SMC3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	SMC3
Entrez Gene ID	853371
Full Name	cohesin subunit SMC3
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001181507.1	NP_012461.1	cohesin subunit SMC3

Pathways from BioSystems

Homologies

	SMC3	XP_002805863.1
Mus musculus (house mouse)	Smc3	NP_031816.2
Rattus norvegicus (Norway rat)	Smc3	NP_113771.2
Canis lupus familiaris (dog)	SMC3	XP_851818.2
Eremothecium gossypii	AGOS_AAL182W	NP_982360.1
Homo sapiens (human)	SMC3	NP_005436.1
Pan troglodytes (chimpanzee)	SMC3	XP_508031.3
Bos taurus (cattle)	SMC3	NP_776720.1
Saccharomyces cerevisiae (baker's yeast)	SMC3	NP_012461.1
Gallus gallus (chicken)	SMC3	NP_989848.1
Drosophila melanogaster (fruit fly)	Cap	NP_523374.2
Caenorhabditis elegans (roundworm)	smc-3	NP_001255118.1
Schizosaccharomyces pombe (fission yeast)	psm3	NP_593260.1
Arabidopsis thaliana (thale cress)	TTN7	NP_180285.4
Oryza sativa (rice)	Os02g0133300	NP_001045803.1
Oryza sativa (rice)	Os02g0133400	NP_001172788.1
Xenopus tropicalis (tropical clawed frog)	smc3	XP_002939544.2
Danio rerio (zebrafish)	smc3	NP_999854.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP006388	XP_316422.3
Kluyveromyces lactis	KLLA0A00286g	XP_451012.2
Magnaporthe oryzae (rice blast fungus)	MGG_04988	XP_003712488.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
Galibert F, Alexandraki D, Baur A, Boles E, Chalwatzis N, Chuat JC, Coster F, Cziepluch C, De Haan M, Domdey H, Durand P, Entian KD, Gatius M, Goffeau A, Grivell LA, Hennemann A, Herbert CJ, Heumann K, Hilger F, Hollenberg CP, Huang ME, Jacq C, Jauniaux JC, Katsoulou C, Karpfinger-Hartl L
The EMBO journal15(9)2031-49(1996 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Smc3 deacetylation by Hos1, triggered by Scc1 cleavage, facilitates efficient dissolution of sister chromatid cohesion during early metaphase.
Title: Smc3 Deacetylation by Hos1 Facilitates Efficient Dissolution of Sister Chromatid Cohesion during Early Anaphase.

A reversible association between Smc1 and Smc3 coiled coils is regulated by lysine acetylation and is required for cohesin association with the DNA.
Title: A Reversible Association between Smc Coiled Coils Is Regulated by Lysine Acetylation and Is Required for Cohesin Association with the DNA.

We identified a large number of mutations in the CC region of both Smc1 and Smc3... we introduced them to the yeast Smc1 and Smc3 CC domains and characterized the effect of these mutant alleles on cohesin's function. We identified a missense mutation in the region of the kink domain of Smc3, which was previously identified in kidney carcinoma
Title: Identification of a region in the coiled-coil domain of Smc3 that is essential for cohesin activity.

Study shows that cohesin's release from the DNA is mediated by disengagement of the Smc3/Scc1 interface involving interaction between the SMC ATPase heads and is inhibited by Smc3 acetylation.
Title: Releasing Activity Disengages Cohesin's Smc3/Scc1 Interface in a Process Blocked by Acetylation.

These results also suggest that Eco1 promotes cohesion by modulating the ATPase cycle of DNA-bound cohesin in a state that is permissive for DNA tethering and refractory to Wpl1 inhibition.
Title: The ATPases of cohesin interface with regulators to modulate cohesin-mediated DNA tethering.

The following SMC3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SMC3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi02637	NM_001181507.1 Latest version!	Saccharomyces cerevisiae S288C cohesin subunit SMC3 (SMC3), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SMC3 ( NM_001181507.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_012461.1 Saccharomyces cerevisiae S288C cohesin subunit SMC3 (SMC3), partial mRNA.

