dna-2 cDNA ORF clone, Caenorhabditis elegans

Gene
Clones
gRNAs

Summary

Gene Symbol	dna-2
Entrez Gene ID	174809
Full Name	yeast DNA helicase/endonuclease family
Gene Type	protein-coding
Organism	Caenorhabditis elegans

mRNA and Protein(s)

mRNA	Protein	Name
NM_064115.3	NP_496516.1	yeast DNA helicase/endonuclease family
NM_064115.4	NP_496516.1	yeast DNA helicase/endonuclease family

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	DNA2	NP_001006497.1
Bos taurus (cattle)	DNA2	NP_001179731.2
Rattus norvegicus (Norway rat)	Dna2	XP_241671.6
Arabidopsis thaliana (thale cress)	emb2411	NP_001184943.1
Oryza sativa (rice)	Os04g0588200	NP_001053694.2
Canis lupus familiaris (dog)	DNA2	XP_005619489.1
Danio rerio (zebrafish)	dna2	XP_687974.5
Pan troglodytes (chimpanzee)	DNA2	XP_001163213.2
Mus musculus (house mouse)	Dna2	NP_796346.2
Drosophila melanogaster (fruit fly)	CG2990	NP_727386.1
Homo sapiens (human)	DNA2	NP_001073918.2
	DNA2	XP_002808547.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP004685	XP_307265.4
Caenorhabditis elegans (roundworm)	dna-2	NP_496515.1
Schizosaccharomyces pombe (fission yeast)	dna2	NP_596499.2
Magnaporthe oryzae (rice blast fungus)	MGG_02491	XP_003709288.1
Xenopus tropicalis (tropical clawed frog)	dna2	XP_002936016.2

Gene Ontology
Bibliography

Related articles in PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.

Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Cedna-2 contributes to embryonic viability, the morphogenesis of both late-stage embryos and male sensory rays, and normal life span.
Title: Caenorhabditis elegans DNA-2 helicase/endonuclease plays a vital role in maintaining genome stability, morphogenesis, and life span.

A comparative study of the enzymatic properties of C.elegans Dna2 and S.cerevisiae Dna2 and their interaction with replication-protein A.
Title: Enzymatic properties of the Caenorhabditis elegans Dna2 endonuclease/helicase and a species-specific interaction between RPA and Dna2.

Dna2 localizes to the nuclei of C. elegans oocytes & early embryos. Homozygous deletion causes temperature-dependent reduced brood size & embryonic lethality, delayed cell-division.
Title: Dna2 requirement for normal reproduction of Caenorhabditis elegans is temperature-dependent.

The following dna-2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dna-2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCa338234	NM_064115.4 Latest version!	Caenorhabditis elegans yeast DNA helicase/endonuclease family (dna-2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
OCa338234	NM_064115.3	Caenorhabditis elegans yeast DNA helicase/endonuclease family (dna-2), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

dna-2 ( NM_064115.3 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496516.1 Caenorhabditis elegans yeast DNA helicase/endonuclease family (dna-2), partial mRNA.

Price & Availability↑

CloneID	OCa338234
Clone ID Related Accession (Same CDS sequence)	NM_064115.3 , NM_064115.4
Accession Version	NM_064115.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-08
Organism	Caenorhabditis elegans
Product	yeast DNA helicase/endonuclease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Jun 26, 2012 this sequence version replaced NM_064115.2. COMPLETENESS: incomplete on both ends.

