actn4 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	actn4
Entrez Gene ID	322221
Full Name	actinin, alpha 4
Synonyms	wu:fb53f05,zgc:63508
General protein information	Preferred Names actinin, alpha 4 Names alpha-actinin-4
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021481334.1	XP_021337009.1	alpha-actinin-4 isoform X1
XM_021481337.1	XP_021337012.1	alpha-actinin-4 isoform X4
NM_199586.1	NP_955880.1	alpha-actinin-4
XM_021481335.1	XP_021337010.1	alpha-actinin-4 isoform X2
XM_021481338.1	XP_021337013.1	alpha-actinin-4 isoform X5
XM_021481336.1	XP_021337011.1	alpha-actinin-4 isoform X3
XM_021481339.1	XP_021337014.1	alpha-actinin-4 isoform X6

Pathways from BioSystems

Homologies

	ACTN4	XP_001083825.1
Danio rerio (zebrafish)	actn4	NP_955880.1
Pan troglodytes (chimpanzee)	ACTN4	XP_003954421.1
Rattus norvegicus (Norway rat)	Actn4	NP_113863.2
Homo sapiens (human)	ACTN4	NP_004915.2
Bos taurus (cattle)	ACTN4	NP_001091521.1
Schizosaccharomyces pombe (fission yeast)	ain1	NP_594295.1
Canis lupus familiaris (dog)	ACTN4	XP_541640.2
Mus musculus (house mouse)	Actn4	NP_068695.1
Gallus gallus (chicken)	ACTN4	NP_990457.1
Magnaporthe oryzae (rice blast fungus)	MGG_06475	XP_003717088.1
Neurospora crassa	NCU06429	XP_957320.1
Xenopus tropicalis (tropical clawed frog)	actn4	NP_001006810.1

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.
Tseng LC, Zhang C, Cheng CM, Xu H, Hsu CH, Jiang YJ
PloS one9(4)e93394(2014)

?-Actinin-2 deficiency results in sarcomeric defects in zebrafish that cannot be rescued by ?-actinin-3 revealing functional differences between sarcomeric isoforms.
Gupta V, Discenza M, Guyon JR, Kunkel LM, Beggs AH
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(5)1892-908(2012 May)

Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung.
Zheng W, Wang Z, Collins JE, Andrews RM, Stemple D, Gong Z
PloS one6(8)e24019(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following actn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa04952	NM_199586.1 Latest version!	Danio rerio actinin, alpha 4 (actn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa148921	XM_021481335.1 Latest version!	Danio rerio actinin, alpha 4 (actn4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa148921	XM_021481334.1 Latest version!	Danio rerio actinin, alpha 4 (actn4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa148922	XM_021481336.1 Latest version!	Danio rerio actinin, alpha 4 (actn4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa148923	XM_021481337.1 Latest version!	Danio rerio actinin, alpha 4 (actn4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa148924	XM_021481338.1 Latest version!	Danio rerio actinin, alpha 4 (actn4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa148925	XM_021481339.1 Latest version!	Danio rerio actinin, alpha 4 (actn4), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actn4 ( NM_199586.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_955880.1 Danio rerio actinin, alpha 4 (actn4), mRNA.

Price & Availability↑

CloneID	ODa04952
Clone ID Related Accession (Same CDS sequence)	NM_199586.1
Accession Version	NM_199586.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054901.1. On Jul 6, 2005 this sequence version replaced XM_690966.1. ##Evidence-Data-START## Transcript exon combination :: BC054901.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA 
TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC 
TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCGGACCA GGTCATCGCC 
TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG 
CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT 
GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_955880.1
CDS	156..2861
Translation	MVDYHAVNNQGQYSAGGPPTYMEQENDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIDYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINGAWHKLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEGWTDGKEAMLTQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIANYNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPWIQNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIFDNKYTAYTMEHLRVGWEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKDHSGLLQSEEFKACLISLGYDVENDKQGDAEFARIMGIVDPNNSGAVTFQAFIDFMSRETTDTDTADQVIASFKILAGDKNYITAEELRRELPPDQAEYCIARMAPYSGPDAVAGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_199586.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 4 (actn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_199586.1

