Chd1 cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Chd1
Entrez Gene ID	12648
Full Name	chromodomain helicase DNA binding protein 1
Synonyms	4930525N21Rik,AI851787,AW555109
General protein information	Preferred Names chromodomain helicase DNA binding protein 1 Names chromodomain-helicase-DNA-binding protein 1 ATP-dependent helicase CHD1 CHD-1
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 17 Map Location: 17 A2\|17 8.95 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_007690.3	NP_031716.2	chromodomain-helicase-DNA-binding protein 1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CHD1	XP_517850.2
	CHD1	XP_001097125.1
Mus musculus (house mouse)	Chd1	NP_031716.2
Eremothecium gossypii	AGOS_AGR123C	NP_986789.1
Rattus norvegicus (Norway rat)	Chd1	NP_001100935.1
Danio rerio (zebrafish)	zgc:173506	NP_001121770.2
Saccharomyces cerevisiae (baker's yeast)	CHD1	NP_011091.1
Schizosaccharomyces pombe (fission yeast)	hrp3	NP_594967.1
Homo sapiens (human)	CHD1	NP_001261.2
Canis lupus familiaris (dog)	CHD1	XP_848459.1
Gallus gallus (chicken)	CHD1	NP_990272.1
Kluyveromyces lactis	KLLA0C17578g	XP_452985.1
Schizosaccharomyces pombe (fission yeast)	hrp1	NP_593660.1
Bos taurus (cattle)	CHD1	NP_001178977.1

Gene Ontology
Bibliography

Related articles in PubMed

CHD1 loss sensitizes prostate cancer to DNA damaging therapy by promoting error-prone double-strand break repair.
Shenoy TR, Boysen G, Wang MY, Xu QZ, Guo W, Koh FM, Wang C, Zhang LZ, Wang Y, Gil V, Aziz S, Christova R, Rodrigues DN, Crespo M, Rescigno P, Tunariu N, Riisnaes R, Zafeiriou Z, Flohr P, Yuan W, Knight E, Swain A, Ramalho-Santos M, Xu DY, de Bono J, Wu H
Annals of oncology : official journal of the European Society for Medical Oncology28(7)1495-1507(2017 Jul)

High-throughput discovery of novel developmental phenotypes.
Dickinson ME, Flenniken AM, Ji X, Teboul L, Wong MD, White JK, Meehan TF, Weninger WJ, Westerberg H, Adissu H, Baker CN, Bower L, Brown JM, Caddle LB, Chiani F, Clary D, Cleak J, Daly MJ, Denegre JM, Doe B, Dolan ME, Edie SM, Fuchs H, Gailus-Durner V, Galli A, Gambadoro A, Gallegos J, Guo S, Horner NR, Hsu CW, Johnson SJ, Kalaga S, Keith LC, Lanoue L, Lawson TN, Lek M, Mark M, Marschall S, Mason J, McElwee ML, Newbigging S, Nutter LM, Peterson KA, Ramirez-Solis R, Rowland DJ, Ryder E, Samocha KE, Seavitt JR, Selloum M, Szoke-Kovacs Z, Tamura M, Trainor AG, Tudose I, Wakana S, Warren J, Wendling O, West DB, Wong L, Yoshiki A, , , , , , , , , MacArthur DG, Tocchini-Valentini GP, Gao X, Flicek P, Bradley A, Skarnes WC, Justice MJ, Parkinson HE, Moore M, Wells S, Braun RE, Svenson KL, de Angelis MH, Herault Y, Mohun T, Mallon AM, Henkelman RM, Brown SD, Adams DJ, Lloyd KC, McKerlie C, Beaudet AL, Bu?an M, Murray SA
Nature537(7621)508-514(2016 09)

CHD1 acts via the Hmgpi pathway to regulate mouse early embryogenesis.
Suzuki S, Nozawa Y, Tsukamoto S, Kaneko T, Manabe I, Imai H, Minami N
Development (Cambridge, England)142(13)2375-84(2015 Jul)

Emergence of hematopoietic stem and progenitor cells involves a Chd1-dependent increase in total nascent transcription.
Koh FM, Lizama CO, Wong P, Hawkins JS, Zovein AC, Ramalho-Santos M
Proceedings of the National Academy of Sciences of the United States of America112(14)E1734-43(2015 Apr)

Embryonic stem cell differentiation requires full length Chd1.
Piatti P, Lim CY, Nat R, Villunger A, Geley S, Shue YT, Soratroi C, Moser M, Lusser A
Scientific reports58007(2015 Jan)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings indicate that CHD1 plays important roles in mouse early embryogenesis via activation of Hmgpi at zygotic gene activation.
Title: CHD1 acts via the Hmgpi pathway to regulate mouse early embryogenesis.

