chd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chd1
Entrez Gene ID	563236
Full Name	chromodomain helicase DNA binding protein 1
Synonyms	si:dkey-106m21.4,wu:fc26h11,wu:fk85d05,zgc:173506
General protein information	Preferred Names chromodomain helicase DNA binding protein 1 Names chromodomain-helicase-DNA-binding protein 1 fc26h11 fk85d05
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001128298.2	NP_001121770.2	chromodomain-helicase-DNA-binding protein 1
XM_009305101.2	XP_009303376.1	chromodomain-helicase-DNA-binding protein 1 isoform X2
XM_009305100.3	XP_009303375.1	chromodomain-helicase-DNA-binding protein 1 isoform X1
XM_009305100.2	XP_009303375.1	chromodomain-helicase-DNA-binding protein 1 isoform X1
XM_009305101.3	XP_009303376.1	chromodomain-helicase-DNA-binding protein 1 isoform X2

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHD1	XP_517850.2
	CHD1	XP_001097125.1
Mus musculus (house mouse)	Chd1	NP_031716.2
Eremothecium gossypii	AGOS_AGR123C	NP_986789.1
Rattus norvegicus (Norway rat)	Chd1	NP_001100935.1
Danio rerio (zebrafish)	zgc:173506	NP_001121770.2
Saccharomyces cerevisiae (baker's yeast)	CHD1	NP_011091.1
Schizosaccharomyces pombe (fission yeast)	hrp3	NP_594967.1
Homo sapiens (human)	CHD1	NP_001261.2
Canis lupus familiaris (dog)	CHD1	XP_848459.1
Gallus gallus (chicken)	CHD1	NP_990272.1
Kluyveromyces lactis	KLLA0C17578g	XP_452985.1
Schizosaccharomyces pombe (fission yeast)	hrp1	NP_593660.1
Bos taurus (cattle)	CHD1	NP_001178977.1

Gene Ontology
Bibliography

Related articles in PubMed

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Asad Z, Pandey A, Babu A, Sun Y, Shevade K, Kapoor S, Ullah I, Ranjan S, Scaria V, Bajpai R, Sachidanandan C
Human molecular genetics25(16)3539-3554(2016 08)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Multiple thyrotropin ?-subunit and thyrotropin receptor-related genes arose during vertebrate evolution.
Maugars G, Dufour S, Cohen-Tannoudji J, Qu?rat B
PloS one9(11)e111361(2014)

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Huang HT, Kathrein KL, Barton A, Gitlin Z, Huang YH, Ward TP, Hofmann O, Dibiase A, Song A, Tyekucheva S, Hide W, Zhou Y, Zon LI
Nature cell biology15(12)1516-25(2013 Dec)

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
Sreenivasan R, Cai M, Bartfai R, Wang X, Christoffels A, Orban L
PloS one3(3)e1791(2008 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa36375	NM_001128298.2 Latest version!	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa15807	XM_009305100.3 Latest version!	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa15808	XM_009305101.3 Latest version!	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
View Old Accession Versions >>
ODa15807	XM_009305100.2	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
ODa15808	XM_009305101.2	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chd1 ( NM_001128298.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001121770.2 Danio rerio chromodomain helicase DNA binding protein 1 (chd1), mRNA.

Price & Availability↑

CloneID	ODa36375
Clone ID Related Accession (Same CDS sequence)	NM_001128298.2
Accession Version	NM_001128298.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5082bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-26
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU302319.9 and BX005434.7. On or before May 30, 2009 this sequence version replaced XM_686602.3, NM_001128298.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGAT GAACTTTAAT GGAAATGAGG GCCGACGCAG CAGGAACAGA 
CGATACTCTG CTTCAGACAG TGATTCAGTC TCTGACCGAA AGAGGCCCAA GAAGCGTGGA 
AGACCTCGAA CTATCCCAAG AGAGAACATT AAAGGCTTCA CTGATGCTGA GATACGAAGA 
TTTATCAAGA GTTACAAAAA GTTTGGTGGC CCTTTAGAGA GACTTGATGC CATCGCTCGT 
GATGCAGAGT TGGTGGATAA ATCGGAGCAT GATCTGCGGC GGCTGGCTGA GACCGTACAC 
AACGGCTGTT TAAGAACGCT AAAGGAGAAC CCTTGTGGAC CTGAGCGAAC CTCAGGCAGG 
CGAGGGAAGG TGAAAGGACC CACTTTCAGG ATCTCAGGCG TTCAAGTCAA TGCTAAGCTG 
GTGATCTCTC ATGAGGAGGA GCTGGCTCCA TTACATAAGG CCATTCCTGC TGACCCCGAG 
GAAAGGAAGA GGTATATAAT CCCATGCCAC TCAAAGGCAG CTCATTTTGA CATTGAGTGG 
GGTAAAGAGG ATGACTCCAG CCTGCTTATT GGCATTTATG AGTATGGGTA TGGCAGCTGG 
GAGATGATCA AGATGGACCC GGACCTCAAC CTCACACATA AGCTCCTTCC TGATGATCCT 
GACAAGAAGC CACAGGCCAA ACAGCTGCAG ACTCGAGCTG ACTACCTCAT CAAATTACTC 
AGCAAAGACC TCGCCAAGAA AGAAGCACAG AAACAGGCTG GCACTGCAAA ATCACGCAAC 
CGGAAACCAC GGGGCAAAAA GAACAAAGCT GTAAAACCGA AGATGGAGGA TGTGACGAAC 
AGCCTGTCCT CTGCGCCGCC TTCAGATAAG AGCTCTGATG AAGAGGATGA GAAGGATGAA 
GAGGACAAGG TTGAGGGACG GGACAGCAAA GAGAGGGAAA AGGCCGATAC CCCTGTTCAC 
ACGCCCACAG GAGGAGACGC AATCCCACTG TCTGAGGAGT CTGAAGAACT GGACCAGAAA 
ACCTTCAGTG TGTGTAAGGA GCGCATGAGG CCTGTGAAGG CTGCACTCAA GCAGTTAGAT 
CGTCCGGAGA AAGGTCTGTC TGAGCGAGAG CAGCTGGAAC ACACGCGGCA ATGCCTGATC 
AAAATAGGAG ATCACATCAC CGAGTGTCTG AGAGAATACA CCAACCCCGA GCAGATCAAA 
CAGTGGAGGA AAAATCTGTG GATATTCGTT TCAAAGTTCA CAGAATTCGA TGCAAGGAAA 
CTGCATAAAC TGTATAAGCA CGCTATTAAG AAACGACAAG AAAATGCTCA GGCAGCAGAG 
CAGAACACTA GCACTGTAAA TACACAAAGC TTTAAACATG CAGATGTTGA GCGGCTAAAG 
GAGTCTCAGC AGGACGAGAG CAGCAGAGAC AGTTACAGCT CTGACAGACA TCATTCATCT 
CGCTACCACG AACACAGCAA AGAGCATCAT GCCAGCGACT CCTACAGGAA GAGCTCCGAC 
TCCAGGAAAA GGCCCTATTC CTCCTTCAGC AACGGCAAAG ACCACAGAGA GCGGGAGCGA 
GACCAGTACA GAGAGAGAGG AGAGCGGCCG GACAGCAGAT ACTACAGTGA GAGCAAGCAC 
AGGAAGCTGG ACGAGCACCG CTCCAGCAGG GAGCACCGAT CAGAAAGCAA CTCCAAGGAC 
AGGACTCACT CGGAGCACCG CTTCCACTCT GAGCACCGCT CCGGCAGCTC CGACTACTCG 
CACCACAAAT CCTCCCGGGA CTATCGCTAC CATTCAGACT GGCAGATGGA GCACCGCTCG 
CCCCGGGACC AGCGCTCGCC TATCGGACAC CGCTCGCCCT TCGAGTACTC GTCCGACCAC 
AAAAGCACAC CGGAACAGGT CTGGAGCGGC CGCAAGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001121770.2
CDS	336..5417
Translation	MDGRSEDESVSNSSGESSNSDDESGSGSGSGSGSSSSSSSSSSSQSGSSDSGSGSDSGSQSDSDSEKSKEKNDQDNKIDGAAFWNSNPSILTVQRTAMLRKQQIQQQQLQQQSSSNTGSDEDSSSSDESDDDSKSRSKGSGSDSDSGSGSGSGSSDSSAEEEANSDESVSDYEPSHKVKSRKPSNKANARNGRNNKAPKKKPSHSSSDDEDDYKKALAGPRRQATVNVSYKEDEELKTDSDDLVEVCGEDVPPPEEDEFETLERVMEMRIGRKGVTGAVTTVYAVEADGDPNANFDPNRQPGEVQYLIKWKNWSHIHNTWETEETLKQQNVKGMKKLDNFKKKEQEKKKWLKAASPEDVEYFNCQQELMDDLHSQYQLVERIIGHSNQKSAAGYPDYLCKWQGLPYSECSWEDGALISKKFQKCIDEYMSRNQCKTIPSRDCKVLKQRPRFVPMKKQPHYIGGEGLELRDYQLDGLNWMAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQLWAPLMNVVVYLGDINSRNMIRTHEWMHPQTKRLKLNILLTTYEILLKDKSFLGNVSWAFIGVDEAHRLKNDDSLLYKTMIEFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFHSWEMFEEEHGKGRDSGYTSLHKELEPFLLRRVKKDVEKSLPAKVEQILRVEMSAVQKQYYKWILTRNYKALSKGTKGSTSGFLNIMMELKKCCNHCYLIKPPDDNEFYNRQEGLQHLVRSSGKLILLDKLLVRLKERGHRVLIFSQMVRMLDILAEYLKYRQFLFQRLDGSIKGEMRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEEIIERAKKKMVLDHLVIQRMDTTGKTVLHTGAAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETRENDPGPSTVGEELLSQFKVANFSMMEDEEIDMDTERNDRNWDDIIPEDHRRRLEEEERQKELAEIYLLPRMRKCAKQMNFNGNEGRRSRNRRYSASDSDSVSDRKRPKKRGRPRTIPRENIKGFTDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSEHDLRRLAETVHNGCLRTLKENPCGPERTSGRRGKVKGPTFRISGVQVNAKLVISHEEELAPLHKAIPADPEERKRYIIPCHSKAAHFDIEWGKEDDSSLLIGIYEYGYGSWEMIKMDPDLNLTHKLLPDDPDKKPQAKQLQTRADYLIKLLSKDLAKKEAQKQAGTAKSRNRKPRGKKNKAVKPKMEDVTNSLSSAPPSDKSSDEEDEKDEEDKVEGRDSKEREKADTPVHTPTGGDAIPLSEESEELDQKTFSVCKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLREYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQENAQAAEQNTSTVNTQSFKHADVERLKESQQDESSRDSYSSDRHHSSRYHEHSKEHHASDSYRKSSDSRKRPYSSFSNGKDHRERERDQYRERGERPDSRYYSESKHRKLDEHRSSREHRSESNSKDRTHSEHRFHSEHRSGSSDYSHHKSSRDYRYHSDWQMEHRSPRDQRSPIGHRSPFEYSSDHKSTPEQVWSGRKT

