Vha100-1 gene cDNA ORF clone, Drosophila melanogaster(fruit fly)

mRNA	Protein	Name
NM_170391.3	NP_733270.1	vacuolar H[+] ATPase 100kD subunit 1, isoform C
NM_170395.3	NP_733274.1	vacuolar H[+] ATPase 100kD subunit 1, isoform B
NM_170393.3	NP_733272.1	vacuolar H[+] ATPase 100kD subunit 1, isoform F
NM_143415.3	NP_651672.1	vacuolar H[+] ATPase 100kD subunit 1, isoform E
NM_170397.3	NP_733276.3	vacuolar H[+] ATPase 100kD subunit 1, isoform K
NM_170392.2	NP_733271.1	vacuolar H[+] ATPase 100kD subunit 1, isoform A
NM_170396.2	NP_733275.1	vacuolar H[+] ATPase 100kD subunit 1, isoform D
NM_001170297.1	NP_001163768.1	vacuolar H[+] ATPase 100kD subunit 1, isoform I
NM_170394.3	NP_733273.1	vacuolar H[+] ATPase 100kD subunit 1, isoform G
NM_001170298.2	NP_001163769.1	vacuolar H[+] ATPase 100kD subunit 1, isoform J

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OFa06463	NM_170392.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant A (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06463	NM_170393.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant F (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06464	NM_170397.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant K (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06464	NM_170395.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant B (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06464	NM_170396.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant D (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06465	NM_170391.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant C (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06465	NM_143415.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant E (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa06466	NM_170394.3 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant G (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08942	NM_001170297.1 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant I (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa08942	NM_001170298.2 Latest version!	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant J (Vha100-1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

Vha100-1 ( NM_170393.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733272.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant F (Vha100-1), mRNA.

Price & Availability↑

CloneID	OFa06463
Clone ID Related Accession (Same CDS sequence)	NM_170393.3 , NM_170392.2
Accession Version	NM_170393.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform F
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170393.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGATGGCCG ACAACCAAAA CGAGGACGAG CAGGCGCAGC TGCTGGGCGA GGAGGGTGTC 
CGGGCCAGCC AACCGGGCCA GAATTTGAAG CTTGGCTTCG TGGCTGGCGT CATCCTGAGG 
GAGCGACTGC CGGCCTTCGA GCGGATGTTG TGGCGCGCCT GCAGGGGTAA CGTCTTCCTG 
CGACAGGCGA TGATTGAGAC GCCGCTGGAG GATCCCACCA ATGGCGACCA GGTGCACAAG 
TCCGTGTTTA TAATCTTCTT CCAGGGTGAC CAGCTGAAGA CGCGCGTCAA GAAGATCTGC 
GAGGGCTTCC GGGCCACGCT CTATCCTTGC CCTGAAGCTC CAGCCGATCG GCGAGAGATG 
GCCATGGGTG TGATGACTCG CATCGAAGAT CTGAACACCG TGCTCGGCCA GACGCAGGAC 
CATCGCCATC GTGTGCTGGT CGCTGCGGCC AAGAATCTTA AGAACTGGTT CGTCAAGGTG 
CGCAAGATAA AGGCCATCTA CCATACGCTG AATCTCTTCA ATCTGGACGT GACCCAGAAG 
TGTCTGATCG CCGAGTGCTG GGTACCGCTG CTGGACATTG AAACCATCCA GCTGGCCTTG 
CGCCGCGGAA CCGAAAGATC GGGATCGTCG GTGCCGCCAA TTCTCAACCG GATGCAGACG 
TTCGAGAATC CACCGACCTA CAATCGAACG AATAAGTTTA CCAAGGCCTT TCAGGCATTG 
ATCGATGCCT ATGGCGTGGC CAGCTATCGG GAGATGAATC CGGCACCCTA CACGATCATC 
ACCTTCCCCT TCCTGTTCGC CGTGATGTTT GGAGATTTGG GCCACGGAGC TATCATGGCG 
CTCTTTGGTC TCTGGATGAT TCGAAAGGAG AAGGGACTGG CGGCTCAGAA GACGGACAAC 
GAGATCTGGA ACATATTCTT TGGCGGACGG TACATCATCT TCCTCATGGG CGTCTTCTCC 
ATGTACACGG GTCTTATATA CAACGATATA TTCTCCAAGT CGCTGAATAT CTTTGGATCG 
CATTGGCACT TATCCTACAA CAAGTCCACC GTGATGGAGA ACAAGTTCCT GCAGTTGAGC 
CCGAAAGGCG ACTACGAGGG CGCTCCGTAT CCGTTCGGCA TGGATCCCAT TTGGCAGGTG 
GCGGGAGCCA ATAAGATCAT CTTCCACAAC GCTTACAAGA TGAAGATCTC GATCATTTTC 
GGTGTTATTC ACATGATCTT CGGTGTGGTG ATGAGCTGGC ACAATCATAC GTATTTCAGG 
AACAGGATCT CCCTGCTCTA CGAGTTCATT CCCCAGCTGG TCTTCCTGCT GCTGCTTTTC 
TTCTACATGG TTTTGTTGAT GTTCATCAAG TGGATCAAGT TTGCCGCCAC CAATGATAAG 
CCCTACTCCG AAGCCTGTGC GCCTTCAATC CTGATCACCT TTATCGACAT GGTGCTTTTT 
AACACGCCCA AGCCACCTCC GGAGAACTGT GAAACCTATA TGTTCATGGG CCAGCACTTT 
ATCCAGGTGT TATTCGTCCT GGTTGCCGTC GGCTGCATTC CCGTAATGTT GCTGGCCAAG 
CCGCTGCTCA TCATGCAGGC CCGCAAGCAG GCGAACGTAC AGCCCATTGC AGGAGCAACT 
TCAGATGCAG AAGCCGGCGG CGTGTCCAAT AGCGGCTCAC ACGGTGGTGG CGGTGGCCAT 
GAGGAGGAAG AGGAGCTCTC TGAGATCTTC ATTCACCAGA GCATCCACAC CATCGAGTAC 
GTCCTAGGTT CGGTATCCCA CACCGCTTCC TATCTGCGAT TGTGGGCGCT TTCCTTGGCC 
CATGCCCAGT TGGCTGAGGT GCTGTGGACC ATGGTCCTAT CGATTGGCTT GAAGCAGGAA 
GGGCCGGTGG GTGGCATTGT ATTGACCTGC GTGTTTGCCT TCTGGGCCAT TCTCACCGTT 
GGCATTCTGG TACTCATGGA GGGCTTGTCT GCCTTCCTGC ACACCCTGCG TCTCCATTGG 
GTCGAGTTCC AGAGCAAGTT CTACAAGGGC CAGGGTTACG CCTTCCAACC CTTCTCGTTT 
GATGCCATCA TAGAAAACGG AGCTGCCGCC GCCGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733272.1
CDS	103..2604
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEMADNQNEDEQAQLLGEEGVRASQPGQNLKLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_170393.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant F (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170393.3

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGATGGCCG ACAACCAAAA CGAGGACGAG CAGGCGCAGC TGCTGGGCGA GGAGGGTGTC 
CGGGCCAGCC AACCGGGCCA GAATTTGAAG CTTGGCTTCG TGGCTGGCGT CATCCTGAGG 
GAGCGACTGC CGGCCTTCGA GCGGATGTTG TGGCGCGCCT GCAGGGGTAA CGTCTTCCTG 
CGACAGGCGA TGATTGAGAC GCCGCTGGAG GATCCCACCA ATGGCGACCA GGTGCACAAG 
TCCGTGTTTA TAATCTTCTT CCAGGGTGAC CAGCTGAAGA CGCGCGTCAA GAAGATCTGC 
GAGGGCTTCC GGGCCACGCT CTATCCTTGC CCTGAAGCTC CAGCCGATCG GCGAGAGATG 
GCCATGGGTG TGATGACTCG CATCGAAGAT CTGAACACCG TGCTCGGCCA GACGCAGGAC 
CATCGCCATC GTGTGCTGGT CGCTGCGGCC AAGAATCTTA AGAACTGGTT CGTCAAGGTG 
CGCAAGATAA AGGCCATCTA CCATACGCTG AATCTCTTCA ATCTGGACGT GACCCAGAAG 
TGTCTGATCG CCGAGTGCTG GGTACCGCTG CTGGACATTG AAACCATCCA GCTGGCCTTG 
CGCCGCGGAA CCGAAAGATC GGGATCGTCG GTGCCGCCAA TTCTCAACCG GATGCAGACG 
TTCGAGAATC CACCGACCTA CAATCGAACG AATAAGTTTA CCAAGGCCTT TCAGGCATTG 
ATCGATGCCT ATGGCGTGGC CAGCTATCGG GAGATGAATC CGGCACCCTA CACGATCATC 
ACCTTCCCCT TCCTGTTCGC CGTGATGTTT GGAGATTTGG GCCACGGAGC TATCATGGCG 
CTCTTTGGTC TCTGGATGAT TCGAAAGGAG AAGGGACTGG CGGCTCAGAA GACGGACAAC 
GAGATCTGGA ACATATTCTT TGGCGGACGG TACATCATCT TCCTCATGGG CGTCTTCTCC 
ATGTACACGG GTCTTATATA CAACGATATA TTCTCCAAGT CGCTGAATAT CTTTGGATCG 
CATTGGCACT TATCCTACAA CAAGTCCACC GTGATGGAGA ACAAGTTCCT GCAGTTGAGC 
CCGAAAGGCG ACTACGAGGG CGCTCCGTAT CCGTTCGGCA TGGATCCCAT TTGGCAGGTG 
GCGGGAGCCA ATAAGATCAT CTTCCACAAC GCTTACAAGA TGAAGATCTC GATCATTTTC 
GGTGTTATTC ACATGATCTT CGGTGTGGTG ATGAGCTGGC ACAATCATAC GTATTTCAGG 
AACAGGATCT CCCTGCTCTA CGAGTTCATT CCCCAGCTGG TCTTCCTGCT GCTGCTTTTC 
TTCTACATGG TTTTGTTGAT GTTCATCAAG TGGATCAAGT TTGCCGCCAC CAATGATAAG 
CCCTACTCCG AAGCCTGTGC GCCTTCAATC CTGATCACCT TTATCGACAT GGTGCTTTTT 
AACACGCCCA AGCCACCTCC GGAGAACTGT GAAACCTATA TGTTCATGGG CCAGCACTTT 
ATCCAGGTGT TATTCGTCCT GGTTGCCGTC GGCTGCATTC CCGTAATGTT GCTGGCCAAG 
CCGCTGCTCA TCATGCAGGC CCGCAAGCAG GCGAACGTAC AGCCCATTGC AGGAGCAACT 
TCAGATGCAG AAGCCGGCGG CGTGTCCAAT AGCGGCTCAC ACGGTGGTGG CGGTGGCCAT 
GAGGAGGAAG AGGAGCTCTC TGAGATCTTC ATTCACCAGA GCATCCACAC CATCGAGTAC 
GTCCTAGGTT CGGTATCCCA CACCGCTTCC TATCTGCGAT TGTGGGCGCT TTCCTTGGCC 
CATGCCCAGT TGGCTGAGGT GCTGTGGACC ATGGTCCTAT CGATTGGCTT GAAGCAGGAA 
GGGCCGGTGG GTGGCATTGT ATTGACCTGC GTGTTTGCCT TCTGGGCCAT TCTCACCGTT 
GGCATTCTGG TACTCATGGA GGGCTTGTCT GCCTTCCTGC ACACCCTGCG TCTCCATTGG 
GTCGAGTTCC AGAGCAAGTT CTACAAGGGC CAGGGTTACG CCTTCCAACC CTTCTCGTTT 
GATGCCATCA TAGAAAACGG AGCTGCCGCC GCCGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_170392.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733271.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant A (Vha100-1), mRNA.

