dennd5b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	dennd5b
Entrez Gene ID	323528
Full Name	DENN/MADD domain containing 5B
Synonyms	id:ibd5082,wu:fc01g04,zgc:77218
General protein information	Preferred Names DENN/MADD domain containing 5B Names DENN domain-containing protein 5B RAB6IP1-like protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005164712.4	XP_005164769.1	DENN domain-containing protein 5B isoform X2
XM_009300252.3	XP_009298527.1	DENN domain-containing protein 5B isoform X4
XM_005164711.3	XP_005164768.1	DENN domain-containing protein 5B isoform X1
XM_005164711.4	XP_005164768.1	DENN domain-containing protein 5B isoform X1
XM_005164712.3	XP_005164769.1	DENN domain-containing protein 5B isoform X2
XM_009300252.2	XP_009298527.1	DENN domain-containing protein 5B isoform X3
NM_212644.1	NP_997809.1	DENN domain-containing protein 5B
XM_021474931.1	XP_021330606.1	DENN domain-containing protein 5B isoform X3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	DENND5B	XP_001137996.1
Bos taurus (cattle)	DENND5B	NP_001192417.1
Gallus gallus (chicken)	DENND5B	XP_004937958.1
Homo sapiens (human)	DENND5B	NP_659410.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011351	XP_564133.3
	DENND5B	XP_002798573.1
Mus musculus (house mouse)	Dennd5b	NP_796166.2
Rattus norvegicus (Norway rat)	Dennd5b	XP_006237774.1
Canis lupus familiaris (dog)	DENND5B	XP_005637062.1
Danio rerio (zebrafish)	dennd5b	NP_997809.1
Drosophila melanogaster (fruit fly)	CG7852	NP_647620.1
Xenopus tropicalis (tropical clawed frog)	dennd5b	NP_001135644.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

A gene expression screen in zebrafish embryogenesis.
Kudoh T, Tsang M, Hukriede NA, Chen X, Dedekian M, Clarke CJ, Kiang A, Schultz S, Epstein JA, Toyama R, Dawid IB
Genome research11(12)1979-87(2001 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following dennd5b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dennd5b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141945	XM_021474931.1 Latest version!	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa37335	NM_212644.1 Latest version!	Danio rerio DENN/MADD domain containing 5B (dennd5b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa09340	XM_005164711.4 Latest version!	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa09341	XM_005164712.4 Latest version!	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa39770	XM_009300252.3 Latest version!	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa09340	XM_005164711.3	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa09341	XM_005164712.3	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa39770	XM_009300252.2	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dennd5b ( XM_021474931.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330606.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa141945
Clone ID Related Accession (Same CDS sequence)	XM_021474931.1
Accession Version	XM_021474931.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2088bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCTCCTG ATGAATCATA CTCTGTGCAA AGCAATCTGC CTGTTTTCAT TTCTTCATTT 
GTCTCCATTT TCTTTAACTT CTTGCTTGTC TTATCTTGTC TGACTGCCTG GCGTTCATTA 
GCATGCTGCA TTTTTCTTTT CTATTGTTTC TTCACCCTGT CTGTGTGTGT GTTTTTCGTT 
GGGATGCTGT GGGGTGTGGG TCGTACCACC TCGCAACAGG TGGAGGGGTG TTTGCTCCTG 
CAGAACTCTA TGGCTTTCTG GGAGTGGGAT AAGGCGCCAC CCCCGCCTGT TAAACCACCT 
AAAAAGTCCT TCTCGGCCTG CTGTCTGAAG TACATGCAGG AGGCTCGTAA CCTGGGGAAG 
AACCTGCGAC AACCCAAATT ATCAGACTTG TCCCCTGCCG TCATCGCACA GACCAACTGG 
AAGTTTGTGG AGGGTTTACT GAAGGAATGC AAGATGAAGA CCAAGCGCAT GTTGGTGGAG 
AAAATGGGGA GAGAAGCTGT CGAACTGGGT CACGGAGAGG CAAACATCAC TGGTCTGGAA 
GAAAACACAC TCATAGCCAG TCTGTGTGAC CTGCTGGAGA GAATATGGAG CCACGGGCTT 
CAGGTTAAAC AGGGAAAGTC TGCACTTTGG TCTCATTTAT TGCATTACCA GGCGAGAGAA 
GAGAAAAACG AACAGCAGTT GGAGTCTCCG GTGTCTAATG GCCAGGAAAG ACGGAAAACC 
GAGTCCAGCG TTGGTCTTCC TGGATTACGT GTGTCCGTCA TACAGGATAT GAGACACATC 
CAGAACATGG CAGAGATTAA GACTGATGTA GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT 
CTGGAGAAAA AACTGCTTTC CCAGCACCTC AAACAGCTGC TGTCAAACCA GGCCTTAACC 
AAGAAGCTCT ACAAACGTTA CGCTTTTCTC CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC 
TTTCATCTCC TGAGCCTGAA TGCTGTGGAC TACTTCTGCT TTACCAGCGT CTTCACTACT 
ATAATGATCC CATACCGGGC TGTGATCATC CCCATCAAGA AGCTGAGTAA CGCCATGACC 
ACGTCCAACC CCTGGCTGTG TGTGTCGGGC GAGCTGGGCG ACTCCGGCAT CCTGCAGATC 
ACCAAAAACA TACTGGAGAT GACATTCGAC TCTGCCTTTA GGCCTCACAC GCACATCTCC 
ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG CTGACCACTG TTCAGCTGGG TCATGATAAC 
TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT TGCGTCATGG TCCGCAATGA GATCACCGGA 
CACACATACA AGTTTCCCTG TGGCCGGTGG CTGGGAAAGG GTGTGGATGA TGGCAGTCTG 
GAGCGAGTGC TGATTGGAGA GTTTGTGGTG CCTTGCGGCG ATGATGAAGG AGGTCGAGGG 
TCTAAAACAC CACCGCTCCA GAGGTCGCCC TCCCAGATCC GGAGAATCAG CATCACTTCA 
CTCACCGGAA AAGGAAACAA ACCAACAACC GTTCAGATCC AGGAATCTAT CGGTGAAGCA 
GTCAACAATA TAATTAAACA TTTCCACAAA CCAGAGAAAG AGCGGGGCAG TCTCACGGTT 
CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG GCGCTGGAGC AGTTTTTCCA CCATGGGTTT 
CGCTCTGCTC GAATCTTCCA GAAAAATGTC TTTGTTTGGG ATTTCTTTGA GAGAATAGTA 
GCGTTTATGG AGAACAGAGA TCAAACTGGA GACCTGCAAG AATCCCCCGA GCCTCTCAGC 
CTGAATAGCG AATCACTCTG TCGATACGTC AACGCCATCA ACAACATGCC ACGCAACATC 
GGCAAAGACG GCAAATTCCA GCTGCTAGTT TGCCTCGGCG CGAGAGACCG ATTATTACCC 
CAGTGGCTCC CTCTACTGGC TGAAAGCCCG GTCATCACGC GTATGTACGA AGAAAACGCT 
TTACTTCGAG ACAAACTCAC CGTAAGCTCT CTCCTCGGCG TCTTAGAAAC GCTACATGAT 
TTCCCCATTA CCCTGGAGAC TTCGCTTACA AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330606.1
CDS	3..2090
Translation	MSPDESYSVQSNLPVFISSFVSIFFNFLLVLSCLTAWRSLACCIFLFYCFFTLSVCVFFVGMLWGVGRTTSQQVEGCLLLQNSMAFWEWDKAPPPPVKPPKKSFSACCLKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDSAFRPHTHISIAFKCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	XM_021474931.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474931.1

