myp2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	myp2
Entrez Gene ID	2543576
Full Name	myosin II heavy chain Myo3
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001019246.2	NP_593816.1	Myo3-like myosin II heavy chain myp2

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	MYH9	NP_001104237.1
Danio rerio (zebrafish)	myh9a	NP_001091647.2
Xenopus tropicalis (tropical clawed frog)	myh9	XP_002934764.2
Bos taurus (cattle)	MYH9	NP_001179691.1
Mus musculus (house mouse)	Myh9	NP_071855.2
Gallus gallus (chicken)	MYH9	NP_990808.1
Schizosaccharomyces pombe (fission yeast)	myp2	NP_593816.1
Pan troglodytes (chimpanzee)	MYH9	XP_003953928.1
Magnaporthe oryzae (rice blast fungus)	MGG_03060	XP_003720621.1
Neurospora crassa	NCU00551	XP_964712.2
Homo sapiens (human)	MYH9	NP_002464.1
Rattus norvegicus (Norway rat)	LOC100911597	XP_003750426.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

We provide evidence that the myosin II protein Myp2 and the myosin V protein Myo51 play roles in recruiting TORC2 to the CAR.We go on to show that TORC2-dependent phosphorylation of actin-capping protein 1 (Acp1, a known regulator of cytokinesis) controls CAR stability, modulates Acp1-Acp2 heterodimer formation and is essential for survival upon stress.
Title: TOR complex 2 localises to the cytokinetic actomyosin ring and controls the fidelity of cytokinesis.

Three myosins function in a hierarchal, complementary manner to accomplish cytokinesis, with Myo2 and Myo51 taking the lead during contractile ring assembly and Myp2 making the greatest contribution to constriction.
Title: Three myosins contribute uniquely to the assembly and constriction of the fission yeast cytokinetic contractile ring.

The following myp2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the myp2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102415	NM_001019246.2 Latest version!	Schizosaccharomyces pombe Myo3-like myosin II heavy chain myp2 (myp2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

myp2 ( NM_001019246.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593816.1 Schizosaccharomyces pombe Myo3-like myosin II heavy chain myp2 (myp2), mRNA.

Price & Availability↑

CloneID	OSc102415
Clone ID Related Accession (Same CDS sequence)	NM_001019246.2
Accession Version	NM_001019246.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6315bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Myo3-like myosin II heavy chain myp2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001019246.1.

