hrp3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	hrp3
Entrez Gene ID	2543064
Full Name	ATP-dependent DNA helicase Hrp3
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001020398.2	NP_594967.1	ATP-dependent DNA helicase Hrp3
NM_001356206.1	NP_001342861.1	ATP-dependent DNA helicase Hrp3

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHD1	XP_517850.2
	CHD1	XP_001097125.1
Mus musculus (house mouse)	Chd1	NP_031716.2
Eremothecium gossypii	AGOS_AGR123C	NP_986789.1
Rattus norvegicus (Norway rat)	Chd1	NP_001100935.1
Danio rerio (zebrafish)	zgc:173506	NP_001121770.2
Saccharomyces cerevisiae (baker's yeast)	CHD1	NP_011091.1
Schizosaccharomyces pombe (fission yeast)	hrp3	NP_594967.1
Homo sapiens (human)	CHD1	NP_001261.2
Canis lupus familiaris (dog)	CHD1	XP_848459.1
Gallus gallus (chicken)	CHD1	NP_990272.1
Kluyveromyces lactis	KLLA0C17578g	XP_452985.1
Schizosaccharomyces pombe (fission yeast)	hrp1	NP_593660.1
Bos taurus (cattle)	CHD1	NP_001178977.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

S. pombe lacks ISWI-type remodelers but it has two CHD1-type ATPases, Hrp1 and Hrp3
Title: CHD1 remodelers regulate nucleosome spacing in vitro and align nucleosomal arrays over gene coding regions in S. pombe.

Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin
Title: Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin.

deletion of hrp3 causes accumulation of antisense transcripts and affects nucleosome positioning.
Title: Chd1 chromatin remodelers maintain nucleosome organization and repress cryptic transcription.

The following hrp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the hrp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc102639	NM_001356206.1 Latest version!	Schizosaccharomyces pombe ATP-dependent DNA helicase Hrp3 (hrp3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OSj04238	NM_001020398.2 Latest version!	Schizosaccharomyces pombe 972h- ATP-dependent DNA helicase Hrp3 (hrp3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

hrp3 ( NM_001356206.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342861.1 Schizosaccharomyces pombe ATP-dependent DNA helicase Hrp3 (hrp3), mRNA.

Price & Availability↑

CloneID	OSc102639
Clone ID Related Accession (Same CDS sequence)	NM_001356206.1
Accession Version	NM_001356206.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	ATP-dependent DNA helicase Hrp3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424).

