ACTN4 cDNA ORF clone, Bos taurus(cattle)

The following ACTN4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTN4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OBa43743 NM_001098052.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), mRNA. pcDNA3.1-C-(k)DYK or customized vector 12-14 $510.30
$729.00
OBa225039 XM_024978279.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225040 XM_024978280.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225041 XM_024978281.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225042 XM_024978282.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225043 XM_024978283.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225044 XM_024978284.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225045 XM_024978285.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225046 XM_024978286.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225047 XM_024978288.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X11, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $475.30
$679.00
OBa225037 XM_024978277.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00
OBa225038 XM_024978278.1
Latest version!
Bos taurus actinin alpha 4 (ACTN4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $559.30
$799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OBa43743
    Clone ID Related Accession (Same CDS sequence) NM_001098052.1
    Accession Version NM_001098052.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-12-25
    Organism Bos taurus(cattle)
    Product alpha-actinin-4
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC140512.1. On or before May 24, 2007 this sequence version replaced XM_001252980.1, XM_001253010.1, XM_872287.2, XM_001253060.1, XM_001253079.1, XM_872491.2. ##Evidence-Data-START## CDS exon combination :: BC140512.1 [ECO:0000331] RNAseq introns :: single sample supports all introns SAMN03145412, SAMN03145413 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACGCAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAACGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGGG CGATGCCGAG TTCAACCGCA TCATGAGCGT AGTGGACCCC
    AACCACAGCG GCCTCGTGAC CTTCCAAGCC TTCATCGACT TCATGTCGAG GGAGACCACC
    GACACAGACA CGGCCGACCA GGTCATCGCC TCCTTCAAGG TCCTGGCTGG GGACAAGAAC
    TTCATCACAG CCGAGGAGCT GCGGAGAGAG CTGCCGCCCG ACCAGGCCGA GTACTGCATC
    GCCCGCATGG CGCCCTACCA GGGCCCTGAC GCCGTGCCTG GCGCCCTCGA CTATAAGTCC
    TTCTCCACAG CCCTGTACGG AGAGAGCGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001091521.1
    CDS40..2775
    Translation

    Target ORF information:

    RefSeq Version NM_001098052.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001098052.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACGCAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAACGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGGG CGATGCCGAG TTCAACCGCA TCATGAGCGT AGTGGACCCC
    AACCACAGCG GCCTCGTGAC CTTCCAAGCC TTCATCGACT TCATGTCGAG GGAGACCACC
    GACACAGACA CGGCCGACCA GGTCATCGCC TCCTTCAAGG TCCTGGCTGG GGACAAGAAC
    TTCATCACAG CCGAGGAGCT GCGGAGAGAG CTGCCGCCCG ACCAGGCCGA GTACTGCATC
    GCCCGCATGG CGCCCTACCA GGGCCCTGAC GCCGTGCCTG GCGCCCTCGA CTATAAGTCC
    TTCTCCACAG CCCTGTACGG AGAGAGCGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225039
    Clone ID Related Accession (Same CDS sequence) XM_024978279.1
    Accession Version XM_024978279.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGACATCG TGAACACGGC CCGGCCCGAC
    GAGAAGGCCA TAATGACCTA TGTGTCCAGC TTCTACCATG CCTTTTCGGG AGCGCAGAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGG GCGATGCCGA GTTCAACCGC
    ATCATGAGCG TAGTGGACCC CAACCACAGC GGCCTCGTGA CCTTCCAAGC CTTCATCGAC
    TTCATGTCGA GGGAGACCAC CGACACAGAC ACGGCCGACC AGGTCATCGC CTCCTTCAAG
    GTCCTGGCTG GGGACAAGAA CTTCATCACA GCCGAGGAGC TGCGGAGAGA GCTGCCGCCC
    GACCAGGCCG AGTACTGCAT CGCCCGCATG GCGCCCTACC AGGGCCCTGA CGCCGTGCCT
    GGCGCCCTCG ACTATAAGTC CTTCTCCACA GCCCTGTACG GAGAGAGCGA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834047.1
    CDS16..3012
    Translation

