ppk29 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene
Clones
gRNAs

Summary

Gene Symbol	ppk29
Entrez Gene ID	2541080
Full Name	Ark1/Prk1 family protein kinase Ppk29
Gene Type	protein-coding
Organism	Schizosaccharomyces pombe(fission yeast)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001021936.2	NP_596027.1	Ark1/Prk1 family protein kinase Ppk29
NM_001355774.1	NP_001342923.1	Ark1/Prk1 family protein kinase Ppk29

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

The genome sequence of Schizosaccharomyces pombe.
Wood V, Gwilliam R, Rajandream MA, Lyne M, Lyne R, Stewart A, Sgouros J, Peat N, Hayles J, Baker S, Basham D, Bowman S, Brooks K, Brown D, Brown S, Chillingworth T, Churcher C, Collins M, Connor R, Cronin A, Davis P, Feltwell T, Fraser A, Gentles S, Goble A, Hamlin N, Harris D, Hidalgo J, Hodgson G, Holroyd S, Hornsby T, Howarth S, Huckle EJ, Hunt S, Jagels K, James K, Jones L, Jones M, Leather S, McDonald S, McLean J, Mooney P, Moule S, Mungall K, Murphy L, Niblett D, Odell C, Oliver K, O'Neil S, Pearson D, Quail MA, Rabbinowitsch E, Rutherford K, Rutter S, Saunders D, Seeger K, Sharp S, Skelton J, Simmonds M, Squares R, Squares S, Stevens K, Taylor K, Taylor RG, Tivey A, Walsh S, Warren T, Whitehead S, Woodward J, Volckaert G, Aert R, Robben J, Grymonprez B, Weltjens I, Vanstreels E, Rieger M, Sch?fer M, M?ller-Auer S, Gabel C, Fuchs M, D?sterh?ft A, Fritzc C, Holzer E, Moestl D, Hilbert H, Borzym K, Langer I, Beck A, Lehrach H, Reinhardt R, Pohl TM, Eger P, Zimmermann W, Wedler H, Wambutt R, Purnelle B, Goffeau A, Cadieu E, Dr?ano S, Gloux S, Lelaure V, Mottier S, Galibert F, Aves SJ, Xiang Z, Hunt C, Moore K, Hurst SM, Lucas M, Rochet M, Gaillardin C, Tallada VA, Garzon A, Thode G, Daga RR, Cruzado L, Jimenez J, S?nchez M, del Rey F, Benito J, Dom?nguez A, Revuelta JL, Moreno S, Armstrong J, Forsburg SL, Cerutti L, Lowe T, McCombie WR, Paulsen I, Potashkin J, Shpakovski GV, Ussery D, Barrell BG, Nurse P, Cerrutti L
Nature415(6874)871-80(2002 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ppk29 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ppk29 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OSc101542	NM_001355774.1 Latest version!	Schizosaccharomyces pombe Ark1/Prk1 family protein kinase Ppk29 (ppk29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OSj02357	NM_001021936.2 Latest version!	Schizosaccharomyces pombe 972h- Ark1/Prk1 family protein kinase Ppk29 (ppk29), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ppk29 ( NM_001355774.1 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_001342923.1 Schizosaccharomyces pombe Ark1/Prk1 family protein kinase Ppk29 (ppk29), mRNA.

Price & Availability↑

CloneID	OSc101542
Clone ID Related Accession (Same CDS sequence)	NM_001355774.1
Accession Version	NM_001355774.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-04-02
Organism	Schizosaccharomyces pombe(fission yeast)
Product	Ark1/Prk1 family protein kinase Ppk29
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423).

