNKNKNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQRQQQQQQQQQQQQQQQQQQQDSVWGMNHSALHSVFQTNQTNNQQSSFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVDDY

Target ORF information:

RefSeq Version	XM_024986639.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986639.1

ORF Insert Sequence:

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
GACTCTGTGT GGGGCATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAGACC 
AACAACCAAC AATCCAGTTT TGAAGCTGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG 
ATGGTCCGAG CAGATCCTAG TCTATTAGGG TTTTCAGTCA ACGCTTCCTC GGAGCGACTC 
AATATGGGTG AGATCGAAAC TGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_024986645.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842413.1 Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa232605
Clone ID Related Accession (Same CDS sequence)	XM_024986639.1 , XM_024986645.1
Accession Version	XM_024986645.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
GACTCTGTGT GGGGCATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAGACC 
AACAACCAAC AATCCAGTTT TGAAGCTGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG 
ATGGTCCGAG CAGATCCTAG TCTATTAGGG TTTTCAGTCA ACGCTTCCTC GGAGCGACTC 
AATATGGGTG AGATCGAAAC TGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842413.1
CDS	1680..5555
Translation	MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGRSSSRGRGRGRGECGFYQRSFDEVEGVFVRGGGREMHRSQSWEERGDRRFDKPGRKDVGRPNFEEGGPVSVGRKHEFIRSESGNWRIFREEQNGEDEDGGWRLTGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEECSDSEGSHNEEAKEPDKTNKKEAEKTDRTGVEASEETTQTSSSSARPGSPSDQPQEAPQFERKEESKTEQMEKAEEESRTENSLSTKVSSRGDEVLPAVQQPLSQIPSDTASPLLILPPPVPSPSPALRPVETPVVGAPGMGSVPTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQALKMRISDQNIIPSVTRSVSVPDTGSIWELQPAPSQPTVWEGGSVWDLPLDPATPAPALEQLQQMEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELELQRQKELLRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPAGTAKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSSADTKNSNMGFWDDAVKEVGPRNSTNKNKNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQRQQQQQQQQQQQQQQQQQQQDSVWGMNHSALHSVFQTNQTNNQQSSFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVDDY

Target ORF information:

RefSeq Version	XM_024986645.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986645.1

ORF Insert Sequence:

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
GACTCTGTGT GGGGCATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAGACC 
AACAACCAAC AATCCAGTTT TGAAGCTGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG 
ATGGTCCGAG CAGATCCTAG TCTATTAGGG TTTTCAGTCA ACGCTTCCTC GGAGCGACTC 
AATATGGGTG AGATCGAAAC TGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_024986647.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842415.1 Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.

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CloneID	OBa232606
Clone ID Related Accession (Same CDS sequence)	XM_024986647.1
Accession Version	XM_024986647.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3873bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC 
TCTGTGTGGG GCATGAACCA CAGTGCACTC CATTCAGTAT TTCAGACCAA TCAGACCAAC 
AACCAACAAT CCAGTTTTGA AGCTGTACAA AGTGGCAAGA AGAAGAAAAA GCAGAAGATG 
GTCCGAGCAG ATCCTAGTCT ATTAGGGTTT TCAGTCAACG CTTCCTCGGA GCGACTCAAT 
ATGGGTGAGA TCGAAACTGT GGATGACTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842415.1
CDS	276..4148
Translation	MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGRSSSRGRGRGRGECGFYQRSFDEVEGVFVRGGGREMHRSQSWEERGDRRFDKPGRKDVGRPNFEEGGPVSVGRKHEFIRSESGNWRIFREEQNGEDEDGGWRLTGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEECSDSEGSHNEEAKEPDKTNKKEAEKTDRTGVEASEETTQTSSSSARPGSPSDQPQEAPQFERKEESKTEQMEKAEEESRTENSLSTKVSSRGDEVLPAVQQPLSQIPSDTASPLLILPPPVPSPSPALRPVETPVVGAPGMGSVPTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQALKMRISDQNIIPSVTRSVSVPDTGSIWELQPAPSQPTVWEGGSVWDLPLDPATPAPALEQLQQMEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELELQRQKELLRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPAGTAKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSSADTKNSNMGFWDDAVKEVGPRNSTNKNKNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQRQQQQQQQQQQQQQQQQQQDSVWGMNHSALHSVFQTNQTNNQQSSFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVDDY

Target ORF information:

RefSeq Version	XM_024986647.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986647.1

ORF Insert Sequence:

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC 
TCTGTGTGGG GCATGAACCA CAGTGCACTC CATTCAGTAT TTCAGACCAA TCAGACCAAC 
AACCAACAAT CCAGTTTTGA AGCTGTACAA AGTGGCAAGA AGAAGAAAAA GCAGAAGATG 
GTCCGAGCAG ATCCTAGTCT ATTAGGGTTT TCAGTCAACG CTTCCTCGGA GCGACTCAAT 
ATGGGTGAGA TCGAAACTGT GGATGACTAC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_024986648.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842416.1 Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OBa232607
Clone ID Related Accession (Same CDS sequence)	XM_024986648.1
Accession Version	XM_024986648.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3867bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GGACTCTGTG 
TGGGGCATGA ACCACAGTGC ACTCCATTCA GTATTTCAGA CCAATCAGAC CAACAACCAA 
CAATCCAGTT TTGAAGCTGT ACAAAGTGGC AAGAAGAAGA AAAAGCAGAA GATGGTCCGA 
GCAGATCCTA GTCTATTAGG GTTTTCAGTC AACGCTTCCT CGGAGCGACT CAATATGGGT 
GAGATCGAAA CTGTGGATGA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842416.1
CDS	276..4142
Translation	MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGRSSSRGRGRGRGECGFYQRSFDEVEGVFVRGGGREMHRSQSWEERGDRRFDKPGRKDVGRPNFEEGGPVSVGRKHEFIRSESGNWRIFREEQNGEDEDGGWRLTGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEECSDSEGSHNEEAKEPDKTNKKEAEKTDRTGVEASEETTQTSSSSARPGSPSDQPQEAPQFERKEESKTEQMEKAEEESRTENSLSTKVSSRGDEVLPAVQQPLSQIPSDTASPLLILPPPVPSPSPALRPVETPVVGAPGMGSVPTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQALKMRISDQNIIPSVTRSVSVPDTGSIWELQPAPSQPTVWEGGSVWDLPLDPATPAPALEQLQQMEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELELQRQKELLRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPAGTAKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSSADTKNSNMGFWDDAVKEVGPRNSTNKNKNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQRQQQQQQQQQQQQQQQQDSVWGMNHSALHSVFQTNQTNNQQSSFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVDDY

Target ORF information:

RefSeq Version	XM_024986648.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986648.1

ORF Insert Sequence:

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCGACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GGACTCTGTG 
TGGGGCATGA ACCACAGTGC ACTCCATTCA GTATTTCAGA CCAATCAGAC CAACAACCAA 
CAATCCAGTT TTGAAGCTGT ACAAAGTGGC AAGAAGAAGA AAAAGCAGAA GATGGTCCGA 
GCAGATCCTA GTCTATTAGG GTTTTCAGTC AACGCTTCCT CGGAGCGACT CAATATGGGT 
GAGATCGAAA CTGTGGATGA CTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_024986654.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842422.1 Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa232608
Clone ID Related Accession (Same CDS sequence)	XM_024986654.1
Accession Version	XM_024986654.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3858bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 2 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGGCTGGCGG 
GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG 
GGTTACCGAA GGGTGCGCTC CGGCAGTGGA AGCATAGATG ATGACAGGGA TAGCTTGCCT 
GAATGGTGCT TGGAGGACGC TGAAGAGGAA ATGGGCACAT TTGACTCATC TGGAGCGTTC 
CTCTCTCTGA AGAAAGTACA GAAAGAGCCT ATTCCAGAAG AGCAGGAGAT GGACTTCCGA 
CCTGTAGAAG AAGGGGAGGA GTGCTCCGAC TCTGAGGGCA GCCATAATGA AGAAGCCAAA 
GAACCCGATA AGACAAATAA GAAGGAGGCA GAGAAGACAG ACAGAACAGG AGTTGAAGCT 
AGTGAGGAAA CAACCCAGAC CTCCTCTTCA TCTGCTAGAC CAGGTTCTCC TTCAGACCAA 
CCTCAGGAAG CACCACAGTT TGAGAGGAAA GAGGAATCCA AAACTGAGCA AATGGAAAAA 
GCTGAAGAAG AGAGTCGGAC AGAAAATAGT CTATCAACCA AAGTATCCAG CAGGGGGGAT 
GAAGTGCTTC CTGCTGTCCA GCAGCCCCTA TCTCAGATCC CTTCAGATAC AGCCTCTCCG 
CTCCTCATCC TCCCGCCTCC TGTCCCCAGC CCTAGTCCTG CCCTCCGGCC AGTGGAGACG 
CCAGTCGTCG GTGCTCCTGG AATGGGCAGT GTTCCCACAG AGCCAGATGA CGAAGAGGGT 
CTCAAACACT TGGAGCAGCA AGCTGAGAAA ATGGTGGCTT ATCTCCAAGA CAGTGCACTA 
GATGATGAGA GACTGGCATC AAAACTGCAA GAACACAGAG CGAAGGGAGT CTCAATTCCA 
CTGATGCATG AAGCAATGCA GAAGTGGTAT TACAAAGATC CTCAGGGAGA AATTCAAGGT 
CCCTTCAATA ATCAGGAGAT GGCGGAATGG TTTCAGGCGG GCTATTTCAC TATGTCTTTG 
TTGGTAAAGA GAGCATGTGA TGAAAGCTTC CAGCCTCTTG GTGATATCAT GAAAATGTGG 
GGAAGGGTTC CTTTCTCTCC CGGACCAGCT CCCCCTCCGC ATATGGGAGA GCTGGACCAG 
GAACGACTGA CCAGGCAGCA GGAACTCACA GCCTTGTACC AGATGCAGCA CCTTCAGTAT 
CAGCAATTCC TAATACAACA ACAGTATGCG CAGGTTCTGG CCCAGCAGCA GAAAGCAGCG 
CTGTCTTCCC AGCAGCAGCA GCAGTTGGCG CTTCTTCTCC AGCAGTTCCA GGCGCTGAAG 
ATGAGAATAT CTGATCAGAA CATCATTCCC TCAGTAACTA GGTCTGTGTC AGTGCCAGAT 
ACTGGTTCTA TCTGGGAACT CCAGCCAGCA CCCTCACAGC CTACAGTTTG GGAAGGCGGT 
AGCGTATGGG ATCTTCCCCT TGACCCCGCG ACTCCAGCAC CTGCCCTTGA ACAGCTTCAG 
CAGATGGAGA AGGCCAAAGC TGCGAAGCTG GAGCAGGAGA GGAGAGAGGC AGAAATGAGG 
GCGAAACGGG AAGAGGAGGA GCGAAAGAGG CAAGAAGAAC TCCGGAGACA ACAGGAGGAA 
ATTCTTCGGA GACAGCAGGA GGAAGAAAGG AAAAGGCGGG AGGAAGAAGA GCTTGCCCGA 
AGGAAACAGG AAGAAGCTCT GCGACGCCAG AGGGAACAAG AAATTGCTTT AAGGCGACAG 
CGAGAAGAGG AAGAGAGACA GCAGCAGGAA GAAGCTCTTA GGAGACTGGA GGAGAGGAGG 
CGAGAAGAGG AGGAGAGGCG GAAGCAAGAG GAATTGTTAC GCAAGCAGGA AGAGGAGGCT 
GCGAAATGGG CCCGGGAAGA AGAGGAAGCC CAGCGTCGGT TAGAGGAGAA CCGGCTACGG 
ATGGAAGAGG AGGCAGCCAG GCTGCGGCAC GAGGAGGAGG AGCGGAAGCG GAAAGAGCTG 
GAGCTCCAGC GGCAGAAGGA GTTGCTGCGC CAGAGGCAGC AGCAGCAGGA GGCTCTCCGG 
AGGCTGCAGC AGCAGCAGCA GCAGCAGCAG CTGGCTCAGA TGAAGCTTCC TTCTTCTTCA 
ACGTGGGGCC AGCAGTCCAA TACGACAGCA TGTCAGTCCC AGGCAACGCT ATCATTGGCT 
GAAATCCAAA AACTAGAAGA GGAACGAGAA CGGCAGCTTC GAGAGGAGCA GAGGCGCCAG 
CAGAGGGAAT TGATGAAAGC TCTCCAGCAG CAGCAGCAGC AGCAACAGCA GAAACTCTCA 
GGCTGGGGGA ACGTCAGCAA ACCTGCAGGC ACCGCAAAGT CTCTTCTGGA GATCCAACAG 
GAAGAAGCCA GGCAGATGCA GAAGCAGCAG CAACAGCAGC AGCAACACCA GCAGCCAAAC 
AGAGCCCGGA ATAATACGCA TTCCAACCTG CACACCAGCA TTGGGAATTC TGTTTGGGGC 
TCTATAAATA CTGGTCCCCC TAACCAGTGG GCCTCTGACC TAGTCAGTAG TATCTGGAGC 
AGTGCTGACA CTAAAAACTC CAACATGGGA TTCTGGGATG ATGCAGTGAA AGAGGTGGGA 
CCTAGGAACT CAACAAACAA AAATAAAAAC GCCAGTCTCA GTAAATCTGT AGGTGTGTCT 
AACCGGCAGA ATAAGAAAGT AGAAGAAGAA GAAAAATTGC TGAAGCTCTT TCAGGGAGTA 
AATAAAGCCC AAGATGGATT TACCCAGTGG TGTGAACAGA TGCTTCATGC CCTTAATACG 
GCAAATAACT TGGATGTGCC CACATTTGTT TCGTTCCTGA AGGAAGTAGA ATCTCCATAT 
GAGGTCCATG ATTACATTAG GGCCTATTTA GGAGATACCT CTGAGGCCAA GGAGTTTGCC 
AAGCAGTTCC TTGAGCGCCG TGCCAAACAG AAAGCCAACC AGCGGCAACA GCAGCAGCAG 
CAACAGCAAC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGGACTCTGT GTGGGGCATG 
AACCACAGTG CACTCCATTC AGTATTTCAG ACCAATCAGA CCAACAACCA ACAATCCAGT 
TTTGAAGCTG TACAAAGTGG CAAGAAGAAG AAAAAGCAGA AGATGGTCCG AGCAGATCCT 
AGTCTATTAG GGTTTTCAGT CAACGCTTCC TCGGAGCGAC TCAATATGGG TGAGATCGAA 
ACTGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842422.1
CDS	276..4133
Translation	MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGRSSSRGRGRGRGECGFYQRSFDEVEGVFVRGGGREMHRSQSWEERGDRRFDKPGRKDVGRPNFEEGGPVSVGRKHEFIRSESGNWRIFREEQNGEDEDGGWRLTGSRRDGERWRPHSPGWREHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEECSDSEGSHNEEAKEPDKTNKKEAEKTDRTGVEASEETTQTSSSSARPGSPSDQPQEAPQFERKEESKTEQMEKAEEESRTENSLSTKVSSRGDEVLPAVQQPLSQIPSDTASPLLILPPPVPSPSPALRPVETPVVGAPGMGSVPTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQALKMRISDQNIIPSVTRSVSVPDTGSIWELQPAPSQPTVWEGGSVWDLPLDPATPAPALEQLQQMEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELELQRQKELLRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPAGTAKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSSADTKNSNMGFWDDAVKEVGPRNSTNKNKNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQRQQQQQQQQQQQQQQQQQQQDSVWGMNHSALHSVFQTNQTNNQQSSFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVDDY

Target ORF information:

RefSeq Version	XM_024986654.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986654.1

ORF Insert Sequence:

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGGCTGGCGG 
GAACACATGG AACGACGGCG AAGGTTTGAG TTTGATTTTC GAGATCGGGA TGATGAACGG 
GGTTACCGAA GGGTGCGCTC CGGCAGTGGA AGCATAGATG ATGACAGGGA TAGCTTGCCT 
GAATGGTGCT TGGAGGACGC TGAAGAGGAA ATGGGCACAT TTGACTCATC TGGAGCGTTC 
CTCTCTCTGA AGAAAGTACA GAAAGAGCCT ATTCCAGAAG AGCAGGAGAT GGACTTCCGA 
CCTGTAGAAG AAGGGGAGGA GTGCTCCGAC TCTGAGGGCA GCCATAATGA AGAAGCCAAA 
GAACCCGATA AGACAAATAA GAAGGAGGCA GAGAAGACAG ACAGAACAGG AGTTGAAGCT 
AGTGAGGAAA CAACCCAGAC CTCCTCTTCA TCTGCTAGAC CAGGTTCTCC TTCAGACCAA 
CCTCAGGAAG CACCACAGTT TGAGAGGAAA GAGGAATCCA AAACTGAGCA AATGGAAAAA 
GCTGAAGAAG AGAGTCGGAC AGAAAATAGT CTATCAACCA AAGTATCCAG CAGGGGGGAT 
GAAGTGCTTC CTGCTGTCCA GCAGCCCCTA TCTCAGATCC CTTCAGATAC AGCCTCTCCG 
CTCCTCATCC TCCCGCCTCC TGTCCCCAGC CCTAGTCCTG CCCTCCGGCC AGTGGAGACG 
CCAGTCGTCG GTGCTCCTGG AATGGGCAGT GTTCCCACAG AGCCAGATGA CGAAGAGGGT 
CTCAAACACT TGGAGCAGCA AGCTGAGAAA ATGGTGGCTT ATCTCCAAGA CAGTGCACTA 
GATGATGAGA GACTGGCATC AAAACTGCAA GAACACAGAG CGAAGGGAGT CTCAATTCCA 
CTGATGCATG AAGCAATGCA GAAGTGGTAT TACAAAGATC CTCAGGGAGA AATTCAAGGT 
CCCTTCAATA ATCAGGAGAT GGCGGAATGG TTTCAGGCGG GCTATTTCAC TATGTCTTTG 
TTGGTAAAGA GAGCATGTGA TGAAAGCTTC CAGCCTCTTG GTGATATCAT GAAAATGTGG 
GGAAGGGTTC CTTTCTCTCC CGGACCAGCT CCCCCTCCGC ATATGGGAGA GCTGGACCAG 
GAACGACTGA CCAGGCAGCA GGAACTCACA GCCTTGTACC AGATGCAGCA CCTTCAGTAT 
CAGCAATTCC TAATACAACA ACAGTATGCG CAGGTTCTGG CCCAGCAGCA GAAAGCAGCG 
CTGTCTTCCC AGCAGCAGCA GCAGTTGGCG CTTCTTCTCC AGCAGTTCCA GGCGCTGAAG 
ATGAGAATAT CTGATCAGAA CATCATTCCC TCAGTAACTA GGTCTGTGTC AGTGCCAGAT 
ACTGGTTCTA TCTGGGAACT CCAGCCAGCA CCCTCACAGC CTACAGTTTG GGAAGGCGGT 
AGCGTATGGG ATCTTCCCCT TGACCCCGCG ACTCCAGCAC CTGCCCTTGA ACAGCTTCAG 
CAGATGGAGA AGGCCAAAGC TGCGAAGCTG GAGCAGGAGA GGAGAGAGGC AGAAATGAGG 
GCGAAACGGG AAGAGGAGGA GCGAAAGAGG CAAGAAGAAC TCCGGAGACA ACAGGAGGAA 
ATTCTTCGGA GACAGCAGGA GGAAGAAAGG AAAAGGCGGG AGGAAGAAGA GCTTGCCCGA 
AGGAAACAGG AAGAAGCTCT GCGACGCCAG AGGGAACAAG AAATTGCTTT AAGGCGACAG 
CGAGAAGAGG AAGAGAGACA GCAGCAGGAA GAAGCTCTTA GGAGACTGGA GGAGAGGAGG 
CGAGAAGAGG AGGAGAGGCG GAAGCAAGAG GAATTGTTAC GCAAGCAGGA AGAGGAGGCT 
GCGAAATGGG CCCGGGAAGA AGAGGAAGCC CAGCGTCGGT TAGAGGAGAA CCGGCTACGG 
ATGGAAGAGG AGGCAGCCAG GCTGCGGCAC GAGGAGGAGG AGCGGAAGCG GAAAGAGCTG 
GAGCTCCAGC GGCAGAAGGA GTTGCTGCGC CAGAGGCAGC AGCAGCAGGA GGCTCTCCGG 
AGGCTGCAGC AGCAGCAGCA GCAGCAGCAG CTGGCTCAGA TGAAGCTTCC TTCTTCTTCA 
ACGTGGGGCC AGCAGTCCAA TACGACAGCA TGTCAGTCCC AGGCAACGCT ATCATTGGCT 
GAAATCCAAA AACTAGAAGA GGAACGAGAA CGGCAGCTTC GAGAGGAGCA GAGGCGCCAG 
CAGAGGGAAT TGATGAAAGC TCTCCAGCAG CAGCAGCAGC AGCAACAGCA GAAACTCTCA 
GGCTGGGGGA ACGTCAGCAA ACCTGCAGGC ACCGCAAAGT CTCTTCTGGA GATCCAACAG 
GAAGAAGCCA GGCAGATGCA GAAGCAGCAG CAACAGCAGC AGCAACACCA GCAGCCAAAC 
AGAGCCCGGA ATAATACGCA TTCCAACCTG CACACCAGCA TTGGGAATTC TGTTTGGGGC 
TCTATAAATA CTGGTCCCCC TAACCAGTGG GCCTCTGACC TAGTCAGTAG TATCTGGAGC 
AGTGCTGACA CTAAAAACTC CAACATGGGA TTCTGGGATG ATGCAGTGAA AGAGGTGGGA 
CCTAGGAACT CAACAAACAA AAATAAAAAC GCCAGTCTCA GTAAATCTGT AGGTGTGTCT 
AACCGGCAGA ATAAGAAAGT AGAAGAAGAA GAAAAATTGC TGAAGCTCTT TCAGGGAGTA 
AATAAAGCCC AAGATGGATT TACCCAGTGG TGTGAACAGA TGCTTCATGC CCTTAATACG 
GCAAATAACT TGGATGTGCC CACATTTGTT TCGTTCCTGA AGGAAGTAGA ATCTCCATAT 
GAGGTCCATG ATTACATTAG GGCCTATTTA GGAGATACCT CTGAGGCCAA GGAGTTTGCC 
AAGCAGTTCC TTGAGCGCCG TGCCAAACAG AAAGCCAACC AGCGGCAACA GCAGCAGCAG 
CAACAGCAAC AGCAGCAGCA GCAGCAGCAG CAGCAGCAGC AGGACTCTGT GTGGGGCATG 
AACCACAGTG CACTCCATTC AGTATTTCAG ACCAATCAGA CCAACAACCA ACAATCCAGT 
TTTGAAGCTG TACAAAGTGG CAAGAAGAAG AAAAAGCAGA AGATGGTCCG AGCAGATCCT 
AGTCTATTAG GGTTTTCAGT CAACGCTTCC TCGGAGCGAC TCAATATGGG TGAGATCGAA 
ACTGTGGATG ACTACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( XM_024986656.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024842424.1 Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA.