Price & Availability↑

CloneID	OFa06463
Clone ID Related Accession (Same CDS sequence)	NM_170393.3 , NM_170392.2
Accession Version	NM_170392.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2502bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform A
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_170392.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGATGGCCG ACAACCAAAA CGAGGACGAG CAGGCGCAGC TGCTGGGCGA GGAGGGTGTC 
CGGGCCAGCC AACCGGGCCA GAATTTGAAG CTTGGCTTCG TGGCTGGCGT CATCCTGAGG 
GAGCGACTGC CGGCCTTCGA GCGGATGTTG TGGCGCGCCT GCAGGGGTAA CGTCTTCCTG 
CGACAGGCGA TGATTGAGAC GCCGCTGGAG GATCCCACCA ATGGCGACCA GGTGCACAAG 
TCCGTGTTTA TAATCTTCTT CCAGGGTGAC CAGCTGAAGA CGCGCGTCAA GAAGATCTGC 
GAGGGCTTCC GGGCCACGCT CTATCCTTGC CCTGAAGCTC CAGCCGATCG GCGAGAGATG 
GCCATGGGTG TGATGACTCG CATCGAAGAT CTGAACACCG TGCTCGGCCA GACGCAGGAC 
CATCGCCATC GTGTGCTGGT CGCTGCGGCC AAGAATCTTA AGAACTGGTT CGTCAAGGTG 
CGCAAGATAA AGGCCATCTA CCATACGCTG AATCTCTTCA ATCTGGACGT GACCCAGAAG 
TGTCTGATCG CCGAGTGCTG GGTACCGCTG CTGGACATTG AAACCATCCA GCTGGCCTTG 
CGCCGCGGAA CCGAAAGATC GGGATCGTCG GTGCCGCCAA TTCTCAACCG GATGCAGACG 
TTCGAGAATC CACCGACCTA CAATCGAACG AATAAGTTTA CCAAGGCCTT TCAGGCATTG 
ATCGATGCCT ATGGCGTGGC CAGCTATCGG GAGATGAATC CGGCACCCTA CACGATCATC 
ACCTTCCCCT TCCTGTTCGC CGTGATGTTT GGAGATTTGG GCCACGGAGC TATCATGGCG 
CTCTTTGGTC TCTGGATGAT TCGAAAGGAG AAGGGACTGG CGGCTCAGAA GACGGACAAC 
GAGATCTGGA ACATATTCTT TGGCGGACGG TACATCATCT TCCTCATGGG CGTCTTCTCC 
ATGTACACGG GTCTTATATA CAACGATATA TTCTCCAAGT CGCTGAATAT CTTTGGATCG 
CATTGGCACT TATCCTACAA CAAGTCCACC GTGATGGAGA ACAAGTTCCT GCAGTTGAGC 
CCGAAAGGCG ACTACGAGGG CGCTCCGTAT CCGTTCGGCA TGGATCCCAT TTGGCAGGTG 
GCGGGAGCCA ATAAGATCAT CTTCCACAAC GCTTACAAGA TGAAGATCTC GATCATTTTC 
GGTGTTATTC ACATGATCTT CGGTGTGGTG ATGAGCTGGC ACAATCATAC GTATTTCAGG 
AACAGGATCT CCCTGCTCTA CGAGTTCATT CCCCAGCTGG TCTTCCTGCT GCTGCTTTTC 
TTCTACATGG TTTTGTTGAT GTTCATCAAG TGGATCAAGT TTGCCGCCAC CAATGATAAG 
CCCTACTCCG AAGCCTGTGC GCCTTCAATC CTGATCACCT TTATCGACAT GGTGCTTTTT 
AACACGCCCA AGCCACCTCC GGAGAACTGT GAAACCTATA TGTTCATGGG CCAGCACTTT 
ATCCAGGTGT TATTCGTCCT GGTTGCCGTC GGCTGCATTC CCGTAATGTT GCTGGCCAAG 
CCGCTGCTCA TCATGCAGGC CCGCAAGCAG GCGAACGTAC AGCCCATTGC AGGAGCAACT 
TCAGATGCAG AAGCCGGCGG CGTGTCCAAT AGCGGCTCAC ACGGTGGTGG CGGTGGCCAT 
GAGGAGGAAG AGGAGCTCTC TGAGATCTTC ATTCACCAGA GCATCCACAC CATCGAGTAC 
GTCCTAGGTT CGGTATCCCA CACCGCTTCC TATCTGCGAT TGTGGGCGCT TTCCTTGGCC 
CATGCCCAGT TGGCTGAGGT GCTGTGGACC ATGGTCCTAT CGATTGGCTT GAAGCAGGAA 
GGGCCGGTGG GTGGCATTGT ATTGACCTGC GTGTTTGCCT TCTGGGCCAT TCTCACCGTT 
GGCATTCTGG TACTCATGGA GGGCTTGTCT GCCTTCCTGC ACACCCTGCG TCTCCATTGG 
GTCGAGTTCC AGAGCAAGTT CTACAAGGGC CAGGGTTACG CCTTCCAACC CTTCTCGTTT 
GATGCCATCA TAGAAAACGG AGCTGCCGCC GCCGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733271.1
CDS	205..2706
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEMADNQNEDEQAQLLGEEGVRASQPGQNLKLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_170392.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant A (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170392.2

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGATGGCCG ACAACCAAAA CGAGGACGAG CAGGCGCAGC TGCTGGGCGA GGAGGGTGTC 
CGGGCCAGCC AACCGGGCCA GAATTTGAAG CTTGGCTTCG TGGCTGGCGT CATCCTGAGG 
GAGCGACTGC CGGCCTTCGA GCGGATGTTG TGGCGCGCCT GCAGGGGTAA CGTCTTCCTG 
CGACAGGCGA TGATTGAGAC GCCGCTGGAG GATCCCACCA ATGGCGACCA GGTGCACAAG 
TCCGTGTTTA TAATCTTCTT CCAGGGTGAC CAGCTGAAGA CGCGCGTCAA GAAGATCTGC 
GAGGGCTTCC GGGCCACGCT CTATCCTTGC CCTGAAGCTC CAGCCGATCG GCGAGAGATG 
GCCATGGGTG TGATGACTCG CATCGAAGAT CTGAACACCG TGCTCGGCCA GACGCAGGAC 
CATCGCCATC GTGTGCTGGT CGCTGCGGCC AAGAATCTTA AGAACTGGTT CGTCAAGGTG 
CGCAAGATAA AGGCCATCTA CCATACGCTG AATCTCTTCA ATCTGGACGT GACCCAGAAG 
TGTCTGATCG CCGAGTGCTG GGTACCGCTG CTGGACATTG AAACCATCCA GCTGGCCTTG 
CGCCGCGGAA CCGAAAGATC GGGATCGTCG GTGCCGCCAA TTCTCAACCG GATGCAGACG 
TTCGAGAATC CACCGACCTA CAATCGAACG AATAAGTTTA CCAAGGCCTT TCAGGCATTG 
ATCGATGCCT ATGGCGTGGC CAGCTATCGG GAGATGAATC CGGCACCCTA CACGATCATC 
ACCTTCCCCT TCCTGTTCGC CGTGATGTTT GGAGATTTGG GCCACGGAGC TATCATGGCG 
CTCTTTGGTC TCTGGATGAT TCGAAAGGAG AAGGGACTGG CGGCTCAGAA GACGGACAAC 
GAGATCTGGA ACATATTCTT TGGCGGACGG TACATCATCT TCCTCATGGG CGTCTTCTCC 
ATGTACACGG GTCTTATATA CAACGATATA TTCTCCAAGT CGCTGAATAT CTTTGGATCG 
CATTGGCACT TATCCTACAA CAAGTCCACC GTGATGGAGA ACAAGTTCCT GCAGTTGAGC 
CCGAAAGGCG ACTACGAGGG CGCTCCGTAT CCGTTCGGCA TGGATCCCAT TTGGCAGGTG 
GCGGGAGCCA ATAAGATCAT CTTCCACAAC GCTTACAAGA TGAAGATCTC GATCATTTTC 
GGTGTTATTC ACATGATCTT CGGTGTGGTG ATGAGCTGGC ACAATCATAC GTATTTCAGG 
AACAGGATCT CCCTGCTCTA CGAGTTCATT CCCCAGCTGG TCTTCCTGCT GCTGCTTTTC 
TTCTACATGG TTTTGTTGAT GTTCATCAAG TGGATCAAGT TTGCCGCCAC CAATGATAAG 
CCCTACTCCG AAGCCTGTGC GCCTTCAATC CTGATCACCT TTATCGACAT GGTGCTTTTT 
AACACGCCCA AGCCACCTCC GGAGAACTGT GAAACCTATA TGTTCATGGG CCAGCACTTT 
ATCCAGGTGT TATTCGTCCT GGTTGCCGTC GGCTGCATTC CCGTAATGTT GCTGGCCAAG 
CCGCTGCTCA TCATGCAGGC CCGCAAGCAG GCGAACGTAC AGCCCATTGC AGGAGCAACT 
TCAGATGCAG AAGCCGGCGG CGTGTCCAAT AGCGGCTCAC ACGGTGGTGG CGGTGGCCAT 
GAGGAGGAAG AGGAGCTCTC TGAGATCTTC ATTCACCAGA GCATCCACAC CATCGAGTAC 
GTCCTAGGTT CGGTATCCCA CACCGCTTCC TATCTGCGAT TGTGGGCGCT TTCCTTGGCC 
CATGCCCAGT TGGCTGAGGT GCTGTGGACC ATGGTCCTAT CGATTGGCTT GAAGCAGGAA 
GGGCCGGTGG GTGGCATTGT ATTGACCTGC GTGTTTGCCT TCTGGGCCAT TCTCACCGTT 
GGCATTCTGG TACTCATGGA GGGCTTGTCT GCCTTCCTGC ACACCCTGCG TCTCCATTGG 
GTCGAGTTCC AGAGCAAGTT CTACAAGGGC CAGGGTTACG CCTTCCAACC CTTCTCGTTT 
GATGCCATCA TAGAAAACGG AGCTGCCGCC GCCGAGGAGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_170395.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733274.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant B (Vha100-1), mRNA.

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CloneID	OFa06464
Clone ID Related Accession (Same CDS sequence)	NM_170395.3 , NM_170397.3 , NM_170396.2
Accession Version	NM_170395.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170395.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGCTTCGT GGCTGGCGTC 
ATCCTGAGGG AGCGACTGCC GGCCTTCGAG CGGATGTTGT GGCGCGCCTG CAGGGGTAAC 
GTCTTCCTGC GACAGGCGAT GATTGAGACG CCGCTGGAGG ATCCCACCAA TGGCGACCAG 
GTGCACAAGT CCGTGTTTAT AATCTTCTTC CAGGGTGACC AGCTGAAGAC GCGCGTCAAG 
AAGATCTGCG AGGGCTTCCG GGCCACGCTC TATCCTTGCC CTGAAGCTCC AGCCGATCGG 
CGAGAGATGG CCATGGGTGT GATGACTCGC ATCGAAGATC TGAACACCGT GCTCGGCCAG 
ACGCAGGACC ATCGCCATCG TGTGCTGGTC GCTGCGGCCA AGAATCTTAA GAACTGGTTC 
GTCAAGGTGC GCAAGATAAA GGCCATCTAC CATACGCTGA ATCTCTTCAA TCTGGACGTG 
ACCCAGAAGT GTCTGATCGC CGAGTGCTGG GTACCGCTGC TGGACATTGA AACCATCCAG 
CTGGCCTTGC GCCGCGGAAC CGAAAGATCG GGATCGTCGG TGCCGCCAAT TCTCAACCGG 
ATGCAGACGT TCGAGAATCC ACCGACCTAC AATCGAACGA ATAAGTTTAC CAAGGCCTTT 
CAGGCATTGA TCGATGCCTA TGGCGTGGCC AGCTATCGGG AGATGAATCC GGCACCCTAC 
ACGATCATCA CCTTCCCCTT CCTGTTCGCC GTGATGTTTG GAGATTTGGG CCACGGAGCT 
ATCATGGCGC TCTTTGGTCT CTGGATGATT CGAAAGGAGA AGGGACTGGC GGCTCAGAAG 
ACGGACAACG AGATCTGGAA CATATTCTTT GGCGGACGGT ACATCATCTT CCTCATGGGC 
GTCTTCTCCA TGTACACGGG TCTTATATAC AACGATATAT TCTCCAAGTC GCTGAATATC 
TTTGGATCGC ATTGGCACTT ATCCTACAAC AAGTCCACCG TGATGGAGAA CAAGTTCCTG 
CAGTTGAGCC CGAAAGGCGA CTACGAGGGC GCTCCGTATC CGTTCGGCAT GGATCCCATT 
TGGCAGGTGG CGGGAGCCAA TAAGATCATC TTCCACAACG CTTACAAGAT GAAGATCTCG 
ATCATTTTCG GTGTTATTCA CATGATCTTC GGTGTGGTGA TGAGCTGGCA CAATCATACG 
TATTTCAGGA ACAGGATCTC CCTGCTCTAC GAGTTCATTC CCCAGCTGGT CTTCCTGCTG 
CTGCTTTTCT TCTACATGGT TTTGTTGATG TTCATCAAGT GGATCAAGTT TGCCGCCACC 
AATGATAAGC CCTACTCCGA AGCCTGTGCG CCTTCAATCC TGATCACCTT TATCGACATG 
GTGCTTTTTA ACACGCCCAA GCCACCTCCG GAGAACTGTG AAACCTATAT GTTCATGGGC 
CAGCACTTTA TCCAGGTGTT ATTCGTCCTG GTTGCCGTCG GCTGCATTCC CGTAATGTTG 
CTGGCCAAGC CGCTGCTCAT CATGCAGGCC CGCAAGCAGG CGAACGTACA GCCCATTGCA 
GGAGCAACTT CAGATGCAGA AGCCGGCGGC GTGTCCAATA GCGGCTCACA CGGTGGTGGC 
GGTGGCCATG AGGAGGAAGA GGAGCTCTCT GAGATCTTCA TTCACCAGAG CATCCACACC 
ATCGAGTACG TCCTAGGTTC GGTATCCCAC ACCGCTTCCT ATCTGCGATT GTGGGCGCTT 
TCCTTGGCCC ATGCCCAGTT GGCTGAGGTG CTGTGGACCA TGGTCCTATC GATTGGCTTG 
AAGCAGGAAG GGCCGGTGGG TGGCATTGTA TTGACCTGCG TGTTTGCCTT CTGGGCCATT 
CTCACCGTTG GCATTCTGGT ACTCATGGAG GGCTTGTCTG CCTTCCTGCA CACCCTGCGT 
CTCCATTGGG TCGAGTTCCA GAGCAAGTTC TACAAGGGCC AGGGTTACGC CTTCCAACCC 
TTCTCGTTTG ATGCCATCAT AGAAAACGGA GCTGCCGCCG CCGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733274.1
CDS	103..2613
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEQEGGVNQTTESMTRALITDEARTAGASMGPVQLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_170395.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant B (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170395.3