ATGTCTGTTG CATCCTTTCT CACGGGCGAA AAGCTTCTAG CAGGATCCAA GTTTGTAATT 
GGAAAATACA ATGTTACTAT CGAAAAGTAC ATAGCCGAAG GCGGCTTTTC TCATGTATAT 
CTTGTTCAAA CAAATTCCAA AACTGATGGA TCACCGATAA CTGCTGTTTT GAAACGCATG 
TATTCTCCAG ACGAGAATGC CCTGCGTTTC GTGAAAACTG AAATCGAAAC GATGGAACTA 
CTCAAATCCA ATCCCCACGT GGTTTCGTAT ATCGACTCCT GCATATTTCC TCTCGAGAAA 
GCGGGTGTCA ACACTGGATT TGAAATCCTG CTTTTGATGG AATACTGCGC TGGGGGTGGA 
TTGATAGATT TTATGAATCA AAGGTTACAA ACTCGGTTGT CAGAGCATGA AGTCTTAAAA 
ATTATTTCAG ATATTGTACA GGGTGTTGCT TCCTTACACT ATCTGAGACC TCCTCTCATT 
CATCGCGATC TGAAAGTAGA AAATGTTTTG CTAAGCTTTA ATACTTTTAA ATTGTGCGAT 
TTTGGAAGTG TGACTGAGCC AATGCATGCT GCTGAAAACT CTAGCGAAAT TCAAGCATTA 
GAAAAAAGTA TCAACACATT TACTACCTAC CAATATCGTG CCCCTGAGAT GATTAATTTG 
TATTCAGGCT TAGGAATTGA TGAGAAAAGT GACATGTGGG CTTTAGGAGT CTTGTTATAC 
AAACTCTGTT ACTATACAAC TCCTTTTGAA ACACAAGGTC CTAACGCTAT ATTAACAGCT 
TCATATTCCT TCCCCCCATT TCCTCCTTAT TCACATTCTC TTAAAAATGT CATCATCGCT 
TTACTTCAGC CGAATCCTTG TCTTCGTCCA AATATTTTTC AGTTGATGTG TGAGATATGT 
CGTCTTAGAG GGACGGCTTT ACCTTTTAGG GATATATACA GTGGAAGAGA GTCGTCTTTT 
TATGATTTGC ATAACCGGAA AATAATGTCT TTGCTTCGAC AAACAACAAC AACACCGTTT 
GCTGGAAACC AAGTCATGCC GACTCCACAG GTTCAGGGCT CAAGACCTCC CCCTCCTCCT 
CCTATGCCAG CTCCTATCTA TAACGTACCA AATGTGCCGA CTGTACCAAC CGTTTCCCCT 
AATCCATTTT TGCAACCTAC TTTCTCTGGC ACTTCTCAAG CTAGTGCACA TCCTTCTATC 
CACAGCCAAT CCGCAACATT TAACAACACT CAAGATGTGA ACAGACAGAG TGTTTCGTCT 
CCGAAGGTTG TTTCATCAAG TTCTGTAACT GGCTCAGGAG CTAGTGGTCG CAAAGTCTAC 
ACACCTCAAA GTAAGAATCC CTTCCCAATA TCTCAGGGAG TCACGGGAAC ATCTTCAAAA 
TTTTCTGCTA GCGAGGTTAC AGAAGATAAT GGTTCACCAA ACGCCGGACT CGATGCCGAT 
AACGTTGACG TTGAGGAAGT TGTTAACCAG CGTTATCCTG ATCTAAATGA GTTGGAGTCA 
CAGCTTACTA GTCATACTAA TATTACGACT AGTTCTCATC ATAACCCTAG GGAACAAGAG 
ATAGCAAAAT TGGCTGATGA TGCATTCGCT AGTTTCCGTC AGACAGCTGG TACTTCAACC 
GCAAATAGTA CTCATACCAC TGGTACTCAG GATATATCTG CCTTTGATAC AAATTTTTCG 
GAAGGGGTTG GTAGCGTAAG GTCTTCTTTT GATTTCCCTG ATCGTCCAGT ATCTAGACGA 
