SPAC11E3.05 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

The following SPAC11E3.05 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SPAC11E3.05 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
OSc102331 NM_001020361.2
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Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC11E3.05), mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID OSc102331
    Clone ID Related Accession (Same CDS sequence) NM_001020361.2
    Accession Version NM_001020361.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3972bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-04-02
    Organism Schizosaccharomyces pombe(fission yeast)
    Product ubiquitin-protein ligase E3
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001020361.1.

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGGAAT TACAGGGAGA CGATTCTTCA AGAAAGTCTC CTCCGAGTGA TTCGGTTGTA 
    AAAAATTCTT CGCCTATTGA TTATGAACAT TCATTGAAAT CTCTTCAAGA TGAACGTACC
    TTGAATTACC CCAATAAGCA ATTTAATTCT GAAAACCCAT CTTCATACTA TAATATGGAT
    GATAGCGGGG AATTATGCAA CTTTGAATTG GAGAAAGACA GTCTTGAATC AATGATCCAT
    GAATCGTCCA CTGCTCTAAG CGCTCAATCA AACACTGCAC AAGACGGTGA TCAACTAGCT
    TCATCGTCAA CAATCTCTAA AGATCACTCA GAAACGTTGG ACAATAAGCT AAACGATTCT
    AAGATATTAA TTAAAAAAGC TTCTAACTCC TTTGCAAATT TTTCAGAAAG TGTAAGGTCT
    AGTACCATTG TTGCATATTC TCAAACACCG ACACAAAGGT TGCAAGGTTT AACTTCCATA
    TCATCTTCTT CTTTTGACGA TGGTAGTTAT GGATCTCGTC GAATATCTTT TAACTCTCAG
    GGTGTTTCTG GTCGGTTGCG AAGAAATATG GATTCCACGG TCATCATCCC TTCCGAAGAT
    GAAGACTTGC AGTTACCATC GAATTCTAAC AGCAATGTGG AATACGGCCC TTTTGATAGT
    ACTACGTTTG ATAGACAATT ATCAATTGAA GTTAATCAAC CAGTGGGTGC TATGTCTTTA
    TCTCCTTGTG GTAGAGATAT AGCTTTAGCT TCACGTTATG GTTTACTAGT CTTAGATCTT
    GATAATCCTT ATAATCCACC AAGAATGCTA AGACATTCTA CTCCTTGGGA AGTTGCTGAT
    ACTCAGTGGA ATGTTCATGC CGCAAGAGAC CAATGGGTGG TTTCTACATC AAGTCAAAAA
    ACGATAGTAT GGAACCTTGC ACTACCAAAT GATCGTGCTA TAGAATTTAT GTTACATGGA
    CACACCCGCG CCGTCACAGA TATGAACTGG CATCGGCAAA ATCCTGATGT TCTTGCCACT
    TGTTCAATTG ATTCCTCTGT TCACTGTTGG GATTTGCGTA GTCCTCGATT TCCTGTTAAC
    TCCTTTTACG ATTGGCATAA CGGAGCTACT CAGGTAAAAT GGAATTATAA AAATCCCCAC