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CloneID	OBa232609
Clone ID Related Accession (Same CDS sequence)	XM_024986656.1
Accession Version	XM_024986656.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 2 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037330.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG GAGACCAAAT TTTGAGGAAG GCGGACCAGT GTCAGTGGGA 
AGAAAGCATG AATTTATACG ATCAGAGAGT GGAAATTGGC GTATTTTCAG AGAGGAACAG 
AATGGAGAAG ATGAAGATGG AGGCTGGCGA CTAACTGGAT CGAGAAGGGA TGGAGAAAGG 
TGGCGGCCTC ACAGCCCTGA TGGCCCTCGT TCTGCAGGCT GGCGGGAACA CATGGAACGA 
CGGCGAAGGT TTGAGTTTGA TTTTCGAGAT CGGGATGATG AACGGGGTTA CCGAAGGGTG 
CGCTCCGGCA GTGGAAGCAT AGATGATGAC AGGGATAGCT TGCCTGAATG GTGCTTGGAG 
GACGCTGAAG AGGAAATGGG CACATTTGAC TCATCTGGAG CGTTCCTCTC TCTGAAGAAA 
GTACAGAAAG AGCCTATTCC AGAAGAGCAG GAGATGGACT TCCGACCTGT AGAAGAAGGG 
GAGGAGTGCT CCGACTCTGA GGGCAGCCAT AATGAAGAAG CCAAAGAACC CGATAAGACA 
AATAAGAAGG AGGCAGAGAA GACAGACAGA ACAGGAGTTG AAGCTAGTGA GGAAACAACC 
CAGACCTCCT CTTCATCTGC TAGACCAGGT TCTCCTTCAG ACCAACCTCA GGAAGCACCA 
CAGTTTGAGA GGAAAGAGGA ATCCAAAACT GAGCAAATGG AAAAAGCTGA AGAAGAGAGT 
CGGACAGAAA ATAGTCTATC AACCAAAGTA TCCAGCAGGG GGGATGAAGT GCTTCCTGCT 
GTCCAGCAGC CCCTATCTCA GATCCCTTCA GATACAGCCT CTCCGCTCCT CATCCTCCCG 
CCTCCTGTCC CCAGCCCTAG TCCTGCCCTC CGGCCAGTGG AGACGCCAGT CGTCGGTGCT 
CCTGGAATGG GCAGTGTTCC CACAGAGCCA GATGACGAAG AGGGTCTCAA ACACTTGGAG 
CAGCAAGCTG AGAAAATGGT GGCTTATCTC CAAGACAGTG CACTAGATGA TGAGAGACTG 
GCATCAAAAC TGCAAGAACA CAGAGCGAAG GGAGTCTCAA TTCCACTGAT GCATGAAGCA 
ATGCAGAAGT GGTATTACAA AGATCCTCAG GGAGAAATTC AAGGTCCCTT CAATAATCAG 
GAGATGGCGG AATGGTTTCA GGCGGGCTAT TTCACTATGT CTTTGTTGGT AAAGAGAGCA 
TGTGATGAAA GCTTCCAGCC TCTTGGTGAT ATCATGAAAA TGTGGGGAAG GGTTCCTTTC 
TCTCCCGGAC CAGCTCCCCC TCCGCATATG GGAGAGCTGG ACCAGGAACG ACTGACCAGG 
CAGCAGGAAC TCACAGCCTT GTACCAGATG CAGCACCTTC AGTATCAGCA ATTCCTAATA 
CAACAACAGT ATGCGCAGGT TCTGGCCCAG CAGCAGAAAG CAGCGCTGTC TTCCCAGCAG 
CAGCAGCAGT TGGCGCTTCT TCTCCAGCAG TTCCAGGCGC TGAAGATGAG AATATCTGAT 
CAGAACATCA TTCCCTCAGT AACTAGGTCT GTGTCAGTGC CAGATACTGG TTCTATCTGG 
GAACTCCAGC CAGCACCCTC ACAGCCTACA GTTTGGGAAG GCGGTAGCGT ATGGGATCTT 
CCCCTTGACC CCGCGACTCC AGCACCTGCC CTTGAACAGC TTCAGCAGAT GGAGAAGGCC 
AAAGCTGCGA AGCTGGAGCA GGAGAGGAGA GAGGCAGAAA TGAGGGCGAA ACGGGAAGAG 
GAGGAGCGAA AGAGGCAAGA AGAACTCCGG AGACAACAGG AGGAAATTCT TCGGAGACAG 
CAGGAGGAAG AAAGGAAAAG GCGGGAGGAA GAAGAGCTTG CCCGAAGGAA ACAGGAAGAA 
GCTCTGCGAC GCCAGAGGGA ACAAGAAATT GCTTTAAGGC GACAGCGAGA AGAGGAAGAG 
AGACAGCAGC AGGAAGAAGC TCTTAGGAGA CTGGAGGAGA GGAGGCGAGA AGAGGAGGAG 
AGGCGGAAGC AAGAGGAATT GTTACGCAAG CAGGAAGAGG AGGCTGCGAA ATGGGCCCGG 
GAAGAAGAGG AAGCCCAGCG TCGGTTAGAG GAGAACCGGC TACGGATGGA AGAGGAGGCA 
GCCAGGCTGC GGCACGAGGA GGAGGAGCGG AAGCGGAAAG AGCTGGAGCT CCAGCGGCAG 
AAGGAGTTGC TGCGCCAGAG GCAGCAGCAG CAGGAGGCTC TCCGGAGGCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCTGGC TCAGATGAAG CTTCCTTCTT CTTCAACGTG GGGCCAGCAG 
TCCAATACGA CAGCATGTCA GTCCCAGGCA ACGCTATCAT TGGCTGAAAT CCAAAAACTA 
GAAGAGGAAC GAGAACGGCA GCTTCGAGAG GAGCAGAGGC GCCAGCAGAG GGAATTGATG 
AAAGCTCTCC AGCAGCAGCA GCAGCAGCAA CAGCAGAAAC TCTCAGGCTG GGGGAACGTC 
AGCAAACCTG CAGGCACCGC AAAGTCTCTT CTGGAGATCC AACAGGAAGA AGCCAGGCAG 
ATGCAGAAGC AGCAGCAACA GCAGCAGCAA CACCAGCAGC CAAACAGAGC CCGGAATAAT 
ACGCATTCCA ACCTGCACAC CAGCATTGGG AATTCTGTTT GGGGCTCTAT AAATACTGGT 
CCCCCTAACC AGTGGGCCTC TGACCTAGTC AGTAGTATCT GGAGCAGTGC TGACACTAAA 
AACTCCAACA TGGGATTCTG GGATGATGCA GTGAAAGAGG TGGGACCTAG GAACTCAACA 
AACAAAAATA AAAACGCCAG TCTCAGTAAA TCTGTAGGTG TGTCTAACCG GCAGAATAAG 
AAAGTAGAAG AAGAAGAAAA ATTGCTGAAG CTCTTTCAGG GAGTAAATAA AGCCCAAGAT 
GGATTTACCC AGTGGTGTGA ACAGATGCTT CATGCCCTTA ATACGGCAAA TAACTTGGAT 
GTGCCCACAT TTGTTTCGTT CCTGAAGGAA GTAGAATCTC CATATGAGGT CCATGATTAC 
ATTAGGGCCT ATTTAGGAGA TACCTCTGAG GCCAAGGAGT TTGCCAAGCA GTTCCTTGAG 
CGCCGTGCCA AACAGAAAGC CAACCAGCGG CAACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC TCTGTGTGGG GCATGAACCA CAGTGCACTC 
CATTCAGTAT TTCAGACCAA TCAGACCAAC AACCAACAAT CCAGTTTTGA AGCTGTACAA 
AGTGGCAAGA AGAAGAAAAA GCAGAAGATG GTCCGAGCAG ATCCTAGTCT ATTAGGGTTT 
TCAGTCAACG CTTCCTCGGA GCGACTCAAT ATGGGTGAGA TCGAAACTGT GGATGACTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024842424.1
CDS	276..3998
Translation	MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGRSSSRGRGRPNFEEGGPVSVGRKHEFIRSESGNWRIFREEQNGEDEDGGWRLTGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEECSDSEGSHNEEAKEPDKTNKKEAEKTDRTGVEASEETTQTSSSSARPGSPSDQPQEAPQFERKEESKTEQMEKAEEESRTENSLSTKVSSRGDEVLPAVQQPLSQIPSDTASPLLILPPPVPSPSPALRPVETPVVGAPGMGSVPTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQALKMRISDQNIIPSVTRSVSVPDTGSIWELQPAPSQPTVWEGGSVWDLPLDPATPAPALEQLQQMEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRRQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELELQRQKELLRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPAGTAKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSSADTKNSNMGFWDDAVKEVGPRNSTNKNKNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQRQQQQQQQQQQQQQQQQQQQDSVWGMNHSALHSVFQTNQTNNQQSSFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVDDY

Target ORF information:

RefSeq Version	XM_024986656.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024986656.1

ORF Insert Sequence:

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG GAGACCAAAT TTTGAGGAAG GCGGACCAGT GTCAGTGGGA 
AGAAAGCATG AATTTATACG ATCAGAGAGT GGAAATTGGC GTATTTTCAG AGAGGAACAG 
AATGGAGAAG ATGAAGATGG AGGCTGGCGA CTAACTGGAT CGAGAAGGGA TGGAGAAAGG 
TGGCGGCCTC ACAGCCCTGA TGGCCCTCGT TCTGCAGGCT GGCGGGAACA CATGGAACGA 
CGGCGAAGGT TTGAGTTTGA TTTTCGAGAT CGGGATGATG AACGGGGTTA CCGAAGGGTG 
CGCTCCGGCA GTGGAAGCAT AGATGATGAC AGGGATAGCT TGCCTGAATG GTGCTTGGAG 
GACGCTGAAG AGGAAATGGG CACATTTGAC TCATCTGGAG CGTTCCTCTC TCTGAAGAAA 
GTACAGAAAG AGCCTATTCC AGAAGAGCAG GAGATGGACT TCCGACCTGT AGAAGAAGGG 
GAGGAGTGCT CCGACTCTGA GGGCAGCCAT AATGAAGAAG CCAAAGAACC CGATAAGACA 
AATAAGAAGG AGGCAGAGAA GACAGACAGA ACAGGAGTTG AAGCTAGTGA GGAAACAACC 
CAGACCTCCT CTTCATCTGC TAGACCAGGT TCTCCTTCAG ACCAACCTCA GGAAGCACCA 
CAGTTTGAGA GGAAAGAGGA ATCCAAAACT GAGCAAATGG AAAAAGCTGA AGAAGAGAGT 
CGGACAGAAA ATAGTCTATC AACCAAAGTA TCCAGCAGGG GGGATGAAGT GCTTCCTGCT 
GTCCAGCAGC CCCTATCTCA GATCCCTTCA GATACAGCCT CTCCGCTCCT CATCCTCCCG 
CCTCCTGTCC CCAGCCCTAG TCCTGCCCTC CGGCCAGTGG AGACGCCAGT CGTCGGTGCT 
CCTGGAATGG GCAGTGTTCC CACAGAGCCA GATGACGAAG AGGGTCTCAA ACACTTGGAG 
CAGCAAGCTG AGAAAATGGT GGCTTATCTC CAAGACAGTG CACTAGATGA TGAGAGACTG 
GCATCAAAAC TGCAAGAACA CAGAGCGAAG GGAGTCTCAA TTCCACTGAT GCATGAAGCA 
ATGCAGAAGT GGTATTACAA AGATCCTCAG GGAGAAATTC AAGGTCCCTT CAATAATCAG 
GAGATGGCGG AATGGTTTCA GGCGGGCTAT TTCACTATGT CTTTGTTGGT AAAGAGAGCA 
TGTGATGAAA GCTTCCAGCC TCTTGGTGAT ATCATGAAAA TGTGGGGAAG GGTTCCTTTC 
TCTCCCGGAC CAGCTCCCCC TCCGCATATG GGAGAGCTGG ACCAGGAACG ACTGACCAGG 
CAGCAGGAAC TCACAGCCTT GTACCAGATG CAGCACCTTC AGTATCAGCA ATTCCTAATA 
CAACAACAGT ATGCGCAGGT TCTGGCCCAG CAGCAGAAAG CAGCGCTGTC TTCCCAGCAG 
CAGCAGCAGT TGGCGCTTCT TCTCCAGCAG TTCCAGGCGC TGAAGATGAG AATATCTGAT 
CAGAACATCA TTCCCTCAGT AACTAGGTCT GTGTCAGTGC CAGATACTGG TTCTATCTGG 
GAACTCCAGC CAGCACCCTC ACAGCCTACA GTTTGGGAAG GCGGTAGCGT ATGGGATCTT 
CCCCTTGACC CCGCGACTCC AGCACCTGCC CTTGAACAGC TTCAGCAGAT GGAGAAGGCC 
AAAGCTGCGA AGCTGGAGCA GGAGAGGAGA GAGGCAGAAA TGAGGGCGAA ACGGGAAGAG 
GAGGAGCGAA AGAGGCAAGA AGAACTCCGG AGACAACAGG AGGAAATTCT TCGGAGACAG 
CAGGAGGAAG AAAGGAAAAG GCGGGAGGAA GAAGAGCTTG CCCGAAGGAA ACAGGAAGAA 
GCTCTGCGAC GCCAGAGGGA ACAAGAAATT GCTTTAAGGC GACAGCGAGA AGAGGAAGAG 
AGACAGCAGC AGGAAGAAGC TCTTAGGAGA CTGGAGGAGA GGAGGCGAGA AGAGGAGGAG 
AGGCGGAAGC AAGAGGAATT GTTACGCAAG CAGGAAGAGG AGGCTGCGAA ATGGGCCCGG 
GAAGAAGAGG AAGCCCAGCG TCGGTTAGAG GAGAACCGGC TACGGATGGA AGAGGAGGCA 
GCCAGGCTGC GGCACGAGGA GGAGGAGCGG AAGCGGAAAG AGCTGGAGCT CCAGCGGCAG 
AAGGAGTTGC TGCGCCAGAG GCAGCAGCAG CAGGAGGCTC TCCGGAGGCT GCAGCAGCAG 
CAGCAGCAGC AGCAGCTGGC TCAGATGAAG CTTCCTTCTT CTTCAACGTG GGGCCAGCAG 
TCCAATACGA CAGCATGTCA GTCCCAGGCA ACGCTATCAT TGGCTGAAAT CCAAAAACTA 
GAAGAGGAAC GAGAACGGCA GCTTCGAGAG GAGCAGAGGC GCCAGCAGAG GGAATTGATG 
AAAGCTCTCC AGCAGCAGCA GCAGCAGCAA CAGCAGAAAC TCTCAGGCTG GGGGAACGTC 
AGCAAACCTG CAGGCACCGC AAAGTCTCTT CTGGAGATCC AACAGGAAGA AGCCAGGCAG 
ATGCAGAAGC AGCAGCAACA GCAGCAGCAA CACCAGCAGC CAAACAGAGC CCGGAATAAT 
ACGCATTCCA ACCTGCACAC CAGCATTGGG AATTCTGTTT GGGGCTCTAT AAATACTGGT 
CCCCCTAACC AGTGGGCCTC TGACCTAGTC AGTAGTATCT GGAGCAGTGC TGACACTAAA 
AACTCCAACA TGGGATTCTG GGATGATGCA GTGAAAGAGG TGGGACCTAG GAACTCAACA 
AACAAAAATA AAAACGCCAG TCTCAGTAAA TCTGTAGGTG TGTCTAACCG GCAGAATAAG 
AAAGTAGAAG AAGAAGAAAA ATTGCTGAAG CTCTTTCAGG GAGTAAATAA AGCCCAAGAT 
GGATTTACCC AGTGGTGTGA ACAGATGCTT CATGCCCTTA ATACGGCAAA TAACTTGGAT 
GTGCCCACAT TTGTTTCGTT CCTGAAGGAA GTAGAATCTC CATATGAGGT CCATGATTAC 
ATTAGGGCCT ATTTAGGAGA TACCTCTGAG GCCAAGGAGT TTGCCAAGCA GTTCCTTGAG 
CGCCGTGCCA AACAGAAAGC CAACCAGCGG CAACAGCAGC AGCAGCAACA GCAACAGCAG 
CAGCAGCAGC AGCAGCAGCA GCAGCAGGAC TCTGTGTGGG GCATGAACCA CAGTGCACTC 
CATTCAGTAT TTCAGACCAA TCAGACCAAC AACCAACAAT CCAGTTTTGA AGCTGTACAA 
AGTGGCAAGA AGAAGAAAAA GCAGAAGATG GTCCGAGCAG ATCCTAGTCT ATTAGGGTTT 
TCAGTCAACG CTTCCTCGGA GCGACTCAAT ATGGGTGAGA TCGAAACTGT GGATGACTAC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GIGYF2 ( NM_001101877.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001095347.1 Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), mRNA.