Price & Availability↑

CloneID	OSi02637
Clone ID Related Accession (Same CDS sequence)	NM_001181507.1
Accession Version	NM_001181507.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3693bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	cohesin subunit SMC3
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001142). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGTATATCA AAAGGGTGAT AATTAAGGGT TTTAAAACCT ACAGGAACGA AACCATTATT 
GATAATTTCT CGCCGCATCA GAATGTTATT ATTGGCTCCA ATGGTTCAGG TAAATCGAAT 
TTCTTCGCTG CGATTAGATT TGTTCTTAGT GACGATTACT CTAATCTTAA GAGGGAAGAA 
AGGCAAGGGC TTATCCACCA AGGTTCTGGT GGTTCAGTGA TGAGTGCCTC GGTAGAAATT 
GTGTTCCACG ATCCAGATCA TTCTATGATA TTGCCCTCCG GTGTACTTTC GAGAGGAGAT 
GACGAAGTGA CCATTAGAAG AACAGTAGGG CTGAAGAAGG ATGACTATCA ATTAAATGAC 
AGAAACGTGA CCAAAGGGGA CATAGTCAGA ATGTTGGAAA CCGCTGGTTT TTCAATGAAT 
AATCCGTATA ATATAGTACC ACAAGGTAAA ATAGTCGCCT TAACTAATGC CAAGGATAAG 
GAAAGATTGC AGTTATTGGA AGATGTTGTA GGAGCTAAGT CATTTGAAGT AAAATTGAAA 
GCTTCATTGA AAAAAATGGA GGAAACAGAG CAGAAAAAGA TTCAAATTAA CAAGGAAATG 
GGTGAACTAA ACTCTAAATT GAGTGAAATG GAACAGGAAC GTAAGGAATT AGAGAAATAC 
AACGAGTTGG AACGCAACAG AAAGATCTAC CAGTTCACAT TGTATGATCG AGAATTAAAT 
GAGGTTATCA ATCAAATGGA AAGACTGGAC GGTGATTATA ATAACACGGT ATACTCTTCC 
GAACAATACA TCCAGGAGTT GGATAAAAGA GAGGATATGA TTGACCAAGT CTCCAAAAAG 
CTATCAAGTA TTGAAGCTTC ACTGAAGATC AAAAATGCGA CTGATTTACA ACAGGCAAAA 
CTACGGGAAT CTGAGATTTC TCAGAAATTA ACAAACGTCA ACGTAAAAAT AAAGGATGTT 
CAACAGCAAA TTGAGTCAAA TGAGGAGCAG AGAAATTTGG ATAGTGCAAC TTTGAAAGAG 
ATCAAATCTA TAATTGAGCA AAGGAAACAA AAGTTGTCTA AAATTTTACC TAGATATCAA 
GAACTCACAA AGGAAGAAGC AATGTACAAA CTTCAATTGG CCTCCTTGCA ACAAAAGCAG 
AGAGATCTTA TCTTAAAGAA AGGAGAATAT GCCCGTTTTA AATCAAAGGA TGAACGCGAT 
ACGTGGATTC ATTCAGAAAT TGAAGAACTG AAATCTTCAA TACAAAACTT AAATGAACTG 
GAAAGTCAAT TGCAAATGGA TCGTACTTCT TTAAGGAAGC AGTACTCTGC GATTGATGAA 
GAGATTGAAG AGCTAATTGA TTCTATCAAT GGACCAGATA CCAAGGGTCA ATTAGAAGAT 