ATGTCGTATC TTTCAAAAAA TGGCTCTAAC GACAACAATA ATATTATCAA AAAGCTAGTA 
GATGCTGAAA AACATTGCAA TGCCGTGAAA GACGCGTCTT TTGATGAACG GACATGGATC 
TGGATTCCTG ATTCGAAGGA ATCATTCGTG AAAGCTTGGA TAGTTGAAGA TTTAGGAGAA 
AAATATAGGG TGAAATTAGA AAGAGATGGC TCGGAAAGAA TTGTGGATGG ATTTGATGCT 
GAAAAAGTAA ATCCACCGAA GTTTGATATG GTTGATGACA TGGCTGCGTT GACATGTCTC 
AACGAACCAT CAGTAGTTAA CAACCTTACT CAGCGTTATG AGAAAGATTT AATATATACC 
TATTCAGGCT TGTTTCTCGT TGCTGTAAAC CCCTATTGTC ATTTACCAAT TTATGGCGAT 
GATGTTGTGC GAAAATACCA ATCTAAACAG TTTAAAGAAA CTAAACCCCA TATTTTTGGA 
ACAGCTGATG CCGCATATCG AAGTTTATTA GAAAGGAGGA TCAATCAATC TATTTTAGTT 
ACTGGTGAAT CAGGAGCTGG AAAAACTGAA ACTACTAAAA AGGTTATTCA GTACCTAACA 
TCGGTCACTG ATGCTTCCAC AAGCGATTCT CAACAGTTGG AGAAGAAAAT CCTTGAAACG 
AATCCTGTGT TGGAGGCTTT TGGGAATGCT CAGACTGTCC GGAATAACAA CTCATCCCGC 
TTTGGCAAGT TCATACGAAT TGAATTTTCT AATAATGGAA GCATCGTTGG AGCGAATTTA 
GACTGGTACC TTTTGGAAAA ATCTCGCGTT ATACATCCTT CCTCCAATGA ACGCAATTAT 
CACGTCTTTT ATCAACTACT TCGAGGTGCT GATGGATCAT TGCTTGAAAG TCTTTTCCTT 
GATCGATATG TTGACCATTA CTCATATTTA AAAAATGGTC TCAAGCATAT TAATGGAGTC 
GATGATGGCA AAGAATTTCA AAAATTATGC TTTGGTCTCA GAACTTTGGG GTTTGATAAT 
AACGAAATTC ACAGTTTGTT CTTAATTATA GCTTCAATTC TTCATATAGG AAATATTGAA 
GTAGCATCTG ATCGTTCGGG GCAAGCTAGA TTTCCTTCCT TAACGCAAAT TGATCAATTA 
TGCCATTTAC TCGAAATCCC TGTTGATGGA TTTGTTAATG CAGCTTTACA CCCAAAGTCC 
AAAGCTGGAC GAGAATGGAT TGTGACGGCA AGAACACGTG AACAAGTAGT CCATACACTA 
CAATCCTTAG CCAAAGGCCT TTATGAGCGT AATTTTGCGC ATTTAGTGAA ACGTCTGAAT 
CAGACTATGT ATTATTCTCA GTCTGAACAT GATGGGTTCA TTGGGGTTTT AGATATTGCT 
GGGTTTGAAA TTTTCACTTT TAATAGTTTT GAGCAACTCT GTATTAATTT TACAAATGAA 
AAGCTTCAGC AATTCTTTAA TCATTACATG TTCGTTCTGG AACAAGAAGA ATATACACAA 
GAACGCATCG AATGGGATTT TATTGACTAC GGAAATGATT TGCAGCCCAC CATAGATGCT 
ATCGAAAAAT CTGAGCCCAT TGGTATTTTT TCTTGCCTCG ACGAGGATTG TGTTATGCCA 
ATGGCTACTG ATGCTACATT CACAGAAAAA CTTCACCTTC TTTTTAAGGG TAAATCCGAT 
ATATACCGTC CTAAAAAGTT TTCTAGTGAA GGCTTTGTTT TAAAACATTA TGCTGGTGAT 
GTCGAATATG ATACCAAAGA TTGGCTGGAG AAAAACAAAG ATCCTCTAAA TGCATGTCTT 
GCCGCACTAA TGTTCAAATC TACTAACAGT CATGTGTCTT CTCTTTTTGA TGATTATTCG 
TCAAATGCTA GTGGTCGAGA TAACATTGAA AAGAAAGGAA TCTTTCGTAC TGTGTCGCAG 
CGTCATAGGA GACAGCTTTC TAGTCTTATG CACCAACTCG AAGCAACTCA GCCTCACTTT 
GTCAGATGCA TTATTCCAAA CAATTTGAAG CAGCCTCACA ATTTGGATAA ATCTCTCGTT 
