micall2b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	micall2b
Entrez Gene ID	556298
Full Name	mical-like 2b
Synonyms	MICAL-2,zgc:55983,zgc:66216
General protein information	Preferred Names mical-like 2b Names protein-methionine sulfoxide oxidase mical2b MICAL-like 2 molecule interacting with CasL protein 2B molecule interacting with CasL-like 2b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017354911.1	XP_017210400.1	protein-methionine sulfoxide oxidase mical2b isoform X1
NM_001312897.1	NP_001299826.1	protein-methionine sulfoxide oxidase mical2b

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

A new locus regulating MICALL2 expression was identified for association with executive inhibition in children with attention deficit hyperactivity disorder.
Yang L, Chang S, Lu Q, Zhang Y, Wu Z, Sun X, Cao Q, Qian Y, Jia T, Xu B, Duan Q, Li Y, Zhang K, Schumann G, Liu D, Wang J, Wang Y, Lu L
Molecular psychiatry(2017 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Novel cardiovascular gene functions revealed via systematic phenotype prediction in zebrafish.
Musso G, Tasan M, Mosimann C, Beaver JE, Plovie E, Carr LA, Chua HN, Dunham J, Zuberi K, Rodriguez H, Morris Q, Zon L, Roth FP, MacRae CA
Development (Cambridge, England)141(1)224-35(2014 Jan)

Identification and expression analysis of mical family genes in zebrafish.
Xue Y, Kuok C, Xiao A, Zhu Z, Lin S, Zhang B
Journal of genetics and genomics = Yi chuan xue bao37(10)685-93(2010 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data established an important basis for further functional studies of mical family genes in zebrafish, and suggest a possible role for mical genes in cardiovascular development.
Title: Identification and expression analysis of mical family genes in zebrafish.

The following micall2b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the micall2b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa05420	NM_001312897.1 Latest version!	Danio rerio mical-like 2b (micall2b), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa53702	XM_017354911.1 Latest version!	Danio rerio mical-like 2b (micall2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

