VPH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	VPH1
Entrez Gene ID	854444
Full Name	H(+)-transporting V0 sector ATPase subunit a
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001183689.3	NP_014913.3	H(+)-transporting V0 sector ATPase subunit a

Pathways from BioSystems

Homologies

Homo sapiens (human)	ATP6V0A1	NP_001123493.1
Bos taurus (cattle)	ATP6V0A1	NP_777179.1
Mus musculus (house mouse)	Atp6v0a1	NP_058616.1
Saccharomyces cerevisiae (baker's yeast)	VPH1	NP_014913.3
Kluyveromyces lactis	KLLA0C07491g	XP_452533.1
Eremothecium gossypii	AGOS_ADR177C	NP_984273.2
Schizosaccharomyces pombe (fission yeast)	vph1	NP_594219.2
Gallus gallus (chicken)	ATP6V0A1	NP_990055.1
Drosophila melanogaster (fruit fly)	Vha100-1	NP_651672.1
Caenorhabditis elegans (roundworm)	unc-32	NP_001023017.1
Magnaporthe oryzae (rice blast fungus)	MGG_03947	XP_003719917.1
Arabidopsis thaliana (thale cress)	VHA-A1	NP_850122.1
Oryza sativa (rice)	Os01g0834200	NP_001044718.2
Rattus norvegicus (Norway rat)	Atp6v0a1	NP_113792.2
Neurospora crassa	NCU03463	XP_956054.1
Pan troglodytes (chimpanzee)	ATP6V0A1	XP_001165034.1
	ATP6V0A1	XP_001095161.2
Canis lupus familiaris (dog)	ATP6V0A1	XP_849622.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003711	XP_003436582.1
Xenopus tropicalis (tropical clawed frog)	atp6v0a1	NP_001090714.1

Gene Ontology
Bibliography

Related articles in PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Dujon B, Albermann K, Aldea M, Alexandraki D, Ansorge W, Arino J, Benes V, Bohn C, Bolotin-Fukuhara M, Bordonn? R, Boyer J, Camasses A, Casamayor A, Casas C, Ch?ret G, Cziepluch C, Daignan-Fornier B, Dang DV, de Haan M, Delius H, Durand P, Fairhead C, Feldmann H, Gaillon L, Kleine K
Nature387(6632 Suppl)98-102(1997 May)

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

we show that the role of Sch9 in regulating ageing is tightly connected with V-ATPase activity and vacuolar acidity. As both Sch9 and the V-ATPase are highly conserved in higher eukaryotes, it will be interesting to further clarify their cooperative action on the cellular processes that influence growth and ageing.
Title: The yeast protein kinase Sch9 adjusts V-ATPase assembly/disassembly to control pH homeostasis and longevity in response to glucose availability.

We speculate that the spatial closeness of the a(NT), C(foot), and EG binding sites in the intact V-ATPase results in a high-avidity interaction that is able to resist the torque of rotational catalysis.
Title: Subunit interactions at the V1-Vo interface in yeast vacuolar ATPase.

Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p).
Title: Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p).

Consequences of loss of Vph1 protein-containing vacuolar ATPases (V-ATPases) for overall cellular pH homeostasis.
Title: Consequences of loss of Vph1 protein-containing vacuolar ATPases (V-ATPases) for overall cellular pH homeostasis.

Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor.
Title: Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor.

The following VPH1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the VPH1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi03561	NM_001183689.3 Latest version!	Saccharomyces cerevisiae S288C H(+)-transporting V0 sector ATPase subunit a (VPH1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

VPH1 ( NM_001183689.3 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_014913.3 Saccharomyces cerevisiae S288C H(+)-transporting V0 sector ATPase subunit a (VPH1), partial mRNA.

