Wdr59 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene
Clones
gRNAs

Summary

Gene Symbol	Wdr59
Entrez Gene ID	34540
Full Name	WD repeat domain 59
Synonyms	CG4705,Dmel\CG4705,FBtr0080196,SEA3,Sea3
General protein information	Preferred Names WD repeat domain 59 Names CG4705 gene product from transcript CG4705-RD CG4705-PB CG4705-PD Wdr59-PB Wdr59-PD
Gene Type	protein-coding
Organism	Drosophila melanogaster(fruit fly)
Genome	Chromosome: 2L Map Location: 32D2-32D3

mRNA and Protein(s)

mRNA	Protein	Name
NM_205960.4	NP_995682.1	WD repeat domain 59, isoform B
NM_135641.4	NP_609485.4	WD repeat domain 59, isoform D

Pathways from BioSystems

Homologies

Homo sapiens (human)	WDR59	NP_085058.3
Saccharomyces cerevisiae (baker's yeast)	MTC5	NP_010413.3
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008331	XP_317129.4
Kluyveromyces lactis	KLLA0F19734g	XP_455966.1
	WDR59	XP_001109464.1
Rattus norvegicus (Norway rat)	Wdr59	XP_006255704.1
Gallus gallus (chicken)	WDR59	NP_001012794.1
Schizosaccharomyces pombe (fission yeast)	SPAC11E3.05	NP_594930.1
Bos taurus (cattle)	WDR59	NP_001094600.1
Xenopus tropicalis (tropical clawed frog)	wdr59	XP_004914115.1
Pan troglodytes (chimpanzee)	WDR59	XP_511107.2
Canis lupus familiaris (dog)	WDR59	XP_536785.3
Mus musculus (house mouse)	Wdr59	NP_001164214.1
Drosophila melanogaster (fruit fly)	CG4705	NP_995682.1
Eremothecium gossypii	AGOS_AGR067W	NP_986732.2

Gene Ontology
Bibliography

Related articles in PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
Matthews BB, Dos Santos G, Crosby MA, Emmert DB, St Pierre SE, Gramates LS, Zhou P, Schroeder AJ, Falls K, Strelets V, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
Crosby MA, Gramates LS, Dos Santos G, Matthews BB, St Pierre SE, Zhou P, Schroeder AJ, Falls K, Emmert DB, Russo SM, Gelbart WM,
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Hoskins RA, Carlson JW, Kennedy C, Acevedo D, Evans-Holm M, Frise E, Wan KH, Park S, Mendez-Lago M, Rossi F, Villasante A, Dimitri P, Karpen GH, Celniker SE
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Smith CD, Shu S, Mungall CJ, Karpen GH
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)

Combined evidence annotation of transposable elements in genome sequences.
Quesneville H, Bergman CM, Andrieu O, Autard D, Nouaud D, Ashburner M, Anxolabehere D
PLoS computational biology1(2)166-75(2005 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following Wdr59 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Wdr59 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OFa16652	NM_135641.4 Latest version!	Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant D, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
OFa16652	NM_205960.4 Latest version!	Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant B, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

*Business Day

** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Wdr59 ( NM_205960.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_995682.1 Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant B, mRNA.

