ura1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ura1
Entrez Gene ID	2541544
Full Name	carbamoyl-phosphate synthase (glutamine hydrolyzing), aspartate carbamoyltransferase Ura1
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001018453.2	NP_593055.1	bifunctional carbamoyl-phosphate synthase/aspartate carbamoyltransferase Ura1

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	CAD	XP_001155357.1
Xenopus tropicalis (tropical clawed frog)	cad	XP_004920257.1
Canis lupus familiaris (dog)	CAD	XP_540124.3
Bos taurus (cattle)	CAD	NP_001178322.1
Gallus gallus (chicken)	CAD	XP_003641116.2
Kluyveromyces lactis	KLLA0E15357g	XP_454643.1
Eremothecium gossypii	AGOS_ACR263C	NP_983665.2
Neurospora crassa	NCU08287	XP_961813.1
Danio rerio (zebrafish)	cad	NP_001009884.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000300	XP_310823.5
Caenorhabditis elegans (roundworm)	pyr-1	NP_495838.2
Saccharomyces cerevisiae (baker's yeast)	URA2	NP_012405.2
Homo sapiens (human)	CAD	NP_004332.2
Rattus norvegicus (Norway rat)	Cad	NP_001099180.2
Drosophila melanogaster (fruit fly)	r	NP_523377.1
Schizosaccharomyces pombe (fission yeast)	ura1	NP_593055.1
	CAD	XP_002799221.1
Mus musculus (house mouse)	Cad	NP_076014.1
Magnaporthe oryzae (rice blast fungus)	MGG_01537	XP_003714510.1

Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ura1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ura1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc103614	NM_001018453.2 Latest version!	Schizosaccharomyces pombe bifunctional carbamoyl-phosphate synthase/aspartate carbamoyltransferase Ura1 (ura1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ura1 ( NM_001018453.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_593055.1 Schizosaccharomyces pombe bifunctional carbamoyl-phosphate synthase/aspartate carbamoyltransferase Ura1 (ura1), mRNA.

Price & Availability↑

CloneID	OSc103614
Clone ID Related Accession (Same CDS sequence)	NM_001018453.2
Accession Version	NM_001018453.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6735bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	bifunctional carbamoyl-phosphate synthase/aspartate carbamoyltransferase Ura1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003424). On Dec 10, 2012 this sequence version replaced NM_001018453.1.

ATGTCCGGAT TGCTACCTTC ATTGTCTTCA TCATTTCCAC TCGTCCAGAG TGAAGCTCTG 
GGGATGCCAA GGACTCATGG TCCTAAACCA TCTGAAAATG ATCCTAAAGA GCCCACCTGC 
TCTCCATCTC CTGCCTTCTA CTCTGTGAAT GGCGAGATGA AAGATTATAA GCTAATGGCC 