CTTCATCAAC TTCGATGCAA CGGTGTACTG GAGGGTATTA GAATTGCTCA AACTGGATTT 
CCAAACAAAC TATTTTATAC AGAATTTAGA GCTCGTTATG GGATTCTTTC GCAGTCATTA 
AAGAGAGGAT ATGTCGAAGC AAAAAAGGCA ACAATAACGA TCATCAATGA ATTAAAATTA 
CCTAGTACCG TATACCGTTT GGGTGAAACA AAGGTATTTT TTAAAGCCAG TGTCTTGGGT 
TCTTTAGAGG ATCGCAGAAA CGCTTTACTG CGGGTGATAT TTAATTCATT TTCAGCTCGC 
ATTCGTGGGT TTTTGACAAG ACGGAGACTT TACCGGTTTA ATCATAGGCA AGATGCAGCT 
ATCCTTCTAC AGCATAACTT GAGGCAGTTG AAGTTATTGA AGCCACATCC TTGGTGGAAT 
CTTTTTCTGC ATCTTAAACC GTTATTGGGA ACAACCCAGA CTGATGAGTA TTTAAGAAGA 
AAAGACGCAC TCATAAATAA TTTACAGAAT CAATTGGAAA GTACCAAGGA AGTTGCAAAC 
GAATTAACTA TTACCAAAGA ACGAGTCTTA CAATTGACTA ACGATTTGCA AGAGGAACAA 
GCTCTGGCAC ATGAAAAGGA CATTTTGGTA GAAAGAGCCA ATAGCCGGGT TGAAGTTGTT 
CATGAGAGAC TGTCAAGTTT GGAAAATCAA GTAACTATTG CTGATGAAAA ATATGAGTTT 
TTGTATGCAG AGAAGCAAAG CATAGAGGAG GACCTTGCCA ACAAACAGAC AGAAATTTCT 
TATCTTTCCG ATTTATCATC TACATTGGAG AAAAAGCTGT CTTCAATTAA GAAGGACGAG 
CAAACGATTT CTTCTAAATA TAAAGAGCTT GAGAAAGACT ATCTGAATAT CATGGCGGAT 
TATCAACATA GTTCGCAGCA TTTATCAAAT CTTGAAAAGG CTATTAACGA AAAGAATTTA 
AATATTCGCG AATTGAATGA GAAACTCATG AGACTTGATG ATGAGTTACT ATTAAAGCAA 
CGAAGTTATG ATACAAAGGT TCAAGAATTA AGGGAAGAAA ACGCAAGCTT AAAAGACCAA 
TGTCGTACTT ACGAATCTCA ATTAGCAAGT CTTGTTTCCA AATACTCAGA AACAGAAAGT 
GAATTGAATA AGAAGGAAGC CGAGTTAGTT ATCTTTCAGA AGGAAATCAC GGAATACAGA 
GATCAACTTC ATAAAGCTTT TCAAAATCCT GAGAAAACTC ATAACATTAA TGATGTAAAA 
AGTGGCCCCT TAAACAGTGA TGAGAATATT TATTCTACTT CTTCGACCAC GCTTTCAATT 
TTGAAGGATG TTCAAGAGCT TAAATCATTA CATACGAAAG AAGCAAACCA GTTATCTGAA 
CGGATTAAAG AGATAAGTGA GATGCTTGAG CAAAGTATAG CGACTGAAGA AAAGCTTCGT 
AGAAAAAATT CTGAACTATG TGATATAATT GAGGCCTTAA AATACCAAAT TCAGGATCAG 
GAAACTGAAA TTATCTCTTT AAATGCTGAT AATCTGGATT TGAAAGATAC AAATGGTGTT 
TTGGAGAAGA ATGCTAGTGA TTTCATAGAC TTCCAAGGTA TAAAAAGCCG TTATGAACAT 
AAAATTTCAG ATTTACTGAA TCAGCTTCAG AAGGAAAGGT GTAAAGTTGG GCTATTAAAA 
CAAAAAACAG AAAATCGGTC AGTTACACAA CACACTTTAG ATGGAAATTC TCCACATCCA 
TCATTTGAAG AGAAACATTC AGGTGATCCT CTAAAACGCA TTGATGGTAA TAACGATGAT 
CGAAAAATTG ACAATAAATT ATTGAAAACA ATTAGTAAAA GCCTTGATGC TTTGCAACTA 
ACCGTTGAAG AAGAACTTAG CAATCTGTAT TCCCTCTCTA AGGATTTATC TTTTACTGAC 
ATTTCGGGAC ACATACCAAA CTCTATTAGG AAGCTTGAAA AAGGGTTGAG TACTTTATCA 
GAATTAAAGG AACGTCTCAA TGCATCAAAT TCTGACAGAC CTTCCCCGGA CATTTTTAAA 
GATACTCAAG CTATCATGAA TTCTCGGAAG CTATTAAGCA ATCCTAATTC TGATGCTCAA 
AGTGGTTTAA TATCATCATT GCAGAAAAAG CTTTATAATC CTGAATCTAA TATGGAATTT 