ATGAGTACAA GTGCTATAGC ACTTGCTTTG TCATCATCTA AGGCTATTGA GCAGCTGGAT 
CACGTTCAAA CTGAAACCCC TAATCTTAAG CAGGAAATGT CCGAATCCCC TTCGAATAGC 
GGCGTAGCCT CCAAGCGCAA ATTACAATCT ACTGAATGGC TCGACCCAGA GCTTTACGGT 
CTTCGACGTT CTGGTCGCAC TCGTAGTAAT CCAGGAAGAT ATGTAGACAC TGATGATCAA 
GAAGATGTGT TCCCATCCAA GCATCGTAAA GGTACTAGAA ATGGTTCATC TTTTTCTCGT 
CATCGAACAA TTCGCGATTT AGACGATGAA GCTGAATCTG TCACCTCTGA GGAAAGTGAG 
TCAGATGATA GTTCTTATGG CGGAACTCCC AAAAAGCGAT CTCGGCAAAA AAAATCAAAC 
ACCTACGTTC AAGATGAAAT TCGATTTTCT TCTAGAAATA GTAAAGGTGT TAACTATAAC 
GAAGACGCGT ATTTTGAATC GTTTGAAGAA GAAGAGGAAG AAGAGATGTA TGAGTACGCA 
ACTGAAGTTT CTGAAGAACC TGAAGATACA CGAGCTATAG ATGTCGTTTT AGATCATAGA 
CTTATTGAAG GACACGATGG CTCTACACCT TCTGAAGATT ATGAGTTTTT AATTAAATGG 
GTTAATTTTT CTCATCTTCA TTGTACATGG GAACCTTACA ATAACATTTC TATGATCAGA 
GGATCTAAAA AGGTCGACAA TCACATTAAG CAAGTCATAC TTCTTGATCG AGAAATTCGA 
GAAGATCCGA CCACTACGCG TGAAGACATT GAAGCAATGG ATATTGAAAA AGAGAGAAAG 
CGTGAAAATT ACGAGGAATA TAAGCAGGTA GACAGAATTG TTGCCAAACA CTTAAACTCC 
GATGGCTCAG TTGAATACTT AGTGAAATGG AAGCAGCTGT TATATGATTT TTGCACATGG 
GAAGCTTCCT CAATTATTGA GCCTATTGCT GCCACGGAAA TTCAGGCATT TCAAGAAAGA 
GAAGAAAGTG CTCTTAGTCC ATCAAGAGGT ACAAACTATG GTAATTCGCG TCCTAAGTAT 
CGAAAACTGG AGCAACAGCC TTCGTATATA ACCGGTGGTG AGCTTCGTGA CTTTCAGCTT 
ACAGGCGTCA ACTGGATGGC ATATCTTTGG CATAAAAATG AAAATGGGAT TTTGGCCGAT 
GAAATGGGTC TCGGTAAGAC AGTCCAAACT GTGGCTTTTC TTTCATATCT TGCACATTCT 
TTAAGACAGC ATGGACCTTT TCTTGTAGTT GTTCCACTTT CTACAGTTCC TGCCTGGCAA 
GAGACATTAG CTCTTTGGGC TTCTGATATG AATTGTATAA GTTACCTTGG AAATACCACC 
TCAAGGCAAG TAATAAGGGA TTACGAATTT TATGTTGATG GAACTCAGAA AATAAAGTTC 
AACTTGCTTC TAACTACTTA TGAGTATGTA CTCAAAGATC GCTCGGTGCT TAGTAATATA 
AAATGGCAAT ATATGGCAAT TGACGAAGCT CATCGATTAA AAAACAGTGA ATCGTCACTT 
TATGAAGCAC TTTCTCAGTT CAAGAACTCA AATCGATTAC TTATAACTGG AACACCATTA 
CAAAATAATA TTCGTGAGCT TGCGGCTTTG GTAGACTTCC TGATGCCAGG AAAGTTTGAG 
ATTCGTGAGG AAATTAATTT AGAAGCACCA GATGAGGAGC AGGAAGCTTA TATTAGAAGT 
TTACAGGAGC ATTTACAACC CTACATTTTA CGAAGATTAA AGAAAGACGT TGAGAAATCT 
CTTCCTTCTA AGTCTGAACG TATCCTTCGT GTTGAGCTTT CTGACTTACA AATGTACTGG 
TACAAAAATA TTTTAACTCG CAATTACCGT GTCCTTACTC AAAGCATAAG TTCGGGAAGT 
CAAATATCTT TACTAAACAT AGTTGTGGAG TTGAAAAAGG CAAGTAATCA TCCTTACTTA 
TTTGACGGAG TAGAAGAGTC ATGGATGCAA AAAATAAACT CTCAAGGAAG ACGAGATGAA 
GTCTTAAAAG GTTTAATTAT GAACAGCGGG AAAATGGTGT TACTTGACAA ACTTCTATCA 
CGTCTTCGTC GAGACGGCCA CCGCGTTTTA ATTTTTAGTC AAATGGTTCG TATGCTTGAC 
ATTCTTGGTG ATTATCTTTC TTTGAGGGGA TATCCACATC AGAGACTGGA TGGCACTGTC 
CCTGCTGCTG TACGAAGAAC TTCAATAGAT CATTTTAATG CACCAAACAG CCCAGATTTT 
GTGTTTTTAT TATCCACACG AGCTGGAGGA TTAGGTATTA ATTTAATGAC TGCTGATACA 
GTTATTATTT TTGATTCCGA TTGGAACCCT CAAGCTGATT TACAAGCTAT GGCACGTGCG 
CATCGCATTG GTCAGAAAAA TCATGTTATG GTATACAGGC TTCTTTCCAA AGATACGATT 
GAAGAAGATG TCTTGGAACG TGCACGTCGT AAAATGATTC TTGAATATGC AATTATTTCA 
TTAGGTGTTA CAGACAAGCA AAAGAATTCC AAAAATGATA AATTTTCTGC TGAAGAGCTT 
AGCGCTATTT TGAAATTTGG AGCTTCTAAT ATGTTTAAAG CAGAGAATAA TCAAAAGAAG 
CTTGAGGATA TGAATTTAGA TGAAATTTTG GAACATGCCG AAGATCATGA TACTTCCAAT 
GATGTGGGTG GAGCCAGCAT GGGTGGTGAA GAGTTTTTGA AACAGTTCGA AGTTACAGAC 
