ORF » Species Summary » Schizosaccharomyces pombe » lrs1 cDNA ORF clone

lrs1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following lrs1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the lrs1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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OSc103962 NM_001020311.2
Latest version!		 Schizosaccharomyces pombe putative leucine--tRNA (Leu) ligase Lrs1 (lrs1), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    lrs1 ( NM_001020311.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_594882.1 Schizosaccharomyces pombe putative leucine--tRNA (Leu) ligase Lrs1 (lrs1), mRNA.
    Price & Availability↑
    CloneID OSc103962
    Clone ID Related Accession (Same CDS sequence) NM_001020311.2
    Accession Version NM_001020311.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3336bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product putative leucine--tRNA (Leu) ligase Lrs1
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020311.1. 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTACGA CTGAACCTTC CGTCGAGCAA CTAGAGACGA AGACTGCAAA GTTGAAGTTG 
    GAAAACACGA CGAAGCGTGA CACTCTAATT GAGTTGGAGA AAAAATATCA ACAAAAATGG 
    CAAGAAGAAA AGGCATTTGA AGTCGATGCT CCTTTGGAAG ATGTTCCGAT CGATGAACTT 
    CGTAAGAAAT ATCCTAAATT TTTTGGTAAC ATGCCTTATC CTTATATGAA TGGTGCATTG 
    CATCTTGGCC ATGCCTTCAC TTTGTCTAAG GTCGAGTTTA CTACAGCTTT TGAACGCTTG 
    AATGGAAAGC GAGTGTTGTT TCCCATGGGC TTTCACTGCA CCGGTATGCC TATTTGCGCT 
    AGTGCTGACC GTTTGTCACG TGAAATAGAG ATGTTTGGAC CCAGCTTTGA CGTTCCTGAA 
    GAAAAGGAAG AAGAGGTAGA GGTCGAAGTT AAGACTCCTA ATGCTCGCGA AGATGTCACT 
    AAGCACAGCG GTAAAAAGAG TAAGGCTGCG GCTAAAACAG CTGCTGTAAA GTACCAATTT 
    CAAATTATGG AATCTCTTGG TGTTCCCCGG ACTGAAATTC ACAAGTTTGC TGATGCAAAG 
    TACTGGCTCT CATATTTCCC TCCCTTGTGC CAAAGAGATT GTACTGAATT TGGTCTCGGT 
    ATTGACTGGC GTCGTTCCTT TATCACTACT GATGTCAATC CTTACTATGA CTCCTTTGTA 
    CGTTGGCAAG TAAATCATTT GCACGATTCT GGGAAAATTA AGTTTGGTGA GCGCTATACT 
    GTTTATTCCA TCAAGGACGG TCAACCCTGT ATGGATCACG ACCGTAAAAG TGGTGAAGGT 
    GTTGGTCCTC AGGAGTACAC TGGTATCAAA ATGGAAGTTT TGGAGTTCCC CGAGGCTGCT 
    CGTAAAGCTC TCCAATCCAT TGATCTTTCC AATAAGAAGG TCTGTATGAT TGCAGCAACG 
    CTCCGTCCAG AAACCATGTA TGGCCAAACA AATTGCTATG TTGGCCCCAA CATCACCTAT 
    GGCATTTACG AAAGTAATGT TCCCAATGAG CTTTTCATTT GCACTCGTCG TGCTGCTAAC 
