actn4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene
Clones
gRNAs

Summary

Gene Symbol	actn4
Entrez Gene ID	448522
Full Name	actinin alpha 4
Synonyms	fsgs,fsgs1
General protein information	Preferred Names actinin alpha 4 Names alpha-actinin-4
Gene Type	protein-coding
Organism	Xenopus tropicalis(tropical clawed frog)

mRNA and Protein(s)

mRNA	Protein	Name
XM_018096215.1	XP_017951704.1	alpha-actinin-4 isoform X1
XM_018096217.2	XP_017951706.1	alpha-actinin-4 isoform X3
XM_018096217.1	XP_017951706.1	alpha-actinin-4 isoform X3
XM_018096216.1	XP_017951705.1	alpha-actinin-4 isoform X2
NM_001006809.1	NP_001006810.1	alpha-actinin-4
XM_018096215.2	XP_017951704.1	alpha-actinin-4 isoform X1
XM_018096216.2	XP_017951705.1	alpha-actinin-4 isoform X2

Pathways from BioSystems

Homologies

	ACTN4	XP_001083825.1
Danio rerio (zebrafish)	actn4	NP_955880.1
Pan troglodytes (chimpanzee)	ACTN4	XP_003954421.1
Rattus norvegicus (Norway rat)	Actn4	NP_113863.2
Homo sapiens (human)	ACTN4	NP_004915.2
Bos taurus (cattle)	ACTN4	NP_001091521.1
Schizosaccharomyces pombe (fission yeast)	ain1	NP_594295.1
Canis lupus familiaris (dog)	ACTN4	XP_541640.2
Mus musculus (house mouse)	Actn4	NP_068695.1
Gallus gallus (chicken)	ACTN4	NP_990457.1
Magnaporthe oryzae (rice blast fungus)	MGG_06475	XP_003717088.1
Neurospora crassa	NCU06429	XP_957320.1
Xenopus tropicalis (tropical clawed frog)	actn4	NP_001006810.1

Gene Ontology
Bibliography

Related articles in PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Klein SL, Strausberg RL, Wagner L, Pontius J, Clifton SW, Richardson P
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following actn4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the actn4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OXa21500	NM_001006809.1 Latest version!	Xenopus tropicalis actinin alpha 4 (actn4), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OXa46003	XM_018096215.1 Latest version!	Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa46004	XM_018096216.1 Latest version!	Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa46005	XM_018096217.1 Latest version!	Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
OXa63616	XM_018096215.2 Latest version!	Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa63617	XM_018096216.2 Latest version!	Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OXa63618	XM_018096217.2 Latest version!	Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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5 or more Clone	50% OFF
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*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

actn4 ( NM_001006809.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> NP_001006810.1 Xenopus tropicalis actinin alpha 4 (actn4), mRNA.

Price & Availability↑

CloneID	OXa21500
Clone ID Related Accession (Same CDS sequence)	NM_001006809.1
Accession Version	NM_001006809.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-4
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC076687.1. ##Evidence-Data-START## Transcript exon combination :: BC076687.1, CR926289.2 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMD00028290, SAMD00028291 [ECO:0000350] ##Evidence-Data-END##