ATGGAGTCTC GCAAAAGACA GCTCCAAAAT GTCAATTCAG AATCACACGA GCCGAAACGT 
CTAAAAGAGG AATTCAAGGG AACCGAGCAT GATGGAGACA GCGTTGACTC GTTCGAGAAC 
TTCTGTCCGA CAGCTTCCTC TCCAAAAGAA AAAATCAGCC CGAGGTCGAG AAGTGAAAGG 
ATACAACTTC AAGAATGCGA CGATGACGAT TCGTTCGATG CACCAGTCAT TAGTTCAAAA 
CCTCCGGCTT TACCGAAAGA TAGCGGAGTT TCTGGATTCC CATGCCTCTT CGACGATAAA 
AATGTGATAC ATCTAACAGC TCGAAAAGGT GAAAAAAGAG ACGATTGTCT AGATTTGGAG 
TGTATAGACA AAGAAGGGAC GACTAGGATA GTCTATTTAC AGGATCATTG GTCGGAAATG 
AGCATTGAAG CAGGATCCAC GATTAACCTG ATCGGAGCTC AAGCATGGGG AGATCGTGAT 
TTCCTTGTCA ACGATGACGT CGGAATCGTG ATCTTAAATC CAGACGCACT TGTCCCGTGC 
ACAGCCGTCG CTTCAGCCAC ATTTTGTGCT CGAAAAACGG TGCTAAACGA CAAGTTTAAG 
TTTGGAAATG CCTCGAACAA AGCAATGCTT CTCGGAACCA TTATGCACGA GATATTCCAG 
ACAGCAATCA CGAGCAAAAA GCGACCTGTA ATGGAAAACG ATTTGATGAA AATTTGGAAC 
AAGCAGGCTC CAAAGTATGC CGAAGAGCTT GTGGCGCTCT CCTTTACTCC AAGCTGTCTG 
GACGCCGAGC TACAGCCTTA CTTTTCGATA ATCTGTGGCT GGATCAATAA ACATTATCCA 
CTTTCTAACA GCTTCTTCTC GAAACGCGAG CCTCTGCCGT CAAAATCGGA GCTTCTCGAG 
GTGTACGATA TTGAGGAGAA TATATGGGAT TCAAAGCTTG GTTTGAAGGG GAAGATCGAT 
GTGACGATGC GAACAAAGTC GAGGGGAGGA ATGGAATCTT TGGAGTTGAA AACTGGCAAG 
AGCAGTTGCT CCAGTGAGCA CACTGGTCAG GTTCTACTCT ACTGTATGAT GCAGTCGTCT 
CGATACGAGC AGCCTATTGG ACCCGGAAAT ATTCTATACC TTAAAGACGG CGCCTCCCAT 
TGTGTAACCC CGCGGGCTGC AGATCTTCTA GGCGTTCTAC AGCAACGGAA CAAGCTTTCA 
GTACACTTTG AGGATCCGAC TACATCAAAT CTTCCAGATC CACGTCAAGA CAGCAGATTT 
TGCGACAAAT GTGATCACAA GACGATGTGC TCATTCTATC AAAAAACCGA GGAGAACTTC 
TCGAAATCCA ACGATAAAAT GAAGAATTTC GCCGAGAATG AGATGGCTCA TCTGGAGCAG 
AATCACATAG AATACGCTGC AAATTGGATC CGCTGGATTT CAGCAGAATG GAAGTGCGAA 
CGAGAGCGAA TGACTTCTCA GAACAAGGAT CTCTGGCTCA AAAGTGTTCA AGAAAGAGTC 
GAAGAAGGAA CATGCCTGTC TGACCTGCAT CCTGTCTCCG AGGAAATGTC CAATTCGCAG 
AAGATTATCA TCAGTTTCTC GCGGAAATTT TTGAAATCTT CAAATACCCC TACATTCCGC 
CCGGGAGATG TCTGTCTGTT GTCGAATAAG AAGCATATTG CTATTGGATT CGCGTTGATT 
GATGAAGTAT CAGATGATCA ACAAATTGTC AAAATTTCCA CCGATAAAAC TATAAAATCT 
CGCTATGAAG CTCCGTTCCA TCTGGATAAA TACACAAGTA TGAGCACTCA TTCCACGACA 
CTCGGAAACC TGGTTTATTT ATTGCAAAAT GATGAAATTG GAAAACGACT AAGAAATATA 
CTCGTCGACC TGCTTCCTCC AATCATAATG GATGCTACTG GTTTTAATTT AACTCCAGCC 
ATCAAGAAAA TCATTATCAA GGCAAAGCTG AACAATGAAC AACGGAAAGC TGTAGTTCAC 
GCCCTGGCAA CTGAAGATTT TATGATGGTG GAGGGCCTGC CAGGATCTGG AAAAACGACT 
CTAATCTCTG TGCTCATTCA ATGTCTGGTA GCCACAAACA AGAAGGTCCT TCTGGCAGCA 