ORF Insert Sequence:

ATGTCTCCTG ATGAATCATA CTCTGTGCAA AGCAATCTGC CTGTTTTCAT TTCTTCATTT 
GTCTCCATTT TCTTTAACTT CTTGCTTGTC TTATCTTGTC TGACTGCCTG GCGTTCATTA 
GCATGCTGCA TTTTTCTTTT CTATTGTTTC TTCACCCTGT CTGTGTGTGT GTTTTTCGTT 
GGGATGCTGT GGGGTGTGGG TCGTACCACC TCGCAACAGG TGGAGGGGTG TTTGCTCCTG 
CAGAACTCTA TGGCTTTCTG GGAGTGGGAT AAGGCGCCAC CCCCGCCTGT TAAACCACCT 
AAAAAGTCCT TCTCGGCCTG CTGTCTGAAG TACATGCAGG AGGCTCGTAA CCTGGGGAAG 
AACCTGCGAC AACCCAAATT ATCAGACTTG TCCCCTGCCG TCATCGCACA GACCAACTGG 
AAGTTTGTGG AGGGTTTACT GAAGGAATGC AAGATGAAGA CCAAGCGCAT GTTGGTGGAG 
AAAATGGGGA GAGAAGCTGT CGAACTGGGT CACGGAGAGG CAAACATCAC TGGTCTGGAA 
GAAAACACAC TCATAGCCAG TCTGTGTGAC CTGCTGGAGA GAATATGGAG CCACGGGCTT 
CAGGTTAAAC AGGGAAAGTC TGCACTTTGG TCTCATTTAT TGCATTACCA GGCGAGAGAA 
GAGAAAAACG AACAGCAGTT GGAGTCTCCG GTGTCTAATG GCCAGGAAAG ACGGAAAACC 
GAGTCCAGCG TTGGTCTTCC TGGATTACGT GTGTCCGTCA TACAGGATAT GAGACACATC 
CAGAACATGG CAGAGATTAA GACTGATGTA GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT 
CTGGAGAAAA AACTGCTTTC CCAGCACCTC AAACAGCTGC TGTCAAACCA GGCCTTAACC 
AAGAAGCTCT ACAAACGTTA CGCTTTTCTC CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC 
TTTCATCTCC TGAGCCTGAA TGCTGTGGAC TACTTCTGCT TTACCAGCGT CTTCACTACT 
ATAATGATCC CATACCGGGC TGTGATCATC CCCATCAAGA AGCTGAGTAA CGCCATGACC 
ACGTCCAACC CCTGGCTGTG TGTGTCGGGC GAGCTGGGCG ACTCCGGCAT CCTGCAGATC 
ACCAAAAACA TACTGGAGAT GACATTCGAC TCTGCCTTTA GGCCTCACAC GCACATCTCC 
ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG CTGACCACTG TTCAGCTGGG TCATGATAAC 
TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT TGCGTCATGG TCCGCAATGA GATCACCGGA 
CACACATACA AGTTTCCCTG TGGCCGGTGG CTGGGAAAGG GTGTGGATGA TGGCAGTCTG 
GAGCGAGTGC TGATTGGAGA GTTTGTGGTG CCTTGCGGCG ATGATGAAGG AGGTCGAGGG 
TCTAAAACAC CACCGCTCCA GAGGTCGCCC TCCCAGATCC GGAGAATCAG CATCACTTCA 
CTCACCGGAA AAGGAAACAA ACCAACAACC GTTCAGATCC AGGAATCTAT CGGTGAAGCA 
GTCAACAATA TAATTAAACA TTTCCACAAA CCAGAGAAAG AGCGGGGCAG TCTCACGGTT 
CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG GCGCTGGAGC AGTTTTTCCA CCATGGGTTT 
CGCTCTGCTC GAATCTTCCA GAAAAATGTC TTTGTTTGGG ATTTCTTTGA GAGAATAGTA 
GCGTTTATGG AGAACAGAGA TCAAACTGGA GACCTGCAAG AATCCCCCGA GCCTCTCAGC 
CTGAATAGCG AATCACTCTG TCGATACGTC AACGCCATCA ACAACATGCC ACGCAACATC 
GGCAAAGACG GCAAATTCCA GCTGCTAGTT TGCCTCGGCG CGAGAGACCG ATTATTACCC 
CAGTGGCTCC CTCTACTGGC TGAAAGCCCG GTCATCACGC GTATGTACGA AGAAAACGCT 
TTACTTCGAG ACAAACTCAC CGTAAGCTCT CTCCTCGGCG TCTTAGAAAC GCTACATGAT 
TTCCCCATTA CCCTGGAGAC TTCGCTTACA AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( NM_212644.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_997809.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), mRNA.