Chd1-driven elevation in transcriptional output is essential for the developmental transition from endothelium to definitive hematopoiesis in the mouse embryo.
Title: Emergence of hematopoietic stem and progenitor cells involves a Chd1-dependent increase in total nascent transcription.

Chd1 promotes a globally elevated transcriptional output required to sustain the distinctly rapid growth of the mouse epiblast.
Title: Chd1 is essential for the high transcriptional output and rapid growth of the mouse epiblast.

These results underscore the importance of Chd1 for the regulation of pluripotency in ESCs and provide evidence for a hitherto unrecognized critical role of the phosphorylated N-terminal SRR for full functionality of Chd1.
Title: Embryonic stem cell differentiation requires full length Chd1.

find that Chd1 evicts nucleosomes downstream of the promoter in order to overcome the nucleosomal barrier and enable PolII promoter escape, thus providing mechanistic insight into the role of Chd1 in transcription and pluripotency
Title: The nucleosomal barrier to promoter escape by RNA polymerase II is overcome by the chromatin remodeler Chd1.

The following Chd1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Chd1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OMu00615	NM_007690.3 Latest version!	Mus musculus chromodomain helicase DNA binding protein 1 (Chd1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1259.30 $1799.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Chd1 ( NM_007690.3 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_031716.2 Mus musculus chromodomain helicase DNA binding protein 1 (Chd1), mRNA.

Price & Availability↑

CloneID	OMu00615
Clone ID Related Accession (Same CDS sequence)	NM_007690.3
Accession Version	NM_007690.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5136bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-03
Organism	Mus musculus(house mouse)
Product	chromodomain-helicase-DNA-binding protein 1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CT033750.18 and AC102745.10. On Jun 17, 2009 this sequence version replaced NM_007690.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: L10410.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849381, SAMN00849383 [ECO:0000348] ##Evidence-Data-END##