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGCTTCGT GGCTGGCGTC 
ATCCTGAGGG AGCGACTGCC GGCCTTCGAG CGGATGTTGT GGCGCGCCTG CAGGGGTAAC 
GTCTTCCTGC GACAGGCGAT GATTGAGACG CCGCTGGAGG ATCCCACCAA TGGCGACCAG 
GTGCACAAGT CCGTGTTTAT AATCTTCTTC CAGGGTGACC AGCTGAAGAC GCGCGTCAAG 
AAGATCTGCG AGGGCTTCCG GGCCACGCTC TATCCTTGCC CTGAAGCTCC AGCCGATCGG 
CGAGAGATGG CCATGGGTGT GATGACTCGC ATCGAAGATC TGAACACCGT GCTCGGCCAG 
ACGCAGGACC ATCGCCATCG TGTGCTGGTC GCTGCGGCCA AGAATCTTAA GAACTGGTTC 
GTCAAGGTGC GCAAGATAAA GGCCATCTAC CATACGCTGA ATCTCTTCAA TCTGGACGTG 
ACCCAGAAGT GTCTGATCGC CGAGTGCTGG GTACCGCTGC TGGACATTGA AACCATCCAG 
CTGGCCTTGC GCCGCGGAAC CGAAAGATCG GGATCGTCGG TGCCGCCAAT TCTCAACCGG 
ATGCAGACGT TCGAGAATCC ACCGACCTAC AATCGAACGA ATAAGTTTAC CAAGGCCTTT 
CAGGCATTGA TCGATGCCTA TGGCGTGGCC AGCTATCGGG AGATGAATCC GGCACCCTAC 
ACGATCATCA CCTTCCCCTT CCTGTTCGCC GTGATGTTTG GAGATTTGGG CCACGGAGCT 
ATCATGGCGC TCTTTGGTCT CTGGATGATT CGAAAGGAGA AGGGACTGGC GGCTCAGAAG 
ACGGACAACG AGATCTGGAA CATATTCTTT GGCGGACGGT ACATCATCTT CCTCATGGGC 
GTCTTCTCCA TGTACACGGG TCTTATATAC AACGATATAT TCTCCAAGTC GCTGAATATC 
TTTGGATCGC ATTGGCACTT ATCCTACAAC AAGTCCACCG TGATGGAGAA CAAGTTCCTG 
CAGTTGAGCC CGAAAGGCGA CTACGAGGGC GCTCCGTATC CGTTCGGCAT GGATCCCATT 
TGGCAGGTGG CGGGAGCCAA TAAGATCATC TTCCACAACG CTTACAAGAT GAAGATCTCG 
ATCATTTTCG GTGTTATTCA CATGATCTTC GGTGTGGTGA TGAGCTGGCA CAATCATACG 
TATTTCAGGA ACAGGATCTC CCTGCTCTAC GAGTTCATTC CCCAGCTGGT CTTCCTGCTG 
CTGCTTTTCT TCTACATGGT TTTGTTGATG TTCATCAAGT GGATCAAGTT TGCCGCCACC 
AATGATAAGC CCTACTCCGA AGCCTGTGCG CCTTCAATCC TGATCACCTT TATCGACATG 
GTGCTTTTTA ACACGCCCAA GCCACCTCCG GAGAACTGTG AAACCTATAT GTTCATGGGC 
CAGCACTTTA TCCAGGTGTT ATTCGTCCTG GTTGCCGTCG GCTGCATTCC CGTAATGTTG 
CTGGCCAAGC CGCTGCTCAT CATGCAGGCC CGCAAGCAGG CGAACGTACA GCCCATTGCA 
GGAGCAACTT CAGATGCAGA AGCCGGCGGC GTGTCCAATA GCGGCTCACA CGGTGGTGGC 
GGTGGCCATG AGGAGGAAGA GGAGCTCTCT GAGATCTTCA TTCACCAGAG CATCCACACC 
ATCGAGTACG TCCTAGGTTC GGTATCCCAC ACCGCTTCCT ATCTGCGATT GTGGGCGCTT 
TCCTTGGCCC ATGCCCAGTT GGCTGAGGTG CTGTGGACCA TGGTCCTATC GATTGGCTTG 
AAGCAGGAAG GGCCGGTGGG TGGCATTGTA TTGACCTGCG TGTTTGCCTT CTGGGCCATT 
CTCACCGTTG GCATTCTGGT ACTCATGGAG GGCTTGTCTG CCTTCCTGCA CACCCTGCGT 
CTCCATTGGG TCGAGTTCCA GAGCAAGTTC TACAAGGGCC AGGGTTACGC CTTCCAACCC 
TTCTCGTTTG ATGCCATCAT AGAAAACGGA GCTGCCGCCG CCGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_170397.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733276.3 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant K (Vha100-1), mRNA.

Price & Availability↑

CloneID	OFa06464
Clone ID Related Accession (Same CDS sequence)	NM_170395.3 , NM_170397.3 , NM_170396.2
Accession Version	NM_170397.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform K
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170397.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGCTTCGT GGCTGGCGTC 
ATCCTGAGGG AGCGACTGCC GGCCTTCGAG CGGATGTTGT GGCGCGCCTG CAGGGGTAAC 
GTCTTCCTGC GACAGGCGAT GATTGAGACG CCGCTGGAGG ATCCCACCAA TGGCGACCAG 
GTGCACAAGT CCGTGTTTAT AATCTTCTTC CAGGGTGACC AGCTGAAGAC GCGCGTCAAG 
AAGATCTGCG AGGGCTTCCG GGCCACGCTC TATCCTTGCC CTGAAGCTCC AGCCGATCGG 
CGAGAGATGG CCATGGGTGT GATGACTCGC ATCGAAGATC TGAACACCGT GCTCGGCCAG 
ACGCAGGACC ATCGCCATCG TGTGCTGGTC GCTGCGGCCA AGAATCTTAA GAACTGGTTC 
GTCAAGGTGC GCAAGATAAA GGCCATCTAC CATACGCTGA ATCTCTTCAA TCTGGACGTG 
ACCCAGAAGT GTCTGATCGC CGAGTGCTGG GTACCGCTGC TGGACATTGA AACCATCCAG 
CTGGCCTTGC GCCGCGGAAC CGAAAGATCG GGATCGTCGG TGCCGCCAAT TCTCAACCGG 
ATGCAGACGT TCGAGAATCC ACCGACCTAC AATCGAACGA ATAAGTTTAC CAAGGCCTTT 
CAGGCATTGA TCGATGCCTA TGGCGTGGCC AGCTATCGGG AGATGAATCC GGCACCCTAC 
ACGATCATCA CCTTCCCCTT CCTGTTCGCC GTGATGTTTG GAGATTTGGG CCACGGAGCT 
ATCATGGCGC TCTTTGGTCT CTGGATGATT CGAAAGGAGA AGGGACTGGC GGCTCAGAAG 
ACGGACAACG AGATCTGGAA CATATTCTTT GGCGGACGGT ACATCATCTT CCTCATGGGC 
GTCTTCTCCA TGTACACGGG TCTTATATAC AACGATATAT TCTCCAAGTC GCTGAATATC 
TTTGGATCGC ATTGGCACTT ATCCTACAAC AAGTCCACCG TGATGGAGAA CAAGTTCCTG 
CAGTTGAGCC CGAAAGGCGA CTACGAGGGC GCTCCGTATC CGTTCGGCAT GGATCCCATT 
TGGCAGGTGG CGGGAGCCAA TAAGATCATC TTCCACAACG CTTACAAGAT GAAGATCTCG 
ATCATTTTCG GTGTTATTCA CATGATCTTC GGTGTGGTGA TGAGCTGGCA CAATCATACG 
TATTTCAGGA ACAGGATCTC CCTGCTCTAC GAGTTCATTC CCCAGCTGGT CTTCCTGCTG 
CTGCTTTTCT TCTACATGGT TTTGTTGATG TTCATCAAGT GGATCAAGTT TGCCGCCACC 
AATGATAAGC CCTACTCCGA AGCCTGTGCG CCTTCAATCC TGATCACCTT TATCGACATG 
GTGCTTTTTA ACACGCCCAA GCCACCTCCG GAGAACTGTG AAACCTATAT GTTCATGGGC 
CAGCACTTTA TCCAGGTGTT ATTCGTCCTG GTTGCCGTCG GCTGCATTCC CGTAATGTTG 
CTGGCCAAGC CGCTGCTCAT CATGCAGGCC CGCAAGCAGG CGAACGTACA GCCCATTGCA 
GGAGCAACTT CAGATGCAGA AGCCGGCGGC GTGTCCAATA GCGGCTCACA CGGTGGTGGC 
GGTGGCCATG AGGAGGAAGA GGAGCTCTCT GAGATCTTCA TTCACCAGAG CATCCACACC 
ATCGAGTACG TCCTAGGTTC GGTATCCCAC ACCGCTTCCT ATCTGCGATT GTGGGCGCTT 
TCCTTGGCCC ATGCCCAGTT GGCTGAGGTG CTGTGGACCA TGGTCCTATC GATTGGCTTG 
AAGCAGGAAG GGCCGGTGGG TGGCATTGTA TTGACCTGCG TGTTTGCCTT CTGGGCCATT 
CTCACCGTTG GCATTCTGGT ACTCATGGAG GGCTTGTCTG CCTTCCTGCA CACCCTGCGT 
CTCCATTGGG TCGAGTTCCA GAGCAAGTTC TACAAGGGCC AGGGTTACGC CTTCCAACCC 
TTCTCGTTTG ATGCCATCAT AGAAAACGGA GCTGCCGCCG CCGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733276.3
CDS	103..2613
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEQEGGVNQTTESMTRALITDEARTAGASMGPVQLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_170397.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant K (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170397.3

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGCTTCGT GGCTGGCGTC 
ATCCTGAGGG AGCGACTGCC GGCCTTCGAG CGGATGTTGT GGCGCGCCTG CAGGGGTAAC 
GTCTTCCTGC GACAGGCGAT GATTGAGACG CCGCTGGAGG ATCCCACCAA TGGCGACCAG 
GTGCACAAGT CCGTGTTTAT AATCTTCTTC CAGGGTGACC AGCTGAAGAC GCGCGTCAAG 
AAGATCTGCG AGGGCTTCCG GGCCACGCTC TATCCTTGCC CTGAAGCTCC AGCCGATCGG 
CGAGAGATGG CCATGGGTGT GATGACTCGC ATCGAAGATC TGAACACCGT GCTCGGCCAG 
ACGCAGGACC ATCGCCATCG TGTGCTGGTC GCTGCGGCCA AGAATCTTAA GAACTGGTTC 
GTCAAGGTGC GCAAGATAAA GGCCATCTAC CATACGCTGA ATCTCTTCAA TCTGGACGTG 
ACCCAGAAGT GTCTGATCGC CGAGTGCTGG GTACCGCTGC TGGACATTGA AACCATCCAG 
CTGGCCTTGC GCCGCGGAAC CGAAAGATCG GGATCGTCGG TGCCGCCAAT TCTCAACCGG 
ATGCAGACGT TCGAGAATCC ACCGACCTAC AATCGAACGA ATAAGTTTAC CAAGGCCTTT 
CAGGCATTGA TCGATGCCTA TGGCGTGGCC AGCTATCGGG AGATGAATCC GGCACCCTAC 
ACGATCATCA CCTTCCCCTT CCTGTTCGCC GTGATGTTTG GAGATTTGGG CCACGGAGCT 
ATCATGGCGC TCTTTGGTCT CTGGATGATT CGAAAGGAGA AGGGACTGGC GGCTCAGAAG 
ACGGACAACG AGATCTGGAA CATATTCTTT GGCGGACGGT ACATCATCTT CCTCATGGGC 
GTCTTCTCCA TGTACACGGG TCTTATATAC AACGATATAT TCTCCAAGTC GCTGAATATC 
TTTGGATCGC ATTGGCACTT ATCCTACAAC AAGTCCACCG TGATGGAGAA CAAGTTCCTG 
CAGTTGAGCC CGAAAGGCGA CTACGAGGGC GCTCCGTATC CGTTCGGCAT GGATCCCATT 
TGGCAGGTGG CGGGAGCCAA TAAGATCATC TTCCACAACG CTTACAAGAT GAAGATCTCG 
ATCATTTTCG GTGTTATTCA CATGATCTTC GGTGTGGTGA TGAGCTGGCA CAATCATACG 
TATTTCAGGA ACAGGATCTC CCTGCTCTAC GAGTTCATTC CCCAGCTGGT CTTCCTGCTG 
CTGCTTTTCT TCTACATGGT TTTGTTGATG TTCATCAAGT GGATCAAGTT TGCCGCCACC 
AATGATAAGC CCTACTCCGA AGCCTGTGCG CCTTCAATCC TGATCACCTT TATCGACATG 
GTGCTTTTTA ACACGCCCAA GCCACCTCCG GAGAACTGTG AAACCTATAT GTTCATGGGC 
CAGCACTTTA TCCAGGTGTT ATTCGTCCTG GTTGCCGTCG GCTGCATTCC CGTAATGTTG 
CTGGCCAAGC CGCTGCTCAT CATGCAGGCC CGCAAGCAGG CGAACGTACA GCCCATTGCA 
GGAGCAACTT CAGATGCAGA AGCCGGCGGC GTGTCCAATA GCGGCTCACA CGGTGGTGGC 
GGTGGCCATG AGGAGGAAGA GGAGCTCTCT GAGATCTTCA TTCACCAGAG CATCCACACC 
ATCGAGTACG TCCTAGGTTC GGTATCCCAC ACCGCTTCCT ATCTGCGATT GTGGGCGCTT 
TCCTTGGCCC ATGCCCAGTT GGCTGAGGTG CTGTGGACCA TGGTCCTATC GATTGGCTTG 
AAGCAGGAAG GGCCGGTGGG TGGCATTGTA TTGACCTGCG TGTTTGCCTT CTGGGCCATT 
CTCACCGTTG GCATTCTGGT ACTCATGGAG GGCTTGTCTG CCTTCCTGCA CACCCTGCGT 
CTCCATTGGG TCGAGTTCCA GAGCAAGTTC TACAAGGGCC AGGGTTACGC CTTCCAACCC 
TTCTCGTTTG ATGCCATCAT AGAAAACGGA GCTGCCGCCG CCGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_170396.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733275.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant D (Vha100-1), mRNA.

