ACT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene
Clones
gRNAs

Summary

Gene Symbol	ACT1
Entrez Gene ID	850504
Full Name	actin
Synonyms	ABY1,END7
Gene Type	protein-coding
Organism	Saccharomyces cerevisiae S288C

mRNA and Protein(s)

mRNA	Protein	Name
NM_001179927.1	NP_116614.1	actin

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	act-1	NP_505819.1
Schizosaccharomyces pombe (fission yeast)	act1	NP_595618.1
Gallus gallus (chicken)	ACTG1	NP_001007825.1
Anopheles gambiae (African malaria mosquito)	ACT5C_ANOGA	XP_003437050.1
Eremothecium gossypii	AGOS_ABR222W	NP_983171.1
Arabidopsis thaliana (thale cress)	ACT8	NP_175350.1
Oryza sativa (rice)	Os03g0718100	NP_001051086.1
Mus musculus (house mouse)	Actg1	NP_033739.1
Rattus norvegicus (Norway rat)	Actg1	NP_001120921.1
Danio rerio (zebrafish)	actb1	NP_571106.1
Caenorhabditis elegans (roundworm)	act-3	NP_505817.1
Caenorhabditis elegans (roundworm)	act-2	NP_505818.1
Kluyveromyces lactis	KLLA0D05357g	XP_453299.1
Magnaporthe oryzae (rice blast fungus)	MGG_03982	XP_003719871.1
Neurospora crassa	NCU04173	XP_961133.2
Homo sapiens (human)	ACTG1	NP_001605.1
Bos taurus (cattle)	ACTG1	NP_001028790.1
Danio rerio (zebrafish)	actb2	NP_853632.3
Drosophila melanogaster (fruit fly)	Act5C	NP_001014726.1
Saccharomyces cerevisiae (baker's yeast)	ACT1	NP_116614.1
	ACTG1	XP_001110892.2
Caenorhabditis elegans (roundworm)	act-4	NP_508841.1
Arabidopsis thaliana (thale cress)	ACT2	NP_188508.1
Xenopus tropicalis (tropical clawed frog)	actg1	NP_989332.1

Gene Ontology
Bibliography

Related articles in PubMed

Life with 6000 genes.
Goffeau A, Barrell BG, Bussey H, Davis RW, Dujon B, Feldmann H, Galibert F, Hoheisel JD, Jacq C, Johnston M, Louis EJ, Mewes HW, Murakami Y, Philippsen P, Tettelin H, Oliver SG
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Murakami Y, Naitou M, Hagiwara H, Shibata T, Ozawa M, Sasanuma S, Sasanuma M, Tsuchiya Y, Soeda E, Yokoyama K
Nature genetics10(3)261-8(1995 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Act1 protein affects tombusvirus recombination in yeast .
Title: Screening a yeast library of temperature-sensitive mutants reveals a role for actin in tombusvirus RNA recombination.

Using multi-color total internal reflection fluorescence microscopy, the study shows that Crn1 enhances Cof1-mediated severing by accelerating Cof1 binding to actin filament sides.
Title: Coronin Enhances Actin Filament Severing by Recruiting Cofilin to Filament Sides and Altering F-Actin Conformation.

Heat shock-induced processing of actin assembly protein Lsb1 is involved in prion inheritance.
Title: Stress-dependent proteolytic processing of the actin assembly protein Lsb1 modulates a yeast prion.

Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2alpha phosphorylation.
Title: Translation elongation factor 1A mutants with altered actin bundling activity show reduced aminoacyl-tRNA binding and alter initiation via eIF2? phosphorylation.

Data suggest that a glycolytic multi-enzyme complex assembles in cytoplasm of S. cerevisiae in association with F-actin (filamentous actin) but not in association with G-actin (monomeric globular actin).
Title: A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin.

The following ACT1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACT1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSi00197	NM_001179927.1 Latest version!	Saccharomyces cerevisiae S288C actin (ACT1), partial mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACT1 ( NM_001179927.1 ) cDNA ORF clone, Saccharomyces cerevisiae S288C -> NP_116614.1 Saccharomyces cerevisiae S288C actin (ACT1), partial mRNA.

Price & Availability↑

CloneID	OSi00197
Clone ID Related Accession (Same CDS sequence)	NM_001179927.1
Accession Version	NM_001179927.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-31
Organism	Saccharomyces cerevisiae S288C
Product	actin
Comment	Comment: REVIEWED REFSEQ: This record has been curated by SGD. This record is derived from an annotated genomic sequence (NC_001138). ##Genome-Annotation-Data-START## Annotation Provider :: SGD Annotation Status :: Full Annotation Annotation Version :: R64-2-1 URL :: http://www.yeastgenome.org/ ##Genome-Annotation-Data-END## COMPLETENESS: incomplete on both ends.