Target ORF information:

RefSeq Version	NM_001128298.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001128298.2

ORF Insert Sequence:

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGAT GAACTTTAAT GGAAATGAGG GCCGACGCAG CAGGAACAGA 
CGATACTCTG CTTCAGACAG TGATTCAGTC TCTGACCGAA AGAGGCCCAA GAAGCGTGGA 
AGACCTCGAA CTATCCCAAG AGAGAACATT AAAGGCTTCA CTGATGCTGA GATACGAAGA 
TTTATCAAGA GTTACAAAAA GTTTGGTGGC CCTTTAGAGA GACTTGATGC CATCGCTCGT 
GATGCAGAGT TGGTGGATAA ATCGGAGCAT GATCTGCGGC GGCTGGCTGA GACCGTACAC 
AACGGCTGTT TAAGAACGCT AAAGGAGAAC CCTTGTGGAC CTGAGCGAAC CTCAGGCAGG 
CGAGGGAAGG TGAAAGGACC CACTTTCAGG ATCTCAGGCG TTCAAGTCAA TGCTAAGCTG 
GTGATCTCTC ATGAGGAGGA GCTGGCTCCA TTACATAAGG CCATTCCTGC TGACCCCGAG 
GAAAGGAAGA GGTATATAAT CCCATGCCAC TCAAAGGCAG CTCATTTTGA CATTGAGTGG 
GGTAAAGAGG ATGACTCCAG CCTGCTTATT GGCATTTATG AGTATGGGTA TGGCAGCTGG 
GAGATGATCA AGATGGACCC GGACCTCAAC CTCACACATA AGCTCCTTCC TGATGATCCT 
GACAAGAAGC CACAGGCCAA ACAGCTGCAG ACTCGAGCTG ACTACCTCAT CAAATTACTC 
AGCAAAGACC TCGCCAAGAA AGAAGCACAG AAACAGGCTG GCACTGCAAA ATCACGCAAC 
CGGAAACCAC GGGGCAAAAA GAACAAAGCT GTAAAACCGA AGATGGAGGA TGTGACGAAC 
AGCCTGTCCT CTGCGCCGCC TTCAGATAAG AGCTCTGATG AAGAGGATGA GAAGGATGAA 
GAGGACAAGG TTGAGGGACG GGACAGCAAA GAGAGGGAAA AGGCCGATAC CCCTGTTCAC 
ACGCCCACAG GAGGAGACGC AATCCCACTG TCTGAGGAGT CTGAAGAACT GGACCAGAAA 
ACCTTCAGTG TGTGTAAGGA GCGCATGAGG CCTGTGAAGG CTGCACTCAA GCAGTTAGAT 
CGTCCGGAGA AAGGTCTGTC TGAGCGAGAG CAGCTGGAAC ACACGCGGCA ATGCCTGATC 
AAAATAGGAG ATCACATCAC CGAGTGTCTG AGAGAATACA CCAACCCCGA GCAGATCAAA 
CAGTGGAGGA AAAATCTGTG GATATTCGTT TCAAAGTTCA CAGAATTCGA TGCAAGGAAA 
CTGCATAAAC TGTATAAGCA CGCTATTAAG AAACGACAAG AAAATGCTCA GGCAGCAGAG 
CAGAACACTA GCACTGTAAA TACACAAAGC TTTAAACATG CAGATGTTGA GCGGCTAAAG 
GAGTCTCAGC AGGACGAGAG CAGCAGAGAC AGTTACAGCT CTGACAGACA TCATTCATCT 
CGCTACCACG AACACAGCAA AGAGCATCAT GCCAGCGACT CCTACAGGAA GAGCTCCGAC 
TCCAGGAAAA GGCCCTATTC CTCCTTCAGC AACGGCAAAG ACCACAGAGA GCGGGAGCGA 
GACCAGTACA GAGAGAGAGG AGAGCGGCCG GACAGCAGAT ACTACAGTGA GAGCAAGCAC 
AGGAAGCTGG ACGAGCACCG CTCCAGCAGG GAGCACCGAT CAGAAAGCAA CTCCAAGGAC 
AGGACTCACT CGGAGCACCG CTTCCACTCT GAGCACCGCT CCGGCAGCTC CGACTACTCG 
CACCACAAAT CCTCCCGGGA CTATCGCTAC CATTCAGACT GGCAGATGGA GCACCGCTCG 
CCCCGGGACC AGCGCTCGCC TATCGGACAC CGCTCGCCCT TCGAGTACTC GTCCGACCAC 
AAAAGCACAC CGGAACAGGT CTGGAGCGGC CGCAAGACAT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd1 ( XM_009305100.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303375.1 Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X1, mRNA.