ACTGGGCTGA AACCTTTGTC TCCAAGCAAA ATCAGCAATT TACCCTCTTC TCAACCCGGC 
TCACCTTCAA AGAGATCTGG CAAAATGGAA GCACTGATTA GAAATTTTGA TCAAAATAGT 
AGTATTCCTG ATCCCTTTAT CGTTAATCAA CGGAATTCCG TGCTTCAAAC AGAGTTTGAA 
AAAATCAATT TGAAGTTAAA GGAAGCTACA AAGTCTGGAA TCTTAGATAA CAAAGATCTA 
TCAAAATTTT CGGAACTTAT ACAATCTTTG TTAAAAGAAA ATGAGGAACT TAAAAACTTA 
ACTACTAGTA ATTTAGGCAG TGATGATAAA ATGCTTGATT TTGCTCCATT ACTTGAAGAT 
GTTCCAAATA ATACTAGAAA CCAAATCAAA GGTTTTGTGG AAAAGGCAAT ATCATCGAAG 
CGAGCTATTG CAAAGTTATA TTCGGCAAGC GAAGAAAAGC TGTTTTCAAC GGAAAAAGCT 
TTAAGAGAGA TAACCAAGGA AAGAGATCGT TTGCTTCATG GGTTGCAAGG ACCTTCTGTT 
CCTACATCTC CTTTAAAGGC TCCCACTGCT TCTCAATTAA TAATTCCCAA TTTTGATGGT 
AGTATAACTA ATTATTCTGG AGAAGAAGAA ACCGAGTGGT TGCAAGAAGA AGTTAATATA 
ATGAAGATAA AGGAGCTTAC TTCTACAGTT AACAAGTACC GCGAACAGCT TGCGATGGTC 
CAGTCACTCA ATGAGCATGC AGAATCTTCA CTTTCTAAAG CTGAAAGGTC TAAAAATTAT 
CTTACCGGGC GTTTACAGGA AGTTGAAGAA CTCGCTCGCG GATTTCAAAC AACAAATGCT 
GATCTGCAAA ACGAACTGGC AGATGCTGTC GTGAAACAAA AGGAGTATGA AGTTCTTTAC 
GTTGAAAAGA GCAACGATTA TAATACTTTG CTGCTGCAAA AAGAAAAACT GATGAAACAA 
ATTGATGAGT TTCATGTCAT TCGTGTTCAA GATCTCGAGG AACGAGAAAA GAAGGATCAA 
CTACTCTTTC AACGTTATCA AAAGGAGCTA AATGGTTTCA AAGTACAGTT GGAAGAAGAA 
CGTGAGAAGA ACTTGCGCAT TAGACAAGAT AATCGGCACA TGCATGCGGA AATTGGTGAT 
ATCCGTACCA AATTTGACGA ATTAGTTTTG GAAAAGACAA ATTTGTTAAA AGAAAATTCA 
ATACTTCAAG CAGACTTACA ATCTCTTTCT CGTGTCAATA ATTCTTCCTC AACAGCGCAA 
CAAAATGCCC AAAGCCAACT TCTTTCTTTG ACTGCACAAC TCCAAGAAGT ACGAGAAGCA 
AACCAGACAT TAAGAAAAGA TCAAGATACC CTTTTAAGAG AAAATCGAAA CCTTGAGCGA 
AAACTTCACG AAGTTTCAGA GCAATTGAAC AAAAAATTCG ATAGTTCTGC AAGACCTTTT 
GATGAAATTG AAATGGAGAA AGAGGTATTA ACACTCAAGA GTAACCTTGC TCAAAAAGAT 
GACTTACTAT CATCATTAGT TGAACGCATA AAACAGATCG AAATGTTTGC GCTAAAAACT 
CAAAAAGATA GTAATAATCA TCGAGAAGAA AATCTTCAAC TGCATCGTCA ACTCGGAGTT 
TTACAAAAGG AGAAAAAGGA TTTGGAGCTC AAATTGTTTG ATTTAGATCT TAAAACTTAT 
CCCATATCAA CATCAAAGGA CGTCCGAATG CTACAAAAGC AAATATCAGA TTTGGAAGCA 
TCGTTTGCTG CTTCCGACAT CGAACGAATT AAAGGAATTG ATGAATGTCG CAATCGCGAT 
AGGACTATCC GTCAACTGGA AGCTCAAATA TCAAAATTTG ATGATGATAA AAAGAGAATA 
CAGAGCTCAG TTTCTCGTTT GGAGGAAAGA AATGCGCAAC TGAGAAATCA ATTAGAAGAT 
GTGCAAGCTT CAGAAACACA ATGGAAATTT GCACTACGTC GGACGGAACA TGCTCTTCAA 
GAAGAGCGTG AACGCGTCAA AAGTTTGGAA ACCGATTTTG ATAAATATAG ATCTTTGCTG 
GAGGGTCAAA GAGTAAAGAG AAGTGAAAGT AGATTATCCA TGAGGTCCAA TCGTTCGCCT 
AGCGTTCTAA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593816.1