    ATACTTGCTT CTAGCCATGG ACGTTTAGTA AGGATTTGGG ACGATCGTTA TGGATCCGCC
    CCACTTCATA CAATTAAAAC TTCCGAAAAT ATTACTAAAA TTAATGGTCT TGAATTCAAT
    AGAGCATGTG AAACTCGCCT ATTAACTTGT GCTATGGATC GAACAGTTAA GTTTTGGAAC
    TATGAAAAGT CAACAGAGGA GCCTGAGCAT TTAATTACCA CTGATTCTCC AGTATGGAAA
    GCAAGGTTTA CTCCATTCGG GGACGGAGTT ATTTTGATGC CTCAGAGAGG AGACAATTCC
    GTACACATGT ACGATTGTCG TAATTTAGAC AAAGAAGGCC CCCGTGCTGT ACATAGATTT
    GCCGGTCATA CCGATCAAGT TAAAGAATTT CTTTGGAGGT GTCGAGGCGA AGATGTATTT
    GATAGAGATT TAAGAGATTT TCAATTGATC ACTTGGTCCA AGGATCACCA TGTTCGTTTA
    TGGCCTATTG GTAACGATAT ACTCAATTCA ATGGGACACG ATCGAACAAA ACCTGTTCCT
    TTTAAATTAA CACGGTTAGG CGCGAAATAT CGTACTTATT CTCGTGAGCC GCTTAAGCAA
    TCATTAATTA ACACTGAGTG TGATTCCTCT GATGCTATGA ACTCGTTCGA TTCTAATTTT
    GGTGCAGAAC GTGCTAATAC ATCTGACTTA TCCAGGGGAT TTGTCGCTTT TGCTAACCGT
    AAGAAATCTA AATACAATCT TCCTGGCTCT TCAGGATTTA TGACTCGATC CACTAAATCA
    ACTAATCCCA TGACTCCATT AAATTGGCTA CGGGGAATTT CTATGGGTCG GTTAGGTAAT
    GCTGATTGGG AAGTTCCTCA AAACCTTGGA GAAGAACTTT CTTGGATTGG GCAGAAGTAT
    TCCAACGTTA GCTTTGAGAA AATTGATGTA GCGGAAAGGA CTTGCACAAT ATCTTTAAAT
    GCTCCCATTC TTCCTGATGA CGGTTATGCT TATATTAGGT TGCATGTGTA TTTTCCAAAT
    AATTATCCTA TATCTGCAAC TCCAGTGTTT CAACTAGAAA GATCAAGCGC CTTTAATGAT
    GAGCAATTTA ATTATGTTTT TAATACATTA ACATCAATTT CAGACCAATG TATTTCTTCC
    CATAAATATT GCATGGATGC ATGCCTATCT TATCTGTCCG GCAATCTAAG TGTCGATGAA
    ATATGGAAGC TAGGCTTTCA AAAAGATAAT TCTGATTCAT CTTCTGAGTC CTCTGCCGAT
    ATTTTCCAAG ATGTTTTTCC TTCTATGCCC GATTTTCGAG GTGGAGATCG CGGCCTCTCT
    CATAAGCATC AAAATATTCC TCTTCCTAAA ACTTGTGCAG CTATATTTTG TGGTAATGAT
    GAGTTGGTGT GCTTTTTCAC AATCAAAGCC GATGAAAGTG CGGCACAGGC AACTGCCAAC
    CGCGAAACCC ACGGGCGCCA AAAGCTGTTT GAGTCATTTG GTGTTTTAGA TAGTAGTAAC
    AGCGTGGCAG ATTCAGACTC AACAAATTAC GATGACGAGA ACTCTTTAAA TAGAGGTGGC
    ACATCTGAAT CGGATAGTGA GTTTATTTGG GACGTTGATG AATCTAATTC AGGTTCAATT
    GTTTTCCCTA AATCCAAAAC CAGCATAAAC CTAAACAATA TAAACTCTGC ATCTGCATCT
    ATTATGGGCT CCAGATTTGG GGCTGCTGAC ATCTTCATGA GTAAACGCCC GTCGACAAAA
    GGTTCTAATA GACCCTCAAT TTTACTTTCT AAACCTTCTC ATGATTTTCA TGTTGTGAGG
