Actg1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Actg1
Entrez Gene ID	11465
Full Name	actin, gamma, cytoplasmic 1
Synonyms	AL023024,Actg,Actl,E51
General protein information	Preferred Names actin, gamma, cytoplasmic 1 Names actin, cytoplasmic 2 beta-actin FE-3 gamma-actin
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 11 Map Location: 11\|11 E2
Summary	Actins are highly conserved proteins that are involved in various types of cell motility and maintenance of the cytoskeleton. In vertebrates, three main groups of actin isoforms, alpha, beta, and gamma, have been identified. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton, and as mediators of internal cell motility. Actin, gamma 1, encoded by this gene, is a cytoplasmic actin found in non-muscle cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015].

mRNA and Protein(s)

mRNA	Protein	Name
NM_009609.2	NP_033739.1	actin, cytoplasmic 2
NM_001313923.1	NP_001300852.1	actin, cytoplasmic 2 isoform 2
NM_009609.3	NP_033739.1	actin, cytoplasmic 2 isoform 1

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	act-1	NP_505819.1
Schizosaccharomyces pombe (fission yeast)	act1	NP_595618.1
Gallus gallus (chicken)	ACTG1	NP_001007825.1
Anopheles gambiae (African malaria mosquito)	ACT5C_ANOGA	XP_003437050.1
Eremothecium gossypii	AGOS_ABR222W	NP_983171.1
Arabidopsis thaliana (thale cress)	ACT8	NP_175350.1
Oryza sativa (rice)	Os03g0718100	NP_001051086.1
Mus musculus (house mouse)	Actg1	NP_033739.1
Rattus norvegicus (Norway rat)	Actg1	NP_001120921.1
Danio rerio (zebrafish)	actb1	NP_571106.1
Caenorhabditis elegans (roundworm)	act-3	NP_505817.1
Caenorhabditis elegans (roundworm)	act-2	NP_505818.1
Kluyveromyces lactis	KLLA0D05357g	XP_453299.1
Magnaporthe oryzae (rice blast fungus)	MGG_03982	XP_003719871.1
Neurospora crassa	NCU04173	XP_961133.2
Homo sapiens (human)	ACTG1	NP_001605.1
Bos taurus (cattle)	ACTG1	NP_001028790.1
Danio rerio (zebrafish)	actb2	NP_853632.3
Drosophila melanogaster (fruit fly)	Act5C	NP_001014726.1
Saccharomyces cerevisiae (baker's yeast)	ACT1	NP_116614.1
	ACTG1	XP_001110892.2
Caenorhabditis elegans (roundworm)	act-4	NP_508841.1
Arabidopsis thaliana (thale cress)	ACT2	NP_188508.1
Xenopus tropicalis (tropical clawed frog)	actg1	NP_989332.1

Gene Ontology
Bibliography

Related articles in PubMed

Impaired muscle relaxation and mitochondrial fission associated with genetic ablation of cytoplasmic actin isoforms.
O'Rourke AR, Lindsay A, Tarpey MD, Yuen S, McCourt P, Nelson DM, Perrin BJ, Thomas DD, Spangenburg EE, Lowe DA, Ervasti JM
The FEBS journal(2017 Dec)

Global Hypertranscription in the Mouse Embryonic Germline.
Percharde M, Wong P, Ramalho-Santos M
Cell reports19(10)1987-1996(2017 Jun)

Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.
Wu XS, Lee SH, Sheng J, Zhang Z, Zhao WD, Wang D, Jin Y, Charnay P, Ervasti JM, Wu LG
Neuron92(5)1020-1035(2016 Dec)

Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic ?-actin levels.
Schreiber J, V?gh MJ, Dawitz J, Kroon T, Loos M, Labont? D, Li KW, Van Nierop P, Van Diepen MT, De Zeeuw CI, Kneussel M, Meredith RM, Smit AB, Van Kesteren RE
The Journal of cell biology211(3)569-86(2015 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Two actin isoforms, beta- and gamma-actin, are crucial for slow, rapid, bulk, and overshoot endocytosis at large calyx-type synapses, and for slow endocytosis and bulk endocytosis at small hippocampal synapses.
Title: Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.

Splicing to include exon 3a is concomitant with previously described down-regulation of Actg1 in differentiating C2C12 cells
Title: A novel actin mRNA splice variant regulates ACTG1 expression.