TTCACCCACT CGGCTGTTGA TAATATATTG ACAAAACTGA CAAAAGAGGT GGCGGCTGAA 
AAGATTCTCC GTCTTGGTAG CAGCTCTTCG ATCAAGGATG ACATCAAGAA AATGACTTTG 
AAAGCGAAGC TTGAGAATGA GACTTCTGAA GAGTACTATG CGGCAGTGAG AAAAGTCATG 
AAAACTACGC CAATCGTCGC TTGCACCTGC CACCACGTCC CTCGCGAGCT TCTATTCTCC 
TATCGCCACT TTGATGTTGT GATCGTTGAC GAAGCATCCA TGGTTCTAGA GCCCCTCCTG 
CTCCCAGTTC TCGCCACAAG CAACAAGTTC GTACTCGTTG GAGACTGCAA GCAGCTGACT 
CCGCTGGTAG TTTCAAGAAA GGCGAAGCAA GAAGGAGCTG GAATTTCGAC GATGGAGAAG 
CTTCAACAGT CTCATCCGGG CGTCGTCGTC TCGCTCACCT CACAATATCG AATGAATCGG 
GAGATTTCTG TGCTGTCCAG CAAACTTTTT TACGAGAATC GATTAATTTG TGGGAACGAA 
TCGGTTTCGC GGAGCTCTCT GGATAGGACT GGAGATTATA TTGTCGCGAT GGATGATGGT 
TCAGATCACA TTCGAAAAGC CCTTTCAGGA GATATCAAGG ATTCCTGCGT CTTCCTGGAC 
ACCCAGAGCA CAATTAATTC GAAAATGCAG TGCGAGGATG GAGAAGGCGG TGGAATGTGC 
AATGATGGAG AAGCCAAGCT CATTTCAGAG CTGTGCCAGC AGTTTGTAAT GAGTGGTGTA 
AAGCCGCATG AAATCGGTGT GATGTCCGCC TACCGTAGAC AAGTCGATCA TATTCGAGGA 
ATTTTAAATA GTGATGAGTT GGAGGTCAAC ACAATCGACT CGTACCAAGG ACGCGAGAAA 
CGTGTCATCA TCTGGTCACT AACCTGGACT AACAACTCGA CGAAGAAAAG CGAACTGCTC 
AAGGACGAGC GTCGTGTCAA TGTGGCGCTA ACCAGAGCCC GTCAGAAGCT AGTCGTTGTC 
GGATGCAAGA ACAGCGCTGA GACGATTCCA GTGCTTTTTA GATTCTCACA ATTAATTGAA 
AATCATATTT CTATTCCTAC TCAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496516.1
CDS	1..3210
Translation	MESRKRQLQNVNSESHEPKRLKEEFKGTEHDGDSVDSFENFCPTASSPKEKISPRSRSERIQLQECDDDDSFDAPVISSKPPALPKDSGVSGFPCLFDDKNVIHLTARKGEKRDDCLDLECIDKEGTTRIVYLQDHWSEMSIEAGSTINLIGAQAWGDRDFLVNDDVGIVILNPDALVPCTAVASATFCARKTVLNDKFKFGNASNKAMLLGTIMHEIFQTAITSKKRPVMENDLMKIWNKQAPKYAEELVALSFTPSCLDAELQPYFSIICGWINKHYPLSNSFFSKREPLPSKSELLEVYDIEENIWDSKLGLKGKIDVTMRTKSRGGMESLELKTGKSSCSSEHTGQVLLYCMMQSSRYEQPIGPGNILYLKDGASHCVTPRAADLLGVLQQRNKLSVHFEDPTTSNLPDPRQDSRFCDKCDHKTMCSFYQKTEENFSKSNDKMKNFAENEMAHLEQNHIEYAANWIRWISAEWKCERERMTSQNKDLWLKSVQERVEEGTCLSDLHPVSEEMSNSQKIIISFSRKFLKSSNTPTFRPGDVCLLSNKKHIAIGFALIDEVSDDQQIVKISTDKTIKSRYEAPFHLDKYTSMSTHSTTLGNLVYLLQNDEIGKRLRNILVDLLPPIIMDATGFNLTPAIKKIIIKAKLNNEQRKAVVHALATEDFMMVEGLPGSGKTTLISVLIQCLVATNKKVLLAAFTHSAVDNILTKLTKEVAAEKILRLGSSSSIKDDIKKMTLKAKLENETSEEYYAAVRKVMKTTPIVACTCHHVPRELLFSYRHFDVVIVDEASMVLEPLLLPVLATSNKFVLVGDCKQLTPLVVSRKAKQEGAGISTMEKLQQSHPGVVVSLTSQYRMNREISVLSSKLFYENRLICGNESVSRSSLDRTGDYIVAMDDGSDHIRKALSGDIKDSCVFLDTQSTINSKMQCEDGEGGGMCNDGEAKLISELCQQFVMSGVKPHEIGVMSAYRRQVDHIRGILNSDELEVNTIDSYQGREKRVIIWSLTWTNNSTKKSELLKDERRVNVALTRARQKLVVVGCKNSAETIPVLFRFSQLIENHISIPTQL