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAATGGCA GCGGAGAATC AAGTCAGTCA 
GGTGATGATT GTGGGTCAGC ATCAGGCTCT GGATCTGGCT CGAGTTCTGG CAGCAGCAGT 
GACGGAAGCA GCAGCCAATC CGGGAGCAGC GACTCTGATT CTGGCTCTGA CTCAGGAAGT 
CAATCAGAGT CTGAATCAGA CACATCCCGA GAGAACAAGG TTCAAGCAAA ACCACCAAAA 
GTCGACGGAG CCGAGTTTTG GAAATCTAGC CCCAGTATTC TGGCTGTCCA GAGATCTGCA 
ATGCTTAGGA AGCAGCCACA GCAGGCCCAG CAGCAGCGCC CAGCTTCATC TAATAGTGGA 
TCCGAAGAAG ACTCGTCCAG CAGTGAAGAC TCCGACGACT CGTCCAGCGG TGCCAAGAGG 
AAGAAGCACA ATGATGAAGA CTGGCAGATG TCTGGGTCCG GATCTCCATC TCAGTCTGGT 
TCAGACTCAG AATCTGAAGA AGAGCGAGAT AAAAGCAGCT GCGACGGGAC AGAGTCCGAC 
TACGAGCCGA AAAACAAAGT CAGAAGCCGA AAGCCTCAGA ATAGATCTAA GTCAAAAAAT 
GGGAAAAAAA TTCTTGGACA AAAAAAGAGA CAGATTGATT CATCTGAGGA TGAAGATGAT 
GAAGATTATG ATAATGATAA ACGAAGCTCT CGCCGCCAAG CCACCGTCAA TGTGAGCTAC 
AAGGAGGATG AAGAAATGAA AACCGACTCC GATGACCTGC TGGAGGTCTG CGGCGAGGAC 
GTCCCTCAGC CTGAGGACGA GGAGTTTGAG ACAATAGAGA GGGTTATGGA TTGCAGAGTG 
GGGCGGAAAG GAGCTACTGG TGCTACTACA ACCATTTATG CTGTCGAAGC AGATGGTGAC 
CCAAATGCAG GATTTGAAAG AAACAAAGAG CCAGGAGACA TACAGTATTT AATTAAGTGG 
AAAGGATGGT CTCACATCCA CAACACATGG GAGACAGAAG AGACCCTGAA GCAGCAGAAC 
GTTAGAGGGA TGAAAAAATT GGATAATTAT AAGAAAAAAG ATCAAGAGAC GAAACGATGG 
CTGAAAAATG CTTCTCCAGA AGATGTGGAA TATTATAATT GCCAGCAAGA GCTTACAGAT 
GATCTACACA AACAGTATCA GATAGTGGAG CGCATAATTG CTCATTCCAA TCAAAAATCA 
GCAGCTGGTC TTCCTGATTA TTATTGCAAA TGGCAGGGGC TTCCATACTC AGAGTGCAGC 
TGGGAGGATG GAGCTCTCAT TTCCAAAAAG TTTCAGACAT GCATCGATGA ATATTTTAGC 
AGGAATCAGT CAAAAACGAC ACCTTTTAAA GATTGCAAAG TATTGAAACA AAGACCAAGA 
TTTGTAGCTC TGAAGAAACA ACCATCCTAT ATTGGAGGAC ATGAGGGCTT AGAACTGAGA 
GACTATCAGC TGAATGGTTT AAACTGGCTC GCTCACTCTT GGTGCAAAGG AAATAGTTGC 
ATACTTGCTG ATGAAATGGG CCTTGGGAAA ACAATACAGA CGATCTCATT TTTGAACTAT 
TTGTTCCATG AACATCAGTT ATATGGGCCG TTTCTACTAG TTGTCCCGCT CTCCACTCTG 
ACTTCCTGGC AGAGGGAGAT ACAGACGTGG GCGTCTCAGA TGAATGCTGT GGTTTACTTA 
GGCGACATTA ACAGCAGAAA CATGATAAGA ACTCATGAAT GGATGCATCC CCAGACCAAA 
CGGTTAAAAT TTAATATACT TTTAACAACG TATGAAATTT TATTGAAGGA TAAGGCATTC 
CTTGGTGGTC TGAATTGGGC ATTCATAGGT GTTGATGAAG CGCATCGATT AAAGAATGAT 
GATTCCCTTC TGTACAAAAC TTTAATCGAC TTTAAATCTA ACCATCGCCT TCTGATCACT 
GGAACCCCTC TACAGAACTC CCTGAAGGAG CTCTGGTCAC TGCTGCACTT CATTATGCCG 
GAGAAGTTTT CTTCATGGGA AGATTTTGAA GAAGAACATG GCAAAGGCAG AGAATATGGT 
TATGCAAGCC TCCACAAGGA GCTTGAGCCA TTTCTGTTAC GCAGAGTTAA GAAAGATGTG 
GAAAAATCTC TTCCTGCCAA GGTGGAGCAG ATTTTAAGAA TGGAGATGAG TGCTTTACAG 
AAGCAATATT ACAAGTGGAT TTTAACTAGG AATTATAAAG CCCTCAGCAA AGGTTCCAAG 