CCTTCAAGCA CTTCTAGGTC GTCTGTTAAA ATTACTGGAC CTTCGAGCCC CAATGCGCGT 
TCTATGGCTT ATGAGCAACA CTCCTTTAAC CTTGATGATT TGAGTAATAC TAATTTTGGT 
TCTAATAATT CTCTTAGACA TGCCAAATCA TTTTCCAAAT ATTATTCACC TTCTCATAAA 
GATTCGAACA AAACTTCCAG AAACACATCT AAAGAGGGGC TCCCCTCCAG TCCTACTATG 
CTCTATCGTA CAACATCTAA TACTAGAGGT GACTATATCG TTCAACGCAC ACAACAGTCT 
GCCAATCCTT TGACAAATAT CGAACCCCAA GATATGTCAA ACCTTAGTAC TGATATTAAT 
GCTTCTGACG TTGTTGCCGA TTCAACCAAC TCTATTCTTT ATCCTACTTC GACCGCGTCT 
TCTGTTGCCA ACACTATTGC CTCCGATGTT GACGACTTTG GAACTTTTAA TGGTTTACAA 
AGAACCGTGA GTTTACATAG TGAGCGGGCA AGTCTTGACA AGAAGCGTCC TTCTTTTCAT 
GACAAAAATC CGTACTTTAA ATTTTCTCCA AGCAAGTATG TTGACGAAGG ATTGGAAGGT 
GAAAGTGAAT TAAGAGAGCG TGTACGAAGT TATGCTAACC GCTCAAGTTC AAGTTTCCAT 
AATCCAGATT TAACAGACAC AGAAATTCAA GAGACGTCTT TTAATAACGA TTTGAGGCCT 
GTAGCTTCTA TTCAAAGTGC AGAAACCTCC AGAAGCGTAA GTGATAGACC TGCAAATTTT 
CCAGAGGAAT TAAACGACAC GTTATTTGAG CAAAAATATC CGGGAATTGT TGATTACGAG 
GAACCCAATT CGAATGCAGA AAAGAACGTT AATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001342923.1
CDS	208..2826
Translation	MSVASFLTGEKLLAGSKFVIGKYNVTIEKYIAEGGFSHVYLVQTNSKTDGSPITAVLKRMYSPDENALRFVKTEIETMELLKSNPHVVSYIDSCIFPLEKAGVNTGFEILLLMEYCAGGGLIDFMNQRLQTRLSEHEVLKIISDIVQGVASLHYLRPPLIHRDLKVENVLLSFNTFKLCDFGSVTEPMHAAENSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDEKSDMWALGVLLYKLCYYTTPFETQGPNAILTASYSFPPFPPYSHSLKNVIIALLQPNPCLRPNIFQLMCEICRLRGTALPFRDIYSGRESSFYDLHNRKIMSLLRQTTTTPFAGNQVMPTPQVQGSRPPPPPPMPAPIYNVPNVPTVPTVSPNPFLQPTFSGTSQASAHPSIHSQSATFNNTQDVNRQSVSSPKVVSSSSVTGSGASGRKVYTPQSKNPFPISQGVTGTSSKFSASEVTEDNGSPNAGLDADNVDVEEVVNQRYPDLNELESQLTSHTNITTSSHHNPREQEIAKLADDAFASFRQTAGTSTANSTHTTGTQDISAFDTNFSEGVGSVRSSFDFPDRPVSRRPSSTSRSSVKITGPSSPNARSMAYEQHSFNLDDLSNTNFGSNNSLRHAKSFSKYYSPSHKDSNKTSRNTSKEGLPSSPTMLYRTTSNTRGDYIVQRTQQSANPLTNIEPQDMSNLSTDINASDVVADSTNSILYPTSTASSVANTIASDVDDFGTFNGLQRTVSLHSERASLDKKRPSFHDKNPYFKFSPSKYVDEGLEGESELRERVRSYANRSSSSFHNPDLTDTEIQETSFNNDLRPVASIQSAETSRSVSDRPANFPEELNDTLFEQKYPGIVDYEEPNSNAEKNVNI