micall2b ( NM_001312897.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001299826.1 Danio rerio mical-like 2b (micall2b), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa05420
Clone ID Related Accession (Same CDS sequence)	NM_001312897.1
Accession Version	NM_001312897.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2352bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	protein-methionine sulfoxide oxidase mical2b
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU914772.16 and AL954703.8. On Aug 21, 2015 this sequence version replaced XM_005170862.2. Transcript Variant: This variant (1) encodes the functional protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01026465.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCTGCTG TGAAAGCGCT TCAACAGTGG TGCAAGATCC AGTGTGAAGG ATACCGGGAC 
GTGTCGATAA CCAACATGAC CACATCTTTC CGGGACGGTT TGGCTTTCTG CGCTCTAATT 
CACAAACACA GACCGGACTT GATAGACTTT GACTCTTTGA GCAAGGAAAA TATTTATGAA 
AACAATCAAC GGGCTTTCGA AGTGGCAGAG AAGGAGTTGG GAATACCTGC CCTCCTGGAT 
GCTGAAGACA TGGTGGCTTT AAAAGTGCCA GATCGCCTTA GTATTCTGAC TTATGTGTCC 
CAGTACTACA ATTATTTCCA CGGGCGCTCT CCCATTGGAG GAATGGGAGG GATTAAAAGA 
CCAGCTGAAG AGTCTAATGA AGCGCCATCT GGGAAGAAGA ACCCACCTGT CATTGCTAAA 
GTTCTTCCCT CACCTAAGCC AGCCACGGAG AATAAAACAC CCAAACCGGC GAATGAAAAT 
AAGAGAGAGG TTTTGACTGA ACGTGCCAAT AAATCGTCAA CTCCGAGCAG CAACTGTAGT 
GTGTGCAACC AACATGTTCA CCTCGTCCAG CGCCACCTAG TAGATGGAAA ACTGTATCAC 
AGGAACTGCT TTAAATGCAA AGAGTGTTCC ACTATTCTCC TCTCTGGCAC CTACAAGGCT 
GGAAAAGAAC CAGGCACTTT CATCTGCAAG ACACACACGA GCATAGAGAA GCCTCTAACT 
ACACGCTTCA CGGCACCATC CAAAACACAA TCCACCTTTA TAAACAAGCC TGACCAGAGT 
GCGTTAGGTG GAAGTACTGG TAAAGCTAGT TTAAATACGA ACACATCTAA ATTGGGTTGG 
CTGGCCAACA AAAGTGACCG CATTCCCATG CGTGGGTTCA CCGACACACC TACTCCAGCA 
GTTCAGCTCA CGCCAACGGC AAAAGCAACA CCCACACCAG ACACTACAAC AGCGAAATCC 
CCCTCAGGCC CCAGATCTGC CACTGAATCT CTTAAACCTG TGAGCAACAC TGTTCAGAAG 
AACCAAGAGG CCAGAATGAG ATTCTTTGAG TCCAGCAGCA GCAGCCCCAG CAGTGTTGTG 
CCTGCCAGTA AGAGCACCTC ATCATACACC TCAACTACAT CAGTGAGTAC TGCAGGTGTT 
AAAACTACAG CACCAGAAAC TACAACACCC AGAGTCCCTG CCGGAGCAGG TAAAGGCAGA 
GTTCTGCTCC GAGTGGGAGA CTGGGCAAAA TCACAGGACA CAGAAAAAGA GAAGGAGAAT 