Price & Availability↑

CloneID	ODa37335
Clone ID Related Accession (Same CDS sequence)	NM_212644.1
Accession Version	NM_212644.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3936bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5B
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC067136.1. ##Evidence-Data-START## Transcript exon combination :: BC067136.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGC TTTATACCAG 
TGGCTAGAGG CTGATCGGCA GGGCAAGGAT CCAGAGGCCA CAGCAGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCTT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAAAA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TGCCAGAATC TGGGCAAGCT GACCACTGTT CAGCTGGGTC ATGATAACTC CGGCTTGCTG 
GCCAAGTGGC TGGTTGATTG CGTCATGGTC CGCAATGAGA TCACCGGACA CACATACAAG 
TTTCCCTGTG GCCGGTGGCT GGGAAAGGGG GTGGATGATG GCAGTCTGGA GCGAGTGCTG 
ATTGGAGAGT TTGTGGTGCC TTGCGGCGAT GATGAAGGAG GTCGAGGGTC TAAAACACCA 
CCGCTCCAGA GGTCGCCCTC CCAGATCCGG AGAATCAGCA TCACTTCACT CACCGGAAAA 
GGAAACAAAC CAACAACCGT TCAGATCCAG GAATCTATCG GTGAAGCAGT CAACAATATA 
ATTAAACATT TCCACAAACC AGAGAAAGAG CGGGGCAGTC TCACGGTTCT GCTGTGTGGA 
GAAGGTGGTC TTGTTTGGGC GCTGGAGCAG TTTTTCCACC ATGGGTTTCG CTCTGCTCGA 
ATCTTCCAGA AAAATGTCTT TGTTTGGGAT TTCTTTGAGA GAATAGTAGC GTTTATGGAG 
AACAGAGATC AAACTGGAGA CCTGCAAGAA TCCCCCGAGC CTCTCAGCCT GAATAGCGAA 
TCACTCTGTC GATACGTCAA CGCCATCAAC AACATGCCAC GCAACATCGG CAAAGACGGC 
AAATTCCAGC TGCTAGTTTG CCTCGGCGCG AGAGACCGAT TATTACCCCA GTGGCTCCCT 
CTACTGGCTG AAAGCCCGGT CATCACGCGT ATGTACGAAG AAAACGCTTT ACTTCGAGAC 
AAACTCACCG TAAGCTCTCT CCTCGGCGTC TTAGAAACGC TACATGATTT CCCCATTACC 
CTGGAGACTT CGCTTACAAA AAGCGTCGAG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_997809.1
CDS	166..4101
Translation	MSATMSGSGSAPCRFAHYFIVCGLDDETGLEPDALAALYQWLEADRQGKDPEATAAGENFDQSPLKRTFKSKVLAHYPENIECNPFDQDAVNMLCMPKGLSFRTQRDSLAPHFHSFLITREDGSRTYGFVHTFYEEVTSLQICSAMQTLHQMHQAENNTSAQNCTSSSSSSSSSSSSSSMDSLSSSLDDVDSPSAHGGRRTCEGYDSIRDTLYVSKAMCLIAPMPFMHACKRFLAQMHRAVSCSPPPPLPLESYIYNVLYEVPLPASGRSLKFHGVYEPILCQRPGVGELPLADFPLADAFRLLGVENLVQLFTCVLLEMQILLYSQDYQRLMVVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHCIELPEDFPQFPNRSEFIQELSEVLLSFGQSPEGGSSSPEPAVMSLQTSVLEKELKSTSLRELVDDKTNGNLGGEALDVLELLQGNPTLERLQALAKRTGVKVARLEALAAGQKGDEGVGGRSPVEEEELRNAKLNVQLREVFAARFATMFADYESFVIQNSPDLESWLTNREQMHNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKEPLLRVFDGRIEKARLYNVRAPSLRSSVYQKCTFLKESAQAIEQRLRKIDHTAIHPHLLDMKIGQGKYQQGFFPKLQADVLASGPTSNKWSNRTCSTQRRVERHRQQNEHLVLDNELKEKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	NM_212644.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212644.1

ORF Insert Sequence:

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGC TTTATACCAG 
TGGCTAGAGG CTGATCGGCA GGGCAAGGAT CCAGAGGCCA CAGCAGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCTT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAAAA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TGCCAGAATC TGGGCAAGCT GACCACTGTT CAGCTGGGTC ATGATAACTC CGGCTTGCTG 
GCCAAGTGGC TGGTTGATTG CGTCATGGTC CGCAATGAGA TCACCGGACA CACATACAAG 
TTTCCCTGTG GCCGGTGGCT GGGAAAGGGG GTGGATGATG GCAGTCTGGA GCGAGTGCTG 
ATTGGAGAGT TTGTGGTGCC TTGCGGCGAT GATGAAGGAG GTCGAGGGTC TAAAACACCA 
CCGCTCCAGA GGTCGCCCTC CCAGATCCGG AGAATCAGCA TCACTTCACT CACCGGAAAA 
GGAAACAAAC CAACAACCGT TCAGATCCAG GAATCTATCG GTGAAGCAGT CAACAATATA 
ATTAAACATT TCCACAAACC AGAGAAAGAG CGGGGCAGTC TCACGGTTCT GCTGTGTGGA 
GAAGGTGGTC TTGTTTGGGC GCTGGAGCAG TTTTTCCACC ATGGGTTTCG CTCTGCTCGA 
ATCTTCCAGA AAAATGTCTT TGTTTGGGAT TTCTTTGAGA GAATAGTAGC GTTTATGGAG 
AACAGAGATC AAACTGGAGA CCTGCAAGAA TCCCCCGAGC CTCTCAGCCT GAATAGCGAA 
TCACTCTGTC GATACGTCAA CGCCATCAAC AACATGCCAC GCAACATCGG CAAAGACGGC 
AAATTCCAGC TGCTAGTTTG CCTCGGCGCG AGAGACCGAT TATTACCCCA GTGGCTCCCT 
CTACTGGCTG AAAGCCCGGT CATCACGCGT ATGTACGAAG AAAACGCTTT ACTTCGAGAC 
AAACTCACCG TAAGCTCTCT CCTCGGCGTC TTAGAAACGC TACATGATTT CCCCATTACC 
CTGGAGACTT CGCTTACAAA AAGCGTCGAG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_005164711.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164768.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.