    ATTATTAGTA TGAGCAAATA TCTCCCAGTG AAAAGAGCAT TGGCTGCTGA ATATGTTGTT
    GATACTGGAG ATAAAGTTAC AGTATGCAAC AAAAATGCTG AGGTATCTGC TAAGCATAAT
    TATTATCGCC TGGCAAAAGT TTGGTTAATG TTAGGTAGAC TTCTTGGTCA TTTATCACGC
    ACTGAAAACA AAGACCACAT TAATGACGAG GAAGCATTTC CATGGCTTAA GAGCCCTTTA
    GCAAAGTGGG TGGTTAATTC TTTACTGGAT TATTATGCTT CTCAATGCAA TACCCAAATG
    CTAGCTATGC TTGCTTGTGT TTTGGACATT CCTAATCCTA AGAAACAGTC AGGTAATACG
    TCTGCTGATC AAGTTCTTTT CAATCAACCT AAACAAGTTA CCGAAAAACT AGGTGTGCAG
    CTCAACCTCC CAAAGACCAA AATATTAGAA AAGGTATCTA GCCATTCATT AGCACAGATT
    ACAGCATTAC GAGAAAAAGA ACCAAAGGAT GCAGATTCTA CATATTCTAA AGAAAAGATT
    TTTTCCGCAA CCAGTACGGT ACATTTACAG CTGATGGATT ATGATAAACA AAACAACGAC
    TTTGTTGATG CACAAGAAAT TGCATATACC AAGTTATTAC AACAGAAATT CATACAATGG
    CGTGCCACGT ATGCTGAACA GCTTGATTTA TGGGGATTTT TCATACCAAA GTTAGAGATG
    CTAAAGTTTA ACGCTCACGA ATTTTCCTCA CCAAGTGAAA AGACATTAGT AAATCATTGC
    AATAGCTGCA ATTCCACGAC TTCTAATACT CGTATCTGCG AGAAATGCTA TAGCCTGGTA
    CCCCGTATGT CTTGCACATT TTGTTGTTTG TCGATTCACG GTTTATGCAT CGTATGCGGA
    CTATGCCTTC ATGTTATGCA TGAAGATTGT TATAAGGAGT GGTTTTCTAA CGGTGATTCT
    ATATCACAGT CGTGTTCTAG CGGTTGTGGT TGCAAATGCC AATTTCAGCA TATGTCTTTG
    GAAAAAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_594930.1
    CDS148..4119
    Translation

    Target ORF information:

    RefSeq Version NM_001020361.2
    Organism Schizosaccharomyces pombe(fission yeast)
    Definition Schizosaccharomyces pombe ubiquitin-protein ligase E3 (SPAC11E3.05), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001020361.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    ATGAGGGAAT TACAGGGAGA CGATTCTTCA AGAAAGTCTC CTCCGAGTGA TTCGGTTGTA 
    AAAAATTCTT CGCCTATTGA TTATGAACAT TCATTGAAAT CTCTTCAAGA TGAACGTACC
    TTGAATTACC CCAATAAGCA ATTTAATTCT GAAAACCCAT CTTCATACTA TAATATGGAT
    GATAGCGGGG AATTATGCAA CTTTGAATTG GAGAAAGACA GTCTTGAATC AATGATCCAT
    GAATCGTCCA CTGCTCTAAG CGCTCAATCA AACACTGCAC AAGACGGTGA TCAACTAGCT
    TCATCGTCAA CAATCTCTAA AGATCACTCA GAAACGTTGG ACAATAAGCT AAACGATTCT
    AAGATATTAA TTAAAAAAGC TTCTAACTCC TTTGCAAATT TTTCAGAAAG TGTAAGGTCT
    AGTACCATTG TTGCATATTC TCAAACACCG ACACAAAGGT TGCAAGGTTT AACTTCCATA
