Actg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actg1
Entrez Gene ID	287876
Full Name	actin, gamma 1
Synonyms	Actg
General protein information	Preferred Names actin, gamma 1 Names actin, cytoplasmic 2 actin, gamma, cytoplasmic 1 gamma-actin
Gene Type	protein-coding
Organism	Rattus norvegicus(Norway rat)
Genome	Chromosome: 10 Map Location: 10q32.3

mRNA and Protein(s)

mRNA	Protein	Name
NM_001127449.1	NP_001120921.1	actin, cytoplasmic 2
XM_017597166.1	XP_017452655.1	actin, cytoplasmic 2 isoform X1
XM_017597167.1	XP_017452656.1	actin, cytoplasmic 2 isoform X2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	act-1	NP_505819.1
Schizosaccharomyces pombe (fission yeast)	act1	NP_595618.1
Gallus gallus (chicken)	ACTG1	NP_001007825.1
Anopheles gambiae (African malaria mosquito)	ACT5C_ANOGA	XP_003437050.1
Eremothecium gossypii	AGOS_ABR222W	NP_983171.1
Arabidopsis thaliana (thale cress)	ACT8	NP_175350.1
Oryza sativa (rice)	Os03g0718100	NP_001051086.1
Mus musculus (house mouse)	Actg1	NP_033739.1
Rattus norvegicus (Norway rat)	Actg1	NP_001120921.1
Danio rerio (zebrafish)	actb1	NP_571106.1
Caenorhabditis elegans (roundworm)	act-3	NP_505817.1
Caenorhabditis elegans (roundworm)	act-2	NP_505818.1
Kluyveromyces lactis	KLLA0D05357g	XP_453299.1
Magnaporthe oryzae (rice blast fungus)	MGG_03982	XP_003719871.1
Neurospora crassa	NCU04173	XP_961133.2
Homo sapiens (human)	ACTG1	NP_001605.1
Bos taurus (cattle)	ACTG1	NP_001028790.1
Danio rerio (zebrafish)	actb2	NP_853632.3
Drosophila melanogaster (fruit fly)	Act5C	NP_001014726.1
Saccharomyces cerevisiae (baker's yeast)	ACT1	NP_116614.1
	ACTG1	XP_001110892.2
Caenorhabditis elegans (roundworm)	act-4	NP_508841.1
Arabidopsis thaliana (thale cress)	ACT2	NP_188508.1
Xenopus tropicalis (tropical clawed frog)	actg1	NP_989332.1

Gene Ontology
Bibliography

Related articles in PubMed

A recurrent de novo mutation in ACTG1 causes isolated ocular coloboma.
Rainger J, Williamson KA, Soares DC, Truch J, Kurian D, Gillessen-Kaesbach G, Seawright A, Prendergast J, Halachev M, Wheeler A, McTeir L, Gill AC, van Heyningen V, Davey MG, , FitzPatrick DR
Human mutation38(8)942-946(2017 Aug)

Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.
Wu XS, Lee SH, Sheng J, Zhang Z, Zhao WD, Wang D, Jin Y, Charnay P, Ervasti JM, Wu LG
Neuron92(5)1020-1035(2016 Dec)

Widespread macromolecular interaction perturbations in human genetic disorders.
Sahni N, Yi S, Taipale M, Fuxman Bass JI, Coulombe-Huntington J, Yang F, Peng J, Weile J, Karras GI, Wang Y, Kov?cs IA, Kamburov A, Krykbaeva I, Lam MH, Tucker G, Khurana V, Sharma A, Liu YY, Yachie N, Zhong Q, Shen Y, Palagi A, San-Miguel A, Fan C, Balcha D, Dricot A, Jordan DM, Walsh JM, Shah AA, Yang X, Stoyanova AK, Leighton A, Calderwood MA, Jacob Y, Cusick ME, Salehi-Ashtiani K, Whitesell LJ, Sunyaev S, Berger B, Barab?si AL, Charloteaux B, Hill DE, Hao T, Roth FP, Xia Y, Walhout AJM, Lindquist S, Vidal M
Cell161(3)647-660(2015 Apr)

Possible role of non-muscle alpha-actinins in muscle cell mechanosensitivity.
Ogneva IV, Biryukov NS, Leinsoo TA, Larina IM
PloS one9(4)e96395(2014)

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Zanivan S, Maione F, Hein MY, Hern?ndez-Fernaud JR, Ostasiewicz P, Giraudo E, Mann M
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Gamma-actin is a Z disc component in skeletal myofibers.
Title: Evidence for gamma-actin as a Z disc component in skeletal myofibers.