Target ORF information:

RefSeq Version	NM_064115.3
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans yeast DNA helicase/endonuclease family (dna-2), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064115.3

ORF Insert Sequence:

ATGGAGTCTC GCAAAAGACA GCTCCAAAAT GTCAATTCAG AATCACACGA GCCGAAACGT 
CTAAAAGAGG AATTCAAGGG AACCGAGCAT GATGGAGACA GCGTTGACTC GTTCGAGAAC 
TTCTGTCCGA CAGCTTCCTC TCCAAAAGAA AAAATCAGCC CGAGGTCGAG AAGTGAAAGG 
ATACAACTTC AAGAATGCGA CGATGACGAT TCGTTCGATG CACCAGTCAT TAGTTCAAAA 
CCTCCGGCTT TACCGAAAGA TAGCGGAGTT TCTGGATTCC CATGCCTCTT CGACGATAAA 
AATGTGATAC ATCTAACAGC TCGAAAAGGT GAAAAAAGAG ACGATTGTCT AGATTTGGAG 
TGTATAGACA AAGAAGGGAC GACTAGGATA GTCTATTTAC AGGATCATTG GTCGGAAATG 
AGCATTGAAG CAGGATCCAC GATTAACCTG ATCGGAGCTC AAGCATGGGG AGATCGTGAT 
TTCCTTGTCA ACGATGACGT CGGAATCGTG ATCTTAAATC CAGACGCACT TGTCCCGTGC 
ACAGCCGTCG CTTCAGCCAC ATTTTGTGCT CGAAAAACGG TGCTAAACGA CAAGTTTAAG 
TTTGGAAATG CCTCGAACAA AGCAATGCTT CTCGGAACCA TTATGCACGA GATATTCCAG 
ACAGCAATCA CGAGCAAAAA GCGACCTGTA ATGGAAAACG ATTTGATGAA AATTTGGAAC 
AAGCAGGCTC CAAAGTATGC CGAAGAGCTT GTGGCGCTCT CCTTTACTCC AAGCTGTCTG 
GACGCCGAGC TACAGCCTTA CTTTTCGATA ATCTGTGGCT GGATCAATAA ACATTATCCA 
CTTTCTAACA GCTTCTTCTC GAAACGCGAG CCTCTGCCGT CAAAATCGGA GCTTCTCGAG 
GTGTACGATA TTGAGGAGAA TATATGGGAT TCAAAGCTTG GTTTGAAGGG GAAGATCGAT 
GTGACGATGC GAACAAAGTC GAGGGGAGGA ATGGAATCTT TGGAGTTGAA AACTGGCAAG 
AGCAGTTGCT CCAGTGAGCA CACTGGTCAG GTTCTACTCT ACTGTATGAT GCAGTCGTCT 
CGATACGAGC AGCCTATTGG ACCCGGAAAT ATTCTATACC TTAAAGACGG CGCCTCCCAT 
TGTGTAACCC CGCGGGCTGC AGATCTTCTA GGCGTTCTAC AGCAACGGAA CAAGCTTTCA 
GTACACTTTG AGGATCCGAC TACATCAAAT CTTCCAGATC CACGTCAAGA CAGCAGATTT 
TGCGACAAAT GTGATCACAA GACGATGTGC TCATTCTATC AAAAAACCGA GGAGAACTTC 
TCGAAATCCA ACGATAAAAT GAAGAATTTC GCCGAGAATG AGATGGCTCA TCTGGAGCAG 
AATCACATAG AATACGCTGC AAATTGGATC CGCTGGATTT CAGCAGAATG GAAGTGCGAA 
CGAGAGCGAA TGACTTCTCA GAACAAGGAT CTCTGGCTCA AAAGTGTTCA AGAAAGAGTC 
GAAGAAGGAA CATGCCTGTC TGACCTGCAT CCTGTCTCCG AGGAAATGTC CAATTCGCAG 
AAGATTATCA TCAGTTTCTC GCGGAAATTT TTGAAATCTT CAAATACCCC TACATTCCGC 
CCGGGAGATG TCTGTCTGTT GTCGAATAAG AAGCATATTG CTATTGGATT CGCGTTGATT 
GATGAAGTAT CAGATGATCA ACAAATTGTC AAAATTTCCA CCGATAAAAC TATAAAATCT 
CGCTATGAAG CTCCGTTCCA TCTGGATAAA TACACAAGTA TGAGCACTCA TTCCACGACA 
CTCGGAAACC TGGTTTATTT ATTGCAAAAT GATGAAATTG GAAAACGACT AAGAAATATA 
CTCGTCGACC TGCTTCCTCC AATCATAATG GATGCTACTG GTTTTAATTT AACTCCAGCC 
ATCAAGAAAA TCATTATCAA GGCAAAGCTG AACAATGAAC AACGGAAAGC TGTAGTTCAC 
GCCCTGGCAA CTGAAGATTT TATGATGGTG GAGGGCCTGC CAGGATCTGG AAAAACGACT 
CTAATCTCTG TGCTCATTCA ATGTCTGGTA GCCACAAACA AGAAGGTCCT TCTGGCAGCA 
TTCACCCACT CGGCTGTTGA TAATATATTG ACAAAACTGA CAAAAGAGGT GGCGGCTGAA 
AAGATTCTCC GTCTTGGTAG CAGCTCTTCG ATCAAGGATG ACATCAAGAA AATGACTTTG 
AAAGCGAAGC TTGAGAATGA GACTTCTGAA GAGTACTATG CGGCAGTGAG AAAAGTCATG 
AAAACTACGC CAATCGTCGC TTGCACCTGC CACCACGTCC CTCGCGAGCT TCTATTCTCC 
TATCGCCACT TTGATGTTGT GATCGTTGAC GAAGCATCCA TGGTTCTAGA GCCCCTCCTG 
CTCCCAGTTC TCGCCACAAG CAACAAGTTC GTACTCGTTG GAGACTGCAA GCAGCTGACT 
CCGCTGGTAG TTTCAAGAAA GGCGAAGCAA GAAGGAGCTG GAATTTCGAC GATGGAGAAG 
CTTCAACAGT CTCATCCGGG CGTCGTCGTC TCGCTCACCT CACAATATCG AATGAATCGG 
GAGATTTCTG TGCTGTCCAG CAAACTTTTT TACGAGAATC GATTAATTTG TGGGAACGAA 
TCGGTTTCGC GGAGCTCTCT GGATAGGACT GGAGATTATA TTGTCGCGAT GGATGATGGT 
TCAGATCACA TTCGAAAAGC CCTTTCAGGA GATATCAAGG ATTCCTGCGT CTTCCTGGAC 
ACCCAGAGCA CAATTAATTC GAAAATGCAG TGCGAGGATG GAGAAGGCGG TGGAATGTGC 
AATGATGGAG AAGCCAAGCT CATTTCAGAG CTGTGCCAGC AGTTTGTAAT GAGTGGTGTA 
AAGCCGCATG AAATCGGTGT GATGTCCGCC TACCGTAGAC AAGTCGATCA TATTCGAGGA 
ATTTTAAATA GTGATGAGTT GGAGGTCAAC ACAATCGACT CGTACCAAGG ACGCGAGAAA 
CGTGTCATCA TCTGGTCACT AACCTGGACT AACAACTCGA CGAAGAAAAG CGAACTGCTC 
AAGGACGAGC GTCGTGTCAA TGTGGCGCTA ACCAGAGCCC GTCAGAAGCT AGTCGTTGTC 
GGATGCAAGA ACAGCGCTGA GACGATTCCA GTGCTTTTTA GATTCTCACA ATTAATTGAA 
AATCATATTT CTATTCCTAC TCAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