Target ORF information:

RefSeq Version	NM_001355774.1
Organism	Schizosaccharomyces pombe(fission yeast)
Definition	Schizosaccharomyces pombe Ark1/Prk1 family protein kinase Ppk29 (ppk29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001355774.1

ORF Insert Sequence:

ATGTCTGTTG CATCCTTTCT CACGGGCGAA AAGCTTCTAG CAGGATCCAA GTTTGTAATT 
GGAAAATACA ATGTTACTAT CGAAAAGTAC ATAGCCGAAG GCGGCTTTTC TCATGTATAT 
CTTGTTCAAA CAAATTCCAA AACTGATGGA TCACCGATAA CTGCTGTTTT GAAACGCATG 
TATTCTCCAG ACGAGAATGC CCTGCGTTTC GTGAAAACTG AAATCGAAAC GATGGAACTA 
CTCAAATCCA ATCCCCACGT GGTTTCGTAT ATCGACTCCT GCATATTTCC TCTCGAGAAA 
GCGGGTGTCA ACACTGGATT TGAAATCCTG CTTTTGATGG AATACTGCGC TGGGGGTGGA 
TTGATAGATT TTATGAATCA AAGGTTACAA ACTCGGTTGT CAGAGCATGA AGTCTTAAAA 
ATTATTTCAG ATATTGTACA GGGTGTTGCT TCCTTACACT ATCTGAGACC TCCTCTCATT 
CATCGCGATC TGAAAGTAGA AAATGTTTTG CTAAGCTTTA ATACTTTTAA ATTGTGCGAT 
TTTGGAAGTG TGACTGAGCC AATGCATGCT GCTGAAAACT CTAGCGAAAT TCAAGCATTA 
GAAAAAAGTA TCAACACATT TACTACCTAC CAATATCGTG CCCCTGAGAT GATTAATTTG 
TATTCAGGCT TAGGAATTGA TGAGAAAAGT GACATGTGGG CTTTAGGAGT CTTGTTATAC 
AAACTCTGTT ACTATACAAC TCCTTTTGAA ACACAAGGTC CTAACGCTAT ATTAACAGCT 
TCATATTCCT TCCCCCCATT TCCTCCTTAT TCACATTCTC TTAAAAATGT CATCATCGCT 
TTACTTCAGC CGAATCCTTG TCTTCGTCCA AATATTTTTC AGTTGATGTG TGAGATATGT 
CGTCTTAGAG GGACGGCTTT ACCTTTTAGG GATATATACA GTGGAAGAGA GTCGTCTTTT 
TATGATTTGC ATAACCGGAA AATAATGTCT TTGCTTCGAC AAACAACAAC AACACCGTTT 
GCTGGAAACC AAGTCATGCC GACTCCACAG GTTCAGGGCT CAAGACCTCC CCCTCCTCCT 
CCTATGCCAG CTCCTATCTA TAACGTACCA AATGTGCCGA CTGTACCAAC CGTTTCCCCT 
AATCCATTTT TGCAACCTAC TTTCTCTGGC ACTTCTCAAG CTAGTGCACA TCCTTCTATC 
CACAGCCAAT CCGCAACATT TAACAACACT CAAGATGTGA ACAGACAGAG TGTTTCGTCT 
CCGAAGGTTG TTTCATCAAG TTCTGTAACT GGCTCAGGAG CTAGTGGTCG CAAAGTCTAC 
ACACCTCAAA GTAAGAATCC CTTCCCAATA TCTCAGGGAG TCACGGGAAC ATCTTCAAAA 
TTTTCTGCTA GCGAGGTTAC AGAAGATAAT GGTTCACCAA ACGCCGGACT CGATGCCGAT 
AACGTTGACG TTGAGGAAGT TGTTAACCAG CGTTATCCTG ATCTAAATGA GTTGGAGTCA 
CAGCTTACTA GTCATACTAA TATTACGACT AGTTCTCATC ATAACCCTAG GGAACAAGAG 
ATAGCAAAAT TGGCTGATGA TGCATTCGCT AGTTTCCGTC AGACAGCTGG TACTTCAACC 
GCAAATAGTA CTCATACCAC TGGTACTCAG GATATATCTG CCTTTGATAC AAATTTTTCG 
GAAGGGGTTG GTAGCGTAAG GTCTTCTTTT GATTTCCCTG ATCGTCCAGT ATCTAGACGA 
CCTTCAAGCA CTTCTAGGTC GTCTGTTAAA ATTACTGGAC CTTCGAGCCC CAATGCGCGT 
TCTATGGCTT ATGAGCAACA CTCCTTTAAC CTTGATGATT TGAGTAATAC TAATTTTGGT 
TCTAATAATT CTCTTAGACA TGCCAAATCA TTTTCCAAAT ATTATTCACC TTCTCATAAA 
GATTCGAACA AAACTTCCAG AAACACATCT AAAGAGGGGC TCCCCTCCAG TCCTACTATG 
CTCTATCGTA CAACATCTAA TACTAGAGGT GACTATATCG TTCAACGCAC ACAACAGTCT 
GCCAATCCTT TGACAAATAT CGAACCCCAA GATATGTCAA ACCTTAGTAC TGATATTAAT 
GCTTCTGACG TTGTTGCCGA TTCAACCAAC TCTATTCTTT ATCCTACTTC GACCGCGTCT 
TCTGTTGCCA ACACTATTGC CTCCGATGTT GACGACTTTG GAACTTTTAA TGGTTTACAA 
AGAACCGTGA GTTTACATAG TGAGCGGGCA AGTCTTGACA AGAAGCGTCC TTCTTTTCAT 
GACAAAAATC CGTACTTTAA ATTTTCTCCA AGCAAGTATG TTGACGAAGG ATTGGAAGGT 
GAAAGTGAAT TAAGAGAGCG TGTACGAAGT TATGCTAACC GCTCAAGTTC AAGTTTCCAT 
AATCCAGATT TAACAGACAC AGAAATTCAA GAGACGTCTT TTAATAACGA TTTGAGGCCT 
GTAGCTTCTA TTCAAAGTGC AGAAACCTCC AGAAGCGTAA GTGATAGACC TGCAAATTTT 
CCAGAGGAAT TAAACGACAC GTTATTTGAG CAAAAATATC CGGGAATTGT TGATTACGAG 
GAACCCAATT CGAATGCAGA AAAGAACGTT AATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ppk29 ( NM_001021936.2 ) cDNA ORF clone, Schizosaccharomyces pombe(fission yeast) -> NP_596027.1 Schizosaccharomyces pombe 972h- Ark1/Prk1 family protein kinase Ppk29 (ppk29), mRNA.