ATGGATTCTG AGGTTGCTGC TTTGGTTATT GATAACGGTT CTGGTATGTG TAAAGCCGGT 
TTTGCCGGTG ACGACGCTCC TCGTGCTGTC TTCCCATCTA TCGTCGGTAG ACCAAGACAC 
CAAGGTATCA TGGTCGGTAT GGGTCAAAAA GACTCCTACG TTGGTGATGA AGCTCAATCC 
AAGAGAGGTA TCTTGACTTT ACGTTACCCA ATTGAACACG GTATTGTCAC CAACTGGGAC 
GATATGGAAA AGATCTGGCA TCATACCTTC TACAACGAAT TGAGAGTTGC CCCAGAAGAA 
CACCCTGTTC TTTTGACTGA AGCTCCAATG AACCCTAAAT CAAACAGAGA AAAGATGACT 
CAAATTATGT TTGAAACTTT CAACGTTCCA GCCTTCTACG TTTCCATCCA AGCCGTTTTG 
TCCTTGTACT CTTCCGGTAG AACTACTGGT ATTGTTTTGG ATTCCGGTGA TGGTGTTACT 
CACGTCGTTC CAATTTACGC TGGTTTCTCT CTACCTCACG CCATTTTGAG AATCGATTTG 
GCCGGTAGAG ATTTGACTGA CTACTTGATG AAGATCTTGA GTGAACGTGG TTACTCTTTC 
TCCACCACTG CTGAAAGAGA AATTGTCCGT GACATCAAGG AAAAACTATG TTACGTCGCC 
TTGGACTTCG AACAAGAAAT GCAAACCGCT GCTCAATCTT CTTCAATTGA AAAATCCTAC 
GAACTTCCAG ATGGTCAAGT CATCACTATT GGTAACGAAA GATTCAGAGC CCCAGAAGCT 
TTGTTCCATC CTTCTGTTTT GGGTTTGGAA TCTGCCGGTA TTGACCAAAC TACTTACAAC 
TCCATCATGA AGTGTGATGT CGATGTCCGT AAGGAATTAT ACGGTAACAT CGTTATGTCC 
GGTGGTACCA CCATGTTCCC AGGTATTGCC GAAAGAATGC AAAAGGAAAT CACCGCTTTG 
GCTCCATCTT CCATGAAGGT CAAGATCATT GCTCCTCCAG AAAGAAAGTA CTCCGTCTGG 
ATTGGTGGTT CTATCTTGGC TTCTTTGACT ACCTTCCAAC AAATGTGGAT CTCAAAACAA 
GAATACGACG AAAGTGGTCC ATCTATCGTT CACCACAAGT GTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_116614.1
CDS	1..1128
Translation	MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF

Target ORF information:

RefSeq Version	NM_001179927.1
Organism	Saccharomyces cerevisiae S288C
Definition	Saccharomyces cerevisiae S288C actin (ACT1), partial mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001179927.1

ORF Insert Sequence:

ATGGATTCTG AGGTTGCTGC TTTGGTTATT GATAACGGTT CTGGTATGTG TAAAGCCGGT 
TTTGCCGGTG ACGACGCTCC TCGTGCTGTC TTCCCATCTA TCGTCGGTAG ACCAAGACAC 
CAAGGTATCA TGGTCGGTAT GGGTCAAAAA GACTCCTACG TTGGTGATGA AGCTCAATCC 
AAGAGAGGTA TCTTGACTTT ACGTTACCCA ATTGAACACG GTATTGTCAC CAACTGGGAC 
GATATGGAAA AGATCTGGCA TCATACCTTC TACAACGAAT TGAGAGTTGC CCCAGAAGAA 
CACCCTGTTC TTTTGACTGA AGCTCCAATG AACCCTAAAT CAAACAGAGA AAAGATGACT 
CAAATTATGT TTGAAACTTT CAACGTTCCA GCCTTCTACG TTTCCATCCA AGCCGTTTTG 
TCCTTGTACT CTTCCGGTAG AACTACTGGT ATTGTTTTGG ATTCCGGTGA TGGTGTTACT 
CACGTCGTTC CAATTTACGC TGGTTTCTCT CTACCTCACG CCATTTTGAG AATCGATTTG 
GCCGGTAGAG ATTTGACTGA CTACTTGATG AAGATCTTGA GTGAACGTGG TTACTCTTTC 
TCCACCACTG CTGAAAGAGA AATTGTCCGT GACATCAAGG AAAAACTATG TTACGTCGCC 
TTGGACTTCG AACAAGAAAT GCAAACCGCT GCTCAATCTT CTTCAATTGA AAAATCCTAC 
GAACTTCCAG ATGGTCAAGT CATCACTATT GGTAACGAAA GATTCAGAGC CCCAGAAGCT 
TTGTTCCATC CTTCTGTTTT GGGTTTGGAA TCTGCCGGTA TTGACCAAAC TACTTACAAC 
TCCATCATGA AGTGTGATGT CGATGTCCGT AAGGAATTAT ACGGTAACAT CGTTATGTCC 
GGTGGTACCA CCATGTTCCC AGGTATTGCC GAAAGAATGC AAAAGGAAAT CACCGCTTTG 
GCTCCATCTT CCATGAAGGT CAAGATCATT GCTCCTCCAG AAAGAAAGTA CTCCGTCTGG 
ATTGGTGGTT CTATCTTGGC TTCTTTGACT ACCTTCCAAC AAATGTGGAT CTCAAAACAA 
GAATACGACG AAAGTGGTCC ATCTATCGTT CACCACAAGT GTTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Life with 6000 genes.
Science (New York, N.Y.)274(5287)546, 563-7(1996 Oct)
Goffeau A,Barrell BG,Bussey H,Davis RW,Dujon B,Feldmann H,Galibert F,Hoheisel JD,Jacq C,Johnston M,Louis EJ,Mewes HW,Murakami Y,Philippsen P,Tettelin H,Oliver SG

Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae.
Nature genetics10(3)261-8(1995 Jul)
Murakami Y,Naitou M,Hagiwara H,Shibata T,Ozawa M,Sasanuma S,Sasanuma M,Tsuchiya Y,Soeda E,Yokoyama K

- Most Popular Services
  - 人工遺伝子合成& DNA合成-納期保証
- Services & Products

見積依頼

Eメール

japanmarket@genscript.com

電話

03-6811-6572

FAX

03-6811-6573
050-3174-7589(受注専用)

オンラインフォーム

オンライン注文

ACT1 cDNA ORF clone, Saccharomyces cerevisiae S288C

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Most Popular Services

Services & Products

見積依頼

Eメール

電話

FAX

オンラインフォーム

サポート

会社情報

サポート

会社情報