    AACATGGCAT ACCAAAAGCT TTCAAAGGAA CGTGGTGTAG TCAGCGAACT TGGAACCATT 
    AAGGGACAAG ACCTTATTGG CGCCCTTGTT AATGCACCTC TGTCCGTGCA CAAACAAGTT 
    TATGTTTTAC CTATGGAAAC AGTTTTGGCT ACTAAGGGTA CTGGCGTGGT CACCTCTGTG 
    CCTAGCGACT CTCCTGATGA CTTTGCTACT TTAACTGAGC TTCGCAAAAA GGCTGAATTT 
    TATCACTTGA ATCCCGAGTG GATGAAGTAC GAAGCTGTCC CTATTATTCG CACTCCTTCT 
    TATGGTGACA TGTGTGCTGA GTTTTTGTGC AAAAAACTTA AGATTCAAAG TCCTAAGGAT 
    GTTAAACAAT TGGCTCAAGC TAAGGAGCTT GCTTATAAAG AGTGTTTCTA CCAAGGTACG 
    ATGATTATCG GTAAATATTC CGGTGAGAAG GTCGAGACAG CCAAGCCCAA GGTTCGTAAG 
    GAGTTGATTG ATCAAGGCCT TGCTTTTGTT TATAATGAAC CCGAAGGACA AGTTATTAGT 
    CGCAGTGGTG ATGATTGTAT TGTTGCCTTG TGCGACCAAT GGTTTTTGGA TTATGGCGAG 
    GCTTCTTGGA AGGCCGTAAC CGAGAAGGCA TTGGATCGTT TGAACACTTT CTCACCCGAG 
    GTCCGTAATG GTTTTTTGAA AACCTTGGAT TGGCTTAGCC AATGGGCTTG TGCTCGTTCT 
    TATGGTTTAG GTACTCGCTT GCCATGGGAT CCTCAGTTCC TTGTTGAAAG TTTGACCGAC 
    TCGACCATCT ACATGGCCTA TTATACAATT TGCCATCTTT TGCATAGCGA TGTATATGGT 
    AAGGTACCGG GTGCTTTGAA CATTAAGCCT GAGCAAATGA CTCCTGAAGT ATGGGATCAT 
    GTTTTCCGCC AAGCACCAAA GCCTAAAAAC ACCAGCATAT CCGATGAAGC ATTGGCTCGT 
    CTTTGCCGTG AGTTCCAATA CTTTTATCCT TTTGATATTC GTGCTTCCGG TAAAGATTTG 
    GTTCCTAATC ATCTTACCTT CTGTTTGTAC ACCCATACCG CCATTTTTGA TGAGAAGTAT 
    TGGCCCAAAG GTATACGCGC CAATGGCCAT CTTCTCATGA ATGGTGAAAA GATGTCTAAA 
    TCCACTGGTA ACTTTATGAC TCTTCATGAG GCCACCAAGA AATTCGGTGC AGACGCAACT 
    CGTTTAGCTC TTGCTGATGC TGGTGATACT GTTGATGATG CAAACTTTGA AGAGGCTTTG 
    GCCAATTCTG CTATTCTTCG TCTGTATACC CAGGAAGCCT GGTGTAAGGA GATGATGGAA 
    AACCTTGACA ATTTACGCAC AGGCCCTTAC AACTTCCATG ATAAAGTTTT TGAAAACGAG 
    ATCAATCAAC TCATCGAATC ATCTAGAGAA GCTTTTTCTG CTACTCTTTT CAAGGCTGCC 
    TTGAAGAGTT GCTTTTACGA TCTTCAAAAT GCTCGTGATT GGTATCGTGA GGTAACCGCT 
    GATCGTAAAA TGCATCGCGA TCTTGTTTGT CGTTGGATAG AAACTCAAGT GCTTCTGCTG 
    GCTACATTTG CACCTCACTG GTCAGAACAC ATCTGGCTTA CAACTCTCAA GAAACCTCAG 
    AGTATCCATG TTTCTGGTCG TTTCCCCCAA GTTTCCAGCC CTGTAAACAC GGCTTTATCT 
    AACTCCTTGT TATACATCCG TACACTGAGC CGTGTAATTC GTGAAGCCGA GGCAGCTCAA 
    CTCAAGCGTC AAAAGAAAGG TAAAGGTATG CTTTTCGATC CCTCCAAGCC TAAGCGTCTT 
    ACTGTGTTCG TCGCTGAAAA GTTCCCCGAG TGGCAAGCTC AGTACGTTGC TCTTCTTCAA 
    AAATACTATA ATGAGTCTGA GAATAAGTTT GATGATAAAG CAATCATTTC TAGTGTTGAT 
    AAAAAGGAAA TGAAGCGCGC AATGCCCTTC ATTCAGCAAT TCAAACAATC TGTCATTAAC 