    TCATCTTCTT CTTTTGACGA TGGTAGTTAT GGATCTCGTC GAATATCTTT TAACTCTCAG
    GGTGTTTCTG GTCGGTTGCG AAGAAATATG GATTCCACGG TCATCATCCC TTCCGAAGAT
    GAAGACTTGC AGTTACCATC GAATTCTAAC AGCAATGTGG AATACGGCCC TTTTGATAGT
    ACTACGTTTG ATAGACAATT ATCAATTGAA GTTAATCAAC CAGTGGGTGC TATGTCTTTA
    TCTCCTTGTG GTAGAGATAT AGCTTTAGCT TCACGTTATG GTTTACTAGT CTTAGATCTT
    GATAATCCTT ATAATCCACC AAGAATGCTA AGACATTCTA CTCCTTGGGA AGTTGCTGAT
    ACTCAGTGGA ATGTTCATGC CGCAAGAGAC CAATGGGTGG TTTCTACATC AAGTCAAAAA
    ACGATAGTAT GGAACCTTGC ACTACCAAAT GATCGTGCTA TAGAATTTAT GTTACATGGA
    CACACCCGCG CCGTCACAGA TATGAACTGG CATCGGCAAA ATCCTGATGT TCTTGCCACT
    TGTTCAATTG ATTCCTCTGT TCACTGTTGG GATTTGCGTA GTCCTCGATT TCCTGTTAAC
    TCCTTTTACG ATTGGCATAA CGGAGCTACT CAGGTAAAAT GGAATTATAA AAATCCCCAC
    ATACTTGCTT CTAGCCATGG ACGTTTAGTA AGGATTTGGG ACGATCGTTA TGGATCCGCC
    CCACTTCATA CAATTAAAAC TTCCGAAAAT ATTACTAAAA TTAATGGTCT TGAATTCAAT
    AGAGCATGTG AAACTCGCCT ATTAACTTGT GCTATGGATC GAACAGTTAA GTTTTGGAAC
    TATGAAAAGT CAACAGAGGA GCCTGAGCAT TTAATTACCA CTGATTCTCC AGTATGGAAA
    GCAAGGTTTA CTCCATTCGG GGACGGAGTT ATTTTGATGC CTCAGAGAGG AGACAATTCC
    GTACACATGT ACGATTGTCG TAATTTAGAC AAAGAAGGCC CCCGTGCTGT ACATAGATTT
    GCCGGTCATA CCGATCAAGT TAAAGAATTT CTTTGGAGGT GTCGAGGCGA AGATGTATTT
    GATAGAGATT TAAGAGATTT TCAATTGATC ACTTGGTCCA AGGATCACCA TGTTCGTTTA
    TGGCCTATTG GTAACGATAT ACTCAATTCA ATGGGACACG ATCGAACAAA ACCTGTTCCT
    TTTAAATTAA CACGGTTAGG CGCGAAATAT CGTACTTATT CTCGTGAGCC GCTTAAGCAA
    TCATTAATTA ACACTGAGTG TGATTCCTCT GATGCTATGA ACTCGTTCGA TTCTAATTTT
    GGTGCAGAAC GTGCTAATAC ATCTGACTTA TCCAGGGGAT TTGTCGCTTT TGCTAACCGT
    AAGAAATCTA AATACAATCT TCCTGGCTCT TCAGGATTTA TGACTCGATC CACTAAATCA
    ACTAATCCCA TGACTCCATT AAATTGGCTA CGGGGAATTT CTATGGGTCG GTTAGGTAAT
    GCTGATTGGG AAGTTCCTCA AAACCTTGGA GAAGAACTTT CTTGGATTGG GCAGAAGTAT
    TCCAACGTTA GCTTTGAGAA AATTGATGTA GCGGAAAGGA CTTGCACAAT ATCTTTAAAT
    GCTCCCATTC TTCCTGATGA CGGTTATGCT TATATTAGGT TGCATGTGTA TTTTCCAAAT
    AATTATCCTA TATCTGCAAC TCCAGTGTTT CAACTAGAAA GATCAAGCGC CTTTAATGAT
    GAGCAATTTA ATTATGTTTT TAATACATTA ACATCAATTT CAGACCAATG TATTTCTTCC
    CATAAATATT GCATGGATGC ATGCCTATCT TATCTGTCCG GCAATCTAAG TGTCGATGAA