    Target ORF information:

    RefSeq Version XM_024978279.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978279.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGACATCG TGAACACGGC CCGGCCCGAC
    GAGAAGGCCA TAATGACCTA TGTGTCCAGC TTCTACCATG CCTTTTCGGG AGCGCAGAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGG GCGATGCCGA GTTCAACCGC
    ATCATGAGCG TAGTGGACCC CAACCACAGC GGCCTCGTGA CCTTCCAAGC CTTCATCGAC
    TTCATGTCGA GGGAGACCAC CGACACAGAC ACGGCCGACC AGGTCATCGC CTCCTTCAAG
    GTCCTGGCTG GGGACAAGAA CTTCATCACA GCCGAGGAGC TGCGGAGAGA GCTGCCGCCC
    GACCAGGCCG AGTACTGCAT CGCCCGCATG GCGCCCTACC AGGGCCCTGA CGCCGTGCCT
    GGCGCCCTCG ACTATAAGTC CTTCTCCACA GCCCTGTACG GAGAGAGCGA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225040
    Clone ID Related Accession (Same CDS sequence) XM_024978280.1
    Accession Version XM_024978280.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2997bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGATATTG TAGGCACTCT GAGGCCAGAT
    GAGAAAGCCA TCATGACTTA CGTGTCCTGC TTCTACCACG CTTTCTCAGG GGCTCAAAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGG GCGATGCCGA GTTCAACCGC
    ATCATGAGCG TAGTGGACCC CAACCACAGC GGCCTCGTGA CCTTCCAAGC CTTCATCGAC
    TTCATGTCGA GGGAGACCAC CGACACAGAC ACGGCCGACC AGGTCATCGC CTCCTTCAAG
    GTCCTGGCTG GGGACAAGAA CTTCATCACA GCCGAGGAGC TGCGGAGAGA GCTGCCGCCC
    GACCAGGCCG AGTACTGCAT CGCCCGCATG GCGCCCTACC AGGGCCCTGA CGCCGTGCCT
    GGCGCCCTCG ACTATAAGTC CTTCTCCACA GCCCTGTACG GAGAGAGCGA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834048.1
    CDS16..3012
    Translation

    Target ORF information:

    RefSeq Version XM_024978280.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978280.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGATATTG TAGGCACTCT GAGGCCAGAT
    GAGAAAGCCA TCATGACTTA CGTGTCCTGC TTCTACCACG CTTTCTCAGG GGCTCAAAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGG GCGATGCCGA GTTCAACCGC
    ATCATGAGCG TAGTGGACCC CAACCACAGC GGCCTCGTGA CCTTCCAAGC CTTCATCGAC
    TTCATGTCGA GGGAGACCAC CGACACAGAC ACGGCCGACC AGGTCATCGC CTCCTTCAAG
    GTCCTGGCTG GGGACAAGAA CTTCATCACA GCCGAGGAGC TGCGGAGAGA GCTGCCGCCC
    GACCAGGCCG AGTACTGCAT CGCCCGCATG GCGCCCTACC AGGGCCCTGA CGCCGTGCCT
    GGCGCCCTCG ACTATAAGTC CTTCTCCACA GCCCTGTACG GAGAGAGCGA CCTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225041
    Clone ID Related Accession (Same CDS sequence) XM_024978281.1
    Accession Version XM_024978281.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGACATCG TGAACACGGC CCGGCCCGAC
    GAGAAGGCCA TAATGACCTA TGTGTCCAGC TTCTACCATG CCTTTTCGGG AGCGCAGAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834049.1
    CDS16..2997
    Translation

    Target ORF information:

    RefSeq Version XM_024978281.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978281.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGACATCG TGAACACGGC CCGGCCCGAC
    GAGAAGGCCA TAATGACCTA TGTGTCCAGC TTCTACCATG CCTTTTCGGG AGCGCAGAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225042
    Clone ID Related Accession (Same CDS sequence) XM_024978282.1
    Accession Version XM_024978282.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2982bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGATATTG TAGGCACTCT GAGGCCAGAT
    GAGAAAGCCA TCATGACTTA CGTGTCCTGC TTCTACCACG CTTTCTCAGG GGCTCAAAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834050.1
    CDS16..2997
    Translation