CDS	32..6346
Translation	MSYLSKNGSNDNNNIIKKLVDAEKHCNAVKDASFDERTWIWIPDSKESFVKAWIVEDLGEKYRVKLERDGSERIVDGFDAEKVNPPKFDMVDDMAALTCLNEPSVVNNLTQRYEKDLIYTYSGLFLVAVNPYCHLPIYGDDVVRKYQSKQFKETKPHIFGTADAAYRSLLERRINQSILVTGESGAGKTETTKKVIQYLTSVTDASTSDSQQLEKKILETNPVLEAFGNAQTVRNNNSSRFGKFIRIEFSNNGSIVGANLDWYLLEKSRVIHPSSNERNYHVFYQLLRGADGSLLESLFLDRYVDHYSYLKNGLKHINGVDDGKEFQKLCFGLRTLGFDNNEIHSLFLIIASILHIGNIEVASDRSGQARFPSLTQIDQLCHLLEIPVDGFVNAALHPKSKAGREWIVTARTREQVVHTLQSLAKGLYERNFAHLVKRLNQTMYYSQSEHDGFIGVLDIAGFEIFTFNSFEQLCINFTNEKLQQFFNHYMFVLEQEEYTQERIEWDFIDYGNDLQPTIDAIEKSEPIGIFSCLDEDCVMPMATDATFTEKLHLLFKGKSDIYRPKKFSSEGFVLKHYAGDVEYDTKDWLEKNKDPLNACLAALMFKSTNSHVSSLFDDYSSNASGRDNIEKKGIFRTVSQRHRRQLSSLMHQLEATQPHFVRCIIPNNLKQPHNLDKSLVLHQLRCNGVLEGIRIAQTGFPNKLFYTEFRARYGILSQSLKRGYVEAKKATITIINELKLPSTVYRLGETKVFFKASVLGSLEDRRNALLRVIFNSFSARIRGFLTRRRLYRFNHRQDAAILLQHNLRQLKLLKPHPWWNLFLHLKPLLGTTQTDEYLRRKDALINNLQNQLESTKEVANELTITKERVLQLTNDLQEEQALAHEKDILVERANSRVEVVHERLSSLENQVTIADEKYEFLYAEKQSIEEDLANKQTEISYLSDLSSTLEKKLSSIKKDEQTISSKYKELEKDYLNIMADYQHSSQHLSNLEKAINEKNLNIRELNEKLMRLDDELLLKQRSYDTKVQELREENASLKDQCRTYESQLASLVSKYSETESELNKKEAELVIFQKEITEYRDQLHKAFQNPEKTHNINDVKSGPLNSDENIYSTSSTTLSILKDVQELKSLHTKEANQLSERIKEISEMLEQSIATEEKLRRKNSELCDIIEALKYQIQDQETEIISLNADNLDLKDTNGVLEKNASDFIDFQGIKSRYEHKISDLLNQLQKERCKVGLLKQKTENRSVTQHTLDGNSPHPSFEEKHSGDPLKRIDGNNDDRKIDNKLLKTISKSLDALQLTVEEELSNLYSLSKDLSFTDISGHIPNSIRKLEKGLSTLSELKERLNASNSDRPSPDIFKDTQAIMNSRKLLSNPNSDAQSGLISSLQKKLYNPESNMEFTGLKPLSPSKISNLPSSQPGSPSKRSGKMEALIRNFDQNSSIPDPFIVNQRNSVLQTEFEKINLKLKEATKSGILDNKDLSKFSELIQSLLKENEELKNLTTSNLGSDDKMLDFAPLLEDVPNNTRNQIKGFVEKAISSKRAIAKLYSASEEKLFSTEKALREITKERDRLLHGLQGPSVPTSPLKAPTASQLIIPNFDGSITNYSGEEETEWLQEEVNIMKIKELTSTVNKYREQLAMVQSLNEHAESSLSKAERSKNYLTGRLQEVEELARGFQTTNADLQNELADAVVKQKEYEVLYVEKSNDYNTLLLQKEKLMKQIDEFHVIRVQDLEEREKKDQLLFQRYQKELNGFKVQLEEEREKNLRIRQDNRHMHAEIGDIRTKFDELVLEKTNLLKENSILQADLQSLSRVNNSSSTAQQNAQSQLLSLTAQLQEVREANQTLRKDQDTLLRENRNLERKLHEVSEQLNKKFDSSARPFDEIEMEKEVLTLKSNLAQKDDLLSSLVERIKQIEMFALKTQKDSNNHREENLQLHRQLGVLQKEKKDLELKLFDLDLKTYPISTSKDVRMLQKQISDLEASFAASDIERIKGIDECRNRDRTIRQLEAQISKFDDDKKRIQSSVSRLEERNAQLRNQLEDVQASETQWKFALRRTEHALQEERERVKSLETDFDKYRSLLEGQRVKRSESRLSMRSNRSPSVLR

Target ORF information:

RefSeq Version	NM_001019246.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Myo3-like myosin II heavy chain myp2 (myp2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001019246.2

ORF Insert Sequence:

ATGTCGTATC TTTCAAAAAA TGGCTCTAAC GACAACAATA ATATTATCAA AAAGCTAGTA 