TTTGATTCGG AATTAATACA CCTCAAACAG AAGCTGAGTG AAAGTTTAGA CACTCGTAAG 
GAATTGTGGA GAAAGGAGCA AAAATTACAG ACAGTCTTGG AAACTTTATT AAGTGACGTC 
AATCAAAATC AAAGAAATGT TAATGAAACC ATGAGTAGAT CATTGGCAAA TGGTATAATA 
AATGTGAAAG AAATTACAGA AAAACTAAAA ATATCACCGG AATCTGTCTT TGGGACATTA 
GGTGAACTGA TTAAAGTCAA TGATAAGTAC AAGACATGTG CTGAAGTAAT TGGAGGTAAC 
TCCCTCTTCC ACATTGTCGT GGATACCGAA GAAACGGCCA CTTTGATTAT GAACGAACTA 
TATCGTATGA AGGGTGGGAG AGTGACGTTT ATTCCCTTGA ACAGGTTATC TTTGGACAGT 
GATGTTAAAT TCCCCTCAAA CACTACTACA CAAATCCAGT TTACTCCCTT GATAAAGAAG 
ATAAAATATG AACCCAGGTT TGAGAAGGCT GTCAAACACG TTTTTGGAAA AACTATTGTT 
GTCAAAGACT TAGGTCAAGG TTTAAAATTA GCTAAGAAAC ACAAACTCAA TGCCATCACT 
TTAGATGGTG ATAGAGCAGA CAAAAGAGGT GTACTTACAG GTGGGTATCT TGACCAGCAC 
AAACGAACAC GTTTGGAATC ATTGAAAAAC TTAAATGAAT CAAGAAGTCA ACACAAAAAA 
ATACTGGAAG AATTAGATTT CGTAAGAAAT GAACTAAATG ATATTGATAC TAAGATTGAT 
CAAGTAAACG GGAATATAAG AAAGGTTTCT AATGACAGGG AGTCTGTCTT AACAAATATC 
GAGGTATATA GAACATCGCT AAACACCAAA AAGAATGAAA AATTAATTCT CGAAGAATCA 
TTAAACGCAA TTATACTAAA ACTAGAAAAG CTGAATACAA ATCGTACATT TGCTCAGGAA 
AAGCTAAATA CATTCGAAAA TGATTTGCTT CAGGAGTTTG ATAGCGAACT CTCAAAAGAA 
GAAAAGGAAA GATTAGAATC TTTAACAAAG GAGATTAGTG CAGCGCACAA TAAGCTAAAT 
ATTACATCAG ACGCCCTAGA AGGTATAACT ACAACCATTG ACTCGTTGAA TGCCGAATTG 
GAGTCCAAAC TTATCCCACA AGAAAATGAT CTAGAATCCA AGATGTCAGA AGTAGGTGAC 
GCATTTATTT TTGGTCTACA AGATGAGTTG AAAGAATTAC AATTGGAGAA GGAAAGCGTT 
GAAAAGCAGC ATGAAAACGC TGTGTTGGAA CTAGGTACCG TTCAAAGAGA AATTGAAAGT 
CTCATTGCTG AAGAAACTAA TAATAAGAAA CTGCTAGAAA AAGCAAACAA TCAACAGCGA 
TTACTATTAA AGAAGCTTGA CAATTTCCAA AAAAGTGTTG AAAAAACGAT GATTAAGAAA 
ACAACTTTGG TTACTAGAAG AGAAGAATTA CAGCAAAGGA TTAGGGAAAT TGGGCTTCTG 
CCAGAGGATG CTTTAGTAAA TGATTTTAGC GACATCACAA GTGATCAGCT ATTACAAAGA 
CTGAATGATA TGAACACAGA GATTTCTGGT TTGAAGAATG TAAACAAGAG AGCTTTTGAA 
AACTTCAAAA AGTTTAACGA AAGACGCAAA GACCTTGCGG AGAGAGCTTC AGAGCTGGAT 
GAATCTAAAG ACTCAATTCA AGATCTAATT GTTAAGTTAA AACAACAAAA GGTTAACGCG 