    Target ORF information:

    RefSeq Version XM_024978282.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978282.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGATATTG TAGGCACTCT GAGGCCAGAT
    GAGAAAGCCA TCATGACTTA CGTGTCCTGC TTCTACCACG CTTTCTCAGG GGCTCAAAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225043
    Clone ID Related Accession (Same CDS sequence) XM_024978283.1
    Accession Version XM_024978283.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834051.1
    CDS84..2885
    Translation

    Target ORF information:

    RefSeq Version XM_024978283.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978283.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225044
    Clone ID Related Accession (Same CDS sequence) XM_024978284.1
    Accession Version XM_024978284.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2802bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCAGGG GCTCAAAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834052.1
    CDS91..2892
    Translation

    Target ORF information:

    RefSeq Version XM_024978284.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978284.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCAGGG GCTCAAAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGAA GCAGACAGGC AGCATGGACT CCGATGACTT CAGGGCTCTG
    CTTATCTCCA CAGGATACAG CCTGGGCGAT GCCGAGTTCA ACCGCATCAT GAGCGTAGTG
    GACCCCAACC ACAGCGGCCT CGTGACCTTC CAAGCCTTCA TCGACTTCAT GTCGAGGGAG
    ACCACCGACA CAGACACGGC CGACCAGGTC ATCGCCTCCT TCAAGGTCCT GGCTGGGGAC
    AAGAACTTCA TCACAGCCGA GGAGCTGCGG AGAGAGCTGC CGCCCGACCA GGCCGAGTAC
    TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGACGCCG TGCCTGGCGC CCTCGACTAT
    AAGTCCTTCT CCACAGCCCT GTACGGAGAG AGCGACCTGT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225045
    Clone ID Related Accession (Same CDS sequence) XM_024978285.1
    Accession Version XM_024978285.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2736bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCAGGG GCTCAAAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGGG CGATGCCGAG TTCAACCGCA TCATGAGCGT AGTGGACCCC
    AACCACAGCG GCCTCGTGAC CTTCCAAGCC TTCATCGACT TCATGTCGAG GGAGACCACC
    GACACAGACA CGGCCGACCA GGTCATCGCC TCCTTCAAGG TCCTGGCTGG GGACAAGAAC
    TTCATCACAG CCGAGGAGCT GCGGAGAGAG CTGCCGCCCG ACCAGGCCGA GTACTGCATC
    GCCCGCATGG CGCCCTACCA GGGCCCTGAC GCCGTGCCTG GCGCCCTCGA CTATAAGTCC
    TTCTCCACAG CCCTGTACGG AGAGAGCGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834053.1
    CDS84..2819
    Translation

    Target ORF information:

    RefSeq Version XM_024978285.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978285.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCAGGG GCTCAAAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGGAC
    CACGGCGGGG CGCTGGGGCC GGAGGAGTTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC
    GTGGAGAACG ACCGGCAGGG CGATGCCGAG TTCAACCGCA TCATGAGCGT AGTGGACCCC
    AACCACAGCG GCCTCGTGAC CTTCCAAGCC TTCATCGACT TCATGTCGAG GGAGACCACC
    GACACAGACA CGGCCGACCA GGTCATCGCC TCCTTCAAGG TCCTGGCTGG GGACAAGAAC
    TTCATCACAG CCGAGGAGCT GCGGAGAGAG CTGCCGCCCG ACCAGGCCGA GTACTGCATC
    GCCCGCATGG CGCCCTACCA GGGCCCTGAC GCCGTGCCTG GCGCCCTCGA CTATAAGTCC
    TTCTCCACAG CCCTGTACGG AGAGAGCGAC CTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225046
    Clone ID Related Accession (Same CDS sequence) XM_024978286.1
    Accession Version XM_024978286.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGCGATG CCGAGTTCAA CCGCATCATG AGCGTAGTGG ACCCCAACCA CAGCGGCCTC
    GTGACCTTCC AAGCCTTCAT CGACTTCATG TCGAGGGAGA CCACCGACAC AGACACGGCC
    GACCAGGTCA TCGCCTCCTT CAAGGTCCTG GCTGGGGACA AGAACTTCAT CACAGCCGAG
    GAGCTGCGGA GAGAGCTGCC GCCCGACCAG GCCGAGTACT GCATCGCCCG CATGGCGCCC
    TACCAGGGCC CTGACGCCGT GCCTGGCGCC CTCGACTATA AGTCCTTCTC CACAGCCCTG
    TACGGAGAGA GCGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834054.1
    CDS84..2804
    Translation