Price & Availability↑

CloneID	OFa06464
Clone ID Related Accession (Same CDS sequence)	NM_170395.3 , NM_170397.3 , NM_170396.2
Accession Version	NM_170396.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2511bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_170396.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGCTTCGT GGCTGGCGTC 
ATCCTGAGGG AGCGACTGCC GGCCTTCGAG CGGATGTTGT GGCGCGCCTG CAGGGGTAAC 
GTCTTCCTGC GACAGGCGAT GATTGAGACG CCGCTGGAGG ATCCCACCAA TGGCGACCAG 
GTGCACAAGT CCGTGTTTAT AATCTTCTTC CAGGGTGACC AGCTGAAGAC GCGCGTCAAG 
AAGATCTGCG AGGGCTTCCG GGCCACGCTC TATCCTTGCC CTGAAGCTCC AGCCGATCGG 
CGAGAGATGG CCATGGGTGT GATGACTCGC ATCGAAGATC TGAACACCGT GCTCGGCCAG 
ACGCAGGACC ATCGCCATCG TGTGCTGGTC GCTGCGGCCA AGAATCTTAA GAACTGGTTC 
GTCAAGGTGC GCAAGATAAA GGCCATCTAC CATACGCTGA ATCTCTTCAA TCTGGACGTG 
ACCCAGAAGT GTCTGATCGC CGAGTGCTGG GTACCGCTGC TGGACATTGA AACCATCCAG 
CTGGCCTTGC GCCGCGGAAC CGAAAGATCG GGATCGTCGG TGCCGCCAAT TCTCAACCGG 
ATGCAGACGT TCGAGAATCC ACCGACCTAC AATCGAACGA ATAAGTTTAC CAAGGCCTTT 
CAGGCATTGA TCGATGCCTA TGGCGTGGCC AGCTATCGGG AGATGAATCC GGCACCCTAC 
ACGATCATCA CCTTCCCCTT CCTGTTCGCC GTGATGTTTG GAGATTTGGG CCACGGAGCT 
ATCATGGCGC TCTTTGGTCT CTGGATGATT CGAAAGGAGA AGGGACTGGC GGCTCAGAAG 
ACGGACAACG AGATCTGGAA CATATTCTTT GGCGGACGGT ACATCATCTT CCTCATGGGC 
GTCTTCTCCA TGTACACGGG TCTTATATAC AACGATATAT TCTCCAAGTC GCTGAATATC 
TTTGGATCGC ATTGGCACTT ATCCTACAAC AAGTCCACCG TGATGGAGAA CAAGTTCCTG 
CAGTTGAGCC CGAAAGGCGA CTACGAGGGC GCTCCGTATC CGTTCGGCAT GGATCCCATT 
TGGCAGGTGG CGGGAGCCAA TAAGATCATC TTCCACAACG CTTACAAGAT GAAGATCTCG 
ATCATTTTCG GTGTTATTCA CATGATCTTC GGTGTGGTGA TGAGCTGGCA CAATCATACG 
TATTTCAGGA ACAGGATCTC CCTGCTCTAC GAGTTCATTC CCCAGCTGGT CTTCCTGCTG 
CTGCTTTTCT TCTACATGGT TTTGTTGATG TTCATCAAGT GGATCAAGTT TGCCGCCACC 
AATGATAAGC CCTACTCCGA AGCCTGTGCG CCTTCAATCC TGATCACCTT TATCGACATG 
GTGCTTTTTA ACACGCCCAA GCCACCTCCG GAGAACTGTG AAACCTATAT GTTCATGGGC 
CAGCACTTTA TCCAGGTGTT ATTCGTCCTG GTTGCCGTCG GCTGCATTCC CGTAATGTTG 
CTGGCCAAGC CGCTGCTCAT CATGCAGGCC CGCAAGCAGG CGAACGTACA GCCCATTGCA 
GGAGCAACTT CAGATGCAGA AGCCGGCGGC GTGTCCAATA GCGGCTCACA CGGTGGTGGC 
GGTGGCCATG AGGAGGAAGA GGAGCTCTCT GAGATCTTCA TTCACCAGAG CATCCACACC 
ATCGAGTACG TCCTAGGTTC GGTATCCCAC ACCGCTTCCT ATCTGCGATT GTGGGCGCTT 
TCCTTGGCCC ATGCCCAGTT GGCTGAGGTG CTGTGGACCA TGGTCCTATC GATTGGCTTG 
AAGCAGGAAG GGCCGGTGGG TGGCATTGTA TTGACCTGCG TGTTTGCCTT CTGGGCCATT 
CTCACCGTTG GCATTCTGGT ACTCATGGAG GGCTTGTCTG CCTTCCTGCA CACCCTGCGT 
CTCCATTGGG TCGAGTTCCA GAGCAAGTTC TACAAGGGCC AGGGTTACGC CTTCCAACCC 
TTCTCGTTTG ATGCCATCAT AGAAAACGGA GCTGCCGCCG CCGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733275.1
CDS	205..2715
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEQEGGVNQTTESMTRALITDEARTAGASMGPVQLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_170396.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant D (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170396.2

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGCTTCGT GGCTGGCGTC 
ATCCTGAGGG AGCGACTGCC GGCCTTCGAG CGGATGTTGT GGCGCGCCTG CAGGGGTAAC 
GTCTTCCTGC GACAGGCGAT GATTGAGACG CCGCTGGAGG ATCCCACCAA TGGCGACCAG 
GTGCACAAGT CCGTGTTTAT AATCTTCTTC CAGGGTGACC AGCTGAAGAC GCGCGTCAAG 
AAGATCTGCG AGGGCTTCCG GGCCACGCTC TATCCTTGCC CTGAAGCTCC AGCCGATCGG 
CGAGAGATGG CCATGGGTGT GATGACTCGC ATCGAAGATC TGAACACCGT GCTCGGCCAG 
ACGCAGGACC ATCGCCATCG TGTGCTGGTC GCTGCGGCCA AGAATCTTAA GAACTGGTTC 
GTCAAGGTGC GCAAGATAAA GGCCATCTAC CATACGCTGA ATCTCTTCAA TCTGGACGTG 
ACCCAGAAGT GTCTGATCGC CGAGTGCTGG GTACCGCTGC TGGACATTGA AACCATCCAG 
CTGGCCTTGC GCCGCGGAAC CGAAAGATCG GGATCGTCGG TGCCGCCAAT TCTCAACCGG 
ATGCAGACGT TCGAGAATCC ACCGACCTAC AATCGAACGA ATAAGTTTAC CAAGGCCTTT 
CAGGCATTGA TCGATGCCTA TGGCGTGGCC AGCTATCGGG AGATGAATCC GGCACCCTAC 
ACGATCATCA CCTTCCCCTT CCTGTTCGCC GTGATGTTTG GAGATTTGGG CCACGGAGCT 
ATCATGGCGC TCTTTGGTCT CTGGATGATT CGAAAGGAGA AGGGACTGGC GGCTCAGAAG 
ACGGACAACG AGATCTGGAA CATATTCTTT GGCGGACGGT ACATCATCTT CCTCATGGGC 
GTCTTCTCCA TGTACACGGG TCTTATATAC AACGATATAT TCTCCAAGTC GCTGAATATC 
TTTGGATCGC ATTGGCACTT ATCCTACAAC AAGTCCACCG TGATGGAGAA CAAGTTCCTG 
CAGTTGAGCC CGAAAGGCGA CTACGAGGGC GCTCCGTATC CGTTCGGCAT GGATCCCATT 
TGGCAGGTGG CGGGAGCCAA TAAGATCATC TTCCACAACG CTTACAAGAT GAAGATCTCG 
ATCATTTTCG GTGTTATTCA CATGATCTTC GGTGTGGTGA TGAGCTGGCA CAATCATACG 
TATTTCAGGA ACAGGATCTC CCTGCTCTAC GAGTTCATTC CCCAGCTGGT CTTCCTGCTG 
CTGCTTTTCT TCTACATGGT TTTGTTGATG TTCATCAAGT GGATCAAGTT TGCCGCCACC 
AATGATAAGC CCTACTCCGA AGCCTGTGCG CCTTCAATCC TGATCACCTT TATCGACATG 
GTGCTTTTTA ACACGCCCAA GCCACCTCCG GAGAACTGTG AAACCTATAT GTTCATGGGC 
CAGCACTTTA TCCAGGTGTT ATTCGTCCTG GTTGCCGTCG GCTGCATTCC CGTAATGTTG 
CTGGCCAAGC CGCTGCTCAT CATGCAGGCC CGCAAGCAGG CGAACGTACA GCCCATTGCA 
GGAGCAACTT CAGATGCAGA AGCCGGCGGC GTGTCCAATA GCGGCTCACA CGGTGGTGGC 
GGTGGCCATG AGGAGGAAGA GGAGCTCTCT GAGATCTTCA TTCACCAGAG CATCCACACC 
ATCGAGTACG TCCTAGGTTC GGTATCCCAC ACCGCTTCCT ATCTGCGATT GTGGGCGCTT 
TCCTTGGCCC ATGCCCAGTT GGCTGAGGTG CTGTGGACCA TGGTCCTATC GATTGGCTTG 
AAGCAGGAAG GGCCGGTGGG TGGCATTGTA TTGACCTGCG TGTTTGCCTT CTGGGCCATT 
CTCACCGTTG GCATTCTGGT ACTCATGGAG GGCTTGTCTG CCTTCCTGCA CACCCTGCGT 
CTCCATTGGG TCGAGTTCCA GAGCAAGTTC TACAAGGGCC AGGGTTACGC CTTCCAACCC 
TTCTCGTTTG ATGCCATCAT AGAAAACGGA GCTGCCGCCG CCGAGGAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_170391.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733270.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant C (Vha100-1), mRNA.

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CloneID	OFa06465
Clone ID Related Accession (Same CDS sequence)	NM_170391.3 , NM_143415.3
Accession Version	NM_170391.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform C
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170391.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGTCGGTGC CCACGGTGTA CAAGTCGAGT GGCGCATACT CATCCAGCAA ATATCGGCGC 
TATCCGCAGA TGGCCGACAA CCAAAACGAG GACGAGCAGG CGCAGCTGCT GGGCGAGGAG 
GGTGTCCGGG CCAGCCAACC GGGCCAGAAT TTGAAGCTTG GCTTCGTGGC TGGCGTCATC 
CTGAGGGAGC GACTGCCGGC CTTCGAGCGG ATGTTGTGGC GCGCCTGCAG GGGTAACGTC 
TTCCTGCGAC AGGCGATGAT TGAGACGCCG CTGGAGGATC CCACCAATGG CGACCAGGTG 
CACAAGTCCG TGTTTATAAT CTTCTTCCAG GGTGACCAGC TGAAGACGCG CGTCAAGAAG 
ATCTGCGAGG GCTTCCGGGC CACGCTCTAT CCTTGCCCTG AAGCTCCAGC CGATCGGCGA 
GAGATGGCCA TGGGTGTGAT GACTCGCATC GAAGATCTGA ACACCGTGCT CGGCCAGACG 
CAGGACCATC GCCATCGTGT GCTGGTCGCT GCGGCCAAGA ATCTTAAGAA CTGGTTCGTC 
AAGGTGCGCA AGATAAAGGC CATCTACCAT ACGCTGAATC TCTTCAATCT GGACGTGACC 
CAGAAGTGTC TGATCGCCGA GTGCTGGGTA CCGCTGCTGG ACATTGAAAC CATCCAGCTG 
GCCTTGCGCC GCGGAACCGA AAGATCGGGA TCGTCGGTGC CGCCAATTCT CAACCGGATG 
CAGACGTTCG AGAATCCACC GACCTACAAT CGAACGAATA AGTTTACCAA GGCCTTTCAG 
GCATTGATCG ATGCCTATGG CGTGGCCAGC TATCGGGAGA TGAATCCGGC ACCCTACACG 
ATCATCACCT TCCCCTTCCT GTTCGCCGTG ATGTTTGGAG ATTTGGGCCA CGGAGCTATC 
ATGGCGCTCT TTGGTCTCTG GATGATTCGA AAGGAGAAGG GACTGGCGGC TCAGAAGACG 
GACAACGAGA TCTGGAACAT ATTCTTTGGC GGACGGTACA TCATCTTCCT CATGGGCGTC 
TTCTCCATGT ACACGGGTCT TATATACAAC GATATATTCT CCAAGTCGCT GAATATCTTT 
GGATCGCATT GGCACTTATC CTACAACAAG TCCACCGTGA TGGAGAACAA GTTCCTGCAG 
TTGAGCCCGA AAGGCGACTA CGAGGGCGCT CCGTATCCGT TCGGCATGGA TCCCATTTGG 
CAGGTGGCGG GAGCCAATAA GATCATCTTC CACAACGCTT ACAAGATGAA GATCTCGATC 
ATTTTCGGTG TTATTCACAT GATCTTCGGT GTGGTGATGA GCTGGCACAA TCATACGTAT 
TTCAGGAACA GGATCTCCCT GCTCTACGAG TTCATTCCCC AGCTGGTCTT CCTGCTGCTG 
CTTTTCTTCT ACATGGTTTT GTTGATGTTC ATCAAGTGGA TCAAGTTTGC CGCCACCAAT 
GATAAGCCCT ACTCCGAAGC CTGTGCGCCT TCAATCCTGA TCACCTTTAT CGACATGGTG 
CTTTTTAACA CGCCCAAGCC ACCTCCGGAG AACTGTGAAA CCTATATGTT CATGGGCCAG 
CACTTTATCC AGGTGTTATT CGTCCTGGTT GCCGTCGGCT GCATTCCCGT AATGTTGCTG 
GCCAAGCCGC TGCTCATCAT GCAGGCCCGC AAGCAGGCGA ACGTACAGCC CATTGCAGGA 
GCAACTTCAG ATGCAGAAGC CGGCGGCGTG TCCAATAGCG GCTCACACGG TGGTGGCGGT 
GGCCATGAGG AGGAAGAGGA GCTCTCTGAG ATCTTCATTC ACCAGAGCAT CCACACCATC 
GAGTACGTCC TAGGTTCGGT ATCCCACACC GCTTCCTATC TGCGATTGTG GGCGCTTTCC 
TTGGCCCATG CCCAGTTGGC TGAGGTGCTG TGGACCATGG TCCTATCGAT TGGCTTGAAG 
CAGGAAGGGC CGGTGGGTGG CATTGTATTG ACCTGCGTGT TTGCCTTCTG GGCCATTCTC 
ACCGTTGGCA TTCTGGTACT CATGGAGGGC TTGTCTGCCT TCCTGCACAC CCTGCGTCTC 
CATTGGGTCG AGTTCCAGAG CAAGTTCTAC AAGGGCCAGG GTTACGCCTT CCAACCCTTC 
TCGTTTGATG CCATCATAGA AAACGGAGCT GCCGCCGCCG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733270.1
CDS	103..2670
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDESVPTVYKSSGAYSSSKYRRYPQMADNQNEDEQAQLLGEEGVRASQPGQNLKLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_170391.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant C (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170391.3