GAAAAGGAAA AGGAAAAAGC CAAGGCTGTG ATTGGGAAGA TGGTGAAAGT GGACAAAGAT 
AGCAACAGCT CATCTGCTAG TCCAGCTGGG CTGAAAGCAA ACAGCAGTAA TGTTCCCCCT 
CTGGAATCTA ATGCTAAAGC CCCCTTTGGT CGGGTGCGTC TCAAAGCGCT TGAAGCCAGT 
GGTGAAACTA CTGCTCCTGC CACTACAGTC CCATCCTGGA TGAAAGAGAA GAGCTTATCC 
AGGAATGTGA ACACACCGTC ATCTAACAGC AAGGAAAACG ATACGGTTCC ATCTGACTGG 
AGATCAATGC TCAAGCCTGT TTCAAAACCT GTCAGTAATC TAGAAAGCAA GGGCTTACCC 
GCCAGGAGGA CAGAAGCAGT TTCTTCTCCA AAGACATCTG TTCCTGCTTC ATCTACAACA 
ACCATCTCTA TTAGCATCAG CTCCTCCAAA ACACCCTTAA CTACCCCACT GAAAAATGGG 
TCAAAACCTA ATGACTCCAG CAGCGTATTT CCAGTTAGTC CCTCTGGGAC AGAGACGCAC 
AAATCACACA AGCCGGGTTA CATTCCTAAA GAAGACATTC AGAAAGAGCT GAAGGAGATT 
GAGATGAATA TGAACGAGTT GGAGAAAAAA GGAGTAGAGC TGGAAAAACA ACTCCGACAA 
TGTGATGAGG ATGGAGAAGA GGACGCATTA ATGAATGACT TAATGGTGGA CTGGTTTACC 
CTCATCAGAA ACAAGCAGGT CTACATGAGA CGGGAGTCTG AGCTTGTTTA TATTGCCAGG 
ACGCAGGATC TTGAGGAAGA GCAGCCCAGT GTTGATGCCG AACTCCGGAG ACTTATGGAG 
AAACCAGATC ATCTAAAGAC TTTATGGGAT CGTAAACGAG AGGAAGAGCT GATGGCTAAA 
CTGGTGGAGA TTGTTAATGA CCGTAATGCT ATAGTGGAGG GTCTGGATGA AGACAGACTG 
AGGGAGGAAG AGGAGGATGA GCAGCTGAAT AAGATGATGC AGAGTTTTGA TATGAAGAAG 
GAAAAGGTCA AGAAGATGTC TCCAGTAACT CGATGGTTTA GCATGAGAAA ACGGAGGTCT 
TCAGAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001299826.1
CDS	220..2571
Translation	MAAVKALQQWCKIQCEGYRDVSITNMTTSFRDGLAFCALIHKHRPDLIDFDSLSKENIYENNQRAFEVAEKELGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMGGIKRPAEESNEAPSGKKNPPVIAKVLPSPKPATENKTPKPANENKREVLTERANKSSTPSSNCSVCNQHVHLVQRHLVDGKLYHRNCFKCKECSTILLSGTYKAGKEPGTFICKTHTSIEKPLTTRFTAPSKTQSTFINKPDQSALGGSTGKASLNTNTSKLGWLANKSDRIPMRGFTDTPTPAVQLTPTAKATPTPDTTTAKSPSGPRSATESLKPVSNTVQKNQEARMRFFESSSSSPSSVVPASKSTSSYTSTTSVSTAGVKTTAPETTTPRVPAGAGKGRVLLRVGDWAKSQDTEKEKENEKEKEKAKAVIGKMVKVDKDSNSSSASPAGLKANSSNVPPLESNAKAPFGRVRLKALEASGETTAPATTVPSWMKEKSLSRNVNTPSSNSKENDTVPSDWRSMLKPVSKPVSNLESKGLPARRTEAVSSPKTSVPASSTTTISISISSSKTPLTTPLKNGSKPNDSSSVFPVSPSGTETHKSHKPGYIPKEDIQKELKEIEMNMNELEKKGVELEKQLRQCDEDGEEDALMNDLMVDWFTLIRNKQVYMRRESELVYIARTQDLEEEQPSVDAELRRLMEKPDHLKTLWDRKREEELMAKLVEIVNDRNAIVEGLDEDRLREEEEDEQLNKMMQSFDMKKEKVKKMSPVTRWFSMRKRRSSED