GATGCTGAAA AACATTGCAA TGCCGTGAAA GACGCGTCTT TTGATGAACG GACATGGATC 
TGGATTCCTG ATTCGAAGGA ATCATTCGTG AAAGCTTGGA TAGTTGAAGA TTTAGGAGAA 
AAATATAGGG TGAAATTAGA AAGAGATGGC TCGGAAAGAA TTGTGGATGG ATTTGATGCT 
GAAAAAGTAA ATCCACCGAA GTTTGATATG GTTGATGACA TGGCTGCGTT GACATGTCTC 
AACGAACCAT CAGTAGTTAA CAACCTTACT CAGCGTTATG AGAAAGATTT AATATATACC 
TATTCAGGCT TGTTTCTCGT TGCTGTAAAC CCCTATTGTC ATTTACCAAT TTATGGCGAT 
GATGTTGTGC GAAAATACCA ATCTAAACAG TTTAAAGAAA CTAAACCCCA TATTTTTGGA 
ACAGCTGATG CCGCATATCG AAGTTTATTA GAAAGGAGGA TCAATCAATC TATTTTAGTT 
ACTGGTGAAT CAGGAGCTGG AAAAACTGAA ACTACTAAAA AGGTTATTCA GTACCTAACA 
TCGGTCACTG ATGCTTCCAC AAGCGATTCT CAACAGTTGG AGAAGAAAAT CCTTGAAACG 
AATCCTGTGT TGGAGGCTTT TGGGAATGCT CAGACTGTCC GGAATAACAA CTCATCCCGC 
TTTGGCAAGT TCATACGAAT TGAATTTTCT AATAATGGAA GCATCGTTGG AGCGAATTTA 
GACTGGTACC TTTTGGAAAA ATCTCGCGTT ATACATCCTT CCTCCAATGA ACGCAATTAT 
CACGTCTTTT ATCAACTACT TCGAGGTGCT GATGGATCAT TGCTTGAAAG TCTTTTCCTT 
GATCGATATG TTGACCATTA CTCATATTTA AAAAATGGTC TCAAGCATAT TAATGGAGTC 
GATGATGGCA AAGAATTTCA AAAATTATGC TTTGGTCTCA GAACTTTGGG GTTTGATAAT 
AACGAAATTC ACAGTTTGTT CTTAATTATA GCTTCAATTC TTCATATAGG AAATATTGAA 
GTAGCATCTG ATCGTTCGGG GCAAGCTAGA TTTCCTTCCT TAACGCAAAT TGATCAATTA 
TGCCATTTAC TCGAAATCCC TGTTGATGGA TTTGTTAATG CAGCTTTACA CCCAAAGTCC 
AAAGCTGGAC GAGAATGGAT TGTGACGGCA AGAACACGTG AACAAGTAGT CCATACACTA 
CAATCCTTAG CCAAAGGCCT TTATGAGCGT AATTTTGCGC ATTTAGTGAA ACGTCTGAAT 
CAGACTATGT ATTATTCTCA GTCTGAACAT GATGGGTTCA TTGGGGTTTT AGATATTGCT 
GGGTTTGAAA TTTTCACTTT TAATAGTTTT GAGCAACTCT GTATTAATTT TACAAATGAA 
AAGCTTCAGC AATTCTTTAA TCATTACATG TTCGTTCTGG AACAAGAAGA ATATACACAA 
GAACGCATCG AATGGGATTT TATTGACTAC GGAAATGATT TGCAGCCCAC CATAGATGCT 
ATCGAAAAAT CTGAGCCCAT TGGTATTTTT TCTTGCCTCG ACGAGGATTG TGTTATGCCA 
ATGGCTACTG ATGCTACATT CACAGAAAAA CTTCACCTTC TTTTTAAGGG TAAATCCGAT 
ATATACCGTC CTAAAAAGTT TTCTAGTGAA GGCTTTGTTT TAAAACATTA TGCTGGTGAT 
GTCGAATATG ATACCAAAGA TTGGCTGGAG AAAAACAAAG ATCCTCTAAA TGCATGTCTT 
GCCGCACTAA TGTTCAAATC TACTAACAGT CATGTGTCTT CTCTTTTTGA TGATTATTCG 
TCAAATGCTA GTGGTCGAGA TAACATTGAA AAGAAAGGAA TCTTTCGTAC TGTGTCGCAG 
CGTCATAGGA GACAGCTTTC TAGTCTTATG CACCAACTCG AAGCAACTCA GCCTCACTTT 
GTCAGATGCA TTATTCCAAA CAATTTGAAG CAGCCTCACA ATTTGGATAA ATCTCTCGTT 
CTTCATCAAC TTCGATGCAA CGGTGTACTG GAGGGTATTA GAATTGCTCA AACTGGATTT 
CCAAACAAAC TATTTTATAC AGAATTTAGA GCTCGTTATG GGATTCTTTC GCAGTCATTA 
AAGAGAGGAT ATGTCGAAGC AAAAAAGGCA ACAATAACGA TCATCAATGA ATTAAAATTA 