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATTATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGACATTGTC 
AACACTGCTC GTCCTGATGA GAAGGCAATC ATGACATACG TGTCCTCTTT CTACCACGCC 
TTCTCTGGAG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGGGA 
GATGCAGAGT TTAACCGGAT TATGGCTATC GTTGACCCGA ACAACACTGG CATGGTTACT 
TTCCAAGCAT TCATAGACTT TATGTCCCGA GAGACAACAG ACACAGACAC AGCTGACCAA 
GTCATAGCCT CATTTAAGGT CCTGGCTGGA GATAAAAATT ACATCACTGC AGCAGAGCTG 
CGTAGAGAGC TTCCTCCAGA TCAGGCAGAA TATTGCATTG CTCGTATGGC ACCCTACCAG 
GGACCTGACG CAGTGCCTGG AGCACTGGAT TACATGTCTT TCTCTACTGC TTTGTATGGG 
GAGAGTGACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001006810.1
CDS	172..2886
Translation	MVDYHMTSGGAPPHMGQLGQDQRYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNTKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRVPEKTMHDMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGFEEWLLNAIRRLERLDHLAEKFRQKALIHEAWTEGKEAMLKQKDYESATLADIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDLLGSLTHTRREALEVTEKQLEAIDQLYLEYAKRAAPFNNWMEGAMEDLQDMFIVHTIEEIEGLIAAHEQFKSTLPDADREREAILGIQNEAQKIADYSHIKLASGNPYTTVTPQIINSKWEKVQQLVPKRDGALKGEHDKQQSNEHLRLQFASLANVVGPWIQAKMEEIGRIAIELHGTLEDQMTHLKQYEQSVINYKPNIDKLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEHLLTTIARTSNEVENQILTRDAKGISQEQMHEYRASFNHFDRDHTGALGPEEFKACLISLGYDVENDRQGDAEFNRIMAIVDPNNTGMVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNYITAAELRRELPPDQAEYCIARMAPYQGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	NM_001006809.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin alpha 4 (actn4), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001006809.1

ORF Insert Sequence:

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATTATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGACATTGTC 
AACACTGCTC GTCCTGATGA GAAGGCAATC ATGACATACG TGTCCTCTTT CTACCACGCC 
TTCTCTGGAG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGGGA 
GATGCAGAGT TTAACCGGAT TATGGCTATC GTTGACCCGA ACAACACTGG CATGGTTACT 
TTCCAAGCAT TCATAGACTT TATGTCCCGA GAGACAACAG ACACAGACAC AGCTGACCAA 
GTCATAGCCT CATTTAAGGT CCTGGCTGGA GATAAAAATT ACATCACTGC AGCAGAGCTG 
CGTAGAGAGC TTCCTCCAGA TCAGGCAGAA TATTGCATTG CTCGTATGGC ACCCTACCAG 
GGACCTGACG CAGTGCCTGG AGCACTGGAT TACATGTCTT TCTCTACTGC TTTGTATGGG 
GAGAGTGACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_018096215.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951704.1 Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa46003
Clone ID Related Accession (Same CDS sequence)	XM_018096215.1
Accession Version	XM_018096215.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA AGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGGGA 
GATGCAGAGT TTAACCGGAT TATGGCTATC GTTGACCCGA ACAACACTGG CATGGTTACT 
TTCCAAGCAT TCATAGACTT TATGTCCCGA GAGACAACAG ACACAGACAC AGCTGACCAA 
GTCATAGCCT CATTTAAGGT CCTGGCTGGA GATAAAAATT ACATCACTGC AGCAGAGCTG 
CGTAGAGAGC TTCCTCCAGA TCAGGCAGAA TATTGCATTG CTCGTATGGC ACCCTACCAG 
GGACCTGACG CAGTGCCTGG AGCACTGGAT TACATGTCTT TCTCTACTGC TTTGTATGGG 
GAGAGTGACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951704.1
CDS	344..3058
Translation	MVDYHMTSGGAPPHMGQLGQDQRYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNTKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRVPEKTMHDMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGFEEWLLNAIRRLERLDHLAEKFRQKALIHEAWTEGKEAMLKQKDYESATLADIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDLLGSLTHTRREALEVTEKQLEAIDQLYLEYAKRAAPFNNWMEGAMEDLQDMFIVHTIEEIEGLIAAHEQFKSTLPDADREREAILGIQNEAQKIADYSHIKLASGNPYTTVTPQIINSKWEKVQQLVPKRDGALKGEHDKQQSNEHLRLQFASLANVVGPWIQAKMEEIGRIAIELHGTLEDQMTHLKQYEQSVINYKPNIDKLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEHLLTTIARTSNEVENQILTRDAKGISQEQMHEYRASFNHFDRDHTGALGPEEFKACLISLGYDVENDRQGDAEFNRIMAIVDPNNTGMVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNYITAAELRRELPPDQAEYCIARMAPYQGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_018096215.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096215.1