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CloneID	ODa09340
Clone ID Related Accession (Same CDS sequence)	XM_005164711.3 , XM_005164711.4
Accession Version	XM_005164711.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 5B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164711.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGC TTTATACCAG 
TGGCTAGAGG CTGATCGGCA GGGCAAGGAT CCAGAGGCCA CAGCAGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164768.1
CDS	283..4260
Translation	MSATMSGSGSAPCRFAHYFIVCGLDDETGLEPDALAALYQWLEADRQGKDPEATAAGENFDQSPLKRTFKSKVLAHYPENIECNPFDQDAVNMLCMPKGLSFRTQRDSLAPHFHSFLITREDGSRTYGFVHTFYEEVTSPQICSAMQTLHQMHQAEHNTSAQNCTSSSSSSSSSSSSSSMDSLSSSLDDVDSPSAHGGRRTCEGYDSIRDTLYVSKAMCLIAPMPFMHACKRFLAQMHRAVSCSPPPPLPLESYIYNVLYEVPLPASGRSLKFHGVYEPILCQRPGVGELPLADFPLADAFRLLGVENLVQLFTCVLLEMQILLYSQDYQRLMVVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHCIELPEDFPQFPNRSEFIQELSEVLLSFGQSPEGGSSSPEPAVMSLQTSVLEKELKSTSLRELVDDKTNGNLGGEALDVLELLQGNPTLERLQALAKRTGVKVARLEALAAGQKGDEGVGGRSPVEEEELRNAKLNVQLREVFAARFATMFADYESFVIQNSPDLESWLTNREQMHNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKEPLLRVFDGRIEKARLYNVRAPSLRSSVYQKCTFLKESAQAIEQRLRKIDHTAIHPHLLDMKIGQGKYQQGFFPKLQADVLASGPTSNKWSNRTCSTQRRVERHRQQNEHLVLDNELKEKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDSAFRPHTHISIAFKCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	XM_005164711.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164711.3

ORF Insert Sequence:

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGC TTTATACCAG 
TGGCTAGAGG CTGATCGGCA GGGCAAGGAT CCAGAGGCCA CAGCAGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_005164711.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164768.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa09340
Clone ID Related Accession (Same CDS sequence)	XM_005164711.3 , XM_005164711.4
Accession Version	XM_005164711.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3978bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164711.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGC TTTATACCAG 
TGGCTAGAGG CTGATCGGCA GGGCAAGGAT CCAGAGGCCA CAGCAGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164768.1
CDS	246..4223
Translation	MSATMSGSGSAPCRFAHYFIVCGLDDETGLEPDALAALYQWLEADRQGKDPEATAAGENFDQSPLKRTFKSKVLAHYPENIECNPFDQDAVNMLCMPKGLSFRTQRDSLAPHFHSFLITREDGSRTYGFVHTFYEEVTSPQICSAMQTLHQMHQAEHNTSAQNCTSSSSSSSSSSSSSSMDSLSSSLDDVDSPSAHGGRRTCEGYDSIRDTLYVSKAMCLIAPMPFMHACKRFLAQMHRAVSCSPPPPLPLESYIYNVLYEVPLPASGRSLKFHGVYEPILCQRPGVGELPLADFPLADAFRLLGVENLVQLFTCVLLEMQILLYSQDYQRLMVVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHCIELPEDFPQFPNRSEFIQELSEVLLSFGQSPEGGSSSPEPAVMSLQTSVLEKELKSTSLRELVDDKTNGNLGGEALDVLELLQGNPTLERLQALAKRTGVKVARLEALAAGQKGDEGVGGRSPVEEEELRNAKLNVQLREVFAARFATMFADYESFVIQNSPDLESWLTNREQMHNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKEPLLRVFDGRIEKARLYNVRAPSLRSSVYQKCTFLKESAQAIEQRLRKIDHTAIHPHLLDMKIGQGKYQQGFFPKLQADVLASGPTSNKWSNRTCSTQRRVERHRQQNEHLVLDNELKEKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDSAFRPHTHISIAFKCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	XM_005164711.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164711.4

ORF Insert Sequence:

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGC TTTATACCAG 
TGGCTAGAGG CTGATCGGCA GGGCAAGGAT CCAGAGGCCA CAGCAGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_005164712.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164769.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa09341
Clone ID Related Accession (Same CDS sequence)	XM_005164712.4 , XM_005164712.3
Accession Version	XM_005164712.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005164712.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164769.1
CDS	246..4163
Translation	MSATMSGSGSAPCRFAHYFIVCGLDDETGLEPDALAGENFDQSPLKRTFKSKVLAHYPENIECNPFDQDAVNMLCMPKGLSFRTQRDSLAPHFHSFLITREDGSRTYGFVHTFYEEVTSPQICSAMQTLHQMHQAEHNTSAQNCTSSSSSSSSSSSSSSMDSLSSSLDDVDSPSAHGGRRTCEGYDSIRDTLYVSKAMCLIAPMPFMHACKRFLAQMHRAVSCSPPPPLPLESYIYNVLYEVPLPASGRSLKFHGVYEPILCQRPGVGELPLADFPLADAFRLLGVENLVQLFTCVLLEMQILLYSQDYQRLMVVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHCIELPEDFPQFPNRSEFIQELSEVLLSFGQSPEGGSSSPEPAVMSLQTSVLEKELKSTSLRELVDDKTNGNLGGEALDVLELLQGNPTLERLQALAKRTGVKVARLEALAAGQKGDEGVGGRSPVEEEELRNAKLNVQLREVFAARFATMFADYESFVIQNSPDLESWLTNREQMHNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKEPLLRVFDGRIEKARLYNVRAPSLRSSVYQKCTFLKESAQAIEQRLRKIDHTAIHPHLLDMKIGQGKYQQGFFPKLQADVLASGPTSNKWSNRTCSTQRRVERHRQQNEHLVLDNELKEKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDSAFRPHTHISIAFKCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	XM_005164712.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164712.4

ORF Insert Sequence:

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_005164712.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005164769.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.