Target ORF information:

RefSeq Version	NM_001312897.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio mical-like 2b (micall2b), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001312897.1

ORF Insert Sequence:

ATGGCTGCTG TGAAAGCGCT TCAACAGTGG TGCAAGATCC AGTGTGAAGG ATACCGGGAC 
GTGTCGATAA CCAACATGAC CACATCTTTC CGGGACGGTT TGGCTTTCTG CGCTCTAATT 
CACAAACACA GACCGGACTT GATAGACTTT GACTCTTTGA GCAAGGAAAA TATTTATGAA 
AACAATCAAC GGGCTTTCGA AGTGGCAGAG AAGGAGTTGG GAATACCTGC CCTCCTGGAT 
GCTGAAGACA TGGTGGCTTT AAAAGTGCCA GATCGCCTTA GTATTCTGAC TTATGTGTCC 
CAGTACTACA ATTATTTCCA CGGGCGCTCT CCCATTGGAG GAATGGGAGG GATTAAAAGA 
CCAGCTGAAG AGTCTAATGA AGCGCCATCT GGGAAGAAGA ACCCACCTGT CATTGCTAAA 
GTTCTTCCCT CACCTAAGCC AGCCACGGAG AATAAAACAC CCAAACCGGC GAATGAAAAT 
AAGAGAGAGG TTTTGACTGA ACGTGCCAAT AAATCGTCAA CTCCGAGCAG CAACTGTAGT 
GTGTGCAACC AACATGTTCA CCTCGTCCAG CGCCACCTAG TAGATGGAAA ACTGTATCAC 
AGGAACTGCT TTAAATGCAA AGAGTGTTCC ACTATTCTCC TCTCTGGCAC CTACAAGGCT 
GGAAAAGAAC CAGGCACTTT CATCTGCAAG ACACACACGA GCATAGAGAA GCCTCTAACT 
ACACGCTTCA CGGCACCATC CAAAACACAA TCCACCTTTA TAAACAAGCC TGACCAGAGT 
GCGTTAGGTG GAAGTACTGG TAAAGCTAGT TTAAATACGA ACACATCTAA ATTGGGTTGG 
CTGGCCAACA AAAGTGACCG CATTCCCATG CGTGGGTTCA CCGACACACC TACTCCAGCA 
GTTCAGCTCA CGCCAACGGC AAAAGCAACA CCCACACCAG ACACTACAAC AGCGAAATCC 
CCCTCAGGCC CCAGATCTGC CACTGAATCT CTTAAACCTG TGAGCAACAC TGTTCAGAAG 
AACCAAGAGG CCAGAATGAG ATTCTTTGAG TCCAGCAGCA GCAGCCCCAG CAGTGTTGTG 
CCTGCCAGTA AGAGCACCTC ATCATACACC TCAACTACAT CAGTGAGTAC TGCAGGTGTT 
AAAACTACAG CACCAGAAAC TACAACACCC AGAGTCCCTG CCGGAGCAGG TAAAGGCAGA 
GTTCTGCTCC GAGTGGGAGA CTGGGCAAAA TCACAGGACA CAGAAAAAGA GAAGGAGAAT 
GAAAAGGAAA AGGAAAAAGC CAAGGCTGTG ATTGGGAAGA TGGTGAAAGT GGACAAAGAT 
AGCAACAGCT CATCTGCTAG TCCAGCTGGG CTGAAAGCAA ACAGCAGTAA TGTTCCCCCT 
CTGGAATCTA ATGCTAAAGC CCCCTTTGGT CGGGTGCGTC TCAAAGCGCT TGAAGCCAGT 
GGTGAAACTA CTGCTCCTGC CACTACAGTC CCATCCTGGA TGAAAGAGAA GAGCTTATCC 
AGGAATGTGA ACACACCGTC ATCTAACAGC AAGGAAAACG ATACGGTTCC ATCTGACTGG 
AGATCAATGC TCAAGCCTGT TTCAAAACCT GTCAGTAATC TAGAAAGCAA GGGCTTACCC 
GCCAGGAGGA CAGAAGCAGT TTCTTCTCCA AAGACATCTG TTCCTGCTTC ATCTACAACA 
ACCATCTCTA TTAGCATCAG CTCCTCCAAA ACACCCTTAA CTACCCCACT GAAAAATGGG 
TCAAAACCTA ATGACTCCAG CAGCGTATTT CCAGTTAGTC CCTCTGGGAC AGAGACGCAC 
AAATCACACA AGCCGGGTTA CATTCCTAAA GAAGACATTC AGAAAGAGCT GAAGGAGATT 
GAGATGAATA TGAACGAGTT GGAGAAAAAA GGAGTAGAGC TGGAAAAACA ACTCCGACAA 
TGTGATGAGG ATGGAGAAGA GGACGCATTA ATGAATGACT TAATGGTGGA CTGGTTTACC 
CTCATCAGAA ACAAGCAGGT CTACATGAGA CGGGAGTCTG AGCTTGTTTA TATTGCCAGG 
ACGCAGGATC TTGAGGAAGA GCAGCCCAGT GTTGATGCCG AACTCCGGAG ACTTATGGAG 
AAACCAGATC ATCTAAAGAC TTTATGGGAT CGTAAACGAG AGGAAGAGCT GATGGCTAAA 
CTGGTGGAGA TTGTTAATGA CCGTAATGCT ATAGTGGAGG GTCTGGATGA AGACAGACTG 
AGGGAGGAAG AGGAGGATGA GCAGCTGAAT AAGATGATGC AGAGTTTTGA TATGAAGAAG 
GAAAAGGTCA AGAAGATGTC TCCAGTAACT CGATGGTTTA GCATGAGAAA ACGGAGGTCT 
TCAGAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