Price & Availability↑

CloneID	OSi03561
Clone ID Related Accession (Same CDS sequence)	NM_001183689.3
Accession Version	NM_001183689.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2523bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	H(+)-transporting V0 sector ATPase subunit a
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001147). On Jul 30, 2012 this sequence version replaced NM_001183689.2. ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGCAGAGA AGGAGGAAGC GATTTTTCGC TCTGCTGAAA TGGCTTTAGT CCAATTCTAT 
ATTCCTCAAG AAATTTCAAG AGACTCTGCT TACACTTTAG GTCAATTGGG TCTTGTTCAA 
TTCCGTGACT TGAACTCTAA GGTGCGTGCG TTTCAAAGAA CTTTCGTGAA CGAAATTAGA 
AGACTGGATA ATGTAGAAAG ACAGTATCGT TACTTTTATT CTCTTTTGAA GAAACACGAT 
ATTAAGCTCT ACGAAGGAGA CACGGACAAA TATTTGGACG GCTCAGGTGA ATTGTACGTT 
CCACCAAGCG GTTCAGTGAT AGATGATTAT GTCCGGAACG CTTCATATTT GGAAGAAAGA 
TTGATTCAAA TGGAGGATGC AACCGATCAA ATTGAAGTCC AGAAAAATGA CTTGGAACAG 
TATCGCTTTA TTTTGCAGTC AGGTGATGAA TTTTTCTTGA AGGGTGATAA TACCGACAGC 
ACTTCCTATA TGGATGAAGA CATGATCGAC GCTAATGGGG AAAACATTGC TGCTGCTATC 
GGTGCTTCTG TAAACTATGT CACTGGTGTC ATTGCTAGAG ACAAAGTTGC CACCTTAGAA 
CAAATTCTTT GGAGAGTATT AAGAGGTAAC CTTTTCTTCA AAACTGTTGA AATTGAACAA 
CCTGTTTATG ATGTCAAAAC CAGGGAGTAT AAACATAAAA ATGCTTTTAT CGTATTTTCT 
CACGGTGATC TGATTATTAA AAGAATCAGA AAGATTGCGG AATCATTGGA TGCCAATCTT 
TACGATGTTG ACTCTTCCAA CGAGGGTAGA TCACAACAAT TGGCCAAGGT CAACAAGAAT 
TTGAGTGATT TGTACACAGT TTTGAAAACC ACTTCTACCA CTTTAGAAAG TGAATTATAT 
GCCATTGCCA AAGAATTGGA CTCTTGGTTC CAAGATGTTA CCCGTGAAAA GGCGATTTTT 
GAAATTTTGA ACAAGTCTAA CTATGATACC AATAGAAAGA TTTTGATTGC TGAAGGTTGG 
ATACCAAGAG ACGAATTGGC TACTTTGCAA GCTCGTCTTG GTGAAATGAT CGCAAGATTG 
GGTATTGATG TCCCATCCAT TATCCAAGTC CTGGATACAA ACCACACTCC ACCTACCTTC 
CACAGAACTA ACAAGTTTAC TGCTGGTTTC CAAAGTATCT GTGACTGTTA CGGTATTGCT 
CAGTACAGAG AAATCAATGC TGGTTTACCC ACAATTGTCA CTTTCCCTTT CATGTTTGCC 
ATCATGTTTG GTGATATGGG TCACGGGTTC TTAATGACCT TAGCCGCATT GTCTCTTGTA 
TTGAATGAAA AGAAAATCAA CAAAATGAAA AGAGGCGAAA TTTTCGATAT GGCCTTCACT 
GGTAGATACA TTATTTTGTT GATGGGTGTC TTTTCCATGT ACACAGGTTT CCTTTACAAC 