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CloneID	ODa09341
Clone ID Related Accession (Same CDS sequence)	XM_005164712.4 , XM_005164712.3
Accession Version	XM_005164712.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3918bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 5B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005164712.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005164769.1
CDS	283..4200
Translation	MSATMSGSGSAPCRFAHYFIVCGLDDETGLEPDALAGENFDQSPLKRTFKSKVLAHYPENIECNPFDQDAVNMLCMPKGLSFRTQRDSLAPHFHSFLITREDGSRTYGFVHTFYEEVTSPQICSAMQTLHQMHQAEHNTSAQNCTSSSSSSSSSSSSSSMDSLSSSLDDVDSPSAHGGRRTCEGYDSIRDTLYVSKAMCLIAPMPFMHACKRFLAQMHRAVSCSPPPPLPLESYIYNVLYEVPLPASGRSLKFHGVYEPILCQRPGVGELPLADFPLADAFRLLGVENLVQLFTCVLLEMQILLYSQDYQRLMVVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHCIELPEDFPQFPNRSEFIQELSEVLLSFGQSPEGGSSSPEPAVMSLQTSVLEKELKSTSLRELVDDKTNGNLGGEALDVLELLQGNPTLERLQALAKRTGVKVARLEALAAGQKGDEGVGGRSPVEEEELRNAKLNVQLREVFAARFATMFADYESFVIQNSPDLESWLTNREQMHNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKEPLLRVFDGRIEKARLYNVRAPSLRSSVYQKCTFLKESAQAIEQRLRKIDHTAIHPHLLDMKIGQGKYQQGFFPKLQADVLASGPTSNKWSNRTCSTQRRVERHRQQNEHLVLDNELKEKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDSAFRPHTHISIAFKCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	XM_005164712.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005164712.3

ORF Insert Sequence:

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TCTGCCTTTA GGCCTCACAC GCACATCTCC ATCGCCTTCA AGTGCCAGAA TCTGGGCAAG 
CTGACCACTG TTCAGCTGGG TCATGATAAC TCCGGCTTGC TGGCCAAGTG GCTGGTTGAT 
TGCGTCATGG TCCGCAATGA GATCACCGGA CACACATACA AGTTTCCCTG TGGCCGGTGG 
CTGGGAAAGG GTGTGGATGA TGGCAGTCTG GAGCGAGTGC TGATTGGAGA GTTTGTGGTG 
CCTTGCGGCG ATGATGAAGG AGGTCGAGGG TCTAAAACAC CACCGCTCCA GAGGTCGCCC 
TCCCAGATCC GGAGAATCAG CATCACTTCA CTCACCGGAA AAGGAAACAA ACCAACAACC 
GTTCAGATCC AGGAATCTAT CGGTGAAGCA GTCAACAATA TAATTAAACA TTTCCACAAA 
CCAGAGAAAG AGCGGGGCAG TCTCACGGTT CTGCTGTGTG GAGAAGGTGG TCTTGTTTGG 
GCGCTGGAGC AGTTTTTCCA CCATGGGTTT CGCTCTGCTC GAATCTTCCA GAAAAATGTC 
TTTGTTTGGG ATTTCTTTGA GAGAATAGTA GCGTTTATGG AGAACAGAGA TCAAACTGGA 
GACCTGCAAG AATCCCCCGA GCCTCTCAGC CTGAATAGCG AATCACTCTG TCGATACGTC 
AACGCCATCA ACAACATGCC ACGCAACATC GGCAAAGACG GCAAATTCCA GCTGCTAGTT 
TGCCTCGGCG CGAGAGACCG ATTATTACCC CAGTGGCTCC CTCTACTGGC TGAAAGCCCG 
GTCATCACGC GTATGTACGA AGAAAACGCT TTACTTCGAG ACAAACTCAC CGTAAGCTCT 
CTCCTCGGCG TCTTAGAAAC GCTACATGAT TTCCCCATTA CCCTGGAGAC TTCGCTTACA 
AAAAGCGTCG AGCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_009300252.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298527.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa39770
Clone ID Related Accession (Same CDS sequence)	XM_009300252.3 , XM_009300252.2
Accession Version	XM_009300252.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DENN domain-containing protein 5B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009300252.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TGCCAGAATC TGGGCAAGCT GACCACTGTT CAGCTGGGTC ATGATAACTC CGGCTTGCTG 
GCCAAGTGGC TGGTTGATTG CGTCATGGTC CGCAATGAGA TCACCGGACA CACATACAAG 
TTTCCCTGTG GCCGGTGGCT GGGAAAGGGT GTGGATGATG GCAGTCTGGA GCGAGTGCTG 
ATTGGAGAGT TTGTGGTGCC TTGCGGCGAT GATGAAGGAG GTCGAGGGTC TAAAACACCA 
CCGCTCCAGA GGTCGCCCTC CCAGATCCGG AGAATCAGCA TCACTTCACT CACCGGAAAA 
GGAAACAAAC CAACAACCGT TCAGATCCAG GAATCTATCG GTGAAGCAGT CAACAATATA 
ATTAAACATT TCCACAAACC AGAGAAAGAG CGGGGCAGTC TCACGGTTCT GCTGTGTGGA 
GAAGGTGGTC TTGTTTGGGC GCTGGAGCAG TTTTTCCACC ATGGGTTTCG CTCTGCTCGA 
ATCTTCCAGA AAAATGTCTT TGTTTGGGAT TTCTTTGAGA GAATAGTAGC GTTTATGGAG 
AACAGAGATC AAACTGGAGA CCTGCAAGAA TCCCCCGAGC CTCTCAGCCT GAATAGCGAA 
TCACTCTGTC GATACGTCAA CGCCATCAAC AACATGCCAC GCAACATCGG CAAAGACGGC 
AAATTCCAGC TGCTAGTTTG CCTCGGCGCG AGAGACCGAT TATTACCCCA GTGGCTCCCT 
CTACTGGCTG AAAGCCCGGT CATCACGCGT ATGTACGAAG AAAACGCTTT ACTTCGAGAC 
AAACTCACCG TAAGCTCTCT CCTCGGCGTC TTAGAAACGC TACATGATTT CCCCATTACC 
CTGGAGACTT CGCTTACAAA AAGCGTCGAG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298527.1
CDS	245..4120
Translation	MSATMSGSGSAPCRFAHYFIVCGLDDETGLEPDALAGENFDQSPLKRTFKSKVLAHYPENIECNPFDQDAVNMLCMPKGLSFRTQRDSLAPHFHSFLITREDGSRTYGFVHTFYEEVTSPQICSAMQTLHQMHQAEHNTSAQNCTSSSSSSSSSSSSSSMDSLSSSLDDVDSPSAHGGRRTCEGYDSIRDTLYVSKAMCLIAPMPFMHACKRFLAQMHRAVSCSPPPPLPLESYIYNVLYEVPLPASGRSLKFHGVYEPILCQRPGVGELPLADFPLADAFRLLGVENLVQLFTCVLLEMQILLYSQDYQRLMVVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHCIELPEDFPQFPNRSEFIQELSEVLLSFGQSPEGGSSSPEPAVMSLQTSVLEKELKSTSLRELVDDKTNGNLGGEALDVLELLQGNPTLERLQALAKRTGVKVARLEALAAGQKGDEGVGGRSPVEEEELRNAKLNVQLREVFAARFATMFADYESFVIQNSPDLESWLTNREQMHNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKEPLLRVFDGRIEKARLYNVRAPSLRSSVYQKCTFLKESAQAIEQRLRKIDHTAIHPHLLDMKIGQGKYQQGFFPKLQADVLASGPTSNKWSNRTCSTQRRVERHRQQNEHLVLDNELKEKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	XM_009300252.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300252.3

