Actn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actn4
Entrez Gene ID	63836
Full Name	actinin alpha 4
General protein information	Preferred Names actinin alpha 4 Names alpha-actinin-4 F-actin cross-linking protein non-muscle alpha-actinin 4
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 1 Map Location: 1q21
Summary	alpha-actinin cytoskeletal protein that anchors various actin-containing microfilaments to cellular structures; anchors V myosins to cellular structures via the RING finger-containing protein BERP [RGD, Feb 2006].

mRNA and Protein(s)

mRNA	Protein	Name
XM_006228689.2	XP_006228751.1	alpha-actinin-4 isoform X3
NM_031675.2	NP_113863.2	alpha-actinin-4
XM_006228690.3	XP_006228752.1	alpha-actinin-4 isoform X4
XM_008759185.1	XP_008757407.1	alpha-actinin-4 isoform X5
XM_006228687.2	XP_006228749.1	alpha-actinin-4 isoform X1
XM_006228688.2	XP_006228750.1	alpha-actinin-4 isoform X2

Pathways from BioSystems

Homologies

	ACTN4	XP_001083825.1
Danio rerio (zebrafish)	actn4	NP_955880.1
Pan troglodytes (chimpanzee)	ACTN4	XP_003954421.1
Rattus norvegicus (Norway rat)	Actn4	NP_113863.2
Homo sapiens (human)	ACTN4	NP_004915.2
Bos taurus (cattle)	ACTN4	NP_001091521.1
Schizosaccharomyces pombe (fission yeast)	ain1	NP_594295.1
Canis lupus familiaris (dog)	ACTN4	XP_541640.2
Mus musculus (house mouse)	Actn4	NP_068695.1
Gallus gallus (chicken)	ACTN4	NP_990457.1
Magnaporthe oryzae (rice blast fungus)	MGG_06475	XP_003717088.1
Neurospora crassa	NCU06429	XP_957320.1
Xenopus tropicalis (tropical clawed frog)	actn4	NP_001006810.1

Gene Ontology
Bibliography

Related articles in PubMed

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting ?-Actinin 4 binding to actin filaments.
Ge H, Yu A, Chen J, Yuan J, Yin Y, Duanmu W, Tan L, Yang Y, Lan C, Chen W, Feng H, Hu R
Scientific reports637681(2016 Nov)

A complex of Rab13 with MICAL-L2 and ?-actinin-4 is essential for insulin-dependent GLUT4 exocytosis.
Sun Y, Jaldin-Fincati J, Liu Z, Bilan PJ, Klip A
Molecular biology of the cell27(1)75-89(2016 Jan)

Alpha-actinin binding kinetics modulate cellular dynamics and force generation.
Ehrlicher AJ, Krishnan R, Guo M, Bidan CM, Weitz DA, Pollak MR
Proceedings of the National Academy of Sciences of the United States of America112(21)6619-24(2015 May)

?-Actinin 4 potentiates nuclear factor ?-light-chain-enhancer of activated B-cell (NF-?B) activity in podocytes independent of its cytoplasmic actin binding function.
Zhao X, Hsu KS, Lim JH, Bruggeman LA, Kao HY
The Journal of biological chemistry290(1)338-49(2015 Jan)

Possible role of non-muscle alpha-actinins in muscle cell mechanosensitivity.
Ogneva IV, Biryukov NS, Leinsoo TA, Larina IM
PloS one9(4)e96395(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Findings suggest that MICAL-L2 is an effector of insulin-activated Rab13, which links to GLUT4 through ACTN4, localizing GLUT4 vesicles at the muscle cell periphery to enable their fusion with the membrane.
Title: A complex of Rab13 with MICAL-L2 and ?-actinin-4 is essential for insulin-dependent GLUT4 exocytosis.

After 6 hours of antiorthostatic suspension alpha-actinin 1 and alpha-actinin 4 levels decreased in the membranous fraction of proteins of cardiomyocytes and soleus muscle fibers.
Title: Possible role of non-muscle alpha-actinins in muscle cell mechanosensitivity.

Together, these results indicate that alpha-actinin and the actin cytoskeleton are important components of the cisternal organelle that are probably required to stabilize the AIS.
Title: Colocalization of ?-actinin and synaptopodin in the pyramidal cell axon initial segment.

The calcium sensitivity of alpha-actinin is required for its equatorial accumulation that is crucial for the initial equatorial actin assembly but is dispensable for cytokinesis.
Title: Calcium sensitivity of ?-actinin is required for equatorial actin assembly during cytokinesis.

Alpha-actinin-4 K256E triggers several metabolic abnormalities, which may lead to glomerular epithelial cell injury and glomerulosclerosis.
Title: Glomerular epithelial cell injury associated with mutant alpha-actinin-4.