TATAAGGCAG ATGTATCTTG GGATGATATT ATACCCCTAA CTGAACGTGA GAAGTTTGAA 
GAGGAAGACC GCTTAAGAGA AGAGGAAGAA GCGCTTAAGC AAGAAATTGA GCTTAGTAGT 
AGACGAGGTA ATCGTCCTTA TCCTAGCTCT GCGGTGGAAT CCCCATCCTA CTCTGGAACT 
TCTGAAAGGA AGAGCAAAAA GCAAATGCTT AAAGATGAGG TTTTACTGGA AAAGGAAATT 
CGGTTGCTTT ATCGTGCTAT GATTAGATAT GGAAGCCTTG AGCATCGCTA CAATGACATT 
GTTAAATATG CTGATCTTAC TACGCAAGAT GCTCACGTAA TAAAAAAGAT TGCAGCTGAC 
TTAGTAACCG CTTCACGCAA GGCTGTATCA GCAGCCGAGA AGGACTTATC TAATGATCAG 
TCTAATAATA AGTCATCTCG TAAAGCGCTG CTCATTACAT TTAAAGGTGT AAAAAATATA 
AATGCTGAAA CATTGGTACA AAGGCTAAAT GATCTTGATA TTCTTTATGA TGCTATGCCA 
ACGTCAGGAT ATTCTAACTT CCAAATTCCA ATGCACGTTA GATCTGTTCA CGGCTGGTCC 
TGTCAGTGGG GACCCCGAGA AGATTCCATG TTGCTTTCTG GAATTTGTAA GCATGGATTT 
GGAGCTTGGT TAGAAATTCG AGATGATCCA GAACTCAAAA TGAAAGACAA GATCTTTTTA 
GAAGACACAA AACAAACAGA CAACTCTGTC CCCAAAGATA AAGAAAATAA AGAGAAAAAG 
GTACCTTCAG CTGTGCATTT GGTTCGTCGA GGTGAATACC TACTTAGTGC TTTACGTGAG 
CATCACCAAA ATTTTGGCAT AAAATCAAGC CCCGCTATTA GTACTAATGG AAAAACTCAA 
CCTAAGAAGC AGACGGCTAA TCGCAGACAA AGTGGCAAGC CTAACGTTAA ATCTGCGCAA 
AAAATAGAGA GTGCAACTAG GACACCATCG CCTGCAATTT CAGAATCTCG TAAGAAACCT 
TCTTCTAAAG ACACGAAGAT TGAAACACCT AGTAGGGAAC AGTCTCGCAG CCAGACAGCT 
TCACCCGTCA AATCTGAAAA AGACGATGGT AATGTAAGTC TAAATGCTGA ACAAAAGGCA 
CGCTGCAAAG AACTGATGTA TCCAGTCCGT AAGCATATGA AAAGACTTCG GAAAGATTCT 
AGTGGGCTTG GACGCGCGGA GTTGGTGAAG TTACTTACTG AGTGTTTGAC AACGATTGGA 
AAGCATATTG AAAAAACTGT CAATGATACA CCCTCCGAAG AAAAAGCTAC TGTTAGGAAG 
AATTTATGGA TGTTTGCATG TTACTTTTGG CCGAAGGAGG AGGTTAAATA TACCAGCTTG 
ATCAGCATGT ATGAAAAGAT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342861.1
CDS	104..4270
Translation	MSTSAIALALSSSKAIEQLDHVQTETPNLKQEMSESPSNSGVASKRKLQSTEWLDPELYGLRRSGRTRSNPGRYVDTDDQEDVFPSKHRKGTRNGSSFSRHRTIRDLDDEAESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEEEEEEEMYEYATEVSEEPEDTRAIDVVLDHRLIEGHDGSTPSEDYEFLIKWVNFSHLHCTWEPYNNISMIRGSKKVDNHIKQVILLDREIREDPTTTREDIEAMDIEKERKRENYEEYKQVDRIVAKHLNSDGSVEYLVKWKQLLYDFCTWEASSIIEPIAATEIQAFQEREESALSPSRGTNYGNSRPKYRKLEQQPSYITGGELRDFQLTGVNWMAYLWHKNENGILADEMGLGKTVQTVAFLSYLAHSLRQHGPFLVVVPLSTVPAWQETLALWASDMNCISYLGNTTSRQVIRDYEFYVDGTQKIKFNLLLTTYEYVLKDRSVLSNIKWQYMAIDEAHRLKNSESSLYEALSQFKNSNRLLITGTPLQNNIRELAALVDFLMPGKFEIREEINLEAPDEEQEAYIRSLQEHLQPYILRRLKKDVEKSLPSKSERILRVELSDLQMYWYKNILTRNYRVLTQSISSGSQISLLNIVVELKKASNHPYLFDGVEESWMQKINSQGRRDEVLKGLIMNSGKMVLLDKLLSRLRRDGHRVLIFSQMVRMLDILGDYLSLRGYPHQRLDGTVPAAVRRTSIDHFNAPNSPDFVFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVMVYRLLSKDTIEEDVLERARRKMILEYAIISLGVTDKQKNSKNDKFSAEELSAILKFGASNMFKAENNQKKLEDMNLDEILEHAEDHDTSNDVGGASMGGEEFLKQFEVTDYKADVSWDDIIPLTEREKFEEEDRLREEEEALKQEIELSSRRGNRPYPSSAVESPSYSGTSERKSKKQMLKDEVLLEKEIRLLYRAMIRYGSLEHRYNDIVKYADLTTQDAHVIKKIAADLVTASRKAVSAAEKDLSNDQSNNKSSRKALLITFKGVKNINAETLVQRLNDLDILYDAMPTSGYSNFQIPMHVRSVHGWSCQWGPREDSMLLSGICKHGFGAWLEIRDDPELKMKDKIFLEDTKQTDNSVPKDKENKEKKVPSAVHLVRRGEYLLSALREHHQNFGIKSSPAISTNGKTQPKKQTANRRQSGKPNVKSAQKIESATRTPSPAISESRKKPSSKDTKIETPSREQSRSQTASPVKSEKDDGNVSLNAEQKARCKELMYPVRKHMKRLRKDSSGLGRAELVKLLTECLTTIGKHIEKTVNDTPSEEKATVRKNLWMFACYFWPKEEVKYTSLISMYEKMK