GGCAGTACCT CAGGCTTTTT GAACATTATG ATGGAGCTAA AGAAATGTTG TAACCATTGC 
TACCTCATTA AACCACCAGA TAATAATGAA TTCTATAATA AACAGGAGGC CTTACAACAC 
TTAATCCGTA GTAGCGGAAA ACTCATCCTT CTCGACAAGC TGTTGATTCG CCTAAGAGAA 
AGAGGCAACC GAGTGCTCAT TTTCTCTCAG ATGGTGCGGA TGTTAGACAT ACTCGCAGAG 
TATTTGAAGT ACCGCCAGTT CCCCTTTCAA AGATTAGATG GATCGATAAA AGGGGAGCTG 
AGGAAGCAGG CTTTGGATCA CTTTAATGCC GAGGGCTCAG AGGATTTCTG CTTTTTGCTT 
TCTACCCGAG CTGGGGGTTT AGGCATTAAC CTAGCCTCTG CTGACACCGT TGTTATATTC 
GATTCTGATT GGAATCCACA AAATGATCTT CAGGCTCAGG CAAGAGCTCA CCGAATTGGA 
CAGAAGAAAC AGGTGAATAT ATATCGCTTG GTTACAAAGG GATCAGTTGA AGAAGATATT 
CTTGAAAGGG CCAAAAAGAA AATGGTTTTG GATCATCTTG TTATTCAAAG AATGGATACC 
ACTGGGAAGA CAGTGCTGCA CACAGGCTCG GCTCCGTCAA GTTCCACCCC CTTCAATAAA 
GAGGAGTTAT CCGCCATTTT AAAATTCGGT GCTGAGGAGC TCTTTAAGGA GCCTGAAGGA 
GAGGAGCAGG AGCCACAGGA AATGGATATC GATGAAATCC TGAAGAGAGC CGAGACTCAC 
GAGAACGAGC CAGGCCCACT GAGTGTGGGG GATGAGCTGC TTTCTCAGTT TAAGGTTGCC 
AATTTTTCAA ATATGGATGA AGATGACATT GAATTGGAAC CTGAAAGAAA TTCAAAGAAC 
TGGGAGGAGA TCATTCCAGA AGAGCAAAGA CGGCGACTAG AAGAGGAGGA GAGACAAAAG 
GAACTGGAGG AAATTTATAT GCTCCCGAGA ATGAGAAACT GTGCAAAGCA GATAAGTTTC 
AATGGAAGTG AAGGGAGGCG GAGTAGAAGC AGGAGATATT CTGGATCTGA TAGTGATTCA 
ATCTCGGAAA GGAAACGGCC GAAGAAACGT GGGCGACCCC GCACTATCCC TCGGGAGAAT 
ATTAAAGGAT TTAGTGATGC GGAGATTCGG CGGTTTATCA AGAGCTATAA GAAATTTGGT 
GGCCCCCTGG AGAGGTTAGA TGCAATTGCT CGAGATGCTG AATTGGTTGA TAAGTCAGAA 
ACAGATCTCA GAAGACTAGG AGAACTGGTG CATAATGGTT GTGTTAAAGC TTTAAAAGAC 
AGTTCTTCAG GAACAGAGCG AGCAGGTGGC AGACTTGGAA AAGTGAAGGG GCCAACATTC 
CGCATCTCTG GAGTCCAAGT GAATGCCAAG CTGGTCATTG CCCATGAGGA TGAGCTGATC 
CCTCTGCATA AGTCCATCCC TTCGGACCCG GAGGAGAGGA AGCAGTATAC AATCCCCTGC 
CATACAAAGG CTGCGCACTT TGATATAGAC TGGGGCAAAG AAGATGATTC TAATTTGTTA 
ATTGGTATCT ATGAGTATGG CTATGGAAGC TGGGAAATGA TTAAAATGGA TCCAGACCTC 
AGTTTAACAC ACAAGATTCT TCCAGATGAT CCTGATAAAA AACCACAAGC AAAACAGTTA 
CAGACCCGTG CAGACTACCT CATCAAACTA CTTAGCAGAG ATCTTGCAAA AAGAGAGGCT 
CAGAGACTTT GTGGTGCGGG AGGTTCAAAG AGGAGAAAAA CGAGAGCTAA AAAGAGTAAG 
GCAATGAAGT CCATCAAAGT GAAAGAGGAA ATAAAGAGTG ACTCGTCGCC CCTGCCTTCA 
GAGAAGTCTG ACGAGGATGA CGATAAACTG AATGACTCCA AGCCTGAAAG TAAAGACCGA 
TCCAAAAAGT CTGTAGTGTC CGATGCTCCC GTTCACATCA CTGCGAGTGG AGAGCCCGTT 
CCCATAGCTG AAGAGTCTGA AGAGCTGGAT CAGAAGACAT TCAGTATTTG TAAAGAAAGA 
ATGAGACCGG TGAAAGCAGC TTTGAAACAA CTTGACAGGC CTGAGAAAGG CCTTTCAGAA 
AGAGAGCAGC TGGAACACAC TAGACAGTGC TTAATCAAGA TCGGAGACCA TATCACTGAA 
TGCTTGAAGG AATATTCCAA TCCTGAACAA ATTAAGCAGT GGAGGAAAAA CCTGTGGATT 
TTTGTATCTA AGTTTACTGA GTTTGATGCA AGGAAATTAC ATAAATTATA TAAGCATGCT 