micall2b ( XM_017354911.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017210400.1 Danio rerio mical-like 2b (micall2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa53702
Clone ID Related Accession (Same CDS sequence)	XM_017354911.1
Accession Version	XM_017354911.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2442bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein-methionine sulfoxide oxidase mical2b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG TGAAAGCGCT TCAACAGTGG TGCAAGATCC AGTGTGAAGG ATACCGGGAC 
GTGTCGATAA CCAACATGAC CACATCTTTC CGGGACGGTT TGGCTTTCTG CGCTCTAATT 
CACAAACACA GACCGGACTT GATAGACTTT GACTCTTTGA GCAAGGAAAA TATTTATGAA 
AACAATCAAC GGGCTTTCGA AGTGGCAGAG AAGGAGTTGG GAATACCTGC CCTCCTGGAT 
GCTGAAGACA TGGTGGCTTT AAAAGTGCCA GATCGCCTTA GTATTCTGAC TTATGTGTCC 
CAGTACTACA ATTATTTCCA CGGGCGCTCT CCCATTGGAG GAATGGGAGG GATTAAAAGA 
CCAGCTGAAG AGTCTAATGA AGCGCCATCT GGGAAGAAGA ACCCACCTGT CATTGCTAAA 
GTTCTTCCCT CACCTAAGCC AGCCACGGAG AATAAAACAC CCAAACCGGC GAATGAAAAT 
AAGAGAGAGG TTTTGACTGA ACGTGCCAAT AAATCGTCAA CTCCGAGCAG CAACTGTAGT 
GTGTGCAACC AACATGTTCA CCTCGTCCAG CGCCACCTAG TAGATGGAAA ACTGTATCAC 
AGGAACTGCT TTAAATGCAA AGAGTGTTCC ACTATTCTCC TCTCTGGCAC CTACAAGGCT 
GGAAAAGAAC CAGGCACTTT CATCTGCAAG ACACACACGA GCATAGAGAA GCCTCTAACT 
ACACGCTTCA CGGCACCATC CAAAACACAA TCCACCTTTA TAAACAAGCC TGACCAGAGT 
GCGTTAGGTG GAAGTACTGG TAAAGCTAGT TTAAATACGA ACACATCTAA ATTGGGTTGG 
CTGGCCAACA AAAGTGACCG CATTCCCATG CGTGGGTTCA CCGACACACC TACTCCAGCA 
GTTCAGCTCA CGCCAACGGC AAAAGCAACA CCCACACCAG ACACTACAAC AGCGAAATCC 
CCCTCAGGCC CCAGATCTGC CACTGAATCT CTTAAACCTG TGAGCAACAC TGTTCAGAAG 
AACCAAGAGG CCAGAATGAG ATTCTTTGAG TCCAGCAGCA GCAGCCCCAG CAGTGTTGTG 
CCTGCCAGTA AGAGCACCTC ATCATACACC TCAACTACAT CAGTGAGTAC TGCAGGTGTT 
AAAACTACAG CACCAGAAAC TACAACACCC AGAGTCCCTG CCGGAGCAGG TAAAGGCAGA 
GTTCTGCTCC GAGTGGGAGA CTGGGCAAAA TCACAGGACA CAGAAAAAGA GAAGGAGAAT 
GAAAAGGAAA AGGAAAAAGC CAAGGCTGTG ATTGGGAAGA TGGTGAAAGT GGACAAAGAT 
AGCAACAGCT CATCTGCTAG TCCAGCTGGG CTGAAAGCAA ACAGCAGTAA TGTTCCCCCT 
CTGGAATCTA ATGCTAAAGC CCCCTTTGGT CGGGTGCGTC TCAAAGCGCT TGAAGCCAGT 