dna-2 ( NM_064115.4 ) cDNA ORF clone, Caenorhabditis elegans -> NP_496516.1 Caenorhabditis elegans yeast DNA helicase/endonuclease family (dna-2), mRNA.

Price & Availability↑

CloneID	OCa338234
Clone ID Related Accession (Same CDS sequence)	NM_064115.3 , NM_064115.4
Accession Version	NM_064115.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-17
Organism	Caenorhabditis elegans
Product	yeast DNA helicase/endonuclease family
Comment	Comment: REVIEWED REFSEQ: This record has been curated by WormBase. This record is derived from an annotated genomic sequence (NC_003280). On Sep 4, 2019 this sequence version replaced NM_064115.3.

ATGGAGTCTC GCAAAAGACA GCTCCAAAAT GTCAATTCAG AATCACACGA GCCGAAACGT 
CTAAAAGAGG AATTCAAGGG AACCGAGCAT GATGGAGACA GCGTTGACTC GTTCGAGAAC 
TTCTGTCCGA CAGCTTCCTC TCCAAAAGAA AAAATCAGCC CGAGGTCGAG AAGTGAAAGG 
ATACAACTTC AAGAATGCGA CGATGACGAT TCGTTCGATG CACCAGTCAT TAGTTCAAAA 
CCTCCGGCTT TACCGAAAGA TAGCGGAGTT TCTGGATTCC CATGCCTCTT CGACGATAAA 
AATGTGATAC ATCTAACAGC TCGAAAAGGT GAAAAAAGAG ACGATTGTCT AGATTTGGAG 
TGTATAGACA AAGAAGGGAC GACTAGGATA GTCTATTTAC AGGATCATTG GTCGGAAATG 
AGCATTGAAG CAGGATCCAC GATTAACCTG ATCGGAGCTC AAGCATGGGG AGATCGTGAT 
TTCCTTGTCA ACGATGACGT CGGAATCGTG ATCTTAAATC CAGACGCACT TGTCCCGTGC 
ACAGCCGTCG CTTCAGCCAC ATTTTGTGCT CGAAAAACGG TGCTAAACGA CAAGTTTAAG 
TTTGGAAATG CCTCGAACAA AGCAATGCTT CTCGGAACCA TTATGCACGA GATATTCCAG 
ACAGCAATCA CGAGCAAAAA GCGACCTGTA ATGGAAAACG ATTTGATGAA AATTTGGAAC 
AAGCAGGCTC CAAAGTATGC CGAAGAGCTT GTGGCGCTCT CCTTTACTCC AAGCTGTCTG 
GACGCCGAGC TACAGCCTTA CTTTTCGATA ATCTGTGGCT GGATCAATAA ACATTATCCA 
CTTTCTAACA GCTTCTTCTC GAAACGCGAG CCTCTGCCGT CAAAATCGGA GCTTCTCGAG 
GTGTACGATA TTGAGGAGAA TATATGGGAT TCAAAGCTTG GTTTGAAGGG GAAGATCGAT 
GTGACGATGC GAACAAAGTC GAGGGGAGGA ATGGAATCTT TGGAGTTGAA AACTGGCAAG 
AGCAGTTGCT CCAGTGAGCA CACTGGTCAG GTTCTACTCT ACTGTATGAT GCAGTCGTCT 
CGATACGAGC AGCCTATTGG ACCCGGAAAT ATTCTATACC TTAAAGACGG CGCCTCCCAT 
TGTGTAACCC CGCGGGCTGC AGATCTTCTA GGCGTTCTAC AGCAACGGAA CAAGCTTTCA 
GTACACTTTG AGGATCCGAC TACATCAAAT CTTCCAGATC CACGTCAAGA CAGCAGATTT 
TGCGACAAAT GTGATCACAA GACGATGTGC TCATTCTATC AAAAAACCGA GGAGAACTTC 
TCGAAATCCA ACGATAAAAT GAAGAATTTC GCCGAGAATG AGATGGCTCA TCTGGAGCAG 
AATCACATAG AATACGCTGC AAATTGGATC CGCTGGATTT CAGCAGAATG GAAGTGCGAA 
CGAGAGCGAA TGACTTCTCA GAACAAGGAT CTCTGGCTCA AAAGTGTTCA AGAAAGAGTC 
GAAGAAGGAA CATGCCTGTC TGACCTGCAT CCTGTCTCCG AGGAAATGTC CAATTCGCAG 
AAGATTATCA TCAGTTTCTC GCGGAAATTT TTGAAATCTT CAAATACCCC TACATTCCGC 
CCGGGAGATG TCTGTCTGTT GTCGAATAAG AAGCATATTG CTATTGGATT CGCGTTGATT 
GATGAAGTAT CAGATGATCA ACAAATTGTC AAAATTTCCA CCGATAAAAC TATAAAATCT 
CGCTATGAAG CTCCGTTCCA TCTGGATAAA TACACAAGTA TGAGCACTCA TTCCACGACA 
CTCGGAAACC TGGTTTATTT ATTGCAAAAT GATGAAATTG GAAAACGACT AAGAAATATA 