Price & Availability↑

CloneID	OSj02357
Clone ID Related Accession (Same CDS sequence)	NM_001021936.2
Accession Version	NM_001021936.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2619bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2013-04-30
Organism	Schizosaccharomyces pombe 972h-
Product	Ark1/Prk1 family protein kinase Ppk29
Comment	PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. This record is derived from an annotated genomic sequence (NC_003423). On Dec 10, 2012 this sequence version replaced NM_001021936.1. Notes: S. pombe (fission yeast) sequence and annotation are available from: (URL, http://www.genedb.org/genedb/pombe/index.jsp) (URL, http://www.sanger.ac.uk/Projects/S_pombe/) CDS are numbered using the following system eg SPAC5H10.01c. SP (S. pombe), A (chromosome 1), c5H10 (cosmid name), .01 (first CDS), c (complementary strand). However, clones may have been reorientated since the original submission, therefore the complementary strand notation may be invalid for strand inference.

ATGTCTGTTG CATCCTTTCT CACGGGCGAA AAGCTTCTAG CAGGATCCAA GTTTGTAATT 
GGAAAATACA ATGTTACTAT CGAAAAGTAC ATAGCCGAAG GCGGCTTTTC TCATGTATAT 
CTTGTTCAAA CAAATTCCAA AACTGATGGA TCACCGATAA CTGCTGTTTT GAAACGCATG 
TATTCTCCAG ACGAGAATGC CCTGCGTTTC GTGAAAACTG AAATCGAAAC GATGGAACTA 
CTCAAATCCA ATCCCCACGT GGTTTCGTAT ATCGACTCCT GCATATTTCC TCTCGAGAAA 
GCGGGTGTCA ACACTGGATT TGAAATCCTG CTTTTGATGG AATACTGCGC TGGGGGTGGA 
TTGATAGATT TTATGAATCA AAGGTTACAA ACTCGGTTGT CAGAGCATGA AGTCTTAAAA 
ATTATTTCAG ATATTGTACA GGGTGTTGCT TCCTTACACT ATCTGAGACC TCCTCTCATT 
CATCGCGATC TGAAAGTAGA AAATGTTTTG CTAAGCTTTA ATACTTTTAA ATTGTGCGAT 
TTTGGAAGTG TGACTGAGCC AATGCATGCT GCTGAAAACT CTAGCGAAAT TCAAGCATTA 
GAAAAAAGTA TCAACACATT TACTACCTAC CAATATCGTG CCCCTGAGAT GATTAATTTG 
TATTCAGGCT TAGGAATTGA TGAGAAAAGT GACATGTGGG CTTTAGGAGT CTTGTTATAC 
AAACTCTGTT ACTATACAAC TCCTTTTGAA ACACAAGGTC CTAACGCTAT ATTAACAGCT 
TCATATTCCT TCCCCCCATT TCCTCCTTAT TCACATTCTC TTAAAAATGT CATCATCGCT 
TTACTTCAGC CGAATCCTTG TCTTCGTCCA AATATTTTTC AGTTGATGTG TGAGATATGT 
CGTCTTAGAG GGACGGCTTT ACCTTTTAGG GATATATACA GTGGAAGAGA GTCGTCTTTT 
TATGATTTGC ATAACCGGAA AATAATGTCT TTGCTTCGAC AAACAACAAC AACACCGTTT 
GCTGGAAACC AAGTCATGCC GACTCCACAG GTTCAGGGCT CAAGACCTCC CCCTCCTCCT 
CCTATGCCAG CTCCTATCTA TAACGTACCA AATGTGCCGA CTGTACCAAC CGTTTCCCCT 
AATCCATTTT TGCAACCTAC TTTCTCTGGC ACTTCTCAAG CTAGTGCACA TCCTTCTATC 
CACAGCCAAT CCGCAACATT TAACAACACT CAAGATGTGA ACAGACAGAG TGTTTCGTCT 