TTGGAATTGG AAGATAAAAG TGTTTTACAA GGTTATTCAT TCGGAGCTGA TCACTCTGTC 
TCCGGTGAAT TAGTTTTTCA GACTGGTATG GTCGGTTATC CTGAGTCTTT GACCGACCCT 
TCTTATCGTG GTCAGATTTT GGTCTTTACA TTTCCCACAG TGGGTAATTA TGGTGTGCCC 
GATCGTCGTA TTCTAGACGA GATATCTGGC TTACCCAAGT ATTTCGAATC CAATCAAATT 
CATGTTGCTG CTATTATTAT TTCATCTTAT TCTCAAAATT ATTCTCATTT TCTTGCTCAT 
TCTTCTTTAG GAGAATGGCT TAAGGAACAG GGCATTCCTG GTATTTTTGG TATTGATACC 
CGTGCTCTTA CTAAAAAAAT TCGCGACCAA GGTTCTATGT TAGGTCGTTT ACTTATTCAA 
AAAGACGAAT CCCCCATCAA TCCTTCTTCA ATTACTGGTC TCGGAAAAGA CTGGAGTACT 
GCTTTTGAAG ATATACCATG GAAAAATCCT AATACTGAAA ATTTAACGTC TCAAGTTTCC 
ATTAAAGAAC CCAAACTTTA TGAACCCCAT CCTACTACAG CTATCAAAAA AGCAGATGGA 
AAAATCATCC GTATCCTTGT TATTGATGTT GGTATGAAAT ATAACCAAAT TCGTTGTTTT 
TTGAATCGTG GTGTTGAATT ATTGGTTGTT CCTTGGGATT ATGATTTTAC CAAAGAGACT 
TATGATGGTC TATTTATTAG CAATGGACCT GGTGATCCTT CCCTCATGGA CTTGGTTGTT 
GATCGGGTAA AACGGGTGCT TGAGTCAAAG ACTGTCCCCG TTTTCGGTAT TTGTTTTGGA 
CATCAAATTA TGGCTCGAGC TGCCGGCGCC AGTACCACCA AAATGAAATT TGGTAACCGT 
GGCCACAATA TCCCTTGCAC TTGCATGATC TCGGGTAGAT GTTACATTAC TTCCCAAAAT 
CATGGTTATG CGGTTGATGC GTCTAGTTTA TCAAATGGCT GGAAAGAACT TTTTGTCAAT 
GCTAACGATG GTTCAAATGA AGGTATCTAT AATACTGAGT ATCCATTTTT CTCTGTGCAA 
TTTCACCCAG AGTCTACTCC TGGTCCCCGC GATACTGAAT TTCTCTTTGA TGTATTCATT 
GACGTTGTCA AGCGTTCAGC TGATGCAAAG TCTTTGCAGC CATTCAAGCT TCCTGGAGGA 
ACAATTGAAG AAAACAGATC CCGTCATCCA CTTGTGGATG CTAAGCGTGT TCTGATTTTG 
GGTAGTGGAG GATTGAGTAT TGGTCAAGCT GGTGAGTTTG ATTATTCTGG TAGTCAAGCT 
ATTAAGGCTC TTCGTGAAGA AGGAATTTAC ACCATTTTGA TTAATCCCAA CATTGCCACT 
ATTCAGACAT CTAAGGGTCT TGCTGATAAA GTGTACTTCT TACCTGTTAA TGCTGACTTT 
GTTCGTAAGG TGATCAAACA AGAGCGCCCC GATTCGATAT ATGTTACCTT TGGTGGTCAA 
ACAGCCCTGA ATGTTGGTAT TGAGCTGAAA GATGAGTTCG AACAACTCGG CGTTAAAGTT 
TTAGGTACGC CTATTGATAC CATTATTACC ACTGAGGACC GTGAACTTTT TGCTCGTGCA 
ATGGATGAAA TTAACGAGAA ATGTGCAAAA TCAGCTAGTG CTTCATCTAT TGAAGAAGCT 
ATAAAAGTAT CGAAGGATAT TTCTTTTCCA GTTATTGTCC GTGCTGCTTA TGCGCTTGGT 
GGACTTGGTT CAGGTTTTGC TGATAACGAG GCGGAACTCA TTGATTTATG TACGCTTGCT 
TTTGCTACCA GCCCTCAAGT CTTGATTGAA CGCAGTATGA AGGGATGGAA AGAAATTGAG 
TACGAAGTTG TTCGCGATGC TTTTGATAAC TGCATTACCG TCTGTAACAT GGAAAACTTT 
GATCCTCTCG GTATTCACAC TGGTGACTCT ATTGTTGTTG CTCCATCTCA AACTTTAACC 
GATGAAGATT ACAACATGCT TCGTACGACA GCTGTTAATG TTATTCGTCA CCTCGGTGTG 
GTGGGTGAAT GCAATATTCA GTATGCTTTG AACCCTTTTA CAAAAGAGTA TTGCATCATT 
GAAGTTAATG CTCGTTTGTC TCGTTCATCT GCTTTAGCGT CAAAGGCTAC CGGTTATCCT 
CTAGCTTTCA CAGCAGCAAA ACTAGGTCTT AACATTCCAC TTAACGAAGT CAAAAACTCA 
GTGACAAAGG TTACGTGCGC ATGCTTTGAA CCAAGTCTTG ATTATGTTGT CGTGAAGATT 