GTTGATTCTA CTTTCCAAAA GGTTTCTGAA AACTTTGAAG CTGTTTTTGA AAGATTAGTT 
CCCAGAGGTA CTGCTAAGTT GATTATTCAC CGTAAGAATG ATAACGCTAA CGACCATGAT 
GAAAGTATTG ATGTTGACAT GGATGCAGAA TCCAATGAAA GCCAAAACGG TAAAGATAGT 
GAAATTATGT ATACAGGTGT TTCAATTTCT GTTTCCTTCA ATTCTAAACA AAACGAACAA 
CTGCATGTTG AACAGCTCTC AGGTGGTCAG AAGACTGTAT GTGCCATTGC TTTGATTCTG 
GCAATCCAAA TGGTTGACCC TGCTTCCTTC TATCTGTTTG ATGAAATTGA TGCTGCACTC 
GACAAGCAGT ATAGAACTGC AGTCGCTACA CTTCTAAAAG AACTGTCAAA AAATGCCCAG 
TTTATTTGTA CGACGTTCAG AACAGATATG TTGCAAGTTG CAGACAAATT TTTCCGTGTT 
AAGTACGAGA ATAAGATTTC CACGGTTATT GAGGTCAATA GAGAAGAAGC AATCGGATTC 
ATTAGAGGTA GCAATAAATT CGCTGAAGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_012461.1
CDS	1..3693
Translation	MYIKRVIIKGFKTYRNETIIDNFSPHQNVIIGSNGSGKSNFFAAIRFVLSDDYSNLKREERQGLIHQGSGGSVMSASVEIVFHDPDHSMILPSGVLSRGDDEVTIRRTVGLKKDDYQLNDRNVTKGDIVRMLETAGFSMNNPYNIVPQGKIVALTNAKDKERLQLLEDVVGAKSFEVKLKASLKKMEETEQKKIQINKEMGELNSKLSEMEQERKELEKYNELERNRKIYQFTLYDRELNEVINQMERLDGDYNNTVYSSEQYIQELDKREDMIDQVSKKLSSIEASLKIKNATDLQQAKLRESEISQKLTNVNVKIKDVQQQIESNEEQRNLDSATLKEIKSIIEQRKQKLSKILPRYQELTKEEAMYKLQLASLQQKQRDLILKKGEYARFKSKDERDTWIHSEIEELKSSIQNLNELESQLQMDRTSLRKQYSAIDEEIEELIDSINGPDTKGQLEDFDSELIHLKQKLSESLDTRKELWRKEQKLQTVLETLLSDVNQNQRNVNETMSRSLANGIINVKEITEKLKISPESVFGTLGELIKVNDKYKTCAEVIGGNSLFHIVVDTEETATLIMNELYRMKGGRVTFIPLNRLSLDSDVKFPSNTTTQIQFTPLIKKIKYEPRFEKAVKHVFGKTIVVKDLGQGLKLAKKHKLNAITLDGDRADKRGVLTGGYLDQHKRTRLESLKNLNESRSQHKKILEELDFVRNELNDIDTKIDQVNGNIRKVSNDRESVLTNIEVYRTSLNTKKNEKLILEESLNAIILKLEKLNTNRTFAQEKLNTFENDLLQEFDSELSKEEKERLESLTKEISAAHNKLNITSDALEGITTTIDSLNAELESKLIPQENDLESKMSEVGDAFIFGLQDELKELQLEKESVEKQHENAVLELGTVQREIESLIAEETNNKKLLEKANNQQRLLLKKLDNFQKSVEKTMIKKTTLVTRREELQQRIREIGLLPEDALVNDFSDITSDQLLQRLNDMNTEISGLKNVNKRAFENFKKFNERRKDLAERASELDESKDSIQDLIVKLKQQKVNAVDSTFQKVSENFEAVFERLVPRGTAKLIIHRKNDNANDHDESIDVDMDAESNESQNGKDSEIMYTGVSISVSFNSKQNEQLHVEQLSGGQKTVCAIALILAIQMVDPASFYLFDEIDAALDKQYRTAVATLLKELSKNAQFICTTFRTDMLQVADKFFRVKYENKISTVIEVNREEAIGFIRGSNKFAEV