ORF Insert Sequence:

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TGCCAGAATC TGGGCAAGCT GACCACTGTT CAGCTGGGTC ATGATAACTC CGGCTTGCTG 
GCCAAGTGGC TGGTTGATTG CGTCATGGTC CGCAATGAGA TCACCGGACA CACATACAAG 
TTTCCCTGTG GCCGGTGGCT GGGAAAGGGT GTGGATGATG GCAGTCTGGA GCGAGTGCTG 
ATTGGAGAGT TTGTGGTGCC TTGCGGCGAT GATGAAGGAG GTCGAGGGTC TAAAACACCA 
CCGCTCCAGA GGTCGCCCTC CCAGATCCGG AGAATCAGCA TCACTTCACT CACCGGAAAA 
GGAAACAAAC CAACAACCGT TCAGATCCAG GAATCTATCG GTGAAGCAGT CAACAATATA 
ATTAAACATT TCCACAAACC AGAGAAAGAG CGGGGCAGTC TCACGGTTCT GCTGTGTGGA 
GAAGGTGGTC TTGTTTGGGC GCTGGAGCAG TTTTTCCACC ATGGGTTTCG CTCTGCTCGA 
ATCTTCCAGA AAAATGTCTT TGTTTGGGAT TTCTTTGAGA GAATAGTAGC GTTTATGGAG 
AACAGAGATC AAACTGGAGA CCTGCAAGAA TCCCCCGAGC CTCTCAGCCT GAATAGCGAA 
TCACTCTGTC GATACGTCAA CGCCATCAAC AACATGCCAC GCAACATCGG CAAAGACGGC 
AAATTCCAGC TGCTAGTTTG CCTCGGCGCG AGAGACCGAT TATTACCCCA GTGGCTCCCT 
CTACTGGCTG AAAGCCCGGT CATCACGCGT ATGTACGAAG AAAACGCTTT ACTTCGAGAC 
AAACTCACCG TAAGCTCTCT CCTCGGCGTC TTAGAAACGC TACATGATTT CCCCATTACC 
CTGGAGACTT CGCTTACAAA AAGCGTCGAG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dennd5b ( XM_009300252.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009298527.1 Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39770
Clone ID Related Accession (Same CDS sequence)	XM_009300252.3 , XM_009300252.2
Accession Version	XM_009300252.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3876bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DENN domain-containing protein 5B isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007115.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009300252.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TGCCAGAATC TGGGCAAGCT GACCACTGTT CAGCTGGGTC ATGATAACTC CGGCTTGCTG 
GCCAAGTGGC TGGTTGATTG CGTCATGGTC CGCAATGAGA TCACCGGACA CACATACAAG 
TTTCCCTGTG GCCGGTGGCT GGGAAAGGGT GTGGATGATG GCAGTCTGGA GCGAGTGCTG 
ATTGGAGAGT TTGTGGTGCC TTGCGGCGAT GATGAAGGAG GTCGAGGGTC TAAAACACCA 
CCGCTCCAGA GGTCGCCCTC CCAGATCCGG AGAATCAGCA TCACTTCACT CACCGGAAAA 
GGAAACAAAC CAACAACCGT TCAGATCCAG GAATCTATCG GTGAAGCAGT CAACAATATA 
ATTAAACATT TCCACAAACC AGAGAAAGAG CGGGGCAGTC TCACGGTTCT GCTGTGTGGA 
GAAGGTGGTC TTGTTTGGGC GCTGGAGCAG TTTTTCCACC ATGGGTTTCG CTCTGCTCGA 
ATCTTCCAGA AAAATGTCTT TGTTTGGGAT TTCTTTGAGA GAATAGTAGC GTTTATGGAG 
AACAGAGATC AAACTGGAGA CCTGCAAGAA TCCCCCGAGC CTCTCAGCCT GAATAGCGAA 
TCACTCTGTC GATACGTCAA CGCCATCAAC AACATGCCAC GCAACATCGG CAAAGACGGC 
AAATTCCAGC TGCTAGTTTG CCTCGGCGCG AGAGACCGAT TATTACCCCA GTGGCTCCCT 
CTACTGGCTG AAAGCCCGGT CATCACGCGT ATGTACGAAG AAAACGCTTT ACTTCGAGAC 
AAACTCACCG TAAGCTCTCT CCTCGGCGTC TTAGAAACGC TACATGATTT CCCCATTACC 
CTGGAGACTT CGCTTACAAA AAGCGTCGAG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009298527.1
CDS	283..4158
Translation	MSATMSGSGSAPCRFAHYFIVCGLDDETGLEPDALAGENFDQSPLKRTFKSKVLAHYPENIECNPFDQDAVNMLCMPKGLSFRTQRDSLAPHFHSFLITREDGSRTYGFVHTFYEEVTSPQICSAMQTLHQMHQAEHNTSAQNCTSSSSSSSSSSSSSSMDSLSSSLDDVDSPSAHGGRRTCEGYDSIRDTLYVSKAMCLIAPMPFMHACKRFLAQMHRAVSCSPPPPLPLESYIYNVLYEVPLPASGRSLKFHGVYEPILCQRPGVGELPLADFPLADAFRLLGVENLVQLFTCVLLEMQILLYSQDYQRLMVVAEGITTLLFPFQWQHVYVPILPASLLHFLDAPVPYLMGLQSKEGTDRSKLELPQEANLCFVDIDNHCIELPEDFPQFPNRSEFIQELSEVLLSFGQSPEGGSSSPEPAVMSLQTSVLEKELKSTSLRELVDDKTNGNLGGEALDVLELLQGNPTLERLQALAKRTGVKVARLEALAAGQKGDEGVGGRSPVEEEELRNAKLNVQLREVFAARFATMFADYESFVIQNSPDLESWLTNREQMHNFDKASFLSDQPEPYLPFLSHFIETQMFATFIDNKIMSQWEEKEPLLRVFDGRIEKARLYNVRAPSLRSSVYQKCTFLKESAQAIEQRLRKIDHTAIHPHLLDMKIGQGKYQQGFFPKLQADVLASGPTSNKWSNRTCSTQRRVERHRQQNEHLVLDNELKEKYMQEARNLGKNLRQPKLSDLSPAVIAQTNWKFVEGLLKECKMKTKRMLVEKMGREAVELGHGEANITGLEENTLIASLCDLLERIWSHGLQVKQGKSALWSHLLHYQAREEKNEQQLESPVSNGQERRKTESSVGLPGLRVSVIQDMRHIQNMAEIKTDVGRARAWIRLSLEKKLLSQHLKQLLSNQALTKKLYKRYAFLRCEEEKEQFLFHLLSLNAVDYFCFTSVFTTIMIPYRAVIIPIKKLSNAMTTSNPWLCVSGELGDSGILQITKNILEMTFDCQNLGKLTTVQLGHDNSGLLAKWLVDCVMVRNEITGHTYKFPCGRWLGKGVDDGSLERVLIGEFVVPCGDDEGGRGSKTPPLQRSPSQIRRISITSLTGKGNKPTTVQIQESIGEAVNNIIKHFHKPEKERGSLTVLLCGEGGLVWALEQFFHHGFRSARIFQKNVFVWDFFERIVAFMENRDQTGDLQESPEPLSLNSESLCRYVNAINNMPRNIGKDGKFQLLVCLGARDRLLPQWLPLLAESPVITRMYEENALLRDKLTVSSLLGVLETLHDFPITLETSLTKSVEL