Target ORF information:

RefSeq Version	NM_001356206.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe ATP-dependent DNA helicase Hrp3 (hrp3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001356206.1

ORF Insert Sequence:

ATGAGTACAA GTGCTATAGC ACTTGCTTTG TCATCATCTA AGGCTATTGA GCAGCTGGAT 
CACGTTCAAA CTGAAACCCC TAATCTTAAG CAGGAAATGT CCGAATCCCC TTCGAATAGC 
GGCGTAGCCT CCAAGCGCAA ATTACAATCT ACTGAATGGC TCGACCCAGA GCTTTACGGT 
CTTCGACGTT CTGGTCGCAC TCGTAGTAAT CCAGGAAGAT ATGTAGACAC TGATGATCAA 
GAAGATGTGT TCCCATCCAA GCATCGTAAA GGTACTAGAA ATGGTTCATC TTTTTCTCGT 
CATCGAACAA TTCGCGATTT AGACGATGAA GCTGAATCTG TCACCTCTGA GGAAAGTGAG 
TCAGATGATA GTTCTTATGG CGGAACTCCC AAAAAGCGAT CTCGGCAAAA AAAATCAAAC 
ACCTACGTTC AAGATGAAAT TCGATTTTCT TCTAGAAATA GTAAAGGTGT TAACTATAAC 
GAAGACGCGT ATTTTGAATC GTTTGAAGAA GAAGAGGAAG AAGAGATGTA TGAGTACGCA 
ACTGAAGTTT CTGAAGAACC TGAAGATACA CGAGCTATAG ATGTCGTTTT AGATCATAGA 
CTTATTGAAG GACACGATGG CTCTACACCT TCTGAAGATT ATGAGTTTTT AATTAAATGG 
GTTAATTTTT CTCATCTTCA TTGTACATGG GAACCTTACA ATAACATTTC TATGATCAGA 
GGATCTAAAA AGGTCGACAA TCACATTAAG CAAGTCATAC TTCTTGATCG AGAAATTCGA 
GAAGATCCGA CCACTACGCG TGAAGACATT GAAGCAATGG ATATTGAAAA AGAGAGAAAG 
CGTGAAAATT ACGAGGAATA TAAGCAGGTA GACAGAATTG TTGCCAAACA CTTAAACTCC 
GATGGCTCAG TTGAATACTT AGTGAAATGG AAGCAGCTGT TATATGATTT TTGCACATGG 
GAAGCTTCCT CAATTATTGA GCCTATTGCT GCCACGGAAA TTCAGGCATT TCAAGAAAGA 
GAAGAAAGTG CTCTTAGTCC ATCAAGAGGT ACAAACTATG GTAATTCGCG TCCTAAGTAT 
CGAAAACTGG AGCAACAGCC TTCGTATATA ACCGGTGGTG AGCTTCGTGA CTTTCAGCTT 
ACAGGCGTCA ACTGGATGGC ATATCTTTGG CATAAAAATG AAAATGGGAT TTTGGCCGAT 
GAAATGGGTC TCGGTAAGAC AGTCCAAACT GTGGCTTTTC TTTCATATCT TGCACATTCT 
TTAAGACAGC ATGGACCTTT TCTTGTAGTT GTTCCACTTT CTACAGTTCC TGCCTGGCAA 
GAGACATTAG CTCTTTGGGC TTCTGATATG AATTGTATAA GTTACCTTGG AAATACCACC 
TCAAGGCAAG TAATAAGGGA TTACGAATTT TATGTTGATG GAACTCAGAA AATAAAGTTC 
AACTTGCTTC TAACTACTTA TGAGTATGTA CTCAAAGATC GCTCGGTGCT TAGTAATATA 
AAATGGCAAT ATATGGCAAT TGACGAAGCT CATCGATTAA AAAACAGTGA ATCGTCACTT 
TATGAAGCAC TTTCTCAGTT CAAGAACTCA AATCGATTAC TTATAACTGG AACACCATTA 
CAAAATAATA TTCGTGAGCT TGCGGCTTTG GTAGACTTCC TGATGCCAGG AAAGTTTGAG 
ATTCGTGAGG AAATTAATTT AGAAGCACCA GATGAGGAGC AGGAAGCTTA TATTAGAAGT 
TTACAGGAGC ATTTACAACC CTACATTTTA CGAAGATTAA AGAAAGACGT TGAGAAATCT 
CTTCCTTCTA AGTCTGAACG TATCCTTCGT GTTGAGCTTT CTGACTTACA AATGTACTGG 
TACAAAAATA TTTTAACTCG CAATTACCGT GTCCTTACTC AAAGCATAAG TTCGGGAAGT 
CAAATATCTT TACTAAACAT AGTTGTGGAG TTGAAAAAGG CAAGTAATCA TCCTTACTTA 
TTTGACGGAG TAGAAGAGTC ATGGATGCAA AAAATAAACT CTCAAGGAAG ACGAGATGAA 
GTCTTAAAAG GTTTAATTAT GAACAGCGGG AAAATGGTGT TACTTGACAA ACTTCTATCA 
CGTCTTCGTC GAGACGGCCA CCGCGTTTTA ATTTTTAGTC AAATGGTTCG TATGCTTGAC 
ATTCTTGGTG ATTATCTTTC TTTGAGGGGA TATCCACATC AGAGACTGGA TGGCACTGTC 
CCTGCTGCTG TACGAAGAAC TTCAATAGAT CATTTTAATG CACCAAACAG CCCAGATTTT 
GTGTTTTTAT TATCCACACG AGCTGGAGGA TTAGGTATTA ATTTAATGAC TGCTGATACA 
GTTATTATTT TTGATTCCGA TTGGAACCCT CAAGCTGATT TACAAGCTAT GGCACGTGCG 
CATCGCATTG GTCAGAAAAA TCATGTTATG GTATACAGGC TTCTTTCCAA AGATACGATT 
GAAGAAGATG TCTTGGAACG TGCACGTCGT AAAATGATTC TTGAATATGC AATTATTTCA 
TTAGGTGTTA CAGACAAGCA AAAGAATTCC AAAAATGATA AATTTTCTGC TGAAGAGCTT 
AGCGCTATTT TGAAATTTGG AGCTTCTAAT ATGTTTAAAG CAGAGAATAA TCAAAAGAAG 
CTTGAGGATA TGAATTTAGA TGAAATTTTG GAACATGCCG AAGATCATGA TACTTCCAAT 
GATGTGGGTG GAGCCAGCAT GGGTGGTGAA GAGTTTTTGA AACAGTTCGA AGTTACAGAC 
TATAAGGCAG ATGTATCTTG GGATGATATT ATACCCCTAA CTGAACGTGA GAAGTTTGAA 
GAGGAAGACC GCTTAAGAGA AGAGGAAGAA GCGCTTAAGC AAGAAATTGA GCTTAGTAGT 
AGACGAGGTA ATCGTCCTTA TCCTAGCTCT GCGGTGGAAT CCCCATCCTA CTCTGGAACT 
TCTGAAAGGA AGAGCAAAAA GCAAATGCTT AAAGATGAGG TTTTACTGGA AAAGGAAATT 
CGGTTGCTTT ATCGTGCTAT GATTAGATAT GGAAGCCTTG AGCATCGCTA CAATGACATT 
GTTAAATATG CTGATCTTAC TACGCAAGAT GCTCACGTAA TAAAAAAGAT TGCAGCTGAC 
TTAGTAACCG CTTCACGCAA GGCTGTATCA GCAGCCGAGA AGGACTTATC TAATGATCAG 
TCTAATAATA AGTCATCTCG TAAAGCGCTG CTCATTACAT TTAAAGGTGT AAAAAATATA 
AATGCTGAAA CATTGGTACA AAGGCTAAAT GATCTTGATA TTCTTTATGA TGCTATGCCA 
ACGTCAGGAT ATTCTAACTT CCAAATTCCA ATGCACGTTA GATCTGTTCA CGGCTGGTCC 
TGTCAGTGGG GACCCCGAGA AGATTCCATG TTGCTTTCTG GAATTTGTAA GCATGGATTT 
GGAGCTTGGT TAGAAATTCG AGATGATCCA GAACTCAAAA TGAAAGACAA GATCTTTTTA 
GAAGACACAA AACAAACAGA CAACTCTGTC CCCAAAGATA AAGAAAATAA AGAGAAAAAG 
GTACCTTCAG CTGTGCATTT GGTTCGTCGA GGTGAATACC TACTTAGTGC TTTACGTGAG 
CATCACCAAA ATTTTGGCAT AAAATCAAGC CCCGCTATTA GTACTAATGG AAAAACTCAA 
CCTAAGAAGC AGACGGCTAA TCGCAGACAA AGTGGCAAGC CTAACGTTAA ATCTGCGCAA 
AAAATAGAGA GTGCAACTAG GACACCATCG CCTGCAATTT CAGAATCTCG TAAGAAACCT 
TCTTCTAAAG ACACGAAGAT TGAAACACCT AGTAGGGAAC AGTCTCGCAG CCAGACAGCT 
TCACCCGTCA AATCTGAAAA AGACGATGGT AATGTAAGTC TAAATGCTGA ACAAAAGGCA 
CGCTGCAAAG AACTGATGTA TCCAGTCCGT AAGCATATGA AAAGACTTCG GAAAGATTCT 
AGTGGGCTTG GACGCGCGGA GTTGGTGAAG TTACTTACTG AGTGTTTGAC AACGATTGGA 
AAGCATATTG AAAAAACTGT CAATGATACA CCCTCCGAAG AAAAAGCTAC TGTTAGGAAG 
AATTTATGGA TGTTTGCATG TTACTTTTGG CCGAAGGAGG AGGTTAAATA TACCAGCTTG 
ATCAGCATGT ATGAAAAGAT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