The following Actn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa08447	NM_031675.2 Latest version!	Rattus norvegicus actinin alpha 4 (Actn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$538.30 $769.00
ORa22548	XM_006228690.3 Latest version!	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$391.30 $559.00
ORa22545	XM_006228687.2 Latest version!	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22546	XM_006228688.2 Latest version!	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa22547	XM_006228689.2 Latest version!	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
ORa43538	XM_008759185.1 Latest version!	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actn4 ( NM_031675.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_113863.2 Rattus norvegicus actinin alpha 4 (Actn4), mRNA.

Price & Availability↑

CloneID	ORa08447
Clone ID Related Accession (Same CDS sequence)	NM_031675.2
Accession Version	NM_031675.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-26
Organism	Rattus norvegicus(Norway rat)
Product	alpha-actinin-4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CB805703.1, BC061788.1, BQ206143.1 and AI070095.1. On Oct 12, 2005 this sequence version replaced NM_031675.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: BC061788.1, AY724534.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00052296, SAMD00052297 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGACATCGT GAACACAGCC CGGCCCGACG AGAAGGCCAT AATGACATAT 
GTGTCCAGCT TCTACCATGC CTTTTCAGGA GCGCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGGAC 
CATGGCGGGG CACTGGGGCC TGAGGAATTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC 
GTGGAGAATG ACCGGCAGGG TGATGCTGAG TTCAACCGGA TCATGAGTGT GGTCGACCCC 
AACCATAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGACT TCATGTCAAG GGAGACCACA 
GACACAGACA CCGCCGATCA GGTCATCGCC TCCTTCAAGG TCCTGGCCGG GGACAAGAAC 
TTCATCACTG CTGAGGAACT TCGGAGAGAG CTGCCCCCTG ACCAGGCTGA GTACTGCATC 
GCCCGCATGG CGCCCTACCA GGGCCCTGAT GCTGCTCCAG GCGCCCTTGA CTACAAGTCC 
TTCTCTACAG CCCTCTATGG GGAGAGCGAC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_113863.2
CDS	109..2844
Translation	MVDYHAANQAYQYGPSSGGNGTGGGGGMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_031675.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actinin alpha 4 (Actn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_031675.2

ORF Insert Sequence:

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGACATCGT GAACACAGCC CGGCCCGACG AGAAGGCCAT AATGACATAT 
GTGTCCAGCT TCTACCATGC CTTTTCAGGA GCGCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGGAC 
CATGGCGGGG CACTGGGGCC TGAGGAATTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC 
GTGGAGAATG ACCGGCAGGG TGATGCTGAG TTCAACCGGA TCATGAGTGT GGTCGACCCC 
AACCATAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGACT TCATGTCAAG GGAGACCACA 
GACACAGACA CCGCCGATCA GGTCATCGCC TCCTTCAAGG TCCTGGCCGG GGACAAGAAC 
TTCATCACTG CTGAGGAACT TCGGAGAGAG CTGCCCCCTG ACCAGGCTGA GTACTGCATC 
GCCCGCATGG CGCCCTACCA GGGCCCTGAT GCTGCTCCAG GCGCCCTTGA CTACAAGTCC 
TTCTCTACAG CCCTCTATGG GGAGAGCGAC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actn4 ( XM_006228690.3 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228752.1 Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ORa22548
Clone ID Related Accession (Same CDS sequence)	XM_006228690.3
Accession Version	XM_006228690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2484bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-actinin-4 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228690.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTGC TGGAGGTCAT TTCAGGGGAG CGGTTGCCTA AGCCAGAGCG GGGGAAGATG 
CGAGTGCACA AGATCAACAA TGTAAACAAA GCCCTGGACT TCATCGCCAG CAAGGGAGTC 
AAGCTGGTGT CCATTGGGGC AGAAGAGATC GTGGATGGCA ATGCAAAGAT GACCCTGGGA 
ATGATCTGGA CCATCATCCT CAGGTTCGCC ATCCAAGACA TCTCTGTGGA AGAGACCTCT 
GCCAAGGAAG GACTCCTCCT CTGGTGCCAG AGAAAGACAG CCCCGTATAA AAACGTCAAC 
GTCCAGAACT TCCATATCAG CTGGAAGGAT GGTCTGGCCT TCAACGCGCT CATCCACCGG 
CACAGACCTG AGCTGATTGA GTATGACAAA CTGAGGAAGG ATGATCCAGT CACCAACCTA 
AACAATGCAT TTGAAGTGGC TGAGAAATAC CTTGATATCC CCAAGATGTT GGATGCTGAG 
GACATCGTGA ACACAGCCCG GCCCGACGAG AAGGCCATAA TGACATATGT GTCCAGCTTC 
TACCATGCCT TTTCAGGAGC GCAGAAGGCT GAGACTGCTG CCAACCGGAT CTGCAAGGTG 
CTGGCTGTGA ACCAGGAAAA TGAGCACCTG ATGGAAGATT ACGAGCGCCT GGCCAGTGAT 
CTTCTAGAGT GGATCCGGCG CACCATCCCC TGGCTGGAAG ACCGTGTGCC TCAGAAGACC 
ATCCAGGAGA TGCAGCAGAA GCTGGAGGAC TTCCGAGACT ATAGGCGTGT GCACAAGCCC 
CCCAAGGTAC AGGAGAAGTG TCAGCTGGAG ATCAACTTCA ACACCCTGCA GACCAAGCTG 
CGGCTGAGCA ACCGGCCTGC CTTCATGCCC TCCGAGGGCA GGATGGTCTC GGACATCAAC 
AATGGGTGGC AACATCTGGA GCAGGCTGAG AAGGGCTATG AAGAATGGCT CTTGAATGAA 
ATCCGTAGGC TGGAACGACT TGATCACCTG GCAGAGAAAT TCCGGCAGAA GGCTTCCATC 
CATGAGGCCT GGACCGATGG GAAGGAGGCC ATGCTGAAGC ACCGGGACTA TGAGACAGCC 
ACCCTGTCAG ACATCAAAGC TCTGATCCGA AAGCATGAGG CCTTTGAGAG TGACCTGGCG 
GCGCACCAGG ACCGCGTGGA GCAGATCGCG GCTATTGCCC AGGAGCTCAA CGAACTGGAC 
TACTATGACT CCCACAACGT CAACACACGG TGCCAGAAGA TCTGTGACCA GTGGGATAAC 
CTGGGCTCTC TGACCCACAG TCGCAGGGAA GCCTTGGAGA AAACAGAGAA ACAGCTGGAG 
ACCATCGACC AGCTACACTT GGAGTATGCT AAGCGGGCCG CGCCCTTCAA CAACTGGATG 
GAGAGTGCCA TGGAGGACCT GCAGGACATG TTCATCGTCC ACACCATCGA GGAGATTGAG 
GGCCTGATCT CAGCCCACGA CCAGTTCAAG TCCACCCTGC CAGATGCCGA CAGGGAGCGT 
GAGGCCATCC TGGCCATCCA CAAAGAGGCC CAAAGGATCG CTGAGAGCAA TCACATCAAG 
CTGTCGGGCA GCAACCCCTA CACCAGCGTT ACACCCCAGA TCATCAACTC CAAATGGGAG 
AAGGTGCAGC AGCTGGTGCC GAAGCGGGAC CACGCACTCC TGGAAGAGCA GAGCAAGCAG 
CAATCCAATG AGCACCTTCG CCGACAGTTT GCCAGCCAAG CCAATATGGT GGGGCCCTGG 
ATCCAGACCA AGATGGAGGA GATTGGGCGC ATCTCCATCG AGATGAACGG GACTCTGGAA 
GACCAGCTGA GTCACTTGAA GCAGTACGAG CGCAGCATTG TGGACTACAA GCCCAACCTG 
GACTTGCTGG AGCAGCAGCA CCAGCTCATC CAGGAGGCCC TCATCTTCGA CAACAAGCAC 
ACCAACTACA CAATGGAGCA CCTCCGTGTG GGCTGGGAGC AGCTGCTCAC CACCATTGCC 
CGCACCATCA ACGAGGTAGA GAATCAGATC CTCACCCGAG ATGCCAAGGG CATCAGCCAG 
GAGCAAATGC AGGAGTTCCG AGCCTCCTTC AACCACTTTG ACAAGGACCA TGGCGGGGCA 
CTGGGGCCTG AGGAATTCAA GGCCTGCCTC ATCAGCCTGG GCTACGACGT GGAGAATGAC 
CGGCAGGGTG ATGCTGAGTT CAACCGGATC ATGAGTGTGG TCGACCCCAA CCATAGTGGC 
CTCGTGACCT TCCAAGCCTT CATTGACTTC ATGTCAAGGG AGACCACAGA CACAGACACC 
GCCGATCAGG TCATCGCCTC CTTCAAGGTC CTGGCCGGGG ACAAGAACTT CATCACTGCT 
GAGGAACTTC GGAGAGAGCT GCCCCCTGAC CAGGCTGAGT ACTGCATCGC CCGCATGGCG 
CCCTACCAGG GCCCTGATGC TGCTCCAGGC GCCCTTGACT ACAAGTCCTT CTCTACAGCC 
CTCTATGGGG AGAGCGACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228752.1
CDS	673..3156
Translation	MLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_006228690.3
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228690.3

ORF Insert Sequence:

ATGCTTCTGC TGGAGGTCAT TTCAGGGGAG CGGTTGCCTA AGCCAGAGCG GGGGAAGATG 
CGAGTGCACA AGATCAACAA TGTAAACAAA GCCCTGGACT TCATCGCCAG CAAGGGAGTC 
AAGCTGGTGT CCATTGGGGC AGAAGAGATC GTGGATGGCA ATGCAAAGAT GACCCTGGGA 
ATGATCTGGA CCATCATCCT CAGGTTCGCC ATCCAAGACA TCTCTGTGGA AGAGACCTCT 
GCCAAGGAAG GACTCCTCCT CTGGTGCCAG AGAAAGACAG CCCCGTATAA AAACGTCAAC 
GTCCAGAACT TCCATATCAG CTGGAAGGAT GGTCTGGCCT TCAACGCGCT CATCCACCGG 
CACAGACCTG AGCTGATTGA GTATGACAAA CTGAGGAAGG ATGATCCAGT CACCAACCTA 
AACAATGCAT TTGAAGTGGC TGAGAAATAC CTTGATATCC CCAAGATGTT GGATGCTGAG 
GACATCGTGA ACACAGCCCG GCCCGACGAG AAGGCCATAA TGACATATGT GTCCAGCTTC 
TACCATGCCT TTTCAGGAGC GCAGAAGGCT GAGACTGCTG CCAACCGGAT CTGCAAGGTG 
CTGGCTGTGA ACCAGGAAAA TGAGCACCTG ATGGAAGATT ACGAGCGCCT GGCCAGTGAT 
CTTCTAGAGT GGATCCGGCG CACCATCCCC TGGCTGGAAG ACCGTGTGCC TCAGAAGACC 
ATCCAGGAGA TGCAGCAGAA GCTGGAGGAC TTCCGAGACT ATAGGCGTGT GCACAAGCCC 
CCCAAGGTAC AGGAGAAGTG TCAGCTGGAG ATCAACTTCA ACACCCTGCA GACCAAGCTG 
CGGCTGAGCA ACCGGCCTGC CTTCATGCCC TCCGAGGGCA GGATGGTCTC GGACATCAAC 
AATGGGTGGC AACATCTGGA GCAGGCTGAG AAGGGCTATG AAGAATGGCT CTTGAATGAA 
ATCCGTAGGC TGGAACGACT TGATCACCTG GCAGAGAAAT TCCGGCAGAA GGCTTCCATC 
CATGAGGCCT GGACCGATGG GAAGGAGGCC ATGCTGAAGC ACCGGGACTA TGAGACAGCC 
ACCCTGTCAG ACATCAAAGC TCTGATCCGA AAGCATGAGG CCTTTGAGAG TGACCTGGCG 
GCGCACCAGG ACCGCGTGGA GCAGATCGCG GCTATTGCCC AGGAGCTCAA CGAACTGGAC 
TACTATGACT CCCACAACGT CAACACACGG TGCCAGAAGA TCTGTGACCA GTGGGATAAC 
CTGGGCTCTC TGACCCACAG TCGCAGGGAA GCCTTGGAGA AAACAGAGAA ACAGCTGGAG 
ACCATCGACC AGCTACACTT GGAGTATGCT AAGCGGGCCG CGCCCTTCAA CAACTGGATG 
GAGAGTGCCA TGGAGGACCT GCAGGACATG TTCATCGTCC ACACCATCGA GGAGATTGAG 
GGCCTGATCT CAGCCCACGA CCAGTTCAAG TCCACCCTGC CAGATGCCGA CAGGGAGCGT 
GAGGCCATCC TGGCCATCCA CAAAGAGGCC CAAAGGATCG CTGAGAGCAA TCACATCAAG 
CTGTCGGGCA GCAACCCCTA CACCAGCGTT ACACCCCAGA TCATCAACTC CAAATGGGAG 
AAGGTGCAGC AGCTGGTGCC GAAGCGGGAC CACGCACTCC TGGAAGAGCA GAGCAAGCAG 
CAATCCAATG AGCACCTTCG CCGACAGTTT GCCAGCCAAG CCAATATGGT GGGGCCCTGG 
ATCCAGACCA AGATGGAGGA GATTGGGCGC ATCTCCATCG AGATGAACGG GACTCTGGAA 
GACCAGCTGA GTCACTTGAA GCAGTACGAG CGCAGCATTG TGGACTACAA GCCCAACCTG 
GACTTGCTGG AGCAGCAGCA CCAGCTCATC CAGGAGGCCC TCATCTTCGA CAACAAGCAC 
ACCAACTACA CAATGGAGCA CCTCCGTGTG GGCTGGGAGC AGCTGCTCAC CACCATTGCC 
CGCACCATCA ACGAGGTAGA GAATCAGATC CTCACCCGAG ATGCCAAGGG CATCAGCCAG 
GAGCAAATGC AGGAGTTCCG AGCCTCCTTC AACCACTTTG ACAAGGACCA TGGCGGGGCA 
CTGGGGCCTG AGGAATTCAA GGCCTGCCTC ATCAGCCTGG GCTACGACGT GGAGAATGAC 
CGGCAGGGTG ATGCTGAGTT CAACCGGATC ATGAGTGTGG TCGACCCCAA CCATAGTGGC 
CTCGTGACCT TCCAAGCCTT CATTGACTTC ATGTCAAGGG AGACCACAGA CACAGACACC 
GCCGATCAGG TCATCGCCTC CTTCAAGGTC CTGGCCGGGG ACAAGAACTT CATCACTGCT 
GAGGAACTTC GGAGAGAGCT GCCCCCTGAC CAGGCTGAGT ACTGCATCGC CCGCATGGCG 
CCCTACCAGG GCCCTGATGC TGCTCCAGGC GCCCTTGACT ACAAGTCCTT CTCTACAGCC 
CTCTATGGGG AGAGCGACCT GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actn4 ( XM_006228687.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228749.1 Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa22545
Clone ID Related Accession (Same CDS sequence)	XM_006228687.2
Accession Version	XM_006228687.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2736bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-actinin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228687.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAGGCCAT CATGACTTAC 
GTGTCCTGCT TCTACCACGC CTTCTCGGGG GCCCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGGAC 
CATGGCGGGG CACTGGGGCC TGAGGAATTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC 
GTGGAGAATG ACCGGCAGGG TGATGCTGAG TTCAACCGGA TCATGAGTGT GGTCGACCCC 
AACCATAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGACT TCATGTCAAG GGAGACCACA 
GACACAGACA CCGCCGATCA GGTCATCGCC TCCTTCAAGG TCCTGGCCGG GGACAAGAAC 
TTCATCACTG CTGAGGAACT TCGGAGAGAG CTGCCCCCTG ACCAGGCTGA GTACTGCATC 
GCCCGCATGG CGCCCTACCA GGGCCCTGAT GCTGCTCCAG GCGCCCTTGA CTACAAGTCC 
TTCTCTACAG CCCTCTATGG GGAGAGCGAC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228749.1
CDS	148..2883
Translation	MVDYHAANQAYQYGPSSGGNGTGGGGGMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_006228687.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228687.2