ATTAAAAAAC GACAAGAATC TCAGCAAAAC AGTGACCAGA ATAGCAATGT TGCTACCACT 
CATGTGATTA GGAATCCAGA TATGGAAAGG TTAAAAGAGA ATACAAATCA TGATGACAGT 
AGCAGGGACA GCTATTCTTC TGACAGACAC TTATCTCAGT ACCATGATCA TCACAAGGAC 
CGCCATCAGG GAGATTCTTA TAAAAAGAGT GACTCTCGGA AGAGACCCTA CTCCTCATTT 
AGCAATGGCA AAGACCACCG CGAGTGGGAT CACTACAGGC AAGACAGCAG GTACTATAGT 
GACCGAGAGA AACACAGAAA ACTGGATGAC CACAGGAGTC GAGAGCACAG GCCAAGTTTG 
GAAGGAGGCT TAAAGGACAG GTGTCACTCT GACCACCGAT CTCACTCGGA CCATCGAATG 
CACTCAGACC ATCGCTCAAG CTCCGAGCAC ACACATCATA AATCCTCCAG GGATTATCGG 
TATCTCTCAG ATTGGCAGTT GGACCACCGA GCTGCCAGCA GTGGCCCTAG GTCACCTTTA 
GATCAGAGGT CTCCATATGG CTCCAGGTCC CCATTTGAAC ATTCAGCTGA ACACAGAAGT 
ACGCCTGAAC ACACCTGGAG TAGTCGGAAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_031716.2
CDS	494..5629
Translation	MNGHSDEESVRNGSGESSQSGDDCGSASGSGSGSSSGSSSDGSSSQSGSSDSDSGSDSGSQSESESDTSRENKVQAKPPKVDGAEFWKSSPSILAVQRSAMLRKQPQQAQQQRPASSNSGSEEDSSSSEDSDDSSSGAKRKKHNDEDWQMSGSGSPSQSGSDSESEEERDKSSCDGTESDYEPKNKVRSRKPQNRSKSKNGKKILGQKKRQIDSSEDEDDEDYDNDKRSSRRQATVNVSYKEDEEMKTDSDDLLEVCGEDVPQPEDEEFETIERVMDCRVGRKGATGATTTIYAVEADGDPNAGFERNKEPGDIQYLIKWKGWSHIHNTWETEETLKQQNVRGMKKLDNYKKKDQETKRWLKNASPEDVEYYNCQQELTDDLHKQYQIVERIIAHSNQKSAAGLPDYYCKWQGLPYSECSWEDGALISKKFQTCIDEYFSRNQSKTTPFKDCKVLKQRPRFVALKKQPSYIGGHEGLELRDYQLNGLNWLAHSWCKGNSCILADEMGLGKTIQTISFLNYLFHEHQLYGPFLLVVPLSTLTSWQREIQTWASQMNAVVYLGDINSRNMIRTHEWMHPQTKRLKFNILLTTYEILLKDKAFLGGLNWAFIGVDEAHRLKNDDSLLYKTLIDFKSNHRLLITGTPLQNSLKELWSLLHFIMPEKFSSWEDFEEEHGKGREYGYASLHKELEPFLLRRVKKDVEKSLPAKVEQILRMEMSALQKQYYKWILTRNYKALSKGSKGSTSGFLNIMMELKKCCNHCYLIKPPDNNEFYNKQEALQHLIRSSGKLILLDKLLIRLRERGNRVLIFSQMVRMLDILAEYLKYRQFPFQRLDGSIKGELRKQALDHFNAEGSEDFCFLLSTRAGGLGINLASADTVVIFDSDWNPQNDLQAQARAHRIGQKKQVNIYRLVTKGSVEEDILERAKKKMVLDHLVIQRMDTTGKTVLHTGSAPSSSTPFNKEELSAILKFGAEELFKEPEGEEQEPQEMDIDEILKRAETHENEPGPLSVGDELLSQFKVANFSNMDEDDIELEPERNSKNWEEIIPEEQRRRLEEEERQKELEEIYMLPRMRNCAKQISFNGSEGRRSRSRRYSGSDSDSISERKRPKKRGRPRTIPRENIKGFSDAEIRRFIKSYKKFGGPLERLDAIARDAELVDKSETDLRRLGELVHNGCVKALKDSSSGTERAGGRLGKVKGPTFRISGVQVNAKLVIAHEDELIPLHKSIPSDPEERKQYTIPCHTKAAHFDIDWGKEDDSNLLIGIYEYGYGSWEMIKMDPDLSLTHKILPDDPDKKPQAKQLQTRADYLIKLLSRDLAKREAQRLCGAGGSKRRKTRAKKSKAMKSIKVKEEIKSDSSPLPSEKSDEDDDKLNDSKPESKDRSKKSVVSDAPVHITASGEPVPIAEESEELDQKTFSICKERMRPVKAALKQLDRPEKGLSEREQLEHTRQCLIKIGDHITECLKEYSNPEQIKQWRKNLWIFVSKFTEFDARKLHKLYKHAIKKRQESQQNSDQNSNVATTHVIRNPDMERLKENTNHDDSSRDSYSSDRHLSQYHDHHKDRHQGDSYKKSDSRKRPYSSFSNGKDHREWDHYRQDSRYYSDREKHRKLDDHRSREHRPSLEGGLKDRCHSDHRSHSDHRMHSDHRSSSEHTHHKSSRDYRYLSDWQLDHRAASSGPRSPLDQRSPYGSRSPFEHSAEHRSTPEHTWSSRKT