These data support an essential role for beta-actin in regulating cell migration and gene expression through control of the cellular G-actin pool
Title: ?-Actin specifically controls cell growth, migration, and the G-actin pool.

Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.
Title: Beta- and gamma-cytoplasmic actins are required for meiosis in mouse oocytes.

Results demonstrate overlapping developmental roles but unique post-developmental functions for beta-actin and gamma-actin in maintaining hair cell stereocilia.
Title: ?-actin and ?-actin are each dispensable for auditory hair cell development but required for Stereocilia maintenance.

The following Actg1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Actg1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu67055	NM_001313923.1 Latest version!	Mus musculus actin, gamma, cytoplasmic 1 (Actg1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$139.30 $199.00
OMu14638	NM_009609.3 Latest version!	Mus musculus actin, gamma, cytoplasmic 1 (Actg1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
View Old Accession Versions >>
OMu14638	NM_009609.2	Mus musculus actin, gamma, cytoplasmic 1 (Actg1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$223.30 $319.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Actg1 ( NM_001313923.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001300852.1 Mus musculus actin, gamma, cytoplasmic 1 (Actg1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OMu67055
Clone ID Related Accession (Same CDS sequence)	NM_001313923.1
Accession Version	NM_001313923.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 762bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Mus musculus(house mouse)
Product	actin, cytoplasmic 2 isoform 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK153446.1, BU706992.1 and CX567990.1. On Sep 11, 2015 this sequence version replaced XM_011248670.1. Transcript Variant: This variant (2) contains an alternate exon, which causes translation initiation at a downstream AUG compared to variant 1. The resulting isoform (2) is shorter at the N-terminus compared to isoform 1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## CDS exon combination :: BC018571.1, BF580010.1 [ECO:0000331] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849375 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGTTTGAAA CCTTCAATAC CCCAGCCATG TACGTGGCCA TTCAGGCGGT GCTGTCCTTG 
TATGCATCTG GGCGCACCAC TGGCATTGTC ATGGACTCTG GTGACGGGGT CACACACACA 
GTGCCCATCT ATGAGGGCTA CGCCCTTCCC CACGCCATCT TGCGTCTGGA CCTGGCTGGC 
CGGGACCTGA CAGACTACCT CATGAAGATC CTGACTGAAC GGGGCTACAG CTTTACCACC 
ACTGCTGAGA GGGAAATTGT TCGTGACATA AAGGAGAAGC TGTGCTATGT TGCCCTGGAT 
TTTGAGCAAG AAATGGCTAC TGCTGCATCA TCTTCCTCCT TGGAGAAGAG TTACGAGCTG 
CCCGACGGGC AGGTGATCAC CATTGGCAAT GAGCGGTTCC GGTGTCCGGA GGCACTCTTC 
CAGCCTTCCT TCCTGGGCAT GGAGTCCTGT GGTATCCATG AGACCACTTT CAACTCCATC 
ATGAAGTGTG ATGTGGATAT CCGCAAAGAC CTGTATGCCA ATACAGTGCT GTCTGGTGGT 
ACCACCATGT ACCCAGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTAGCACCT 
AGCACGATGA AGATTAAGAT CATTGCTCCC CCTGAGCGCA AGTACTCAGT CTGGATCGGT 
GGCTCCATTC TGGCCTCACT GTCCACCTTC CAGCAGATGT GGATCAGCAA GCAGGAGTAT 
GATGAGTCAG GCCCCTCCAT CGTCCACCGC AAATGCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001300852.1
CDS	489..1250
Translation	MFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001313923.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin, gamma, cytoplasmic 1 (Actg1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001313923.1

ORF Insert Sequence:

ATGTTTGAAA CCTTCAATAC CCCAGCCATG TACGTGGCCA TTCAGGCGGT GCTGTCCTTG 
TATGCATCTG GGCGCACCAC TGGCATTGTC ATGGACTCTG GTGACGGGGT CACACACACA 
GTGCCCATCT ATGAGGGCTA CGCCCTTCCC CACGCCATCT TGCGTCTGGA CCTGGCTGGC 
CGGGACCTGA CAGACTACCT CATGAAGATC CTGACTGAAC GGGGCTACAG CTTTACCACC 
ACTGCTGAGA GGGAAATTGT TCGTGACATA AAGGAGAAGC TGTGCTATGT TGCCCTGGAT 
TTTGAGCAAG AAATGGCTAC TGCTGCATCA TCTTCCTCCT TGGAGAAGAG TTACGAGCTG 
CCCGACGGGC AGGTGATCAC CATTGGCAAT GAGCGGTTCC GGTGTCCGGA GGCACTCTTC 
CAGCCTTCCT TCCTGGGCAT GGAGTCCTGT GGTATCCATG AGACCACTTT CAACTCCATC 
ATGAAGTGTG ATGTGGATAT CCGCAAAGAC CTGTATGCCA ATACAGTGCT GTCTGGTGGT 
ACCACCATGT ACCCAGGCAT TGCTGACAGG ATGCAGAAGG AGATCACAGC CCTAGCACCT 
AGCACGATGA AGATTAAGAT CATTGCTCCC CCTGAGCGCA AGTACTCAGT CTGGATCGGT 
GGCTCCATTC TGGCCTCACT GTCCACCTTC CAGCAGATGT GGATCAGCAA GCAGGAGTAT 
GATGAGTCAG GCCCCTCCAT CGTCCACCGC AAATGCTTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actg1 ( NM_009609.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033739.1 Mus musculus actin, gamma, cytoplasmic 1 (Actg1), mRNA.

Price & Availability↑

CloneID	OMu14638
Clone ID Related Accession (Same CDS sequence)	NM_009609.2 , NM_009609.3
Accession Version	NM_009609.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2015-02-14
Organism	Mus musculus(House mouse)
Product	actin, cytoplasmic 2
Comment

ATGGAAGAAG AAATCGCCGC ACTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCCGGC 
TTTGCTGGCG ACGACGCCCC CAGGGCCGTG TTCCCTTCCA TCGTAGGGCG CCCCCGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAA GACTCATACG TGGGTGACGA GGCCCAGAGC 
AAGAGGGGTA TCCTGACCCT GAAGTACCCT ATCGAACACG GCATTGTCAC TAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTG 
TCCTTGTATG CATCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAACGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCCTTGGA GAAGAGTTAC 
GAGCTGCCCG ACGGGCAGGT GATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGTA TCCATGAGAC CACTTTCAAC 
TCCATCATGA AGTGTGATGT GGATATCCGC AAAGACCTGT ATGCCAATAC AGTGCTGTCT 
GGTGGTACCA CCATGTACCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTA 
GCACCTAGCA CGATGAAGAT TAAGATCATT GCTCCCCCTG AGCGCAAGTA CTCAGTCTGG 
ATCGGTGGCT CCATTCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGATG AGTCAGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033739.1
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_009609.2
Organism	Mus musculus(House mouse)
Definition	Mus musculus actin, gamma, cytoplasmic 1 (Actg1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009609.2

ORF Insert Sequence:

ATGGAAGAAG AAATCGCCGC ACTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCCGGC 
TTTGCTGGCG ACGACGCCCC CAGGGCCGTG TTCCCTTCCA TCGTAGGGCG CCCCCGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAA GACTCATACG TGGGTGACGA GGCCCAGAGC 
AAGAGGGGTA TCCTGACCCT GAAGTACCCT ATCGAACACG GCATTGTCAC TAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTG 
TCCTTGTATG CATCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAACGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCCTTGGA GAAGAGTTAC 
GAGCTGCCCG ACGGGCAGGT GATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGTA TCCATGAGAC CACTTTCAAC 
TCCATCATGA AGTGTGATGT GGATATCCGC AAAGACCTGT ATGCCAATAC AGTGCTGTCT 
GGTGGTACCA CCATGTACCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTA 
GCACCTAGCA CGATGAAGAT TAAGATCATT GCTCCCCCTG AGCGCAAGTA CTCAGTCTGG 
ATCGGTGGCT CCATTCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGATG AGTCAGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Actg1 ( NM_009609.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_033739.1 Mus musculus actin, gamma, cytoplasmic 1 (Actg1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OMu14638
Clone ID Related Accession (Same CDS sequence)	NM_009609.2 , NM_009609.3
Accession Version	NM_009609.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-30
Organism	Mus musculus(house mouse)
Product	actin, cytoplasmic 2 isoform 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AK153446.1 and CX567990.1. On Sep 11, 2015 this sequence version replaced NM_009609.2. Transcript Variant: This variant (1) encodes the longer isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AK153446.1, AF195094.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN01164141 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGAAGAAG AAATCGCCGC ACTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCCGGC 
TTTGCTGGCG ACGACGCCCC CAGGGCCGTG TTCCCTTCCA TCGTAGGGCG CCCCCGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAA GACTCATACG TGGGTGACGA GGCCCAGAGC 
AAGAGGGGTA TCCTGACCCT GAAGTACCCT ATCGAACACG GCATTGTCAC TAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTG 
TCCTTGTATG CATCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAACGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCCTTGGA GAAGAGTTAC 
GAGCTGCCCG ACGGGCAGGT GATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGTA TCCATGAGAC CACTTTCAAC 
TCCATCATGA AGTGTGATGT GGATATCCGC AAAGACCTGT ATGCCAATAC AGTGCTGTCT 
GGTGGTACCA CCATGTACCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTA 
GCACCTAGCA CGATGAAGAT TAAGATCATT GCTCCCCCTG AGCGCAAGTA CTCAGTCTGG 
ATCGGTGGCT CCATTCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGATG AGTCAGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_033739.1
CDS	78..1205
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_009609.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus actin, gamma, cytoplasmic 1 (Actg1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_009609.3