hrp3 ( NM_001020398.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594967.1 Schizosaccharomyces pombe 972h- ATP-dependent DNA helicase Hrp3 (hrp3), mRNA.

Price & Availability↑

CloneID	OSj04238
Clone ID Related Accession (Same CDS sequence)	NM_001020398.2
Accession Version	NM_001020398.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	ATP-dependent DNA helicase Hrp3
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020398.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGAGTACAA GTGCTATAGC ACTTGCTTTG TCATCATCTA AGGCTATTGA GCAGCTGGAT 
CACGTTCAAA CTGAAACCCC TAATCTTAAG CAGGAAATGT CCGAATCCCC TTCGAATAGC 
GGCGTAGCCT CCAAGCGCAA ATTACAATCT ACTGAATGGC TCGACCCAGA GCTTTACGGT 
CTTCGACGTT CTGGTCGCAC TCGTAGTAAT CCAGGAAGAT ATGTAGACAC TGATGATCAA 
GAAGATGTGT TCCCATCCAA GCATCGTAAA GGTACTAGAA ATGGTTCATC TTTTTCTCGT 
CATCGAACAA TTCGCGATTT AGACGATGAA GCTGAATCTG TCACCTCTGA GGAAAGTGAG 
TCAGATGATA GTTCTTATGG CGGAACTCCC AAAAAGCGAT CTCGGCAAAA AAAATCAAAC 
ACCTACGTTC AAGATGAAAT TCGATTTTCT TCTAGAAATA GTAAAGGTGT TAACTATAAC 
GAAGACGCGT ATTTTGAATC GTTTGAAGAA GAAGAGGAAG AAGAGATGTA TGAGTACGCA 
ACTGAAGTTT CTGAAGAACC TGAAGATACA CGAGCTATAG ATGTCGTTTT AGATCATAGA 
CTTATTGAAG GACACGATGG CTCTACACCT TCTGAAGATT ATGAGTTTTT AATTAAATGG 
GTTAATTTTT CTCATCTTCA TTGTACATGG GAACCTTACA ATAACATTTC TATGATCAGA 
GGATCTAAAA AGGTCGACAA TCACATTAAG CAAGTCATAC TTCTTGATCG AGAAATTCGA 
GAAGATCCGA CCACTACGCG TGAAGACATT GAAGCAATGG ATATTGAAAA AGAGAGAAAG 
CGTGAAAATT ACGAGGAATA TAAGCAGGTA GACAGAATTG TTGCCAAACA CTTAAACTCC 
GATGGCTCAG TTGAATACTT AGTGAAATGG AAGCAGCTGT TATATGATTT TTGCACATGG 
GAAGCTTCCT CAATTATTGA GCCTATTGCT GCCACGGAAA TTCAGGCATT TCAAGAAAGA 
GAAGAAAGTG CTCTTAGTCC ATCAAGAGGT ACAAACTATG GTAATTCGCG TCCTAAGTAT 
CGAAAACTGG AGCAACAGCC TTCGTATATA ACCGGTGGTG AGCTTCGTGA CTTTCAGCTT 
ACAGGCGTCA ACTGGATGGC ATATCTTTGG CATAAAAATG AAAATGGGAT TTTGGCCGAT 
GAAATGGGTC TCGGTAAGAC AGTCCAAACT GTGGCTTTTC TTTCATATCT TGCACATTCT 
TTAAGACAGC ATGGACCTTT TCTTGTAGTT GTTCCACTTT CTACAGTTCC TGCCTGGCAA 
GAGACATTAG CTCTTTGGGC TTCTGATATG AATTGTATAA GTTACCTTGG AAATACCACC 
TCAAGGCAAG TAATAAGGGA TTACGAATTT TATGTTGATG GAACTCAGAA AATAAAGTTC 
AACTTGCTTC TAACTACTTA TGAGTATGTA CTCAAAGATC GCTCGGTGCT TAGTAATATA 
AAATGGCAAT ATATGGCAAT TGACGAAGCT CATCGATTAA AAAACAGTGA ATCGTCACTT 
TATGAAGCAC TTTCTCAGTT CAAGAACTCA AATCGATTAC TTATAACTGG AACACCATTA 
CAAAATAATA TTCGTGAGCT TGCGGCTTTG GTAGACTTCC TGATGCCAGG AAAGTTTGAG 
ATTCGTGAGG AAATTAATTT AGAAGCACCA GATGAGGAGC AGGAAGCTTA TATTAGAAGT 
TTACAGGAGC ATTTACAACC CTACATTTTA CGAAGATTAA AGAAAGACGT TGAGAAATCT 
CTTCCTTCTA AGTCTGAACG TATCCTTCGT GTTGAGCTTT CTGACTTACA AATGTACTGG 
TACAAAAATA TTTTAACTCG CAATTACCGT GTCCTTACTC AAAGCATAAG TTCGGGAAGT 
CAAATATCTT TACTAAACAT AGTTGTGGAG TTGAAAAAGG CAAGTAATCA TCCTTACTTA 
TTTGACGGAG TAGAAGAGTC ATGGATGCAA AAAATAAACT CTCAAGGAAG ACGAGATGAA 
GTCTTAAAAG GTTTAATTAT GAACAGCGGG AAAATGGTGT TACTTGACAA ACTTCTATCA 
CGTCTTCGTC GAGACGGCCA CCGCGTTTTA ATTTTTAGTC AAATGGTTCG TATGCTTGAC 
ATTCTTGGTG ATTATCTTTC TTTGAGGGGA TATCCACATC AGAGACTGGA TGGCACTGTC 
CCTGCTGCTG TACGAAGAAC TTCAATAGAT CATTTTAATG CACCAAACAG CCCAGATTTT 
GTGTTTTTAT TATCCACACG AGCTGGAGGA TTAGGTATTA ATTTAATGAC TGCTGATACA 
GTTATTATTT TTGATTCCGA TTGGAACCCT CAAGCTGATT TACAAGCTAT GGCACGTGCG 
CATCGCATTG GTCAGAAAAA TCATGTTATG GTATACAGGC TTCTTTCCAA AGATACGATT 
GAAGAAGATG TCTTGGAACG TGCACGTCGT AAAATGATTC TTGAATATGC AATTATTTCA 