ORF Insert Sequence:

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA 
TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC 
TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCGGACCA GGTCATCGCC 
TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG 
CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT 
GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_021481334.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337009.1 Danio rerio actinin, alpha 4 (actn4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148921
Clone ID Related Accession (Same CDS sequence)	XM_021481334.1 , XM_021481335.1
Accession Version	XM_021481334.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG 
ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT 
GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC 
CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC 
ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG 
CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG 
CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG 
AGCGACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337009.1
CDS	184..2955
Translation	MVDYHAVNNQGQYSAGGPPTYMEQENDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIDYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINGAWHKLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEGWTDGKEAMLTQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIANYNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPWIQNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIFDNKYTAYTMEHLRVGWEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKDHSGLLQSEEFKACLISLGYDVENDKQKRTGIMDSDDFRALLISTGNSLGDAEFARIMGIVDPNNSGAVTFQAFIDFMSRETTDTDTADQVIASFKILAGDKNYITAEELRRELPPDQAEYCIARMAPYSGPDAVAGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_021481334.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 4 (actn4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481334.1

ORF Insert Sequence:

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG 
ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT 
GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC 
CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC 
ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG 
CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG 
CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG 
AGCGACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_021481335.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337010.1 Danio rerio actinin, alpha 4 (actn4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa148921
Clone ID Related Accession (Same CDS sequence)	XM_021481334.1 , XM_021481335.1
Accession Version	XM_021481335.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG 
ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT 
GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC 
CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC 
ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG 
CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG 
CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG 
AGCGACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337010.1
CDS	184..2955
Translation	MVDYHAVNNQGQYSAGGPPTYMEQENDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIDYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINGAWHKLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEGWTDGKEAMLTQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIANYNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPWIQNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIFDNKYTAYTMEHLRVGWEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKDHSGLLQSEEFKACLISLGYDVENDKQKRTGIMDSDDFRALLISTGNSLGDAEFARIMGIVDPNNSGAVTFQAFIDFMSRETTDTDTADQVIASFKILAGDKNYITAEELRRELPPDQAEYCIARMAPYSGPDAVAGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_021481335.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 4 (actn4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481335.1

ORF Insert Sequence:

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG 
ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT 
GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC 
CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC 
ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG 
CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG 
CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG 
AGCGACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_021481336.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337011.1 Danio rerio actinin, alpha 4 (actn4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa148922
Clone ID Related Accession (Same CDS sequence)	XM_021481336.1
Accession Version	XM_021481336.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2772bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA 
CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA 
GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG 
ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT 
GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC 
CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC 
ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG 
CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG 
CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG 
AGCGACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337011.1
CDS	184..2955
Translation	MVDYHAVNNQGQYSAGGPPTYMEQENDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIDYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINGAWHKLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEGWTDGKEAMLTQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIANYNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPWIQNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIFDNKYTAYTMEHLRVGWEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKDHSGLLQSEEFKACLISLGYDVENDKQKRTGIMDSDDFRALLISTGNSLGDAEFARIMGIVDPNNSGAVTFQAFIDFMSRETTDTDTADQVIASFKILAGDKNYITAEELRRELPPDQAEYCIARMAPYSGPDAVAGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_021481336.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 4 (actn4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481336.1

ORF Insert Sequence:

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA 
CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA 
GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGAA GCGCACGGGG 
ATAATGGACA GCGATGATTT CCGTGCTCTG CTCATTTCCA CTGGGAACAG CCTGGGTGAT 
GCTGAATTCG CTCGTATCAT GGGCATTGTT GACCCCAATA ACAGTGGAGC AGTTACCTTC 
CAGGCCTTCA TCGACTTCAT GTCAAGGGAA ACGACCGACA CAGACACTGC AGACCAGGTC 
ATCGCCTCAT TTAAGATCCT GGCTGGAGAC AAGAACTACA TCACAGCCGA GGAGCTGAGG 
CGTGAGCTTC CTCCAGATCA GGCAGAGTAC TGCATCGCCC GAATGGCGCC GTACTCTGGG 
CCGGATGCAG TGGCTGGCGC TCTTGACTAC ATGTCCTTCT CCACCGCCCT GTACGGGGAG 
AGCGACCTCT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_021481337.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337012.1 Danio rerio actinin, alpha 4 (actn4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa148923
Clone ID Related Accession (Same CDS sequence)	XM_021481337.1
Accession Version	XM_021481337.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2706bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA 
CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA 
GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA 
TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC 
TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCAGACCA GGTCATCGCC 
TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG 
CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT 
GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337012.1
CDS	184..2889
Translation	MVDYHAVNNQGQYSAGGPPTYMEQENDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIDYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINGAWHKLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEGWTDGKEAMLTQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIANYNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPWIQNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIFDNKYTAYTMEHLRVGWEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKDHSGLLQSEEFKACLISLGYDVENDKQGDAEFARIMGIVDPNNSGAVTFQAFIDFMSRETTDTDTADQVIASFKILAGDKNYITAEELRRELPPDQAEYCIARMAPYSGPDAVAGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_021481337.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 4 (actn4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481337.1

ORF Insert Sequence:

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA 
CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA 
GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGGAT CACAGTGGTC TCCTGCAGTC TGAGGAGTTC 
AAGGCTTGCT TGATCAGTCT GGGTTACGAC GTTGAAAATG ACAAGCAGGG TGATGCTGAA 
TTCGCTCGTA TCATGGGCAT TGTTGACCCC AATAACAGTG GAGCAGTTAC CTTCCAGGCC 
TTCATCGACT TCATGTCAAG GGAAACGACC GACACAGACA CTGCAGACCA GGTCATCGCC 
TCATTTAAGA TCCTGGCTGG AGACAAGAAC TACATCACAG CCGAGGAGCT GAGGCGTGAG 
CTTCCTCCAG ATCAGGCAGA GTACTGCATC GCCCGAATGG CGCCGTACTC TGGGCCGGAT 
GCAGTGGCTG GCGCTCTTGA CTACATGTCC TTCTCCACCG CCCTGTACGG GGAGAGCGAC 
CTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_021481338.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337013.1 Danio rerio actinin, alpha 4 (actn4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa148924
Clone ID Related Accession (Same CDS sequence)	XM_021481338.1
Accession Version	XM_021481338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC 
CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG 
GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG 
TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG 
GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG 
GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT 
CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337013.1
CDS	184..2874
Translation	MVDYHAVNNQGQYSAGGPPTYMEQENDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIDYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINGAWHKLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEGWTDGKEAMLTQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIANYNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPWIQNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIFDNKYTAYTMEHLRVGWEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKKRTGIMDSDDFRALLISTGNSLGDAEFARIMGIVDPNNSGAVTFQAFIDFMSRETTDTDTADQVIASFKILAGDKNYITAEELRRELPPDQAEYCIARMAPYSGPDAVAGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_021481338.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 4 (actn4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481338.1

ORF Insert Sequence:

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGATATCGT GGGCACTCTG 
CGGCCGGATG AGAAGGCTAT TATGACCTAC GTCTCCTGTT TCTATCACGC CTTCTCAGGT 
GCTCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC 
CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG 
GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG 
TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG 
GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG 
GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT 
CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_021481339.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021337014.1 Danio rerio actinin, alpha 4 (actn4), transcript variant X6, mRNA.