CTCGTCGACC TGCTTCCTCC AATCATAATG GATGCTACTG GTTTTAATTT AACTCCAGCC 
ATCAAGAAAA TCATTATCAA GGCAAAGCTG AACAATGAAC AACGGAAAGC TGTAGTTCAC 
GCCCTGGCAA CTGAAGATTT TATGATGGTG GAGGGCCTGC CAGGATCTGG AAAAACGACT 
CTAATCTCTG TGCTCATTCA ATGTCTGGTA GCCACAAACA AGAAGGTCCT TCTGGCAGCA 
TTCACCCACT CGGCTGTTGA TAATATATTG ACAAAACTGA CAAAAGAGGT GGCGGCTGAA 
AAGATTCTCC GTCTTGGTAG CAGCTCTTCG ATCAAGGATG ACATCAAGAA AATGACTTTG 
AAAGCGAAGC TTGAGAATGA GACTTCTGAA GAGTACTATG CGGCAGTGAG AAAAGTCATG 
AAAACTACGC CAATCGTCGC TTGCACCTGC CACCACGTCC CTCGCGAGCT TCTATTCTCC 
TATCGCCACT TTGATGTTGT GATCGTTGAC GAAGCATCCA TGGTTCTAGA GCCCCTCCTG 
CTCCCAGTTC TCGCCACAAG CAACAAGTTC GTACTCGTTG GAGACTGCAA GCAGCTGACT 
CCGCTGGTAG TTTCAAGAAA GGCGAAGCAA GAAGGAGCTG GAATTTCGAC GATGGAGAAG 
CTTCAACAGT CTCATCCGGG CGTCGTCGTC TCGCTCACCT CACAATATCG AATGAATCGG 
GAGATTTCTG TGCTGTCCAG CAAACTTTTT TACGAGAATC GATTAATTTG TGGGAACGAA 
TCGGTTTCGC GGAGCTCTCT GGATAGGACT GGAGATTATA TTGTCGCGAT GGATGATGGT 
TCAGATCACA TTCGAAAAGC CCTTTCAGGA GATATCAAGG ATTCCTGCGT CTTCCTGGAC 
ACCCAGAGCA CAATTAATTC GAAAATGCAG TGCGAGGATG GAGAAGGCGG TGGAATGTGC 
AATGATGGAG AAGCCAAGCT CATTTCAGAG CTGTGCCAGC AGTTTGTAAT GAGTGGTGTA 
AAGCCGCATG AAATCGGTGT GATGTCCGCC TACCGTAGAC AAGTCGATCA TATTCGAGGA 
ATTTTAAATA GTGATGAGTT GGAGGTCAAC ACAATCGACT CGTACCAAGG ACGCGAGAAA 
CGTGTCATCA TCTGGTCACT AACCTGGACT AACAACTCGA CGAAGAAAAG CGAACTGCTC 
AAGGACGAGC GTCGTGTCAA TGTGGCGCTA ACCAGAGCCC GTCAGAAGCT AGTCGTTGTC 
GGATGCAAGA ACAGCGCTGA GACGATTCCA GTGCTTTTTA GATTCTCACA ATTAATTGAA 
AATCATATTT CTATTCCTAC TCAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_496516.1
CDS	19..3228
Translation	MESRKRQLQNVNSESHEPKRLKEEFKGTEHDGDSVDSFENFCPTASSPKEKISPRSRSERIQLQECDDDDSFDAPVISSKPPALPKDSGVSGFPCLFDDKNVIHLTARKGEKRDDCLDLECIDKEGTTRIVYLQDHWSEMSIEAGSTINLIGAQAWGDRDFLVNDDVGIVILNPDALVPCTAVASATFCARKTVLNDKFKFGNASNKAMLLGTIMHEIFQTAITSKKRPVMENDLMKIWNKQAPKYAEELVALSFTPSCLDAELQPYFSIICGWINKHYPLSNSFFSKREPLPSKSELLEVYDIEENIWDSKLGLKGKIDVTMRTKSRGGMESLELKTGKSSCSSEHTGQVLLYCMMQSSRYEQPIGPGNILYLKDGASHCVTPRAADLLGVLQQRNKLSVHFEDPTTSNLPDPRQDSRFCDKCDHKTMCSFYQKTEENFSKSNDKMKNFAENEMAHLEQNHIEYAANWIRWISAEWKCERERMTSQNKDLWLKSVQERVEEGTCLSDLHPVSEEMSNSQKIIISFSRKFLKSSNTPTFRPGDVCLLSNKKHIAIGFALIDEVSDDQQIVKISTDKTIKSRYEAPFHLDKYTSMSTHSTTLGNLVYLLQNDEIGKRLRNILVDLLPPIIMDATGFNLTPAIKKIIIKAKLNNEQRKAVVHALATEDFMMVEGLPGSGKTTLISVLIQCLVATNKKVLLAAFTHSAVDNILTKLTKEVAAEKILRLGSSSSIKDDIKKMTLKAKLENETSEEYYAAVRKVMKTTPIVACTCHHVPRELLFSYRHFDVVIVDEASMVLEPLLLPVLATSNKFVLVGDCKQLTPLVVSRKAKQEGAGISTMEKLQQSHPGVVVSLTSQYRMNREISVLSSKLFYENRLICGNESVSRSSLDRTGDYIVAMDDGSDHIRKALSGDIKDSCVFLDTQSTINSKMQCEDGEGGGMCNDGEAKLISELCQQFVMSGVKPHEIGVMSAYRRQVDHIRGILNSDELEVNTIDSYQGREKRVIIWSLTWTNNSTKKSELLKDERRVNVALTRARQKLVVVGCKNSAETIPVLFRFSQLIENHISIPTQL