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CloneID	ODa15807
Clone ID Related Accession (Same CDS sequence)	XM_009305100.3 , XM_009305100.2
Accession Version	XM_009305100.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305100.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGCA GATGAACTTT AATGGAAATG AGGGCCGACG CAGCAGGAAC 
AGACGATACT CTGCTTCAGA CAGTGATTCA GTCTCTGACC GAAAGAGGCC CAAGAAGCGT 
GGAAGACCTC GAACTATCCC AAGAGAGAAC ATTAAAGGCT TCACTGATGC TGAGATACGA 
AGATTTATCA AGAGTTACAA AAAGTTTGGT GGCCCTTTAG AGAGACTTGA TGCCATCGCT 
CGTGATGCAG AGTTGGTGGA TAAATCGGAG CATGATCTGC GGCGGCTGGC TGAGACCGTA 
CACAACGGCT GTTTAAGAAC GCTAAAGGAG AACCCTTGTG GACCTGAGCG AACCTCAGGA 
GGCAGGCGAG GGAAGGTGAA AGGACCCACT TTCAGGATCT CAGGCGTTCA AGTCAATGCT 
AAGCTGGTGA TCTCTCATGA GGAGGAGCTG GCTCCATTAC ATAAGGCCAT TCCTGCTGAC 
CCCGAGGAAA GGAAGAGGTA TATAATCCCA TGCCACTCAA AGGCAGCTCA TTTTGACATT 
GAGTGGGGTA AAGAGGATGA CTCCAGCCTG CTTATTGGCA TTTATGAGTA TGGGTATGGC 
AGCTGGGAGA TGATCAAGAT GGACCCGGAC CTCAACCTCA CACATAAGCT CCTTCCTGAT 
GATCCTGACA AGAAGCCACA GGCCAAACAG CTGCAGACTC GAGCTGACTA CCTCATCAAA 
TTACTCAGCA AAGACCTCGC CAAGAAAGAA GCACAGAAAC AGGCTGGCAC TGCAAAATCA 
CGCAACCGGA AACCACGGGG CAAAAAGAAC AAAGCTGTAA AACCGAAGAT GGAGGATGTG 
ACGAACAGCC TGTCCTCTGC GCCGCCTTCA GATAAGAGCT CTGATGAAGA GGATGAGAAG 
GATGAAGAGG ACAAGGAAAC TCCTCCTCCC GTTAAGCTGC CGAACAGAAG AGCACGTGTA 
CCAGAGCGGA CCGTAATGGA AGAAGAAGAT GACTCTGATG AGGATGAGCT TGAGGACGCA 
GCGCCAGTAG AGAAGGACAC ACCAGAGAAA GTTGTTAAAA AGGAAGTTAA GAAGGAGAAG 
AAAGAAGAGG GACGGGACAG CAAAGAGAGG GAAGTGAAGG AGAAGGAAAT CAAACCAGAG 
CCTTTGCAAA GAGAGGATGA TTCTCCAGCA GAGGCTGTCG ATATAAAAGC AGAGAAGGCC 
GATACCCCTG TTCACACGCC CACAGGAGGA GACGCAATCC CACTGTCTGA GGAGTCTGAA 
GAACTGGACC AGAAAACCTT CAGTGTGTGT AAGGAGCGCA TGAGGCCTGT GAAGGCTGCA 
CTCAAGCAGT TAGATCGTCC GGAGAAAGGT CTGTCTGAGC GAGAGCAGCT GGAACACACG 
CGGCAATGCC TGATCAAAAT AGGAGATCAC ATCACCGAGT GTCTGAGAGA ATACACCAAC 
CCCGAGCAGA TCAAACAGTG GAGGAAAAAT CTGTGGATAT TCGTTTCAAA GTTCACAGAA 
TTCGATGCAA GGAAACTGCA TAAACTGTAT AAGCACGCTA TTAAGAAACG ACAAGAAAAT 
GCTCAGGCAG CAGAGCAGAA CACTAGCACT GTAAATACAC AAAGCTTTAA ACATGCAGAT 
GTTGAGCGGC TAAAGGAGTC TCAGCAGGAC GAGAGCAGCA GAGACAGTTA CAGCTCTGAC 
AGACATCATT CATCTCGCTA CCACGAACAC AGCAAAGAGC ATCATGCCAG CGACTCCTAC 
AGGAAGAGCT CCGACTCCAG GAAAAGGCCC TATTCCTCCT TCAGCAACGG CAAAGACCAC 
AGAGAGCGGG AGCGAGACCA GTACAGAGAG AGAGGAGAGC GGCCGGACAG CAGATACTAC 
AGTGAGAGCA AGCACAGGAA GCTGGACGAG CACCGCTCCA GCAGGGAGCA CCGATCAGAA 
AGCAACTCCA AGGACAGGAC TCACTCGGAG CACCGCTTCC ACTCTGAGCA CCGCTCCGGC 
AGCTCCGACT ACTCGCACCA CAAATCCTCC CGGGACTATC GCTACCATTC AGACTGGCAG 
ATGGAGCACC GCTCGCCCCG GGACCAGCGC TCGCCTATCG GACACCGCTC GCCCTTCGAG 
TACTCGTCCG ACCACAAAAG CACACCGGAA CAGGTCTGGA GCGGCCGCAA GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303375.1
CDS	339..5675
Translation	MDGRSEDESVSNSSGESSNSDDESGSGSGSGSGSSSSSSSSSSSQSGSSDSGSGSDSGSQSDSDSEKSKEKNDQDNKIDGAAFWNSNPSILTVQRTAMLRKQQIQQQQLQQQSSSNTGSDEDSSSSDESDDDSKSRSKGSGSDSDSGSGSGSGSSDSSAEEEANSDESVSDYEPSHKVKSRKPSNKANARNGRNNKAPKKKPSHSSSDDEDDYKKALAGPRRQATVNVSYKEDEELKTDSDDLVEVCGEDVPPPEEDEFETLERVMEMRIGRKGVTGAVTTVYAVEADGDPNANFDPNRQPGEVQYLIKWKNWSHIHNTWETEETLKQQNVKGMKKLDNFKKKEQEKKKWLKAASPEDVEYFNCQQELMDDLHSQYQLVERIIGHSNQKSAAGYPDYLCKWQGLPYSECSWEDGALISKKFQKCIDEYMSRNQCKTIPSRDCKVLKQRPRFVPMKKQPHYIGGEGLELRDYQLDGLNWMAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQLWAPLMNVVVYLGDINSRNMIRTHEWMHPQTKRLKLNILLTTYEILLKDKSFLGNVSWAFIGVDEAHRLKNDDSLLYKTMIEFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFHSWEMFEEEHGKGRDSGYTSLHKELEPFLLRRVKKDVEKSLPAKVEQILRVEMSAVQKQYYKWILTRNYKALSKGTKGSTSGFLNIMMELKKCCNHCYLIKPPDDNEFYNRQEGLQHLVRSSGKLILLDKLLVRLKERGHRVLIFSQMVRMLDILAEYLKYRQFLFQRLDGSIKGEMRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEEIIERAKKKMVLDHLVIQRMDTTGKTVLHTGAAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETRENDPGPSTVGEELLSQFKVANFSMMEDEEIDMDTERNDRNWDDIIPEDHRRRLEEEERQKELAEIYLLPRMRKCAKQQMNFNGNEGRRSRNRRYSASDSDSVSDRKRPKKRGRPRTIPRENIKGFTDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSEHDLRRLAETVHNGCLRTLKENPCGPERTSGGRRGKVKGPTFRISGVQVNAKLVISHEEELAPLHKAIPADPEERKRYIIPCHSKAAHFDIEWGKEDDSSLLIGIYEYGYGSWEMIKMDPDLNLTHKLLPDDPDKKPQAKQLQTRADYLIKLLSKDLAKKEAQKQAGTAKSRNRKPRGKKNKAVKPKMEDVTNSLSSAPPSDKSSDEEDEKDEEDKETPPPVKLPNRRARVPERTVMEEEDDSDEDELEDAAPVEKDTPEKVVKKEVKKEKKEEGRDSKEREVKEKEIKPEPLQREDDSPAEAVDIKAEKADTPVHTPTGGDAIPLSEESEELDQKTFSVCKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLREYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQENAQAAEQNTSTVNTQSFKHADVERLKESQQDESSRDSYSSDRHHSSRYHEHSKEHHASDSYRKSSDSRKRPYSSFSNGKDHRERERDQYRERGERPDSRYYSESKHRKLDEHRSSREHRSESNSKDRTHSEHRFHSEHRSGSSDYSHHKSSRDYRYHSDWQMEHRSPRDQRSPIGHRSPFEYSSDHKSTPEQVWSGRKT

Target ORF information:

RefSeq Version	XM_009305100.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305100.3

ORF Insert Sequence:

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGCA GATGAACTTT AATGGAAATG AGGGCCGACG CAGCAGGAAC 
AGACGATACT CTGCTTCAGA CAGTGATTCA GTCTCTGACC GAAAGAGGCC CAAGAAGCGT 
GGAAGACCTC GAACTATCCC AAGAGAGAAC ATTAAAGGCT TCACTGATGC TGAGATACGA 
AGATTTATCA AGAGTTACAA AAAGTTTGGT GGCCCTTTAG AGAGACTTGA TGCCATCGCT 
CGTGATGCAG AGTTGGTGGA TAAATCGGAG CATGATCTGC GGCGGCTGGC TGAGACCGTA 
CACAACGGCT GTTTAAGAAC GCTAAAGGAG AACCCTTGTG GACCTGAGCG AACCTCAGGA 
GGCAGGCGAG GGAAGGTGAA AGGACCCACT TTCAGGATCT CAGGCGTTCA AGTCAATGCT 
AAGCTGGTGA TCTCTCATGA GGAGGAGCTG GCTCCATTAC ATAAGGCCAT TCCTGCTGAC 
CCCGAGGAAA GGAAGAGGTA TATAATCCCA TGCCACTCAA AGGCAGCTCA TTTTGACATT 
GAGTGGGGTA AAGAGGATGA CTCCAGCCTG CTTATTGGCA TTTATGAGTA TGGGTATGGC 
AGCTGGGAGA TGATCAAGAT GGACCCGGAC CTCAACCTCA CACATAAGCT CCTTCCTGAT 
GATCCTGACA AGAAGCCACA GGCCAAACAG CTGCAGACTC GAGCTGACTA CCTCATCAAA 
TTACTCAGCA AAGACCTCGC CAAGAAAGAA GCACAGAAAC AGGCTGGCAC TGCAAAATCA 
CGCAACCGGA AACCACGGGG CAAAAAGAAC AAAGCTGTAA AACCGAAGAT GGAGGATGTG 
ACGAACAGCC TGTCCTCTGC GCCGCCTTCA GATAAGAGCT CTGATGAAGA GGATGAGAAG 
GATGAAGAGG ACAAGGAAAC TCCTCCTCCC GTTAAGCTGC CGAACAGAAG AGCACGTGTA 
CCAGAGCGGA CCGTAATGGA AGAAGAAGAT GACTCTGATG AGGATGAGCT TGAGGACGCA 
GCGCCAGTAG AGAAGGACAC ACCAGAGAAA GTTGTTAAAA AGGAAGTTAA GAAGGAGAAG 
AAAGAAGAGG GACGGGACAG CAAAGAGAGG GAAGTGAAGG AGAAGGAAAT CAAACCAGAG 
CCTTTGCAAA GAGAGGATGA TTCTCCAGCA GAGGCTGTCG ATATAAAAGC AGAGAAGGCC 
GATACCCCTG TTCACACGCC CACAGGAGGA GACGCAATCC CACTGTCTGA GGAGTCTGAA 
GAACTGGACC AGAAAACCTT CAGTGTGTGT AAGGAGCGCA TGAGGCCTGT GAAGGCTGCA 
CTCAAGCAGT TAGATCGTCC GGAGAAAGGT CTGTCTGAGC GAGAGCAGCT GGAACACACG 
CGGCAATGCC TGATCAAAAT AGGAGATCAC ATCACCGAGT GTCTGAGAGA ATACACCAAC 
CCCGAGCAGA TCAAACAGTG GAGGAAAAAT CTGTGGATAT TCGTTTCAAA GTTCACAGAA 
TTCGATGCAA GGAAACTGCA TAAACTGTAT AAGCACGCTA TTAAGAAACG ACAAGAAAAT 
GCTCAGGCAG CAGAGCAGAA CACTAGCACT GTAAATACAC AAAGCTTTAA ACATGCAGAT 
GTTGAGCGGC TAAAGGAGTC TCAGCAGGAC GAGAGCAGCA GAGACAGTTA CAGCTCTGAC 
AGACATCATT CATCTCGCTA CCACGAACAC AGCAAAGAGC ATCATGCCAG CGACTCCTAC 
AGGAAGAGCT CCGACTCCAG GAAAAGGCCC TATTCCTCCT TCAGCAACGG CAAAGACCAC 
AGAGAGCGGG AGCGAGACCA GTACAGAGAG AGAGGAGAGC GGCCGGACAG CAGATACTAC 
AGTGAGAGCA AGCACAGGAA GCTGGACGAG CACCGCTCCA GCAGGGAGCA CCGATCAGAA 
AGCAACTCCA AGGACAGGAC TCACTCGGAG CACCGCTTCC ACTCTGAGCA CCGCTCCGGC 
AGCTCCGACT ACTCGCACCA CAAATCCTCC CGGGACTATC GCTACCATTC AGACTGGCAG 
ATGGAGCACC GCTCGCCCCG GGACCAGCGC TCGCCTATCG GACACCGCTC GCCCTTCGAG 
TACTCGTCCG ACCACAAAAG CACACCGGAA CAGGTCTGGA GCGGCCGCAA GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd1 ( XM_009305100.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303375.1 Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15807
Clone ID Related Accession (Same CDS sequence)	XM_009305100.3 , XM_009305100.2
Accession Version	XM_009305100.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5337bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chromodomain-helicase-DNA-binding protein 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305100.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGCA GATGAACTTT AATGGAAATG AGGGCCGACG CAGCAGGAAC 
AGACGATACT CTGCTTCAGA CAGTGATTCA GTCTCTGACC GAAAGAGGCC CAAGAAGCGT 
GGAAGACCTC GAACTATCCC AAGAGAGAAC ATTAAAGGCT TCACTGATGC TGAGATACGA 
AGATTTATCA AGAGTTACAA AAAGTTTGGT GGCCCTTTAG AGAGACTTGA TGCCATCGCT 
CGTGATGCAG AGTTGGTGGA TAAATCGGAG CATGATCTGC GGCGGCTGGC TGAGACCGTA 
CACAACGGCT GTTTAAGAAC GCTAAAGGAG AACCCTTGTG GACCTGAGCG AACCTCAGGA 
GGCAGGCGAG GGAAGGTGAA AGGACCCACT TTCAGGATCT CAGGCGTTCA AGTCAATGCT 
AAGCTGGTGA TCTCTCATGA GGAGGAGCTG GCTCCATTAC ATAAGGCCAT TCCTGCTGAC 
CCCGAGGAAA GGAAGAGGTA TATAATCCCA TGCCACTCAA AGGCAGCTCA TTTTGACATT 
GAGTGGGGTA AAGAGGATGA CTCCAGCCTG CTTATTGGCA TTTATGAGTA TGGGTATGGC 
AGCTGGGAGA TGATCAAGAT GGACCCGGAC CTCAACCTCA CACATAAGCT CCTTCCTGAT 
GATCCTGACA AGAAGCCACA GGCCAAACAG CTGCAGACTC GAGCTGACTA CCTCATCAAA 
TTACTCAGCA AAGACCTCGC CAAGAAAGAA GCACAGAAAC AGGCTGGCAC TGCAAAATCA 
CGCAACCGGA AACCACGGGG CAAAAAGAAC AAAGCTGTAA AACCGAAGAT GGAGGATGTG 
ACGAACAGCC TGTCCTCTGC GCCGCCTTCA GATAAGAGCT CTGATGAAGA GGATGAGAAG 
GATGAAGAGG ACAAGGAAAC TCCTCCTCCC GTTAAGCTGC CGAACAGAAG AGCACGTGTA 
CCAGAGCGGA CCGTAATGGA AGAAGAAGAT GACTCTGATG AGGATGAGCT TGAGGACGCA 
GCGCCAGTAG AGAAGGACAC ACCAGAGAAA GTTGTTAAAA AGGAAGTTAA GAAGGAGAAG 
AAAGAAGAGG GACGGGACAG CAAAGAGAGG GAAGTGAAGG AGAAGGAAAT CAAACCAGAG 
CCTTTGCAAA GAGAGGATGA TTCTCCAGCA GAGGCTGTCG ATATAAAAGC AGAGAAGGCC 
GATACCCCTG TTCACACGCC CACAGGAGGA GACGCAATCC CACTGTCTGA GGAGTCTGAA 
GAACTGGACC AGAAAACCTT CAGTGTGTGT AAGGAGCGCA TGAGGCCTGT GAAGGCTGCA 
CTCAAGCAGT TAGATCGTCC GGAGAAAGGT CTGTCTGAGC GAGAGCAGCT GGAACACACG 
CGGCAATGCC TGATCAAAAT AGGAGATCAC ATCACCGAGT GTCTGAGAGA ATACACCAAC 
CCCGAGCAGA TCAAACAGTG GAGGAAAAAT CTGTGGATAT TCGTTTCAAA GTTCACAGAA 
TTCGATGCAA GGAAACTGCA TAAACTGTAT AAGCACGCTA TTAAGAAACG ACAAGAAAAT 
GCTCAGGCAG CAGAGCAGAA CACTAGCACT GTAAATACAC AAAGCTTTAA ACATGCAGAT 
GTTGAGCGGC TAAAGGAGTC TCAGCAGGAC GAGAGCAGCA GAGACAGTTA CAGCTCTGAC 
AGACATCATT CATCTCGCTA CCACGAACAC AGCAAAGAGC ATCATGCCAG CGACTCCTAC 
AGGAAGAGCT CCGACTCCAG GAAAAGGCCC TATTCCTCCT TCAGCAACGG CAAAGACCAC 
AGAGAGCGGG AGCGAGACCA GTACAGAGAG AGAGGAGAGC GGCCGGACAG CAGATACTAC 
AGTGAGAGCA AGCACAGGAA GCTGGACGAG CACCGCTCCA GCAGGGAGCA CCGATCAGAA 
AGCAACTCCA AGGACAGGAC TCACTCGGAG CACCGCTTCC ACTCTGAGCA CCGCTCCGGC 
AGCTCCGACT ACTCGCACCA CAAATCCTCC CGGGACTATC GCTACCATTC AGACTGGCAG 
ATGGAGCACC GCTCGCCCCG GGACCAGCGC TCGCCTATCG GACACCGCTC GCCCTTCGAG 
TACTCGTCCG ACCACAAAAG CACACCGGAA CAGGTCTGGA GCGGCCGCAA GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303375.1
CDS	338..5674
Translation	MDGRSEDESVSNSSGESSNSDDESGSGSGSGSGSSSSSSSSSSSQSGSSDSGSGSDSGSQSDSDSEKSKEKNDQDNKIDGAAFWNSNPSILTVQRTAMLRKQQIQQQQLQQQSSSNTGSDEDSSSSDESDDDSKSRSKGSGSDSDSGSGSGSGSSDSSAEEEANSDESVSDYEPSHKVKSRKPSNKANARNGRNNKAPKKKPSHSSSDDEDDYKKALAGPRRQATVNVSYKEDEELKTDSDDLVEVCGEDVPPPEEDEFETLERVMEMRIGRKGVTGAVTTVYAVEADGDPNANFDPNRQPGEVQYLIKWKNWSHIHNTWETEETLKQQNVKGMKKLDNFKKKEQEKKKWLKAASPEDVEYFNCQQELMDDLHSQYQLVERIIGHSNQKSAAGYPDYLCKWQGLPYSECSWEDGALISKKFQKCIDEYMSRNQCKTIPSRDCKVLKQRPRFVPMKKQPHYIGGEGLELRDYQLDGLNWMAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQLWAPLMNVVVYLGDINSRNMIRTHEWMHPQTKRLKLNILLTTYEILLKDKSFLGNVSWAFIGVDEAHRLKNDDSLLYKTMIEFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFHSWEMFEEEHGKGRDSGYTSLHKELEPFLLRRVKKDVEKSLPAKVEQILRVEMSAVQKQYYKWILTRNYKALSKGTKGSTSGFLNIMMELKKCCNHCYLIKPPDDNEFYNRQEGLQHLVRSSGKLILLDKLLVRLKERGHRVLIFSQMVRMLDILAEYLKYRQFLFQRLDGSIKGEMRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEEIIERAKKKMVLDHLVIQRMDTTGKTVLHTGAAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETRENDPGPSTVGEELLSQFKVANFSMMEDEEIDMDTERNDRNWDDIIPEDHRRRLEEEERQKELAEIYLLPRMRKCAKQQMNFNGNEGRRSRNRRYSASDSDSVSDRKRPKKRGRPRTIPRENIKGFTDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSEHDLRRLAETVHNGCLRTLKENPCGPERTSGGRRGKVKGPTFRISGVQVNAKLVISHEEELAPLHKAIPADPEERKRYIIPCHSKAAHFDIEWGKEDDSSLLIGIYEYGYGSWEMIKMDPDLNLTHKLLPDDPDKKPQAKQLQTRADYLIKLLSKDLAKKEAQKQAGTAKSRNRKPRGKKNKAVKPKMEDVTNSLSSAPPSDKSSDEEDEKDEEDKETPPPVKLPNRRARVPERTVMEEEDDSDEDELEDAAPVEKDTPEKVVKKEVKKEKKEEGRDSKEREVKEKEIKPEPLQREDDSPAEAVDIKAEKADTPVHTPTGGDAIPLSEESEELDQKTFSVCKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLREYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQENAQAAEQNTSTVNTQSFKHADVERLKESQQDESSRDSYSSDRHHSSRYHEHSKEHHASDSYRKSSDSRKRPYSSFSNGKDHRERERDQYRERGERPDSRYYSESKHRKLDEHRSSREHRSESNSKDRTHSEHRFHSEHRSGSSDYSHHKSSRDYRYHSDWQMEHRSPRDQRSPIGHRSPFEYSSDHKSTPEQVWSGRKT

Target ORF information:

RefSeq Version	XM_009305100.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305100.2

ORF Insert Sequence:

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGCA GATGAACTTT AATGGAAATG AGGGCCGACG CAGCAGGAAC 
AGACGATACT CTGCTTCAGA CAGTGATTCA GTCTCTGACC GAAAGAGGCC CAAGAAGCGT 
GGAAGACCTC GAACTATCCC AAGAGAGAAC ATTAAAGGCT TCACTGATGC TGAGATACGA 
AGATTTATCA AGAGTTACAA AAAGTTTGGT GGCCCTTTAG AGAGACTTGA TGCCATCGCT 
CGTGATGCAG AGTTGGTGGA TAAATCGGAG CATGATCTGC GGCGGCTGGC TGAGACCGTA 
CACAACGGCT GTTTAAGAAC GCTAAAGGAG AACCCTTGTG GACCTGAGCG AACCTCAGGA 
GGCAGGCGAG GGAAGGTGAA AGGACCCACT TTCAGGATCT CAGGCGTTCA AGTCAATGCT 
AAGCTGGTGA TCTCTCATGA GGAGGAGCTG GCTCCATTAC ATAAGGCCAT TCCTGCTGAC 
CCCGAGGAAA GGAAGAGGTA TATAATCCCA TGCCACTCAA AGGCAGCTCA TTTTGACATT 
GAGTGGGGTA AAGAGGATGA CTCCAGCCTG CTTATTGGCA TTTATGAGTA TGGGTATGGC 
AGCTGGGAGA TGATCAAGAT GGACCCGGAC CTCAACCTCA CACATAAGCT CCTTCCTGAT 
GATCCTGACA AGAAGCCACA GGCCAAACAG CTGCAGACTC GAGCTGACTA CCTCATCAAA 
TTACTCAGCA AAGACCTCGC CAAGAAAGAA GCACAGAAAC AGGCTGGCAC TGCAAAATCA 
CGCAACCGGA AACCACGGGG CAAAAAGAAC AAAGCTGTAA AACCGAAGAT GGAGGATGTG 
ACGAACAGCC TGTCCTCTGC GCCGCCTTCA GATAAGAGCT CTGATGAAGA GGATGAGAAG 
GATGAAGAGG ACAAGGAAAC TCCTCCTCCC GTTAAGCTGC CGAACAGAAG AGCACGTGTA 
CCAGAGCGGA CCGTAATGGA AGAAGAAGAT GACTCTGATG AGGATGAGCT TGAGGACGCA 
GCGCCAGTAG AGAAGGACAC ACCAGAGAAA GTTGTTAAAA AGGAAGTTAA GAAGGAGAAG 
AAAGAAGAGG GACGGGACAG CAAAGAGAGG GAAGTGAAGG AGAAGGAAAT CAAACCAGAG 
CCTTTGCAAA GAGAGGATGA TTCTCCAGCA GAGGCTGTCG ATATAAAAGC AGAGAAGGCC 
GATACCCCTG TTCACACGCC CACAGGAGGA GACGCAATCC CACTGTCTGA GGAGTCTGAA 
GAACTGGACC AGAAAACCTT CAGTGTGTGT AAGGAGCGCA TGAGGCCTGT GAAGGCTGCA 
CTCAAGCAGT TAGATCGTCC GGAGAAAGGT CTGTCTGAGC GAGAGCAGCT GGAACACACG 
CGGCAATGCC TGATCAAAAT AGGAGATCAC ATCACCGAGT GTCTGAGAGA ATACACCAAC 
CCCGAGCAGA TCAAACAGTG GAGGAAAAAT CTGTGGATAT TCGTTTCAAA GTTCACAGAA 
TTCGATGCAA GGAAACTGCA TAAACTGTAT AAGCACGCTA TTAAGAAACG ACAAGAAAAT 
GCTCAGGCAG CAGAGCAGAA CACTAGCACT GTAAATACAC AAAGCTTTAA ACATGCAGAT 
GTTGAGCGGC TAAAGGAGTC TCAGCAGGAC GAGAGCAGCA GAGACAGTTA CAGCTCTGAC 
AGACATCATT CATCTCGCTA CCACGAACAC AGCAAAGAGC ATCATGCCAG CGACTCCTAC 
AGGAAGAGCT CCGACTCCAG GAAAAGGCCC TATTCCTCCT TCAGCAACGG CAAAGACCAC 
AGAGAGCGGG AGCGAGACCA GTACAGAGAG AGAGGAGAGC GGCCGGACAG CAGATACTAC 
AGTGAGAGCA AGCACAGGAA GCTGGACGAG CACCGCTCCA GCAGGGAGCA CCGATCAGAA 
AGCAACTCCA AGGACAGGAC TCACTCGGAG CACCGCTTCC ACTCTGAGCA CCGCTCCGGC 
AGCTCCGACT ACTCGCACCA CAAATCCTCC CGGGACTATC GCTACCATTC AGACTGGCAG 
ATGGAGCACC GCTCGCCCCG GGACCAGCGC TCGCCTATCG GACACCGCTC GCCCTTCGAG 
TACTCGTCCG ACCACAAAAG CACACCGGAA CAGGTCTGGA GCGGCCGCAA GACATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd1 ( XM_009305101.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303376.1 Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X2, mRNA.

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CloneID	ODa15808
Clone ID Related Accession (Same CDS sequence)	XM_009305101.2 , XM_009305101.3
Accession Version	XM_009305101.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	chromodomain-helicase-DNA-binding protein 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009305101.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGAT GAACTTTAAT GGAAATGAGG GCCGACGCAG CAGGAACAGA 
CGATACTCTG CTTCAGACAG TGATTCAGTC TCTGACCGAA AGAGGCCCAA GAAGCGTGGA 
AGACCTCGAA CTATCCCAAG AGAGAACATT AAAGGCTTCA CTGATGCTGA GATACGAAGA 
TTTATCAAGA GTTACAAAAA GTTTGGTGGC CCTTTAGAGA GACTTGATGC CATCGCTCGT 
GATGCAGAGT TGGTGGATAA ATCGGAGCAT GATCTGCGGC GGCTGGCTGA GACCGTACAC 
AACGGCTGTT TAAGAACGCT AAAGGAGAAC CCTTGTGGAC CTGAGCGAAC CTCAGGAGGC 
AGGCGAGGGA AGGTGAAAGG ACCCACTTTC AGGATCTCAG GCGTTCAAGT CAATGCTAAG 
CTGGTGATCT CTCATGAGGA GGAGCTGGCT CCATTACATA AGGCCATTCC TGCTGACCCC 
GAGGAAAGGA AGAGGTATAT AATCCCATGC CACTCAAAGG CAGCTCATTT TGACATTGAG 
TGGGGTAAAG AGGATGACTC CAGCCTGCTT ATTGGCATTT ATGAGTATGG GTATGGCAGC 
TGGGAGATGA TCAAGATGGA CCCGGACCTC AACCTCACAC ATAAGCTCCT TCCTGATGAT 
CCTGACAAGA AGCCACAGGC CAAACAGCTG CAGACTCGAG CTGACTACCT CATCAAATTA 
CTCAGCAAAG ACCTCGCCAA GAAAGAAGCA CAGAAACAGG CTGGCACTGC AAAATCACGC 
AACCGGAAAC CACGGGGCAA AAAGAACAAA GCTGTAAAAC CGAAGATGGA GGATGTGACG 
AACAGCCTGT CCTCTGCGCC GCCTTCAGAT AAGAGCTCTG ATGAAGAGGA TGAGAAGGAT 
GAAGAGGACA AGGAAACTCC TCCTCCCGTT AAGCTGCCGA ACAGAAGAGC ACGTGTACCA 
GAGCGGACCG TAATGGAAGA AGAAGATGAC TCTGATGAGG ATGAGCTTGA GGACGCAGCG 
CCAGTAGAGA AGGACACACC AGAGAAAGTT GTTAAAAAGG AAGTTAAGAA GGAGAAGAAA 
GAAGAGGGAC GGGACAGCAA AGAGAGGGAA GTGAAGGAGA AGGAAATCAA ACCAGAGCCT 
TTGCAAAGAG AGGATGATTC TCCAGCAGAG GCTGTCGATA TAAAAGCAGA GAAGGCCGAT 
ACCCCTGTTC ACACGCCCAC AGGAGGAGAC GCAATCCCAC TGTCTGAGGA GTCTGAAGAA 
CTGGACCAGA AAACCTTCAG TGTGTGTAAG GAGCGCATGA GGCCTGTGAA GGCTGCACTC 
AAGCAGTTAG ATCGTCCGGA GAAAGGTCTG TCTGAGCGAG AGCAGCTGGA ACACACGCGG 
CAATGCCTGA TCAAAATAGG AGATCACATC ACCGAGTGTC TGAGAGAATA CACCAACCCC 
GAGCAGATCA AACAGTGGAG GAAAAATCTG TGGATATTCG TTTCAAAGTT CACAGAATTC 
GATGCAAGGA AACTGCATAA ACTGTATAAG CACGCTATTA AGAAACGACA AGAAAATGCT 
CAGGCAGCAG AGCAGAACAC TAGCACTGTA AATACACAAA GCTTTAAACA TGCAGATGTT 
GAGCGGCTAA AGGAGTCTCA GCAGGACGAG AGCAGCAGAG ACAGTTACAG CTCTGACAGA 
CATCATTCAT CTCGCTACCA CGAACACAGC AAAGAGCATC ATGCCAGCGA CTCCTACAGG 
AAGAGCTCCG ACTCCAGGAA AAGGCCCTAT TCCTCCTTCA GCAACGGCAA AGACCACAGA 
GAGCGGGAGC GAGACCAGTA CAGAGAGAGA GGAGAGCGGC CGGACAGCAG ATACTACAGT 
GAGAGCAAGC ACAGGAAGCT GGACGAGCAC CGCTCCAGCA GGGAGCACCG ATCAGAAAGC 
AACTCCAAGG ACAGGACTCA CTCGGAGCAC CGCTTCCACT CTGAGCACCG CTCCGGCAGC 
TCCGACTACT CGCACCACAA ATCCTCCCGG GACTATCGCT ACCATTCAGA CTGGCAGATG 
GAGCACCGCT CGCCCCGGGA CCAGCGCTCG CCTATCGGAC ACCGCTCGCC CTTCGAGTAC 
TCGTCCGACC ACAAAAGCAC ACCGGAACAG GTCTGGAGCG GCCGCAAGAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303376.1
CDS	336..5669
Translation	MDGRSEDESVSNSSGESSNSDDESGSGSGSGSGSSSSSSSSSSSQSGSSDSGSGSDSGSQSDSDSEKSKEKNDQDNKIDGAAFWNSNPSILTVQRTAMLRKQQIQQQQLQQQSSSNTGSDEDSSSSDESDDDSKSRSKGSGSDSDSGSGSGSGSSDSSAEEEANSDESVSDYEPSHKVKSRKPSNKANARNGRNNKAPKKKPSHSSSDDEDDYKKALAGPRRQATVNVSYKEDEELKTDSDDLVEVCGEDVPPPEEDEFETLERVMEMRIGRKGVTGAVTTVYAVEADGDPNANFDPNRQPGEVQYLIKWKNWSHIHNTWETEETLKQQNVKGMKKLDNFKKKEQEKKKWLKAASPEDVEYFNCQQELMDDLHSQYQLVERIIGHSNQKSAAGYPDYLCKWQGLPYSECSWEDGALISKKFQKCIDEYMSRNQCKTIPSRDCKVLKQRPRFVPMKKQPHYIGGEGLELRDYQLDGLNWMAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQLWAPLMNVVVYLGDINSRNMIRTHEWMHPQTKRLKLNILLTTYEILLKDKSFLGNVSWAFIGVDEAHRLKNDDSLLYKTMIEFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFHSWEMFEEEHGKGRDSGYTSLHKELEPFLLRRVKKDVEKSLPAKVEQILRVEMSAVQKQYYKWILTRNYKALSKGTKGSTSGFLNIMMELKKCCNHCYLIKPPDDNEFYNRQEGLQHLVRSSGKLILLDKLLVRLKERGHRVLIFSQMVRMLDILAEYLKYRQFLFQRLDGSIKGEMRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEEIIERAKKKMVLDHLVIQRMDTTGKTVLHTGAAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETRENDPGPSTVGEELLSQFKVANFSMMEDEEIDMDTERNDRNWDDIIPEDHRRRLEEEERQKELAEIYLLPRMRKCAKQMNFNGNEGRRSRNRRYSASDSDSVSDRKRPKKRGRPRTIPRENIKGFTDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSEHDLRRLAETVHNGCLRTLKENPCGPERTSGGRRGKVKGPTFRISGVQVNAKLVISHEEELAPLHKAIPADPEERKRYIIPCHSKAAHFDIEWGKEDDSSLLIGIYEYGYGSWEMIKMDPDLNLTHKLLPDDPDKKPQAKQLQTRADYLIKLLSKDLAKKEAQKQAGTAKSRNRKPRGKKNKAVKPKMEDVTNSLSSAPPSDKSSDEEDEKDEEDKETPPPVKLPNRRARVPERTVMEEEDDSDEDELEDAAPVEKDTPEKVVKKEVKKEKKEEGRDSKEREVKEKEIKPEPLQREDDSPAEAVDIKAEKADTPVHTPTGGDAIPLSEESEELDQKTFSVCKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLREYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQENAQAAEQNTSTVNTQSFKHADVERLKESQQDESSRDSYSSDRHHSSRYHEHSKEHHASDSYRKSSDSRKRPYSSFSNGKDHRERERDQYRERGERPDSRYYSESKHRKLDEHRSSREHRSESNSKDRTHSEHRFHSEHRSGSSDYSHHKSSRDYRYHSDWQMEHRSPRDQRSPIGHRSPFEYSSDHKSTPEQVWSGRKT