Target ORF information:

RefSeq Version	NM_001181507.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C cohesin subunit SMC3 (SMC3), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001181507.1

ORF Insert Sequence:

ATGTATATCA AAAGGGTGAT AATTAAGGGT TTTAAAACCT ACAGGAACGA AACCATTATT 
GATAATTTCT CGCCGCATCA GAATGTTATT ATTGGCTCCA ATGGTTCAGG TAAATCGAAT 
TTCTTCGCTG CGATTAGATT TGTTCTTAGT GACGATTACT CTAATCTTAA GAGGGAAGAA 
AGGCAAGGGC TTATCCACCA AGGTTCTGGT GGTTCAGTGA TGAGTGCCTC GGTAGAAATT 
GTGTTCCACG ATCCAGATCA TTCTATGATA TTGCCCTCCG GTGTACTTTC GAGAGGAGAT 
GACGAAGTGA CCATTAGAAG AACAGTAGGG CTGAAGAAGG ATGACTATCA ATTAAATGAC 
AGAAACGTGA CCAAAGGGGA CATAGTCAGA ATGTTGGAAA CCGCTGGTTT TTCAATGAAT 
AATCCGTATA ATATAGTACC ACAAGGTAAA ATAGTCGCCT TAACTAATGC CAAGGATAAG 
GAAAGATTGC AGTTATTGGA AGATGTTGTA GGAGCTAAGT CATTTGAAGT AAAATTGAAA 
GCTTCATTGA AAAAAATGGA GGAAACAGAG CAGAAAAAGA TTCAAATTAA CAAGGAAATG 
GGTGAACTAA ACTCTAAATT GAGTGAAATG GAACAGGAAC GTAAGGAATT AGAGAAATAC 
AACGAGTTGG AACGCAACAG AAAGATCTAC CAGTTCACAT TGTATGATCG AGAATTAAAT 
GAGGTTATCA ATCAAATGGA AAGACTGGAC GGTGATTATA ATAACACGGT ATACTCTTCC 
GAACAATACA TCCAGGAGTT GGATAAAAGA GAGGATATGA TTGACCAAGT CTCCAAAAAG 
CTATCAAGTA TTGAAGCTTC ACTGAAGATC AAAAATGCGA CTGATTTACA ACAGGCAAAA 
CTACGGGAAT CTGAGATTTC TCAGAAATTA ACAAACGTCA ACGTAAAAAT AAAGGATGTT 
CAACAGCAAA TTGAGTCAAA TGAGGAGCAG AGAAATTTGG ATAGTGCAAC TTTGAAAGAG 
ATCAAATCTA TAATTGAGCA AAGGAAACAA AAGTTGTCTA AAATTTTACC TAGATATCAA 
GAACTCACAA AGGAAGAAGC AATGTACAAA CTTCAATTGG CCTCCTTGCA ACAAAAGCAG 
AGAGATCTTA TCTTAAAGAA AGGAGAATAT GCCCGTTTTA AATCAAAGGA TGAACGCGAT 
ACGTGGATTC ATTCAGAAAT TGAAGAACTG AAATCTTCAA TACAAAACTT AAATGAACTG 
GAAAGTCAAT TGCAAATGGA TCGTACTTCT TTAAGGAAGC AGTACTCTGC GATTGATGAA 
GAGATTGAAG AGCTAATTGA TTCTATCAAT GGACCAGATA CCAAGGGTCA ATTAGAAGAT 
TTTGATTCGG AATTAATACA CCTCAAACAG AAGCTGAGTG AAAGTTTAGA CACTCGTAAG 
GAATTGTGGA GAAAGGAGCA AAAATTACAG ACAGTCTTGG AAACTTTATT AAGTGACGTC 
AATCAAAATC AAAGAAATGT TAATGAAACC ATGAGTAGAT CATTGGCAAA TGGTATAATA 
AATGTGAAAG AAATTACAGA AAAACTAAAA ATATCACCGG AATCTGTCTT TGGGACATTA 
GGTGAACTGA TTAAAGTCAA TGATAAGTAC AAGACATGTG CTGAAGTAAT TGGAGGTAAC 
TCCCTCTTCC ACATTGTCGT GGATACCGAA GAAACGGCCA CTTTGATTAT GAACGAACTA 
TATCGTATGA AGGGTGGGAG AGTGACGTTT ATTCCCTTGA ACAGGTTATC TTTGGACAGT 
GATGTTAAAT TCCCCTCAAA CACTACTACA CAAATCCAGT TTACTCCCTT GATAAAGAAG 
ATAAAATATG AACCCAGGTT TGAGAAGGCT GTCAAACACG TTTTTGGAAA AACTATTGTT 
GTCAAAGACT TAGGTCAAGG TTTAAAATTA GCTAAGAAAC ACAAACTCAA TGCCATCACT 
TTAGATGGTG ATAGAGCAGA CAAAAGAGGT GTACTTACAG GTGGGTATCT TGACCAGCAC 