CCCCGTTGGG ACTTGAAGAA GTTTACTCGC GTTAGCACTC AGCTGAGCTC AGCAATGAAG 
TCAGTTGGTG AAGTGATGAG TATTGGACGT ACATTTGAGG AAGCAATCCA AAAGGCCATC 
AGGGCTATCG ATTATAGCAA TACTGGTTTC AACGTTAAGG ATGCTTTAAT GTCTATAGAT 
ACGGAATTGC AAACACCTTC GGACCAACGT CTGTTTGCTA TTGCCAACGC TATGGCTTCT 
GGTTATTCAG TGGACCGTAT TTGGGAGCTT ACTCGTATCG ATAAGTGGTT TCTTGAAAAG 
CTTATGGGAT TAATTCGTAC ATCCCAATTG ATTTCCAAGC ATGATATATC TAGTCTTCCT 
ATTTCTCTTC TAAAGACTGC CAAGCAATTA GGATTTGCGG ACGTCCAAAT TGCTGCTTTT 
ATGAATTCTA CTGAATTAGC CGTTAGACGT ATCCGTACTG AAGCTGGTAT CAGACCATTT 
GTAAAACAAA TTGATACAGT GGCTGCGGAG TTCCCAGCTT TCACCAACTA CCTTTATACC 
ACTTATAATG CTGTAGAGCA TGACATCCAT TTCAATGATA AGGGTGTGAT GGTTCTTGGC 
TCAGGTGTAT ATCGAATTGG CTCATCAGTT GAGTTTGACT GGTGCGCAGT TCGCGCAGTT 
CGTACGCTTC GTGATCGGGG AGTTAAAACT ATTATGGTTA ATTATAACCC AGAAACTGTT 
TCTACTGATT ATGATGAAGC CGATAGATTG TATTTCGAAA ATATCGGTCT TGAAACTGTC 
TTGGATATTT ACGAGCAAGA ATCATCTTCT GGTATCATTA TAGCTATGGG TGGTCAAACC 
GCTAATAACA TAGCCTTGCC ATTACATCGT GAAAATGTTA AAATTCTCGG TACTTCTCCT 
GAAATGATTG ATGGCGCCGA GAATAGATTT AAATTCTCTA GAATGCTGGA TGACATAGGT 
GTTGATCAGC CTAAGTGGAA GGAATTAACT AGTTTCGACG AGGCCGATAA ATTTTGCGAT 
ACAGTTGGTT ATCCTGTGTT AGTTCGTCCT TCTTATGTTC TTTCTGGTGC CGCAATGAAT 
ACAGTCTATT CTCAGTCAGA CCTGCATAGC TATTTGCAAC AAGCTGTTGC AATTAACAAA 
GATCATCCTG TTGTTATTTC TAAATATATT GAAAATGCTA AGGAAATCGA ATTAGATGCT 
GTTGCTCGTG AAGGAAAGAT GGTTATGCAC GTCATTTCCG AACATGTAGA AAACGCTGGT 
GTGCACTCTG GTGACGCTAC TTTGGTTTTA CCACCTCAAG ATTTGGCTCC AACGACTATA 
GAAAGAATTG TTGACGCCGC TGCTAAGATT GGAGAAGCTT TGAATATTAC TGGTCCTTAT 
AACATTCAAT TTATTGCGAA GGACAACGAA ATTAAAGTAA TTGAATGCAA TGTTCGTGCA 
TCGCGTTCAT TCCCTTTTGT TTCTAAGGTT ATAGGTGTTG ATATGATTTC AATGGCGACA 
GATGTCATTA TGGGTAATCC TATTCAACCC TACCCCGATG TTGATCTTCC TCGCGATTAC 
GTTGCTGTCA AAGTACCTCA ATTCAGTTTC AGCAGACTGT CCGGTGCTGA CCCCGTCCTT 
GGTGTCGAAA TGGCGTCTAC TGGTGAAGTT GCTTGTTTTG GTCACAATAA ATTTGAAGCC 
TATCTTAAAG CTATGATTTC CACCGGCTTT AGACTCCCGA AAAAGAATAT TCTTATATCT 
ATTGGTTCAT ATAAAGAAAA GGCGGAGCTT TTACCTTACG TCAAGAAGTT GTATGAAAAC 
AATTACAACA TTTTCGCAAC AGCCGGTACC TCAGACTACT TTATGGAAAG TGGTGTTCCT 
TGCAAGTACC TTGCAGATCT TCCTGCTGAA GAAGCAAATA ATGAGTATTC ACTTTCCGCT 
CATTTAGCCA ACAACATGAT TGACATGTAT ATCAATTTGC CCTCCAACAA TCGTTATCGT 
CGTCCTGCTA ATTATATTAG CAGTGGTTAT AAAAGTCGTC GTCTTGCCAT TGATTATTCA 
GTCCCTTTGG TTACAAATGT CAAATGTGCA AAGCTTATGA TTGAGGCAAT TTGCAGAAAC 
TTGGATTTCA GCTTGAGTAC AGTAGATTTC CAGAGCAGTT TCCAAACTGC TAATCTACCA 
GGTTTGATTA ACATATCTGC TTTCCTTCCT GAATTTACTT CTGAAGCCGT TTCTGACTAT 