ORF Insert Sequence:

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAGGCCAT CATGACTTAC 
GTGTCCTGCT TCTACCACGC CTTCTCGGGG GCCCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGGAC 
CATGGCGGGG CACTGGGGCC TGAGGAATTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC 
GTGGAGAATG ACCGGCAGGG TGATGCTGAG TTCAACCGGA TCATGAGTGT GGTCGACCCC 
AACCATAGTG GCCTCGTGAC CTTCCAAGCC TTCATTGACT TCATGTCAAG GGAGACCACA 
GACACAGACA CCGCCGATCA GGTCATCGCC TCCTTCAAGG TCCTGGCCGG GGACAAGAAC 
TTCATCACTG CTGAGGAACT TCGGAGAGAG CTGCCCCCTG ACCAGGCTGA GTACTGCATC 
GCCCGCATGG CGCCCTACCA GGGCCCTGAT GCTGCTCCAG GCGCCCTTGA CTACAAGTCC 
TTCTCTACAG CCCTCTATGG GGAGAGCGAC CTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actn4 ( XM_006228688.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228750.1 Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa22546
Clone ID Related Accession (Same CDS sequence)	XM_006228688.2
Accession Version	XM_006228688.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-actinin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228688.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGACATCGT GAACACAGCC CGGCCCGACG AGAAGGCCAT AATGACATAT 
GTGTCCAGCT TCTACCATGC CTTTTCAGGA GCGCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGAAG 
CAGACAGGCA GCATGGACTC AGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC 
CTGGGTGATG CTGAGTTCAA CCGGATCATG AGTGTGGTCG ACCCCAACCA TAGTGGCCTC 
GTGACCTTCC AAGCCTTCAT TGACTTCATG TCAAGGGAGA CCACAGACAC AGACACCGCC 
GATCAGGTCA TCGCCTCCTT CAAGGTCCTG GCCGGGGACA AGAACTTCAT CACTGCTGAG 
GAACTTCGGA GAGAGCTGCC CCCTGACCAG GCTGAGTACT GCATCGCCCG CATGGCGCCC 
TACCAGGGCC CTGATGCTGC TCCAGGCGCC CTTGACTACA AGTCCTTCTC TACAGCCCTC 
TATGGGGAGA GCGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228750.1
CDS	148..2868
Translation	MVDYHAANQAYQYGPSSGGNGTGGGGGMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKKQTGSMDSDDFRALLISTGYSLGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_006228688.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228688.2

ORF Insert Sequence:

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGACATCGT GAACACAGCC CGGCCCGACG AGAAGGCCAT AATGACATAT 
GTGTCCAGCT TCTACCATGC CTTTTCAGGA GCGCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGAAG 
CAGACAGGCA GCATGGACTC AGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC 
CTGGGTGATG CTGAGTTCAA CCGGATCATG AGTGTGGTCG ACCCCAACCA TAGTGGCCTC 
GTGACCTTCC AAGCCTTCAT TGACTTCATG TCAAGGGAGA CCACAGACAC AGACACCGCC 
GATCAGGTCA TCGCCTCCTT CAAGGTCCTG GCCGGGGACA AGAACTTCAT CACTGCTGAG 
GAACTTCGGA GAGAGCTGCC CCCTGACCAG GCTGAGTACT GCATCGCCCG CATGGCGCCC 
TACCAGGGCC CTGATGCTGC TCCAGGCGCC CTTGACTACA AGTCCTTCTC TACAGCCCTC 
TATGGGGAGA GCGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actn4 ( XM_006228689.2 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_006228751.1 Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ORa22547
Clone ID Related Accession (Same CDS sequence)	XM_006228689.2
Accession Version	XM_006228689.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-actinin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jul 26, 2016 this sequence version replaced XM_006228689.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAGGCCAT CATGACTTAC 
GTGTCCTGCT TCTACCACGC CTTCTCGGGG GCCCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGAAG 
CAGACAGGCA GCATGGACTC AGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC 
CTGGGTGATG CTGAGTTCAA CCGGATCATG AGTGTGGTCG ACCCCAACCA TAGTGGCCTC 
GTGACCTTCC AAGCCTTCAT TGACTTCATG TCAAGGGAGA CCACAGACAC AGACACCGCC 
GATCAGGTCA TCGCCTCCTT CAAGGTCCTG GCCGGGGACA AGAACTTCAT CACTGCTGAG 
GAACTTCGGA GAGAGCTGCC CCCTGACCAG GCTGAGTACT GCATCGCCCG CATGGCGCCC 
TACCAGGGCC CTGATGCTGC TCCAGGCGCC CTTGACTACA AGTCCTTCTC TACAGCCCTC 
TATGGGGAGA GCGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006228751.1
CDS	1..2721
Translation	MVDYHAANQAYQYGPSSGGNGTGGGGGMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKKQTGSMDSDDFRALLISTGYSLGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_006228689.2
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006228689.2

ORF Insert Sequence:

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGATATTGT AGGCACTCTG AGGCCAGATG AGAAGGCCAT CATGACTTAC 
GTGTCCTGCT TCTACCACGC CTTCTCGGGG GCCCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGAAG 
CAGACAGGCA GCATGGACTC AGATGACTTC AGGGCTCTGC TTATCTCCAC AGGATACAGC 
CTGGGTGATG CTGAGTTCAA CCGGATCATG AGTGTGGTCG ACCCCAACCA TAGTGGCCTC 
GTGACCTTCC AAGCCTTCAT TGACTTCATG TCAAGGGAGA CCACAGACAC AGACACCGCC 
GATCAGGTCA TCGCCTCCTT CAAGGTCCTG GCCGGGGACA AGAACTTCAT CACTGCTGAG 
GAACTTCGGA GAGAGCTGCC CCCTGACCAG GCTGAGTACT GCATCGCCCG CATGGCGCCC 
TACCAGGGCC CTGATGCTGC TCCAGGCGCC CTTGACTACA AGTCCTTCTC TACAGCCCTC 
TATGGGGAGA GCGACCTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actn4 ( XM_008759185.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_008757407.1 Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ORa43538
Clone ID Related Accession (Same CDS sequence)	XM_008759185.1
Accession Version	XM_008759185.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2802bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	alpha-actinin-4 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005100.4) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGACATCGT GAACACAGCC CGGCCCGACG AGAAGGCCAT AATGACATAT 
GTGTCCAGCT TCTACCATGC CTTTTCAGGA GCGCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGGAC 
CATGGCGGGG CACTGGGGCC TGAGGAATTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC 
GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CAGATGACTT CAGGGCTCTG 
CTTATCTCCA CAGGATACAG CCTGGGTGAT GCTGAGTTCA ACCGGATCAT GAGTGTGGTC 
GACCCCAACC ATAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGACTTCAT GTCAAGGGAG 
ACCACAGACA CAGACACCGC CGATCAGGTC ATCGCCTCCT TCAAGGTCCT GGCCGGGGAC 
AAGAACTTCA TCACTGCTGA GGAACTTCGG AGAGAGCTGC CCCCTGACCA GGCTGAGTAC 
TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGATGCTG CTCCAGGCGC CCTTGACTAC 
AAGTCCTTCT CTACAGCCCT CTATGGGGAG AGCGACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_008757407.1
CDS	148..2949
Translation	MVDYHAANQAYQYGPSSGGNGTGGGGGMGDYMAQEDDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNAKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAEKYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYERLASDLLEWIRRTIPWLEDRVPQKTIQEMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGRMVSDINNGWQHLEQAEKGYEEWLLNEIRRLERLDHLAEKFRQKASIHEAWTDGKEAMLKHRDYETATLSDIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSHNVNTRCQKICDQWDNLGSLTHSRREALEKTEKQLETIDQLHLEYAKRAAPFNNWMESAMEDLQDMFIVHTIEEIEGLISAHDQFKSTLPDADREREAILAIHKEAQRIAESNHIKLSGSNPYTSVTPQIINSKWEKVQQLVPKRDHALLEEQSKQQSNEHLRRQFASQANMVGPWIQTKMEEIGRISIEMNGTLEDQLSHLKQYERSIVDYKPNLDLLEQQHQLIQEALIFDNKHTNYTMEHLRVGWEQLLTTIARTINEVENQILTRDAKGISQEQMQEFRASFNHFDKDHGGALGPEEFKACLISLGYDVENDRQKQTGSMDSDDFRALLISTGYSLGDAEFNRIMSVVDPNHSGLVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNFITAEELRRELPPDQAEYCIARMAPYQGPDAAPGALDYKSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_008759185.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actinin alpha 4 (Actn4), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_008759185.1