Target ORF information:

RefSeq Version	NM_007690.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus chromodomain helicase DNA binding protein 1 (Chd1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_007690.3

ORF Insert Sequence:

ATGAATGGAC ACAGTGATGA AGAAAGTGTT AGAAATGGCA GCGGAGAATC AAGTCAGTCA 
GGTGATGATT GTGGGTCAGC ATCAGGCTCT GGATCTGGCT CGAGTTCTGG CAGCAGCAGT 
GACGGAAGCA GCAGCCAATC CGGGAGCAGC GACTCTGATT CTGGCTCTGA CTCAGGAAGT 
CAATCAGAGT CTGAATCAGA CACATCCCGA GAGAACAAGG TTCAAGCAAA ACCACCAAAA 
GTCGACGGAG CCGAGTTTTG GAAATCTAGC CCCAGTATTC TGGCTGTCCA GAGATCTGCA 
ATGCTTAGGA AGCAGCCACA GCAGGCCCAG CAGCAGCGCC CAGCTTCATC TAATAGTGGA 
TCCGAAGAAG ACTCGTCCAG CAGTGAAGAC TCCGACGACT CGTCCAGCGG TGCCAAGAGG 
AAGAAGCACA ATGATGAAGA CTGGCAGATG TCTGGGTCCG GATCTCCATC TCAGTCTGGT 
TCAGACTCAG AATCTGAAGA AGAGCGAGAT AAAAGCAGCT GCGACGGGAC AGAGTCCGAC 
TACGAGCCGA AAAACAAAGT CAGAAGCCGA AAGCCTCAGA ATAGATCTAA GTCAAAAAAT 
GGGAAAAAAA TTCTTGGACA AAAAAAGAGA CAGATTGATT CATCTGAGGA TGAAGATGAT 
GAAGATTATG ATAATGATAA ACGAAGCTCT CGCCGCCAAG CCACCGTCAA TGTGAGCTAC 
AAGGAGGATG AAGAAATGAA AACCGACTCC GATGACCTGC TGGAGGTCTG CGGCGAGGAC 
GTCCCTCAGC CTGAGGACGA GGAGTTTGAG ACAATAGAGA GGGTTATGGA TTGCAGAGTG 
GGGCGGAAAG GAGCTACTGG TGCTACTACA ACCATTTATG CTGTCGAAGC AGATGGTGAC 
CCAAATGCAG GATTTGAAAG AAACAAAGAG CCAGGAGACA TACAGTATTT AATTAAGTGG 
AAAGGATGGT CTCACATCCA CAACACATGG GAGACAGAAG AGACCCTGAA GCAGCAGAAC 
GTTAGAGGGA TGAAAAAATT GGATAATTAT AAGAAAAAAG ATCAAGAGAC GAAACGATGG 
CTGAAAAATG CTTCTCCAGA AGATGTGGAA TATTATAATT GCCAGCAAGA GCTTACAGAT 
GATCTACACA AACAGTATCA GATAGTGGAG CGCATAATTG CTCATTCCAA TCAAAAATCA 
GCAGCTGGTC TTCCTGATTA TTATTGCAAA TGGCAGGGGC TTCCATACTC AGAGTGCAGC 
TGGGAGGATG GAGCTCTCAT TTCCAAAAAG TTTCAGACAT GCATCGATGA ATATTTTAGC 
AGGAATCAGT CAAAAACGAC ACCTTTTAAA GATTGCAAAG TATTGAAACA AAGACCAAGA 
TTTGTAGCTC TGAAGAAACA ACCATCCTAT ATTGGAGGAC ATGAGGGCTT AGAACTGAGA 
GACTATCAGC TGAATGGTTT AAACTGGCTC GCTCACTCTT GGTGCAAAGG AAATAGTTGC 
ATACTTGCTG ATGAAATGGG CCTTGGGAAA ACAATACAGA CGATCTCATT TTTGAACTAT 
TTGTTCCATG AACATCAGTT ATATGGGCCG TTTCTACTAG TTGTCCCGCT CTCCACTCTG 
ACTTCCTGGC AGAGGGAGAT ACAGACGTGG GCGTCTCAGA TGAATGCTGT GGTTTACTTA 
GGCGACATTA ACAGCAGAAA CATGATAAGA ACTCATGAAT GGATGCATCC CCAGACCAAA 
CGGTTAAAAT TTAATATACT TTTAACAACG TATGAAATTT TATTGAAGGA TAAGGCATTC 
CTTGGTGGTC TGAATTGGGC ATTCATAGGT GTTGATGAAG CGCATCGATT AAAGAATGAT 
GATTCCCTTC TGTACAAAAC TTTAATCGAC TTTAAATCTA ACCATCGCCT TCTGATCACT 
GGAACCCCTC TACAGAACTC CCTGAAGGAG CTCTGGTCAC TGCTGCACTT CATTATGCCG 
GAGAAGTTTT CTTCATGGGA AGATTTTGAA GAAGAACATG GCAAAGGCAG AGAATATGGT 
TATGCAAGCC TCCACAAGGA GCTTGAGCCA TTTCTGTTAC GCAGAGTTAA GAAAGATGTG 
GAAAAATCTC TTCCTGCCAA GGTGGAGCAG ATTTTAAGAA TGGAGATGAG TGCTTTACAG 
AAGCAATATT ACAAGTGGAT TTTAACTAGG AATTATAAAG CCCTCAGCAA AGGTTCCAAG 
GGCAGTACCT CAGGCTTTTT GAACATTATG ATGGAGCTAA AGAAATGTTG TAACCATTGC 
TACCTCATTA AACCACCAGA TAATAATGAA TTCTATAATA AACAGGAGGC CTTACAACAC 
TTAATCCGTA GTAGCGGAAA ACTCATCCTT CTCGACAAGC TGTTGATTCG CCTAAGAGAA 
AGAGGCAACC GAGTGCTCAT TTTCTCTCAG ATGGTGCGGA TGTTAGACAT ACTCGCAGAG 