GGTGAAACTA CTGCTCCTGC CACTACAGTC CCATCCTGGA TGAAAGAGAA GAGCTTATCC 
AGGAATGTGA ACACACCGTC ATCTAACAGC AAGGAAAACG ATACGGTTCC ATCTGACTGG 
AGATCAATGC TCAAGCCTGT TTCAAAACGC TCTGTTCCTC CCACTTCTTC AAGCTCTGCT 
GGGATTGGCT GTCATGATTT GCGTGTCAAG CCGCTGCGTC CTATAAGAGC AGCGAGACCT 
GTCAGTAATC TAGAAAGCAA GGGCTTACCC GCCAGGAGGA CAGAAGCAGT TTCTTCTCCA 
AAGACATCTG TTCCTGCTTC ATCTACAACA ACCATCTCTA TTAGCATCAG CTCCTCCAAA 
ACACCCTTAA CTACCCCACT GAAAAATGGG TCAAAACCTA ATGACTCCAG CAGCGTATTT 
CCAGTTAGTC CCTCTGGGAC AGAGACGCAC AAATCACACA AGCCGGGTTA CATTCCTAAA 
GAAGACATTC AGAAAGAGCT GAAGGAGATT GAGATGAATA TGAACGAGTT GGAGAAAAAA 
GGAGTAGAGC TGGAAAAACA ACTCCGACAA TGTGATGAGG ATGGAGAAGA GGACGCATTA 
ATGAATGACT TAATGGTGGA CTGGTTTACC CTCATCAGAA ACAAGCAGGT CTACATGAGA 
CGGGAGTCTG AGCTTGTTTA TATTGCCAGG ACGCAGGATC TTGAGGAAGA GCAGCCCAGT 
GTTGATGCCG AACTCCGGAG ACTTATGGAG AAACCAGATC ATCTAAAGAC TTTATGGGAT 
CGTAAACGAG AGGAAGAGCT GATGGCTAAA CTGGTGGAGA TTGTTAATGA CCGTAATGCT 
ATAGTGGAGG GTCTGGATGA AGACAGACTG AGGGAGGAAG AGGAGGATGA GCAGCTGAAT 
AAGATGATGC AGAGTTTTGA TATGAAGAAG GAAAAGGTCA AGAAGATGTC TCCAGTAACT 
CGATGGTTTA GCATGAGAAA ACGGAGGTCT TCAGAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017210400.1
CDS	207..2648
Translation	MAAVKALQQWCKIQCEGYRDVSITNMTTSFRDGLAFCALIHKHRPDLIDFDSLSKENIYENNQRAFEVAEKELGIPALLDAEDMVALKVPDRLSILTYVSQYYNYFHGRSPIGGMGGIKRPAEESNEAPSGKKNPPVIAKVLPSPKPATENKTPKPANENKREVLTERANKSSTPSSNCSVCNQHVHLVQRHLVDGKLYHRNCFKCKECSTILLSGTYKAGKEPGTFICKTHTSIEKPLTTRFTAPSKTQSTFINKPDQSALGGSTGKASLNTNTSKLGWLANKSDRIPMRGFTDTPTPAVQLTPTAKATPTPDTTTAKSPSGPRSATESLKPVSNTVQKNQEARMRFFESSSSSPSSVVPASKSTSSYTSTTSVSTAGVKTTAPETTTPRVPAGAGKGRVLLRVGDWAKSQDTEKEKENEKEKEKAKAVIGKMVKVDKDSNSSSASPAGLKANSSNVPPLESNAKAPFGRVRLKALEASGETTAPATTVPSWMKEKSLSRNVNTPSSNSKENDTVPSDWRSMLKPVSKRSVPPTSSSSAGIGCHDLRVKPLRPIRAARPVSNLESKGLPARRTEAVSSPKTSVPASSTTTISISISSSKTPLTTPLKNGSKPNDSSSVFPVSPSGTETHKSHKPGYIPKEDIQKELKEIEMNMNELEKKGVELEKQLRQCDEDGEEDALMNDLMVDWFTLIRNKQVYMRRESELVYIARTQDLEEEQPSVDAELRRLMEKPDHLKTLWDRKREEELMAKLVEIVNDRNAIVEGLDEDRLREEEEDEQLNKMMQSFDMKKEKVKKMSPVTRWFSMRKRRSSED