ACAAAAGAAT GTATTGCATC TGGTTTTACT ATGGCTCGAT TTTTACCATT TAGTACGTCT 
TCTACTTTAG CAGACAAGGA TTCGTTGTCT GCTGTTAAAA AGTTAGCTTT AGACCATGCT 
CATTGCGATT ACAACTTTTC AGTCATTGCC TCCTCAACTA ATGAAGTTAC TATCTCTGAA 
CTCACATCTG AATCTGGCTG TTTGTTTTTC CATTTTGAGA AAGACGATTC TGGTATCGAT 
CATGTTACTG CAGTTGCCTC TCATTTTAAC GTATGGCCTG ATACGCAGAC TGTGATGACA 
GATGCCAAAT CGACAACTTT AGCTTCGTTA TTGTTATTGG CAAGTCTTCA TAACCGTAGG 
ATTCATATTA CAAATGTATC TTCTAAGGAC GATTTGAATT TGATTGTACT TGCTAAGCAA 
AGAAGTCTTC CTGTCACATT TGATGTTTCA GTTTATAGTC TTTTCTTGAA CCAAAATGAT 
TATCCCGGAG CGACATTTTT ACCTACTGCT GATGATCAGG TTGAGATATG GAATAAGCTC 
TCATACATTG ATTGTTTTTC TATTGGTAGT ATTCCTTCTC GTCTTGCTAA GTTCGTGGGT 
AATACGGCTT TTACTGGCTT TGGAGTTCGC GAGGCAATTC CACTTTTGTT AACAGCTGTT 
AATGAAGGTA GACTTACTTT AAAGGATGTT GTTGATCGCT TCTATTCGAA CCCTAAAGCC 
ATTTTCCGTT TGCATGATCA AGACGATTCT GGCGTTCAAT TGGAAGTTGA TCGTTCTGTT 
AGCTGGTCTT CCAAAGACTG GACCCCTTTC AATGGCAAGA AACTTTATGG TAGCATCCAA 
TCTGTTCAGT TTGACGGTCA TGATGTATTC TTTGATGGTG AACTGAATTT CGAACATACT 
TATGGTCGTG ACTATTCTTC GGCTAGTTTG GCTGACAAAT CTAAGGCAAC TAGAAAGGTT 
AGTGCGTTGA TGAGTCCTGG TCTTCCCCAT GCTGCCCCTT CTCTTGCCGA AGCATTCGGT 
CAAGCACCAG AGAACAAAGC GCATCCTGAT ATTAGCTTGA ACATGACTCC TAATTTCAAG 
CCTTCTCATG AACTGGTACA ATTGATCAAC TCTTCGCCCT TCTATCGCAA GCATATCATT 
TCGGTGCACC AAGTTACTCG TTCTGATTTG CATGTACTAT TTGCTATTGC TCATCAAATG 
AGAATCATAG TTGAAAGACA AGGTGTGATT GACTTGTGCT ATGGAAAACT TCTTTGTACT 
ATGTTCTTTG AACCATCAAC TCGTACTTCA TCGTCTTTTG ATGCTGCAAT GCAACGTTTA 
GGAGGAAAAG TAGTCGCTGT TACAGCTAGT GCTTCTAGTG TCAATAAAGG TGAGAGCTTG 
GCTGATACCA TTCGTACACT TGGATGTTAT GGTGATGCTA TTGTCTTGCG CCATCCTTCA 
ATTGAAAGCG CACGCATTGC TGCTAACTTT TCTCCCGTTC CTATCATCAA TGGTGGTAAT 
GGCTCGAAGG AGCATCCCAC ACAAGCTTTC TTGGATCTTT ATACCATTCG TGAAGAGTTA 
GGTAGTGTCA ATGGACTTAC AATTACGTTT ATTGGTGATC TTAAATACGG ACGTACCGTT 
CATTCATTAG CTCGTTTGCT TGCGTTTTGG CATGTCGAAT TGCATCTTGT ATCACCTGAA 
CAATTGGCAC TTCCTGACGA CGTGAAAGAC GATATTCGTG CGAATGGATT GAATTTCATT 
GAGCACCGTG AGTTGACTAA AGAAGTTGTT GCACAATCTG ATGTACTTTA CTGTACCAGA 
GTACAGAAAG AACGTTTTGC TAGTGTTGAT GAATATGAGA AATTAAAGGA TTCTTTTATC 
GTTGACAACA GTGTTCTAGC CTCTGCCAAA TCCCACTGTA TCGTTATGCA TCCTTTGCCT 
CGTAATCGTG AAATCAGTGA AGAGGTTGAT TTTGATCAAA GACGTGCTGC TTACTTCCGA 
CAAATGCGTT ATGGTCTCTA TATTCGTATG GCTTTACTCG CTTGTGTTAT GGGTGCTACT 
GAAGTAGCAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_593055.1
CDS	84..6818