ORF Insert Sequence:

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA AGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGGGA 
GATGCAGAGT TTAACCGGAT TATGGCTATC GTTGACCCGA ACAACACTGG CATGGTTACT 
TTCCAAGCAT TCATAGACTT TATGTCCCGA GAGACAACAG ACACAGACAC AGCTGACCAA 
GTCATAGCCT CATTTAAGGT CCTGGCTGGA GATAAAAATT ACATCACTGC AGCAGAGCTG 
CGTAGAGAGC TTCCTCCAGA TCAGGCAGAA TATTGCATTG CTCGTATGGC ACCCTACCAG 
GGACCTGACG CAGTGCCTGG AGCACTGGAT TACATGTCTT TCTCTACTGC TTTGTATGGG 
GAGAGTGACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_018096216.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951705.1 Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa46004
Clone ID Related Accession (Same CDS sequence)	XM_018096216.1
Accession Version	XM_018096216.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA AGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGACATTGTC 
AACACTGCTC GTCCTGATGA GAAGGCAATC ATGACATACG TGTCCTCTTT CTACCACGCC 
TTCTCTGGAG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGAAG 
AGAATCGGTG CACTGGATGC TGATGACTTT CGTGCTCTCC TTATCTCTAC TGGATACAGC 
TTGGGAGATG CAGAGTTTAA CCGGATTATG GCTATCGTTG ACCCGAACAA CACTGGCATG 
GTTACTTTCC AAGCATTCAT AGACTTTATG TCCCGAGAGA CAACAGACAC AGACACAGCT 
GACCAAGTCA TAGCCTCATT TAAGGTCCTG GCTGGAGATA AAAATTACAT CACTGCAGCA 
GAGCTGCGTA GAGAGCTTCC TCCAGATCAG GCAGAATATT GCATTGCTCG TATGGCACCC 
TACCAGGGAC CTGACGCAGT GCCTGGAGCA CTGGATTACA TGTCTTTCTC TACTGCTTTG 
TATGGGGAGA GTGACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951705.1
CDS	344..3124
Translation	MVDYHMTSGGAPPHMGQLGQDQRYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNTKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRVPEKTMHDMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGFEEWLLNAIRRLERLDHLAEKFRQKALIHEAWTEGKEAMLKQKDYESATLADIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDLLGSLTHTRREALEVTEKQLEAIDQLYLEYAKRAAPFNNWMEGAMEDLQDMFIVHTIEEIEGLIAAHEQFKSTLPDADREREAILGIQNEAQKIADYSHIKLASGNPYTTVTPQIINSKWEKVQQLVPKRDGALKGEHDKQQSNEHLRLQFASLANVVGPWIQAKMEEIGRIAIELHGTLEDQMTHLKQYEQSVINYKPNIDKLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEHLLTTIARTSNEVENQILTRDAKGISQEQMHEYRASFNHFDRDHTGALGPEEFKACLISLGYDVENDRQKRIGALDADDFRALLISTGYSLGDAEFNRIMAIVDPNNTGMVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNYITAAELRRELPPDQAEYCIARMAPYQGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_018096216.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096216.1

ORF Insert Sequence:

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA AGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGACATTGTC 
AACACTGCTC GTCCTGATGA GAAGGCAATC ATGACATACG TGTCCTCTTT CTACCACGCC 
TTCTCTGGAG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGAAG 
AGAATCGGTG CACTGGATGC TGATGACTTT CGTGCTCTCC TTATCTCTAC TGGATACAGC 
TTGGGAGATG CAGAGTTTAA CCGGATTATG GCTATCGTTG ACCCGAACAA CACTGGCATG 
GTTACTTTCC AAGCATTCAT AGACTTTATG TCCCGAGAGA CAACAGACAC AGACACAGCT 
GACCAAGTCA TAGCCTCATT TAAGGTCCTG GCTGGAGATA AAAATTACAT CACTGCAGCA 
GAGCTGCGTA GAGAGCTTCC TCCAGATCAG GCAGAATATT GCATTGCTCG TATGGCACCC 
TACCAGGGAC CTGACGCAGT GCCTGGAGCA CTGGATTACA TGTCTTTCTC TACTGCTTTG 
TATGGGGAGA GTGACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_018096217.1 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951706.1 Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OXa46005
Clone ID Related Accession (Same CDS sequence)	XM_018096217.1
Accession Version	XM_018096217.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-09-07
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Xenopus tropicalis Annotation Release 103 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA AGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGAAGA GAATCGGTGC ACTGGATGCT 
GATGACTTTC GTGCTCTCCT TATCTCTACT GGATACAGCT TGGGAGATGC AGAGTTTAAC 
CGGATTATGG CTATCGTTGA CCCGAACAAC ACTGGCATGG TTACTTTCCA AGCATTCATA 
GACTTTATGT CCCGAGAGAC AACAGACACA GACACAGCTG ACCAAGTCAT AGCCTCATTT 
AAGGTCCTGG CTGGAGATAA AAATTACATC ACTGCAGCAG AGCTGCGTAG AGAGCTTCCT 
CCAGATCAGG CAGAATATTG CATTGCTCGT ATGGCACCCT ACCAGGGACC TGACGCAGTG 
CCTGGAGCAC TGGATTACAT GTCTTTCTCT ACTGCTTTGT ATGGGGAGAG TGACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951706.1
CDS	344..3043
Translation	MVDYHMTSGGAPPHMGQLGQDQRYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNTKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRVPEKTMHDMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGFEEWLLNAIRRLERLDHLAEKFRQKALIHEAWTEGKEAMLKQKDYESATLADIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDLLGSLTHTRREALEVTEKQLEAIDQLYLEYAKRAAPFNNWMEGAMEDLQDMFIVHTIEEIEGLIAAHEQFKSTLPDADREREAILGIQNEAQKIADYSHIKLASGNPYTTVTPQIINSKWEKVQQLVPKRDGALKGEHDKQQSNEHLRLQFASLANVVGPWIQAKMEEIGRIAIELHGTLEDQMTHLKQYEQSVINYKPNIDKLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEHLLTTIARTSNEVENQILTRDAKGISQEQMHEYRASFNHFDRKRIGALDADDFRALLISTGYSLGDAEFNRIMAIVDPNNTGMVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNYITAAELRRELPPDQAEYCIARMAPYQGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_018096217.1
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin, alpha 4 (actn4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096217.1

ORF Insert Sequence:

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA AGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGAAGA GAATCGGTGC ACTGGATGCT 
GATGACTTTC GTGCTCTCCT TATCTCTACT GGATACAGCT TGGGAGATGC AGAGTTTAAC 
CGGATTATGG CTATCGTTGA CCCGAACAAC ACTGGCATGG TTACTTTCCA AGCATTCATA 
GACTTTATGT CCCGAGAGAC AACAGACACA GACACAGCTG ACCAAGTCAT AGCCTCATTT 
AAGGTCCTGG CTGGAGATAA AAATTACATC ACTGCAGCAG AGCTGCGTAG AGAGCTTCCT 
CCAGATCAGG CAGAATATTG CATTGCTCGT ATGGCACCCT ACCAGGGACC TGACGCAGTG 
CCTGGAGCAC TGGATTACAT GTCTTTCTCT ACTGCTTTGT ATGGGGAGAG TGACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_018096215.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951704.1 Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OXa63616
Clone ID Related Accession (Same CDS sequence)	XM_018096215.2
Accession Version	XM_018096215.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2715bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-4 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096215.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGGGA 
GATGCAGAGT TTAACCGGAT TATGGCTATC GTTGACCCGA ACAACACTGG CATGGTTACT 
TTCCAAGCAT TCATAGACTT TATGTCCCGA GAGACAACAG ACACAGACAC AGCTGACCAA 
GTCATAGCCT CATTTAAGGT CCTGGCTGGA GATAAAAATT ACATCACTGC AGCAGAGCTG 
CGTAGAGAGC TTCCTCCAGA TCAGGCAGAA TATTGCATTG CTCGTATGGC ACCCTACCAG 
GGACCTGACG CAGTGCCTGG AGCACTGGAT TACATGTCTT TCTCTACTGC TTTGTATGGG 
GAGAGTGACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951704.1
CDS	236..2950
Translation	MVDYHMTSGGAPPHMGQLGQDQRYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNTKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRVPEKTMHDMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGFEEWLLNAIRRLERLDHLAEKFRQKALIHEAWTEGKEAMLKQKDYESATLADIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDLLGSLTHTRREALEVTEKQLEAIDQLYLEYAKRAAPFNNWMEGAMEDLQDMFIVHTIEEIEGLIAAHEQFKSTLPDADREREAILGIQNEAQKIADYSHIKLASGNPYTTVTPQIINSKWEKVQQLVPKRDGALKGEHDKQQSNEHLRLQFASLANVVGPWIQAKMEEIGRIAIELHGTLEDQMTHLKQYEQSVINYKPNIDKLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEHLLTTIARTSNEVENQILTRDAKGISQEQMHEYRASFNHFDRDHTGALGPEEFKACLISLGYDVENDRQGDAEFNRIMAIVDPNNTGMVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNYITAAELRRELPPDQAEYCIARMAPYQGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_018096215.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096215.2