    Target ORF information:

    RefSeq Version XM_024978286.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978286.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGACATCGT GAACACGGCC CGGCCCGACG AGAAGGCCAT AATGACCTAT
    GTGTCCAGCT TCTACCATGC CTTTTCGGGA GCGCAGAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGCGATG CCGAGTTCAA CCGCATCATG AGCGTAGTGG ACCCCAACCA CAGCGGCCTC
    GTGACCTTCC AAGCCTTCAT CGACTTCATG TCGAGGGAGA CCACCGACAC AGACACGGCC
    GACCAGGTCA TCGCCTCCTT CAAGGTCCTG GCTGGGGACA AGAACTTCAT CACAGCCGAG
    GAGCTGCGGA GAGAGCTGCC GCCCGACCAG GCCGAGTACT GCATCGCCCG CATGGCGCCC
    TACCAGGGCC CTGACGCCGT GCCTGGCGCC CTCGACTATA AGTCCTTCTC CACAGCCCTG
    TACGGAGAGA GCGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225047
    Clone ID Related Accession (Same CDS sequence) XM_024978288.1
    Accession Version XM_024978288.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2721bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X11
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCAGGG GCTCAAAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGCGATG CCGAGTTCAA CCGCATCATG AGCGTAGTGG ACCCCAACCA CAGCGGCCTC
    GTGACCTTCC AAGCCTTCAT CGACTTCATG TCGAGGGAGA CCACCGACAC AGACACGGCC
    GACCAGGTCA TCGCCTCCTT CAAGGTCCTG GCTGGGGACA AGAACTTCAT CACAGCCGAG
    GAGCTGCGGA GAGAGCTGCC GCCCGACCAG GCCGAGTACT GCATCGCCCG CATGGCGCCC
    TACCAGGGCC CTGACGCCGT GCCTGGCGCC CTCGACTATA AGTCCTTCTC CACAGCCCTG
    TACGGAGAGA GCGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834056.1
    CDS118..2838
    Translation

    Target ORF information:

    RefSeq Version XM_024978288.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X11, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978288.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    ATGGTGGACT ACCACGCGGC GAACCAGTCG TACCAGTACG GCCCCAGCAG CGGGAGCAAT 
    GGCGCTGGCG GCGGGGGTAC CATGGGAGAC TACATGGCCC AGGAGGACGA CTGGGACCGG
    GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC
    AACTCCCACC TGCGGAAGGC CGGCACACAG ATCGAGAACA TTGATGAGGA CTTCCGAGAC
    GGGCTCAAGC TCATGCTCCT CCTGGAGGTC ATTTCAGGGG AGCGGTTACC TAAGCCAGAG
    CGGGGCAAGA TGAGAGTGCA CAAAATCAAC AACGTGAACA AGGCACTGGA CTTCATCGCC
    AGCAAAGGGG TCAAGCTGGT CTCCATCGGG GCGGAAGAGA TTGTGGACGG CAACGCGAAG
    ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAGGA CATCTCTGTG
    GAAGAGACCT CTGCCAAGGA GGGGCTCCTC CTCTGGTGCC AGAGAAAGAC GGCCCCGTAC
    AAGAATGTCA ACGTGCAGAA CTTCCACATC AGCTGGAAGG ATGGTCTTGC CTTCAATGCC
    CTGATCCACC GGCACAGACC AGAGCTGATC GAGTACGACA AGCTGAGAAA GGACGACCCC
    GTCACCAACC TGAACAATGC CTTCGAAGTG GCTGAGAAAT ACCTCGACAT CCCCAAGATG
    CTAGATGCAG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAAGCCAT CATGACTTAC
    GTGTCCTGCT TCTACCACGC TTTCTCAGGG GCTCAAAAGG CTGAGACCGC TGCCAACCGG
    ATCTGCAAGG TGCTGGCCGT CAACCAGGAG AACGAGCACC TGATGGAAGA TTATGAGAGG
    CTGGCCAGCG ACCTCCTGGA GTGGATCCGG CGCACCATCC CCTGGCTGGA GGACCGCGTG
    CCGCAGAAAA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGCGA CTACCGGCGG
    GTCCACAAGC CGCCGAAGGT GCAGGAGAAG TGCCAGCTGG AGATCAACTT CAACACGCTG
    CAGACCAAGC TGCGCCTCAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAAGATGGTG
    TCGGACATCA ACAACGGCTG GCAGCACCTG GAGCAGGCCG AGAAGGGCTA CGAGGAGTGG
    CTGCTGAACG AGATCCGCCG GCTGGAGCGG CTCGACCACC TGGCAGAGAA GTTCCGGCAG
    AAGGCCTCCA TCCACGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC
    TATGAGACGG CCACCCTGTC GGACATCAAG GCCCTCATCC GCAAGCACGA AGCCTTCGAG
    AGCGACCTGG CCGCCCACCA GGACCGCGTG GAGCAGATTG CCGCCATCGC CCAGGAGCTC
    AACGAGCTGG ATTACTACGA CTCCCACAAC GTCAACACGC GCTGCCAGAA GATCTGTGAC
    CAGTGGGACG CCCTTGGCTC TCTGACCCAC AGTCGCAGGG AAGCCCTGGA GAAAACGGAG
    AAGCAGCTAG AGACCATCGA CCAGCTGCAC CTGGAGTACG CCAAGCGGGC AGCCCCCTTC
    AACAACTGGA TGGAGAGCGC CATGGAGGAC CTCCAGGACA TGTTCATCGT CCACACCATC
    GAGGAAATCG AGGGTCTGAT CTCAGCCCAC GACCAGTTCA AGTCGACGCT GCCAGATGCC
    GACAGGGAGC GGGAGGCCAT CCTGGCCATC CACAAGGAAG CCCAGCGGAT CGCCGAGAGC
    AACCACATCA AGCTCTCGGG CAGCAACCCC TACACCACCG TCACTCCCCA GATCATCAAT
    TCCAAGTGGG AGAAGGTGCA GCAGCTGGTG CCCAAGCGGG ACCACGCCCT CCTGGAGGAG
    CAGAGCAAAC AACAGTCCAA CGAGCACCTC CGCCGCCAGT TCGCCAGCCA GGCCAATATT
    GTGGGGCCCT GGATCCAGAC GAAGATGGAG GAGATCGGGC GCATCTCTAT CGAGATGAAT
    GGGACGCTGG AGGACCAGCT GAGCCACCTG AAGCAGTATG AGCGCAGCAT TGTGGACTAC
    AAGCCCAACC TGGACCTGCT CGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC
    GACAACAAGC ACACCAACTA TACCATGGAG CACATCCGGG TGGGCTGGGA GCAGCTGCTC
    ACCACCATCG CCCGCACCAT CAACGAGGTC GAGAACCAGA TCCTCACCCG CGACGCCAAG
    GGCATCAGCC AGGAGCAGAT GCAGGAGTTC CGGGCGTCCT TCAACCACTT CGACAAGAAG
    CAGACAGGCA GCATGGACTC CGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC
    CTGGGCGATG CCGAGTTCAA CCGCATCATG AGCGTAGTGG ACCCCAACCA CAGCGGCCTC
    GTGACCTTCC AAGCCTTCAT CGACTTCATG TCGAGGGAGA CCACCGACAC AGACACGGCC
    GACCAGGTCA TCGCCTCCTT CAAGGTCCTG GCTGGGGACA AGAACTTCAT CACAGCCGAG
    GAGCTGCGGA GAGAGCTGCC GCCCGACCAG GCCGAGTACT GCATCGCCCG CATGGCGCCC
    TACCAGGGCC CTGACGCCGT GCCTGGCGCC CTCGACTATA AGTCCTTCTC CACAGCCCTG
    TACGGAGAGA GCGACCTGTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225037
    Clone ID Related Accession (Same CDS sequence) XM_024978277.1
    Accession Version XM_024978277.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGACATCG TGAACACGGC CCGGCCCGAC
    GAGAAGGCCA TAATGACCTA TGTGTCCAGC TTCTACCATG CCTTTTCGGG AGCGCAGAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGA AGCAGACAGG CAGCATGGAC
    TCCGATGACT TCAGGGCTCT GCTTATCTCC ACAGGATACA GCCTGGGCGA TGCCGAGTTC
    AACCGCATCA TGAGCGTAGT GGACCCCAAC CACAGCGGCC TCGTGACCTT CCAAGCCTTC
    ATCGACTTCA TGTCGAGGGA GACCACCGAC ACAGACACGG CCGACCAGGT CATCGCCTCC
    TTCAAGGTCC TGGCTGGGGA CAAGAACTTC ATCACAGCCG AGGAGCTGCG GAGAGAGCTG
    CCGCCCGACC AGGCCGAGTA CTGCATCGCC CGCATGGCGC CCTACCAGGG CCCTGACGCC
    GTGCCTGGCG CCCTCGACTA TAAGTCCTTC TCCACAGCCC TGTACGGAGA GAGCGACCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834045.1
    CDS16..3078
    Translation