Target ORF information:

RefSeq Version	XM_009305101.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305101.2

ORF Insert Sequence:

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGAT GAACTTTAAT GGAAATGAGG GCCGACGCAG CAGGAACAGA 
CGATACTCTG CTTCAGACAG TGATTCAGTC TCTGACCGAA AGAGGCCCAA GAAGCGTGGA 
AGACCTCGAA CTATCCCAAG AGAGAACATT AAAGGCTTCA CTGATGCTGA GATACGAAGA 
TTTATCAAGA GTTACAAAAA GTTTGGTGGC CCTTTAGAGA GACTTGATGC CATCGCTCGT 
GATGCAGAGT TGGTGGATAA ATCGGAGCAT GATCTGCGGC GGCTGGCTGA GACCGTACAC 
AACGGCTGTT TAAGAACGCT AAAGGAGAAC CCTTGTGGAC CTGAGCGAAC CTCAGGAGGC 
AGGCGAGGGA AGGTGAAAGG ACCCACTTTC AGGATCTCAG GCGTTCAAGT CAATGCTAAG 
CTGGTGATCT CTCATGAGGA GGAGCTGGCT CCATTACATA AGGCCATTCC TGCTGACCCC 
GAGGAAAGGA AGAGGTATAT AATCCCATGC CACTCAAAGG CAGCTCATTT TGACATTGAG 
TGGGGTAAAG AGGATGACTC CAGCCTGCTT ATTGGCATTT ATGAGTATGG GTATGGCAGC 
TGGGAGATGA TCAAGATGGA CCCGGACCTC AACCTCACAC ATAAGCTCCT TCCTGATGAT 
CCTGACAAGA AGCCACAGGC CAAACAGCTG CAGACTCGAG CTGACTACCT CATCAAATTA 
CTCAGCAAAG ACCTCGCCAA GAAAGAAGCA CAGAAACAGG CTGGCACTGC AAAATCACGC 
AACCGGAAAC CACGGGGCAA AAAGAACAAA GCTGTAAAAC CGAAGATGGA GGATGTGACG 
AACAGCCTGT CCTCTGCGCC GCCTTCAGAT AAGAGCTCTG ATGAAGAGGA TGAGAAGGAT 
GAAGAGGACA AGGAAACTCC TCCTCCCGTT AAGCTGCCGA ACAGAAGAGC ACGTGTACCA 
GAGCGGACCG TAATGGAAGA AGAAGATGAC TCTGATGAGG ATGAGCTTGA GGACGCAGCG 
CCAGTAGAGA AGGACACACC AGAGAAAGTT GTTAAAAAGG AAGTTAAGAA GGAGAAGAAA 
GAAGAGGGAC GGGACAGCAA AGAGAGGGAA GTGAAGGAGA AGGAAATCAA ACCAGAGCCT 
TTGCAAAGAG AGGATGATTC TCCAGCAGAG GCTGTCGATA TAAAAGCAGA GAAGGCCGAT 
ACCCCTGTTC ACACGCCCAC AGGAGGAGAC GCAATCCCAC TGTCTGAGGA GTCTGAAGAA 
CTGGACCAGA AAACCTTCAG TGTGTGTAAG GAGCGCATGA GGCCTGTGAA GGCTGCACTC 
AAGCAGTTAG ATCGTCCGGA GAAAGGTCTG TCTGAGCGAG AGCAGCTGGA ACACACGCGG 
CAATGCCTGA TCAAAATAGG AGATCACATC ACCGAGTGTC TGAGAGAATA CACCAACCCC 
GAGCAGATCA AACAGTGGAG GAAAAATCTG TGGATATTCG TTTCAAAGTT CACAGAATTC 
GATGCAAGGA AACTGCATAA ACTGTATAAG CACGCTATTA AGAAACGACA AGAAAATGCT 
CAGGCAGCAG AGCAGAACAC TAGCACTGTA AATACACAAA GCTTTAAACA TGCAGATGTT 
GAGCGGCTAA AGGAGTCTCA GCAGGACGAG AGCAGCAGAG ACAGTTACAG CTCTGACAGA 
CATCATTCAT CTCGCTACCA CGAACACAGC AAAGAGCATC ATGCCAGCGA CTCCTACAGG 
AAGAGCTCCG ACTCCAGGAA AAGGCCCTAT TCCTCCTTCA GCAACGGCAA AGACCACAGA 
GAGCGGGAGC GAGACCAGTA CAGAGAGAGA GGAGAGCGGC CGGACAGCAG ATACTACAGT 
GAGAGCAAGC ACAGGAAGCT GGACGAGCAC CGCTCCAGCA GGGAGCACCG ATCAGAAAGC 
AACTCCAAGG ACAGGACTCA CTCGGAGCAC CGCTTCCACT CTGAGCACCG CTCCGGCAGC 
TCCGACTACT CGCACCACAA ATCCTCCCGG GACTATCGCT ACCATTCAGA CTGGCAGATG 
GAGCACCGCT CGCCCCGGGA CCAGCGCTCG CCTATCGGAC ACCGCTCGCC CTTCGAGTAC 
TCGTCCGACC ACAAAAGCAC ACCGGAACAG GTCTGGAGCG GCCGCAAGAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

chd1 ( XM_009305101.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009303376.1 Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X2, mRNA.

