ACTG1 cDNA ORF clone, Homo sapiens(human)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACTG1
Entrez Gene ID	71
Full Name	actin gamma 1
Synonyms	ACT,ACTG,DFNA20,DFNA26,HEL-176
General protein information	Preferred Names actin gamma 1 Names actin, cytoplasmic 2 cytoskeletal gamma-actin epididymis luminal protein 176
Gene Type	protein-coding
Organism	Homo sapiens(human)
Genome	Chromosome: 17 Map Location: 17q25.3
Summary	Actins are highly conserved proteins that are involved in various types of cell motility and in maintenance of the cytoskeleton. Three main groups of actin isoforms have been identified in vertebrate animals: alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. Actin gamma 1, encoded by this gene, is a cytoplasmic actin found in all cell types. Mutations in this gene are associated with DFNA20/26, a subtype of autosomal dominant non-syndromic sensorineural progressive hearing loss and also with Baraitser-Winter syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2017].
Disorder MIM:	102560
Disorder Html:	Deafness, autosomal dominant 20/26, 604717 (3); Baraitser-Winter syndrome 2, 614583 (3)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001614.3	NP_001605.1	actin, cytoplasmic 2
NM_001614.5	NP_001605.1	actin, cytoplasmic 2
NM_001199954.1	NP_001186883.1	actin, cytoplasmic 2
NM_001199954.2	NP_001186883.1	actin, cytoplasmic 2

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	act-1	NP_505819.1
Schizosaccharomyces pombe (fission yeast)	act1	NP_595618.1
Gallus gallus (chicken)	ACTG1	NP_001007825.1
Anopheles gambiae (African malaria mosquito)	ACT5C_ANOGA	XP_003437050.1
Eremothecium gossypii	AGOS_ABR222W	NP_983171.1
Arabidopsis thaliana (thale cress)	ACT8	NP_175350.1
Oryza sativa (rice)	Os03g0718100	NP_001051086.1
Mus musculus (house mouse)	Actg1	NP_033739.1
Rattus norvegicus (Norway rat)	Actg1	NP_001120921.1
Danio rerio (zebrafish)	actb1	NP_571106.1
Caenorhabditis elegans (roundworm)	act-3	NP_505817.1
Caenorhabditis elegans (roundworm)	act-2	NP_505818.1
Kluyveromyces lactis	KLLA0D05357g	XP_453299.1
Magnaporthe oryzae (rice blast fungus)	MGG_03982	XP_003719871.1
Neurospora crassa	NCU04173	XP_961133.2
Homo sapiens (human)	ACTG1	NP_001605.1
Bos taurus (cattle)	ACTG1	NP_001028790.1
Danio rerio (zebrafish)	actb2	NP_853632.3
Drosophila melanogaster (fruit fly)	Act5C	NP_001014726.1
Saccharomyces cerevisiae (baker's yeast)	ACT1	NP_116614.1
	ACTG1	XP_001110892.2
Caenorhabditis elegans (roundworm)	act-4	NP_508841.1
Arabidopsis thaliana (thale cress)	ACT2	NP_188508.1
Xenopus tropicalis (tropical clawed frog)	actg1	NP_989332.1

Gene Ontology
Bibliography

Related articles in PubMed

Actin Gamma 1, a new skin cancer pathogenic gene, identified by the biological feature-based classification.
Dong X, Han Y, Sun Z, Xu J
Journal of cellular biochemistry119(2)1406-1419(2018 Feb)

A novel de novo mutation of ACTG1 in two sporadic non-syndromic hearing loss cases.
Wang H, Guan J, Lan L, Yu L, Xie L, Liu X, Yang J, Zhao C, Wang D, Wang Q
Science China. Life sciences(2018 Jan)

A recurrent de novo mutation in ACTG1 causes isolated ocular coloboma.
Rainger J, Williamson KA, Soares DC, Truch J, Kurian D, Gillessen-Kaesbach G, Seawright A, Prendergast J, Halachev M, Wheeler A, McTeir L, Gill AC, van Heyningen V, Davey MG, , FitzPatrick DR
Human mutation38(8)942-946(2017 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We have identified a three-generation pedigree segregating a novel mutation in the ACTG1 gene that causes Baraitser-Winter Syndrome with extremely variable expressivity, leading to an initial diagnosis of isolated AD hearing loss in two members.
Title: A novel mutation in ACTG1 causing Baraitser-Winter syndrome with extremely variable expressivity in three generations.

we expand the clinical spectrum of the ACTG1-associated Baraitser-Winter cerebrofrontofacial syndrome demonstrating the mild end of the facial and brain manifestations.
Title: Update on the ACTG1-associated Baraitser-Winter cerebrofrontofacial syndrome.

a novel mutation in ACTG1 was found to be co-segregated with hearing loss and the genetic cause of autosomal dominant nonsyndromic hearing impairment in a Chinese family
Title: Phenotypic Heterogeneity in a DFNA20/26 family segregating a novel ACTG1 mutation.