    Target ORF information:

    RefSeq Version XM_024978277.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978277.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGACATCG TGAACACGGC CCGGCCCGAC
    GAGAAGGCCA TAATGACCTA TGTGTCCAGC TTCTACCATG CCTTTTCGGG AGCGCAGAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGA AGCAGACAGG CAGCATGGAC
    TCCGATGACT TCAGGGCTCT GCTTATCTCC ACAGGATACA GCCTGGGCGA TGCCGAGTTC
    AACCGCATCA TGAGCGTAGT GGACCCCAAC CACAGCGGCC TCGTGACCTT CCAAGCCTTC
    ATCGACTTCA TGTCGAGGGA GACCACCGAC ACAGACACGG CCGACCAGGT CATCGCCTCC
    TTCAAGGTCC TGGCTGGGGA CAAGAACTTC ATCACAGCCG AGGAGCTGCG GAGAGAGCTG
    CCGCCCGACC AGGCCGAGTA CTGCATCGCC CGCATGGCGC CCTACCAGGG CCCTGACGCC
    GTGCCTGGCG CCCTCGACTA TAAGTCCTTC TCCACAGCCC TGTACGGAGA GAGCGACCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID OBa225038
    Clone ID Related Accession (Same CDS sequence) XM_024978278.1
    Accession Version XM_024978278.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3063bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-05-06
    Organism Bos taurus(cattle)
    Product alpha-actinin-4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037345.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGATATTG TAGGCACTCT GAGGCCAGAT
    GAGAAAGCCA TCATGACTTA CGTGTCCTGC TTCTACCACG CTTTCTCAGG GGCTCAAAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGA AGCAGACAGG CAGCATGGAC
    TCCGATGACT TCAGGGCTCT GCTTATCTCC ACAGGATACA GCCTGGGCGA TGCCGAGTTC
    AACCGCATCA TGAGCGTAGT GGACCCCAAC CACAGCGGCC TCGTGACCTT CCAAGCCTTC
    ATCGACTTCA TGTCGAGGGA GACCACCGAC ACAGACACGG CCGACCAGGT CATCGCCTCC
    TTCAAGGTCC TGGCTGGGGA CAAGAACTTC ATCACAGCCG AGGAGCTGCG GAGAGAGCTG
    CCGCCCGACC AGGCCGAGTA CTGCATCGCC CGCATGGCGC CCTACCAGGG CCCTGACGCC
    GTGCCTGGCG CCCTCGACTA TAAGTCCTTC TCCACAGCCC TGTACGGAGA GAGCGACCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_024834046.1
    CDS16..3078
    Translation