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CloneID	ODa15808
Clone ID Related Accession (Same CDS sequence)	XM_009305101.2 , XM_009305101.3
Accession Version	XM_009305101.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5334bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	chromodomain-helicase-DNA-binding protein 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009305101.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGAT GAACTTTAAT GGAAATGAGG GCCGACGCAG CAGGAACAGA 
CGATACTCTG CTTCAGACAG TGATTCAGTC TCTGACCGAA AGAGGCCCAA GAAGCGTGGA 
AGACCTCGAA CTATCCCAAG AGAGAACATT AAAGGCTTCA CTGATGCTGA GATACGAAGA 
TTTATCAAGA GTTACAAAAA GTTTGGTGGC CCTTTAGAGA GACTTGATGC CATCGCTCGT 
GATGCAGAGT TGGTGGATAA ATCGGAGCAT GATCTGCGGC GGCTGGCTGA GACCGTACAC 
AACGGCTGTT TAAGAACGCT AAAGGAGAAC CCTTGTGGAC CTGAGCGAAC CTCAGGAGGC 
AGGCGAGGGA AGGTGAAAGG ACCCACTTTC AGGATCTCAG GCGTTCAAGT CAATGCTAAG 
CTGGTGATCT CTCATGAGGA GGAGCTGGCT CCATTACATA AGGCCATTCC TGCTGACCCC 
GAGGAAAGGA AGAGGTATAT AATCCCATGC CACTCAAAGG CAGCTCATTT TGACATTGAG 
TGGGGTAAAG AGGATGACTC CAGCCTGCTT ATTGGCATTT ATGAGTATGG GTATGGCAGC 
TGGGAGATGA TCAAGATGGA CCCGGACCTC AACCTCACAC ATAAGCTCCT TCCTGATGAT 
CCTGACAAGA AGCCACAGGC CAAACAGCTG CAGACTCGAG CTGACTACCT CATCAAATTA 
CTCAGCAAAG ACCTCGCCAA GAAAGAAGCA CAGAAACAGG CTGGCACTGC AAAATCACGC 
AACCGGAAAC CACGGGGCAA AAAGAACAAA GCTGTAAAAC CGAAGATGGA GGATGTGACG 
AACAGCCTGT CCTCTGCGCC GCCTTCAGAT AAGAGCTCTG ATGAAGAGGA TGAGAAGGAT 
GAAGAGGACA AGGAAACTCC TCCTCCCGTT AAGCTGCCGA ACAGAAGAGC ACGTGTACCA 
GAGCGGACCG TAATGGAAGA AGAAGATGAC TCTGATGAGG ATGAGCTTGA GGACGCAGCG 
CCAGTAGAGA AGGACACACC AGAGAAAGTT GTTAAAAAGG AAGTTAAGAA GGAGAAGAAA 
GAAGAGGGAC GGGACAGCAA AGAGAGGGAA GTGAAGGAGA AGGAAATCAA ACCAGAGCCT 
TTGCAAAGAG AGGATGATTC TCCAGCAGAG GCTGTCGATA TAAAAGCAGA GAAGGCCGAT 
ACCCCTGTTC ACACGCCCAC AGGAGGAGAC GCAATCCCAC TGTCTGAGGA GTCTGAAGAA 
CTGGACCAGA AAACCTTCAG TGTGTGTAAG GAGCGCATGA GGCCTGTGAA GGCTGCACTC 
AAGCAGTTAG ATCGTCCGGA GAAAGGTCTG TCTGAGCGAG AGCAGCTGGA ACACACGCGG 
CAATGCCTGA TCAAAATAGG AGATCACATC ACCGAGTGTC TGAGAGAATA CACCAACCCC 
GAGCAGATCA AACAGTGGAG GAAAAATCTG TGGATATTCG TTTCAAAGTT CACAGAATTC 
GATGCAAGGA AACTGCATAA ACTGTATAAG CACGCTATTA AGAAACGACA AGAAAATGCT 
CAGGCAGCAG AGCAGAACAC TAGCACTGTA AATACACAAA GCTTTAAACA TGCAGATGTT 
GAGCGGCTAA AGGAGTCTCA GCAGGACGAG AGCAGCAGAG ACAGTTACAG CTCTGACAGA 
CATCATTCAT CTCGCTACCA CGAACACAGC AAAGAGCATC ATGCCAGCGA CTCCTACAGG 
AAGAGCTCCG ACTCCAGGAA AAGGCCCTAT TCCTCCTTCA GCAACGGCAA AGACCACAGA 
GAGCGGGAGC GAGACCAGTA CAGAGAGAGA GGAGAGCGGC CGGACAGCAG ATACTACAGT 
GAGAGCAAGC ACAGGAAGCT GGACGAGCAC CGCTCCAGCA GGGAGCACCG ATCAGAAAGC 
AACTCCAAGG ACAGGACTCA CTCGGAGCAC CGCTTCCACT CTGAGCACCG CTCCGGCAGC 
TCCGACTACT CGCACCACAA ATCCTCCCGG GACTATCGCT ACCATTCAGA CTGGCAGATG 
GAGCACCGCT CGCCCCGGGA CCAGCGCTCG CCTATCGGAC ACCGCTCGCC CTTCGAGTAC 
TCGTCCGACC ACAAAAGCAC ACCGGAACAG GTCTGGAGCG GCCGCAAGAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009303376.1
CDS	336..5669
Translation	MDGRSEDESVSNSSGESSNSDDESGSGSGSGSGSSSSSSSSSSSQSGSSDSGSGSDSGSQSDSDSEKSKEKNDQDNKIDGAAFWNSNPSILTVQRTAMLRKQQIQQQQLQQQSSSNTGSDEDSSSSDESDDDSKSRSKGSGSDSDSGSGSGSGSSDSSAEEEANSDESVSDYEPSHKVKSRKPSNKANARNGRNNKAPKKKPSHSSSDDEDDYKKALAGPRRQATVNVSYKEDEELKTDSDDLVEVCGEDVPPPEEDEFETLERVMEMRIGRKGVTGAVTTVYAVEADGDPNANFDPNRQPGEVQYLIKWKNWSHIHNTWETEETLKQQNVKGMKKLDNFKKKEQEKKKWLKAASPEDVEYFNCQQELMDDLHSQYQLVERIIGHSNQKSAAGYPDYLCKWQGLPYSECSWEDGALISKKFQKCIDEYMSRNQCKTIPSRDCKVLKQRPRFVPMKKQPHYIGGEGLELRDYQLDGLNWMAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQLWAPLMNVVVYLGDINSRNMIRTHEWMHPQTKRLKLNILLTTYEILLKDKSFLGNVSWAFIGVDEAHRLKNDDSLLYKTMIEFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFHSWEMFEEEHGKGRDSGYTSLHKELEPFLLRRVKKDVEKSLPAKVEQILRVEMSAVQKQYYKWILTRNYKALSKGTKGSTSGFLNIMMELKKCCNHCYLIKPPDDNEFYNRQEGLQHLVRSSGKLILLDKLLVRLKERGHRVLIFSQMVRMLDILAEYLKYRQFLFQRLDGSIKGEMRKQALDHFNADGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEEIIERAKKKMVLDHLVIQRMDTTGKTVLHTGAAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETRENDPGPSTVGEELLSQFKVANFSMMEDEEIDMDTERNDRNWDDIIPEDHRRRLEEEERQKELAEIYLLPRMRKCAKQMNFNGNEGRRSRNRRYSASDSDSVSDRKRPKKRGRPRTIPRENIKGFTDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSEHDLRRLAETVHNGCLRTLKENPCGPERTSGGRRGKVKGPTFRISGVQVNAKLVISHEEELAPLHKAIPADPEERKRYIIPCHSKAAHFDIEWGKEDDSSLLIGIYEYGYGSWEMIKMDPDLNLTHKLLPDDPDKKPQAKQLQTRADYLIKLLSKDLAKKEAQKQAGTAKSRNRKPRGKKNKAVKPKMEDVTNSLSSAPPSDKSSDEEDEKDEEDKETPPPVKLPNRRARVPERTVMEEEDDSDEDELEDAAPVEKDTPEKVVKKEVKKEKKEEGRDSKEREVKEKEIKPEPLQREDDSPAEAVDIKAEKADTPVHTPTGGDAIPLSEESEELDQKTFSVCKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLREYTNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQENAQAAEQNTSTVNTQSFKHADVERLKESQQDESSRDSYSSDRHHSSRYHEHSKEHHASDSYRKSSDSRKRPYSSFSNGKDHRERERDQYRERGERPDSRYYSESKHRKLDEHRSSREHRSESNSKDRTHSEHRFHSEHRSGSSDYSHHKSSRDYRYHSDWQMEHRSPRDQRSPIGHRSPFEYSSDHKSTPEQVWSGRKT

Target ORF information:

RefSeq Version	XM_009305101.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chromodomain helicase DNA binding protein 1 (chd1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009305101.3

ORF Insert Sequence:

ATGGATGGAC GCAGCGAGGA TGAAAGTGTC AGCAACAGCA GCGGAGAGTC CAGTAATTCA 
GATGATGAGT CCGGGTCAGG ATCAGGTTCT GGCTCTGGCT CGAGTTCAAG CAGTAGCAGT 
TCTAGCAGCA GCCAATCAGG GAGCAGCGAC TCAGGAAGTG GCTCAGATTC TGGAAGCCAG 
TCAGACTCGG ACTCGGAGAA ATCCAAGGAA AAGAACGATC AAGACAACAA AATCGATGGA 
GCAGCGTTTT GGAATTCCAA CCCGAGTATC CTGACAGTGC AAAGGACGGC CATGCTCAGG 
AAGCAGCAGA TCCAACAGCA GCAGCTCCAG CAGCAGAGCT CCTCAAACAC CGGCTCAGAT 
GAGGACTCTT CCAGTAGTGA TGAGTCAGAT GATGACTCAA AGAGCAGGAG TAAAGGGTCT 
GGATCAGACT CTGACTCAGG CTCGGGTTCA GGAAGTGGTT CCTCAGACTC TTCAGCAGAG 
GAGGAAGCGA ATAGTGACGA GTCGGTGTCA GACTATGAGC CCAGTCACAA AGTCAAGAGC 
AGAAAACCTT CAAACAAGGC CAACGCCAGA AATGGGAGAA ATAACAAAGC TCCCAAGAAG 
AAGCCCTCCC ACTCTTCCTC AGATGATGAA GATGACTATA AAAAAGCTTT GGCAGGGCCC 
CGACGGCAGG CCACGGTCAA CGTAAGCTAC AAAGAAGATG AGGAGCTGAA GACCGATTCA 
GATGACCTGG TGGAGGTGTG CGGAGAGGAT GTCCCGCCAC CAGAGGAGGA TGAGTTTGAG 
ACACTGGAGA GAGTCATGGA AATGAGGATA GGCAGGAAAG GAGTAACCGG GGCTGTGACC 
ACAGTATATG CTGTAGAGGC AGATGGTGAT CCCAACGCCA ACTTCGACCC CAACAGACAG 
CCAGGAGAGG TGCAATATTT GATCAAATGG AAGAACTGGT CTCACATTCA CAACACTTGG 
GAGACGGAGG AGACACTCAA ACAACAGAAT GTGAAGGGCA TGAAGAAACT GGACAACTTT 
AAGAAAAAAG AGCAGGAGAA AAAGAAATGG CTCAAAGCTG CCTCACCTGA GGACGTGGAG 
TACTTCAACT GTCAGCAGGA GCTCATGGAT GACTTGCATT CTCAATATCA ACTTGTGGAG 
AGAATTATTG GACATTCAAA TCAGAAGTCT GCCGCAGGCT ATCCAGATTA CCTTTGTAAA 
TGGCAGGGGT TGCCATACTC TGAGTGCAGC TGGGAAGACG GTGCTCTTAT TTCTAAAAAG 
TTTCAAAAAT GCATTGATGA ATACATGAGC CGGAACCAGT GCAAAACCAT CCCCTCCAGA 
GACTGCAAGG TTTTAAAACA GAGGCCACGG TTTGTTCCCA TGAAGAAGCA ACCTCATTAC 
ATTGGTGGAG AAGGTTTGGA GCTGCGGGAC TATCAGCTGG ATGGTCTGAA CTGGATGGCC 
CATTCTTGGT GCAAAGGAAA CAGTTGTATC CTGGCAGACG AGATGGGTCT GGGCAAAACC 
ATCCAGACCA TCAGTTTCCT CAATTACTTG TTCCATGAGC ACCAGCTTTA CGGGCCGTTC 
CTTCTGGTGG TGCCACTGTC CACCCTCACA TCCTGGCAGA GAGAGATACA GCTCTGGGCT 
CCTCTGATGA ATGTTGTGGT GTACCTGGGA GATATCAACA GCAGAAACAT GATTCGAACA 
CATGAATGGA TGCATCCGCA GACCAAACGA CTGAAGTTGA ACATTCTTCT GACTACATAT 
GAAATTCTGC TGAAGGATAA GTCATTTTTA GGAAATGTAA GCTGGGCGTT CATTGGTGTA 
GACGAGGCCC ATCGGCTGAA GAACGATGAC TCTCTGCTCT ACAAGACTAT GATAGAGTTC 
AAGTCCAATC ACAGGCTCCT GATCACTGGC ACACCCCTCC AGAACTCCCT CAAAGAGCTC 
TGGTCTCTGC TACACTTCAT CATGCCTGAA AAGTTTCACT CGTGGGAGAT GTTTGAAGAG 
GAACATGGGA AAGGCAGGGA TTCGGGCTAC ACAAGCCTTC ATAAGGAGCT GGAGCCATTC 
CTGCTGCGCA GAGTAAAGAA GGATGTGGAG AAATCTCTGC CTGCCAAGGT GGAACAGATC 
CTCCGGGTGG AGATGAGCGC GGTTCAGAAA CAATACTACA AGTGGATATT GACGAGAAAC 
TATAAAGCCC TGAGTAAAGG CACTAAAGGA AGCACGTCAG GCTTTCTCAA CATCATGATG 
GAGTTGAAGA AATGCTGTAA CCACTGCTAC CTCATTAAAC CGCCTGACGA CAACGAGTTC 
TACAACAGAC AAGAAGGCCT ACAGCACCTT GTTCGAAGTA GTGGGAAGCT GATCCTTCTG 
GATAAACTGC TGGTTCGGCT GAAGGAACGA GGGCACCGTG TGCTCATCTT CTCGCAGATG 
GTTCGGATGC TGGACATCCT GGCTGAATAT CTGAAGTACC GGCAGTTCTT ATTCCAGAGA 
CTGGATGGTT CCATAAAGGG GGAAATGAGA AAGCAGGCTT TGGATCATTT TAATGCAGAC 
GGCTCTGAGG ATTTCTGTTT CCTGTTGTCC ACACGAGCGG GTGGTCTGGG CATTAACCTG 
GCCTCTGCAG ATACAGTGGT TATTTTTGAC TCAGACTGGA ACCCTCAGAA TGACCTGCAG 
GCCCAGGCCA GAGCTCACAG GATTGGTCAG AAAAAACAGG TGAATATATA CCGTCTGGTG 
ACCAAGGGCT CAGTTGAGGA GGAAATTATT GAGCGAGCCA AGAAGAAAAT GGTGCTGGAC 
CATCTCGTCA TTCAGAGGAT GGACACCACG GGAAAGACTG TGCTTCATAC GGGTGCTGCT 
CCTTCCAGTT CTACTCCATT CAACAAGGAG GAACTGTCAG CTATATTGAA GTTTGGTGCT 
GAAGAACTTT TCAAAGAGCC AGAAGGAGAG GAACAGGAAC CTCAGGAGAT GGATATCGAT 
GAAATCCTGA AGAGAGCAGA AACCAGAGAG AATGACCCAG GACCATCGAC TGTAGGAGAA 
GAGCTGCTCT CACAGTTTAA GGTGGCAAAT TTCTCCATGA TGGAAGATGA GGAAATTGAC 
ATGGACACAG AGAGGAATGA CAGGAACTGG GATGACATTA TCCCAGAGGA TCATCGGAGG 
AGACTCGAAG AGGAGGAGCG ACAGAAAGAG CTCGCAGAAA TCTATTTACT GCCCCGAATG 
AGGAAATGCG CTAAACAGAT GAACTTTAAT GGAAATGAGG GCCGACGCAG CAGGAACAGA 
CGATACTCTG CTTCAGACAG TGATTCAGTC TCTGACCGAA AGAGGCCCAA GAAGCGTGGA 
AGACCTCGAA CTATCCCAAG AGAGAACATT AAAGGCTTCA CTGATGCTGA GATACGAAGA 
TTTATCAAGA GTTACAAAAA GTTTGGTGGC CCTTTAGAGA GACTTGATGC CATCGCTCGT 
GATGCAGAGT TGGTGGATAA ATCGGAGCAT GATCTGCGGC GGCTGGCTGA GACCGTACAC 
AACGGCTGTT TAAGAACGCT AAAGGAGAAC CCTTGTGGAC CTGAGCGAAC CTCAGGAGGC 
AGGCGAGGGA AGGTGAAAGG ACCCACTTTC AGGATCTCAG GCGTTCAAGT CAATGCTAAG 
CTGGTGATCT CTCATGAGGA GGAGCTGGCT CCATTACATA AGGCCATTCC TGCTGACCCC 
GAGGAAAGGA AGAGGTATAT AATCCCATGC CACTCAAAGG CAGCTCATTT TGACATTGAG 
TGGGGTAAAG AGGATGACTC CAGCCTGCTT ATTGGCATTT ATGAGTATGG GTATGGCAGC 
TGGGAGATGA TCAAGATGGA CCCGGACCTC AACCTCACAC ATAAGCTCCT TCCTGATGAT 
CCTGACAAGA AGCCACAGGC CAAACAGCTG CAGACTCGAG CTGACTACCT CATCAAATTA 
CTCAGCAAAG ACCTCGCCAA GAAAGAAGCA CAGAAACAGG CTGGCACTGC AAAATCACGC 
AACCGGAAAC CACGGGGCAA AAAGAACAAA GCTGTAAAAC CGAAGATGGA GGATGTGACG 
AACAGCCTGT CCTCTGCGCC GCCTTCAGAT AAGAGCTCTG ATGAAGAGGA TGAGAAGGAT 
GAAGAGGACA AGGAAACTCC TCCTCCCGTT AAGCTGCCGA ACAGAAGAGC ACGTGTACCA 
GAGCGGACCG TAATGGAAGA AGAAGATGAC TCTGATGAGG ATGAGCTTGA GGACGCAGCG 
CCAGTAGAGA AGGACACACC AGAGAAAGTT GTTAAAAAGG AAGTTAAGAA GGAGAAGAAA 
GAAGAGGGAC GGGACAGCAA AGAGAGGGAA GTGAAGGAGA AGGAAATCAA ACCAGAGCCT 
TTGCAAAGAG AGGATGATTC TCCAGCAGAG GCTGTCGATA TAAAAGCAGA GAAGGCCGAT 
ACCCCTGTTC ACACGCCCAC AGGAGGAGAC GCAATCCCAC TGTCTGAGGA GTCTGAAGAA 
CTGGACCAGA AAACCTTCAG TGTGTGTAAG GAGCGCATGA GGCCTGTGAA GGCTGCACTC 
AAGCAGTTAG ATCGTCCGGA GAAAGGTCTG TCTGAGCGAG AGCAGCTGGA ACACACGCGG 
CAATGCCTGA TCAAAATAGG AGATCACATC ACCGAGTGTC TGAGAGAATA CACCAACCCC 
GAGCAGATCA AACAGTGGAG GAAAAATCTG TGGATATTCG TTTCAAAGTT CACAGAATTC 
GATGCAAGGA AACTGCATAA ACTGTATAAG CACGCTATTA AGAAACGACA AGAAAATGCT 
CAGGCAGCAG AGCAGAACAC TAGCACTGTA AATACACAAA GCTTTAAACA TGCAGATGTT 
GAGCGGCTAA AGGAGTCTCA GCAGGACGAG AGCAGCAGAG ACAGTTACAG CTCTGACAGA 
CATCATTCAT CTCGCTACCA CGAACACAGC AAAGAGCATC ATGCCAGCGA CTCCTACAGG 
AAGAGCTCCG ACTCCAGGAA AAGGCCCTAT TCCTCCTTCA GCAACGGCAA AGACCACAGA 
GAGCGGGAGC GAGACCAGTA CAGAGAGAGA GGAGAGCGGC CGGACAGCAG ATACTACAGT 
GAGAGCAAGC ACAGGAAGCT GGACGAGCAC CGCTCCAGCA GGGAGCACCG ATCAGAAAGC 
AACTCCAAGG ACAGGACTCA CTCGGAGCAC CGCTTCCACT CTGAGCACCG CTCCGGCAGC 
TCCGACTACT CGCACCACAA ATCCTCCCGG GACTATCGCT ACCATTCAGA CTGGCAGATG 
GAGCACCGCT CGCCCCGGGA CCAGCGCTCG CCTATCGGAC ACCGCTCGCC CTTCGAGTAC 
TCGTCCGACC ACAAAAGCAC ACCGGAACAG GTCTGGAGCG GCCGCAAGAC ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Rescue of neural crest-derived phenotypes in a zebrafish CHARGE model by Sox10 downregulation.
Human molecular genetics25(16)3539-3554(2016 08)
Asad Z,Pandey A,Babu A,Sun Y,Shevade K,Kapoor S,Ullah I,Ranjan S,Scaria V,Bajpai R,Sachidanandan C

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Multiple thyrotropin ?-subunit and thyrotropin receptor-related genes arose during vertebrate evolution.
PloS one9(11)e111361(2014)
Maugars G,Dufour S,Cohen-Tannoudji J,Qu?rat B

A network of epigenetic regulators guides developmental haematopoiesis in vivo.
Nature cell biology15(12)1516-25(2013 Dec)
Huang HT,Kathrein KL,Barton A,Gitlin Z,Huang YH,Ward TP,Hofmann O,Dibiase A,Song A,Tyekucheva S,Hide W,Zhou Y,Zon LI

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
PloS one3(3)e1791(2008 Mar)
Sreenivasan R,Cai M,Bartfai R,Wang X,Christoffels A,Orban L

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chd1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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