ORF Insert Sequence:

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGGGA 
GATGCAGAGT TTAACCGGAT TATGGCTATC GTTGACCCGA ACAACACTGG CATGGTTACT 
TTCCAAGCAT TCATAGACTT TATGTCCCGA GAGACAACAG ACACAGACAC AGCTGACCAA 
GTCATAGCCT CATTTAAGGT CCTGGCTGGA GATAAAAATT ACATCACTGC AGCAGAGCTG 
CGTAGAGAGC TTCCTCCAGA TCAGGCAGAA TATTGCATTG CTCGTATGGC ACCCTACCAG 
GGACCTGACG CAGTGCCTGG AGCACTGGAT TACATGTCTT TCTCTACTGC TTTGTATGGG 
GAGAGTGACT TGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_018096216.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951705.1 Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OXa63617
Clone ID Related Accession (Same CDS sequence)	XM_018096216.2
Accession Version	XM_018096216.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2781bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-4 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096216.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGACATTGTC 
AACACTGCTC GTCCTGATGA GAAGGCAATC ATGACATACG TGTCCTCTTT CTACCACGCC 
TTCTCTGGAG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGAAG 
AGAATCGGTG CACTGGATGC TGATGACTTT CGTGCTCTCC TTATCTCTAC TGGATACAGC 
TTGGGAGATG CAGAGTTTAA CCGGATTATG GCTATCGTTG ACCCGAACAA CACTGGCATG 
GTTACTTTCC AAGCATTCAT AGACTTTATG TCCCGAGAGA CAACAGACAC AGACACAGCT 
GACCAAGTCA TAGCCTCATT TAAGGTCCTG GCTGGAGATA AAAATTACAT CACTGCAGCA 
GAGCTGCGTA GAGAGCTTCC TCCAGATCAG GCAGAATATT GCATTGCTCG TATGGCACCC 
TACCAGGGAC CTGACGCAGT GCCTGGAGCA CTGGATTACA TGTCTTTCTC TACTGCTTTG 
TATGGGGAGA GTGACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951705.1
CDS	237..3017
Translation	MVDYHMTSGGAPPHMGQLGQDQRYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNTKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVNTARPDEKAIMTYVSSFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRVPEKTMHDMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGFEEWLLNAIRRLERLDHLAEKFRQKALIHEAWTEGKEAMLKQKDYESATLADIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDLLGSLTHTRREALEVTEKQLEAIDQLYLEYAKRAAPFNNWMEGAMEDLQDMFIVHTIEEIEGLIAAHEQFKSTLPDADREREAILGIQNEAQKIADYSHIKLASGNPYTTVTPQIINSKWEKVQQLVPKRDGALKGEHDKQQSNEHLRLQFASLANVVGPWIQAKMEEIGRIAIELHGTLEDQMTHLKQYEQSVINYKPNIDKLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEHLLTTIARTSNEVENQILTRDAKGISQEQMHEYRASFNHFDRDHTGALGPEEFKACLISLGYDVENDRQKRIGALDADDFRALLISTGYSLGDAEFNRIMAIVDPNNTGMVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNYITAAELRRELPPDQAEYCIARMAPYQGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_018096216.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096216.2