    Target ORF information:

    RefSeq Version XM_024978278.1
    Organism Bos taurus(cattle)
    Definition Bos taurus actinin alpha 4 (ACTN4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_024978278.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGAGCTGTT CTGGTGACTG TTGTCAAAAC ACATGGTGTT TGGCCAGTGT CTCCCTGGAA 
    GCCCCTCGCC CCCCACACGG CCTCTGCCCA CACTCGGGCG CAGCGCGAGC TCTCGTGCAT
    TCCTGGCCAT CGGCCCAGGT CCACGCTGGG CCTGCAGGCT GGTGGGGACA GGGCAGGGGA
    CTCCCGTGGG AAACAGGCGT GGGCTTTTTC CCAGACCCTG CTGCTGGGGC TGCGTGGGCC
    GGTCTCCCCG GAAGGTGTCT GGACACGTCC ACCTTAACTG ATGTGACGCA CTTCCCTGGC
    GCTGGAGTCG ATGACGACAG AGGCCCCAGC CAGCAGGGGG CTGAGCGCCA GTCACCTTGG
    ACGCCACGCC CGGGCCCCAT CCTCCGGTGT TCTCTGCTCT GGCTCAGGAC TTGGGCTGCT
    GATACCTTCA CAGCCTGGTG CAACTCCCAC CTGCGGAAGG CCGGCACACA GATCGAGAAC
    ATTGATGAGG ACTTCCGAGA CGGGCTCAAG CTCATGCTCC TCCTGGAGGT CATTTCAGGG
    GAGCGGTTAC CTAAGCCAGA GCGGGGCAAG ATGAGAGTGC ACAAAATCAA CAACGTGAAC
    AAGGCACTGG ACTTCATCGC CAGCAAAGGG GTCAAGCTGG TCTCCATCGG GGCGGAAGAG
    ATTGTGGACG GCAACGCGAA GATGACCCTG GGAATGATCT GGACCATCAT CCTCAGGTTC
    GCCATCCAGG ACATCTCTGT GGAAGAGACC TCTGCCAAGG AGGGGCTCCT CCTCTGGTGC
    CAGAGAAAGA CGGCCCCGTA CAAGAATGTC AACGTGCAGA ACTTCCACAT CAGCTGGAAG
    GATGGTCTTG CCTTCAATGC CCTGATCCAC CGGCACAGAC CAGAGCTGAT CGAGTACGAC
    AAGCTGAGAA AGGACGACCC CGTCACCAAC CTGAACAATG CCTTCGAAGT GGCTGAGAAA
    TACCTCGACA TCCCCAAGAT GCTAGATGCA GAGGATATTG TAGGCACTCT GAGGCCAGAT
    GAGAAAGCCA TCATGACTTA CGTGTCCTGC TTCTACCACG CTTTCTCAGG GGCTCAAAAG
    GCTGAGACCG CTGCCAACCG GATCTGCAAG GTGCTGGCCG TCAACCAGGA GAACGAGCAC
    CTGATGGAAG ATTATGAGAG GCTGGCCAGC GACCTCCTGG AGTGGATCCG GCGCACCATC
    CCCTGGCTGG AGGACCGCGT GCCGCAGAAA ACCATCCAGG AGATGCAGCA GAAGCTGGAG
    GACTTCCGCG ACTACCGGCG GGTCCACAAG CCGCCGAAGG TGCAGGAGAA GTGCCAGCTG
    GAGATCAACT TCAACACGCT GCAGACCAAG CTGCGCCTCA GCAACCGGCC TGCCTTCATG
    CCCTCCGAGG GCAAGATGGT GTCGGACATC AACAACGGCT GGCAGCACCT GGAGCAGGCC