GATATCTTCT CTAAAACTAT GACTATTTTC AAGTCTGGTT GGAAATGGCC TGATCATTGG 
AAAAAAGGTG AGAGTATTAC TGCTACATCG GTGGGTACAT ACCCTATCGG TTTAGATTGG 
GCTTGGCATG GAACTGAAAA TGCTTTGTTA TTTTCTAATT CTTACAAAAT GAAACTATCA 
ATTTTAATGG GGTTCATCCA CATGACCTAT TCTTATTTCT TTTCGTTGGC TAACCACCTA 
TACTTTAACT CTATGATTGA TATCATCGGT AACTTTATTC CTGGTTTGCT ATTTATGCAA 
GGTATCTTTG GTTATCTTTC CGTTTGTATT GTTTACAAAT GGGCTGTTGA TTGGGTTAAG 
GACGGAAAGC CTGCTCCAGG TTTGTTAAAT ATGTTGATCA ACATGTTTTT ATCACCAGGA 
ACTATTGACG ATGAATTATA CCCTCATCAA GCAAAGGTCC AAGTGTTTTT GTTGTTGATG 
GCCTTGGTTT GTATTCCTTG GTTGCTATTG GTGAAGCCAT TACATTTCAA ATTCACTCAT 
AAAAAGAAAT CTCACGAACC ACTGCCATCG ACTGAAGCAG ATGCTAGTTC TGAAGATTTG 
GAAGCACAAC AATTAATTTC CGCGATGGAC GCCGATGACG CTGAAGAAGA AGAAGTTGGT 
TCTGGATCTC ATGGTGAAGA CTTTGGTGAT ATTATGATTC ATCAAGTTAT TCATACAATT 
GAATTCTGTT TGAATTGTGT TTCGCACACT GCATCCTATT TACGTTTATG GGCCTTATCA 
TTGGCACATG CTCAATTGTC TAGTGTTTTA TGGACAATGA CAATTCAAAT TGCCTTTGGA 
TTTAGAGGAT TTGTGGGTGT GTTTATGACG GTTGCACTTT TTGCCATGTG GTTCGCACTA 
ACATGTGCAG TTCTTGTTTT GATGGAAGGT ACATCTGCCA TGCTTCATTC CTTACGTTTG 
CACTGGGTTG AATCTATGTC CAAGTTTTTC GTGGGTGAAG GTTTACCATA CGAACCATTC 
GCATTTGAGT ATAAAGACAT GGAAGTCGCT GTTGCTAGTG CAAGCTCTTC CGCTTCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_014913.3
CDS	1..2523
Translation	MAEKEEAIFRSAEMALVQFYIPQEISRDSAYTLGQLGLVQFRDLNSKVRAFQRTFVNEIRRLDNVERQYRYFYSLLKKHDIKLYEGDTDKYLDGSGELYVPPSGSVIDDYVRNASYLEERLIQMEDATDQIEVQKNDLEQYRFILQSGDEFFLKGDNTDSTSYMDEDMIDANGENIAAAIGASVNYVTGVIARDKVATLEQILWRVLRGNLFFKTVEIEQPVYDVKTREYKHKNAFIVFSHGDLIIKRIRKIAESLDANLYDVDSSNEGRSQQLAKVNKNLSDLYTVLKTTSTTLESELYAIAKELDSWFQDVTREKAIFEILNKSNYDTNRKILIAEGWIPRDELATLQARLGEMIARLGIDVPSIIQVLDTNHTPPTFHRTNKFTAGFQSICDCYGIAQYREINAGLPTIVTFPFMFAIMFGDMGHGFLMTLAALSLVLNEKKINKMKRGEIFDMAFTGRYIILLMGVFSMYTGFLYNDIFSKTMTIFKSGWKWPDHWKKGESITATSVGTYPIGLDWAWHGTENALLFSNSYKMKLSILMGFIHMTYSYFFSLANHLYFNSMIDIIGNFIPGLLFMQGIFGYLSVCIVYKWAVDWVKDGKPAPGLLNMLINMFLSPGTIDDELYPHQAKVQVFLLLMALVCIPWLLLVKPLHFKFTHKKKSHEPLPSTEADASSEDLEAQQLISAMDADDAEEEEVGSGSHGEDFGDIMIHQVIHTIEFCLNCVSHTASYLRLWALSLAHAQLSSVLWTMTIQIAFGFRGFVGVFMTVALFAMWFALTCAVLVLMEGTSAMLHSLRLHWVESMSKFFVGEGLPYEPFAFEYKDMEVAVASASSSASS