Translation	MSGLLPSLSSSFPLVQSEALGMPRTHGPKPSENDPKEPTCSPSPAFYSVNGEMKDYKLMALELEDKSVLQGYSFGADHSVSGELVFQTGMVGYPESLTDPSYRGQILVFTFPTVGNYGVPDRRILDEISGLPKYFESNQIHVAAIIISSYSQNYSHFLAHSSLGEWLKEQGIPGIFGIDTRALTKKIRDQGSMLGRLLIQKDESPINPSSITGLGKDWSTAFEDIPWKNPNTENLTSQVSIKEPKLYEPHPTTAIKKADGKIIRILVIDVGMKYNQIRCFLNRGVELLVVPWDYDFTKETYDGLFISNGPGDPSLMDLVVDRVKRVLESKTVPVFGICFGHQIMARAAGASTTKMKFGNRGHNIPCTCMISGRCYITSQNHGYAVDASSLSNGWKELFVNANDGSNEGIYNTEYPFFSVQFHPESTPGPRDTEFLFDVFIDVVKRSADAKSLQPFKLPGGTIEENRSRHPLVDAKRVLILGSGGLSIGQAGEFDYSGSQAIKALREEGIYTILINPNIATIQTSKGLADKVYFLPVNADFVRKVIKQERPDSIYVTFGGQTALNVGIELKDEFEQLGVKVLGTPIDTIITTEDRELFARAMDEINEKCAKSASASSIEEAIKVSKDISFPVIVRAAYALGGLGSGFADNEAELIDLCTLAFATSPQVLIERSMKGWKEIEYEVVRDAFDNCITVCNMENFDPLGIHTGDSIVVAPSQTLTDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFTKEYCIIEVNARLSRSSALASKATGYPLAFTAAKLGLNIPLNEVKNSVTKVTCACFEPSLDYVVVKIPRWDLKKFTRVSTQLSSAMKSVGEVMSIGRTFEEAIQKAIRAIDYSNTGFNVKDALMSIDTELQTPSDQRLFAIANAMASGYSVDRIWELTRIDKWFLEKLMGLIRTSQLISKHDISSLPISLLKTAKQLGFADVQIAAFMNSTELAVRRIRTEAGIRPFVKQIDTVAAEFPAFTNYLYTTYNAVEHDIHFNDKGVMVLGSGVYRIGSSVEFDWCAVRAVRTLRDRGVKTIMVNYNPETVSTDYDEADRLYFENIGLETVLDIYEQESSSGIIIAMGGQTANNIALPLHRENVKILGTSPEMIDGAENRFKFSRMLDDIGVDQPKWKELTSFDEADKFCDTVGYPVLVRPSYVLSGAAMNTVYSQSDLHSYLQQAVAINKDHPVVISKYIENAKEIELDAVAREGKMVMHVISEHVENAGVHSGDATLVLPPQDLAPTTIERIVDAAAKIGEALNITGPYNIQFIAKDNEIKVIECNVRASRSFPFVSKVIGVDMISMATDVIMGNPIQPYPDVDLPRDYVAVKVPQFSFSRLSGADPVLGVEMASTGEVACFGHNKFEAYLKAMISTGFRLPKKNILISIGSYKEKAELLPYVKKLYENNYNIFATAGTSDYFMESGVPCKYLADLPAEEANNEYSLSAHLANNMIDMYINLPSNNRYRRPANYISSGYKSRRLAIDYSVPLVTNVKCAKLMIEAICRNLDFSLSTVDFQSSFQTANLPGLINISAFLPEFTSEAVSDYTKECIASGFTMARFLPFSTSSTLADKDSLSAVKKLALDHAHCDYNFSVIASSTNEVTISELTSESGCLFFHFEKDDSGIDHVTAVASHFNVWPDTQTVMTDAKSTTLASLLLLASLHNRRIHITNVSSKDDLNLIVLAKQRSLPVTFDVSVYSLFLNQNDYPGATFLPTADDQVEIWNKLSYIDCFSIGSIPSRLAKFVGNTAFTGFGVREAIPLLLTAVNEGRLTLKDVVDRFYSNPKAIFRLHDQDDSGVQLEVDRSVSWSSKDWTPFNGKKLYGSIQSVQFDGHDVFFDGELNFEHTYGRDYSSASLADKSKATRKVSALMSPGLPHAAPSLAEAFGQAPENKAHPDISLNMTPNFKPSHELVQLINSSPFYRKHIISVHQVTRSDLHVLFAIAHQMRIIVERQGVIDLCYGKLLCTMFFEPSTRTSSSFDAAMQRLGGKVVAVTASASSVNKGESLADTIRTLGCYGDAIVLRHPSIESARIAANFSPVPIINGGNGSKEHPTQAFLDLYTIREELGSVNGLTITFIGDLKYGRTVHSLARLLAFWHVELHLVSPEQLALPDDVKDDIRANGLNFIEHRELTKEVVAQSDVLYCTRVQKERFASVDEYEKLKDSFIVDNSVLASAKSHCIVMHPLPRNREISEEVDFDQRRAAYFRQMRYGLYIRMALLACVMGATEVAN

Target ORF information:

RefSeq Version	NM_001018453.2
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe bifunctional carbamoyl-phosphate synthase/aspartate carbamoyltransferase Ura1 (ura1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001018453.2

ORF Insert Sequence:

ATGTCCGGAT TGCTACCTTC ATTGTCTTCA TCATTTCCAC TCGTCCAGAG TGAAGCTCTG 
GGGATGCCAA GGACTCATGG TCCTAAACCA TCTGAAAATG ATCCTAAAGA GCCCACCTGC 