ORF Insert Sequence:

ATGGAAGAAG AAATCGCCGC ACTCGTCATT GACAATGGCT CCGGCATGTG CAAAGCCGGC 
TTTGCTGGCG ACGACGCCCC CAGGGCCGTG TTCCCTTCCA TCGTAGGGCG CCCCCGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAA GACTCATACG TGGGTGACGA GGCCCAGAGC 
AAGAGGGGTA TCCTGACCCT GAAGTACCCT ATCGAACACG GCATTGTCAC TAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAATGAGC TGCGTGTGGC TCCTGAGGAG 
CACCCGGTGC TTCTGACCGA GGCCCCCCTG AACCCCAAAG CTAACAGAGA GAAGATGACG 
CAGATAATGT TTGAAACCTT CAATACCCCA GCCATGTACG TGGCCATTCA GGCGGTGCTG 
TCCTTGTATG CATCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGTGA CGGGGTCACA 
CACACAGTGC CCATCTATGA GGGCTACGCC CTTCCCCACG CCATCTTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACAGA CTACCTCATG AAGATCCTGA CTGAACGGGG CTACAGCTTT 
ACCACCACTG CTGAGAGGGA AATTGTTCGT GACATAAAGG AGAAGCTGTG CTATGTTGCC 
CTGGATTTTG AGCAAGAAAT GGCTACTGCT GCATCATCTT CCTCCTTGGA GAAGAGTTAC 
GAGCTGCCCG ACGGGCAGGT GATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCA 
CTCTTCCAGC CTTCCTTCCT GGGCATGGAG TCCTGTGGTA TCCATGAGAC CACTTTCAAC 
TCCATCATGA AGTGTGATGT GGATATCCGC AAAGACCTGT ATGCCAATAC AGTGCTGTCT 
GGTGGTACCA CCATGTACCC AGGCATTGCT GACAGGATGC AGAAGGAGAT CACAGCCCTA 
GCACCTAGCA CGATGAAGAT TAAGATCATT GCTCCCCCTG AGCGCAAGTA CTCAGTCTGG 
ATCGGTGGCT CCATTCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT CAGCAAGCAG 
GAGTATGATG AGTCAGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Impaired muscle relaxation and mitochondrial fission associated with genetic ablation of cytoplasmic actin isoforms.
The FEBS journal(2017 Dec)
O'Rourke AR,Lindsay A,Tarpey MD,Yuen S,McCourt P,Nelson DM,Perrin BJ,Thomas DD,Spangenburg EE,Lowe DA,Ervasti JM

Global Hypertranscription in the Mouse Embryonic Germline.
Cell reports19(10)1987-1996(2017 Jun)
Percharde M,Wong P,Ramalho-Santos M

Actin Is Crucial for All Kinetically Distinguishable Forms of Endocytosis at Synapses.
Neuron92(5)1020-1035(2016 Dec)
Wu XS,Lee SH,Sheng J,Zhang Z,Zhao WD,Wang D,Jin Y,Charnay P,Ervasti JM,Wu LG

Ubiquitin ligase TRIM3 controls hippocampal plasticity and learning by regulating synaptic ?-actin levels.
The Journal of cell biology211(3)569-86(2015 Nov)
Schreiber J,V?gh MJ,Dawitz J,Kroon T,Loos M,Labont? D,Li KW,Van Nierop P,Van Diepen MT,De Zeeuw CI,Kneussel M,Meredith RM,Smit AB,Van Kesteren RE

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Actg1 cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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