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGTCGGTGC CCACGGTGTA CAAGTCGAGT GGCGCATACT CATCCAGCAA ATATCGGCGC 
TATCCGCAGA TGGCCGACAA CCAAAACGAG GACGAGCAGG CGCAGCTGCT GGGCGAGGAG 
GGTGTCCGGG CCAGCCAACC GGGCCAGAAT TTGAAGCTTG GCTTCGTGGC TGGCGTCATC 
CTGAGGGAGC GACTGCCGGC CTTCGAGCGG ATGTTGTGGC GCGCCTGCAG GGGTAACGTC 
TTCCTGCGAC AGGCGATGAT TGAGACGCCG CTGGAGGATC CCACCAATGG CGACCAGGTG 
CACAAGTCCG TGTTTATAAT CTTCTTCCAG GGTGACCAGC TGAAGACGCG CGTCAAGAAG 
ATCTGCGAGG GCTTCCGGGC CACGCTCTAT CCTTGCCCTG AAGCTCCAGC CGATCGGCGA 
GAGATGGCCA TGGGTGTGAT GACTCGCATC GAAGATCTGA ACACCGTGCT CGGCCAGACG 
CAGGACCATC GCCATCGTGT GCTGGTCGCT GCGGCCAAGA ATCTTAAGAA CTGGTTCGTC 
AAGGTGCGCA AGATAAAGGC CATCTACCAT ACGCTGAATC TCTTCAATCT GGACGTGACC 
CAGAAGTGTC TGATCGCCGA GTGCTGGGTA CCGCTGCTGG ACATTGAAAC CATCCAGCTG 
GCCTTGCGCC GCGGAACCGA AAGATCGGGA TCGTCGGTGC CGCCAATTCT CAACCGGATG 
CAGACGTTCG AGAATCCACC GACCTACAAT CGAACGAATA AGTTTACCAA GGCCTTTCAG 
GCATTGATCG ATGCCTATGG CGTGGCCAGC TATCGGGAGA TGAATCCGGC ACCCTACACG 
ATCATCACCT TCCCCTTCCT GTTCGCCGTG ATGTTTGGAG ATTTGGGCCA CGGAGCTATC 
ATGGCGCTCT TTGGTCTCTG GATGATTCGA AAGGAGAAGG GACTGGCGGC TCAGAAGACG 
GACAACGAGA TCTGGAACAT ATTCTTTGGC GGACGGTACA TCATCTTCCT CATGGGCGTC 
TTCTCCATGT ACACGGGTCT TATATACAAC GATATATTCT CCAAGTCGCT GAATATCTTT 
GGATCGCATT GGCACTTATC CTACAACAAG TCCACCGTGA TGGAGAACAA GTTCCTGCAG 
TTGAGCCCGA AAGGCGACTA CGAGGGCGCT CCGTATCCGT TCGGCATGGA TCCCATTTGG 
CAGGTGGCGG GAGCCAATAA GATCATCTTC CACAACGCTT ACAAGATGAA GATCTCGATC 
ATTTTCGGTG TTATTCACAT GATCTTCGGT GTGGTGATGA GCTGGCACAA TCATACGTAT 
TTCAGGAACA GGATCTCCCT GCTCTACGAG TTCATTCCCC AGCTGGTCTT CCTGCTGCTG 
CTTTTCTTCT ACATGGTTTT GTTGATGTTC ATCAAGTGGA TCAAGTTTGC CGCCACCAAT 
GATAAGCCCT ACTCCGAAGC CTGTGCGCCT TCAATCCTGA TCACCTTTAT CGACATGGTG 
CTTTTTAACA CGCCCAAGCC ACCTCCGGAG AACTGTGAAA CCTATATGTT CATGGGCCAG 
CACTTTATCC AGGTGTTATT CGTCCTGGTT GCCGTCGGCT GCATTCCCGT AATGTTGCTG 
GCCAAGCCGC TGCTCATCAT GCAGGCCCGC AAGCAGGCGA ACGTACAGCC CATTGCAGGA 
GCAACTTCAG ATGCAGAAGC CGGCGGCGTG TCCAATAGCG GCTCACACGG TGGTGGCGGT 
GGCCATGAGG AGGAAGAGGA GCTCTCTGAG ATCTTCATTC ACCAGAGCAT CCACACCATC 
GAGTACGTCC TAGGTTCGGT ATCCCACACC GCTTCCTATC TGCGATTGTG GGCGCTTTCC 
TTGGCCCATG CCCAGTTGGC TGAGGTGCTG TGGACCATGG TCCTATCGAT TGGCTTGAAG 
CAGGAAGGGC CGGTGGGTGG CATTGTATTG ACCTGCGTGT TTGCCTTCTG GGCCATTCTC 
ACCGTTGGCA TTCTGGTACT CATGGAGGGC TTGTCTGCCT TCCTGCACAC CCTGCGTCTC 
CATTGGGTCG AGTTCCAGAG CAAGTTCTAC AAGGGCCAGG GTTACGCCTT CCAACCCTTC 
TCGTTTGATG CCATCATAGA AAACGGAGCT GCCGCCGCCG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_143415.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_651672.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant E (Vha100-1), mRNA.

Price & Availability↑

CloneID	OFa06465
Clone ID Related Accession (Same CDS sequence)	NM_170391.3 , NM_143415.3
Accession Version	NM_143415.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2568bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform E
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Dec 17, 2009 this sequence version replaced NM_143415.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGTCGGTGC CCACGGTGTA CAAGTCGAGT GGCGCATACT CATCCAGCAA ATATCGGCGC 
TATCCGCAGA TGGCCGACAA CCAAAACGAG GACGAGCAGG CGCAGCTGCT GGGCGAGGAG 
GGTGTCCGGG CCAGCCAACC GGGCCAGAAT TTGAAGCTTG GCTTCGTGGC TGGCGTCATC 
CTGAGGGAGC GACTGCCGGC CTTCGAGCGG ATGTTGTGGC GCGCCTGCAG GGGTAACGTC 
TTCCTGCGAC AGGCGATGAT TGAGACGCCG CTGGAGGATC CCACCAATGG CGACCAGGTG 
CACAAGTCCG TGTTTATAAT CTTCTTCCAG GGTGACCAGC TGAAGACGCG CGTCAAGAAG 
ATCTGCGAGG GCTTCCGGGC CACGCTCTAT CCTTGCCCTG AAGCTCCAGC CGATCGGCGA 
GAGATGGCCA TGGGTGTGAT GACTCGCATC GAAGATCTGA ACACCGTGCT CGGCCAGACG 
CAGGACCATC GCCATCGTGT GCTGGTCGCT GCGGCCAAGA ATCTTAAGAA CTGGTTCGTC 
AAGGTGCGCA AGATAAAGGC CATCTACCAT ACGCTGAATC TCTTCAATCT GGACGTGACC 
CAGAAGTGTC TGATCGCCGA GTGCTGGGTA CCGCTGCTGG ACATTGAAAC CATCCAGCTG 
GCCTTGCGCC GCGGAACCGA AAGATCGGGA TCGTCGGTGC CGCCAATTCT CAACCGGATG 
CAGACGTTCG AGAATCCACC GACCTACAAT CGAACGAATA AGTTTACCAA GGCCTTTCAG 
GCATTGATCG ATGCCTATGG CGTGGCCAGC TATCGGGAGA TGAATCCGGC ACCCTACACG 
ATCATCACCT TCCCCTTCCT GTTCGCCGTG ATGTTTGGAG ATTTGGGCCA CGGAGCTATC 
ATGGCGCTCT TTGGTCTCTG GATGATTCGA AAGGAGAAGG GACTGGCGGC TCAGAAGACG 
GACAACGAGA TCTGGAACAT ATTCTTTGGC GGACGGTACA TCATCTTCCT CATGGGCGTC 
TTCTCCATGT ACACGGGTCT TATATACAAC GATATATTCT CCAAGTCGCT GAATATCTTT 
GGATCGCATT GGCACTTATC CTACAACAAG TCCACCGTGA TGGAGAACAA GTTCCTGCAG 
TTGAGCCCGA AAGGCGACTA CGAGGGCGCT CCGTATCCGT TCGGCATGGA TCCCATTTGG 
CAGGTGGCGG GAGCCAATAA GATCATCTTC CACAACGCTT ACAAGATGAA GATCTCGATC 
ATTTTCGGTG TTATTCACAT GATCTTCGGT GTGGTGATGA GCTGGCACAA TCATACGTAT 
TTCAGGAACA GGATCTCCCT GCTCTACGAG TTCATTCCCC AGCTGGTCTT CCTGCTGCTG 
CTTTTCTTCT ACATGGTTTT GTTGATGTTC ATCAAGTGGA TCAAGTTTGC CGCCACCAAT 
GATAAGCCCT ACTCCGAAGC CTGTGCGCCT TCAATCCTGA TCACCTTTAT CGACATGGTG 
CTTTTTAACA CGCCCAAGCC ACCTCCGGAG AACTGTGAAA CCTATATGTT CATGGGCCAG 
CACTTTATCC AGGTGTTATT CGTCCTGGTT GCCGTCGGCT GCATTCCCGT AATGTTGCTG 
GCCAAGCCGC TGCTCATCAT GCAGGCCCGC AAGCAGGCGA ACGTACAGCC CATTGCAGGA 
GCAACTTCAG ATGCAGAAGC CGGCGGCGTG TCCAATAGCG GCTCACACGG TGGTGGCGGT 
GGCCATGAGG AGGAAGAGGA GCTCTCTGAG ATCTTCATTC ACCAGAGCAT CCACACCATC 
GAGTACGTCC TAGGTTCGGT ATCCCACACC GCTTCCTATC TGCGATTGTG GGCGCTTTCC 
TTGGCCCATG CCCAGTTGGC TGAGGTGCTG TGGACCATGG TCCTATCGAT TGGCTTGAAG 
CAGGAAGGGC CGGTGGGTGG CATTGTATTG ACCTGCGTGT TTGCCTTCTG GGCCATTCTC 
ACCGTTGGCA TTCTGGTACT CATGGAGGGC TTGTCTGCCT TCCTGCACAC CCTGCGTCTC 
CATTGGGTCG AGTTCCAGAG CAAGTTCTAC AAGGGCCAGG GTTACGCCTT CCAACCCTTC 
TCGTTTGATG CCATCATAGA AAACGGAGCT GCCGCCGCCG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_651672.1
CDS	205..2772
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDESVPTVYKSSGAYSSSKYRRYPQMADNQNEDEQAQLLGEEGVRASQPGQNLKLGFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_143415.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant E (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_143415.3