TTAGGTGTTA CAGACAAGCA AAAGAATTCC AAAAATGATA AATTTTCTGC TGAAGAGCTT 
AGCGCTATTT TGAAATTTGG AGCTTCTAAT ATGTTTAAAG CAGAGAATAA TCAAAAGAAG 
CTTGAGGATA TGAATTTAGA TGAAATTTTG GAACATGCCG AAGATCATGA TACTTCCAAT 
GATGTGGGTG GAGCCAGCAT GGGTGGTGAA GAGTTTTTGA AACAGTTCGA AGTTACAGAC 
TATAAGGCAG ATGTATCTTG GGATGATATT ATACCCCTAA CTGAACGTGA GAAGTTTGAA 
GAGGAAGACC GCTTAAGAGA AGAGGAAGAA GCGCTTAAGC AAGAAATTGA GCTTAGTAGT 
AGACGAGGTA ATCGTCCTTA TCCTAGCTCT GCGGTGGAAT CCCCATCCTA CTCTGGAACT 
TCTGAAAGGA AGAGCAAAAA GCAAATGCTT AAAGATGAGG TTTTACTGGA AAAGGAAATT 
CGGTTGCTTT ATCGTGCTAT GATTAGATAT GGAAGCCTTG AGCATCGCTA CAATGACATT 
GTTAAATATG CTGATCTTAC TACGCAAGAT GCTCACGTAA TAAAAAAGAT TGCAGCTGAC 
TTAGTAACCG CTTCACGCAA GGCTGTATCA GCAGCCGAGA AGGACTTATC TAATGATCAG 
TCTAATAATA AGTCATCTCG TAAAGCGCTG CTCATTACAT TTAAAGGTGT AAAAAATATA 
AATGCTGAAA CATTGGTACA AAGGCTAAAT GATCTTGATA TTCTTTATGA TGCTATGCCA 
ACGTCAGGAT ATTCTAACTT CCAAATTCCA ATGCACGTTA GATCTGTTCA CGGCTGGTCC 
TGTCAGTGGG GACCCCGAGA AGATTCCATG TTGCTTTCTG GAATTTGTAA GCATGGATTT 
GGAGCTTGGT TAGAAATTCG AGATGATCCA GAACTCAAAA TGAAAGACAA GATCTTTTTA 
GAAGACACAA AACAAACAGA CAACTCTGTC CCCAAAGATA AAGAAAATAA AGAGAAAAAG 
GTACCTTCAG CTGTGCATTT GGTTCGTCGA GGTGAATACC TACTTAGTGC TTTACGTGAG 
CATCACCAAA ATTTTGGCAT AAAATCAAGC CCCGCTATTA GTACTAATGG AAAAACTCAA 
CCTAAGAAGC AGACGGCTAA TCGCAGACAA AGTGGCAAGC CTAACGTTAA ATCTGCGCAA 
AAAATAGAGA GTGCAACTAG GACACCATCG CCTGCAATTT CAGAATCTCG TAAGAAACCT 
TCTTCTAAAG ACACGAAGAT TGAAACACCT AGTAGGGAAC AGTCTCGCAG CCAGACAGCT 
TCACCCGTCA AATCTGAAAA AGACGATGGT AATGTAAGTC TAAATGCTGA ACAAAAGGCA 
CGCTGCAAAG AACTGATGTA TCCAGTCCGT AAGCATATGA AAAGACTTCG GAAAGATTCT 
AGTGGGCTTG GACGCGCGGA GTTGGTGAAG TTACTTACTG AGTGTTTGAC AACGATTGGA 
AAGCATATTG AAAAAACTGT CAATGATACA CCCTCCGAAG AAAAAGCTAC TGTTAGGAAG 
AATTTATGGA TGTTTGCATG TTACTTTTGG CCGAAGGAGG AGGTTAAATA TACCAGCTTG 
ATCAGCATGT ATGAAAAGAT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_594967.1
CDS	599..4765
Translation	MSTSAIALALSSSKAIEQLDHVQTETPNLKQEMSESPSNSGVASKRKLQSTEWLDPELYGLRRSGRTRSNPGRYVDTDDQEDVFPSKHRKGTRNGSSFSRHRTIRDLDDEAESVTSEESESDDSSYGGTPKKRSRQKKSNTYVQDEIRFSSRNSKGVNYNEDAYFESFEEEEEEEMYEYATEVSEEPEDTRAIDVVLDHRLIEGHDGSTPSEDYEFLIKWVNFSHLHCTWEPYNNISMIRGSKKVDNHIKQVILLDREIREDPTTTREDIEAMDIEKERKRENYEEYKQVDRIVAKHLNSDGSVEYLVKWKQLLYDFCTWEASSIIEPIAATEIQAFQEREESALSPSRGTNYGNSRPKYRKLEQQPSYITGGELRDFQLTGVNWMAYLWHKNENGILADEMGLGKTVQTVAFLSYLAHSLRQHGPFLVVVPLSTVPAWQETLALWASDMNCISYLGNTTSRQVIRDYEFYVDGTQKIKFNLLLTTYEYVLKDRSVLSNIKWQYMAIDEAHRLKNSESSLYEALSQFKNSNRLLITGTPLQNNIRELAALVDFLMPGKFEIREEINLEAPDEEQEAYIRSLQEHLQPYILRRLKKDVEKSLPSKSERILRVELSDLQMYWYKNILTRNYRVLTQSISSGSQISLLNIVVELKKASNHPYLFDGVEESWMQKINSQGRRDEVLKGLIMNSGKMVLLDKLLSRLRRDGHRVLIFSQMVRMLDILGDYLSLRGYPHQRLDGTVPAAVRRTSIDHFNAPNSPDFVFLLSTRAGGLGINLMTADTVIIFDSDWNPQADLQAMARAHRIGQKNHVMVYRLLSKDTIEEDVLERARRKMILEYAIISLGVTDKQKNSKNDKFSAEELSAILKFGASNMFKAENNQKKLEDMNLDEILEHAEDHDTSNDVGGASMGGEEFLKQFEVTDYKADVSWDDIIPLTEREKFEEEDRLREEEEALKQEIELSSRRGNRPYPSSAVESPSYSGTSERKSKKQMLKDEVLLEKEIRLLYRAMIRYGSLEHRYNDIVKYADLTTQDAHVIKKIAADLVTASRKAVSAAEKDLSNDQSNNKSSRKALLITFKGVKNINAETLVQRLNDLDILYDAMPTSGYSNFQIPMHVRSVHGWSCQWGPREDSMLLSGICKHGFGAWLEIRDDPELKMKDKIFLEDTKQTDNSVPKDKENKEKKVPSAVHLVRRGEYLLSALREHHQNFGIKSSPAISTNGKTQPKKQTANRRQSGKPNVKSAQKIESATRTPSPAISESRKKPSSKDTKIETPSREQSRSQTASPVKSEKDDGNVSLNAEQKARCKELMYPVRKHMKRLRKDSSGLGRAELVKLLTECLTTIGKHIEKTVNDTPSEEKATVRKNLWMFACYFWPKEEVKYTSLISMYEKMK