TATTTGAAGT ACCGCCAGTT CCCCTTTCAA AGATTAGATG GATCGATAAA AGGGGAGCTG 
AGGAAGCAGG CTTTGGATCA CTTTAATGCC GAGGGCTCAG AGGATTTCTG CTTTTTGCTT 
TCTACCCGAG CTGGGGGTTT AGGCATTAAC CTAGCCTCTG CTGACACCGT TGTTATATTC 
GATTCTGATT GGAATCCACA AAATGATCTT CAGGCTCAGG CAAGAGCTCA CCGAATTGGA 
CAGAAGAAAC AGGTGAATAT ATATCGCTTG GTTACAAAGG GATCAGTTGA AGAAGATATT 
CTTGAAAGGG CCAAAAAGAA AATGGTTTTG GATCATCTTG TTATTCAAAG AATGGATACC 
ACTGGGAAGA CAGTGCTGCA CACAGGCTCG GCTCCGTCAA GTTCCACCCC CTTCAATAAA 
GAGGAGTTAT CCGCCATTTT AAAATTCGGT GCTGAGGAGC TCTTTAAGGA GCCTGAAGGA 
GAGGAGCAGG AGCCACAGGA AATGGATATC GATGAAATCC TGAAGAGAGC CGAGACTCAC 
GAGAACGAGC CAGGCCCACT GAGTGTGGGG GATGAGCTGC TTTCTCAGTT TAAGGTTGCC 
AATTTTTCAA ATATGGATGA AGATGACATT GAATTGGAAC CTGAAAGAAA TTCAAAGAAC 
TGGGAGGAGA TCATTCCAGA AGAGCAAAGA CGGCGACTAG AAGAGGAGGA GAGACAAAAG 
GAACTGGAGG AAATTTATAT GCTCCCGAGA ATGAGAAACT GTGCAAAGCA GATAAGTTTC 
AATGGAAGTG AAGGGAGGCG GAGTAGAAGC AGGAGATATT CTGGATCTGA TAGTGATTCA 
ATCTCGGAAA GGAAACGGCC GAAGAAACGT GGGCGACCCC GCACTATCCC TCGGGAGAAT 
ATTAAAGGAT TTAGTGATGC GGAGATTCGG CGGTTTATCA AGAGCTATAA GAAATTTGGT 
GGCCCCCTGG AGAGGTTAGA TGCAATTGCT CGAGATGCTG AATTGGTTGA TAAGTCAGAA 
ACAGATCTCA GAAGACTAGG AGAACTGGTG CATAATGGTT GTGTTAAAGC TTTAAAAGAC 
AGTTCTTCAG GAACAGAGCG AGCAGGTGGC AGACTTGGAA AAGTGAAGGG GCCAACATTC 
CGCATCTCTG GAGTCCAAGT GAATGCCAAG CTGGTCATTG CCCATGAGGA TGAGCTGATC 
CCTCTGCATA AGTCCATCCC TTCGGACCCG GAGGAGAGGA AGCAGTATAC AATCCCCTGC 
CATACAAAGG CTGCGCACTT TGATATAGAC TGGGGCAAAG AAGATGATTC TAATTTGTTA 
ATTGGTATCT ATGAGTATGG CTATGGAAGC TGGGAAATGA TTAAAATGGA TCCAGACCTC 
AGTTTAACAC ACAAGATTCT TCCAGATGAT CCTGATAAAA AACCACAAGC AAAACAGTTA 
CAGACCCGTG CAGACTACCT CATCAAACTA CTTAGCAGAG ATCTTGCAAA AAGAGAGGCT 
CAGAGACTTT GTGGTGCGGG AGGTTCAAAG AGGAGAAAAA CGAGAGCTAA AAAGAGTAAG 
GCAATGAAGT CCATCAAAGT GAAAGAGGAA ATAAAGAGTG ACTCGTCGCC CCTGCCTTCA 
GAGAAGTCTG ACGAGGATGA CGATAAACTG AATGACTCCA AGCCTGAAAG TAAAGACCGA 
TCCAAAAAGT CTGTAGTGTC CGATGCTCCC GTTCACATCA CTGCGAGTGG AGAGCCCGTT 
CCCATAGCTG AAGAGTCTGA AGAGCTGGAT CAGAAGACAT TCAGTATTTG TAAAGAAAGA 
ATGAGACCGG TGAAAGCAGC TTTGAAACAA CTTGACAGGC CTGAGAAAGG CCTTTCAGAA 
AGAGAGCAGC TGGAACACAC TAGACAGTGC TTAATCAAGA TCGGAGACCA TATCACTGAA 
TGCTTGAAGG AATATTCCAA TCCTGAACAA ATTAAGCAGT GGAGGAAAAA CCTGTGGATT 
TTTGTATCTA AGTTTACTGA GTTTGATGCA AGGAAATTAC ATAAATTATA TAAGCATGCT 
ATTAAAAAAC GACAAGAATC TCAGCAAAAC AGTGACCAGA ATAGCAATGT TGCTACCACT 
CATGTGATTA GGAATCCAGA TATGGAAAGG TTAAAAGAGA ATACAAATCA TGATGACAGT 
AGCAGGGACA GCTATTCTTC TGACAGACAC TTATCTCAGT ACCATGATCA TCACAAGGAC 
CGCCATCAGG GAGATTCTTA TAAAAAGAGT GACTCTCGGA AGAGACCCTA CTCCTCATTT 
AGCAATGGCA AAGACCACCG CGAGTGGGAT CACTACAGGC AAGACAGCAG GTACTATAGT 
GACCGAGAGA AACACAGAAA ACTGGATGAC CACAGGAGTC GAGAGCACAG GCCAAGTTTG 
GAAGGAGGCT TAAAGGACAG GTGTCACTCT GACCACCGAT CTCACTCGGA CCATCGAATG 
CACTCAGACC ATCGCTCAAG CTCCGAGCAC ACACATCATA AATCCTCCAG GGATTATCGG 
TATCTCTCAG ATTGGCAGTT GGACCACCGA GCTGCCAGCA GTGGCCCTAG GTCACCTTTA 
GATCAGAGGT CTCCATATGG CTCCAGGTCC CCATTTGAAC ATTCAGCTGA ACACAGAAGT 
ACGCCTGAAC ACACCTGGAG TAGTCGGAAG ACATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