    CGTGGAGAGC ATGTGTCAGC CAATTCCATA TTTAGTCGTG AACTCGGATT CAATGAGCTT 
    GAAGTTTTGC GTGAGGTTAA GCCTTACCTT GTTCGAAACG TTGGTATTCA AGAGCTTCGC 
    ATTGTCCTTC TTCAAAAACC TGCAGATAAA TCTTCAGCTG CTATTGGTTT GGTTGAAAGT 
    GGATCGGATG CCGGTGCTAC TGTAGAGATT GCACCTAATT TTGCAAATAC CGTACCCGGT 
    CAGCCTACAT TTTTGTTTGA AAATGTCTCT GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594882.1
    CDS165..3500
    Translation

    Target ORF information:

    RefSeq Version NM_001020311.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe putative leucine--tRNA (Leu) ligase Lrs1 (lrs1), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001020311.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    ATGGCTACGA CTGAACCTTC CGTCGAGCAA CTAGAGACGA AGACTGCAAA GTTGAAGTTG 
    GAAAACACGA CGAAGCGTGA CACTCTAATT GAGTTGGAGA AAAAATATCA ACAAAAATGG 
    CAAGAAGAAA AGGCATTTGA AGTCGATGCT CCTTTGGAAG ATGTTCCGAT CGATGAACTT 
    CGTAAGAAAT ATCCTAAATT TTTTGGTAAC ATGCCTTATC CTTATATGAA TGGTGCATTG 
    CATCTTGGCC ATGCCTTCAC TTTGTCTAAG GTCGAGTTTA CTACAGCTTT TGAACGCTTG 
    AATGGAAAGC GAGTGTTGTT TCCCATGGGC TTTCACTGCA CCGGTATGCC TATTTGCGCT 
    AGTGCTGACC GTTTGTCACG TGAAATAGAG ATGTTTGGAC CCAGCTTTGA CGTTCCTGAA 
    GAAAAGGAAG AAGAGGTAGA GGTCGAAGTT AAGACTCCTA ATGCTCGCGA AGATGTCACT 
    AAGCACAGCG GTAAAAAGAG TAAGGCTGCG GCTAAAACAG CTGCTGTAAA GTACCAATTT 
    CAAATTATGG AATCTCTTGG TGTTCCCCGG ACTGAAATTC ACAAGTTTGC TGATGCAAAG 
    TACTGGCTCT CATATTTCCC TCCCTTGTGC CAAAGAGATT GTACTGAATT TGGTCTCGGT 
    ATTGACTGGC GTCGTTCCTT TATCACTACT GATGTCAATC CTTACTATGA CTCCTTTGTA 
    CGTTGGCAAG TAAATCATTT GCACGATTCT GGGAAAATTA AGTTTGGTGA GCGCTATACT 
    GTTTATTCCA TCAAGGACGG TCAACCCTGT ATGGATCACG ACCGTAAAAG TGGTGAAGGT 
    GTTGGTCCTC AGGAGTACAC TGGTATCAAA ATGGAAGTTT TGGAGTTCCC CGAGGCTGCT 
    CGTAAAGCTC TCCAATCCAT TGATCTTTCC AATAAGAAGG TCTGTATGAT TGCAGCAACG 
    CTCCGTCCAG AAACCATGTA TGGCCAAACA AATTGCTATG TTGGCCCCAA CATCACCTAT 
    GGCATTTACG AAAGTAATGT TCCCAATGAG CTTTTCATTT GCACTCGTCG TGCTGCTAAC 
    AACATGGCAT ACCAAAAGCT TTCAAAGGAA CGTGGTGTAG TCAGCGAACT TGGAACCATT 
    AAGGGACAAG ACCTTATTGG CGCCCTTGTT AATGCACCTC TGTCCGTGCA CAAACAAGTT 
    TATGTTTTAC CTATGGAAAC AGTTTTGGCT ACTAAGGGTA CTGGCGTGGT CACCTCTGTG 
    CCTAGCGACT CTCCTGATGA CTTTGCTACT TTAACTGAGC TTCGCAAAAA GGCTGAATTT 
    TATCACTTGA ATCCCGAGTG GATGAAGTAC GAAGCTGTCC CTATTATTCG CACTCCTTCT 
    TATGGTGACA TGTGTGCTGA GTTTTTGTGC AAAAAACTTA AGATTCAAAG TCCTAAGGAT 