Target ORF information:

RefSeq Version	NM_001020398.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- ATP-dependent DNA helicase Hrp3 (hrp3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001020398.2

ORF Insert Sequence:

ATGAGTACAA GTGCTATAGC ACTTGCTTTG TCATCATCTA AGGCTATTGA GCAGCTGGAT 
CACGTTCAAA CTGAAACCCC TAATCTTAAG CAGGAAATGT CCGAATCCCC TTCGAATAGC 
GGCGTAGCCT CCAAGCGCAA ATTACAATCT ACTGAATGGC TCGACCCAGA GCTTTACGGT 
CTTCGACGTT CTGGTCGCAC TCGTAGTAAT CCAGGAAGAT ATGTAGACAC TGATGATCAA 
GAAGATGTGT TCCCATCCAA GCATCGTAAA GGTACTAGAA ATGGTTCATC TTTTTCTCGT 
CATCGAACAA TTCGCGATTT AGACGATGAA GCTGAATCTG TCACCTCTGA GGAAAGTGAG 
TCAGATGATA GTTCTTATGG CGGAACTCCC AAAAAGCGAT CTCGGCAAAA AAAATCAAAC 
ACCTACGTTC AAGATGAAAT TCGATTTTCT TCTAGAAATA GTAAAGGTGT TAACTATAAC 
GAAGACGCGT ATTTTGAATC GTTTGAAGAA GAAGAGGAAG AAGAGATGTA TGAGTACGCA 
ACTGAAGTTT CTGAAGAACC TGAAGATACA CGAGCTATAG ATGTCGTTTT AGATCATAGA 
CTTATTGAAG GACACGATGG CTCTACACCT TCTGAAGATT ATGAGTTTTT AATTAAATGG 
GTTAATTTTT CTCATCTTCA TTGTACATGG GAACCTTACA ATAACATTTC TATGATCAGA 
GGATCTAAAA AGGTCGACAA TCACATTAAG CAAGTCATAC TTCTTGATCG AGAAATTCGA 
GAAGATCCGA CCACTACGCG TGAAGACATT GAAGCAATGG ATATTGAAAA AGAGAGAAAG 
CGTGAAAATT ACGAGGAATA TAAGCAGGTA GACAGAATTG TTGCCAAACA CTTAAACTCC 
GATGGCTCAG TTGAATACTT AGTGAAATGG AAGCAGCTGT TATATGATTT TTGCACATGG 
GAAGCTTCCT CAATTATTGA GCCTATTGCT GCCACGGAAA TTCAGGCATT TCAAGAAAGA 
GAAGAAAGTG CTCTTAGTCC ATCAAGAGGT ACAAACTATG GTAATTCGCG TCCTAAGTAT 
CGAAAACTGG AGCAACAGCC TTCGTATATA ACCGGTGGTG AGCTTCGTGA CTTTCAGCTT 
ACAGGCGTCA ACTGGATGGC ATATCTTTGG CATAAAAATG AAAATGGGAT TTTGGCCGAT 
GAAATGGGTC TCGGTAAGAC AGTCCAAACT GTGGCTTTTC TTTCATATCT TGCACATTCT 
TTAAGACAGC ATGGACCTTT TCTTGTAGTT GTTCCACTTT CTACAGTTCC TGCCTGGCAA 
GAGACATTAG CTCTTTGGGC TTCTGATATG AATTGTATAA GTTACCTTGG AAATACCACC 
TCAAGGCAAG TAATAAGGGA TTACGAATTT TATGTTGATG GAACTCAGAA AATAAAGTTC 
AACTTGCTTC TAACTACTTA TGAGTATGTA CTCAAAGATC GCTCGGTGCT TAGTAATATA 
AAATGGCAAT ATATGGCAAT TGACGAAGCT CATCGATTAA AAAACAGTGA ATCGTCACTT 
TATGAAGCAC TTTCTCAGTT CAAGAACTCA AATCGATTAC TTATAACTGG AACACCATTA 
CAAAATAATA TTCGTGAGCT TGCGGCTTTG GTAGACTTCC TGATGCCAGG AAAGTTTGAG 
ATTCGTGAGG AAATTAATTT AGAAGCACCA GATGAGGAGC AGGAAGCTTA TATTAGAAGT 
TTACAGGAGC ATTTACAACC CTACATTTTA CGAAGATTAA AGAAAGACGT TGAGAAATCT 
CTTCCTTCTA AGTCTGAACG TATCCTTCGT GTTGAGCTTT CTGACTTACA AATGTACTGG 
TACAAAAATA TTTTAACTCG CAATTACCGT GTCCTTACTC AAAGCATAAG TTCGGGAAGT 
CAAATATCTT TACTAAACAT AGTTGTGGAG TTGAAAAAGG CAAGTAATCA TCCTTACTTA 
TTTGACGGAG TAGAAGAGTC ATGGATGCAA AAAATAAACT CTCAAGGAAG ACGAGATGAA 
GTCTTAAAAG GTTTAATTAT GAACAGCGGG AAAATGGTGT TACTTGACAA ACTTCTATCA 