    ATATGGAAGC TAGGCTTTCA AAAAGATAAT TCTGATTCAT CTTCTGAGTC CTCTGCCGAT
    ATTTTCCAAG ATGTTTTTCC TTCTATGCCC GATTTTCGAG GTGGAGATCG CGGCCTCTCT
    CATAAGCATC AAAATATTCC TCTTCCTAAA ACTTGTGCAG CTATATTTTG TGGTAATGAT
    GAGTTGGTGT GCTTTTTCAC AATCAAAGCC GATGAAAGTG CGGCACAGGC AACTGCCAAC
    CGCGAAACCC ACGGGCGCCA AAAGCTGTTT GAGTCATTTG GTGTTTTAGA TAGTAGTAAC
    AGCGTGGCAG ATTCAGACTC AACAAATTAC GATGACGAGA ACTCTTTAAA TAGAGGTGGC
    ACATCTGAAT CGGATAGTGA GTTTATTTGG GACGTTGATG AATCTAATTC AGGTTCAATT
    GTTTTCCCTA AATCCAAAAC CAGCATAAAC CTAAACAATA TAAACTCTGC ATCTGCATCT
    ATTATGGGCT CCAGATTTGG GGCTGCTGAC ATCTTCATGA GTAAACGCCC GTCGACAAAA
    GGTTCTAATA GACCCTCAAT TTTACTTTCT AAACCTTCTC ATGATTTTCA TGTTGTGAGG
    ATTATTAGTA TGAGCAAATA TCTCCCAGTG AAAAGAGCAT TGGCTGCTGA ATATGTTGTT
    GATACTGGAG ATAAAGTTAC AGTATGCAAC AAAAATGCTG AGGTATCTGC TAAGCATAAT
    TATTATCGCC TGGCAAAAGT TTGGTTAATG TTAGGTAGAC TTCTTGGTCA TTTATCACGC
    ACTGAAAACA AAGACCACAT TAATGACGAG GAAGCATTTC CATGGCTTAA GAGCCCTTTA
    GCAAAGTGGG TGGTTAATTC TTTACTGGAT TATTATGCTT CTCAATGCAA TACCCAAATG
    CTAGCTATGC TTGCTTGTGT TTTGGACATT CCTAATCCTA AGAAACAGTC AGGTAATACG
    TCTGCTGATC AAGTTCTTTT CAATCAACCT AAACAAGTTA CCGAAAAACT AGGTGTGCAG
    CTCAACCTCC CAAAGACCAA AATATTAGAA AAGGTATCTA GCCATTCATT AGCACAGATT
    ACAGCATTAC GAGAAAAAGA ACCAAAGGAT GCAGATTCTA CATATTCTAA AGAAAAGATT
    TTTTCCGCAA CCAGTACGGT ACATTTACAG CTGATGGATT ATGATAAACA AAACAACGAC
    TTTGTTGATG CACAAGAAAT TGCATATACC AAGTTATTAC AACAGAAATT CATACAATGG
    CGTGCCACGT ATGCTGAACA GCTTGATTTA TGGGGATTTT TCATACCAAA GTTAGAGATG
    CTAAAGTTTA ACGCTCACGA ATTTTCCTCA CCAAGTGAAA AGACATTAGT AAATCATTGC
    AATAGCTGCA ATTCCACGAC TTCTAATACT CGTATCTGCG AGAAATGCTA TAGCCTGGTA
    CCCCGTATGT CTTGCACATT TTGTTGTTTG TCGATTCACG GTTTATGCAT CGTATGCGGA
    CTATGCCTTC ATGTTATGCA TGAAGATTGT TATAAGGAGT GGTTTTCTAA CGGTGATTCT
    ATATCACAGT CGTGTTCTAG CGGTTGTGGT TGCAAATGCC AATTTCAGCA TATGTCTTTG
    GAAAAAGTTT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

  • PubMed

    The genome sequence of Schizosaccharomyces pombe.
    Nature415(6874)871-80(2002 Feb)
    Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L