TCTCCATCTC CTGCCTTCTA CTCTGTGAAT GGCGAGATGA AAGATTATAA GCTAATGGCC 
TTGGAATTGG AAGATAAAAG TGTTTTACAA GGTTATTCAT TCGGAGCTGA TCACTCTGTC 
TCCGGTGAAT TAGTTTTTCA GACTGGTATG GTCGGTTATC CTGAGTCTTT GACCGACCCT 
TCTTATCGTG GTCAGATTTT GGTCTTTACA TTTCCCACAG TGGGTAATTA TGGTGTGCCC 
GATCGTCGTA TTCTAGACGA GATATCTGGC TTACCCAAGT ATTTCGAATC CAATCAAATT 
CATGTTGCTG CTATTATTAT TTCATCTTAT TCTCAAAATT ATTCTCATTT TCTTGCTCAT 
TCTTCTTTAG GAGAATGGCT TAAGGAACAG GGCATTCCTG GTATTTTTGG TATTGATACC 
CGTGCTCTTA CTAAAAAAAT TCGCGACCAA GGTTCTATGT TAGGTCGTTT ACTTATTCAA 
AAAGACGAAT CCCCCATCAA TCCTTCTTCA ATTACTGGTC TCGGAAAAGA CTGGAGTACT 
GCTTTTGAAG ATATACCATG GAAAAATCCT AATACTGAAA ATTTAACGTC TCAAGTTTCC 
ATTAAAGAAC CCAAACTTTA TGAACCCCAT CCTACTACAG CTATCAAAAA AGCAGATGGA 
AAAATCATCC GTATCCTTGT TATTGATGTT GGTATGAAAT ATAACCAAAT TCGTTGTTTT 
TTGAATCGTG GTGTTGAATT ATTGGTTGTT CCTTGGGATT ATGATTTTAC CAAAGAGACT 
TATGATGGTC TATTTATTAG CAATGGACCT GGTGATCCTT CCCTCATGGA CTTGGTTGTT 
GATCGGGTAA AACGGGTGCT TGAGTCAAAG ACTGTCCCCG TTTTCGGTAT TTGTTTTGGA 
CATCAAATTA TGGCTCGAGC TGCCGGCGCC AGTACCACCA AAATGAAATT TGGTAACCGT 
GGCCACAATA TCCCTTGCAC TTGCATGATC TCGGGTAGAT GTTACATTAC TTCCCAAAAT 
CATGGTTATG CGGTTGATGC GTCTAGTTTA TCAAATGGCT GGAAAGAACT TTTTGTCAAT 
GCTAACGATG GTTCAAATGA AGGTATCTAT AATACTGAGT ATCCATTTTT CTCTGTGCAA 
TTTCACCCAG AGTCTACTCC TGGTCCCCGC GATACTGAAT TTCTCTTTGA TGTATTCATT 
GACGTTGTCA AGCGTTCAGC TGATGCAAAG TCTTTGCAGC CATTCAAGCT TCCTGGAGGA 
ACAATTGAAG AAAACAGATC CCGTCATCCA CTTGTGGATG CTAAGCGTGT TCTGATTTTG 
GGTAGTGGAG GATTGAGTAT TGGTCAAGCT GGTGAGTTTG ATTATTCTGG TAGTCAAGCT 
ATTAAGGCTC TTCGTGAAGA AGGAATTTAC ACCATTTTGA TTAATCCCAA CATTGCCACT 
ATTCAGACAT CTAAGGGTCT TGCTGATAAA GTGTACTTCT TACCTGTTAA TGCTGACTTT 
GTTCGTAAGG TGATCAAACA AGAGCGCCCC GATTCGATAT ATGTTACCTT TGGTGGTCAA 
ACAGCCCTGA ATGTTGGTAT TGAGCTGAAA GATGAGTTCG AACAACTCGG CGTTAAAGTT 
TTAGGTACGC CTATTGATAC CATTATTACC ACTGAGGACC GTGAACTTTT TGCTCGTGCA 
ATGGATGAAA TTAACGAGAA ATGTGCAAAA TCAGCTAGTG CTTCATCTAT TGAAGAAGCT 
ATAAAAGTAT CGAAGGATAT TTCTTTTCCA GTTATTGTCC GTGCTGCTTA TGCGCTTGGT 
GGACTTGGTT CAGGTTTTGC TGATAACGAG GCGGAACTCA TTGATTTATG TACGCTTGCT 
TTTGCTACCA GCCCTCAAGT CTTGATTGAA CGCAGTATGA AGGGATGGAA AGAAATTGAG 
TACGAAGTTG TTCGCGATGC TTTTGATAAC TGCATTACCG TCTGTAACAT GGAAAACTTT 
GATCCTCTCG GTATTCACAC TGGTGACTCT ATTGTTGTTG CTCCATCTCA AACTTTAACC 
GATGAAGATT ACAACATGCT TCGTACGACA GCTGTTAATG TTATTCGTCA CCTCGGTGTG 
GTGGGTGAAT GCAATATTCA GTATGCTTTG AACCCTTTTA CAAAAGAGTA TTGCATCATT 
GAAGTTAATG CTCGTTTGTC TCGTTCATCT GCTTTAGCGT CAAAGGCTAC CGGTTATCCT 
CTAGCTTTCA CAGCAGCAAA ACTAGGTCTT AACATTCCAC TTAACGAAGT CAAAAACTCA 