CGTCTTCGTC GAGACGGCCA CCGCGTTTTA ATTTTTAGTC AAATGGTTCG TATGCTTGAC 
ATTCTTGGTG ATTATCTTTC TTTGAGGGGA TATCCACATC AGAGACTGGA TGGCACTGTC 
CCTGCTGCTG TACGAAGAAC TTCAATAGAT CATTTTAATG CACCAAACAG CCCAGATTTT 
GTGTTTTTAT TATCCACACG AGCTGGAGGA TTAGGTATTA ATTTAATGAC TGCTGATACA 
GTTATTATTT TTGATTCCGA TTGGAACCCT CAAGCTGATT TACAAGCTAT GGCACGTGCG 
CATCGCATTG GTCAGAAAAA TCATGTTATG GTATACAGGC TTCTTTCCAA AGATACGATT 
GAAGAAGATG TCTTGGAACG TGCACGTCGT AAAATGATTC TTGAATATGC AATTATTTCA 
TTAGGTGTTA CAGACAAGCA AAAGAATTCC AAAAATGATA AATTTTCTGC TGAAGAGCTT 
AGCGCTATTT TGAAATTTGG AGCTTCTAAT ATGTTTAAAG CAGAGAATAA TCAAAAGAAG 
CTTGAGGATA TGAATTTAGA TGAAATTTTG GAACATGCCG AAGATCATGA TACTTCCAAT 
GATGTGGGTG GAGCCAGCAT GGGTGGTGAA GAGTTTTTGA AACAGTTCGA AGTTACAGAC 
TATAAGGCAG ATGTATCTTG GGATGATATT ATACCCCTAA CTGAACGTGA GAAGTTTGAA 
GAGGAAGACC GCTTAAGAGA AGAGGAAGAA GCGCTTAAGC AAGAAATTGA GCTTAGTAGT 
AGACGAGGTA ATCGTCCTTA TCCTAGCTCT GCGGTGGAAT CCCCATCCTA CTCTGGAACT 
TCTGAAAGGA AGAGCAAAAA GCAAATGCTT AAAGATGAGG TTTTACTGGA AAAGGAAATT 
CGGTTGCTTT ATCGTGCTAT GATTAGATAT GGAAGCCTTG AGCATCGCTA CAATGACATT 
GTTAAATATG CTGATCTTAC TACGCAAGAT GCTCACGTAA TAAAAAAGAT TGCAGCTGAC 
TTAGTAACCG CTTCACGCAA GGCTGTATCA GCAGCCGAGA AGGACTTATC TAATGATCAG 
TCTAATAATA AGTCATCTCG TAAAGCGCTG CTCATTACAT TTAAAGGTGT AAAAAATATA 
AATGCTGAAA CATTGGTACA AAGGCTAAAT GATCTTGATA TTCTTTATGA TGCTATGCCA 
ACGTCAGGAT ATTCTAACTT CCAAATTCCA ATGCACGTTA GATCTGTTCA CGGCTGGTCC 
TGTCAGTGGG GACCCCGAGA AGATTCCATG TTGCTTTCTG GAATTTGTAA GCATGGATTT 
GGAGCTTGGT TAGAAATTCG AGATGATCCA GAACTCAAAA TGAAAGACAA GATCTTTTTA 
GAAGACACAA AACAAACAGA CAACTCTGTC CCCAAAGATA AAGAAAATAA AGAGAAAAAG 
GTACCTTCAG CTGTGCATTT GGTTCGTCGA GGTGAATACC TACTTAGTGC TTTACGTGAG 
CATCACCAAA ATTTTGGCAT AAAATCAAGC CCCGCTATTA GTACTAATGG AAAAACTCAA 
CCTAAGAAGC AGACGGCTAA TCGCAGACAA AGTGGCAAGC CTAACGTTAA ATCTGCGCAA 
AAAATAGAGA GTGCAACTAG GACACCATCG CCTGCAATTT CAGAATCTCG TAAGAAACCT 
TCTTCTAAAG ACACGAAGAT TGAAACACCT AGTAGGGAAC AGTCTCGCAG CCAGACAGCT 
TCACCCGTCA AATCTGAAAA AGACGATGGT AATGTAAGTC TAAATGCTGA ACAAAAGGCA 
CGCTGCAAAG AACTGATGTA TCCAGTCCGT AAGCATATGA AAAGACTTCG GAAAGATTCT 
AGTGGGCTTG GACGCGCGGA GTTGGTGAAG TTACTTACTG AGTGTTTGAC AACGATTGGA 
AAGCATATTG AAAAAACTGT CAATGATACA CCCTCCGAAG AAAAAGCTAC TGTTAGGAAG 
AATTTATGGA TGTTTGCATG TTACTTTTGG CCGAAGGAGG AGGTTAAATA TACCAGCTTG 
ATCAGCATGT ATGAAAAGAT GAAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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hrp3 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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