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CloneID	ODa148925
Clone ID Related Accession (Same CDS sequence)	XM_021481339.1
Accession Version	XM_021481339.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2691bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	alpha-actinin-4 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA 
CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA 
GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC 
CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG 
GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG 
TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG 
GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG 
GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT 
CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021337014.1
CDS	184..2874
Translation	MVDYHAVNNQGQYSAGGPPTYMEQENDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIDYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRAPEKTMAEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINGAWHKLEGAEKGYEEWLLNEIRRLERLDHLAEKFRQKAAIHEGWTDGKEAMLTQKDYETASLSEIKALLKKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICEQWDALGSLTQNRRESLERTEKQLESIDELYLEYAKRAAPFNNWMEGAMEDLQDMFIVHNIDEIQGLITAHEQFKSTLPEANKEREAIQAIQAEVQKIANYNGIKLAGNNPYTTITPQSIDKKWEKVQQLVPQRDQALQEELTRQQSNDHLRRQFANQANMIGPWIQNKMEEIGRISIEMNGTLEDQLTHLSQYEQSIIEYKPNIDQLEGDHQLIQEALIFDNKYTAYTMEHLRVGWEQLLTTIARTINEIENQVLTRDAKGISQEQLHEYRTSFNHFDKKRTGIMDSDDFRALLISTGNSLGDAEFARIMGIVDPNNSGAVTFQAFIDFMSRETTDTDTADQVIASFKILAGDKNYITAEELRRELPPDQAEYCIARMAPYSGPDAVAGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_021481339.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio actinin, alpha 4 (actn4), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021481339.1

ORF Insert Sequence:

ATGGTAGATT ATCACGCCGT TAATAATCAA GGCCAGTATT CAGCGGGCGG GCCACCGACC 
TACATGGAGC AAGAGAACGA CTGGGACCGG GACCTGCTGC TGGACCCGGC CTGGGAGAAG 
CAGCAGAGGA AGACTTTCAC GGCCTGGTGC AACTCTCACC TGAGGAAAGC GGGAACGCAG 
ATCGAAAACA TCGAGGAGGA TTTCAGAGAT GGACTCAAAC TAATGCTGCT CCTGGAGGTC 
ATTTCAGGGG AGAGGTTACC AAAACCTGAA AGAGGCAAGA TGAGAGTGCA CAAGATCAAC 
AATGTCAACA AAGCGCTGGA CTTCATCGCC AGCAAAGGAG TCAAACTGGT GTCCATCGGT 
GCAGAAGAAA TTGTGGATGG AAATGCCAAG ATGACTTTGG GAATGATTTG GACCATCATC 
CTCCGCTTCG CCATCCAGGA CATCTCAGTG GAAGAAACCT CCGCTAAAGA AGGCCTGTTG 
CTCTGGTGTC AGAGAAAGAC GGCTCCTTAC AAGAATGTCA ACGTCCAGAA CTTCCATATC 
AGCTGGAAGG ATGGTCTTGC CTTCAATGCT CTGATCCACA GACACAGACC AGAGCTCATC 
GATTACGACA AGCTGAGAAA GGATGACCCA GTGACCAATC TGAACAATGC TTTCGAAGTG 
GCAGAGCGAT ACCTGGACAT CCCCAAGATG TTGGATGCTG AGGACATTGT GAACACAGCA 
CGTCCAGATG AGAAGGCCAT AATGACCTAT GTGTCCAGTT TCTACCATGC CTTTTCTGGA 
GCCCAGAAGG CTGAGACGGC AGCCAATCGT ATCTGCAAGG TTTTGGCAGT CAATCAGGAA 
AACGAGCACC TGATGGAGGA CTATGAGAAA CTGGCCAGTG ATCTGTTGGA GTGGATTCGT 
AGGACAATCC CATGGTTGGA AAACCGCGCT CCAGAGAAAA CCATGGCAGA AATGCAGCAG 
AAACTGGAAG ATTTCCGCGA TTACCGTCGT GTTCACAAAC CTCCCAAAGT TCAGGAGAAG 
TGTCAGCTAG AGATCAACTT CAACACGCTG CAGACCAAAC TCCGTTTGAG CAACCGACCG 
GCCTTCATGC CATCTGAAGG ACGCATGGTG TCGGATATTA ATGGTGCGTG GCACAAACTG 
GAAGGGGCAG AAAAAGGCTA TGAAGAGTGG CTCCTGAATG AGATCCGTCG CTTGGAGAGA 
CTTGACCATC TTGCAGAAAA GTTCCGTCAG AAAGCAGCCA TCCATGAGGG CTGGACAGAC 
GGTAAAGAGG CCATGCTCAC ACAAAAGGAC TATGAAACCG CATCCCTGTC TGAGATTAAA 
GCTCTCCTGA AGAAACACGA GGCCTTCGAG AGCGACCTTG CCGCCCACCA GGACAGAGTG 
GAGCAGATTG CCGCCATCGC ACAGGAGCTC AATGAGCTGG ACTACTACGA CTCCCCGAGC 
GTAAATGCAC GCTGTCAGAA GATCTGCGAG CAGTGGGATG CTCTGGGCTC CCTCACACAG 
AACCGCAGAG AGTCTCTGGA GAGAACAGAG AAGCAGCTGG AGTCTATCGA TGAGCTGTAC 
CTGGAGTACG CCAAGAGAGC GGCACCATTC AACAACTGGA TGGAGGGCGC CATGGAGGAC 
CTGCAGGACA TGTTCATTGT GCACAACATC GATGAGATTC AGGGTCTGAT AACAGCTCAT 
GAGCAGTTCA AATCAACTCT TCCAGAGGCA AATAAAGAGC GGGAAGCTAT CCAAGCCATC 
CAGGCTGAAG TTCAGAAAAT TGCAAATTAC AACGGCATCA AACTGGCTGG AAACAATCCT 
TACACCACCA TCACCCCGCA GAGCATTGAC AAGAAGTGGG AGAAGGTGCA GCAACTGGTT 
CCACAGCGTG ATCAGGCACT ACAGGAGGAA CTCACCCGTC AGCAGTCCAA TGATCATCTC 
AGACGCCAGT TTGCCAACCA GGCCAACATG ATCGGACCAT GGATCCAAAA TAAGATGGAG 
GAGATTGGCC GGATATCCAT TGAGATGAAT GGAACTCTAG AGGATCAGCT GACCCACCTC 
AGCCAGTACG AGCAGAGCAT CATCGAATAC AAGCCCAACA TCGACCAACT GGAGGGAGAC 
CATCAGCTCA TTCAGGAAGC ACTTATCTTT GACAACAAAT ACACCGCTTA CACTATGGAG 
CATCTTCGTG TTGGATGGGA GCAGCTCCTC ACTACAATCG CCCGCACCAT TAACGAGATT 
GAGAACCAGG TTCTGACCCG CGATGCTAAA GGCATTAGCC AGGAACAGCT TCACGAGTAC 
CGCACATCCT TCAATCACTT CGACAAGAAG CGCACGGGGA TAATGGACAG CGATGATTTC 
CGTGCTCTGC TCATTTCCAC TGGGAACAGC CTGGGTGATG CTGAATTCGC TCGTATCATG 
GGCATTGTTG ACCCCAATAA CAGTGGAGCA GTTACCTTCC AGGCCTTCAT CGACTTCATG 
TCAAGGGAAA CGACCGACAC AGACACTGCA GACCAGGTCA TCGCCTCATT TAAGATCCTG 
GCTGGAGACA AGAACTACAT CACAGCCGAG GAGCTGAGGC GTGAGCTTCC TCCAGATCAG 
GCAGAGTACT GCATCGCCCG AATGGCGCCG TACTCTGGGC CGGATGCAGT GGCTGGCGCT 
CTTGACTACA TGTCCTTCTC CACCGCCCTG TACGGGGAGA GCGACCTCTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

New classes of mind bomb-interacting proteins identified from yeast two-hybrid screens.
PloS one9(4)e93394(2014)
Tseng LC,Zhang C,Cheng CM,Xu H,Hsu CH,Jiang YJ

?-Actinin-2 deficiency results in sarcomeric defects in zebrafish that cannot be rescued by ?-actinin-3 revealing functional differences between sarcomeric isoforms.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology26(5)1892-908(2012 May)
Gupta V,Discenza M,Guyon JR,Kunkel LM,Beggs AH

Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung.
PloS one6(8)e24019(2011)
Zheng W,Wang Z,Collins JE,Andrews RM,Stemple D,Gong Z

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actn4 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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