The following Actg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ORa01619	NM_001127449.1 Latest version!	Rattus norvegicus actin, gamma 1 (Actg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa01619	XM_017597166.1 Latest version!	Rattus norvegicus actin, gamma 1 (Actg1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$188.30 $269.00
ORa57270	XM_017597167.1 Latest version!	Rattus norvegicus actin, gamma 1 (Actg1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actg1 ( NM_001127449.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> NP_001120921.1 Rattus norvegicus actin, gamma 1 (Actg1), mRNA.

Price & Availability↑

CloneID	ORa01619
Clone ID Related Accession (Same CDS sequence)	NM_001127449.1 , XM_017597166.1
Accession Version	NM_001127449.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-24
Organism	Rattus norvegicus(Norway rat)
Product	actin, cytoplasmic 2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CK595239.1, CK364554.1 and CF110974.1. On or before May 21, 2008 this sequence version replaced XM_001076100.1, XM_001058533.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN01906347, SAMN01906348 [ECO:0000348] ##Evidence-Data-END##

ATGGAAGAAG AAATCGCCGC CCTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGC 
TTTGCTGGGG ACGACGCCCC CAGGGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCCGACAC 
CAGGGTGTCA TGGTGGGCAT GGGCCAGAAA GACTCGTACG TGGGTGATGA GGCCCAGAGC 
AAGAGGGGTA TTCTGACCCT GAAGTACCCT ATTGAGCACG GCATTGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGTGTGGC CCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTC 
TCCTTGTATG CATCTGGGCG TACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAAAGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCTTTGGA GAAGAGTTAT 
GAGCTGCCTG ATGGGCAGGT GATCACCATT GGCAATGAGC GCTTCCGGTG TCCAGAGGCT 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGATGT GGACATCCGC AAAGACCTGT ATGCCAACAC AGTGCTGTCT 
GGTGGTACCA CCATGTATCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTG 
GCTCCCAGCA CAATGAAGAT TAAGATCATT GCTCCTCCTG AACGCAAGTA CTCAGTCTGG 
ATTGGCGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCAGGCCC CTCCATTGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001120921.1
CDS	75..1202
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001127449.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin, gamma 1 (Actg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001127449.1

ORF Insert Sequence:

ATGGAAGAAG AAATCGCCGC CCTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGC 
TTTGCTGGGG ACGACGCCCC CAGGGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCCGACAC 
CAGGGTGTCA TGGTGGGCAT GGGCCAGAAA GACTCGTACG TGGGTGATGA GGCCCAGAGC 
AAGAGGGGTA TTCTGACCCT GAAGTACCCT ATTGAGCACG GCATTGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGTGTGGC CCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTC 
TCCTTGTATG CATCTGGGCG TACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAAAGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCTTTGGA GAAGAGTTAT 
GAGCTGCCTG ATGGGCAGGT GATCACCATT GGCAATGAGC GCTTCCGGTG TCCAGAGGCT 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGATGT GGACATCCGC AAAGACCTGT ATGCCAACAC AGTGCTGTCT 
GGTGGTACCA CCATGTATCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTG 
GCTCCCAGCA CAATGAAGAT TAAGATCATT GCTCCTCCTG AACGCAAGTA CTCAGTCTGG 
ATTGGCGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCAGGCCC CTCCATTGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actg1 ( XM_017597166.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452655.1 Rattus norvegicus actin, gamma 1 (Actg1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ORa01619
Clone ID Related Accession (Same CDS sequence)	NM_001127449.1 , XM_017597166.1
Accession Version	XM_017597166.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	actin, cytoplasmic 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAGAAG AAATCGCCGC CCTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGC 
TTTGCTGGGG ACGACGCCCC CAGGGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCCGACAC 
CAGGGTGTCA TGGTGGGCAT GGGCCAGAAA GACTCGTACG TGGGTGATGA GGCCCAGAGC 
AAGAGGGGTA TTCTGACCCT GAAGTACCCT ATTGAGCACG GCATTGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGTGTGGC CCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTC 
TCCTTGTATG CATCTGGGCG TACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAAAGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCTTTGGA GAAGAGTTAT 
GAGCTGCCTG ATGGGCAGGT GATCACCATT GGCAATGAGC GCTTCCGGTG TCCAGAGGCT 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGATGT GGACATCCGC AAAGACCTGT ATGCCAACAC AGTGCTGTCT 
GGTGGTACCA CCATGTATCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTG 
GCTCCCAGCA CAATGAAGAT TAAGATCATT GCTCCTCCTG AACGCAAGTA CTCAGTCTGG 
ATTGGCGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCAGGCCC CTCCATTGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452655.1
CDS	26..1153
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	XM_017597166.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin, gamma 1 (Actg1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597166.1