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CloneID	OBa38871
Clone ID Related Accession (Same CDS sequence)	NM_001101877.1
Accession Version	NM_001101877.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-13
Organism	Bos taurus(cattle)
Product	GRB10-interacting GYF protein 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC151282.1. On Aug 21, 2007 this sequence version replaced XM_881454.2. ##Evidence-Data-START## Transcript exon combination :: BC151282.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCAACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
GACTCTGTGT GGGGCATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAGACC 
AACAACCAAC AATCCAGTTT TGAAGCTGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG 
ATGGTCCGAG CAGATCCTAG TCTATTAGGG TTTTCAGTCA ACGCTTCCTC GGAGCGACTC 
AATATGGGTG AGATCGAAAC TGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001095347.1
CDS	228..4103
Translation	MAAETQTLNFGPEWLRALSSGGSITSPPLSPALPKYKLADYRYGREEMLALFLKDNKIPSDLLDKEFLPILQEEPLLPLALVPFTEEEQRNFSMSVNSAAVLRLTGRGGGGTVVGAPRGRSSSRGRGRGRGECGFYQRSFDEVEGVFVRGGGREMHRSQSWEERGDRRFDKPGRKDVGRPNFEEGGPVSVGRKHEFIRSESGNWRIFREEQNGEDEDGGWRLTGSRRDGERWRPHSPDGPRSAGWREHMERRRRFEFDFRDRDDERGYRRVRSGSGSIDDDRDSLPEWCLEDAEEEMGTFDSSGAFLSLKKVQKEPIPEEQEMDFRPVEEGEECSDSEGSHNEEAKEPDKTNKKEAEKTDRTGVEASEETTQTSSSSARPGSPSDQPQEAPQFERKEESKTEQMEKAEEESRTENSLSTKVSSRGDEVLPAVQQPLSQIPSDTASPLLILPPPVPSPSPALRPVETPVVGAPGMGSVPTEPDDEEGLKHLEQQAEKMVAYLQDSALDDERLASKLQEHRAKGVSIPLMHEAMQKWYYKDPQGEIQGPFNNQEMAEWFQAGYFTMSLLVKRACDESFQPLGDIMKMWGRVPFSPGPAPPPHMGELDQERLTRQQELTALYQMQHLQYQQFLIQQQYAQVLAQQQKAALSSQQQQQLALLLQQFQALKMRISDQNIIPSVTRSVSVPDTGSIWELQPAPSQPTVWEGGSVWDLPLDPATPAPALEQLQQMEKAKAAKLEQERREAEMRAKREEEERKRQEELRRQQEEILRRQQEEERKRREEEELARRKQEEALRRQREQEIALRQQREEEERQQQEEALRRLEERRREEEERRKQEELLRKQEEEAAKWAREEEEAQRRLEENRLRMEEEAARLRHEEEERKRKELELQRQKELLRQRQQQQEALRRLQQQQQQQQLAQMKLPSSSTWGQQSNTTACQSQATLSLAEIQKLEEERERQLREEQRRQQRELMKALQQQQQQQQQKLSGWGNVSKPAGTAKSLLEIQQEEARQMQKQQQQQQQHQQPNRARNNTHSNLHTSIGNSVWGSINTGPPNQWASDLVSSIWSSADTKNSNMGFWDDAVKEVGPRNSTNKNKNASLSKSVGVSNRQNKKVEEEEKLLKLFQGVNKAQDGFTQWCEQMLHALNTANNLDVPTFVSFLKEVESPYEVHDYIRAYLGDTSEAKEFAKQFLERRAKQKANQRQQQQQQQQQQQQQQQQQQQDSVWGMNHSALHSVFQTNQTNNQQSSFEAVQSGKKKKKQKMVRADPSLLGFSVNASSERLNMGEIETVDDY

Target ORF information:

RefSeq Version	NM_001101877.1
Organism	Bos taurus(cattle)
Definition	Bos taurus GRB10 interacting GYF protein 2 (GIGYF2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001101877.1

ORF Insert Sequence:

ATGGCAGCGG AAACGCAGAC ACTGAACTTT GGGCCTGAAT GGCTCCGAGC TCTGTCCAGC 
GGTGGGAGTA TTACATCCCC CCCTCTTTCG CCAGCATTGC CGAAGTATAA GTTAGCAGAC 
TATCGTTACG GGCGAGAAGA AATGTTAGCA CTTTTCCTTA AAGACAATAA GATCCCTTCA 
GACCTTCTGG ATAAAGAATT TCTGCCTATC CTACAGGAGG AGCCCCTGCT GCCACTGGCT 
CTTGTGCCCT TCACGGAAGA AGAACAGAGA AACTTTTCCA TGTCCGTAAA TAGTGCTGCT 
GTCCTGCGAT TGACAGGACG AGGAGGAGGA GGAACGGTGG TGGGTGCTCC AAGAGGTCGA 
AGTTCTTCAC GAGGGCGAGG CAGAGGCAGA GGTGAATGTG GTTTCTACCA AAGAAGCTTT 
GATGAAGTAG AGGGGGTGTT TGTCCGAGGA GGCGGCAGGG AAATGCACAG GTCACAGAGC 
TGGGAGGAAA GGGGTGACAG ACGTTTTGAC AAACCAGGAC GAAAAGATGT AGGGAGACCA 
AATTTTGAGG AAGGCGGACC AGTGTCAGTG GGAAGAAAGC ATGAATTTAT ACGATCAGAG 
AGTGGAAATT GGCGTATTTT CAGAGAGGAA CAGAATGGAG AAGATGAAGA TGGAGGCTGG 
CGACTAACTG GATCGAGAAG GGATGGAGAA AGGTGGCGGC CTCACAGCCC TGATGGCCCT 
CGTTCTGCAG GCTGGCGGGA ACACATGGAA CGACGGCGAA GGTTTGAGTT TGATTTTCGA 
GATCGGGATG ATGAACGGGG TTACCGAAGG GTGCGCTCCG GCAGTGGAAG CATAGATGAT 
GACAGGGATA GCTTGCCTGA ATGGTGCTTG GAGGACGCTG AAGAGGAAAT GGGCACATTT 
GACTCATCTG GAGCGTTCCT CTCTCTGAAG AAAGTACAGA AAGAGCCTAT TCCAGAAGAG 
CAGGAGATGG ACTTCCGACC TGTAGAAGAA GGGGAGGAGT GCTCCGACTC TGAGGGCAGC 
CATAATGAAG AAGCCAAAGA ACCCGATAAG ACAAATAAGA AGGAGGCAGA GAAGACAGAC 
AGAACAGGAG TTGAAGCTAG TGAGGAAACA ACCCAGACCT CCTCTTCATC TGCTAGACCA 
GGTTCTCCTT CAGACCAACC TCAGGAAGCA CCACAGTTTG AGAGGAAAGA GGAATCCAAA 
ACTGAGCAAA TGGAAAAAGC TGAAGAAGAG AGTCGGACAG AAAATAGTCT ATCAACCAAA 
GTATCCAGCA GGGGGGATGA AGTGCTTCCT GCTGTCCAGC AGCCCCTATC TCAGATCCCT 
TCAGATACAG CCTCTCCGCT CCTCATCCTC CCGCCTCCTG TCCCCAGCCC TAGTCCTGCC 
CTCCGGCCAG TGGAGACGCC AGTCGTCGGT GCTCCTGGAA TGGGCAGTGT TCCCACAGAG 
CCAGATGACG AAGAGGGTCT CAAACACTTG GAGCAGCAAG CTGAGAAAAT GGTGGCTTAT 
CTCCAAGACA GTGCACTAGA TGATGAGAGA CTGGCATCAA AACTGCAAGA ACACAGAGCG 
AAGGGAGTCT CAATTCCACT GATGCATGAA GCAATGCAGA AGTGGTATTA CAAAGATCCT 
CAGGGAGAAA TTCAAGGTCC CTTCAATAAT CAGGAGATGG CGGAATGGTT TCAGGCGGGC 
TATTTCACTA TGTCTTTGTT GGTAAAGAGA GCATGTGATG AAAGCTTCCA GCCTCTTGGT 
GATATCATGA AAATGTGGGG AAGGGTTCCT TTCTCTCCCG GACCAGCTCC CCCTCCGCAT 
ATGGGAGAGC TGGACCAGGA ACGACTGACC AGGCAGCAGG AACTCACAGC CTTGTACCAG 
ATGCAGCACC TTCAGTATCA GCAATTCCTA ATACAACAAC AGTATGCGCA GGTTCTGGCC 
CAGCAGCAGA AAGCAGCGCT GTCTTCCCAG CAGCAGCAGC AGTTGGCGCT TCTTCTCCAG 
CAGTTCCAGG CGCTGAAGAT GAGAATATCT GATCAGAACA TCATTCCCTC AGTAACTAGG 
TCTGTGTCAG TGCCAGATAC TGGTTCTATC TGGGAACTCC AGCCAGCACC CTCACAGCCT 
ACAGTTTGGG AAGGCGGTAG CGTATGGGAT CTTCCCCTTG ACCCCGCGAC TCCAGCACCT 