Data indicate that F-actin is significantly elevated in septic shock, and F-actin and the F:G-actin ratio are potential biomarkers for the diagnosis of septic shock.
Title: Plasma levels of F-actin and F:G-actin ratio as potential new biomarkers in patients with septic shock.

Three unrelated cases of rare ACTG1 variants in fetal microlissencephaly have been described.
Title: Rare ACTG1 variants in fetal microlissencephaly.

The following ACTG1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACTG1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OHu14728	NM_001614.5 Latest version!	Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu14728	NM_001199954.2 Latest version!	Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
View Old Accession Versions >>
OHu14728	NM_001614.3	Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
OHu14728	NM_001199954.1	Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	in pcDNA3.1-C-(k)DYK	$159.50-$223.30 $319.00
Hide Old Accession Versions >>

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***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACTG1 ( NM_001614.3 ) cDNA ORF clone, Homo sapiens(human) -> NP_001605.1 Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14728
Clone ID Related Accession (Same CDS sequence)	NM_001614.3 , NM_001614.5 , NM_001199954.1 , NM_001199954.2
Accession Version	NM_001614.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-27
Organism	Homo sapiens(human)
Product	actin, cytoplasmic 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455954.1, X04098.1 and BC063495.1. This sequence is a reference standard in the RefSeqGene project. On Jan 4, 2011 this sequence version replaced NM_001614.2. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.34444.1, BC000292.2 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001605.1
CDS	140..1267
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001614.3
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001614.3

ORF Insert Sequence:

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTG1 ( NM_001614.5 ) cDNA ORF clone, Homo sapiens(human) -> NP_001605.1 Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.

Price & Availability↑

CloneID	OHu14728
Clone ID Related Accession (Same CDS sequence)	NM_001614.3 , NM_001614.5 , NM_001199954.1 , NM_001199954.2
Accession Version	NM_001614.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-09-25
Organism	Homo sapiens(human)
Product	actin, cytoplasmic 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from X04098.1 and BC063495.1. This sequence is a reference standard in the RefSeqGene project. On Nov 22, 2018 this sequence version replaced NM_001614.4. Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.90241.1, SRR3476690.105074.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## MANE Ensembl match :: ENST00000573283.7/ ENSP00000458435.1 RefSeq Select criteria :: based on conservation, expression, longest protein ##RefSeq-Attributes-END## COMPLETENESS: full length.

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001605.1
CDS	73..1200
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001614.5
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 1 (ACTG1), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001614.5

ORF Insert Sequence:

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTG1 ( NM_001199954.1 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186883.1 Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.

Price & Availability↑

CloneID	OHu14728
Clone ID Related Accession (Same CDS sequence)	NM_001614.3 , NM_001614.5 , NM_001199954.1 , NM_001199954.2
Accession Version	NM_001199954.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-12-26
Organism	Homo sapiens(human)
Product	actin, cytoplasmic 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from DB455954.1, AC139149.6 and BC063495.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.332646.1, SRR1163655.310684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186883.1
CDS	259..1386
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001199954.1
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199954.1

ORF Insert Sequence:

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACTG1 ( NM_001199954.2 ) cDNA ORF clone, Homo sapiens(human) -> NP_001186883.1 Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.

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CloneID	OHu14728
Clone ID Related Accession (Same CDS sequence)	NM_001614.3 , NM_001614.5 , NM_001199954.1 , NM_001199954.2
Accession Version	NM_001199954.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1128bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-16
Organism	Homo sapiens(human)
Product	actin, cytoplasmic 2
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from AC139149.6 and BC063495.1. On Feb 17, 2018 this sequence version replaced NM_001199954.1. Transcript Variant: This variant (1) represents the longest transcript. Variants 1 and 2 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: SRR1163658.332646.1, SRR1163655.310684.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA1965299, SAMEA1966682 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: full length.