Target ORF information:

RefSeq Version	NM_064115.4
Organism	Caenorhabditis elegans
Definition	Caenorhabditis elegans yeast DNA helicase/endonuclease family (dna-2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_064115.4

ORF Insert Sequence:

ATGGAGTCTC GCAAAAGACA GCTCCAAAAT GTCAATTCAG AATCACACGA GCCGAAACGT 
CTAAAAGAGG AATTCAAGGG AACCGAGCAT GATGGAGACA GCGTTGACTC GTTCGAGAAC 
TTCTGTCCGA CAGCTTCCTC TCCAAAAGAA AAAATCAGCC CGAGGTCGAG AAGTGAAAGG 
ATACAACTTC AAGAATGCGA CGATGACGAT TCGTTCGATG CACCAGTCAT TAGTTCAAAA 
CCTCCGGCTT TACCGAAAGA TAGCGGAGTT TCTGGATTCC CATGCCTCTT CGACGATAAA 
AATGTGATAC ATCTAACAGC TCGAAAAGGT GAAAAAAGAG ACGATTGTCT AGATTTGGAG 
TGTATAGACA AAGAAGGGAC GACTAGGATA GTCTATTTAC AGGATCATTG GTCGGAAATG 
AGCATTGAAG CAGGATCCAC GATTAACCTG ATCGGAGCTC AAGCATGGGG AGATCGTGAT 
TTCCTTGTCA ACGATGACGT CGGAATCGTG ATCTTAAATC CAGACGCACT TGTCCCGTGC 
ACAGCCGTCG CTTCAGCCAC ATTTTGTGCT CGAAAAACGG TGCTAAACGA CAAGTTTAAG 
TTTGGAAATG CCTCGAACAA AGCAATGCTT CTCGGAACCA TTATGCACGA GATATTCCAG 
ACAGCAATCA CGAGCAAAAA GCGACCTGTA ATGGAAAACG ATTTGATGAA AATTTGGAAC 
AAGCAGGCTC CAAAGTATGC CGAAGAGCTT GTGGCGCTCT CCTTTACTCC AAGCTGTCTG 
GACGCCGAGC TACAGCCTTA CTTTTCGATA ATCTGTGGCT GGATCAATAA ACATTATCCA 
CTTTCTAACA GCTTCTTCTC GAAACGCGAG CCTCTGCCGT CAAAATCGGA GCTTCTCGAG 
GTGTACGATA TTGAGGAGAA TATATGGGAT TCAAAGCTTG GTTTGAAGGG GAAGATCGAT 
GTGACGATGC GAACAAAGTC GAGGGGAGGA ATGGAATCTT TGGAGTTGAA AACTGGCAAG 
AGCAGTTGCT CCAGTGAGCA CACTGGTCAG GTTCTACTCT ACTGTATGAT GCAGTCGTCT 
CGATACGAGC AGCCTATTGG ACCCGGAAAT ATTCTATACC TTAAAGACGG CGCCTCCCAT 
TGTGTAACCC CGCGGGCTGC AGATCTTCTA GGCGTTCTAC AGCAACGGAA CAAGCTTTCA 
GTACACTTTG AGGATCCGAC TACATCAAAT CTTCCAGATC CACGTCAAGA CAGCAGATTT 
TGCGACAAAT GTGATCACAA GACGATGTGC TCATTCTATC AAAAAACCGA GGAGAACTTC 
TCGAAATCCA ACGATAAAAT GAAGAATTTC GCCGAGAATG AGATGGCTCA TCTGGAGCAG 
AATCACATAG AATACGCTGC AAATTGGATC CGCTGGATTT CAGCAGAATG GAAGTGCGAA 
CGAGAGCGAA TGACTTCTCA GAACAAGGAT CTCTGGCTCA AAAGTGTTCA AGAAAGAGTC 
GAAGAAGGAA CATGCCTGTC TGACCTGCAT CCTGTCTCCG AGGAAATGTC CAATTCGCAG 
AAGATTATCA TCAGTTTCTC GCGGAAATTT TTGAAATCTT CAAATACCCC TACATTCCGC 
CCGGGAGATG TCTGTCTGTT GTCGAATAAG AAGCATATTG CTATTGGATT CGCGTTGATT 