ORF Insert Sequence:

ATGGAAGAAG AAATCGCCGC CCTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGC 
TTTGCTGGGG ACGACGCCCC CAGGGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCCGACAC 
CAGGGTGTCA TGGTGGGCAT GGGCCAGAAA GACTCGTACG TGGGTGATGA GGCCCAGAGC 
AAGAGGGGTA TTCTGACCCT GAAGTACCCT ATTGAGCACG GCATTGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGTGTGGC CCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTC 
TCCTTGTATG CATCTGGGCG TACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAAAGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCTTTGGA GAAGAGTTAT 
GAGCTGCCTG ATGGGCAGGT GATCACCATT GGCAATGAGC GCTTCCGGTG TCCAGAGGCT 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGATGT GGACATCCGC AAAGACCTGT ATGCCAACAC AGTGCTGTCT 
GGTGGTACCA CCATGTATCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTG 
GCTCCCAGCA CAATGAAGAT TAAGATCATT GCTCCTCCTG AACGCAAGTA CTCAGTCTGG 
ATTGGCGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGACG AGTCAGGCCC CTCCATTGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actg1 ( XM_017597167.1 ) cDNA ORF clone, Rattus norvegicus(Norway rat) -> XP_017452656.1 Rattus norvegicus actin, gamma 1 (Actg1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ORa57270
Clone ID Related Accession (Same CDS sequence)	XM_017597167.1
Accession Version	XM_017597167.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-07-25
Organism	Rattus norvegicus(Norway rat)
Product	actin, cytoplasmic 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_005109.4) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Rattus norvegicus Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTTTGAAA CCTTCAATAC CCCAGCCATG TACGTGGCCA TTCAGGCGGT GCTCTCCTTG 
TATGCATCTG GGCGTACCAC TGGCATTGTC ATGGACTCTG GTGACGGGGT CACACACACA 
GTGCCCATCT ATGAGGGCTA CGCCCTTCCC CACGCCATCT TGCGTCTGGA CCTGGCTGGC 
CGGGACCTGA CAGACTACCT CATGAAGATC CTGACTGAAA GGGGCTACAG CTTTACCACC 
ACTGCTGAGA GGGAAATTGT TCGTGACATA AAGGAGAAGC TGTGCTATGT TGCCCTGGAT 
TTTGAGCAAG AAATGGCTAC TGCTGCATCA TCTTCCTCTT TGGAGAAGAG TTATGAGCTG 
CCTGATGGGC AGGTGATCAC CATTGGCAAT GAGCGCTTCC GGTGTCCAGA GGCTCTCTTC 
CAGCCTTCCT TCCTGGGCAT GGAGTCCTGT GGCATCCACG AGACCACCTT CAACTCCATC 
ATGAAGTGTG ATGTGGACAT CCGCAAAGAC CTGTATGCCA ACACAGTGCT GTCTGGTGGT 
ACCACCATGT ATCCAGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTGGCTCCC 
AGCACAATGA AGATTAAGAT CATTGCTCCT CCTGAACGCA AGTACTCAGT CTGGATTGGC 
GGCTCCATCC TGGCCTCACT GTCCACCTTC CAGCAGATGT GGATCAGCAA GCAGGAGTAT 
GACGAGTCAG GCCCCTCCAT TGTCCACCGC AAATGCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017452656.1
CDS	560..1321
Translation	MFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	XM_017597167.1
Organism	Rattus norvegicus(Norway rat)
Definition	Rattus norvegicus actin, gamma 1 (Actg1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017597167.1