Price & Availability↑

CloneID	OFa16652
Clone ID Related Accession (Same CDS sequence)	NM_205960.4 , NM_135641.4
Accession Version	NM_205960.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	WD repeat domain 59, isoform B
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_205960.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_995682.1
CDS	124..3033
Translation	MPPTETLRPGERGTAGGPGAGAPEQTYIVRQSNKYYEHRDSQATAMSVDYSGQWVLLAGRGHLALQRLGQDDGSLRRHERQSKYEVSVAEFAICPSRKEYCAIATSQHIDIVRWGTAEPHYEMSLRGHTRTVTDIDWHGKDPNLLVSCSIDTFSHIWDLREPRKPALSLNAVCMSGATQVGFNRVSGNLLAAAHDGDLRIWDIRKGSCPTHYITAHLNRVHGINWSHKRETCLATASQDGTVKYFDVCNPRRAEKIITTMSPVWRARYTPIGNGLVSIVVPHLGRGENSLLLWSNSKQTDPICSFVGHTDVILDFAWRPNRESSNEIELVTWSRDRTLRVWKIDDNMLKLCEPSAEEIESRFEPDLSELRVPTPPEFLHPRSILVAASLPISTGDGACNTLPMARSPSFGGGYYRREEPHIARSLTDQPTCSLHHEFSLLNTNMPHVEVDTLDAIKRYACFKICAGGHTVILQVTFTTSYPSPSAPPDFQLCQGTTLSSEVSGVLLKVLRCNALQRVKKSRTCLEQCLRALVAAMKKKVAAVGGADRSQLLLQSPRLEGALSSTLHDACIPYPRTSGVHFNAIGMLTTFAQPVNNKRLTLRQHTALTPRTFSSINGSGLLGNVMATAQREANASFYLQERMISGKPGKQRAIRQMNGSPVVHVYDTSSLLHISRRMAREFSLDKCNIAETCRKNGEICRQHGRLDLVPVWLLAELIATPQIPHETLNDLLFYKDPFKKSLLESLIMHYAISGDIQTAVLLACLFDKCPTGGGAIMEKTVRLPPQLNSQISPYHTVLPLDVKSSSSSNTWQQLKQLRSNSWSDSLDLEIKQIQSDAYACSLIRRTKMPLFDQFKRAYAEILFGWQLLSKRALILKHTQNTPPPVQGVEFVTECRKCAKPKRTPKCEPCKRPVLFCVLCRLPVKGAANACLACGHGGHIDHMMQWFEKHNVCATCGCKCLERTSELLALLS

Target ORF information:

RefSeq Version	NM_205960.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant B, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_205960.4

ORF Insert Sequence:

ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Wdr59 ( NM_135641.4 ) cDNA ORF clone, Drosophila melanogaster(fruit fly) -> NP_609485.4 Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant D, mRNA.

Price & Availability↑

CloneID	OFa16652
Clone ID Related Accession (Same CDS sequence)	NM_205960.4 , NM_135641.4
Accession Version	NM_135641.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-04-24
Organism	Drosophila melanogaster(fruit fly)
Product	WD repeat domain 59, isoform D
Comment	Comment: REVIEWED REFSEQ: This record has been curated by FlyBase. This record is derived from an annotated genomic sequence (NT_033779). On Jul 15, 2014 this sequence version replaced NM_135641.3. ##Genome-Annotation-Data-START## Annotation Provider :: FlyBase Annotation Status :: Full annotation Annotation Version :: Release 6.26 URL :: http://flybase.org ##Genome-Annotation-Data-END##

ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_609485.4
CDS	124..3033
Translation	MPPTETLRPGERGTAGGPGAGAPEQTYIVRQSNKYYEHRDSQATAMSVDYSGQWVLLAGRGHLALQRLGQDDGSLRRHERQSKYEVSVAEFAICPSRKEYCAIATSQHIDIVRWGTAEPHYEMSLRGHTRTVTDIDWHGKDPNLLVSCSIDTFSHIWDLREPRKPALSLNAVCMSGATQVGFNRVSGNLLAAAHDGDLRIWDIRKGSCPTHYITAHLNRVHGINWSHKRETCLATASQDGTVKYFDVCNPRRAEKIITTMSPVWRARYTPIGNGLVSIVVPHLGRGENSLLLWSNSKQTDPICSFVGHTDVILDFAWRPNRESSNEIELVTWSRDRTLRVWKIDDNMLKLCEPSAEEIESRFEPDLSELRVPTPPEFLHPRSILVAASLPISTGDGACNTLPMARSPSFGGGYYRREEPHIARSLTDQPTCSLHHEFSLLNTNMPHVEVDTLDAIKRYACFKICAGGHTVILQVTFTTSYPSPSAPPDFQLCQGTTLSSEVSGVLLKVLRCNALQRVKKSRTCLEQCLRALVAAMKKKVAAVGGADRSQLLLQSPRLEGALSSTLHDACIPYPRTSGVHFNAIGMLTTFAQPVNNKRLTLRQHTALTPRTFSSINGSGLLGNVMATAQREANASFYLQERMISGKPGKQRAIRQMNGSPVVHVYDTSSLLHISRRMAREFSLDKCNIAETCRKNGEICRQHGRLDLVPVWLLAELIATPQIPHETLNDLLFYKDPFKKSLLESLIMHYAISGDIQTAVLLACLFDKCPTGGGAIMEKTVRLPPQLNSQISPYHTVLPLDVKSSSSSNTWQQLKQLRSNSWSDSLDLEIKQIQSDAYACSLIRRTKMPLFDQFKRAYAEILFGWQLLSKRALILKHTQNTPPPVQGVEFVTECRKCAKPKRTPKCEPCKRPVLFCVLCRLPVKGAANACLACGHGGHIDHMMQWFEKHNVCATCGCKCLERTSELLALLS