GATGAAGTAT CAGATGATCA ACAAATTGTC AAAATTTCCA CCGATAAAAC TATAAAATCT 
CGCTATGAAG CTCCGTTCCA TCTGGATAAA TACACAAGTA TGAGCACTCA TTCCACGACA 
CTCGGAAACC TGGTTTATTT ATTGCAAAAT GATGAAATTG GAAAACGACT AAGAAATATA 
CTCGTCGACC TGCTTCCTCC AATCATAATG GATGCTACTG GTTTTAATTT AACTCCAGCC 
ATCAAGAAAA TCATTATCAA GGCAAAGCTG AACAATGAAC AACGGAAAGC TGTAGTTCAC 
GCCCTGGCAA CTGAAGATTT TATGATGGTG GAGGGCCTGC CAGGATCTGG AAAAACGACT 
CTAATCTCTG TGCTCATTCA ATGTCTGGTA GCCACAAACA AGAAGGTCCT TCTGGCAGCA 
TTCACCCACT CGGCTGTTGA TAATATATTG ACAAAACTGA CAAAAGAGGT GGCGGCTGAA 
AAGATTCTCC GTCTTGGTAG CAGCTCTTCG ATCAAGGATG ACATCAAGAA AATGACTTTG 
AAAGCGAAGC TTGAGAATGA GACTTCTGAA GAGTACTATG CGGCAGTGAG AAAAGTCATG 
AAAACTACGC CAATCGTCGC TTGCACCTGC CACCACGTCC CTCGCGAGCT TCTATTCTCC 
TATCGCCACT TTGATGTTGT GATCGTTGAC GAAGCATCCA TGGTTCTAGA GCCCCTCCTG 
CTCCCAGTTC TCGCCACAAG CAACAAGTTC GTACTCGTTG GAGACTGCAA GCAGCTGACT 
CCGCTGGTAG TTTCAAGAAA GGCGAAGCAA GAAGGAGCTG GAATTTCGAC GATGGAGAAG 
CTTCAACAGT CTCATCCGGG CGTCGTCGTC TCGCTCACCT CACAATATCG AATGAATCGG 
GAGATTTCTG TGCTGTCCAG CAAACTTTTT TACGAGAATC GATTAATTTG TGGGAACGAA 
TCGGTTTCGC GGAGCTCTCT GGATAGGACT GGAGATTATA TTGTCGCGAT GGATGATGGT 
TCAGATCACA TTCGAAAAGC CCTTTCAGGA GATATCAAGG ATTCCTGCGT CTTCCTGGAC 
ACCCAGAGCA CAATTAATTC GAAAATGCAG TGCGAGGATG GAGAAGGCGG TGGAATGTGC 
AATGATGGAG AAGCCAAGCT CATTTCAGAG CTGTGCCAGC AGTTTGTAAT GAGTGGTGTA 
AAGCCGCATG AAATCGGTGT GATGTCCGCC TACCGTAGAC AAGTCGATCA TATTCGAGGA 
ATTTTAAATA GTGATGAGTT GGAGGTCAAC ACAATCGACT CGTACCAAGG ACGCGAGAAA 
CGTGTCATCA TCTGGTCACT AACCTGGACT AACAACTCGA CGAAGAAAAG CGAACTGCTC 
AAGGACGAGC GTCGTGTCAA TGTGGCGCTA ACCAGAGCCC GTCAGAAGCT AGTCGTTGTC 
GGATGCAAGA ACAGCGCTGA GACGATTCCA GTGCTTTTTA GATTCTCACA ATTAATTGAA 
AATCATATTT CTATTCCTAC TCAACTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome sequence of the nematode C. elegans: a platform for investigating biology.
Science (New York, N.Y.)282(5396)2012-8(1998 Dec)

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dna-2 cDNA ORF clone, Caenorhabditis elegans

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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