CCGAAGGTTG TTTCATCAAG TTCTGTAACT GGCTCAGGAG CTAGTGGTCG CAAAGTCTAC 
ACACCTCAAA GTAAGAATCC CTTCCCAATA TCTCAGGGAG TCACGGGAAC ATCTTCAAAA 
TTTTCTGCTA GCGAGGTTAC AGAAGATAAT GGTTCACCAA ACGCCGGACT CGATGCCGAT 
AACGTTGACG TTGAGGAAGT TGTTAACCAG CGTTATCCTG ATCTAAATGA GTTGGAGTCA 
CAGCTTACTA GTCATACTAA TATTACGACT AGTTCTCATC ATAACCCTAG GGAACAAGAG 
ATAGCAAAAT TGGCTGATGA TGCATTCGCT AGTTTCCGTC AGACAGCTGG TACTTCAACC 
GCAAATAGTA CTCATACCAC TGGTACTCAG GATATATCTG CCTTTGATAC AAATTTTTCG 
GAAGGGGTTG GTAGCGTAAG GTCTTCTTTT GATTTCCCTG ATCGTCCAGT ATCTAGACGA 
CCTTCAAGCA CTTCTAGGTC GTCTGTTAAA ATTACTGGAC CTTCGAGCCC CAATGCGCGT 
TCTATGGCTT ATGAGCAACA CTCCTTTAAC CTTGATGATT TGAGTAATAC TAATTTTGGT 
TCTAATAATT CTCTTAGACA TGCCAAATCA TTTTCCAAAT ATTATTCACC TTCTCATAAA 
GATTCGAACA AAACTTCCAG AAACACATCT AAAGAGGGGC TCCCCTCCAG TCCTACTATG 
CTCTATCGTA CAACATCTAA TACTAGAGGT GACTATATCG TTCAACGCAC ACAACAGTCT 
GCCAATCCTT TGACAAATAT CGAACCCCAA GATATGTCAA ACCTTAGTAC TGATATTAAT 
GCTTCTGACG TTGTTGCCGA TTCAACCAAC TCTATTCTTT ATCCTACTTC GACCGCGTCT 
TCTGTTGCCA ACACTATTGC CTCCGATGTT GACGACTTTG GAACTTTTAA TGGTTTACAA 
AGAACCGTGA GTTTACATAG TGAGCGGGCA AGTCTTGACA AGAAGCGTCC TTCTTTTCAT 
GACAAAAATC CGTACTTTAA ATTTTCTCCA AGCAAGTATG TTGACGAAGG ATTGGAAGGT 
GAAAGTGAAT TAAGAGAGCG TGTACGAAGT TATGCTAACC GCTCAAGTTC AAGTTTCCAT 
AATCCAGATT TAACAGACAC AGAAATTCAA GAGACGTCTT TTAATAACGA TTTGAGGCCT 
GTAGCTTCTA TTCAAAGTGC AGAAACCTCC AGAAGCGTAA GTGATAGACC TGCAAATTTT 
CCAGAGGAAT TAAACGACAC GTTATTTGAG CAAAAATATC CGGGAATTGT TGATTACGAG 
GAACCCAATT CGAATGCAGA AAAGAACGTT AATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_596027.1
CDS	246..2864
Translation	MSVASFLTGEKLLAGSKFVIGKYNVTIEKYIAEGGFSHVYLVQTNSKTDGSPITAVLKRMYSPDENALRFVKTEIETMELLKSNPHVVSYIDSCIFPLEKAGVNTGFEILLLMEYCAGGGLIDFMNQRLQTRLSEHEVLKIISDIVQGVASLHYLRPPLIHRDLKVENVLLSFNTFKLCDFGSVTEPMHAAENSSEIQALEKSINTFTTYQYRAPEMINLYSGLGIDEKSDMWALGVLLYKLCYYTTPFETQGPNAILTASYSFPPFPPYSHSLKNVIIALLQPNPCLRPNIFQLMCEICRLRGTALPFRDIYSGRESSFYDLHNRKIMSLLRQTTTTPFAGNQVMPTPQVQGSRPPPPPPMPAPIYNVPNVPTVPTVSPNPFLQPTFSGTSQASAHPSIHSQSATFNNTQDVNRQSVSSPKVVSSSSVTGSGASGRKVYTPQSKNPFPISQGVTGTSSKFSASEVTEDNGSPNAGLDADNVDVEEVVNQRYPDLNELESQLTSHTNITTSSHHNPREQEIAKLADDAFASFRQTAGTSTANSTHTTGTQDISAFDTNFSEGVGSVRSSFDFPDRPVSRRPSSTSRSSVKITGPSSPNARSMAYEQHSFNLDDLSNTNFGSNNSLRHAKSFSKYYSPSHKDSNKTSRNTSKEGLPSSPTMLYRTTSNTRGDYIVQRTQQSANPLTNIEPQDMSNLSTDINASDVVADSTNSILYPTSTASSVANTIASDVDDFGTFNGLQRTVSLHSERASLDKKRPSFHDKNPYFKFSPSKYVDEGLEGESELRERVRSYANRSSSSFHNPDLTDTEIQETSFNNDLRPVASIQSAETSRSVSDRPANFPEELNDTLFEQKYPGIVDYEEPNSNAEKNVNI