AAACGAACAC GTTTGGAATC ATTGAAAAAC TTAAATGAAT CAAGAAGTCA ACACAAAAAA 
ATACTGGAAG AATTAGATTT CGTAAGAAAT GAACTAAATG ATATTGATAC TAAGATTGAT 
CAAGTAAACG GGAATATAAG AAAGGTTTCT AATGACAGGG AGTCTGTCTT AACAAATATC 
GAGGTATATA GAACATCGCT AAACACCAAA AAGAATGAAA AATTAATTCT CGAAGAATCA 
TTAAACGCAA TTATACTAAA ACTAGAAAAG CTGAATACAA ATCGTACATT TGCTCAGGAA 
AAGCTAAATA CATTCGAAAA TGATTTGCTT CAGGAGTTTG ATAGCGAACT CTCAAAAGAA 
GAAAAGGAAA GATTAGAATC TTTAACAAAG GAGATTAGTG CAGCGCACAA TAAGCTAAAT 
ATTACATCAG ACGCCCTAGA AGGTATAACT ACAACCATTG ACTCGTTGAA TGCCGAATTG 
GAGTCCAAAC TTATCCCACA AGAAAATGAT CTAGAATCCA AGATGTCAGA AGTAGGTGAC 
GCATTTATTT TTGGTCTACA AGATGAGTTG AAAGAATTAC AATTGGAGAA GGAAAGCGTT 
GAAAAGCAGC ATGAAAACGC TGTGTTGGAA CTAGGTACCG TTCAAAGAGA AATTGAAAGT 
CTCATTGCTG AAGAAACTAA TAATAAGAAA CTGCTAGAAA AAGCAAACAA TCAACAGCGA 
TTACTATTAA AGAAGCTTGA CAATTTCCAA AAAAGTGTTG AAAAAACGAT GATTAAGAAA 
ACAACTTTGG TTACTAGAAG AGAAGAATTA CAGCAAAGGA TTAGGGAAAT TGGGCTTCTG 
CCAGAGGATG CTTTAGTAAA TGATTTTAGC GACATCACAA GTGATCAGCT ATTACAAAGA 
CTGAATGATA TGAACACAGA GATTTCTGGT TTGAAGAATG TAAACAAGAG AGCTTTTGAA 
AACTTCAAAA AGTTTAACGA AAGACGCAAA GACCTTGCGG AGAGAGCTTC AGAGCTGGAT 
GAATCTAAAG ACTCAATTCA AGATCTAATT GTTAAGTTAA AACAACAAAA GGTTAACGCG 
GTTGATTCTA CTTTCCAAAA GGTTTCTGAA AACTTTGAAG CTGTTTTTGA AAGATTAGTT 
CCCAGAGGTA CTGCTAAGTT GATTATTCAC CGTAAGAATG ATAACGCTAA CGACCATGAT 
GAAAGTATTG ATGTTGACAT GGATGCAGAA TCCAATGAAA GCCAAAACGG TAAAGATAGT 
GAAATTATGT ATACAGGTGT TTCAATTTCT GTTTCCTTCA ATTCTAAACA AAACGAACAA 
CTGCATGTTG AACAGCTCTC AGGTGGTCAG AAGACTGTAT GTGCCATTGC TTTGATTCTG 
GCAATCCAAA TGGTTGACCC TGCTTCCTTC TATCTGTTTG ATGAAATTGA TGCTGCACTC 
GACAAGCAGT ATAGAACTGC AGTCGCTACA CTTCTAAAAG AACTGTCAAA AAATGCCCAG 
TTTATTTGTA CGACGTTCAG AACAGATATG TTGCAAGTTG CAGACAAATT TTTCCGTGTT 
AAGTACGAGA ATAAGATTTC CACGGTTATT GAGGTCAATA GAGAAGAAGC AATCGGATTC 
ATTAGAGGTA GCAATAAATT CGCTGAAGTC TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Complete nucleotide sequence of Saccharomyces cerevisiae chromosome X.
The EMBO journal15(9)2031-49(1996 May)
Galibert F,Alexandraki D,Baur A,Boles E,Chalwatzis N,Chuat JC,Coster F,Cziepluch C,De Haan M,Domdey H,Durand P,Entian KD,Gatius M,Goffeau A,Grivell LA,Hennemann A,Herbert CJ,Heumann K,Hilger F,Hollenberg CP,Huang ME,Jacq C,Jauniaux JC,Katsoulou C,Karpfinger-Hartl L

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SMC3 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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