ORF » Species Summary » Saccharomyces cerevisiae S288C » ASI1 cDNA ORF clone

ASI1 cDNA ORF clone, Saccharomyces cerevisiae S288C

The following ASI1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ASI1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSi04115 NM_001182619.1
Latest version!		 Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase ASI1 (ASI1), partial mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    ASI1 ( NM_001182619.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_013837.1 Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase ASI1 (ASI1), partial mRNA.
    Price & Availability↑
    CloneID OSi04115
    Clone ID Related Accession (Same CDS sequence) NM_001182619.1
    Accession Version NM_001182619.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1875bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-10-31
    Organism Saccharomyces cerevisiae S288C
    Product putative ubiquitin-protein ligase ASI1
    Comment Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001145). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAACTCTT CCACTTCTTC AGAAAATGTG TTCATAAATT CATTTAGCTA TTTGAATCAA 
    ACATCACAAG CCGTAATCTC AGGTAACTCC ACTTTCGCAA ATGTTATCAA CTTTCCTTAT 
    AGACTTGGAT TATCCTTTAT TGGGGCTGTG AACTTGCAGT ACGAGCAAAC CGTTAAATCT 
    GAAGAAATTC CCCCAACTCT AAGGTCTGTT TTTGATACAA TAGGGTTTTT TTTCAGCCCG 
    TACGCCATAT TTTGCTTTGT AATCGCCATT GTCCTGAACC GGTTCGTTGT ATTTTACGCC 
    GTCCTTAACA ACGGTTCCAG ACGTACTTTA CCGTTATGGT TGAGTAATGT TTTCCATGTA 
    TCAGCAGTAG TTGTATTAGC AATGGTATCC TTAGGTCCGC TGACCTTGGG TAAGGATTTC 
    AAAATATTAG GCGACCCTGC TTTTGCCCAA GAAAAATTTT TACTGAATAT ATTCTACGCT 
    TTCGCTTACT CTTATTGTGT CGAAACCATA TTTACCATCA TGCGGAATTC CAGCCCTTTG 
    GAGGGCACTG ACTATTCATT GTTTGAACTT TCGATTCAGT TTTATACAAT GACTAATAAT 
    AATACTAAAT TTTTAGATTC TCCTGATTAC ATTATAGACT GTTCTATGGC TATTTTGAGC 
    AGGATATTGA TCCATTTGGT GGAGATTTTC AGGCTTAGAA ATTATCGTCT ACTTTTTAGT 
    ACTATTATGA ACCTCTGTCA TATTTGTTAC TTAGGGATAA GAGTTAAGCA GGGCGGCTGG 
    AAAAGCCTTC CATTTTCGGT GAAATTTAGG CACTTTCCAA AACTGTTTTC AGTGTCTATT 
    ATTTGCTTAT CATTACTGAT TTTTAAATTA TCATGCTTAA TTAGATGGGA TCCATTTGGA 
    AAATCACGTA ATTCTTGCGA ACTTTTACAG TTTTACCCTC TCAGTAGAAA TTGGAAAAAA 
    TACTTAAATT ACACGGGAGA AGAAGATTTT TCTGCTATGG CCACTAAATT CGCACTATTA 
    TTATGCTCAG GAACTGAACT AATGGAAAAG GGTATACGAA GAGAATTTCC TGCTATTAAC 
    ATCCCGGATA ATGTTAATGA GAAATTTTTT ATTAGCGGTT ATTTAAATGA ACTTTCTAAA 
    CCGTATAAAG AGAACACTTC CATCAGCTTC CCAAAGAAAA ATTCATCAAT ATTAAAGCAA 
    AGATTTTTTT TAATGTTTCC AAAATCTATA ATATGGATCA TGAAGAAGCT TGTAGGCCAA 
    GTATTCTTTG GCTTCAGAGA TAACAAGGAC GAGGACATCC CCGACAACGA CCCATCAAAA 
    ATGTTAAAGA TAACTAAAAC GAACTCTCTC AATAATAGTG CCGGCCACAA AGAAGATATT 
    GAACTTGAAC TATTGAACAC ATCAGATGAT GAGTATTCTG AAGATTACGA GCCTTCTGAA 
    GTTGAGTCAC TGGGGGATTC TGATGAAGAA AACTTGGAAG AAGATTCACT GATTTTCAAC 
    GAAACTCGGG ATGCCCTACT GGATTTATTT TCTTCCGAAG ATAATGAAGT TCATACTGAT 
    TATAATTGGA TCATGTCAAC TAGCCGTATC CTTCAACAAA AATTACTGTC AGATAAAACT 
    TTGACAAGGG CGTCTATCTT AGATACAAAG TTAAGTGAAG TAGATGAGAC TTTTGGTACA 
    GAAAGTGATT TTGATCTATC TTGCGCGGTA TGCAAAGTCA ATGAAAGAAA TACAGTATTA 
    TGGCCCTGCC GATGTTTTGC CATTTGCGAG GATTGCAGAA TTTCTTTGGG TTTACGTGGG 
    TTCAGCACTT GCGTATGTTG TAGGAGTAAA GTTCATGGGT ACTGTAAGGT TCATCCTGTT 
    TCAGATAGTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_013837.1
    CDS1..1875
    Translation