Target ORF information:

RefSeq Version	NM_001021936.2
Organism	Schizosaccharomyces pombe 972h-
Definition	Schizosaccharomyces pombe 972h- Ark1/Prk1 family protein kinase Ppk29 (ppk29), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001021936.2

ORF Insert Sequence:

ATGTCTGTTG CATCCTTTCT CACGGGCGAA AAGCTTCTAG CAGGATCCAA GTTTGTAATT 
GGAAAATACA ATGTTACTAT CGAAAAGTAC ATAGCCGAAG GCGGCTTTTC TCATGTATAT 
CTTGTTCAAA CAAATTCCAA AACTGATGGA TCACCGATAA CTGCTGTTTT GAAACGCATG 
TATTCTCCAG ACGAGAATGC CCTGCGTTTC GTGAAAACTG AAATCGAAAC GATGGAACTA 
CTCAAATCCA ATCCCCACGT GGTTTCGTAT ATCGACTCCT GCATATTTCC TCTCGAGAAA 
GCGGGTGTCA ACACTGGATT TGAAATCCTG CTTTTGATGG AATACTGCGC TGGGGGTGGA 
TTGATAGATT TTATGAATCA AAGGTTACAA ACTCGGTTGT CAGAGCATGA AGTCTTAAAA 
ATTATTTCAG ATATTGTACA GGGTGTTGCT TCCTTACACT ATCTGAGACC TCCTCTCATT 
CATCGCGATC TGAAAGTAGA AAATGTTTTG CTAAGCTTTA ATACTTTTAA ATTGTGCGAT 
TTTGGAAGTG TGACTGAGCC AATGCATGCT GCTGAAAACT CTAGCGAAAT TCAAGCATTA 
GAAAAAAGTA TCAACACATT TACTACCTAC CAATATCGTG CCCCTGAGAT GATTAATTTG 
TATTCAGGCT TAGGAATTGA TGAGAAAAGT GACATGTGGG CTTTAGGAGT CTTGTTATAC 
AAACTCTGTT ACTATACAAC TCCTTTTGAA ACACAAGGTC CTAACGCTAT ATTAACAGCT 
TCATATTCCT TCCCCCCATT TCCTCCTTAT TCACATTCTC TTAAAAATGT CATCATCGCT 
TTACTTCAGC CGAATCCTTG TCTTCGTCCA AATATTTTTC AGTTGATGTG TGAGATATGT 
CGTCTTAGAG GGACGGCTTT ACCTTTTAGG GATATATACA GTGGAAGAGA GTCGTCTTTT 
TATGATTTGC ATAACCGGAA AATAATGTCT TTGCTTCGAC AAACAACAAC AACACCGTTT 
GCTGGAAACC AAGTCATGCC GACTCCACAG GTTCAGGGCT CAAGACCTCC CCCTCCTCCT 
CCTATGCCAG CTCCTATCTA TAACGTACCA AATGTGCCGA CTGTACCAAC CGTTTCCCCT 
AATCCATTTT TGCAACCTAC TTTCTCTGGC ACTTCTCAAG CTAGTGCACA TCCTTCTATC 
CACAGCCAAT CCGCAACATT TAACAACACT CAAGATGTGA ACAGACAGAG TGTTTCGTCT 
CCGAAGGTTG TTTCATCAAG TTCTGTAACT GGCTCAGGAG CTAGTGGTCG CAAAGTCTAC 
ACACCTCAAA GTAAGAATCC CTTCCCAATA TCTCAGGGAG TCACGGGAAC ATCTTCAAAA 
TTTTCTGCTA GCGAGGTTAC AGAAGATAAT GGTTCACCAA ACGCCGGACT CGATGCCGAT 
AACGTTGACG TTGAGGAAGT TGTTAACCAG CGTTATCCTG ATCTAAATGA GTTGGAGTCA 
CAGCTTACTA GTCATACTAA TATTACGACT AGTTCTCATC ATAACCCTAG GGAACAAGAG 
ATAGCAAAAT TGGCTGATGA TGCATTCGCT AGTTTCCGTC AGACAGCTGG TACTTCAACC 
GCAAATAGTA CTCATACCAC TGGTACTCAG GATATATCTG CCTTTGATAC AAATTTTTCG 
GAAGGGGTTG GTAGCGTAAG GTCTTCTTTT GATTTCCCTG ATCGTCCAGT ATCTAGACGA 
CCTTCAAGCA CTTCTAGGTC GTCTGTTAAA ATTACTGGAC CTTCGAGCCC CAATGCGCGT 
TCTATGGCTT ATGAGCAACA CTCCTTTAAC CTTGATGATT TGAGTAATAC TAATTTTGGT 
TCTAATAATT CTCTTAGACA TGCCAAATCA TTTTCCAAAT ATTATTCACC TTCTCATAAA 
GATTCGAACA AAACTTCCAG AAACACATCT AAAGAGGGGC TCCCCTCCAG TCCTACTATG 