Target ORF information:

RefSeq Version	NM_135641.4
Organism	Drosophila melanogaster(fruit fly)
Definition	Drosophila melanogaster WD repeat domain 59 (Wdr59), transcript variant D, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_135641.4

ORF Insert Sequence:

ATGCCGCCCA CGGAGACACT GCGTCCGGGA GAACGCGGAA CAGCCGGTGG ACCGGGAGCA 
GGAGCACCCG AACAAACGTA CATCGTCAGG CAGAGCAACA AATACTATGA ACACCGAGAT 
TCCCAGGCCA CGGCCATGTC GGTTGATTAC TCGGGTCAGT GGGTGCTCCT CGCGGGCCGT 
GGTCACCTGG CTTTGCAGCG TTTGGGACAG GACGATGGGT CTCTGCGACG ACATGAACGG 
CAATCGAAGT ACGAGGTGTC CGTAGCAGAG TTTGCCATAT GTCCCAGTCG CAAAGAGTAC 
TGCGCCATAG CGACTAGCCA GCACATAGAC ATCGTTCGCT GGGGCACTGC GGAACCACAC 
TACGAGATGT CCTTGCGTGG GCATACTCGA ACAGTGACCG ACATTGACTG GCACGGCAAG 
GATCCGAATC TGCTGGTCAG CTGCTCAATA GACACATTTT CTCATATTTG GGACTTAAGG 
GAGCCCAGGA AACCAGCCCT GTCCTTGAAT GCCGTGTGCA TGTCTGGTGC TACACAAGTG 
GGATTCAACC GCGTTTCGGG CAACCTTTTG GCTGCTGCCC ATGATGGAGA TTTACGCATC 
TGGGATATTC GCAAGGGTAG TTGCCCGACG CACTATATCA CAGCACATTT AAATAGAGTG 
CATGGCATCA ATTGGAGCCA CAAAAGGGAG ACCTGCTTGG CAACCGCCAG TCAGGATGGA 
ACGGTCAAGT ACTTTGATGT ATGTAATCCT CGCAGGGCCG AGAAGATCAT TACAACCATG 
TCGCCCGTTT GGAGAGCGAG ATACACGCCT ATTGGCAACG GTCTGGTTAG CATTGTGGTG 
CCCCATTTGG GACGCGGAGA GAATAGTTTG CTGCTCTGGA GCAACAGCAA GCAAACGGAC 
CCTATTTGTT CCTTCGTGGG GCACACCGAT GTCATTCTTG ACTTTGCCTG GCGTCCAAAT 
CGTGAAAGTT CCAATGAAAT AGAACTGGTC ACTTGGTCCA GGGATCGCAC GCTGCGGGTG 
TGGAAGATCG ATGATAATAT GCTAAAGCTT TGCGAACCGT CTGCGGAAGA AATAGAATCC 
CGTTTCGAGC CCGATCTCAG TGAGTTAAGA GTGCCTACTC CTCCAGAATT CCTGCATCCA 
CGATCAATTT TGGTTGCCGC TTCGTTGCCC ATATCCACGG GTGACGGTGC TTGTAATACC 
TTGCCCATGG CTCGCTCCCC GAGCTTTGGA GGTGGCTACT ATCGCAGGGA AGAGCCCCAC 
ATAGCCAGAT CACTAACAGA TCAACCCACG TGTTCGCTGC ACCACGAATT CTCGCTGCTC 
AACACAAACA TGCCTCATGT GGAGGTAGAC ACACTAGATG CCATAAAGCG GTACGCCTGC 
TTCAAGATTT GCGCCGGTGG ACACACGGTT ATTTTACAAG TGACTTTCAC GACATCGTAT 
CCAAGTCCTA GTGCTCCGCC GGACTTTCAG CTTTGCCAGG GAACCACTCT GTCCAGTGAA 
GTTAGTGGAG TTCTGCTAAA AGTGCTCCGT TGCAATGCAT TGCAACGGGT CAAGAAATCC 
CGCACGTGCC TGGAACAATG CCTACGTGCT CTAGTGGCGG CTATGAAGAA AAAGGTGGCC 
GCTGTAGGCG GAGCGGATCG TAGCCAACTG CTCCTGCAAT CCCCTCGGCT GGAAGGAGCT 
TTGTCGAGCA CCCTGCACGA TGCCTGCATT CCATATCCGC GGACGTCAGG GGTCCATTTC 
AATGCCATTG GTATGCTGAC CACTTTCGCC CAACCAGTGA ACAACAAAAG GCTGACCCTG 
CGGCAACACA CCGCATTGAC TCCACGGACT TTTTCATCGA TTAATGGTAG TGGATTGCTA 
GGCAATGTGA TGGCCACCGC CCAGAGGGAA GCTAACGCCT CCTTTTACCT CCAGGAGAGG 