GCCCTTGAAC AGCTTCAGCA GATGGAGAAG GCCAAAGCTG CGAAGCTGGA GCAGGAGAGG 
AGAGAGGCAG AAATGAGGGC GAAACGGGAA GAGGAGGAGC GAAAGAGGCA AGAAGAACTC 
CGGAGACAAC AGGAGGAAAT TCTTCGGAGA CAGCAGGAGG AAGAAAGGAA AAGGCGGGAG 
GAAGAAGAGC TTGCCCGAAG GAAACAGGAA GAAGCTCTGC GACGCCAGAG GGAACAAGAA 
ATTGCTTTAA GGCAACAGCG AGAAGAGGAA GAGAGACAGC AGCAGGAAGA AGCTCTTAGG 
AGACTGGAGG AGAGGAGGCG AGAAGAGGAG GAGAGGCGGA AGCAAGAGGA ATTGTTACGC 
AAGCAGGAAG AGGAGGCTGC GAAATGGGCC CGGGAAGAAG AGGAAGCCCA GCGTCGGTTA 
GAGGAGAACC GGCTACGGAT GGAAGAGGAG GCAGCCAGGC TGCGGCACGA GGAGGAGGAG 
CGGAAGCGGA AAGAGCTGGA GCTCCAGCGG CAGAAGGAGT TGCTGCGCCA GAGGCAGCAG 
CAGCAGGAGG CTCTCCGGAG GCTGCAGCAG CAGCAGCAGC AGCAGCAGCT GGCTCAGATG 
AAGCTTCCTT CTTCTTCAAC GTGGGGCCAG CAGTCCAATA CGACAGCATG TCAGTCCCAG 
GCAACGCTAT CATTGGCTGA AATCCAAAAA CTAGAAGAGG AACGAGAACG GCAGCTTCGA 
GAGGAGCAGA GGCGCCAGCA GAGGGAATTG ATGAAAGCTC TCCAGCAGCA GCAGCAGCAG 
CAACAGCAGA AACTCTCAGG CTGGGGGAAC GTCAGCAAAC CTGCAGGCAC CGCAAAGTCT 
CTTCTGGAGA TCCAACAGGA AGAAGCCAGG CAGATGCAGA AGCAGCAGCA ACAGCAGCAG 
CAACACCAGC AGCCAAACAG AGCCCGGAAT AATACGCATT CCAACCTGCA CACCAGCATT 
GGGAATTCTG TTTGGGGCTC TATAAATACT GGTCCCCCTA ACCAGTGGGC CTCTGACCTA 
GTCAGTAGTA TCTGGAGCAG TGCTGACACT AAAAACTCCA ACATGGGATT CTGGGATGAT 
GCAGTGAAAG AGGTGGGACC TAGGAACTCA ACAAACAAAA ATAAAAACGC CAGTCTCAGT 
AAATCTGTAG GTGTGTCTAA CCGGCAGAAT AAGAAAGTAG AAGAAGAAGA AAAATTGCTG 
AAGCTCTTTC AGGGAGTAAA TAAAGCCCAA GATGGATTTA CCCAGTGGTG TGAACAGATG 
CTTCATGCCC TTAATACGGC AAATAACTTG GATGTGCCCA CATTTGTTTC GTTCCTGAAG 
GAAGTAGAAT CTCCATATGA GGTCCATGAT TACATTAGGG CCTATTTAGG AGATACCTCT 
GAGGCCAAGG AGTTTGCCAA GCAGTTCCTT GAGCGCCGTG CCAAACAGAA AGCCAACCAG 
CGGCAACAGC AGCAGCAGCA ACAGCAACAG CAGCAGCAGC AGCAGCAGCA GCAGCAGCAG 
GACTCTGTGT GGGGCATGAA CCACAGTGCA CTCCATTCAG TATTTCAGAC CAATCAGACC 
AACAACCAAC AATCCAGTTT TGAAGCTGTA CAAAGTGGCA AGAAGAAGAA AAAGCAGAAG 
ATGGTCCGAG CAGATCCTAG TCTATTAGGG TTTTCAGTCA ACGCTTCCTC GGAGCGACTC 
AATATGGGTG AGATCGAAAC TGTGGATGAC TACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A whole-genome assembly of the domestic cow, Bos taurus.
Genome biology10(4)R42(2009)
Zimin AV,Delcher AL,Florea L,Kelley DR,Schatz MC,Puiu D,Hanrahan F,Pertea G,Van Tassell CP,Sonstegard TS,Mar?ais G,Roberts M,Subramanian P,Yorke JA,Salzberg SL

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GIGYF2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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