CTCTATCGTA CAACATCTAA TACTAGAGGT GACTATATCG TTCAACGCAC ACAACAGTCT 
GCCAATCCTT TGACAAATAT CGAACCCCAA GATATGTCAA ACCTTAGTAC TGATATTAAT 
GCTTCTGACG TTGTTGCCGA TTCAACCAAC TCTATTCTTT ATCCTACTTC GACCGCGTCT 
TCTGTTGCCA ACACTATTGC CTCCGATGTT GACGACTTTG GAACTTTTAA TGGTTTACAA 
AGAACCGTGA GTTTACATAG TGAGCGGGCA AGTCTTGACA AGAAGCGTCC TTCTTTTCAT 
GACAAAAATC CGTACTTTAA ATTTTCTCCA AGCAAGTATG TTGACGAAGG ATTGGAAGGT 
GAAAGTGAAT TAAGAGAGCG TGTACGAAGT TATGCTAACC GCTCAAGTTC AAGTTTCCAT 
AATCCAGATT TAACAGACAC AGAAATTCAA GAGACGTCTT TTAATAACGA TTTGAGGCCT 
GTAGCTTCTA TTCAAAGTGC AGAAACCTCC AGAAGCGTAA GTGATAGACC TGCAAATTTT 
CCAGAGGAAT TAAACGACAC GTTATTTGAG CAAAAATATC CGGGAATTGT TGATTACGAG 
GAACCCAATT CGAATGCAGA AAAGAACGTT AATATTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The genome sequence of Schizosaccharomyces pombe.
Nature415(6874)871-80(2002 Feb)
Wood V,Gwilliam R,Rajandream MA,Lyne M,Lyne R,Stewart A,Sgouros J,Peat N,Hayles J,Baker S,Basham D,Bowman S,Brooks K,Brown D,Brown S,Chillingworth T,Churcher C,Collins M,Connor R,Cronin A,Davis P,Feltwell T,Fraser A,Gentles S,Goble A,Hamlin N,Harris D,Hidalgo J,Hodgson G,Holroyd S,Hornsby T,Howarth S,Huckle EJ,Hunt S,Jagels K,James K,Jones L,Jones M,Leather S,McDonald S,McLean J,Mooney P,Moule S,Mungall K,Murphy L,Niblett D,Odell C,Oliver K,O'Neil S,Pearson D,Quail MA,Rabbinowitsch E,Rutherford K,Rutter S,Saunders D,Seeger K,Sharp S,Skelton J,Simmonds M,Squares R,Squares S,Stevens K,Taylor K,Taylor RG,Tivey A,Walsh S,Warren T,Whitehead S,Woodward J,Volckaert G,Aert R,Robben J,Grymonprez B,Weltjens I,Vanstreels E,Rieger M,Sch?fer M,M?ller-Auer S,Gabel C,Fuchs M,D?sterh?ft A,Fritzc C,Holzer E,Moestl D,Hilbert H,Borzym K,Langer I,Beck A,Lehrach H,Reinhardt R,Pohl TM,Eger P,Zimmermann W,Wedler H,Wambutt R,Purnelle B,Goffeau A,Cadieu E,Dr?ano S,Gloux S,Lelaure V,Mottier S,Galibert F,Aves SJ,Xiang Z,Hunt C,Moore K,Hurst SM,Lucas M,Rochet M,Gaillardin C,Tallada VA,Garzon A,Thode G,Daga RR,Cruzado L,Jimenez J,S?nchez M,del Rey F,Benito J,Dom?nguez A,Revuelta JL,Moreno S,Armstrong J,Forsburg SL,Cerutti L,Lowe T,McCombie WR,Paulsen I,Potashkin J,Shpakovski GV,Ussery D,Barrell BG,Nurse P,Cerrutti L

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ppk29 cDNA ORF clone, Schizosaccharomyces pombe(fission yeast)

Gene

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gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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