Target ORF information:

RefSeq Version	XM_009300252.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio DENN/MADD domain containing 5B (dennd5b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009300252.2

ORF Insert Sequence:

ATGAGCGCGA CTATGAGCGG CTCTGGATCT GCGCCCTGCC GCTTTGCACA TTACTTCATA 
GTATGCGGAT TAGACGACGA AACGGGACTT GAACCCGATG CTTTGGCAGG GGAAAACTTT 
GATCAGAGTC CCCTAAAAAG GACCTTCAAG TCTAAAGTGT TGGCTCACTA CCCGGAGAAC 
ATTGAGTGCA ACCCATTTGA CCAGGATGCT GTTAACATGC TCTGTATGCC TAAAGGCCTG 
TCATTCAGGA CGCAGCGGGA CAGTCTGGCT CCGCACTTCC ACTCGTTCCT CATCACCCGA 
GAGGACGGCT CACGGACTTA CGGCTTCGTC CATACCTTCT ATGAGGAGGT GACGAGTCCT 
CAGATCTGCT CCGCCATGCA GACGCTCCAT CAGATGCACC AGGCTGAACA CAACACATCT 
GCACAGAACT GTACTTCATC CTCCTCATCA TCCTCCTCCT CCTCTTCCTC CTCTAGCATG 
GACTCTTTAT CCAGCAGTCT GGATGACGTT GACTCTCCAT CCGCTCATGG AGGCAGAAGA 
ACCTGTGAAG GCTACGACTC GATCCGCGAC ACTCTTTATG TGTCCAAAGC CATGTGTTTG 
ATTGCACCGA TGCCCTTCAT GCACGCTTGT AAACGTTTTT TGGCTCAAAT GCACCGGGCT 
GTGAGCTGTA GTCCTCCGCC GCCACTTCCT CTAGAGAGTT ACATCTATAA CGTGTTGTAT 
GAGGTGCCGC TGCCGGCGTC TGGACGCTCG CTCAAGTTTC ATGGAGTTTA TGAACCAATC 
CTGTGTCAGC GGCCGGGAGT CGGAGAGCTG CCATTGGCTG ATTTTCCACT GGCCGACGCC 
TTCCGGTTAC TGGGGGTGGA GAATCTGGTG CAGCTGTTCA CCTGTGTCCT GCTGGAAATG 
CAGATTCTGC TGTACTCACA GGACTACCAG CGGCTCATGG TGGTTGCGGA GGGCATCACA 
ACATTGCTCT TCCCTTTCCA GTGGCAGCAC GTGTATGTCC CGATTCTCCC TGCTTCACTC 
CTGCACTTTC TAGACGCCCC TGTCCCATAC CTCATGGGGC TTCAATCTAA AGAGGGCACT 
GACCGCTCCA AACTAGAGCT GCCTCAAGAG GCAAACCTGT GTTTCGTAGA CATCGACAAC 
CACTGCATCG AGCTTCCGGA AGACTTCCCT CAGTTTCCCA ACAGGAGTGA GTTCATCCAG 
GAGCTGAGCG AGGTGCTGCT CAGTTTTGGC CAGTCTCCCG AAGGGGGTAG CAGCTCTCCT 
GAACCGGCCG TGATGAGCTT GCAGACCTCT GTGCTGGAGA AGGAGCTCAA GAGCACTTCG 
TTGAGAGAGT TGGTGGACGA TAAGACGAAC GGCAACCTCG GTGGAGAAGC GCTGGATGTG 
CTGGAGCTCC TGCAGGGCAA CCCGACGCTG GAGCGCCTGC AGGCTTTGGC CAAGAGGACT 
GGGGTGAAAG TGGCCCGTCT GGAGGCGCTG GCAGCGGGAC AGAAGGGGGA TGAAGGTGTT 
GGAGGAAGGT CTCCTGTTGA GGAAGAGGAG CTGAGGAATG CTAAACTGAA CGTTCAGCTG 
AGGGAGGTGT TCGCCGCACG CTTCGCAACG ATGTTTGCAG ACTACGAGTC GTTTGTGATT 
CAGAACTCGC CAGATTTGGA GTCTTGGCTG ACCAACAGAG AGCAAATGCA CAACTTTGAC 
AAGGCCTCGT TTCTTTCGGA CCAGCCGGAG CCATACCTGC CCTTTCTGTC TCATTTCATC 
GAAACCCAAA TGTTTGCCAC CTTCATCGAC AACAAGATCA TGTCTCAGTG GGAGGAAAAA 
GAGCCTCTCC TTCGGGTCTT TGATGGACGC ATTGAGAAAG CCAGACTCTA CAATGTCCGT 
GCACCCAGTT TACGCTCATC TGTTTACCAG AAATGCACTT TCCTCAAAGA ATCAGCCCAG 
GCTATTGAGC AGCGGCTGAG AAAGATCGAC CACACGGCCA TCCATCCTCA CCTGCTGGAT 
ATGAAGATTG GCCAGGGAAA ATACCAGCAG GGCTTTTTCC CCAAACTGCA GGCAGATGTG 