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001186883.1
CDS	192..1319
Translation	MEEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVMDSGDGVTHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTAEREIVRDIKEKLCYVALDFEQEMATAASSSSLEKSYELPDGQVITIGNERFRCPEALFQPSFLGMESCGIHETTFNSIMKCDVDIRKDLYANTVLSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWISKQEYDESGPSIVHRKCF

Target ORF information:

RefSeq Version	NM_001199954.2
Organism	Homo sapiens(human)
Definition	Homo sapiens actin gamma 1 (ACTG1), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001199954.2

ORF Insert Sequence:

ATGGAAGAAG AGATCGCCGC GCTGGTCATT GACAATGGCT CCGGCATGTG CAAAGCTGGT 
TTTGCTGGGG ACGACGCTCC CCGAGCCGTG TTTCCTTCCA TCGTCGGGCG CCCCAGACAC 
CAGGGCGTCA TGGTGGGCAT GGGCCAGAAG GACTCCTACG TGGGCGACGA GGCCCAGAGC 
AAGCGTGGCA TCCTGACCCT GAAGTACCCC ATTGAGCATG GCATCGTCAC CAACTGGGAC 
GACATGGAGA AGATCTGGCA CCACACCTTC TACAACGAGC TGCGCGTGGC CCCGGAGGAG 
CACCCAGTGC TGCTGACCGA GGCCCCCCTG AACCCCAAGG CCAACAGAGA GAAGATGACT 
CAGATTATGT TTGAGACCTT CAACACCCCG GCCATGTACG TGGCCATCCA GGCCGTGCTG 
TCCCTCTACG CCTCTGGGCG CACCACTGGC ATTGTCATGG ACTCTGGAGA CGGGGTCACC 
CACACGGTGC CCATCTACGA GGGCTACGCC CTCCCCCACG CCATCCTGCG TCTGGACCTG 
GCTGGCCGGG ACCTGACCGA CTACCTCATG AAGATCCTCA CTGAGCGAGG CTACAGCTTC 
ACCACCACGG CCGAGCGGGA AATCGTGCGC GACATCAAGG AGAAGCTGTG CTACGTCGCC 
CTGGACTTCG AGCAGGAGAT GGCCACCGCC GCATCCTCCT CTTCTCTGGA GAAGAGCTAC 
GAGCTGCCCG ATGGCCAGGT CATCACCATT GGCAATGAGC GGTTCCGGTG TCCGGAGGCG 
CTGTTCCAGC CTTCCTTCCT GGGTATGGAA TCTTGCGGCA TCCACGAGAC CACCTTCAAC 
TCCATCATGA AGTGTGACGT GGACATCCGC AAAGACCTGT ACGCCAACAC GGTGCTGTCG 
GGCGGCACCA CCATGTACCC GGGCATTGCC GACAGGATGC AGAAGGAGAT CACCGCCCTG 
GCGCCCAGCA CCATGAAGAT CAAGATCATC GCACCCCCAG AGCGCAAGTA CTCGGTGTGG 
ATCGGTGGCT CCATCCTGGC CTCACTGTCC ACCTTCCAGC AGATGTGGAT TAGCAAGCAG 
GAGTACGACG AGTCGGGCCC CTCCATCGTC CACCGCAAAT GCTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Actin Gamma 1, a new skin cancer pathogenic gene, identified by the biological feature-based classification.
Journal of cellular biochemistry119(2)1406-1419(2018 Feb)
Dong X,Han Y,Sun Z,Xu J

A novel de novo mutation of ACTG1 in two sporadic non-syndromic hearing loss cases.
Science China. Life sciences(2018 Jan)
Wang H,Guan J,Lan L,Yu L,Xie L,Liu X,Yang J,Zhao C,Wang D,Wang Q

A recurrent de novo mutation in ACTG1 causes isolated ocular coloboma.
Human mutation38(8)942-946(2017 Aug)
Rainger J,Williamson KA,Soares DC,Truch J,Kurian D,Gillessen-Kaesbach G,Seawright A,Prendergast J,Halachev M,Wheeler A,McTeir L,Gill AC,van Heyningen V,Davey MG,,FitzPatrick DR

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ACTG1 cDNA ORF clone, Homo sapiens(human)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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