GTGACAAAGG TTACGTGCGC ATGCTTTGAA CCAAGTCTTG ATTATGTTGT CGTGAAGATT 
CCCCGTTGGG ACTTGAAGAA GTTTACTCGC GTTAGCACTC AGCTGAGCTC AGCAATGAAG 
TCAGTTGGTG AAGTGATGAG TATTGGACGT ACATTTGAGG AAGCAATCCA AAAGGCCATC 
AGGGCTATCG ATTATAGCAA TACTGGTTTC AACGTTAAGG ATGCTTTAAT GTCTATAGAT 
ACGGAATTGC AAACACCTTC GGACCAACGT CTGTTTGCTA TTGCCAACGC TATGGCTTCT 
GGTTATTCAG TGGACCGTAT TTGGGAGCTT ACTCGTATCG ATAAGTGGTT TCTTGAAAAG 
CTTATGGGAT TAATTCGTAC ATCCCAATTG ATTTCCAAGC ATGATATATC TAGTCTTCCT 
ATTTCTCTTC TAAAGACTGC CAAGCAATTA GGATTTGCGG ACGTCCAAAT TGCTGCTTTT 
ATGAATTCTA CTGAATTAGC CGTTAGACGT ATCCGTACTG AAGCTGGTAT CAGACCATTT 
GTAAAACAAA TTGATACAGT GGCTGCGGAG TTCCCAGCTT TCACCAACTA CCTTTATACC 
ACTTATAATG CTGTAGAGCA TGACATCCAT TTCAATGATA AGGGTGTGAT GGTTCTTGGC 
TCAGGTGTAT ATCGAATTGG CTCATCAGTT GAGTTTGACT GGTGCGCAGT TCGCGCAGTT 
CGTACGCTTC GTGATCGGGG AGTTAAAACT ATTATGGTTA ATTATAACCC AGAAACTGTT 
TCTACTGATT ATGATGAAGC CGATAGATTG TATTTCGAAA ATATCGGTCT TGAAACTGTC 
TTGGATATTT ACGAGCAAGA ATCATCTTCT GGTATCATTA TAGCTATGGG TGGTCAAACC 
GCTAATAACA TAGCCTTGCC ATTACATCGT GAAAATGTTA AAATTCTCGG TACTTCTCCT 
GAAATGATTG ATGGCGCCGA GAATAGATTT AAATTCTCTA GAATGCTGGA TGACATAGGT 
GTTGATCAGC CTAAGTGGAA GGAATTAACT AGTTTCGACG AGGCCGATAA ATTTTGCGAT 
ACAGTTGGTT ATCCTGTGTT AGTTCGTCCT TCTTATGTTC TTTCTGGTGC CGCAATGAAT 
ACAGTCTATT CTCAGTCAGA CCTGCATAGC TATTTGCAAC AAGCTGTTGC AATTAACAAA 
GATCATCCTG TTGTTATTTC TAAATATATT GAAAATGCTA AGGAAATCGA ATTAGATGCT 
GTTGCTCGTG AAGGAAAGAT GGTTATGCAC GTCATTTCCG AACATGTAGA AAACGCTGGT 
GTGCACTCTG GTGACGCTAC TTTGGTTTTA CCACCTCAAG ATTTGGCTCC AACGACTATA 
GAAAGAATTG TTGACGCCGC TGCTAAGATT GGAGAAGCTT TGAATATTAC TGGTCCTTAT 
AACATTCAAT TTATTGCGAA GGACAACGAA ATTAAAGTAA TTGAATGCAA TGTTCGTGCA 
TCGCGTTCAT TCCCTTTTGT TTCTAAGGTT ATAGGTGTTG ATATGATTTC AATGGCGACA 
GATGTCATTA TGGGTAATCC TATTCAACCC TACCCCGATG TTGATCTTCC TCGCGATTAC 
GTTGCTGTCA AAGTACCTCA ATTCAGTTTC AGCAGACTGT CCGGTGCTGA CCCCGTCCTT 
GGTGTCGAAA TGGCGTCTAC TGGTGAAGTT GCTTGTTTTG GTCACAATAA ATTTGAAGCC 
TATCTTAAAG CTATGATTTC CACCGGCTTT AGACTCCCGA AAAAGAATAT TCTTATATCT 
ATTGGTTCAT ATAAAGAAAA GGCGGAGCTT TTACCTTACG TCAAGAAGTT GTATGAAAAC 
AATTACAACA TTTTCGCAAC AGCCGGTACC TCAGACTACT TTATGGAAAG TGGTGTTCCT 
TGCAAGTACC TTGCAGATCT TCCTGCTGAA GAAGCAAATA ATGAGTATTC ACTTTCCGCT 
CATTTAGCCA ACAACATGAT TGACATGTAT ATCAATTTGC CCTCCAACAA TCGTTATCGT 
CGTCCTGCTA ATTATATTAG CAGTGGTTAT AAAAGTCGTC GTCTTGCCAT TGATTATTCA 
GTCCCTTTGG TTACAAATGT CAAATGTGCA AAGCTTATGA TTGAGGCAAT TTGCAGAAAC 
TTGGATTTCA GCTTGAGTAC AGTAGATTTC CAGAGCAGTT TCCAAACTGC TAATCTACCA 