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGTCGGTGC CCACGGTGTA CAAGTCGAGT GGCGCATACT CATCCAGCAA ATATCGGCGC 
TATCCGCAGA TGGCCGACAA CCAAAACGAG GACGAGCAGG CGCAGCTGCT GGGCGAGGAG 
GGTGTCCGGG CCAGCCAACC GGGCCAGAAT TTGAAGCTTG GCTTCGTGGC TGGCGTCATC 
CTGAGGGAGC GACTGCCGGC CTTCGAGCGG ATGTTGTGGC GCGCCTGCAG GGGTAACGTC 
TTCCTGCGAC AGGCGATGAT TGAGACGCCG CTGGAGGATC CCACCAATGG CGACCAGGTG 
CACAAGTCCG TGTTTATAAT CTTCTTCCAG GGTGACCAGC TGAAGACGCG CGTCAAGAAG 
ATCTGCGAGG GCTTCCGGGC CACGCTCTAT CCTTGCCCTG AAGCTCCAGC CGATCGGCGA 
GAGATGGCCA TGGGTGTGAT GACTCGCATC GAAGATCTGA ACACCGTGCT CGGCCAGACG 
CAGGACCATC GCCATCGTGT GCTGGTCGCT GCGGCCAAGA ATCTTAAGAA CTGGTTCGTC 
AAGGTGCGCA AGATAAAGGC CATCTACCAT ACGCTGAATC TCTTCAATCT GGACGTGACC 
CAGAAGTGTC TGATCGCCGA GTGCTGGGTA CCGCTGCTGG ACATTGAAAC CATCCAGCTG 
GCCTTGCGCC GCGGAACCGA AAGATCGGGA TCGTCGGTGC CGCCAATTCT CAACCGGATG 
CAGACGTTCG AGAATCCACC GACCTACAAT CGAACGAATA AGTTTACCAA GGCCTTTCAG 
GCATTGATCG ATGCCTATGG CGTGGCCAGC TATCGGGAGA TGAATCCGGC ACCCTACACG 
ATCATCACCT TCCCCTTCCT GTTCGCCGTG ATGTTTGGAG ATTTGGGCCA CGGAGCTATC 
ATGGCGCTCT TTGGTCTCTG GATGATTCGA AAGGAGAAGG GACTGGCGGC TCAGAAGACG 
GACAACGAGA TCTGGAACAT ATTCTTTGGC GGACGGTACA TCATCTTCCT CATGGGCGTC 
TTCTCCATGT ACACGGGTCT TATATACAAC GATATATTCT CCAAGTCGCT GAATATCTTT 
GGATCGCATT GGCACTTATC CTACAACAAG TCCACCGTGA TGGAGAACAA GTTCCTGCAG 
TTGAGCCCGA AAGGCGACTA CGAGGGCGCT CCGTATCCGT TCGGCATGGA TCCCATTTGG 
CAGGTGGCGG GAGCCAATAA GATCATCTTC CACAACGCTT ACAAGATGAA GATCTCGATC 
ATTTTCGGTG TTATTCACAT GATCTTCGGT GTGGTGATGA GCTGGCACAA TCATACGTAT 
TTCAGGAACA GGATCTCCCT GCTCTACGAG TTCATTCCCC AGCTGGTCTT CCTGCTGCTG 
CTTTTCTTCT ACATGGTTTT GTTGATGTTC ATCAAGTGGA TCAAGTTTGC CGCCACCAAT 
GATAAGCCCT ACTCCGAAGC CTGTGCGCCT TCAATCCTGA TCACCTTTAT CGACATGGTG 
CTTTTTAACA CGCCCAAGCC ACCTCCGGAG AACTGTGAAA CCTATATGTT CATGGGCCAG 
CACTTTATCC AGGTGTTATT CGTCCTGGTT GCCGTCGGCT GCATTCCCGT AATGTTGCTG 
GCCAAGCCGC TGCTCATCAT GCAGGCCCGC AAGCAGGCGA ACGTACAGCC CATTGCAGGA 
GCAACTTCAG ATGCAGAAGC CGGCGGCGTG TCCAATAGCG GCTCACACGG TGGTGGCGGT 
GGCCATGAGG AGGAAGAGGA GCTCTCTGAG ATCTTCATTC ACCAGAGCAT CCACACCATC 
GAGTACGTCC TAGGTTCGGT ATCCCACACC GCTTCCTATC TGCGATTGTG GGCGCTTTCC 
TTGGCCCATG CCCAGTTGGC TGAGGTGCTG TGGACCATGG TCCTATCGAT TGGCTTGAAG 
CAGGAAGGGC CGGTGGGTGG CATTGTATTG ACCTGCGTGT TTGCCTTCTG GGCCATTCTC 
ACCGTTGGCA TTCTGGTACT CATGGAGGGC TTGTCTGCCT TCCTGCACAC CCTGCGTCTC 
CATTGGGTCG AGTTCCAGAG CAAGTTCTAC AAGGGCCAGG GTTACGCCTT CCAACCCTTC 
TCGTTTGATG CCATCATAGA AAACGGAGCT GCCGCCGCCG AGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_170394.3 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_733273.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant G (Vha100-1), mRNA.

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CloneID	OFa06466
Clone ID Related Accession (Same CDS sequence)	NM_170394.3
Accession Version	NM_170394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2553bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform G
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_170394.2. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGTTACAT GGAGAAATCA 
AACGAACGTG AGGATTATCT TCCATGCTTC GTGGCTGGCG TCATCCTGAG GGAGCGACTG 
CCGGCCTTCG AGCGGATGTT GTGGCGCGCC TGCAGGGGTA ACGTCTTCCT GCGACAGGCG 
ATGATTGAGA CGCCGCTGGA GGATCCCACC AATGGCGACC AGGTGCACAA GTCCGTGTTT 
ATAATCTTCT TCCAGGGTGA CCAGCTGAAG ACGCGCGTCA AGAAGATCTG CGAGGGCTTC 
CGGGCCACGC TCTATCCTTG CCCTGAAGCT CCAGCCGATC GGCGAGAGAT GGCCATGGGT 
GTGATGACTC GCATCGAAGA TCTGAACACC GTGCTCGGCC AGACGCAGGA CCATCGCCAT 
CGTGTGCTGG TCGCTGCGGC CAAGAATCTT AAGAACTGGT TCGTCAAGGT GCGCAAGATA 
AAGGCCATCT ACCATACGCT GAATCTCTTC AATCTGGACG TGACCCAGAA GTGTCTGATC 
GCCGAGTGCT GGGTACCGCT GCTGGACATT GAAACCATCC AGCTGGCCTT GCGCCGCGGA 
ACCGAAAGAT CGGGATCGTC GGTGCCGCCA ATTCTCAACC GGATGCAGAC GTTCGAGAAT 
CCACCGACCT ACAATCGAAC GAATAAGTTT ACCAAGGCCT TTCAGGCATT GATCGATGCC 
TATGGCGTGG CCAGCTATCG GGAGATGAAT CCGGCACCCT ACACGATCAT CACCTTCCCC 
TTCCTGTTCG CCGTGATGTT TGGAGATTTG GGCCACGGAG CTATCATGGC GCTCTTTGGT 
CTCTGGATGA TTCGAAAGGA GAAGGGACTG GCGGCTCAGA AGACGGACAA CGAGATCTGG 
AACATATTCT TTGGCGGACG GTACATCATC TTCCTCATGG GCGTCTTCTC CATGTACACG 
GGTCTTATAT ACAACGATAT ATTCTCCAAG TCGCTGAATA TCTTTGGATC GCATTGGCAC 
TTATCCTACA ACAAGTCCAC CGTGATGGAG AACAAGTTCC TGCAGTTGAG CCCGAAAGGC 
GACTACGAGG GCGCTCCGTA TCCGTTCGGC ATGGATCCCA TTTGGCAGGT GGCGGGAGCC 
AATAAGATCA TCTTCCACAA CGCTTACAAG ATGAAGATCT CGATCATTTT CGGTGTTATT 
CACATGATCT TCGGTGTGGT GATGAGCTGG CACAATCATA CGTATTTCAG GAACAGGATC 
TCCCTGCTCT ACGAGTTCAT TCCCCAGCTG GTCTTCCTGC TGCTGCTTTT CTTCTACATG 
GTTTTGTTGA TGTTCATCAA GTGGATCAAG TTTGCCGCCA CCAATGATAA GCCCTACTCC 
GAAGCCTGTG CGCCTTCAAT CCTGATCACC TTTATCGACA TGGTGCTTTT TAACACGCCC 
AAGCCACCTC CGGAGAACTG TGAAACCTAT ATGTTCATGG GCCAGCACTT TATCCAGGTG 
TTATTCGTCC TGGTTGCCGT CGGCTGCATT CCCGTAATGT TGCTGGCCAA GCCGCTGCTC 
ATCATGCAGG CCCGCAAGCA GGCGAACGTA CAGCCCATTG CAGGAGCAAC TTCAGATGCA 
GAAGCCGGCG GCGTGTCCAA TAGCGGCTCA CACGGTGGTG GCGGTGGCCA TGAGGAGGAA 
GAGGAGCTCT CTGAGATCTT CATTCACCAG AGCATCCACA CCATCGAGTA CGTCCTAGGT 
TCGGTATCCC ACACCGCTTC CTATCTGCGA TTGTGGGCGC TTTCCTTGGC CCATGCCCAG 
TTGGCTGAGG TGCTGTGGAC CATGGTCCTA TCGATTGGCT TGAAGCAGGA AGGGCCGGTG 
GGTGGCATTG TATTGACCTG CGTGTTTGCC TTCTGGGCCA TTCTCACCGT TGGCATTCTG 
GTACTCATGG AGGGCTTGTC TGCCTTCCTG CACACCCTGC GTCTCCATTG GGTCGAGTTC 
CAGAGCAAGT TCTACAAGGG CCAGGGTTAC GCCTTCCAAC CCTTCTCGTT TGATGCCATC 
ATAGAAAACG GAGCTGCCGC CGCCGAGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_733273.1
CDS	103..2655
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEQEGGVNQTTESMTRALITDEARTAGASMGPVQLGYMEKSNEREDYLPCFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_170394.3
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant G (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_170394.3

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGTTACAT GGAGAAATCA 
AACGAACGTG AGGATTATCT TCCATGCTTC GTGGCTGGCG TCATCCTGAG GGAGCGACTG 
CCGGCCTTCG AGCGGATGTT GTGGCGCGCC TGCAGGGGTA ACGTCTTCCT GCGACAGGCG 
ATGATTGAGA CGCCGCTGGA GGATCCCACC AATGGCGACC AGGTGCACAA GTCCGTGTTT 
ATAATCTTCT TCCAGGGTGA CCAGCTGAAG ACGCGCGTCA AGAAGATCTG CGAGGGCTTC 
CGGGCCACGC TCTATCCTTG CCCTGAAGCT CCAGCCGATC GGCGAGAGAT GGCCATGGGT 
GTGATGACTC GCATCGAAGA TCTGAACACC GTGCTCGGCC AGACGCAGGA CCATCGCCAT 
CGTGTGCTGG TCGCTGCGGC CAAGAATCTT AAGAACTGGT TCGTCAAGGT GCGCAAGATA 
AAGGCCATCT ACCATACGCT GAATCTCTTC AATCTGGACG TGACCCAGAA GTGTCTGATC 
GCCGAGTGCT GGGTACCGCT GCTGGACATT GAAACCATCC AGCTGGCCTT GCGCCGCGGA 
ACCGAAAGAT CGGGATCGTC GGTGCCGCCA ATTCTCAACC GGATGCAGAC GTTCGAGAAT 
CCACCGACCT ACAATCGAAC GAATAAGTTT ACCAAGGCCT TTCAGGCATT GATCGATGCC 
TATGGCGTGG CCAGCTATCG GGAGATGAAT CCGGCACCCT ACACGATCAT CACCTTCCCC 
TTCCTGTTCG CCGTGATGTT TGGAGATTTG GGCCACGGAG CTATCATGGC GCTCTTTGGT 
CTCTGGATGA TTCGAAAGGA GAAGGGACTG GCGGCTCAGA AGACGGACAA CGAGATCTGG 
AACATATTCT TTGGCGGACG GTACATCATC TTCCTCATGG GCGTCTTCTC CATGTACACG 
GGTCTTATAT ACAACGATAT ATTCTCCAAG TCGCTGAATA TCTTTGGATC GCATTGGCAC 
TTATCCTACA ACAAGTCCAC CGTGATGGAG AACAAGTTCC TGCAGTTGAG CCCGAAAGGC 
GACTACGAGG GCGCTCCGTA TCCGTTCGGC ATGGATCCCA TTTGGCAGGT GGCGGGAGCC 
AATAAGATCA TCTTCCACAA CGCTTACAAG ATGAAGATCT CGATCATTTT CGGTGTTATT 
CACATGATCT TCGGTGTGGT GATGAGCTGG CACAATCATA CGTATTTCAG GAACAGGATC 
TCCCTGCTCT ACGAGTTCAT TCCCCAGCTG GTCTTCCTGC TGCTGCTTTT CTTCTACATG 
GTTTTGTTGA TGTTCATCAA GTGGATCAAG TTTGCCGCCA CCAATGATAA GCCCTACTCC 
GAAGCCTGTG CGCCTTCAAT CCTGATCACC TTTATCGACA TGGTGCTTTT TAACACGCCC 
AAGCCACCTC CGGAGAACTG TGAAACCTAT ATGTTCATGG GCCAGCACTT TATCCAGGTG 
TTATTCGTCC TGGTTGCCGT CGGCTGCATT CCCGTAATGT TGCTGGCCAA GCCGCTGCTC 
ATCATGCAGG CCCGCAAGCA GGCGAACGTA CAGCCCATTG CAGGAGCAAC TTCAGATGCA 
GAAGCCGGCG GCGTGTCCAA TAGCGGCTCA CACGGTGGTG GCGGTGGCCA TGAGGAGGAA 
GAGGAGCTCT CTGAGATCTT CATTCACCAG AGCATCCACA CCATCGAGTA CGTCCTAGGT 
TCGGTATCCC ACACCGCTTC CTATCTGCGA TTGTGGGCGC TTTCCTTGGC CCATGCCCAG 
TTGGCTGAGG TGCTGTGGAC CATGGTCCTA TCGATTGGCT TGAAGCAGGA AGGGCCGGTG 
GGTGGCATTG TATTGACCTG CGTGTTTGCC TTCTGGGCCA TTCTCACCGT TGGCATTCTG 
GTACTCATGG AGGGCTTGTC TGCCTTCCTG CACACCCTGC GTCTCCATTG GGTCGAGTTC 
CAGAGCAAGT TCTACAAGGG CCAGGGTTAC GCCTTCCAAC CCTTCTCGTT TGATGCCATC 
ATAGAAAACG GAGCTGCCGC CGCCGAGGAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_001170297.1 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163768.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant I (Vha100-1), mRNA.