    GAGAAGGGCT ACGAGGAGTG GCTGCTGAAC GAGATCCGCC GGCTGGAGCG GCTCGACCAC
    CTGGCAGAGA AGTTCCGGCA GAAGGCCTCC ATCCACGAGG CCTGGACCGA TGGGAAGGAG
    GCCATGCTGA AGCACCGGGA CTATGAGACG GCCACCCTGT CGGACATCAA GGCCCTCATC
    CGCAAGCACG AAGCCTTCGA GAGCGACCTG GCCGCCCACC AGGACCGCGT GGAGCAGATT
    GCCGCCATCG CCCAGGAGCT CAACGAGCTG GATTACTACG ACTCCCACAA CGTCAACACG
    CGCTGCCAGA AGATCTGTGA CCAGTGGGAC GCCCTTGGCT CTCTGACCCA CAGTCGCAGG
    GAAGCCCTGG AGAAAACGGA GAAGCAGCTA GAGACCATCG ACCAGCTGCA CCTGGAGTAC
    GCCAAGCGGG CAGCCCCCTT CAACAACTGG ATGGAGAGCG CCATGGAGGA CCTCCAGGAC
    ATGTTCATCG TCCACACCAT CGAGGAAATC GAGGGTCTGA TCTCAGCCCA CGACCAGTTC
    AAGTCGACGC TGCCAGATGC CGACAGGGAG CGGGAGGCCA TCCTGGCCAT CCACAAGGAA
    GCCCAGCGGA TCGCCGAGAG CAACCACATC AAGCTCTCGG GCAGCAACCC CTACACCACC
    GTCACTCCCC AGATCATCAA TTCCAAGTGG GAGAAGGTGC AGCAGCTGGT GCCCAAGCGG
    GACCACGCCC TCCTGGAGGA GCAGAGCAAA CAACAGTCCA ACGAGCACCT CCGCCGCCAG
    TTCGCCAGCC AGGCCAATAT TGTGGGGCCC TGGATCCAGA CGAAGATGGA GGAGATCGGG
    CGCATCTCTA TCGAGATGAA TGGGACGCTG GAGGACCAGC TGAGCCACCT GAAGCAGTAT
    GAGCGCAGCA TTGTGGACTA CAAGCCCAAC CTGGACCTGC TCGAGCAGCA GCACCAGCTC
    ATCCAGGAGG CCCTCATCTT CGACAACAAG CACACCAACT ATACCATGGA GCACATCCGG
    GTGGGCTGGG AGCAGCTGCT CACCACCATC GCCCGCACCA TCAACGAGGT CGAGAACCAG
    ATCCTCACCC GCGACGCCAA GGGCATCAGC CAGGAGCAGA TGCAGGAGTT CCGGGCGTCC
    TTCAACCACT TCGACAAGGA CCACGGCGGG GCGCTGGGGC CGGAGGAGTT CAAGGCCTGC
    CTCATCAGCC TGGGCTACGA CGTGGAGAAC GACCGGCAGA AGCAGACAGG CAGCATGGAC
    TCCGATGACT TCAGGGCTCT GCTTATCTCC ACAGGATACA GCCTGGGCGA TGCCGAGTTC
    AACCGCATCA TGAGCGTAGT GGACCCCAAC CACAGCGGCC TCGTGACCTT CCAAGCCTTC
    ATCGACTTCA TGTCGAGGGA GACCACCGAC ACAGACACGG CCGACCAGGT CATCGCCTCC
    TTCAAGGTCC TGGCTGGGGA CAAGAACTTC ATCACAGCCG AGGAGCTGCG GAGAGAGCTG
    CCGCCCGACC AGGCCGAGTA CTGCATCGCC CGCATGGCGC CCTACCAGGG CCCTGACGCC
    GTGCCTGGCG CCCTCGACTA TAAGTCCTTC TCCACAGCCC TGTACGGAGA GAGCGACCTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.