Target ORF information:

RefSeq Version	NM_001183689.3
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C H(+)-transporting V0 sector ATPase subunit a (VPH1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001183689.3

ORF Insert Sequence:

ATGGCAGAGA AGGAGGAAGC GATTTTTCGC TCTGCTGAAA TGGCTTTAGT CCAATTCTAT 
ATTCCTCAAG AAATTTCAAG AGACTCTGCT TACACTTTAG GTCAATTGGG TCTTGTTCAA 
TTCCGTGACT TGAACTCTAA GGTGCGTGCG TTTCAAAGAA CTTTCGTGAA CGAAATTAGA 
AGACTGGATA ATGTAGAAAG ACAGTATCGT TACTTTTATT CTCTTTTGAA GAAACACGAT 
ATTAAGCTCT ACGAAGGAGA CACGGACAAA TATTTGGACG GCTCAGGTGA ATTGTACGTT 
CCACCAAGCG GTTCAGTGAT AGATGATTAT GTCCGGAACG CTTCATATTT GGAAGAAAGA 
TTGATTCAAA TGGAGGATGC AACCGATCAA ATTGAAGTCC AGAAAAATGA CTTGGAACAG 
TATCGCTTTA TTTTGCAGTC AGGTGATGAA TTTTTCTTGA AGGGTGATAA TACCGACAGC 
ACTTCCTATA TGGATGAAGA CATGATCGAC GCTAATGGGG AAAACATTGC TGCTGCTATC 
GGTGCTTCTG TAAACTATGT CACTGGTGTC ATTGCTAGAG ACAAAGTTGC CACCTTAGAA 
CAAATTCTTT GGAGAGTATT AAGAGGTAAC CTTTTCTTCA AAACTGTTGA AATTGAACAA 
CCTGTTTATG ATGTCAAAAC CAGGGAGTAT AAACATAAAA ATGCTTTTAT CGTATTTTCT 
CACGGTGATC TGATTATTAA AAGAATCAGA AAGATTGCGG AATCATTGGA TGCCAATCTT 
TACGATGTTG ACTCTTCCAA CGAGGGTAGA TCACAACAAT TGGCCAAGGT CAACAAGAAT 
TTGAGTGATT TGTACACAGT TTTGAAAACC ACTTCTACCA CTTTAGAAAG TGAATTATAT 
GCCATTGCCA AAGAATTGGA CTCTTGGTTC CAAGATGTTA CCCGTGAAAA GGCGATTTTT 
GAAATTTTGA ACAAGTCTAA CTATGATACC AATAGAAAGA TTTTGATTGC TGAAGGTTGG 
ATACCAAGAG ACGAATTGGC TACTTTGCAA GCTCGTCTTG GTGAAATGAT CGCAAGATTG 
GGTATTGATG TCCCATCCAT TATCCAAGTC CTGGATACAA ACCACACTCC ACCTACCTTC 
CACAGAACTA ACAAGTTTAC TGCTGGTTTC CAAAGTATCT GTGACTGTTA CGGTATTGCT 
CAGTACAGAG AAATCAATGC TGGTTTACCC ACAATTGTCA CTTTCCCTTT CATGTTTGCC 
ATCATGTTTG GTGATATGGG TCACGGGTTC TTAATGACCT TAGCCGCATT GTCTCTTGTA 
TTGAATGAAA AGAAAATCAA CAAAATGAAA AGAGGCGAAA TTTTCGATAT GGCCTTCACT 
GGTAGATACA TTATTTTGTT GATGGGTGTC TTTTCCATGT ACACAGGTTT CCTTTACAAC 
GATATCTTCT CTAAAACTAT GACTATTTTC AAGTCTGGTT GGAAATGGCC TGATCATTGG 
AAAAAAGGTG AGAGTATTAC TGCTACATCG GTGGGTACAT ACCCTATCGG TTTAGATTGG 
GCTTGGCATG GAACTGAAAA TGCTTTGTTA TTTTCTAATT CTTACAAAAT GAAACTATCA 
ATTTTAATGG GGTTCATCCA CATGACCTAT TCTTATTTCT TTTCGTTGGC TAACCACCTA 
TACTTTAACT CTATGATTGA TATCATCGGT AACTTTATTC CTGGTTTGCT ATTTATGCAA 
GGTATCTTTG GTTATCTTTC CGTTTGTATT GTTTACAAAT GGGCTGTTGA TTGGGTTAAG 
GACGGAAAGC CTGCTCCAGG TTTGTTAAAT ATGTTGATCA ACATGTTTTT ATCACCAGGA 
ACTATTGACG ATGAATTATA CCCTCATCAA GCAAAGGTCC AAGTGTTTTT GTTGTTGATG 
GCCTTGGTTT GTATTCCTTG GTTGCTATTG GTGAAGCCAT TACATTTCAA ATTCACTCAT 
AAAAAGAAAT CTCACGAACC ACTGCCATCG ACTGAAGCAG ATGCTAGTTC TGAAGATTTG 
GAAGCACAAC AATTAATTTC CGCGATGGAC GCCGATGACG CTGAAGAAGA AGAAGTTGGT 
TCTGGATCTC ATGGTGAAGA CTTTGGTGAT ATTATGATTC ATCAAGTTAT TCATACAATT 
GAATTCTGTT TGAATTGTGT TTCGCACACT GCATCCTATT TACGTTTATG GGCCTTATCA 
TTGGCACATG CTCAATTGTC TAGTGTTTTA TGGACAATGA CAATTCAAAT TGCCTTTGGA 
TTTAGAGGAT TTGTGGGTGT GTTTATGACG GTTGCACTTT TTGCCATGTG GTTCGCACTA 
ACATGTGCAG TTCTTGTTTT GATGGAAGGT ACATCTGCCA TGCTTCATTC CTTACGTTTG 
CACTGGGTTG AATCTATGTC CAAGTTTTTC GTGGGTGAAG GTTTACCATA CGAACCATTC 
GCATTTGAGT ATAAAGACAT GGAAGTCGCT GTTGCTAGTG CAAGCTCTTC CGCTTCAAGC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The nucleotide sequence of Saccharomyces cerevisiae chromosome XV.
Nature387(6632 Suppl)98-102(1997 May)
Dujon B,Albermann K,Aldea M,Alexandraki D,Ansorge W,Arino J,Benes V,Bohn C,Bolotin-Fukuhara M,Bordonn? R,Boyer J,Camasses A,Casamayor A,Casas C,Ch?ret G,Cziepluch C,Daignan-Fornier B,Dang DV,de Haan M,Delius H,Durand P,Fairhead C,Feldmann H,Gaillon L,Kleine K

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

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VPH1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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