TTAGCCTCTG GGCCCACCAG CAACAAGTGG TCAAATCGCA CATGTTCGAC TCAGCGCAGA 
GTGGAACGAC ACAGGCAGCA GAATGAACAT CTGGTGCTGG ACAATGAACT CAAAGAGAAG 
TACATGCAGG AGGCTCGTAA CCTGGGGAAG AACCTGCGAC AACCCAAATT ATCAGACTTG 
TCCCCTGCCG TCATCGCACA GACCAACTGG AAGTTTGTGG AGGGTTTACT GAAGGAATGC 
AAGATGAAGA CCAAGCGCAT GTTGGTGGAG AAAATGGGGA GAGAAGCTGT CGAACTGGGT 
CACGGAGAGG CAAACATCAC TGGTCTGGAA GAAAACACAC TCATAGCCAG TCTGTGTGAC 
CTGCTGGAGA GAATATGGAG CCACGGGCTT CAGGTTAAAC AGGGAAAGTC TGCACTTTGG 
TCTCATTTAT TGCATTACCA GGCGAGAGAA GAGAAAAACG AACAGCAGTT GGAGTCTCCG 
GTGTCTAATG GCCAGGAAAG ACGGAAAACC GAGTCCAGCG TTGGTCTTCC TGGATTACGT 
GTGTCCGTCA TACAGGATAT GAGACACATC CAGAACATGG CAGAGATTAA GACTGATGTA 
GGTCGAGCAC GTGCCTGGAT CCGTCTGTCT CTGGAGAAAA AACTGCTTTC CCAGCACCTC 
AAACAGCTGC TGTCAAACCA GGCCTTAACC AAGAAGCTCT ACAAACGTTA CGCTTTTCTC 
CGCTGTGAAG AGGAGAAAGA GCAGTTTCTC TTTCATCTCC TGAGCCTGAA TGCTGTGGAC 
TACTTCTGCT TTACCAGCGT CTTCACTACT ATAATGATCC CATACCGGGC TGTGATCATC 
CCCATCAAGA AGCTGAGTAA CGCCATGACC ACGTCCAACC CCTGGCTGTG TGTGTCGGGC 
GAGCTGGGCG ACTCCGGCAT CCTGCAGATC ACCAAAAACA TACTGGAGAT GACATTCGAC 
TGCCAGAATC TGGGCAAGCT GACCACTGTT CAGCTGGGTC ATGATAACTC CGGCTTGCTG 
GCCAAGTGGC TGGTTGATTG CGTCATGGTC CGCAATGAGA TCACCGGACA CACATACAAG 
TTTCCCTGTG GCCGGTGGCT GGGAAAGGGT GTGGATGATG GCAGTCTGGA GCGAGTGCTG 
ATTGGAGAGT TTGTGGTGCC TTGCGGCGAT GATGAAGGAG GTCGAGGGTC TAAAACACCA 
CCGCTCCAGA GGTCGCCCTC CCAGATCCGG AGAATCAGCA TCACTTCACT CACCGGAAAA 
GGAAACAAAC CAACAACCGT TCAGATCCAG GAATCTATCG GTGAAGCAGT CAACAATATA 
ATTAAACATT TCCACAAACC AGAGAAAGAG CGGGGCAGTC TCACGGTTCT GCTGTGTGGA 
GAAGGTGGTC TTGTTTGGGC GCTGGAGCAG TTTTTCCACC ATGGGTTTCG CTCTGCTCGA 
ATCTTCCAGA AAAATGTCTT TGTTTGGGAT TTCTTTGAGA GAATAGTAGC GTTTATGGAG 
AACAGAGATC AAACTGGAGA CCTGCAAGAA TCCCCCGAGC CTCTCAGCCT GAATAGCGAA 
TCACTCTGTC GATACGTCAA CGCCATCAAC AACATGCCAC GCAACATCGG CAAAGACGGC 
AAATTCCAGC TGCTAGTTTG CCTCGGCGCG AGAGACCGAT TATTACCCCA GTGGCTCCCT 
CTACTGGCTG AAAGCCCGGT CATCACGCGT ATGTACGAAG AAAACGCTTT ACTTCGAGAC 
AAACTCACCG TAAGCTCTCT CCTCGGCGTC TTAGAAACGC TACATGATTT CCCCATTACC 
CTGGAGACTT CGCTTACAAA AAGCGTCGAG CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

A gene expression screen in zebrafish embryogenesis.
Genome research11(12)1979-87(2001 Dec)
Kudoh T,Tsang M,Hukriede NA,Chen X,Dedekian M,Clarke CJ,Kiang A,Schultz S,Epstein JA,Toyama R,Dawid IB

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dennd5b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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