GGTTTGATTA ACATATCTGC TTTCCTTCCT GAATTTACTT CTGAAGCCGT TTCTGACTAT 
ACAAAAGAAT GTATTGCATC TGGTTTTACT ATGGCTCGAT TTTTACCATT TAGTACGTCT 
TCTACTTTAG CAGACAAGGA TTCGTTGTCT GCTGTTAAAA AGTTAGCTTT AGACCATGCT 
CATTGCGATT ACAACTTTTC AGTCATTGCC TCCTCAACTA ATGAAGTTAC TATCTCTGAA 
CTCACATCTG AATCTGGCTG TTTGTTTTTC CATTTTGAGA AAGACGATTC TGGTATCGAT 
CATGTTACTG CAGTTGCCTC TCATTTTAAC GTATGGCCTG ATACGCAGAC TGTGATGACA 
GATGCCAAAT CGACAACTTT AGCTTCGTTA TTGTTATTGG CAAGTCTTCA TAACCGTAGG 
ATTCATATTA CAAATGTATC TTCTAAGGAC GATTTGAATT TGATTGTACT TGCTAAGCAA 
AGAAGTCTTC CTGTCACATT TGATGTTTCA GTTTATAGTC TTTTCTTGAA CCAAAATGAT 
TATCCCGGAG CGACATTTTT ACCTACTGCT GATGATCAGG TTGAGATATG GAATAAGCTC 
TCATACATTG ATTGTTTTTC TATTGGTAGT ATTCCTTCTC GTCTTGCTAA GTTCGTGGGT 
AATACGGCTT TTACTGGCTT TGGAGTTCGC GAGGCAATTC CACTTTTGTT AACAGCTGTT 
AATGAAGGTA GACTTACTTT AAAGGATGTT GTTGATCGCT TCTATTCGAA CCCTAAAGCC 
ATTTTCCGTT TGCATGATCA AGACGATTCT GGCGTTCAAT TGGAAGTTGA TCGTTCTGTT 
AGCTGGTCTT CCAAAGACTG GACCCCTTTC AATGGCAAGA AACTTTATGG TAGCATCCAA 
TCTGTTCAGT TTGACGGTCA TGATGTATTC TTTGATGGTG AACTGAATTT CGAACATACT 
TATGGTCGTG ACTATTCTTC GGCTAGTTTG GCTGACAAAT CTAAGGCAAC TAGAAAGGTT 
AGTGCGTTGA TGAGTCCTGG TCTTCCCCAT GCTGCCCCTT CTCTTGCCGA AGCATTCGGT 
CAAGCACCAG AGAACAAAGC GCATCCTGAT ATTAGCTTGA ACATGACTCC TAATTTCAAG 
CCTTCTCATG AACTGGTACA ATTGATCAAC TCTTCGCCCT TCTATCGCAA GCATATCATT 
TCGGTGCACC AAGTTACTCG TTCTGATTTG CATGTACTAT TTGCTATTGC TCATCAAATG 
AGAATCATAG TTGAAAGACA AGGTGTGATT GACTTGTGCT ATGGAAAACT TCTTTGTACT 
ATGTTCTTTG AACCATCAAC TCGTACTTCA TCGTCTTTTG ATGCTGCAAT GCAACGTTTA 
GGAGGAAAAG TAGTCGCTGT TACAGCTAGT GCTTCTAGTG TCAATAAAGG TGAGAGCTTG 
GCTGATACCA TTCGTACACT TGGATGTTAT GGTGATGCTA TTGTCTTGCG CCATCCTTCA 
ATTGAAAGCG CACGCATTGC TGCTAACTTT TCTCCCGTTC CTATCATCAA TGGTGGTAAT 
GGCTCGAAGG AGCATCCCAC ACAAGCTTTC TTGGATCTTT ATACCATTCG TGAAGAGTTA 
GGTAGTGTCA ATGGACTTAC AATTACGTTT ATTGGTGATC TTAAATACGG ACGTACCGTT 
CATTCATTAG CTCGTTTGCT TGCGTTTTGG CATGTCGAAT TGCATCTTGT ATCACCTGAA 
CAATTGGCAC TTCCTGACGA CGTGAAAGAC GATATTCGTG CGAATGGATT GAATTTCATT 
GAGCACCGTG AGTTGACTAA AGAAGTTGTT GCACAATCTG ATGTACTTTA CTGTACCAGA 
GTACAGAAAG AACGTTTTGC TAGTGTTGAT GAATATGAGA AATTAAAGGA TTCTTTTATC 
GTTGACAACA GTGTTCTAGC CTCTGCCAAA TCCCACTGTA TCGTTATGCA TCCTTTGCCT 
CGTAATCGTG AAATCAGTGA AGAGGTTGAT TTTGATCAAA GACGTGCTGC TTACTTCCGA 
CAAATGCGTT ATGGTCTCTA TATTCGTATG GCTTTACTCG CTTGTGTTAT GGGTGCTACT 
GAAGTAGCAA ATTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ura1 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

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