    Target ORF information:

    RefSeq Version NM_001182619.1
    Organism Saccharomyces cerevisiae S288C
    Definition Saccharomyces cerevisiae S288C putative ubiquitin-protein ligase ASI1 (ASI1), partial mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001182619.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGAACTCTT CCACTTCTTC AGAAAATGTG TTCATAAATT CATTTAGCTA TTTGAATCAA 
    ACATCACAAG CCGTAATCTC AGGTAACTCC ACTTTCGCAA ATGTTATCAA CTTTCCTTAT 
    AGACTTGGAT TATCCTTTAT TGGGGCTGTG AACTTGCAGT ACGAGCAAAC CGTTAAATCT 
    GAAGAAATTC CCCCAACTCT AAGGTCTGTT TTTGATACAA TAGGGTTTTT TTTCAGCCCG 
    TACGCCATAT TTTGCTTTGT AATCGCCATT GTCCTGAACC GGTTCGTTGT ATTTTACGCC 
    GTCCTTAACA ACGGTTCCAG ACGTACTTTA CCGTTATGGT TGAGTAATGT TTTCCATGTA 
    TCAGCAGTAG TTGTATTAGC AATGGTATCC TTAGGTCCGC TGACCTTGGG TAAGGATTTC 
    AAAATATTAG GCGACCCTGC TTTTGCCCAA GAAAAATTTT TACTGAATAT ATTCTACGCT 
    TTCGCTTACT CTTATTGTGT CGAAACCATA TTTACCATCA TGCGGAATTC CAGCCCTTTG 
    GAGGGCACTG ACTATTCATT GTTTGAACTT TCGATTCAGT TTTATACAAT GACTAATAAT 
    AATACTAAAT TTTTAGATTC TCCTGATTAC ATTATAGACT GTTCTATGGC TATTTTGAGC 
    AGGATATTGA TCCATTTGGT GGAGATTTTC AGGCTTAGAA ATTATCGTCT ACTTTTTAGT 
    ACTATTATGA ACCTCTGTCA TATTTGTTAC TTAGGGATAA GAGTTAAGCA GGGCGGCTGG 
    AAAAGCCTTC CATTTTCGGT GAAATTTAGG CACTTTCCAA AACTGTTTTC AGTGTCTATT 
    ATTTGCTTAT CATTACTGAT TTTTAAATTA TCATGCTTAA TTAGATGGGA TCCATTTGGA 
    AAATCACGTA ATTCTTGCGA ACTTTTACAG TTTTACCCTC TCAGTAGAAA TTGGAAAAAA 
    TACTTAAATT ACACGGGAGA AGAAGATTTT TCTGCTATGG CCACTAAATT CGCACTATTA 
    TTATGCTCAG GAACTGAACT AATGGAAAAG GGTATACGAA GAGAATTTCC TGCTATTAAC 
    ATCCCGGATA ATGTTAATGA GAAATTTTTT ATTAGCGGTT ATTTAAATGA ACTTTCTAAA 
    CCGTATAAAG AGAACACTTC CATCAGCTTC CCAAAGAAAA ATTCATCAAT ATTAAAGCAA 
    AGATTTTTTT TAATGTTTCC AAAATCTATA ATATGGATCA TGAAGAAGCT TGTAGGCCAA 
    GTATTCTTTG GCTTCAGAGA TAACAAGGAC GAGGACATCC CCGACAACGA CCCATCAAAA 
    ATGTTAAAGA TAACTAAAAC GAACTCTCTC AATAATAGTG CCGGCCACAA AGAAGATATT 
    GAACTTGAAC TATTGAACAC ATCAGATGAT GAGTATTCTG AAGATTACGA GCCTTCTGAA 
    GTTGAGTCAC TGGGGGATTC TGATGAAGAA AACTTGGAAG AAGATTCACT GATTTTCAAC 
    GAAACTCGGG ATGCCCTACT GGATTTATTT TCTTCCGAAG ATAATGAAGT TCATACTGAT 
    TATAATTGGA TCATGTCAAC TAGCCGTATC CTTCAACAAA AATTACTGTC AGATAAAACT 
    TTGACAAGGG CGTCTATCTT AGATACAAAG TTAAGTGAAG TAGATGAGAC TTTTGGTACA 
    GAAAGTGATT TTGATCTATC TTGCGCGGTA TGCAAAGTCA ATGAAAGAAA TACAGTATTA 
    TGGCCCTGCC GATGTTTTGC CATTTGCGAG GATTGCAGAA TTTCTTTGGG TTTACGTGGG 
    TTCAGCACTT GCGTATGTTG TAGGAGTAAA GTTCATGGGT ACTGTAAGGT TCATCCTGTT 
    TCAGATAGTA AATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.
    Nature387(6632 Suppl)90-3(1997 May)
    Bowman S,Churcher C,Badcock K,Brown D,Chillingworth T,Connor R,Dedman K,Devlin K,Gentles S,Hamlin N,Hunt S,Jagels K,Lye G,Moule S,Odell C,Pearson D,Rajandream M,Rice P,Skelton J,Walsh S,Whitehead S,Barrell B


    Life with 6000 genes.
    Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
    Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG


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