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CloneID	OFa08942
Clone ID Related Accession (Same CDS sequence)	NM_001170297.1 , NM_001170298.2
Accession Version	NM_001170297.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform I
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGTTACAT GGAGAAATCA 
AACGAACGTG AGGATTATCT TCCATGCTTC GTGGCTGGCG TCATCCTGAG GGAGCGACTG 
CCGGCCTTCG AGCGGATGTT GTGGCGCGCC TGCAGGGGTA ACGTCTTCCT GCGACAGGCG 
ATGATTGAGA CGCCGCTGGA GGATCCCACC AATGGCGACC AGGTGCACAA GTCCGTGTTT 
ATAATCTTCT TCCAGGGTGA CCAGCTGAAG ACGCGCGTCA AGAAGATCTG CGAGGGCTTC 
CGGGCCACGC TCTATCCTTG CCCTGAAGCT CCAGCCGATC GGCGAGAGAT GGCCATGGGT 
GTGATGACTC GCATCGAAGA TCTGAACACC GTGCTCGGCC AGACGCAGGA CCATCGCCAT 
CGTGTGCTGG TCGCTGCGGC CAAGAATCTT AAGAACTGGT TCGTCAAGGT GCGCAAGATA 
AAGGCCATCT ACCATACGCT GAATCTCTTC AATCTGGACG TGACCCAGAA GTGTCTGATC 
GCCGAGTGCT GGGTACCGCT GCTGGACATT GAAACCATCC AGCTGGCCTT GCGCCGCGGA 
ACCGAAAGAT CGGGATCGTC GGTGCCGCCA ATTCTCAACC GGATGCAGAC GTTCGAGAAT 
CCACCGACCT ACAATCGAAC GAATAAGTTT ACCAAGGCCT TTCAGGCATT GATCGATGCC 
TATGGCGTGG CCAGCTATCG GGAGATGAAT CCGGCACCCT ACACGATCAT CACCTTCCCC 
TTCCTGTTCG CCGTGATGTT TGGAGATTTG GGCCACGGAG CTATCATGGC GCTCTTTGGT 
CTCTGGATGA TTCGAAAGGA GAAGGGACTG GCGGCTCAGA AGACGGACAA CGAGATCTGG 
AACATATTCT TTGGCGGACG GTACATCATC TTCCTCATGG GCGTCTTCTC CATGTACACG 
GGTCTTATAT ACAACGATAT ATTCTCCAAG TCGCTGAATA TCTTTGGATC GCATTGGCAC 
TTATCCTACA ACAAGTCCAC CGTGATGGAG AACAAGTTCC TGCAGTTGAG CCCGAAAGGC 
GACTACGAGG GCGCTCCGTA TCCGTTCGGC ATGGATCCCA TTTGGCAGGT GGCGGGAGCC 
AATAAGATCA TCTTCCACAA CGCTTACAAG ATGAAGATCT CGATCATTTT CGGTGTTATT 
CACATGATCT TCGGTGTGGT GATGAGCTGG CACAATCATA CGTATTTCAG GAACAGGATC 
TCCCTGCTCT ACGAGTTCAT TCCCCAGCTG GTCTTCCTGC TGCTGCTTTT CTTCTACATG 
GTTTTGTTGA TGTTCATCAA GTGGATCAAG TTTGCCGCCA CCAATGATAA GCCCTACTCC 
GAAGCCTGTG CGCCTTCAAT CCTGATCACC TTTATCGACA TGGTGCTTTT TAACACGCCC 
AAGCCACCTC CGGAGAACTG TGAAACCTAT ATGTTCATGG GCCAGCACTT TATCCAGGTG 
TTATTCGTCC TGGTTGCCGT CGGCTGCATT CCCGTAATGT TGCTGGCCAA GCCGCTGCTC 
ATCATGCAGG CCCGCAAGCA GGCGAACGAA GAGGTACAGC CCATTGCAGG AGCAACTTCA 
GATGCAGAAG CCGGCGGCGT GTCCAATAGC GGCTCACACG GTGGTGGCGG TGGCCATGAG 
GAGGAAGAGG AGCTCTCTGA GATCTTCATT CACCAGAGCA TCCACACCAT CGAGTACGTC 
CTAGGTTCGG TATCCCACAC CGCTTCCTAT CTGCGATTGT GGGCGCTTTC CTTGGCCCAT 
GCCCAGTTGG CTGAGGTGCT GTGGACCATG GTCCTATCGA TTGGCTTGAA GCAGGAAGGG 
CCGGTGGGTG GCATTGTATT GACCTGCGTG TTTGCCTTCT GGGCCATTCT CACCGTTGGC 
ATTCTGGTAC TCATGGAGGG CTTGTCTGCC TTCCTGCACA CCCTGCGTCT CCATTGGGTC 
GAGTTCCAGA GCAAGTTCTA CAAGGGCCAG GGTTACGCCT TCCAACCCTT CTCGTTTGAT 
GCCATCATAG AAAACGGAGC TGCCGCCGCC GAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163768.1
CDS	205..2763
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEQEGGVNQTTESMTRALITDEARTAGASMGPVQLGYMEKSNEREDYLPCFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANEEVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_001170297.1
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant I (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170297.1

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGTTACAT GGAGAAATCA 
AACGAACGTG AGGATTATCT TCCATGCTTC GTGGCTGGCG TCATCCTGAG GGAGCGACTG 
CCGGCCTTCG AGCGGATGTT GTGGCGCGCC TGCAGGGGTA ACGTCTTCCT GCGACAGGCG 
ATGATTGAGA CGCCGCTGGA GGATCCCACC AATGGCGACC AGGTGCACAA GTCCGTGTTT 
ATAATCTTCT TCCAGGGTGA CCAGCTGAAG ACGCGCGTCA AGAAGATCTG CGAGGGCTTC 
CGGGCCACGC TCTATCCTTG CCCTGAAGCT CCAGCCGATC GGCGAGAGAT GGCCATGGGT 
GTGATGACTC GCATCGAAGA TCTGAACACC GTGCTCGGCC AGACGCAGGA CCATCGCCAT 
CGTGTGCTGG TCGCTGCGGC CAAGAATCTT AAGAACTGGT TCGTCAAGGT GCGCAAGATA 
AAGGCCATCT ACCATACGCT GAATCTCTTC AATCTGGACG TGACCCAGAA GTGTCTGATC 
GCCGAGTGCT GGGTACCGCT GCTGGACATT GAAACCATCC AGCTGGCCTT GCGCCGCGGA 
ACCGAAAGAT CGGGATCGTC GGTGCCGCCA ATTCTCAACC GGATGCAGAC GTTCGAGAAT 
CCACCGACCT ACAATCGAAC GAATAAGTTT ACCAAGGCCT TTCAGGCATT GATCGATGCC 
TATGGCGTGG CCAGCTATCG GGAGATGAAT CCGGCACCCT ACACGATCAT CACCTTCCCC 
TTCCTGTTCG CCGTGATGTT TGGAGATTTG GGCCACGGAG CTATCATGGC GCTCTTTGGT 
CTCTGGATGA TTCGAAAGGA GAAGGGACTG GCGGCTCAGA AGACGGACAA CGAGATCTGG 
AACATATTCT TTGGCGGACG GTACATCATC TTCCTCATGG GCGTCTTCTC CATGTACACG 
GGTCTTATAT ACAACGATAT ATTCTCCAAG TCGCTGAATA TCTTTGGATC GCATTGGCAC 
TTATCCTACA ACAAGTCCAC CGTGATGGAG AACAAGTTCC TGCAGTTGAG CCCGAAAGGC 
GACTACGAGG GCGCTCCGTA TCCGTTCGGC ATGGATCCCA TTTGGCAGGT GGCGGGAGCC 
AATAAGATCA TCTTCCACAA CGCTTACAAG ATGAAGATCT CGATCATTTT CGGTGTTATT 
CACATGATCT TCGGTGTGGT GATGAGCTGG CACAATCATA CGTATTTCAG GAACAGGATC 
TCCCTGCTCT ACGAGTTCAT TCCCCAGCTG GTCTTCCTGC TGCTGCTTTT CTTCTACATG 
GTTTTGTTGA TGTTCATCAA GTGGATCAAG TTTGCCGCCA CCAATGATAA GCCCTACTCC 
GAAGCCTGTG CGCCTTCAAT CCTGATCACC TTTATCGACA TGGTGCTTTT TAACACGCCC 
AAGCCACCTC CGGAGAACTG TGAAACCTAT ATGTTCATGG GCCAGCACTT TATCCAGGTG 
TTATTCGTCC TGGTTGCCGT CGGCTGCATT CCCGTAATGT TGCTGGCCAA GCCGCTGCTC 
ATCATGCAGG CCCGCAAGCA GGCGAACGAA GAGGTACAGC CCATTGCAGG AGCAACTTCA 
GATGCAGAAG CCGGCGGCGT GTCCAATAGC GGCTCACACG GTGGTGGCGG TGGCCATGAG 
GAGGAAGAGG AGCTCTCTGA GATCTTCATT CACCAGAGCA TCCACACCAT CGAGTACGTC 
CTAGGTTCGG TATCCCACAC CGCTTCCTAT CTGCGATTGT GGGCGCTTTC CTTGGCCCAT 
GCCCAGTTGG CTGAGGTGCT GTGGACCATG GTCCTATCGA TTGGCTTGAA GCAGGAAGGG 
CCGGTGGGTG GCATTGTATT GACCTGCGTG TTTGCCTTCT GGGCCATTCT CACCGTTGGC 
ATTCTGGTAC TCATGGAGGG CTTGTCTGCC TTCCTGCACA CCCTGCGTCT CCATTGGGTC 
GAGTTCCAGA GCAAGTTCTA CAAGGGCCAG GGTTACGCCT TCCAACCCTT CTCGTTTGAT 
GCCATCATAG AAAACGGAGC TGCCGCCGCC GAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Vha100-1 ( NM_001170298.2 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_001163769.1 Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant J (Vha100-1), mRNA.