Target ORF information:

RefSeq Version	XM_017354911.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio mical-like 2b (micall2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354911.1

ORF Insert Sequence:

ATGGCTGCTG TGAAAGCGCT TCAACAGTGG TGCAAGATCC AGTGTGAAGG ATACCGGGAC 
GTGTCGATAA CCAACATGAC CACATCTTTC CGGGACGGTT TGGCTTTCTG CGCTCTAATT 
CACAAACACA GACCGGACTT GATAGACTTT GACTCTTTGA GCAAGGAAAA TATTTATGAA 
AACAATCAAC GGGCTTTCGA AGTGGCAGAG AAGGAGTTGG GAATACCTGC CCTCCTGGAT 
GCTGAAGACA TGGTGGCTTT AAAAGTGCCA GATCGCCTTA GTATTCTGAC TTATGTGTCC 
CAGTACTACA ATTATTTCCA CGGGCGCTCT CCCATTGGAG GAATGGGAGG GATTAAAAGA 
CCAGCTGAAG AGTCTAATGA AGCGCCATCT GGGAAGAAGA ACCCACCTGT CATTGCTAAA 
GTTCTTCCCT CACCTAAGCC AGCCACGGAG AATAAAACAC CCAAACCGGC GAATGAAAAT 
AAGAGAGAGG TTTTGACTGA ACGTGCCAAT AAATCGTCAA CTCCGAGCAG CAACTGTAGT 
GTGTGCAACC AACATGTTCA CCTCGTCCAG CGCCACCTAG TAGATGGAAA ACTGTATCAC 
AGGAACTGCT TTAAATGCAA AGAGTGTTCC ACTATTCTCC TCTCTGGCAC CTACAAGGCT 
GGAAAAGAAC CAGGCACTTT CATCTGCAAG ACACACACGA GCATAGAGAA GCCTCTAACT 
ACACGCTTCA CGGCACCATC CAAAACACAA TCCACCTTTA TAAACAAGCC TGACCAGAGT 
GCGTTAGGTG GAAGTACTGG TAAAGCTAGT TTAAATACGA ACACATCTAA ATTGGGTTGG 
CTGGCCAACA AAAGTGACCG CATTCCCATG CGTGGGTTCA CCGACACACC TACTCCAGCA 
GTTCAGCTCA CGCCAACGGC AAAAGCAACA CCCACACCAG ACACTACAAC AGCGAAATCC 
CCCTCAGGCC CCAGATCTGC CACTGAATCT CTTAAACCTG TGAGCAACAC TGTTCAGAAG 
AACCAAGAGG CCAGAATGAG ATTCTTTGAG TCCAGCAGCA GCAGCCCCAG CAGTGTTGTG 
CCTGCCAGTA AGAGCACCTC ATCATACACC TCAACTACAT CAGTGAGTAC TGCAGGTGTT 
AAAACTACAG CACCAGAAAC TACAACACCC AGAGTCCCTG CCGGAGCAGG TAAAGGCAGA 
GTTCTGCTCC GAGTGGGAGA CTGGGCAAAA TCACAGGACA CAGAAAAAGA GAAGGAGAAT 
GAAAAGGAAA AGGAAAAAGC CAAGGCTGTG ATTGGGAAGA TGGTGAAAGT GGACAAAGAT 
AGCAACAGCT CATCTGCTAG TCCAGCTGGG CTGAAAGCAA ACAGCAGTAA TGTTCCCCCT 
CTGGAATCTA ATGCTAAAGC CCCCTTTGGT CGGGTGCGTC TCAAAGCGCT TGAAGCCAGT 
GGTGAAACTA CTGCTCCTGC CACTACAGTC CCATCCTGGA TGAAAGAGAA GAGCTTATCC 
AGGAATGTGA ACACACCGTC ATCTAACAGC AAGGAAAACG ATACGGTTCC ATCTGACTGG 
AGATCAATGC TCAAGCCTGT TTCAAAACGC TCTGTTCCTC CCACTTCTTC AAGCTCTGCT 
GGGATTGGCT GTCATGATTT GCGTGTCAAG CCGCTGCGTC CTATAAGAGC AGCGAGACCT 
GTCAGTAATC TAGAAAGCAA GGGCTTACCC GCCAGGAGGA CAGAAGCAGT TTCTTCTCCA 
AAGACATCTG TTCCTGCTTC ATCTACAACA ACCATCTCTA TTAGCATCAG CTCCTCCAAA 
ACACCCTTAA CTACCCCACT GAAAAATGGG TCAAAACCTA ATGACTCCAG CAGCGTATTT 
CCAGTTAGTC CCTCTGGGAC AGAGACGCAC AAATCACACA AGCCGGGTTA CATTCCTAAA 
GAAGACATTC AGAAAGAGCT GAAGGAGATT GAGATGAATA TGAACGAGTT GGAGAAAAAA 
GGAGTAGAGC TGGAAAAACA ACTCCGACAA TGTGATGAGG ATGGAGAAGA GGACGCATTA 
ATGAATGACT TAATGGTGGA CTGGTTTACC CTCATCAGAA ACAAGCAGGT CTACATGAGA 
CGGGAGTCTG AGCTTGTTTA TATTGCCAGG ACGCAGGATC TTGAGGAAGA GCAGCCCAGT 
GTTGATGCCG AACTCCGGAG ACTTATGGAG AAACCAGATC ATCTAAAGAC TTTATGGGAT 
CGTAAACGAG AGGAAGAGCT GATGGCTAAA CTGGTGGAGA TTGTTAATGA CCGTAATGCT 
ATAGTGGAGG GTCTGGATGA AGACAGACTG AGGGAGGAAG AGGAGGATGA GCAGCTGAAT 
AAGATGATGC AGAGTTTTGA TATGAAGAAG GAAAAGGTCA AGAAGATGTC TCCAGTAACT 
CGATGGTTTA GCATGAGAAA ACGGAGGTCT TCAGAAGATT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A new locus regulating MICALL2 expression was identified for association with executive inhibition in children with attention deficit hyperactivity disorder.
Molecular psychiatry(2017 Apr)
Yang L,Chang S,Lu Q,Zhang Y,Wu Z,Sun X,Cao Q,Qian Y,Jia T,Xu B,Duan Q,Li Y,Zhang K,Schumann G,Liu D,Wang J,Wang Y,Lu L

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Novel cardiovascular gene functions revealed via systematic phenotype prediction in zebrafish.
Development (Cambridge, England)141(1)224-35(2014 Jan)
Musso G,Tasan M,Mosimann C,Beaver JE,Plovie E,Carr LA,Chua HN,Dunham J,Zuberi K,Rodriguez H,Morris Q,Zon L,Roth FP,MacRae CA

Identification and expression analysis of mical family genes in zebrafish.
Journal of genetics and genomics = Yi chuan xue bao37(10)685-93(2010 Oct)
Xue Y,Kuok C,Xiao A,Zhu Z,Lin S,Zhang B

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micall2b cDNA ORF clone, Danio rerio(zebrafish)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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