ORF Insert Sequence:

ATGGTGGACT ATCACGCGGC GAACCAGGCG TACCAGTACG GCCCAAGCAG CGGCGGCAAT 
GGCACGGGCG GCGGCGGAGG CATGGGCGAC TACATGGCCC AGGAGGATGA CTGGGACCGG 
GACCTGCTGT TGGACCCGGC CTGGGAGAAG CAGCAGCGCA AGACCTTCAC AGCCTGGTGC 
AACTCCCATC TGCGGAAGGC TGGCACTCAG ATCGAGAACA TCGATGAGGA TTTCCGGGAT 
GGGCTCAAAC TTATGCTTCT GCTGGAGGTC ATTTCAGGGG AGCGGTTGCC TAAGCCAGAG 
CGGGGGAAGA TGCGAGTGCA CAAGATCAAC AATGTAAACA AAGCCCTGGA CTTCATCGCC 
AGCAAGGGAG TCAAGCTGGT GTCCATTGGG GCAGAAGAGA TCGTGGATGG CAATGCAAAG 
ATGACCCTGG GAATGATCTG GACCATCATC CTCAGGTTCG CCATCCAAGA CATCTCTGTG 
GAAGAGACCT CTGCCAAGGA AGGACTCCTC CTCTGGTGCC AGAGAAAGAC AGCCCCGTAT 
AAAAACGTCA ACGTCCAGAA CTTCCATATC AGCTGGAAGG ATGGTCTGGC CTTCAACGCG 
CTCATCCACC GGCACAGACC TGAGCTGATT GAGTATGACA AACTGAGGAA GGATGATCCA 
GTCACCAACC TAAACAATGC ATTTGAAGTG GCTGAGAAAT ACCTTGATAT CCCCAAGATG 
TTGGATGCTG AGGACATCGT GAACACAGCC CGGCCCGACG AGAAGGCCAT AATGACATAT 
GTGTCCAGCT TCTACCATGC CTTTTCAGGA GCGCAGAAGG CTGAGACTGC TGCCAACCGG 
ATCTGCAAGG TGCTGGCTGT GAACCAGGAA AATGAGCACC TGATGGAAGA TTACGAGCGC 
CTGGCCAGTG ATCTTCTAGA GTGGATCCGG CGCACCATCC CCTGGCTGGA AGACCGTGTG 
CCTCAGAAGA CCATCCAGGA GATGCAGCAG AAGCTGGAGG ACTTCCGAGA CTATAGGCGT 
GTGCACAAGC CCCCCAAGGT ACAGGAGAAG TGTCAGCTGG AGATCAACTT CAACACCCTG 
CAGACCAAGC TGCGGCTGAG CAACCGGCCT GCCTTCATGC CCTCCGAGGG CAGGATGGTC 
TCGGACATCA ACAATGGGTG GCAACATCTG GAGCAGGCTG AGAAGGGCTA TGAAGAATGG 
CTCTTGAATG AAATCCGTAG GCTGGAACGA CTTGATCACC TGGCAGAGAA ATTCCGGCAG 
AAGGCTTCCA TCCATGAGGC CTGGACCGAT GGGAAGGAGG CCATGCTGAA GCACCGGGAC 
TATGAGACAG CCACCCTGTC AGACATCAAA GCTCTGATCC GAAAGCATGA GGCCTTTGAG 
AGTGACCTGG CGGCGCACCA GGACCGCGTG GAGCAGATCG CGGCTATTGC CCAGGAGCTC 
AACGAACTGG ACTACTATGA CTCCCACAAC GTCAACACAC GGTGCCAGAA GATCTGTGAC 
CAGTGGGATA ACCTGGGCTC TCTGACCCAC AGTCGCAGGG AAGCCTTGGA GAAAACAGAG 
AAACAGCTGG AGACCATCGA CCAGCTACAC TTGGAGTATG CTAAGCGGGC CGCGCCCTTC 
AACAACTGGA TGGAGAGTGC CATGGAGGAC CTGCAGGACA TGTTCATCGT CCACACCATC 
GAGGAGATTG AGGGCCTGAT CTCAGCCCAC GACCAGTTCA AGTCCACCCT GCCAGATGCC 
GACAGGGAGC GTGAGGCCAT CCTGGCCATC CACAAAGAGG CCCAAAGGAT CGCTGAGAGC 
AATCACATCA AGCTGTCGGG CAGCAACCCC TACACCAGCG TTACACCCCA GATCATCAAC 
TCCAAATGGG AGAAGGTGCA GCAGCTGGTG CCGAAGCGGG ACCACGCACT CCTGGAAGAG 
CAGAGCAAGC AGCAATCCAA TGAGCACCTT CGCCGACAGT TTGCCAGCCA AGCCAATATG 