ATGATTTCGG GAAAACCGGG CAAGCAGCGA GCCATCCGAC AGATGAATGG AAGTCCGGTG 
GTTCACGTAT ACGACACAAG TAGCTTACTC CACATTAGTC GGAGAATGGC TCGGGAGTTC 
TCCCTCGATA AGTGCAACAT AGCAGAAACG TGTCGTAAAA ATGGTGAGAT CTGTCGCCAG 
CATGGGCGCT TGGACTTGGT GCCCGTGTGG CTATTGGCGG AACTGATAGC CACACCCCAA 
ATTCCACATG AAACACTCAA CGATCTGCTG TTCTACAAAG ATCCCTTCAA GAAATCTCTG 
CTGGAATCAC TAATAATGCA TTATGCTATC TCCGGTGACA TACAGACTGC TGTGCTGCTG 
GCTTGTTTGT TTGACAAGTG CCCCACGGGT GGCGGTGCCA TTATGGAGAA GACAGTTCGA 
CTTCCGCCGC AGCTTAACTC GCAAATTTCA CCGTATCACA CGGTCCTGCC GCTTGACGTC 
AAGTCATCTT CTTCATCGAA CACTTGGCAG CAACTTAAGC AACTACGCAG CAACTCTTGG 
TCCGACTCCC TGGATTTGGA GATTAAGCAA ATCCAGTCTG ATGCATACGC TTGCTCATTG 
ATTAGACGGA CTAAGATGCC ACTGTTTGAT CAATTTAAGC GCGCCTATGC CGAAATTTTA 
TTTGGCTGGC AGTTGCTTTC GAAGCGGGCT TTGATCCTCA AGCACACGCA GAATACACCA 
CCTCCAGTCC AGGGTGTGGA ATTCGTTACC GAGTGCAGGA AGTGTGCCAA GCCCAAACGG 
ACGCCCAAAT GCGAGCCCTG CAAGCGACCC GTACTCTTCT GCGTCCTCTG CCGTTTGCCT 
GTGAAAGGAG CAGCCAATGC GTGTCTGGCT TGTGGCCATG GCGGTCACAT TGATCACATG 
ATGCAGTGGT TTGAGAAACA CAATGTCTGT GCTACGTGTG GCTGCAAGTG CTTGGAGCGC 
ACCTCAGAGC TGCTAGCCCT ACTCAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene Model Annotations for Drosophila melanogaster: Impact of High-Throughput Data.
G3 (Bethesda, Md.)5(8)1721-36(2015 Jun)
Matthews BB,Dos Santos G,Crosby MA,Emmert DB,St Pierre SE,Gramates LS,Zhou P,Schroeder AJ,Falls K,Strelets V,Russo SM,Gelbart WM,

Gene Model Annotations for Drosophila melanogaster: The Rule-Benders.
G3 (Bethesda, Md.)5(8)1737-49(2015 Jun)
Crosby MA,Gramates LS,Dos Santos G,Matthews BB,St Pierre SE,Zhou P,Schroeder AJ,Falls K,Emmert DB,Russo SM,Gelbart WM,

Sequence finishing and mapping of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1625-8(2007 Jun)
Hoskins RA,Carlson JW,Kennedy C,Acevedo D,Evans-Holm M,Frise E,Wan KH,Park S,Mendez-Lago M,Rossi F,Villasante A,Dimitri P,Karpen GH,Celniker SE

The Release 5.1 annotation of Drosophila melanogaster heterochromatin.
Science (New York, N.Y.)316(5831)1586-91(2007 Jun)
Smith CD,Shu S,Mungall CJ,Karpen GH

Combined evidence annotation of transposable elements in genome sequences.
PLoS computational biology1(2)166-75(2005 Jul)
Quesneville H,Bergman CM,Andrieu O,Autard D,Nouaud D,Ashburner M,Anxolabehere D

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Wdr59 cDNA ORF clone, Drosophila melanogaster(fruit fly)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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