ORF Insert Sequence:

ATGTTTGAAA CCTTCAATAC CCCAGCCATG TACGTGGCCA TTCAGGCGGT GCTCTCCTTG 
TATGCATCTG GGCGTACCAC TGGCATTGTC ATGGACTCTG GTGACGGGGT CACACACACA 
GTGCCCATCT ATGAGGGCTA CGCCCTTCCC CACGCCATCT TGCGTCTGGA CCTGGCTGGC 
CGGGACCTGA CAGACTACCT CATGAAGATC CTGACTGAAA GGGGCTACAG CTTTACCACC 
ACTGCTGAGA GGGAAATTGT TCGTGACATA AAGGAGAAGC TGTGCTATGT TGCCCTGGAT 
TTTGAGCAAG AAATGGCTAC TGCTGCATCA TCTTCCTCTT TGGAGAAGAG TTATGAGCTG 
CCTGATGGGC AGGTGATCAC CATTGGCAAT GAGCGCTTCC GGTGTCCAGA GGCTCTCTTC 
CAGCCTTCCT TCCTGGGCAT GGAGTCCTGT GGCATCCACG AGACCACCTT CAACTCCATC 
ATGAAGTGTG ATGTGGACAT CCGCAAAGAC CTGTATGCCA ACACAGTGCT GTCTGGTGGT 
ACCACCATGT ATCCAGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTGGCTCCC 
AGCACAATGA AGATTAAGAT CATTGCTCCT CCTGAACGCA AGTACTCAGT CTGGATTGGC 
GGCTCCATCC TGGCCTCACT GTCCACCTTC CAGCAGATGT GGATCAGCAA GCAGGAGTAT 
GACGAGTCAG GCCCCTCCAT TGTCCACCGC AAATGCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

A recurrent de novo mutation in ACTG1 causes isolated ocular coloboma.
Human mutation38(8)942-946(2017 Aug)
Rainger J,Williamson KA,Soares DC,Truch J,Kurian D,Gillessen-Kaesbach G,Seawright A,Prendergast J,Halachev M,Wheeler A,McTeir L,Gill AC,van Heyningen V,Davey MG,,FitzPatrick DR

Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.
Neuron92(5)1020-1035(2016 Dec)
Wu XS,Lee SH,Sheng J,Zhang Z,Zhao WD,Wang D,Jin Y,Charnay P,Ervasti JM,Wu LG

Widespread macromolecular interaction perturbations in human genetic disorders.
Cell161(3)647-660(2015 Apr)
Sahni N,Yi S,Taipale M,Fuxman Bass JI,Coulombe-Huntington J,Yang F,Peng J,Weile J,Karras GI,Wang Y,Kov?cs IA,Kamburov A,Krykbaeva I,Lam MH,Tucker G,Khurana V,Sharma A,Liu YY,Yachie N,Zhong Q,Shen Y,Palagi A,San-Miguel A,Fan C,Balcha D,Dricot A,Jordan DM,Walsh JM,Shah AA,Yang X,Stoyanova AK,Leighton A,Calderwood MA,Jacob Y,Cusick ME,Salehi-Ashtiani K,Whitesell LJ,Sunyaev S,Berger B,Barab?si AL,Charloteaux B,Hill DE,Hao T,Roth FP,Xia Y,Walhout AJM,Lindquist S,Vidal M

Possible role of non-muscle alpha-actinins in muscle cell mechanosensitivity.
PloS one9(4)e96395(2014)
Ogneva IV,Biryukov NS,Leinsoo TA,Larina IM

SILAC-based proteomics of human primary endothelial cell morphogenesis unveils tumor angiogenic markers.
Molecular & cellular proteomics : MCP12(12)3599-611(2013 Dec)
Zanivan S,Maione F,Hein MY,Hern?ndez-Fernaud JR,Ostasiewicz P,Giraudo E,Mann M

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Actg1 cDNA ORF clone, Rattus norvegicus(Norway rat)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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