    GTTAAACAAT TGGCTCAAGC TAAGGAGCTT GCTTATAAAG AGTGTTTCTA CCAAGGTACG 
    ATGATTATCG GTAAATATTC CGGTGAGAAG GTCGAGACAG CCAAGCCCAA GGTTCGTAAG 
    GAGTTGATTG ATCAAGGCCT TGCTTTTGTT TATAATGAAC CCGAAGGACA AGTTATTAGT 
    CGCAGTGGTG ATGATTGTAT TGTTGCCTTG TGCGACCAAT GGTTTTTGGA TTATGGCGAG 
    GCTTCTTGGA AGGCCGTAAC CGAGAAGGCA TTGGATCGTT TGAACACTTT CTCACCCGAG 
    GTCCGTAATG GTTTTTTGAA AACCTTGGAT TGGCTTAGCC AATGGGCTTG TGCTCGTTCT 
    TATGGTTTAG GTACTCGCTT GCCATGGGAT CCTCAGTTCC TTGTTGAAAG TTTGACCGAC 
    TCGACCATCT ACATGGCCTA TTATACAATT TGCCATCTTT TGCATAGCGA TGTATATGGT 
    AAGGTACCGG GTGCTTTGAA CATTAAGCCT GAGCAAATGA CTCCTGAAGT ATGGGATCAT 
    GTTTTCCGCC AAGCACCAAA GCCTAAAAAC ACCAGCATAT CCGATGAAGC ATTGGCTCGT 
    CTTTGCCGTG AGTTCCAATA CTTTTATCCT TTTGATATTC GTGCTTCCGG TAAAGATTTG 
    GTTCCTAATC ATCTTACCTT CTGTTTGTAC ACCCATACCG CCATTTTTGA TGAGAAGTAT 
    TGGCCCAAAG GTATACGCGC CAATGGCCAT CTTCTCATGA ATGGTGAAAA GATGTCTAAA 
    TCCACTGGTA ACTTTATGAC TCTTCATGAG GCCACCAAGA AATTCGGTGC AGACGCAACT 
    CGTTTAGCTC TTGCTGATGC TGGTGATACT GTTGATGATG CAAACTTTGA AGAGGCTTTG 
    GCCAATTCTG CTATTCTTCG TCTGTATACC CAGGAAGCCT GGTGTAAGGA GATGATGGAA 
    AACCTTGACA ATTTACGCAC AGGCCCTTAC AACTTCCATG ATAAAGTTTT TGAAAACGAG 
    ATCAATCAAC TCATCGAATC ATCTAGAGAA GCTTTTTCTG CTACTCTTTT CAAGGCTGCC 
    TTGAAGAGTT GCTTTTACGA TCTTCAAAAT GCTCGTGATT GGTATCGTGA GGTAACCGCT 
    GATCGTAAAA TGCATCGCGA TCTTGTTTGT CGTTGGATAG AAACTCAAGT GCTTCTGCTG 
    GCTACATTTG CACCTCACTG GTCAGAACAC ATCTGGCTTA CAACTCTCAA GAAACCTCAG 
    AGTATCCATG TTTCTGGTCG TTTCCCCCAA GTTTCCAGCC CTGTAAACAC GGCTTTATCT 
    AACTCCTTGT TATACATCCG TACACTGAGC CGTGTAATTC GTGAAGCCGA GGCAGCTCAA 
    CTCAAGCGTC AAAAGAAAGG TAAAGGTATG CTTTTCGATC CCTCCAAGCC TAAGCGTCTT 
    ACTGTGTTCG TCGCTGAAAA GTTCCCCGAG TGGCAAGCTC AGTACGTTGC TCTTCTTCAA 
    AAATACTATA ATGAGTCTGA GAATAAGTTT GATGATAAAG CAATCATTTC TAGTGTTGAT 
    AAAAAGGAAA TGAAGCGCGC AATGCCCTTC ATTCAGCAAT TCAAACAATC TGTCATTAAC 
    CGTGGAGAGC ATGTGTCAGC CAATTCCATA TTTAGTCGTG AACTCGGATT CAATGAGCTT 
    GAAGTTTTGC GTGAGGTTAA GCCTTACCTT GTTCGAAACG TTGGTATTCA AGAGCTTCGC 
    ATTGTCCTTC TTCAAAAACC TGCAGATAAA TCTTCAGCTG CTATTGGTTT GGTTGAAAGT 
    GGATCGGATG CCGGTGCTAC TGTAGAGATT GCACCTAATT TTGCAAATAC CGTACCCGGT 
    CAGCCTACAT TTTTGTTTGA AAATGTCTCT GCTTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L


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