ORF Insert Sequence:

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGACATTGTC 
AACACTGCTC GTCCTGATGA GAAGGCAATC ATGACATACG TGTCCTCTTT CTACCACGCC 
TTCTCTGGAG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGGATC ATACAGGTGC ATTGGGACCT 
GAAGAATTCA AAGCATGTCT GATCAGTCTG GGATATGATG TGGAAAATGA CAGACAGAAG 
AGAATCGGTG CACTGGATGC TGATGACTTT CGTGCTCTCC TTATCTCTAC TGGATACAGC 
TTGGGAGATG CAGAGTTTAA CCGGATTATG GCTATCGTTG ACCCGAACAA CACTGGCATG 
GTTACTTTCC AAGCATTCAT AGACTTTATG TCCCGAGAGA CAACAGACAC AGACACAGCT 
GACCAAGTCA TAGCCTCATT TAAGGTCCTG GCTGGAGATA AAAATTACAT CACTGCAGCA 
GAGCTGCGTA GAGAGCTTCC TCCAGATCAG GCAGAATATT GCATTGCTCG TATGGCACCC 
TACCAGGGAC CTGACGCAGT GCCTGGAGCA CTGGATTACA TGTCTTTCTC TACTGCTTTG 
TATGGGGAGA GTGACTTGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

actn4 ( XM_018096217.2 ) cDNA ORF clone, Xenopus tropicalis(tropical clawed frog) -> XP_017951706.1 Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X3, mRNA.

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CloneID	OXa63618
Clone ID Related Accession (Same CDS sequence)	XM_018096217.2
Accession Version	XM_018096217.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-17
Organism	Xenopus tropicalis(tropical clawed frog)
Product	alpha-actinin-4 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_030684.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Dec 18, 2019 this sequence version replaced XM_018096217.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Xenopus tropicalis Annotation Release 104 Annotation Version :: 104 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGAAGA GAATCGGTGC ACTGGATGCT 
GATGACTTTC GTGCTCTCCT TATCTCTACT GGATACAGCT TGGGAGATGC AGAGTTTAAC 
CGGATTATGG CTATCGTTGA CCCGAACAAC ACTGGCATGG TTACTTTCCA AGCATTCATA 
GACTTTATGT CCCGAGAGAC AACAGACACA GACACAGCTG ACCAAGTCAT AGCCTCATTT 
AAGGTCCTGG CTGGAGATAA AAATTACATC ACTGCAGCAG AGCTGCGTAG AGAGCTTCCT 
CCAGATCAGG CAGAATATTG CATTGCTCGT ATGGCACCCT ACCAGGGACC TGACGCAGTG 
CCTGGAGCAC TGGATTACAT GTCTTTCTCT ACTGCTTTGT ATGGGGAGAG TGACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017951706.1
CDS	241..2940
Translation	MVDYHMTSGGAPPHMGQLGQDQRYMEQEEDWDRDLLLDPAWEKQQRKTFTAWCNSHLRKAGTQIENIDEDFRDGLKLMLLLEVISGERLPKPERGKMRVHKINNVNKALDFIASKGVKLVSIGAEEIVDGNTKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNVQNFHISWKDGLAFNALIHRHRPELIEYDKLRKDDPVTNLNNAFEVAERYLDIPKMLDAEDIVGTLRPDEKAIMTYVSCFYHAFSGAQKAETAANRICKVLAVNQENEHLMEDYEKLASDLLEWIRRTIPWLENRVPEKTMHDMQQKLEDFRDYRRVHKPPKVQEKCQLEINFNTLQTKLRLSNRPAFMPSEGKMVSDINNGWQHLEQAEKGFEEWLLNAIRRLERLDHLAEKFRQKALIHEAWTEGKEAMLKQKDYESATLADIKALIRKHEAFESDLAAHQDRVEQIAAIAQELNELDYYDSPSVNARCQKICDQWDLLGSLTHTRREALEVTEKQLEAIDQLYLEYAKRAAPFNNWMEGAMEDLQDMFIVHTIEEIEGLIAAHEQFKSTLPDADREREAILGIQNEAQKIADYSHIKLASGNPYTTVTPQIINSKWEKVQQLVPKRDGALKGEHDKQQSNEHLRLQFASLANVVGPWIQAKMEEIGRIAIELHGTLEDQMTHLKQYEQSVINYKPNIDKLEQQHQLIQEALIFDNKHTNYTMEHIRVGWEHLLTTIARTSNEVENQILTRDAKGISQEQMHEYRASFNHFDRKRIGALDADDFRALLISTGYSLGDAEFNRIMAIVDPNNTGMVTFQAFIDFMSRETTDTDTADQVIASFKVLAGDKNYITAAELRRELPPDQAEYCIARMAPYQGPDAVPGALDYMSFSTALYGESDL