CCTAGTACCG TATACCGTTT GGGTGAAACA AAGGTATTTT TTAAAGCCAG TGTCTTGGGT 
TCTTTAGAGG ATCGCAGAAA CGCTTTACTG CGGGTGATAT TTAATTCATT TTCAGCTCGC 
ATTCGTGGGT TTTTGACAAG ACGGAGACTT TACCGGTTTA ATCATAGGCA AGATGCAGCT 
ATCCTTCTAC AGCATAACTT GAGGCAGTTG AAGTTATTGA AGCCACATCC TTGGTGGAAT 
CTTTTTCTGC ATCTTAAACC GTTATTGGGA ACAACCCAGA CTGATGAGTA TTTAAGAAGA 
AAAGACGCAC TCATAAATAA TTTACAGAAT CAATTGGAAA GTACCAAGGA AGTTGCAAAC 
GAATTAACTA TTACCAAAGA ACGAGTCTTA CAATTGACTA ACGATTTGCA AGAGGAACAA 
GCTCTGGCAC ATGAAAAGGA CATTTTGGTA GAAAGAGCCA ATAGCCGGGT TGAAGTTGTT 
CATGAGAGAC TGTCAAGTTT GGAAAATCAA GTAACTATTG CTGATGAAAA ATATGAGTTT 
TTGTATGCAG AGAAGCAAAG CATAGAGGAG GACCTTGCCA ACAAACAGAC AGAAATTTCT 
TATCTTTCCG ATTTATCATC TACATTGGAG AAAAAGCTGT CTTCAATTAA GAAGGACGAG 
CAAACGATTT CTTCTAAATA TAAAGAGCTT GAGAAAGACT ATCTGAATAT CATGGCGGAT 
TATCAACATA GTTCGCAGCA TTTATCAAAT CTTGAAAAGG CTATTAACGA AAAGAATTTA 
AATATTCGCG AATTGAATGA GAAACTCATG AGACTTGATG ATGAGTTACT ATTAAAGCAA 
CGAAGTTATG ATACAAAGGT TCAAGAATTA AGGGAAGAAA ACGCAAGCTT AAAAGACCAA 
TGTCGTACTT ACGAATCTCA ATTAGCAAGT CTTGTTTCCA AATACTCAGA AACAGAAAGT 
GAATTGAATA AGAAGGAAGC CGAGTTAGTT ATCTTTCAGA AGGAAATCAC GGAATACAGA 
GATCAACTTC ATAAAGCTTT TCAAAATCCT GAGAAAACTC ATAACATTAA TGATGTAAAA 
AGTGGCCCCT TAAACAGTGA TGAGAATATT TATTCTACTT CTTCGACCAC GCTTTCAATT 
TTGAAGGATG TTCAAGAGCT TAAATCATTA CATACGAAAG AAGCAAACCA GTTATCTGAA 
CGGATTAAAG AGATAAGTGA GATGCTTGAG CAAAGTATAG CGACTGAAGA AAAGCTTCGT 
AGAAAAAATT CTGAACTATG TGATATAATT GAGGCCTTAA AATACCAAAT TCAGGATCAG 
GAAACTGAAA TTATCTCTTT AAATGCTGAT AATCTGGATT TGAAAGATAC AAATGGTGTT 
TTGGAGAAGA ATGCTAGTGA TTTCATAGAC TTCCAAGGTA TAAAAAGCCG TTATGAACAT 
AAAATTTCAG ATTTACTGAA TCAGCTTCAG AAGGAAAGGT GTAAAGTTGG GCTATTAAAA 
CAAAAAACAG AAAATCGGTC AGTTACACAA CACACTTTAG ATGGAAATTC TCCACATCCA 
TCATTTGAAG AGAAACATTC AGGTGATCCT CTAAAACGCA TTGATGGTAA TAACGATGAT 
CGAAAAATTG ACAATAAATT ATTGAAAACA ATTAGTAAAA GCCTTGATGC TTTGCAACTA 
ACCGTTGAAG AAGAACTTAG CAATCTGTAT TCCCTCTCTA AGGATTTATC TTTTACTGAC 
ATTTCGGGAC ACATACCAAA CTCTATTAGG AAGCTTGAAA AAGGGTTGAG TACTTTATCA 
GAATTAAAGG AACGTCTCAA TGCATCAAAT TCTGACAGAC CTTCCCCGGA CATTTTTAAA 
GATACTCAAG CTATCATGAA TTCTCGGAAG CTATTAAGCA ATCCTAATTC TGATGCTCAA 
AGTGGTTTAA TATCATCATT GCAGAAAAAG CTTTATAATC CTGAATCTAA TATGGAATTT 
ACTGGGCTGA AACCTTTGTC TCCAAGCAAA ATCAGCAATT TACCCTCTTC TCAACCCGGC 
TCACCTTCAA AGAGATCTGG CAAAATGGAA GCACTGATTA GAAATTTTGA TCAAAATAGT 
AGTATTCCTG ATCCCTTTAT CGTTAATCAA CGGAATTCCG TGCTTCAAAC AGAGTTTGAA 