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CloneID	OFa08942
Clone ID Related Accession (Same CDS sequence)	NM_001170297.1 , NM_001170298.2
Accession Version	NM_001170298.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2559bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	vacuolar H[+] ATPase 100kD subunit 1, isoform J
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033777). On Jan 16, 2013 this sequence version replaced NM_001170298.1. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGTTACAT GGAGAAATCA 
AACGAACGTG AGGATTATCT TCCATGCTTC GTGGCTGGCG TCATCCTGAG GGAGCGACTG 
CCGGCCTTCG AGCGGATGTT GTGGCGCGCC TGCAGGGGTA ACGTCTTCCT GCGACAGGCG 
ATGATTGAGA CGCCGCTGGA GGATCCCACC AATGGCGACC AGGTGCACAA GTCCGTGTTT 
ATAATCTTCT TCCAGGGTGA CCAGCTGAAG ACGCGCGTCA AGAAGATCTG CGAGGGCTTC 
CGGGCCACGC TCTATCCTTG CCCTGAAGCT CCAGCCGATC GGCGAGAGAT GGCCATGGGT 
GTGATGACTC GCATCGAAGA TCTGAACACC GTGCTCGGCC AGACGCAGGA CCATCGCCAT 
CGTGTGCTGG TCGCTGCGGC CAAGAATCTT AAGAACTGGT TCGTCAAGGT GCGCAAGATA 
AAGGCCATCT ACCATACGCT GAATCTCTTC AATCTGGACG TGACCCAGAA GTGTCTGATC 
GCCGAGTGCT GGGTACCGCT GCTGGACATT GAAACCATCC AGCTGGCCTT GCGCCGCGGA 
ACCGAAAGAT CGGGATCGTC GGTGCCGCCA ATTCTCAACC GGATGCAGAC GTTCGAGAAT 
CCACCGACCT ACAATCGAAC GAATAAGTTT ACCAAGGCCT TTCAGGCATT GATCGATGCC 
TATGGCGTGG CCAGCTATCG GGAGATGAAT CCGGCACCCT ACACGATCAT CACCTTCCCC 
TTCCTGTTCG CCGTGATGTT TGGAGATTTG GGCCACGGAG CTATCATGGC GCTCTTTGGT 
CTCTGGATGA TTCGAAAGGA GAAGGGACTG GCGGCTCAGA AGACGGACAA CGAGATCTGG 
AACATATTCT TTGGCGGACG GTACATCATC TTCCTCATGG GCGTCTTCTC CATGTACACG 
GGTCTTATAT ACAACGATAT ATTCTCCAAG TCGCTGAATA TCTTTGGATC GCATTGGCAC 
TTATCCTACA ACAAGTCCAC CGTGATGGAG AACAAGTTCC TGCAGTTGAG CCCGAAAGGC 
GACTACGAGG GCGCTCCGTA TCCGTTCGGC ATGGATCCCA TTTGGCAGGT GGCGGGAGCC 
AATAAGATCA TCTTCCACAA CGCTTACAAG ATGAAGATCT CGATCATTTT CGGTGTTATT 
CACATGATCT TCGGTGTGGT GATGAGCTGG CACAATCATA CGTATTTCAG GAACAGGATC 
TCCCTGCTCT ACGAGTTCAT TCCCCAGCTG GTCTTCCTGC TGCTGCTTTT CTTCTACATG 
GTTTTGTTGA TGTTCATCAA GTGGATCAAG TTTGCCGCCA CCAATGATAA GCCCTACTCC 
GAAGCCTGTG CGCCTTCAAT CCTGATCACC TTTATCGACA TGGTGCTTTT TAACACGCCC 
AAGCCACCTC CGGAGAACTG TGAAACCTAT ATGTTCATGG GCCAGCACTT TATCCAGGTG 
TTATTCGTCC TGGTTGCCGT CGGCTGCATT CCCGTAATGT TGCTGGCCAA GCCGCTGCTC 
ATCATGCAGG CCCGCAAGCA GGCGAACGAA GAGGTACAGC CCATTGCAGG AGCAACTTCA 
GATGCAGAAG CCGGCGGCGT GTCCAATAGC GGCTCACACG GTGGTGGCGG TGGCCATGAG 
GAGGAAGAGG AGCTCTCTGA GATCTTCATT CACCAGAGCA TCCACACCAT CGAGTACGTC 
CTAGGTTCGG TATCCCACAC CGCTTCCTAT CTGCGATTGT GGGCGCTTTC CTTGGCCCAT 
GCCCAGTTGG CTGAGGTGCT GTGGACCATG GTCCTATCGA TTGGCTTGAA GCAGGAAGGG 
CCGGTGGGTG GCATTGTATT GACCTGCGTG TTTGCCTTCT GGGCCATTCT CACCGTTGGC 
ATTCTGGTAC TCATGGAGGG CTTGTCTGCC TTCCTGCACA CCCTGCGTCT CCATTGGGTC 
GAGTTCCAGA GCAAGTTCTA CAAGGGCCAG GGTTACGCCT TCCAACCCTT CTCGTTTGAT 
GCCATCATAG AAAACGGAGC TGCCGCCGCC GAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001163769.1
CDS	103..2661
Translation	MGSLFRSEEMALCQLFLQSEAAYACVSELGELGLVQFRDLNPDVNAFQRKFVNEVRRCDEMERKLRYLEKEIKKDGIPMLDTGESPEAPQPREMIDLEATFEKLENELREVNQNAEALKRNFLELTELKHILRKTQVFFDEQEGGVNQTTESMTRALITDEARTAGASMGPVQLGYMEKSNEREDYLPCFVAGVILRERLPAFERMLWRACRGNVFLRQAMIETPLEDPTNGDQVHKSVFIIFFQGDQLKTRVKKICEGFRATLYPCPEAPADRREMAMGVMTRIEDLNTVLGQTQDHRHRVLVAAAKNLKNWFVKVRKIKAIYHTLNLFNLDVTQKCLIAECWVPLLDIETIQLALRRGTERSGSSVPPILNRMQTFENPPTYNRTNKFTKAFQALIDAYGVASYREMNPAPYTIITFPFLFAVMFGDLGHGAIMALFGLWMIRKEKGLAAQKTDNEIWNIFFGGRYIIFLMGVFSMYTGLIYNDIFSKSLNIFGSHWHLSYNKSTVMENKFLQLSPKGDYEGAPYPFGMDPIWQVAGANKIIFHNAYKMKISIIFGVIHMIFGVVMSWHNHTYFRNRISLLYEFIPQLVFLLLLFFYMVLLMFIKWIKFAATNDKPYSEACAPSILITFIDMVLFNTPKPPPENCETYMFMGQHFIQVLFVLVAVGCIPVMLLAKPLLIMQARKQANEEVQPIAGATSDAEAGGVSNSGSHGGGGGHEEEEELSEIFIHQSIHTIEYVLGSVSHTASYLRLWALSLAHAQLAEVLWTMVLSIGLKQEGPVGGIVLTCVFAFWAILTVGILVLMEGLSAFLHTLRLHWVEFQSKFYKGQGYAFQPFSFDAIIENGAAAAEE

Target ORF information:

RefSeq Version	NM_001170298.2
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster vacuolar H[+] ATPase 100kD subunit 1, transcript variant J (Vha100-1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001170298.2

ORF Insert Sequence:

ATGGGTTCCC TATTCCGCAG CGAGGAAATG GCGCTGTGCC AGCTGTTCCT GCAGAGCGAG 
GCGGCCTACG CCTGCGTCTC CGAGTTGGGA GAACTGGGAT TGGTCCAGTT CCGAGATCTC 
AATCCCGATG TCAACGCTTT CCAGAGGAAG TTCGTCAACG AGGTGCGCCG CTGCGATGAG 
ATGGAGCGCA AGCTGCGTTA CCTGGAGAAG GAGATCAAGA AGGACGGCAT CCCCATGTTG 
GACACCGGGG AGAGTCCCGA GGCCCCGCAG CCCCGCGAGA TGATCGACCT GGAGGCCACC 
TTTGAGAAGC TGGAGAACGA GCTGAGGGAG GTGAATCAGA ACGCCGAGGC CCTGAAGCGC 
AACTTTCTGG AGCTGACCGA GCTGAAGCAT ATTTTGCGCA AAACCCAAGT GTTCTTCGAT 
GAGCAAGAAG GCGGCGTAAA CCAAACGACC GAGTCGATGA CCCGCGCCTT GATCACGGAC 
GAGGCGCGCA CCGCTGGTGC CTCCATGGGT CCCGTACAGC TCGGTTACAT GGAGAAATCA 
AACGAACGTG AGGATTATCT TCCATGCTTC GTGGCTGGCG TCATCCTGAG GGAGCGACTG 
CCGGCCTTCG AGCGGATGTT GTGGCGCGCC TGCAGGGGTA ACGTCTTCCT GCGACAGGCG 
ATGATTGAGA CGCCGCTGGA GGATCCCACC AATGGCGACC AGGTGCACAA GTCCGTGTTT 
ATAATCTTCT TCCAGGGTGA CCAGCTGAAG ACGCGCGTCA AGAAGATCTG CGAGGGCTTC 
CGGGCCACGC TCTATCCTTG CCCTGAAGCT CCAGCCGATC GGCGAGAGAT GGCCATGGGT 
GTGATGACTC GCATCGAAGA TCTGAACACC GTGCTCGGCC AGACGCAGGA CCATCGCCAT 
CGTGTGCTGG TCGCTGCGGC CAAGAATCTT AAGAACTGGT TCGTCAAGGT GCGCAAGATA 
AAGGCCATCT ACCATACGCT GAATCTCTTC AATCTGGACG TGACCCAGAA GTGTCTGATC 
GCCGAGTGCT GGGTACCGCT GCTGGACATT GAAACCATCC AGCTGGCCTT GCGCCGCGGA 
ACCGAAAGAT CGGGATCGTC GGTGCCGCCA ATTCTCAACC GGATGCAGAC GTTCGAGAAT 
CCACCGACCT ACAATCGAAC GAATAAGTTT ACCAAGGCCT TTCAGGCATT GATCGATGCC 
TATGGCGTGG CCAGCTATCG GGAGATGAAT CCGGCACCCT ACACGATCAT CACCTTCCCC 
TTCCTGTTCG CCGTGATGTT TGGAGATTTG GGCCACGGAG CTATCATGGC GCTCTTTGGT 
CTCTGGATGA TTCGAAAGGA GAAGGGACTG GCGGCTCAGA AGACGGACAA CGAGATCTGG 
AACATATTCT TTGGCGGACG GTACATCATC TTCCTCATGG GCGTCTTCTC CATGTACACG 
GGTCTTATAT ACAACGATAT ATTCTCCAAG TCGCTGAATA TCTTTGGATC GCATTGGCAC 
TTATCCTACA ACAAGTCCAC CGTGATGGAG AACAAGTTCC TGCAGTTGAG CCCGAAAGGC 
GACTACGAGG GCGCTCCGTA TCCGTTCGGC ATGGATCCCA TTTGGCAGGT GGCGGGAGCC 
AATAAGATCA TCTTCCACAA CGCTTACAAG ATGAAGATCT CGATCATTTT CGGTGTTATT 
CACATGATCT TCGGTGTGGT GATGAGCTGG CACAATCATA CGTATTTCAG GAACAGGATC 
TCCCTGCTCT ACGAGTTCAT TCCCCAGCTG GTCTTCCTGC TGCTGCTTTT CTTCTACATG 
GTTTTGTTGA TGTTCATCAA GTGGATCAAG TTTGCCGCCA CCAATGATAA GCCCTACTCC 
GAAGCCTGTG CGCCTTCAAT CCTGATCACC TTTATCGACA TGGTGCTTTT TAACACGCCC 
AAGCCACCTC CGGAGAACTG TGAAACCTAT ATGTTCATGG GCCAGCACTT TATCCAGGTG 
TTATTCGTCC TGGTTGCCGT CGGCTGCATT CCCGTAATGT TGCTGGCCAA GCCGCTGCTC 
ATCATGCAGG CCCGCAAGCA GGCGAACGAA GAGGTACAGC CCATTGCAGG AGCAACTTCA 
GATGCAGAAG CCGGCGGCGT GTCCAATAGC GGCTCACACG GTGGTGGCGG TGGCCATGAG 
GAGGAAGAGG AGCTCTCTGA GATCTTCATT CACCAGAGCA TCCACACCAT CGAGTACGTC 
CTAGGTTCGG TATCCCACAC CGCTTCCTAT CTGCGATTGT GGGCGCTTTC CTTGGCCCAT 
GCCCAGTTGG CTGAGGTGCT GTGGACCATG GTCCTATCGA TTGGCTTGAA GCAGGAAGGG 
CCGGTGGGTG GCATTGTATT GACCTGCGTG TTTGCCTTCT GGGCCATTCT CACCGTTGGC 
ATTCTGGTAC TCATGGAGGG CTTGTCTGCC TTCCTGCACA CCCTGCGTCT CCATTGGGTC 
GAGTTCCAGA GCAAGTTCTA CAAGGGCCAG GGTTACGCCT TCCAACCCTT CTCGTTTGAT 
GCCATCATAG AAAACGGAGC TGCCGCCGCC GAGGAGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	Vha100-1
Entrez Gene ID	43442
Full Name	Vacuolar H[+] ATPase 100kD subunit 1
Synonyms	BcDNA:LD21248,CG1709,Dmel\CG1709,V0,V0a,V100,Vha100,v0a1,v100,vha,vha100-1
General protein information	Preferred Names Vacuolar H[+] ATPase 100kD subunit 1 Names CG1709 gene product from transcript CG1709-RF CG1709-PA CG1709-PB CG1709-PC CG1709-PD CG1709-PE CG1709-PF CG1709-PG CG1709-PI CG1709-PJ CG1709-PK V0-ATPase a1 Vha100-1-PA Vha100-1-PB Vha100-1-PC Vha100-1-PD Vha100-1-PE Vha100-1-PF Vha100-1-PG Vha100-1-PI Vha100-1-PJ Vha100-1-PK neuron-specific V[[0]] subunit a1 vacuolar ATPase V0, subunit a1 vacuolar H[+] ATPase 100kD subunit 1 vacuolar H[+] ATPase subunit 100-1
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 3R Map Location: 98F12-98F12

Homo sapiens (human)	ATP6V0A1	NP_001123493.1
Bos taurus (cattle)	ATP6V0A1	NP_777179.1
Mus musculus (house mouse)	Atp6v0a1	NP_058616.1
Saccharomyces cerevisiae (baker's yeast)	VPH1	NP_014913.3
Kluyveromyces lactis	KLLA0C07491g	XP_452533.1
Eremothecium gossypii	AGOS_ADR177C	NP_984273.2
Schizosaccharomyces pombe (fission yeast)	vph1	NP_594219.2
Gallus gallus (chicken)	ATP6V0A1	NP_990055.1
Drosophila melanogaster (fruit fly)	Vha100-1	NP_651672.1
Caenorhabditis elegans (roundworm)	unc-32	NP_001023017.1
Magnaporthe oryzae (rice blast fungus)	MGG_03947	XP_003719917.1
Arabidopsis thaliana (thale cress)	VHA-A1	NP_850122.1
Oryza sativa (rice)	Os01g0834200	NP_001044718.2
Rattus norvegicus (Norway rat)	Atp6v0a1	NP_113792.2
Neurospora crassa	NCU03463	XP_956054.1
Pan troglodytes (chimpanzee)	ATP6V0A1	XP_001165034.1
	ATP6V0A1	XP_001095161.2
Canis lupus familiaris (dog)	ATP6V0A1	XP_849622.2
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003711	XP_003436582.1
Xenopus tropicalis (tropical clawed frog)	atp6v0a1	NP_001090714.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

Vha100-1 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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