Target ORF information:

RefSeq Version	XM_018096217.2
Organism	Xenopus tropicalis(tropical clawed frog)
Definition	Xenopus tropicalis actinin alpha 4 (actn4), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_018096217.2

ORF Insert Sequence:

ATGGTGGATT ATCACATGAC CAGCGGAGGG GCGCCCCCTC ACATGGGCCA GCTGGGACAA 
GACCAACGGT ATATGGAACA GGAGGAGGAC TGGGATCGGG ACCTGTTGTT GGACCCAGCA 
TGGGAAAAGC AGCAGAGGAA GACTTTTACA GCATGGTGCA ACTCCCATCT AAGGAAGGCC 
GGCACACAAA TAGAAAATAT TGATGAAGAT TTCAGAGATG GGTTGAAGCT CATGTTACTA 
CTGGAAGTCA TCTCAGGAGA ACGGCTGCCC AAACCAGAGC GAGGCAAGAT GAGGGTGCAT 
AAAATCAATA ATGTCAATAA AGCCCTGGAT TTCATTGCAA GTAAAGGAGT GAAGTTGGTT 
TCCATTGGAG CAGAAGAAAT TGTTGATGGC AATACAAAAA TGACATTAGG AATGATCTGG 
ACAATCATCC TGCGGTTTGC CATTCAGGAC ATATCTGTAG AAGAAACCTC TGCAAAGGAA 
GGACTTCTTT TGTGGTGCCA GAGGAAGACT GCCCCTTATA AGAATGTCAA TGTTCAGAAC 
TTCCACATCA GCTGGAAGGA TGGGCTGGCT TTCAATGCCC TAATCCATAG ACATCGCCCA 
GAACTGATAG AGTATGACAA GCTGAGAAAG GATGACCCAG TTACTAACCT AAACAATGCT 
TTTGAAGTAG CAGAAAGATA TCTTGACATT CCCAAGATGT TGGATGCTGA AGATATTGTG 
GGCACTCTCA GGCCTGATGA GAAGGCCATA ATGACCTACG TTTCCTGCTT CTACCACGCT 
TTCTCTGGTG CTCAGAAGGC TGAGACAGCA GCTAACAGAA TTTGTAAGGT GTTGGCTGTA 
AATCAAGAAA ATGAGCATCT AATGGAGGAC TATGAAAAGC TGGCTAGTGA TCTTTTGGAG 
TGGATCCGTC GTACAATCCC TTGGTTGGAG AATCGTGTAC CTGAAAAAAC TATGCATGAC 
ATGCAGCAAA AGCTGGAAGA TTTCCGAGAT TACCGTCGTG TGCACAAACC ACCTAAAGTG 
CAAGAGAAGT GCCAATTAGA AATCAACTTC AACACCCTGC AAACAAAACT GAGGCTCAGC 
AATCGTCCTG CTTTTATGCC ATCGGAGGGC AAGATGGTTT CGGATATTAA TAATGGATGG 
CAACACTTGG AGCAGGCTGA GAAAGGCTTT GAGGAGTGGC TTTTGAATGC AATCAGACGG 
TTGGAGAGAC TGGATCACTT GGCAGAAAAG TTTCGTCAGA AAGCCCTCAT CCACGAGGCC 
TGGACAGAAG GCAAAGAGGC AATGCTGAAG CAGAAAGATT ATGAAAGTGC CACCCTTGCA 
GACATAAAGG CACTTATCCG AAAACACGAA GCATTTGAAA GTGACCTTGC AGCCCACCAG 
GATCGTGTAG AACAAATAGC AGCTATTGCA CAGGAATTAA ATGAGTTGGA TTACTACGAC 
TCCCCCAGCG TCAATGCTCG GTGCCAGAAG ATATGTGACC AGTGGGATCT TCTGGGCTCC 