AAAATCAATT TGAAGTTAAA GGAAGCTACA AAGTCTGGAA TCTTAGATAA CAAAGATCTA 
TCAAAATTTT CGGAACTTAT ACAATCTTTG TTAAAAGAAA ATGAGGAACT TAAAAACTTA 
ACTACTAGTA ATTTAGGCAG TGATGATAAA ATGCTTGATT TTGCTCCATT ACTTGAAGAT 
GTTCCAAATA ATACTAGAAA CCAAATCAAA GGTTTTGTGG AAAAGGCAAT ATCATCGAAG 
CGAGCTATTG CAAAGTTATA TTCGGCAAGC GAAGAAAAGC TGTTTTCAAC GGAAAAAGCT 
TTAAGAGAGA TAACCAAGGA AAGAGATCGT TTGCTTCATG GGTTGCAAGG ACCTTCTGTT 
CCTACATCTC CTTTAAAGGC TCCCACTGCT TCTCAATTAA TAATTCCCAA TTTTGATGGT 
AGTATAACTA ATTATTCTGG AGAAGAAGAA ACCGAGTGGT TGCAAGAAGA AGTTAATATA 
ATGAAGATAA AGGAGCTTAC TTCTACAGTT AACAAGTACC GCGAACAGCT TGCGATGGTC 
CAGTCACTCA ATGAGCATGC AGAATCTTCA CTTTCTAAAG CTGAAAGGTC TAAAAATTAT 
CTTACCGGGC GTTTACAGGA AGTTGAAGAA CTCGCTCGCG GATTTCAAAC AACAAATGCT 
GATCTGCAAA ACGAACTGGC AGATGCTGTC GTGAAACAAA AGGAGTATGA AGTTCTTTAC 
GTTGAAAAGA GCAACGATTA TAATACTTTG CTGCTGCAAA AAGAAAAACT GATGAAACAA 
ATTGATGAGT TTCATGTCAT TCGTGTTCAA GATCTCGAGG AACGAGAAAA GAAGGATCAA 
CTACTCTTTC AACGTTATCA AAAGGAGCTA AATGGTTTCA AAGTACAGTT GGAAGAAGAA 
CGTGAGAAGA ACTTGCGCAT TAGACAAGAT AATCGGCACA TGCATGCGGA AATTGGTGAT 
ATCCGTACCA AATTTGACGA ATTAGTTTTG GAAAAGACAA ATTTGTTAAA AGAAAATTCA 
ATACTTCAAG CAGACTTACA ATCTCTTTCT CGTGTCAATA ATTCTTCCTC AACAGCGCAA 
CAAAATGCCC AAAGCCAACT TCTTTCTTTG ACTGCACAAC TCCAAGAAGT ACGAGAAGCA 
AACCAGACAT TAAGAAAAGA TCAAGATACC CTTTTAAGAG AAAATCGAAA CCTTGAGCGA 
AAACTTCACG AAGTTTCAGA GCAATTGAAC AAAAAATTCG ATAGTTCTGC AAGACCTTTT 
GATGAAATTG AAATGGAGAA AGAGGTATTA ACACTCAAGA GTAACCTTGC TCAAAAAGAT 
GACTTACTAT CATCATTAGT TGAACGCATA AAACAGATCG AAATGTTTGC GCTAAAAACT 
CAAAAAGATA GTAATAATCA TCGAGAAGAA AATCTTCAAC TGCATCGTCA ACTCGGAGTT 
TTACAAAAGG AGAAAAAGGA TTTGGAGCTC AAATTGTTTG ATTTAGATCT TAAAACTTAT 
CCCATATCAA CATCAAAGGA CGTCCGAATG CTACAAAAGC AAATATCAGA TTTGGAAGCA 
TCGTTTGCTG CTTCCGACAT CGAACGAATT AAAGGAATTG ATGAATGTCG CAATCGCGAT 
AGGACTATCC GTCAACTGGA AGCTCAAATA TCAAAATTTG ATGATGATAA AAAGAGAATA 
CAGAGCTCAG TTTCTCGTTT GGAGGAAAGA AATGCGCAAC TGAGAAATCA ATTAGAAGAT 
GTGCAAGCTT CAGAAACACA ATGGAAATTT GCACTACGTC GGACGGAACA TGCTCTTCAA 
GAAGAGCGTG AACGCGTCAA AAGTTTGGAA ACCGATTTTG ATAAATATAG ATCTTTGCTG 
GAGGGTCAAA GAGTAAAGAG AAGTGAAAGT AGATTATCCA TGAGGTCCAA TCGTTCGCCT 
AGCGTTCTAA GGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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myp2 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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