GTGGGGCCCT GGATCCAGAC CAAGATGGAG GAGATTGGGC GCATCTCCAT CGAGATGAAC 
GGGACTCTGG AAGACCAGCT GAGTCACTTG AAGCAGTACG AGCGCAGCAT TGTGGACTAC 
AAGCCCAACC TGGACTTGCT GGAGCAGCAG CACCAGCTCA TCCAGGAGGC CCTCATCTTC 
GACAACAAGC ACACCAACTA CACAATGGAG CACCTCCGTG TGGGCTGGGA GCAGCTGCTC 
ACCACCATTG CCCGCACCAT CAACGAGGTA GAGAATCAGA TCCTCACCCG AGATGCCAAG 
GGCATCAGCC AGGAGCAAAT GCAGGAGTTC CGAGCCTCCT TCAACCACTT TGACAAGGAC 
CATGGCGGGG CACTGGGGCC TGAGGAATTC AAGGCCTGCC TCATCAGCCT GGGCTACGAC 
GTGGAGAATG ACCGGCAGAA GCAGACAGGC AGCATGGACT CAGATGACTT CAGGGCTCTG 
CTTATCTCCA CAGGATACAG CCTGGGTGAT GCTGAGTTCA ACCGGATCAT GAGTGTGGTC 
GACCCCAACC ATAGTGGCCT CGTGACCTTC CAAGCCTTCA TTGACTTCAT GTCAAGGGAG 
ACCACAGACA CAGACACCGC CGATCAGGTC ATCGCCTCCT TCAAGGTCCT GGCCGGGGAC 
AAGAACTTCA TCACTGCTGA GGAACTTCGG AGAGAGCTGC CCCCTGACCA GGCTGAGTAC 
TGCATCGCCC GCATGGCGCC CTACCAGGGC CCTGATGCTG CTCCAGGCGC CCTTGACTAC 
AAGTCCTTCT CTACAGCCCT CTATGGGGAG AGCGACCTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Poly-L-ornithine enhances migration of neural stem/progenitor cells via promoting ?-Actinin 4 binding to actin filaments.
Scientific reports637681(2016 Nov)
Ge H,Yu A,Chen J,Yuan J,Yin Y,Duanmu W,Tan L,Yang Y,Lan C,Chen W,Feng H,Hu R

A complex of Rab13 with MICAL-L2 and ?-actinin-4 is essential for insulin-dependent GLUT4 exocytosis.
Molecular biology of the cell27(1)75-89(2016 Jan)
Sun Y,Jaldin-Fincati J,Liu Z,Bilan PJ,Klip A

Alpha-actinin binding kinetics modulate cellular dynamics and force generation.
Proceedings of the National Academy of Sciences of the United States of America112(21)6619-24(2015 May)
Ehrlicher AJ,Krishnan R,Guo M,Bidan CM,Weitz DA,Pollak MR

?-Actinin 4 potentiates nuclear factor ?-light-chain-enhancer of activated B-cell (NF-?B) activity in podocytes independent of its cytoplasmic actin binding function.
The Journal of biological chemistry290(1)338-49(2015 Jan)
Zhao X,Hsu KS,Lim JH,Bruggeman LA,Kao HY

Possible role of non-muscle alpha-actinins in muscle cell mechanosensitivity.
PloS one9(4)e96395(2014)
Ogneva IV,Biryukov NS,Leinsoo TA,Larina IM

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オンライン注文

Actn4 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

見積依頼

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