TTAACACACA CCAGGAGAGA AGCCCTAGAG GTAACGGAGA AACAGTTGGA AGCAATTGAC 
CAGCTGTACC TGGAATATGC TAAGCGAGCT GCACCATTCA ACAACTGGAT GGAAGGAGCT 
ATGGAGGATT TGCAAGACAT GTTCATAGTG CACACCATTG AGGAGATTGA GGGTCTGATT 
GCAGCTCATG AGCAGTTCAA GTCCACTCTT CCGGATGCTG ACCGTGAGAG AGAAGCCATT 
CTTGGGATTC AGAACGAGGC TCAGAAAATT GCGGATTACA GCCACATCAA ACTAGCCAGT 
GGGAATCCTT ATACAACTGT AACTCCGCAG ATTATTAACT CAAAATGGGA GAAGGTTCAG 
CAGCTTGTTC CTAAAAGGGA TGGTGCATTG AAAGGAGAAC ATGACAAGCA GCAGTCCAAT 
GAGCACCTGC GCTTGCAGTT TGCCAGCCTA GCTAATGTGG TAGGACCTTG GATACAGGCC 
AAGATGGAGG AGATTGGCAG GATTGCTATA GAGTTGCACG GTACTCTGGA AGACCAGATG 
ACCCACCTGA AGCAGTATGA ACAGAGTGTC ATAAATTACA AGCCCAATAT TGACAAACTG 
GAGCAGCAGC ATCAGCTCAT CCAGGAAGCC CTGATCTTTG ATAACAAGCA CACTAACTAT 
ACTATGGAGC ACATCCGTGT AGGCTGGGAA CATCTTCTTA CTACAATAGC TCGCACAAGT 
AATGAGGTGG AAAACCAAAT CCTTACCCGT GATGCCAAGG GTATCAGCCA GGAGCAGATG 
CATGAATACC GGGCATCATT CAATCACTTT GATAGGAAGA GAATCGGTGC ACTGGATGCT 
GATGACTTTC GTGCTCTCCT TATCTCTACT GGATACAGCT TGGGAGATGC AGAGTTTAAC 
CGGATTATGG CTATCGTTGA CCCGAACAAC ACTGGCATGG TTACTTTCCA AGCATTCATA 
GACTTTATGT CCCGAGAGAC AACAGACACA GACACAGCTG ACCAAGTCAT AGCCTCATTT 
AAGGTCCTGG CTGGAGATAA AAATTACATC ACTGCAGCAG AGCTGCGTAG AGAGCTTCCT 
CCAGATCAGG CAGAATATTG CATTGCTCGT ATGGCACCCT ACCAGGGACC TGACGCAGTG 
CCTGGAGCAC TGGATTACAT GTCTTTCTCT ACTGCTTTGT ATGGGGAGAG TGACTTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genetic and genomic tools for Xenopus research: The NIH Xenopus initiative.
Developmental dynamics : an official publication of the American Association of Anatomists225(4)384-91(2002 Dec)
Klein SL,Strausberg RL,Wagner L,Pontius J,Clifton SW,Richardson P

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actn4 cDNA ORF clone, Xenopus tropicalis(tropical clawed frog)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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