CHD1 loss sensitizes prostate cancer to DNA damaging therapy by promoting error-prone double-strand break repair.
Annals of oncology : official journal of the European Society for Medical Oncology28(7)1495-1507(2017 Jul)
Shenoy TR,Boysen G,Wang MY,Xu QZ,Guo W,Koh FM,Wang C,Zhang LZ,Wang Y,Gil V,Aziz S,Christova R,Rodrigues DN,Crespo M,Rescigno P,Tunariu N,Riisnaes R,Zafeiriou Z,Flohr P,Yuan W,Knight E,Swain A,Ramalho-Santos M,Xu DY,de Bono J,Wu H

High-throughput discovery of novel developmental phenotypes.
Nature537(7621)508-514(2016 09)
Dickinson ME,Flenniken AM,Ji X,Teboul L,Wong MD,White JK,Meehan TF,Weninger WJ,Westerberg H,Adissu H,Baker CN,Bower L,Brown JM,Caddle LB,Chiani F,Clary D,Cleak J,Daly MJ,Denegre JM,Doe B,Dolan ME,Edie SM,Fuchs H,Gailus-Durner V,Galli A,Gambadoro A,Gallegos J,Guo S,Horner NR,Hsu CW,Johnson SJ,Kalaga S,Keith LC,Lanoue L,Lawson TN,Lek M,Mark M,Marschall S,Mason J,McElwee ML,Newbigging S,Nutter LM,Peterson KA,Ramirez-Solis R,Rowland DJ,Ryder E,Samocha KE,Seavitt JR,Selloum M,Szoke-Kovacs Z,Tamura M,Trainor AG,Tudose I,Wakana S,Warren J,Wendling O,West DB,Wong L,Yoshiki A,,,,,,,,,MacArthur DG,Tocchini-Valentini GP,Gao X,Flicek P,Bradley A,Skarnes WC,Justice MJ,Parkinson HE,Moore M,Wells S,Braun RE,Svenson KL,de Angelis MH,Herault Y,Mohun T,Mallon AM,Henkelman RM,Brown SD,Adams DJ,Lloyd KC,McKerlie C,Beaudet AL,Bu?an M,Murray SA

CHD1 acts via the Hmgpi pathway to regulate mouse early embryogenesis.
Development (Cambridge, England)142(13)2375-84(2015 Jul)
Suzuki S,Nozawa Y,Tsukamoto S,Kaneko T,Manabe I,Imai H,Minami N

Emergence of hematopoietic stem and progenitor cells involves a Chd1-dependent increase in total nascent transcription.
Proceedings of the National Academy of Sciences of the United States of America112(14)E1734-43(2015 Apr)
Koh FM,Lizama CO,Wong P,Hawkins JS,Zovein AC,Ramalho-Santos M

Embryonic stem cell differentiation requires full length Chd1.
Scientific reports58007(2015 Jan)
Piatti P,Lim CY,Nat R,Villunger A,Geley S,Shue YT,Soratroi C,Moser M,Lusser A

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Chd1 cDNA ORF clone, Mus musculus(house mouse)

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Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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